Bhi03G000622 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000622
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionL-ascorbate peroxidase
Locationchr3: 15406187 .. 15410130 (+)
RNA-Seq ExpressionBhi03G000622
SyntenyBhi03G000622
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAAAATATACATTTCTCTGCCTCCGCCGCGAAGCAAAGAACCCAATGCTTTCCTTGGCCCTCTCGTACACAGAGAATCGCACCAAAAACTCCCTTTCTCCTTCTTCATTTCTTCTCAATTTAAACCCAAAATCTGAACTCCGACGAATAATGCCATCAATTTTTGTTCAATTTGTTGGCAAAGAAAGTGAAATTTCTGCATTCTTATCAAGATATCAATGGCGCCTCCCAAAGTCGATGCTGCGTACCTCAAGGAAATCGAGAAGGCCCGTCGAGACCTTCGTGCTCTAATCTTCAAGGAGAAATGCGCTCCTATTATGCTTCGTTTAGCGTAAGTTCTTGTTGTGAGTTCTATATTTGTAGTCATTCCGAAATTTGCAGGGGAGTGTAATAATAGAAATTATTTGGCATTTTGTTTTTCTTTTACTGCACAGTTGATTGGGTTTTGTAGTTGATGACTTATCCTCTTTTGTCGAGTTCAGAATCACTATGAAATGTCCATTTAATCATCCAACAATATTTTTGAGTTCAAAATGCTTTTGATTCTTGAGCTTTTTATGTGAGTAACTATTTAGTTTATTCACCTCAACTTTAGTGGTAAAGATTTAGATTTTTGTAATCTTAAGTTTGTAACACTTTTTAACTTTAATAATTAACCATCTAAATCTTGTGCTTTTCAATTTGACATGATTTAGTACTTGACATGCAAAATATTATTAGATTTGATAAAATATTTTACACATGTAGATTGATAAATTGATAAAGGATCAGTTTTGTTTATAAACTATAAAAACTAAATCGTTAGATAATAAAAAAATGACACTTAATTTTGATAAGGTATACATATGATCTAAATTGTTACAAATTTGAAATTATAGTGACTAAATTGTTAAATGCAAAAGTTTAAGAAGTAAATATTATTACAAAATTTGAAAGTATAGGGATGAAATACTTGCAAATTAAAGTTAAGAGACTAAACTATCACTTGTTAAAAGTTTGGGAACAAAAGTGTTTTAACCTAAATTTAATATTTGATTTAATGCGAATTTTCCACTATCATTAAAATTAGTTTTGGATGAATAAAAGTATGCCTTCAGTGATATCATTAAAATCACTCTCAAACATATACTTATATTCATTGCTAGAACTCATTTGTTGAAGAGGGATTTTTGTTGGGTAGGTGGCATGATGCTGGTACTTACGATGCTAAAACGAAAACCGGAGGTCCAGATGGCTCCATCAGGTTCATGGATGAATTGAACCATACTGCAAACAAGGGACTGAAAATCGCAGTTGATTTCTGTGGTGAGCTTTTTTCACCTCTCTTTATTGTAAGTCTATATATAGAGTTGAGTTATTGTCCTTCAAATATTAGTTTCAATGTAATGACAAAATTAATTAATCTCTGTCCCAACTGATTTGATTGTCTGGGGATTTGATTTTATTTGCTTTGGCAGACATTTTTACTCATTCTTCCTTGTGTTGTCACTAAACAGAAACTGTGAAGGCCAGACATCCAAAAATCACATATGCAGATCTGTACCAGGTGGTTTCCTTTCATTGAAGTCGTCCTTTTTGGACTATTTCTATCCTCCAGTTCTCCGTGACATTCATCGACATAATTTTCTTTTTCTAATTGATGCTATTCTGCTGTGTGTTGGAACTTATATGTTTTGGAAATGGGTGGCAGTTTCTTCTTTTCAAATCACCATCGAACCCAAAAGCTTAAAGCTAACGGTTACTTTGACACTCCTCATTTGTTGGCTTGACTTGAAAATATTTAGAAGACCCAACAAATAGAAATCAACATTAATTAGGAAACAAGTGATATTACATGGGTTTGAATACAAAACTTCCCACTCTAATACCATGTTGAATCACCACTGAACTCAAAAGCTTAAGTTGATGGGTTATAGTAAATTTAATTATATCAAATACTTCAACACTTGTTCACCTGTTGTTGCTGCTCGTCGCTCCAAACAATTAGGAAATAAGAATTTTTCGAAATTAAAAGAACATAATGATGTAAAGTTCCAGGCTTTCAGCTGCCAAGATTTGGCTCATTCTCCTCTAATTGTAAGTTTTCAGCTTGCTGGAGTTGTTGCTGTGGAGATCACTGGCGGGCCAACCATTGACTTTGTCCCCGGAAGAAAGGTATCTGATGCACAAAAAATTTGATTGACGTTGACAATTGAGATTGCCGTTGAATAACTTCATTCTTCTTTTATATCAATGGATTAGGACTCGGATGTAGTTCCAGAAGAAGGCCGCCTTCCAGATGGCCACCAAGGTGACTTTTTGCTAAGGCATATATAGATATATTTAAGATATAAATAACTTCGTTGGTAATCTAATGATGGTTCAAATTAATTATTCAACTTTTAAAATATCTAGTTGAGTTTTTAAATATAAAATATAACAATGAATGTTTATCTTAGTTCCTCAACTTTGTAAAACAACTATTTTGGGCCTAGCAAAATTTTCCATCTAATATTCACTCATCCAACCTCTGTGGCTTTTAATAATCGCTCACTTCAGCAGATTCAGCATATCAAAGTTACCTTATCTATGAACAAATGTTTTTTTTTTCTCCCTATAATTAAATTAGTCATTTTGAAAAAATTAATGACAGAAATGAGATATAAACCTTTAATAATGGTAAGTGTTTCCTCAAAATAATAATAATCATAATAATAATAATAATGGTAAGTGTTCAAACTTCACGTGTGTTGGATCTTGTGCTAATTTTTAAGTCCACAAATGAAAAGGCCACAGGTTTAAGCTTTAAGCTTTTGGAGTGTCTAGAACTTTTTGTTCAAAGTTATAGCAGAAACCATGATTGAGTGATCATTACATGAAACTTCACTGCAGGTGCTTCCCATTTAAGAGATGTCTTTTACAGGATGGGACTAACTGACAAGGATATTGTGGCATTATCTGGAGGACACACATTGGTACTTTCTCTTGTTCCTTTCAACTATGAAGAAATTGGATCTTCACCCTTGGCTTTATATTTCCCTTTTCATTAATCATAGTTCTGGTTGCTAATTCTTCAGGGAAGAGCTCACAAGGATAGATCAGATTTTGAAGGCCCATGGACAAAGGACCCTCTGAAGTTTGATAACTCCTATTTCGTGTGAGAATAAGAAAATATATATATATATATATATATCCTCACTTCATGCATTCAATTCCCCCTTGTTTGTAGATAAAACAAGTTCTCATGCAAATGTAATTTTGATCAGGGAATTGCTGAAAAATGACTCTCGATCTAAGGAGAAACTATTGAAGCTTCCTACAGACAAGGCTTTGGTCGAGGATCCTCGGTTTTTCCAATATGTGAAGGCATATGCAAATGTGAGCTTCAACTGCTGTGCTTTTATCATGTTTGAGAGTGATTTTTAGAGTGATTTTATCAGGTTAAAAAAAGAAACTCACATTTAAAAGTGTAAAACCAAACATTAAATTGATTTTAAATCATTACATACACATTTTCAACCAAAGTGATTTTAATAATTTTAATTTCACTATAGAACTCATCTTTTAACTCGAAGGATTTTATGTATTTCTTTCCAATAGGATGAAGTCAAGTTTTTCGAGGATTATGCAGCATCTCACAAGAAACTGTCTGAGCTTGGCTTCACTACACCTTCCACGGTTCTCAAGAGCAGAGCCTCCTCATGGGCAAATAAAATTACTGTAGCTGCAATCGTAGCAGTTGTGGCGATACTGAGTTACAAAAGAGCAATGTCGCCTGAGGGCTTTCTTCCAAACCTCTTCTGATTATTCTAAAGAAAGATATTCTTACAACTATATATATATATGCATATATGTATGTTCATGGATAATTCAATGTTCCCATTTGTACATTTGTCTCTCGTTTTAAAATGGTCTGGGAATGAAAAAGAGAGCTTTAAAAGCTTTTTCCAGTTCTTATATAAG

mRNA sequence

ATTTAAAATATACATTTCTCTGCCTCCGCCGCGAAGCAAAGAACCCAATGCTTTCCTTGGCCCTCTCGTACACAGAGAATCGCACCAAAAACTCCCTTTCTCCTTCTTCATTTCTTCTCAATTTAAACCCAAAATCTGAACTCCGACGAATAATGCCATCAATTTTTGTTCAATTTGTTGGCAAAGAAAGTGAAATTTCTGCATTCTTATCAAGATATCAATGGCGCCTCCCAAAGTCGATGCTGCGTACCTCAAGGAAATCGAGAAGGCCCGTCGAGACCTTCGTGCTCTAATCTTCAAGGAGAAATGCGCTCCTATTATGCTTCGTTTAGCGTGGCATGATGCTGGTACTTACGATGCTAAAACGAAAACCGGAGGTCCAGATGGCTCCATCAGGTTCATGGATGAATTGAACCATACTGCAAACAAGGGACTGAAAATCGCAGTTGATTTCTGTGAAACTGTGAAGGCCAGACATCCAAAAATCACATATGCAGATCTGTACCAGCTTGCTGGAGTTGTTGCTGTGGAGATCACTGGCGGGCCAACCATTGACTTTGTCCCCGGAAGAAAGGACTCGGATGTAGTTCCAGAAGAAGGCCGCCTTCCAGATGGCCACCAAGGTGCTTCCCATTTAAGAGATGTCTTTTACAGGATGGGACTAACTGACAAGGATATTGTGGCATTATCTGGAGGACACACATTGGGAAGAGCTCACAAGGATAGATCAGATTTTGAAGGCCCATGGACAAAGGACCCTCTGAAGTTTGATAACTCCTATTTCGTGGAATTGCTGAAAAATGACTCTCGATCTAAGGAGAAACTATTGAAGCTTCCTACAGACAAGGCTTTGGTCGAGGATCCTCGGTTTTTCCAATATGTGAAGGCATATGCAAATGATGAAGTCAAGTTTTTCGAGGATTATGCAGCATCTCACAAGAAACTGTCTGAGCTTGGCTTCACTACACCTTCCACGGTTCTCAAGAGCAGAGCCTCCTCATGGGCAAATAAAATTACTGTAGCTGCAATCGTAGCAGTTGTGGCGATACTGAGTTACAAAAGAGCAATGTCGCCTGAGGGCTTTCTTCCAAACCTCTTCTGATTATTCTAAAGAAAGATATTCTTACAACTATATATATATATGCATATATGTATGTTCATGGATAATTCAATGTTCCCATTTGTACATTTGTCTCTCGTTTTAAAATGGTCTGGGAATGAAAAAGAGAGCTTTAAAAGCTTTTTCCAGTTCTTATATAAG

Coding sequence (CDS)

ATGGCGCCTCCCAAAGTCGATGCTGCGTACCTCAAGGAAATCGAGAAGGCCCGTCGAGACCTTCGTGCTCTAATCTTCAAGGAGAAATGCGCTCCTATTATGCTTCGTTTAGCGTGGCATGATGCTGGTACTTACGATGCTAAAACGAAAACCGGAGGTCCAGATGGCTCCATCAGGTTCATGGATGAATTGAACCATACTGCAAACAAGGGACTGAAAATCGCAGTTGATTTCTGTGAAACTGTGAAGGCCAGACATCCAAAAATCACATATGCAGATCTGTACCAGCTTGCTGGAGTTGTTGCTGTGGAGATCACTGGCGGGCCAACCATTGACTTTGTCCCCGGAAGAAAGGACTCGGATGTAGTTCCAGAAGAAGGCCGCCTTCCAGATGGCCACCAAGGTGCTTCCCATTTAAGAGATGTCTTTTACAGGATGGGACTAACTGACAAGGATATTGTGGCATTATCTGGAGGACACACATTGGGAAGAGCTCACAAGGATAGATCAGATTTTGAAGGCCCATGGACAAAGGACCCTCTGAAGTTTGATAACTCCTATTTCGTGGAATTGCTGAAAAATGACTCTCGATCTAAGGAGAAACTATTGAAGCTTCCTACAGACAAGGCTTTGGTCGAGGATCCTCGGTTTTTCCAATATGTGAAGGCATATGCAAATGATGAAGTCAAGTTTTTCGAGGATTATGCAGCATCTCACAAGAAACTGTCTGAGCTTGGCTTCACTACACCTTCCACGGTTCTCAAGAGCAGAGCCTCCTCATGGGCAAATAAAATTACTGTAGCTGCAATCGTAGCAGTTGTGGCGATACTGAGTTACAAAAGAGCAATGTCGCCTGAGGGCTTTCTTCCAAACCTCTTCTGA

Protein sequence

MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGFLPNLF
Homology
BLAST of Bhi03G000622 vs. TAIR 10
Match: AT4G35000.1 (ascorbate peroxidase 3 )

HSP 1 Score: 382.5 bits (981), Expect = 3.1e-106
Identity = 197/278 (70.86%), Postives = 221/278 (79.50%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MA P VDA YLKEI KARR+LR+LI  + CAPIMLRLAWHDAGTYDA++KTGGP+GSIR 
Sbjct: 1   MAAPIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRN 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
            +E  H AN GLKIA+D CE VKA+HPKITYADLYQLAGVVAVE+TGGP I FVPGRKDS
Sbjct: 61  EEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +V P+EGRLPD  QG  HLRDVFYRMGL+DKDIVALSGGHTLGRAH +RS F+GPWT++P
Sbjct: 121 NVCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLK +S   E LLKLPTDK L+EDP F + V+ YA DE  FF DYA SHK
Sbjct: 181 LKFDNSYFVELLKGES---EGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHK 240

Query: 241 KLSELGFTTPSTVLKSRASSW---ANKITVAAIVAVVA 276
           KLSELGF   S+  K+ A S     +   VA   AVVA
Sbjct: 241 KLSELGFNPNSSAGKAVADSTILAQSAFGVAVAAAVVA 275

BLAST of Bhi03G000622 vs. TAIR 10
Match: AT4G35970.1 (ascorbate peroxidase 5 )

HSP 1 Score: 360.9 bits (925), Expect = 9.6e-100
Identity = 183/271 (67.53%), Postives = 212/271 (78.23%), Query Frame = 0

Query: 6   VDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDELN 65
           VDA YLKEIEK RRDLRALI    CAPIMLRLAWHDAGTYDAK KTGG +GSIRF +ELN
Sbjct: 5   VDAEYLKEIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELN 64

Query: 66  HTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVVPE 125
              NKGL+ AV FCE VKA+HP+++YADLYQLAGVVAVE+TGGP I F PGRKD+D   +
Sbjct: 65  RPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSA-D 124

Query: 126 EGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLKFDN 185
           +G LP+ ++GASHLR +F RMGL D+DIVALSGGHTLGRAHK+RSDFEGPWT+DPLKFDN
Sbjct: 125 DGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDN 184

Query: 186 SYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKLSEL 245
           SYFVELLK ++     LL+L TDKAL++DP+F  +VK YA DE  FF+ YA SHKKLSEL
Sbjct: 185 SYFVELLKGETPG---LLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSEL 244

Query: 246 GFTTPSTVLKSRASSWANKITVAAIVAVVAI 277
           GF  P  +  S  +     I VAA V +  I
Sbjct: 245 GFNPPRRI-PSAVTQQTLGIAVAAAVVIFTI 270

BLAST of Bhi03G000622 vs. TAIR 10
Match: AT3G09640.1 (ascorbate peroxidase 2 )

HSP 1 Score: 305.1 bits (780), Expect = 6.2e-83
Identity = 153/244 (62.70%), Postives = 177/244 (72.54%), Query Frame = 0

Query: 4   PKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDE 63
           P+V   Y K +++ +R LR LI ++ CAPI+LRLAWH AGT+D KTKTGGP G+IR   E
Sbjct: 7   PEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQE 66

Query: 64  LNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVV 123
           L H AN GL IAV   + +K   P ++YAD YQLAGVVAVEITGGP I F PGR D    
Sbjct: 67  LAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEP 126

Query: 124 PEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLKF 183
           P EGRLP   +G  HLRDVF RMGL DKDIVALSGGHTLGR HK+RS FEG WT +PL F
Sbjct: 127 PPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIF 186

Query: 184 DNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKLS 243
           DNSYF E+L   S  KE LL+LPTDKAL++DP F  +V+ YA DE  FFEDY  +H KLS
Sbjct: 187 DNSYFKEIL---SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLS 246

Query: 244 ELGF 248
           ELGF
Sbjct: 247 ELGF 247

BLAST of Bhi03G000622 vs. TAIR 10
Match: AT3G09640.2 (ascorbate peroxidase 2 )

HSP 1 Score: 305.1 bits (780), Expect = 6.2e-83
Identity = 153/244 (62.70%), Postives = 177/244 (72.54%), Query Frame = 0

Query: 4   PKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDE 63
           P+V   Y K +++ +R LR LI ++ CAPI+LRLAWH AGT+D KTKTGGP G+IR   E
Sbjct: 7   PEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQE 66

Query: 64  LNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVV 123
           L H AN GL IAV   + +K   P ++YAD YQLAGVVAVEITGGP I F PGR D    
Sbjct: 67  LAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEP 126

Query: 124 PEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLKF 183
           P EGRLP   +G  HLRDVF RMGL DKDIVALSGGHTLGR HK+RS FEG WT +PL F
Sbjct: 127 PPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIF 186

Query: 184 DNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKLS 243
           DNSYF E+L   S  KE LL+LPTDKAL++DP F  +V+ YA DE  FFEDY  +H KLS
Sbjct: 187 DNSYFKEIL---SGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLS 246

Query: 244 ELGF 248
           ELGF
Sbjct: 247 ELGF 247

BLAST of Bhi03G000622 vs. TAIR 10
Match: AT1G07890.3 (ascorbate peroxidase 1 )

HSP 1 Score: 288.9 bits (738), Expect = 4.6e-78
Identity = 146/245 (59.59%), Postives = 177/245 (72.24%), Query Frame = 0

Query: 4   PKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDE 63
           P V   Y K +EK RR LR LI ++ CAPIM+RLAWH AGT+D +++TGGP G++RF  E
Sbjct: 6   PTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTMRFDAE 65

Query: 64  LNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVV 123
             H AN G+ IA+   + ++ + P I++AD +QLAGVVAVE+TGGP I F PGR+D    
Sbjct: 66  QAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGREDKPQP 125

Query: 124 PEEGRLPDGHQGASHLRDVFYR-MGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLK 183
           P EGRLPD  +G  HLRDVF + MGL+DKDIVALSG HTLGR HKDRS FEG WT +PL 
Sbjct: 126 PPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWTSNPLI 185

Query: 184 FDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKL 243
           FDNSYF ELL   S  KE LL+L +DKAL++DP F   V+ YA DE  FF DYA +H KL
Sbjct: 186 FDNSYFKELL---SGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKL 245

Query: 244 SELGF 248
           SELGF
Sbjct: 246 SELGF 247

BLAST of Bhi03G000622 vs. ExPASy Swiss-Prot
Match: Q42564 (L-ascorbate peroxidase 3 OS=Arabidopsis thaliana OX=3702 GN=APX3 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 4.3e-105
Identity = 197/278 (70.86%), Postives = 221/278 (79.50%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MA P VDA YLKEI KARR+LR+LI  + CAPIMLRLAWHDAGTYDA++KTGGP+GSIR 
Sbjct: 1   MAAPIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRN 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
            +E  H AN GLKIA+D CE VKA+HPKITYADLYQLAGVVAVE+TGGP I FVPGRKDS
Sbjct: 61  EEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +V P+EGRLPD  QG  HLRDVFYRMGL+DKDIVALSGGHTLGRAH +RS F+GPWT++P
Sbjct: 121 NVCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLK +S   E LLKLPTDK L+EDP F + V+ YA DE  FF DYA SHK
Sbjct: 181 LKFDNSYFVELLKGES---EGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHK 240

Query: 241 KLSELGFTTPSTVLKSRASSW---ANKITVAAIVAVVA 276
           KLSELGF   S+  K+ A S     +   VA   AVVA
Sbjct: 241 KLSELGFNPNSSAGKAVADSTILAQSAFGVAVAAAVVA 275

BLAST of Bhi03G000622 vs. ExPASy Swiss-Prot
Match: Q01MI9 (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica OX=39946 GN=APX3 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 7.4e-105
Identity = 193/276 (69.93%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 2   APPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFM 61
           A P VDA Y+ E+E+ARRDLRALI  + CAPIMLRLAWHDAGTYD  TKTGGP+GSIRF 
Sbjct: 3   AAPVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFP 62

Query: 62  DELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSD 121
            E +H AN G+KIA+D  E +K RHPKITYADLYQLAGVVAVE+TGGPTID+VPGR+DS 
Sbjct: 63  QEYSHAANAGIKIAIDLLEPMKQRHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSS 122

Query: 122 VVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPL 181
             PEEGRLPD  +GA+HLR+VFYRMGL+DKDIVALSGGHTLG+A  +RS F+G WTKDPL
Sbjct: 123 DSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPL 182

Query: 182 KFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKK 241
           KFDNSYF+ELLK +S   E LLKLPTDKALVEDP F +YV+ YA DE  FF DYA SHKK
Sbjct: 183 KFDNSYFIELLKENS---EGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKK 242

Query: 242 LSELGFTTPST--VLKSRASSWANKITVAAIVAVVA 276
           LSELGFT P +  + KS     +  +  AA VAV A
Sbjct: 243 LSELGFTPPRSAFIYKSCQKPKSLLMQTAAGVAVAA 275

BLAST of Bhi03G000622 vs. ExPASy Swiss-Prot
Match: Q0JEQ2 (Probable L-ascorbate peroxidase 3, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=APX3 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.6e-104
Identity = 192/276 (69.57%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 2   APPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFM 61
           A P VDA Y+ E+E+ARRDLRALI  + CAPIMLRLAWHDAGTYD  TKTGGP+GSIRF 
Sbjct: 3   AAPVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFP 62

Query: 62  DELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSD 121
            E +H AN G+KIA+D  E +K +HPKITYADLYQLAGVVAVE+TGGPTID+VPGR+DS 
Sbjct: 63  QEYSHAANAGIKIAIDLLEPMKQKHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSS 122

Query: 122 VVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPL 181
             PEEGRLPD  +GA+HLR+VFYRMGL+DKDIVALSGGHTLG+A  +RS F+G WTKDPL
Sbjct: 123 DSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPL 182

Query: 182 KFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKK 241
           KFDNSYF+ELLK +S   E LLKLPTDKALVEDP F +YV+ YA DE  FF DYA SHKK
Sbjct: 183 KFDNSYFIELLKENS---EGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKK 242

Query: 242 LSELGFTTPST--VLKSRASSWANKITVAAIVAVVA 276
           LSELGFT P +  + KS     +  +  AA VAV A
Sbjct: 243 LSELGFTPPRSAFIYKSCQKPKSLLMQTAAGVAVAA 275

BLAST of Bhi03G000622 vs. ExPASy Swiss-Prot
Match: Q6ZJJ1 (Probable L-ascorbate peroxidase 4, peroxisomal OS=Oryza sativa subsp. japonica OX=39947 GN=APX4 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.3e-103
Identity = 188/285 (65.96%), Postives = 222/285 (77.89%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MA P VDA YL+++++ARR LRALI  + CAPIMLRLAWHDAGTYD  TKTGG +GSIR+
Sbjct: 1   MAAPVVDAEYLRQVDRARRHLRALISSKGCAPIMLRLAWHDAGTYDVNTKTGGANGSIRY 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
            +E  H +N GLKIA+D  E +KA+ PKITYADLYQLAGVVAVE+TGGPT++F+PGR+DS
Sbjct: 61  EEEYTHGSNAGLKIAIDLLEPIKAKSPKITYADLYQLAGVVAVEVTGGPTVEFIPGRRDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
            V P EGRLPD  +GA HLRD+FYRMGL+DKDIVALSGGHTLGRAH +RS FEG WT++P
Sbjct: 121 SVCPREGRLPDAKKGALHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFEGAWTQEP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYF+ELLK +S   E LLKLPTDKAL+EDP F +YV  YA DE  FF+DYA SHK
Sbjct: 181 LKFDNSYFLELLKGES---EGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVA------AIVAVVAILSY 280
           KLSELGFT  S+   S  S  +    +A      A+ A V I+SY
Sbjct: 241 KLSELGFTPRSSGPASTKSDLSTGAVLAQSAVGVAVAAAVVIVSY 282

BLAST of Bhi03G000622 vs. ExPASy Swiss-Prot
Match: Q7XZP5 (L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=APX5 PE=1 SV=2)

HSP 1 Score: 360.9 bits (925), Expect = 1.3e-98
Identity = 183/271 (67.53%), Postives = 212/271 (78.23%), Query Frame = 0

Query: 6   VDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRFMDELN 65
           VDA YLKEIEK RRDLRALI    CAPIMLRLAWHDAGTYDAK KTGG +GSIRF +ELN
Sbjct: 5   VDAEYLKEIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELN 64

Query: 66  HTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDSDVVPE 125
              NKGL+ AV FCE VKA+HP+++YADLYQLAGVVAVE+TGGP I F PGRKD+D   +
Sbjct: 65  RPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSA-D 124

Query: 126 EGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDPLKFDN 185
           +G LP+ ++GASHLR +F RMGL D+DIVALSGGHTLGRAHK+RSDFEGPWT+DPLKFDN
Sbjct: 125 DGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDN 184

Query: 186 SYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHKKLSEL 245
           SYFVELLK ++     LL+L TDKAL++DP+F  +VK YA DE  FF+ YA SHKKLSEL
Sbjct: 185 SYFVELLKGETPG---LLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSEL 244

Query: 246 GFTTPSTVLKSRASSWANKITVAAIVAVVAI 277
           GF  P  +  S  +     I VAA V +  I
Sbjct: 245 GFNPPRRI-PSAVTQQTLGIAVAAAVVIFTI 270

BLAST of Bhi03G000622 vs. NCBI nr
Match: XP_038881071.1 (L-ascorbate peroxidase 3 [Benincasa hispida])

HSP 1 Score: 593.6 bits (1529), Expect = 9.4e-166
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF
Sbjct: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS
Sbjct: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP
Sbjct: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGFLPNLF 294
           KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGFLPNLF
Sbjct: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGFLPNLF 293

BLAST of Bhi03G000622 vs. NCBI nr
Match: QCA42649.1 (ascorbate peroxidase [Citrullus lanatus])

HSP 1 Score: 544.7 bits (1402), Expect = 5.0e-151
Identity = 275/296 (92.91%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDA+TKTGGPDGSIRF
Sbjct: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAETKTGGPDGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +VVPEEGRLPD +QGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRS+FEGPWTKDP
Sbjct: 121 NVVPEEGRLPDANQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSNFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKNDSRSKE+LLKLPTDKALVEDPRF QYVKAYA DE KF EDYAASHK
Sbjct: 181 LKFDNSYFVELLKNDSRSKEQLLKLPTDKALVEDPRFSQYVKAYAKDEDKFLEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRA-SSWANKITVAAIVAVVAILSYKRAMSPE--GFLPNLF 294
           KLSELGFT PST LKS A SSWANKI VAAIVAVVAILSYKRAMSPE   FLP LF
Sbjct: 241 KLSELGFTKPSTGLKSSASSSWANKILVAAIVAVVAILSYKRAMSPEWDAFLPTLF 296

BLAST of Bhi03G000622 vs. NCBI nr
Match: XP_022950950.1 (L-ascorbate peroxidase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 515.8 bits (1327), Expect = 2.5e-142
Identity = 255/294 (86.73%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPPKVDAAYLKEIEKARRDLRALI KE CAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPKVDAAYLKEIEKARRDLRALIAKENCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M+ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVE+TGGP+IDFVPGRKDS
Sbjct: 61  MNELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEVTGGPSIDFVPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +VVPEEGRLPD +QGASHLRD+FYRMGLTDKDIVALSGGHTLGRAHKDRS+FEGPWTKDP
Sbjct: 121 NVVPEEGRLPDANQGASHLRDIFYRMGLTDKDIVALSGGHTLGRAHKDRSNFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLK  S S ++LLKLPTDKAL+ DPRF QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKKGSGSDQQLLKLPTDKALLADPRFSQYVKAYAEDEEKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGF-LPNLF 294
           KLSELGF +P TVL   ASSW N+I VA IVA VAILSYKR +SPEG   P LF
Sbjct: 241 KLSELGFVSPPTVLTDSASSWTNRIVVATIVAAVAILSYKRLISPEGVAFPTLF 294

BLAST of Bhi03G000622 vs. NCBI nr
Match: TYK12532.1 (L-ascorbate peroxidase 3, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 513.8 bits (1322), Expect = 9.5e-142
Identity = 258/295 (87.46%), Postives = 267/295 (90.51%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPP VDA YLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPIVDAVYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MKELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +   EEGRLPD HQGASHLRDVF RMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKD 
Sbjct: 121 NEAVEEGRLPDAHQGASHLRDVFCRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDF 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKN+SRS E+LLKLPTDKALV D +F QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKNNSRSGEQLLKLPTDKALVTDSQFSQYVKAYAKDEDKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEG--FLPNLF 294
           KLSELGFT PSTVL  R S+W N I VAAI+AVV ILSYKR M PEG  F PNLF
Sbjct: 241 KLSELGFTKPSTVLDGRGSAWTNGIVVAAIIAVVGILSYKRGMLPEGNAFFPNLF 295

BLAST of Bhi03G000622 vs. NCBI nr
Match: XP_016899164.1 (PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis melo])

HSP 1 Score: 512.3 bits (1318), Expect = 2.8e-141
Identity = 257/295 (87.12%), Postives = 267/295 (90.51%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPP VDA YLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPIVDAVYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MKELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +   EEGRLPD HQGASHLRDVF RMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKD 
Sbjct: 121 NEAVEEGRLPDAHQGASHLRDVFCRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDF 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKN+SRS E+LLKLPTDKALV D +F QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKNNSRSGEQLLKLPTDKALVTDSQFSQYVKAYAKDEDKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEG--FLPNLF 294
           KLSELGFT PS+VL  R S+W N I VAAI+AVV ILSYKR M PEG  F PNLF
Sbjct: 241 KLSELGFTKPSSVLDGRGSAWTNGIVVAAIIAVVGILSYKRGMLPEGNAFFPNLF 295

BLAST of Bhi03G000622 vs. ExPASy TrEMBL
Match: A0A4D6EWU8 (L-ascorbate peroxidase OS=Citrullus lanatus OX=3654 GN=CLAPX4 PE=2 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.4e-151
Identity = 275/296 (92.91%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDA+TKTGGPDGSIRF
Sbjct: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAETKTGGPDGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +VVPEEGRLPD +QGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRS+FEGPWTKDP
Sbjct: 121 NVVPEEGRLPDANQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSNFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKNDSRSKE+LLKLPTDKALVEDPRF QYVKAYA DE KF EDYAASHK
Sbjct: 181 LKFDNSYFVELLKNDSRSKEQLLKLPTDKALVEDPRFSQYVKAYAKDEDKFLEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRA-SSWANKITVAAIVAVVAILSYKRAMSPE--GFLPNLF 294
           KLSELGFT PST LKS A SSWANKI VAAIVAVVAILSYKRAMSPE   FLP LF
Sbjct: 241 KLSELGFTKPSTGLKSSASSSWANKILVAAIVAVVAILSYKRAMSPEWDAFLPTLF 296

BLAST of Bhi03G000622 vs. ExPASy TrEMBL
Match: A0A6J1GH77 (L-ascorbate peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111453891 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.2e-142
Identity = 255/294 (86.73%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPPKVDAAYLKEIEKARRDLRALI KE CAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPKVDAAYLKEIEKARRDLRALIAKENCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M+ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVE+TGGP+IDFVPGRKDS
Sbjct: 61  MNELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEVTGGPSIDFVPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +VVPEEGRLPD +QGASHLRD+FYRMGLTDKDIVALSGGHTLGRAHKDRS+FEGPWTKDP
Sbjct: 121 NVVPEEGRLPDANQGASHLRDIFYRMGLTDKDIVALSGGHTLGRAHKDRSNFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLK  S S ++LLKLPTDKAL+ DPRF QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKKGSGSDQQLLKLPTDKALLADPRFSQYVKAYAEDEEKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEGF-LPNLF 294
           KLSELGF +P TVL   ASSW N+I VA IVA VAILSYKR +SPEG   P LF
Sbjct: 241 KLSELGFVSPPTVLTDSASSWTNRIVVATIVAAVAILSYKRLISPEGVAFPTLF 294

BLAST of Bhi03G000622 vs. ExPASy TrEMBL
Match: A0A5D3CMH0 (L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001130 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.6e-142
Identity = 258/295 (87.46%), Postives = 267/295 (90.51%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPP VDA YLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPIVDAVYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MKELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +   EEGRLPD HQGASHLRDVF RMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKD 
Sbjct: 121 NEAVEEGRLPDAHQGASHLRDVFCRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDF 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKN+SRS E+LLKLPTDKALV D +F QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKNNSRSGEQLLKLPTDKALVTDSQFSQYVKAYAKDEDKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEG--FLPNLF 294
           KLSELGFT PSTVL  R S+W N I VAAI+AVV ILSYKR M PEG  F PNLF
Sbjct: 241 KLSELGFTKPSTVLDGRGSAWTNGIVVAAIIAVVGILSYKRGMLPEGNAFFPNLF 295

BLAST of Bhi03G000622 vs. ExPASy TrEMBL
Match: A0A1S4DT51 (L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103485146 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.3e-141
Identity = 257/295 (87.12%), Postives = 267/295 (90.51%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MAPP VDA YLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MAPPIVDAVYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
           M ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGP+IDFVPGRKD+
Sbjct: 61  MKELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPSIDFVPGRKDA 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +   EEGRLPD HQGASHLRDVF RMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKD 
Sbjct: 121 NEAVEEGRLPDAHQGASHLRDVFCRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDF 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLKN+SRS E+LLKLPTDKALV D +F QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKNNSRSGEQLLKLPTDKALVTDSQFSQYVKAYAKDEDKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEG--FLPNLF 294
           KLSELGFT PS+VL  R S+W N I VAAI+AVV ILSYKR M PEG  F PNLF
Sbjct: 241 KLSELGFTKPSSVLDGRGSAWTNGIVVAAIIAVVGILSYKRGMLPEGNAFFPNLF 295

BLAST of Bhi03G000622 vs. ExPASy TrEMBL
Match: A0A6J1IME4 (L-ascorbate peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111478732 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 6.2e-139
Identity = 248/287 (86.41%), Postives = 263/287 (91.64%), Query Frame = 0

Query: 1   MAPPKVDAAYLKEIEKARRDLRALIFKEKCAPIMLRLAWHDAGTYDAKTKTGGPDGSIRF 60
           MA PKVDAAYLKEIEKARRDLRALI KE CAPIMLRLAWHDAGTYDAKTKTGGP+GSIRF
Sbjct: 1   MALPKVDAAYLKEIEKARRDLRALIAKENCAPIMLRLAWHDAGTYDAKTKTGGPNGSIRF 60

Query: 61  MDELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEITGGPTIDFVPGRKDS 120
            +ELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVE+TGGP+IDF PGRKDS
Sbjct: 61  KNELNHTANKGLKIAVDFCETVKARHPKITYADLYQLAGVVAVEVTGGPSIDFAPGRKDS 120

Query: 121 DVVPEEGRLPDGHQGASHLRDVFYRMGLTDKDIVALSGGHTLGRAHKDRSDFEGPWTKDP 180
           +VVPEEGRLPD +QGASHLRD+FYRMGLTDKDIVALSGGHTLGRAHKDRS+FEGPWTKDP
Sbjct: 121 NVVPEEGRLPDANQGASHLRDIFYRMGLTDKDIVALSGGHTLGRAHKDRSNFEGPWTKDP 180

Query: 181 LKFDNSYFVELLKNDSRSKEKLLKLPTDKALVEDPRFFQYVKAYANDEVKFFEDYAASHK 240
           LKFDNSYFVELLK  S S ++LLKLPTDKAL+ DPRF QYVKAYA DE KFFEDYAASHK
Sbjct: 181 LKFDNSYFVELLKKGSGSDQQLLKLPTDKALLADPRFSQYVKAYAEDEEKFFEDYAASHK 240

Query: 241 KLSELGFTTPSTVLKSRASSWANKITVAAIVAVVAILSYKRAMSPEG 288
           KLSELGF +P T LK  ASSW ++I VA IVA VAILSYKR +SPEG
Sbjct: 241 KLSELGFRSPLTDLKDSASSWTSRIVVATIVAAVAILSYKRLVSPEG 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G35000.13.1e-10670.86ascorbate peroxidase 3 [more]
AT4G35970.19.6e-10067.53ascorbate peroxidase 5 [more]
AT3G09640.16.2e-8362.70ascorbate peroxidase 2 [more]
AT3G09640.26.2e-8362.70ascorbate peroxidase 2 [more]
AT1G07890.34.6e-7859.59ascorbate peroxidase 1 [more]
Match NameE-valueIdentityDescription
Q425644.3e-10570.86L-ascorbate peroxidase 3 OS=Arabidopsis thaliana OX=3702 GN=APX3 PE=1 SV=1[more]
Q01MI97.4e-10569.93Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica OX=39946 GN=APX3... [more]
Q0JEQ21.6e-10469.57Probable L-ascorbate peroxidase 3, peroxisomal OS=Oryza sativa subsp. japonica O... [more]
Q6ZJJ15.3e-10365.96Probable L-ascorbate peroxidase 4, peroxisomal OS=Oryza sativa subsp. japonica O... [more]
Q7XZP51.3e-9867.53L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=APX5 PE... [more]
Match NameE-valueIdentityDescription
XP_038881071.19.4e-166100.00L-ascorbate peroxidase 3 [Benincasa hispida][more]
QCA42649.15.0e-15192.91ascorbate peroxidase [Citrullus lanatus][more]
XP_022950950.12.5e-14286.73L-ascorbate peroxidase 3 isoform X1 [Cucurbita moschata][more]
TYK12532.19.5e-14287.46L-ascorbate peroxidase 3, peroxisomal [Cucumis melo var. makuwa][more]
XP_016899164.12.8e-14187.12PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A4D6EWU82.4e-15192.91L-ascorbate peroxidase OS=Citrullus lanatus OX=3654 GN=CLAPX4 PE=2 SV=1[more]
A0A6J1GH771.2e-14286.73L-ascorbate peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111453891 PE=3 SV=1[more]
A0A5D3CMH04.6e-14287.46L-ascorbate peroxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A1S4DT511.3e-14187.12L-ascorbate peroxidase OS=Cucumis melo OX=3656 GN=LOC103485146 PE=3 SV=1[more]
A0A6J1IME46.2e-13986.41L-ascorbate peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111478732 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002016Haem peroxidasePRINTSPR00458PEROXIDASEcoord: 176..191
score: 43.89
coord: 31..45
score: 45.53
coord: 107..119
score: 48.52
coord: 152..167
score: 56.5
coord: 89..106
score: 36.38
IPR002016Haem peroxidasePFAMPF00141peroxidasecoord: 18..227
e-value: 2.3E-48
score: 164.9
IPR002016Haem peroxidasePROSITEPS50873PEROXIDASE_4coord: 89..265
score: 16.665783
IPR002207Class I peroxidasePRINTSPR00459ASPEROXIDASEcoord: 49..59
score: 65.03
coord: 10..30
score: 53.48
coord: 63..87
score: 51.23
coord: 128..145
score: 58.97
coord: 31..46
score: 83.65
coord: 146..167
score: 86.01
coord: 168..192
score: 74.31
coord: 223..247
score: 67.85
coord: 88..106
score: 75.71
coord: 107..119
score: 69.53
NoneNo IPR availableGENE3D1.10.420.10Peroxidase, domain 2coord: 130..239
e-value: 3.6E-104
score: 349.0
NoneNo IPR availableGENE3D1.10.520.10coord: 17..246
e-value: 3.6E-104
score: 349.0
NoneNo IPR availablePIRSRPIRSR600823-3PIRSR600823-3coord: 30..166
e-value: 4.8E-14
score: 49.8
NoneNo IPR availablePIRSRPIRSR601621-2PIRSR601621-2coord: 21..249
e-value: 2.3E-18
score: 64.1
NoneNo IPR availablePANTHERPTHR31356:SF38L-ASCORBATE PEROXIDASE 5, PEROXISOMALcoord: 2..280
NoneNo IPR availableCDDcd00691ascorbate_peroxidasecoord: 4..248
e-value: 1.26553E-152
score: 425.079
IPR044831Heme-binding peroxidase Ccp1-likePANTHERPTHR31356THYLAKOID LUMENAL 29 KDA PROTEIN, CHLOROPLASTIC-RELATEDcoord: 2..280
IPR019794Peroxidase, active sitePROSITEPS00436PEROXIDASE_2coord: 31..42
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 152..162
IPR010255Haem peroxidase superfamilySUPERFAMILY48113Heme-dependent peroxidasescoord: 12..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000622Bhi03M000622mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0034599 cellular response to oxidative stress
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0016688 L-ascorbate peroxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004601 peroxidase activity