Bhi03G000581 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000581
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	endochitinase-like
Location	chr3: 14545794 .. 14547545 (+)
RNA-Seq Expression	Bhi03G000581
Synteny	Bhi03G000581

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCATTTCTCTCATATATTACATCAATGAAAAATATGAAGGCCTATTCACTTATAATTTTAGTCTCTGTCTTTGATTTGGGAGCTGTCTCAGCCGAGCAATGTGGGCGGCAGGCCAATGGCGCCTTGTGCCCCAATGGTCTCTGTTGTAGCCAGTTCGGGTTCTGTGGCAATACCGATGACTACTGTAAAACTGGTTGCCAGAGCCAGTGTCGCGGCTCAAGTTCTCCTACACCCTCTGGTAGTGGCGTTGGAAGTATCATCAGTGAGAGTCTTTACAATCAAATGCTCAAGTATAGCAGGGATCCTCGGTGTCCTAGTAATGGCTTCTATGCCTATAATGCTTTTATTACTGCTGCTAACTCCTTCCCAGGCTTTGGGACCACGGGAGATGTTGCTACTCGGAAAAGGGAGCTTGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGTTTGTTTGAAAACTATATATTTATTCTTTTAAAAGTTGCAATGTTACTCTTAACTTAAAAACAATTTGTGAGATGCAGGAGGATGGCCAACTGCACCGGATGGCCCATATGCGTGGGGATACTGTTTTATACGCGAGAGAAATCAAGATGTATATTGCACACCTAGTCAACAATGGCCATGTGCTGCTGGTCAAAAATATTACGGTCGTGGACCGATCCAACTAACTCAGTAAGATTTTTTTTGGGTTATATCATTTTGATTTCAATACTTTAGAGTTTGGTCAATTTTAGTCTTTGAACTTTAGATAAATCTTAAAATTTAGTCCATCAATTTTAAAATTTATCAAAATTGGATAAATGATAACAATAATTTTCATACAACAAAATACAATATGTTTTCAAAAGTTATAGTAAAAGAATTTTTATAGAAAAAGTTCTCAAAAAAAACAAATGAGAAACTAGTTGTAAGGAATAAATTTAAGATTTATCGAAGGTACACAAAGTAAATTGGACATTTAAAAGTTGATGAACTAAAGTTAAACAAACTTTAAAGTATATAGACCAAAATAGTATCTTTAATATTAAATCTAATCACGAGTTATTATCTGAACCATAATTCATATCATTTTGAATGTTTTTTAGCAACTACAACTACGGGCCAGCAGGGAATGCAATAAATTCGAATTTGCTGAGCAACCCTGATTTAGTCGCCACAAATGCAGTTATATCTTTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCTTGTCATGATGTTATTACTGGTAGATGGCAGCCTTCAAGTGCTGACACAGCTGCAGGAAGAGTCCCTGGATATGGTGTTCTAACCAACATCATTAATGGTGGACTCGAGTGTGGGCGTGGCCCAGATGGTAGAGTTGCTGATAGGATTGGATTTTTCAAACGATATTGCGACATGTTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTTTAAATATTTAGTAATCTTATATAGTATGCAATAAAGTGTCCTCAAAGATGCAGAGAAAGTACGAGGGCTGGTATGTGTAACATCTAAACGTATTTAATAATGTTACTTTCAAGTTTCAATAAAGTGAGGATCTTCACTTTTCCCATATATCAATGGGACTTCATCTCTCTTTTCTTTTAGGTTTTAAATACATATACTAGGTTAAAAAATATTTTTGGTCCCTAAACTTCCATAAAAGTAATAATTTAGTTCTTAAATTTTGGTTGGTAACAATTTAATCCTTATACTTTCA

mRNA sequence

CCTCATTTCTCTCATATATTACATCAATGAAAAATATGAAGGCCTATTCACTTATAATTTTAGTCTCTGTCTTTGATTTGGGAGCTGTCTCAGCCGAGCAATGTGGGCGGCAGGCCAATGGCGCCTTGTGCCCCAATGGTCTCTGTTGTAGCCAGTTCGGGTTCTGTGGCAATACCGATGACTACTGTAAAACTGGTTGCCAGAGCCAGTGTCGCGGCTCAAGTTCTCCTACACCCTCTGGTAGTGGCGTTGGAAGTATCATCAGTGAGAGTCTTTACAATCAAATGCTCAAGTATAGCAGGGATCCTCGGTGTCCTAGTAATGGCTTCTATGCCTATAATGCTTTTATTACTGCTGCTAACTCCTTCCCAGGCTTTGGGACCACGGGAGATGTTGCTACTCGGAAAAGGGAGCTTGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGAGGATGGCCAACTGCACCGGATGGCCCATATGCGTGGGGATACTGTTTTATACGCGAGAGAAATCAAGATGTATATTGCACACCTAGTCAACAATGGCCATGTGCTGCTGGTCAAAAATATTACGGTCGTGGACCGATCCAACTAACTCACAACTACAACTACGGGCCAGCAGGGAATGCAATAAATTCGAATTTGCTGAGCAACCCTGATTTAGTCGCCACAAATGCAGTTATATCTTTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCTTGTCATGATGTTATTACTGGTAGATGGCAGCCTTCAAGTGCTGACACAGCTGCAGGAAGAGTCCCTGGATATGGTGTTCTAACCAACATCATTAATGGTGGACTCGAGTGTGGGCGTGGCCCAGATGGTAGAGTTGCTGATAGGATTGGATTTTTCAAACGATATTGCGACATGTTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTTTAAATATTTAGTAATCTTATATAGTATGCAATAAAGTGTCCTCAAAGATGCAGAGAAAGTACGAGGGCTGGTATGTGTAACATCTAAACGTATTTAATAATGTTACTTTCAAGTTTCAATAAAGTGAGGATCTTCACTTTTCCCATATATCAATGGGACTTCATCTCTCTTTTCTTTTAGGTTTTAAATACATATACTAGGTTAAAAAATATTTTTGGTCCCTAAACTTCCATAAAAGTAATAATTTAGTTCTTAAATTTTGGTTGGTAACAATTTAATCCTTATACTTTCA

Coding sequence (CDS)

ATGAAAAATATGAAGGCCTATTCACTTATAATTTTAGTCTCTGTCTTTGATTTGGGAGCTGTCTCAGCCGAGCAATGTGGGCGGCAGGCCAATGGCGCCTTGTGCCCCAATGGTCTCTGTTGTAGCCAGTTCGGGTTCTGTGGCAATACCGATGACTACTGTAAAACTGGTTGCCAGAGCCAGTGTCGCGGCTCAAGTTCTCCTACACCCTCTGGTAGTGGCGTTGGAAGTATCATCAGTGAGAGTCTTTACAATCAAATGCTCAAGTATAGCAGGGATCCTCGGTGTCCTAGTAATGGCTTCTATGCCTATAATGCTTTTATTACTGCTGCTAACTCCTTCCCAGGCTTTGGGACCACGGGAGATGTTGCTACTCGGAAAAGGGAGCTTGCAGCTTTCTTTGGCCAAACTTCTCATGAGACTACTGGAGGATGGCCAACTGCACCGGATGGCCCATATGCGTGGGGATACTGTTTTATACGCGAGAGAAATCAAGATGTATATTGCACACCTAGTCAACAATGGCCATGTGCTGCTGGTCAAAAATATTACGGTCGTGGACCGATCCAACTAACTCACAACTACAACTACGGGCCAGCAGGGAATGCAATAAATTCGAATTTGCTGAGCAACCCTGATTTAGTCGCCACAAATGCAGTTATATCTTTCAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCTTGTCATGATGTTATTACTGGTAGATGGCAGCCTTCAAGTGCTGACACAGCTGCAGGAAGAGTCCCTGGATATGGTGTTCTAACCAACATCATTAATGGTGGACTCGAGTGTGGGCGTGGCCCAGATGGTAGAGTTGCTGATAGGATTGGATTTTTCAAACGATATTGCGACATGTTGGGTATTGGTTATGGCAACAACTTAGATTGCTACAATCAAAGGTCTTTTTAA

Protein sequence

MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCRGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYGNNLDCYNQRSF

Homology

[Close]

BLAST of Bhi03G000581 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 463.0 bits (1190), Expect = 1.9e-130
Identity = 215/317 (67.82%), Postives = 250/317 (78.86%), Query Frame = 0


Query: 1   MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCK-TGCQ 60
           +  MK    + L+    L   SAEQCGRQA GALCPNGLCCS+FG+CGNT+ YCK  GCQ
Sbjct: 11  LNKMKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQ 70

Query: 61  SQCR-GSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFG 120
           SQC  G + P P+G  +  IIS S ++ MLK+  D  CP+ GFY YNAFITAA SFPGFG
Sbjct: 71  SQCTPGGTPPGPTGD-LSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFG 130

Query: 121 TTGDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QDVYCTPSQQWPC 180
           TTGD ATRK+E+AAFFGQTSHETTGGW TAPDGPY+WGYCF +E+N    YC PS  WPC
Sbjct: 131 TTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPC 190

Query: 181 AAGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNK 240
           A+G++YYGRGP+QL+ NYNYG  G AI  +LL+NPDLVA +AVI+FK AIWFWMT Q  K
Sbjct: 191 ASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPK 250

Query: 241 PSCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDM 300
           PSCH VI G+WQPS AD AAGR+PGYGV+TNIINGGLECGRG DGRVADRIGF++RYC++
Sbjct: 251 PSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNI 310

Query: 301 LGIGYGNNLDCYNQRSF 315
            G+  G NLDCYNQRSF
Sbjct: 311 FGVNPGGNLDCYNQRSF 326

[Close]

BLAST of Bhi03G000581 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.1e-81
Identity = 136/240 (56.67%), Postives = 173/240 (72.08%), Query Frame = 0


Query: 77  SIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVATRKRELAAFFGQ 136
           S++  +LY+Q+  +  +  CP+ GFY Y AF+ A  SFP FG+ G+  TR+RE+AAF  Q
Sbjct: 40  SLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQ 99

Query: 137 TSHETTGGWPTAPDGPYAWGYCFIRERN-QDVYCTPS-QQWPCAAGQKYYGRGPIQLTHN 196
            SHETTGGW TAPDGPYAWG CF  E + Q  YC  S + WPC +G+ Y GRGPIQL+ N
Sbjct: 100 ISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWN 159

Query: 197 YNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDVITGRWQPSSAD 256
           YNYG AG A+  + L NP+LVA N+V++FKTA+WFWMT Q  KPSCH+V+  R++P+ AD
Sbjct: 160 YNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKAD 219

Query: 257 TAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYGNNLDCYNQRSF 315
            AA R  GYG++TNIINGGLECG   DGRV DR+G+F+RY  +  +  G NLDC NQR F
Sbjct: 220 RAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

[Close]

BLAST of Bhi03G000581 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-80
Identity = 135/251 (53.78%), Postives = 173/251 (68.92%), Query Frame = 0


Query: 66  SSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVAT 125
           SS     + +  ++   LYN++  +  +  CP+NGFY Y +F+ A   FP FG+ G   T
Sbjct: 21  SSAQTEATSIERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVT 80

Query: 126 RKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QDVYCTPSQ-QWPCAAGQKY 185
           ++ E+AAF  Q SHETTGGW TAPDGPYAWG CF  E + Q  YC  S  QWPC   + Y
Sbjct: 81  QRLEVAAFLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTY 140

Query: 186 YGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDV 245
            GRGPIQL+ NYNYGPAG A+  + L NP+ V+ N+VI+F+TA+WFWMTPQ  KPSCHDV
Sbjct: 141 QGRGPIQLSWNYNYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDV 200

Query: 246 ITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYG 305
           + G+++P++AD AA R  G+G+ TNIINGGLECG   DGRV DRIGFF+RY  +  +  G
Sbjct: 201 MIGKYRPTAADLAANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATG 260

Query: 306 NNLDCYNQRSF 315
            NLDC NQR +
Sbjct: 261 PNLDCENQRPY 271

[Close]

BLAST of Bhi03G000581 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 195.3 bits (495), Expect = 7.4e-50
Identity = 112/310 (36.13%), Postives = 159/310 (51.29%), Query Frame = 0


Query: 2   KNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQ 61
           K++   ++++++  F      A+ CG       C + LCCSQFGFCGNT DYC  GCQ Q
Sbjct: 8   KSISLVTILLVLQAFS-NTTKAQNCG-------CSSELCCSQFGFCGNTSDYCGVGCQ-Q 67

Query: 62  CRGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTG 121
               + P  +G  V  I+++  +N ++  +    C  N FY+  AF+ A +S+  FG  G
Sbjct: 68  GPCFAPPPANGVSVAEIVTQEFFNGIISQAAS-SCAGNRFYSRGAFLEALDSYSRFGRVG 127

Query: 122 DVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRE---RNQDVYCTPSQQWPCA 181
                +RE+AAFF   +HET               +C+I E    ++D     + Q+PC 
Sbjct: 128 STDDSRREIAAFFAHVTHET-------------GHFCYIEEIDGASKDYCDENATQYPCN 187

Query: 182 AGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKP 241
             + YYGRGPIQL+ N+NYGPAG AI  + L+ P+ VAT+ VISFKTA+W+W        
Sbjct: 188 PNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATDPVISFKTALWYW-------- 247

Query: 242 SCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDML 301
                 T R QP  +        G+G     ING LEC       V  R+ ++  YC  L
Sbjct: 248 ------TNRVQPVISQ-------GFGATIRAINGALECDGANTATVQARVRYYTDYCRQL 273

Query: 302 GIGYGNNLDC 309
           G+  GNNL C
Sbjct: 308 GVDPGNNLTC 273

[Close]

BLAST of Bhi03G000581 vs. TAIR 10
Match: AT2G43590.1 (Chitinase family protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.7e-49
Identity = 113/307 (36.81%), Postives = 160/307 (52.12%), Query Frame = 0


Query: 8   SLIILVSV--FDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQS-QCRG 67
           SL++L+ +  F    V ++ CG       C   LCCSQFG+CG  D YC  GC+S  CRG
Sbjct: 7   SLVLLLCLLGFFSETVKSQNCG-------CAPNLCCSQFGYCGTDDAYCGVGCRSGPCRG 66

Query: 68  SSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVA 127
           S   TP+G  VGSI+++  +N ++  + +  C    FY  ++F+ AAN+FP F      +
Sbjct: 67  SG--TPTGGSVGSIVTQGFFNNIINQAGN-GCAGKRFYTRDSFVNAANTFPNFAN----S 126

Query: 128 TRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDV--YCTPSQ-QWPCAAGQ 187
             +RE+A  F   +HET               +C+I E N     YC  S  Q+PCA G+
Sbjct: 127 VTRREIATMFAHFTHET-------------GHFCYIEEINGATRNYCQSSNTQYPCAPGK 186

Query: 188 KYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCH 247
            Y+GRGPIQL+ NYNYG  G ++  +LL  P+LV +N  ++F+T +WFWM       S  
Sbjct: 187 GYFGRGPIQLSWNYNYGACGQSLGLDLLRQPELVGSNPTVAFRTGLWFWMN------SVR 246

Query: 248 DVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIG 307
            V+                 G+G     IN G+EC  G  G V  RIG+++ YC  LG+ 
Sbjct: 247 PVLN---------------QGFGATIRAIN-GMECNGGNSGAVNARIGYYRDYCGQLGVD 264

Query: 308 YGNNLDC 309
            G NL C
Sbjct: 307 PGPNLSC 264

[Close]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot
Match: Q42993 (Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 6.9e-133
Identity = 216/321 (67.29%), Postives = 253/321 (78.82%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCR 63
           M+A +++++ + F + AV  EQCG QA GALCPN LCCSQ+G+CG+T  YC +GCQSQC 
Sbjct: 1   MRALAVVVVATAFAVVAVRGEQCGSQAGGALCPNCLCCSQYGWCGSTSAYCGSGCQSQCS 60

Query: 64  GS---SSPTP----SGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPG 123
           GS     PTP     GSGV SI+S SL++QML +  D  CP+  FY Y+AF+ AAN+FP 
Sbjct: 61  GSCGGGGPTPPSGGGGSGVASIVSRSLFDQMLLHRNDAACPAKNFYTYDAFVAAANAFPS 120

Query: 124 FGTTGDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDV---YCTPSQ 183
           F TTGD ATRKRE+AAF  QTSHETTGGW TAPDGPY+WGYCF  E N +V   YC  S 
Sbjct: 121 FATTGDAATRKREVAAFLAQTSHETTGGWATAPDGPYSWGYCFKEENNGNVGSDYCVQSS 180

Query: 184 QWPCAAGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTP 243
           QWPCAAG+KYYGRGPIQ+++NYNYGPAG AI SNLLSNPDLVA++A +SFKTA WFWMTP
Sbjct: 181 QWPCAAGKKYYGRGPIQISYNYNYGPAGQAIGSNLLSNPDLVASDATVSFKTAFWFWMTP 240

Query: 244 QGNKPSCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKR 303
           Q  KPSCH V+TG+W P+  D AAGRVPGYGV+TNIINGG+ECG G D RVADRIGF+KR
Sbjct: 241 QSPKPSCHAVMTGQWTPNGNDQAAGRVPGYGVVTNIINGGVECGHGADSRVADRIGFYKR 300

Query: 304 YCDMLGIGYGNNLDCYNQRSF 315
           YCDMLG+ YG NLDCYNQR F
Sbjct: 301 YCDMLGVSYGANLDCYNQRPF 321

[Close]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 472.6 bits (1215), Expect = 3.4e-132
Identity = 214/307 (69.71%), Postives = 254/307 (82.74%), Query Frame = 0


Query: 9   LIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCRGSSSP 68
           L++LV +   G   AEQCGRQA GALCP GLCCSQFG+CG+T DYC   CQSQC G  +P
Sbjct: 12  LLLLVGLL-AGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQC-GGVTP 71

Query: 69  TPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVATRKR 128
           +P G GV S+IS+S++NQMLK+  D  C + GFY YNAFI AANSF GF + GD ATRKR
Sbjct: 72  SP-GGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTATRKR 131

Query: 129 ELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRER-NQDVYCTPSQQWPCAAGQKYYGRG 188
           E+AAF  QTSHETTGGW TAPDGPYAWGYCF++E+ N   YC P+ QWPCA G+KYYGRG
Sbjct: 132 EIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYYGRG 191

Query: 189 PIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDVITGR 248
           PIQ+++NYNYGPAG AI  +L++NPD VAT+ VISFKTA+WFWMTPQ  KPSCH+VITGR
Sbjct: 192 PIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCHNVITGR 251

Query: 249 WQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYGNNLD 308
           W PS+AD AAGR+PGYGV+TNIINGG+ECG+G + +VADRIGF+KRYCD+LG+ YG+NLD
Sbjct: 252 WTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVSYGSNLD 311

Query: 309 CYNQRSF 315
           CYNQRSF
Sbjct: 312 CYNQRSF 315

[Close]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot
Match: P36907 (Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.4e-130
Identity = 214/319 (67.08%), Postives = 253/319 (79.31%), Query Frame = 0


Query: 2   KNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQ 61
           + +K    I+ +    LG+  AEQCG QA GA+CPNGLCCS+FGFCG+TD YC  GCQSQ
Sbjct: 3   RTLKVSFFILCLLPLFLGS-KAEQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQSQ 62

Query: 62  CRGSSS---PTPS--GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPG 121
           C+ S +   PTPS  G  VG ++  SL++QMLKY  D RC  +GFY Y+AFI AA SF G
Sbjct: 63  CKSSPTPTIPTPSTGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIAAARSFNG 122

Query: 122 FGTTGDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QDVYCTPSQQW 181
           FGTTGD  T+K+ELAAF  QTSHETTGGWPTAPDGPYAWGYCF+ E+N Q+VYC+P + W
Sbjct: 123 FGTTGDDNTKKKELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSP-KDW 182

Query: 182 PCAAGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQG 241
           PCA G+KYYGRGPIQLTHNYNYG AG AI  +L++NPDL++TN  +SFKTAIWFWMTPQ 
Sbjct: 183 PCAPGKKYYGRGPIQLTHNYNYGLAGQAIKEDLINNPDLLSTNPTVSFKTAIWFWMTPQA 242

Query: 242 NKPSCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYC 301
           NKPS HDVITGRW PS+AD++AGRVPGYGV+TNIINGG+ECG G D RV DR+GF+KRYC
Sbjct: 243 NKPSSHDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYKRYC 302

Query: 302 DMLGIGYGNNLDCYNQRSF 315
            + G+  G NLDC NQRSF
Sbjct: 303 QIFGVDPGGNLDCNNQRSF 319

[Close]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-129
Identity = 214/320 (66.88%), Postives = 253/320 (79.06%), Query Frame = 0


Query: 1   MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTG-CQ 60
           M+  K  +L  L+    L + SAEQCG QA GA CP+GLCCS+FG+CGNT+DYC  G CQ
Sbjct: 1   MRLCKFTALSSLLFSLLLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQ 60

Query: 61  SQCRGSSSPT----PSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFP 120
           SQC G  +PT    P G  +GSIIS S+++QMLK+  D  C   GFY+YNAFI AA SFP
Sbjct: 61  SQCPGGPTPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFP 120

Query: 121 GFGTTGDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRER-NQDVYCTPSQQ 180
           GFGT+GD   RKRE+AAFF QTSHETTGGW TAPDGPYAWGYC++RE+ +   YCTPS Q
Sbjct: 121 GFGTSGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQ 180

Query: 181 WPCAAGQKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQ 240
           WPCA G+KY+GRGPIQ++HNYNYGP G AI  +LL+NPDLVAT+ VISFK+A+WFWMTPQ
Sbjct: 181 WPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQ 240

Query: 241 GNKPSCHDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRY 300
             KPSCHDVI GRWQPS+ D AA R+PG+GV+TNIINGGLECGRG D RV DRIGF++RY
Sbjct: 241 SPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRY 300

Query: 301 CDMLGIGYGNNLDCYNQRSF 315
           C +LG+  G+NLDC NQRSF
Sbjct: 301 CSILGVSPGDNLDCGNQRSF 320

[Close]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-129
Identity = 214/313 (68.37%), Postives = 252/313 (80.51%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCK-TGCQSQC 63
           MK+  L+ L+  F L    AEQCGRQA GALCPNGLCCS+FG+CG+T+ YCK  GCQSQC
Sbjct: 1   MKSCLLLFLIFSFLLSFSLAEQCGRQAGGALCPNGLCCSEFGWCGDTEAYCKQPGCQSQC 60

Query: 64  RGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGD 123
            G + P P+G  +  IIS S ++ MLK+  D  CP+ GFY Y+AFI AA SFPGFGTTGD
Sbjct: 61  -GGTPPGPTGD-LSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGD 120

Query: 124 VATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERN-QDVYCTPSQQWPCAAGQ 183
            ATRK+E+AAFFGQTSHETTGGW TAPDGPY+WGYCF +E+N    YC+PS +WPCA+G+
Sbjct: 121 TATRKKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGK 180

Query: 184 KYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCH 243
            YYGRGP+QL+ NYNYG  G AI S+LL+NPDLV+ + VI+FK AIWFWMTPQ  KPSCH
Sbjct: 181 SYYGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCH 240

Query: 244 DVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIG 303
            VI G+WQPS AD AAGRVPGYGV+TNIINGGLECGRG D RVADRIGF++RYC++LG+ 
Sbjct: 241 AVIVGQWQPSDADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVN 300

Query: 304 YGNNLDCYNQRSF 315
            G NLDCYNQRSF
Sbjct: 301 PGGNLDCYNQRSF 311

[Close]

BLAST of Bhi03G000581 vs. NCBI nr
Match: XP_038881302.1 (endochitinase-like [Benincasa hispida])

HSP 1 Score: 677.6 bits (1747), Expect = 5.3e-191
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQS 60
           MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQS
Sbjct: 1   MKNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQS 60

Query: 61  QCRGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 120
           QCRGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT
Sbjct: 61  QCRGSSSPTPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 120

Query: 121 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 180
           GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG
Sbjct: 121 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 180

Query: 181 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 240
           QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC
Sbjct: 181 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 240

Query: 241 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 300
           HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI
Sbjct: 241 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 300

Query: 301 GYGNNLDCYNQRSF 315
           GYGNNLDCYNQRSF
Sbjct: 301 GYGNNLDCYNQRSF 314

[Close]

BLAST of Bhi03G000581 vs. NCBI nr
Match: XP_004134833.1 (endochitinase [Cucumis sativus])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 269/314 (85.67%), Postives = 285/314 (90.76%), Query Frame = 0


Query: 2   KNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQ 61
           KNMK YSL+IL   F L  V+AEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQ
Sbjct: 7   KNMKTYSLVILCFAFLLVVVAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQ 66

Query: 62  CRGSSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 121
           CRGSS+PTPS GSGVGSIISESLYNQMLKYSRD RCPSNGFY YNAFITAA  FP FG T
Sbjct: 67  CRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYNAFITAARFFPAFGNT 126

Query: 122 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 181
           G V TRKRE+AAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA+G
Sbjct: 127 GSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQAYCTPSQQWPCASG 186

Query: 182 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 241
           Q+YYGRGPIQLTHNYNYGPAGNAI + LL++PDLVAT+AV+SFKTA+WFWMT QGNKPSC
Sbjct: 187 QQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSC 246

Query: 242 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 301
           H+VITG WQPSSAD AAGR PGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGI
Sbjct: 247 HNVITGNWQPSSADNAAGRSPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGI 306

Query: 302 GYGNNLDCYNQRSF 315
           GYGNNLDCYNQRSF
Sbjct: 307 GYGNNLDCYNQRSF 320

[Close]

BLAST of Bhi03G000581 vs. NCBI nr
Match: KAA0025620.1 (family 19 chitinase [Cucumis melo var. makuwa] >TYK12495.1 family 19 chitinase [Cucumis melo var. makuwa])

HSP 1 Score: 586.3 bits (1510), Expect = 1.6e-163
Identity = 269/312 (86.22%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCR 63
           MK YSL+IL   F LGA SAEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQCR
Sbjct: 1   MKTYSLVILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 64  GSSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGD 123
           GSS+P PS GSGVGSIISESLYNQMLKYSRDPRCPSNGFY YNAFITAA SFP FGTTGD
Sbjct: 61  GSSTPPPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 124 VATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQK 183
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA GQ+
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 184 YYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHD 243
           YYGRGPIQLTHNYNYGPAG AI + LL+NPD VAT+ V SFKTA+WFWMT QGNKPSCH+
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 244 VITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGY 303
           VITG WQPSSAD AAGRVPGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 304 GNNLDCYNQRSF 315
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 312

[Close]

BLAST of Bhi03G000581 vs. NCBI nr
Match: XP_022949791.1 (endochitinase-like [Cucurbita moschata])

HSP 1 Score: 584.3 bits (1505), Expect = 6.1e-163
Identity = 268/314 (85.35%), Postives = 288/314 (91.72%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCR 63
           MK ++LI+L   F LG  +AEQCGRQANGALCPNGLCCSQFG+CG+TD+YCK GCQSQC 
Sbjct: 8   MKLHTLIVLAFAFVLGG-AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 64  GSSSPTP---SGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 123
           GS+  TP    GSGVGSIISESLYNQMLKYS+DPRCPSNGFY YNAF+TAA SFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 124 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 183
           GD ATRKRELAAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQDVYC+P+QQWPCAAG
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 184 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 243
           QKYYGRGPIQLTHNYNYGPAGNA+N NLL NPDLVAT+AV+SFKTAIWFWMTPQGNKPSC
Sbjct: 188 QKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 247

Query: 244 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 303
           HDVITGRWQPSSAD+AAGRVPGYGV+TNIINGGLECG GPD RVADRIGF+KRY DMLG+
Sbjct: 248 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 307

Query: 304 GYGNNLDCYNQRSF 315
            YG+NLDC NQRSF
Sbjct: 308 SYGSNLDCNNQRSF 320

[Close]

BLAST of Bhi03G000581 vs. NCBI nr
Match: APF47214.1 (family 19 chitinase, partial [Cucumis melo var. saccharinus])

HSP 1 Score: 581.6 bits (1498), Expect = 4.0e-162
Identity = 268/311 (86.17%), Postives = 280/311 (90.03%), Query Frame = 0


Query: 5   KAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCRG 64
           K YSLIIL   F LGA SAEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQCRG
Sbjct: 1   KTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 60

Query: 65  SSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDV 124
           SS+PTPS GSGVGSIISESLYNQMLKYSRDPRCPSNGFY YNAFITAA SFP FGTTGD 
Sbjct: 61  SSTPTPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGDA 120

Query: 125 ATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQKY 184
            TRKRE+AAFFGQTSHETTGG  TAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA GQ+Y
Sbjct: 121 TTRKREIAAFFGQTSHETTGGXSTAPDGPYAWGYCFIRERNQQXYCTPSQQWPCAPGQQY 180

Query: 185 YGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDV 244
           YGRGPIQLTHNYNYGPAG AI + LL+NP+ VAT+ V SFKTA+WFWMT QGNKPSCH+V
Sbjct: 181 YGRGPIQLTHNYNYGPAGKAIGAPLLTNPBTVATDPVTSFKTALWFWMTAQGNKPSCHNV 240

Query: 245 ITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYG 304
           ITG WQPSSAD AAGRVPGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGIGYG
Sbjct: 241 ITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 300

Query: 305 NNLDCYNQRSF 315
           NNLDCYNQRSF
Sbjct: 301 NNLDCYNQRSF 311

[Close]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL
Match: A0A0A0KJK4 (Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507520 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 269/314 (85.67%), Postives = 285/314 (90.76%), Query Frame = 0


Query: 2   KNMKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQ 61
           KNMK YSL+IL   F L  V+AEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQ
Sbjct: 7   KNMKTYSLVILCFAFLLVVVAAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQ 66

Query: 62  CRGSSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 121
           CRGSS+PTPS GSGVGSIISESLYNQMLKYSRD RCPSNGFY YNAFITAA  FP FG T
Sbjct: 67  CRGSSTPTPSGGSGVGSIISESLYNQMLKYSRDSRCPSNGFYTYNAFITAARFFPAFGNT 126

Query: 122 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 181
           G V TRKRE+AAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA+G
Sbjct: 127 GSVETRKREVAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQQAYCTPSQQWPCASG 186

Query: 182 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 241
           Q+YYGRGPIQLTHNYNYGPAGNAI + LL++PDLVAT+AV+SFKTA+WFWMT QGNKPSC
Sbjct: 187 QQYYGRGPIQLTHNYNYGPAGNAIGAPLLASPDLVATDAVVSFKTALWFWMTAQGNKPSC 246

Query: 242 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 301
           H+VITG WQPSSAD AAGR PGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGI
Sbjct: 247 HNVITGNWQPSSADNAAGRSPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGI 306

Query: 302 GYGNNLDCYNQRSF 315
           GYGNNLDCYNQRSF
Sbjct: 307 GYGNNLDCYNQRSF 320

[Close]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL
Match: A0A5A7SHJ5 (Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00730 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 7.8e-164
Identity = 269/312 (86.22%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCR 63
           MK YSL+IL   F LGA SAEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQCR
Sbjct: 1   MKTYSLVILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCR 60

Query: 64  GSSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGD 123
           GSS+P PS GSGVGSIISESLYNQMLKYSRDPRCPSNGFY YNAFITAA SFP FGTTGD
Sbjct: 61  GSSTPPPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGD 120

Query: 124 VATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQK 183
             TRKRE+AAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA GQ+
Sbjct: 121 ATTRKREIAAFFGQTSHETTGGWSTAPDGPYAWGYCFIRERNQQTYCTPSQQWPCAPGQQ 180

Query: 184 YYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHD 243
           YYGRGPIQLTHNYNYGPAG AI + LL+NPD VAT+ V SFKTA+WFWMT QGNKPSCH+
Sbjct: 181 YYGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHN 240

Query: 244 VITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGY 303
           VITG WQPSSAD AAGRVPGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGIGY
Sbjct: 241 VITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGY 300

Query: 304 GNNLDCYNQRSF 315
           GNNLDCYNQRSF
Sbjct: 301 GNNLDCYNQRSF 312

[Close]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.0e-163
Identity = 268/314 (85.35%), Postives = 288/314 (91.72%), Query Frame = 0


Query: 4   MKAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCR 63
           MK ++LI+L   F LG  +AEQCGRQANGALCPNGLCCSQFG+CG+TD+YCK GCQSQC 
Sbjct: 8   MKLHTLIVLAFAFVLGG-AAEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 64  GSSSPTP---SGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTT 123
           GS+  TP    GSGVGSIISESLYNQMLKYS+DPRCPSNGFY YNAF+TAA SFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 124 GDVATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAG 183
           GD ATRKRELAAFFGQTSHETTGGW TAPDGPYAWGYCFIRERNQDVYC+P+QQWPCAAG
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 184 QKYYGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSC 243
           QKYYGRGPIQLTHNYNYGPAGNA+N NLL NPDLVAT+AV+SFKTAIWFWMTPQGNKPSC
Sbjct: 188 QKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 247

Query: 244 HDVITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGI 303
           HDVITGRWQPSSAD+AAGRVPGYGV+TNIINGGLECG GPD RVADRIGF+KRY DMLG+
Sbjct: 248 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 307

Query: 304 GYGNNLDCYNQRSF 315
            YG+NLDC NQRSF
Sbjct: 308 SYGSNLDCNNQRSF 320

[Close]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL
Match: A0A1L3A6A8 (Family 19 chitinase (Fragment) OS=Cucumis melo var. saccharinus OX=1922203 GN=CHT-2 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 269/311 (86.50%), Postives = 280/311 (90.03%), Query Frame = 0


Query: 5   KAYSLIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCRG 64
           K YSLIIL   F LGA SAEQCGRQANGALCPN LCCSQFGFCG+TDDYCK GCQSQCRG
Sbjct: 1   KTYSLIILSFAFLLGAASAEQCGRQANGALCPNNLCCSQFGFCGDTDDYCKNGCQSQCRG 60

Query: 65  SSSPTPS-GSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDV 124
           SS+PTPS GSGVGSIISESLYNQMLKYSRDPRCPSNGFY YNAFITAA SFP FGTTGD 
Sbjct: 61  SSTPTPSGGSGVGSIISESLYNQMLKYSRDPRCPSNGFYTYNAFITAARSFPTFGTTGDA 120

Query: 125 ATRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQKY 184
            TRKRE+AAFFGQTSHETTGG  TAPDGPYAWGYCFIRERNQ  YCTPSQQWPCA GQ+Y
Sbjct: 121 TTRKREIAAFFGQTSHETTGGXSTAPDGPYAWGYCFIRERNQQXYCTPSQQWPCAPGQQY 180

Query: 185 YGRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDV 244
           YGRGPIQLTHNYNYGPAG AI + LL+NPD VAT+ V SFKTA+WFWMT QGNKPSCH+V
Sbjct: 181 YGRGPIQLTHNYNYGPAGKAIGAPLLTNPDTVATDPVTSFKTALWFWMTAQGNKPSCHNV 240

Query: 245 ITGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYG 304
           ITG WQPSSAD AAGRVPGYGV+TNIINGGLECG GPD RV DRIGF+KRYCDMLGIGYG
Sbjct: 241 ITGNWQPSSADNAAGRVPGYGVITNIINGGLECGHGPDDRVKDRIGFYKRYCDMLGIGYG 300

Query: 305 NNLDCYNQRSF 315
           NNLDCYNQRSF
Sbjct: 301 NNLDCYNQRSF 311

[Close]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL
Match: A0A1S3B2Y3 (chitinase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485182 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 4.7e-161
Identity = 265/310 (85.48%), Postives = 282/310 (90.97%), Query Frame = 0


Query: 9   LIILVSVFDLGAVSAEQCGRQANGALCPNGLCCSQFGFCGNTDDYCKTGCQSQCRGSSSP 68
           LIIL   F LG+ SAEQCGRQANGALCPN LCCSQ+G+CG+TD YCK GCQSQCRGS+SP
Sbjct: 8   LIILAFAFLLGSTSAEQCGRQANGALCPNNLCCSQYGWCGDTDAYCKDGCQSQCRGSTSP 67

Query: 69  ----TPSGSGVGSIISESLYNQMLKYSRDPRCPSNGFYAYNAFITAANSFPGFGTTGDVA 128
               TPSGSGVGSII+ESLY QMLKYS D RCPSNGFY YNAFITAA SFPGFGTTGD A
Sbjct: 68  TPPSTPSGSGVGSIITESLYKQMLKYSTDSRCPSNGFYTYNAFITAAQSFPGFGTTGDDA 127

Query: 129 TRKRELAAFFGQTSHETTGGWPTAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAAGQKYY 188
           TRKRELAAFFGQTSHETTGGW +APDGPYAWGYCFIRE NQDVYCTPS QWPC +GQKYY
Sbjct: 128 TRKRELAAFFGQTSHETTGGWASAPDGPYAWGYCFIREINQDVYCTPSSQWPCVSGQKYY 187

Query: 189 GRGPIQLTHNYNYGPAGNAINSNLLSNPDLVATNAVISFKTAIWFWMTPQGNKPSCHDVI 248
           GRGPIQLTHNYNYGPAGNA++ NLLSNPDLVATNA+ISFK AIWFWMTPQGNKPSCH+VI
Sbjct: 188 GRGPIQLTHNYNYGPAGNALSLNLLSNPDLVATNAIISFKAAIWFWMTPQGNKPSCHNVI 247

Query: 249 TGRWQPSSADTAAGRVPGYGVLTNIINGGLECGRGPDGRVADRIGFFKRYCDMLGIGYGN 308
           TG WQPSSAD+AAGRVPGYGVLTNIINGGLECG GPD RV DRIGF+KRYCD+LGIGYG+
Sbjct: 248 TGNWQPSSADSAAGRVPGYGVLTNIINGGLECGHGPDSRVEDRIGFYKRYCDILGIGYGS 307

Query: 309 NLDCYNQRSF 315
           NLDCYNQ+SF
Sbjct: 308 NLDCYNQQSF 317

The following BLAST results are available for this feature:

BLAST of Bhi03G000581 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12500.1	1.9e-130	67.82	basic chitinase	[more]
AT4G01700.1	2.1e-81	56.67	Chitinase family protein	[more]
AT1G02360.1	1.1e-80	53.78	Chitinase family protein	[more]
AT3G54420.1	7.4e-50	36.13	homolog of carrot EP3-3 chitinase	[more]
AT2G43590.1	1.7e-49	36.81	Chitinase family protein	[more]

BLAST of Bhi03G000581 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42993	6.9e-133	67.29	Chitinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht1 PE=2 SV=1	[more]
P93680	3.4e-132	69.71	Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2	[more]
P36907	5.4e-130	67.08	Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P08252	2.1e-129	66.88	Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2	[more]
Q09023	2.1e-129	68.37	Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1	[more]

BLAST of Bhi03G000581 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881302.1	5.3e-191	100.00	endochitinase-like [Benincasa hispida]	[more]
XP_004134833.1	4.2e-164	85.67	endochitinase [Cucumis sativus]	[more]
KAA0025620.1	1.6e-163	86.22	family 19 chitinase [Cucumis melo var. makuwa] >TYK12495.1 family 19 chitinase [...	[more]
XP_022949791.1	6.1e-163	85.35	endochitinase-like [Cucurbita moschata]	[more]
APF47214.1	4.0e-162	86.17	family 19 chitinase, partial [Cucumis melo var. saccharinus]	[more]

BLAST of Bhi03G000581 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJK4	2.1e-164	85.67	Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7SHJ5	7.8e-164	86.22	Family 19 chitinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A6J1GD14	3.0e-163	85.35	endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1	[more]
A0A1L3A6A8	1.1e-162	86.50	Family 19 chitinase (Fragment) OS=Cucumis melo var. saccharinus OX=1922203 GN=CH...	[more]
A0A1S3B2Y3	4.7e-161	85.48	chitinase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485182 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	PRINTS	PR00451	CHITINBINDNG	coord: 41..48 score: 75.0 coord: 48..55 score: 59.83 coord: 33..41 score: 69.78
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 25..62 e-value: 3.0E-16 score: 70.0
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 25..62 e-value: 7.3E-15 score: 55.1
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 23..64 score: 10.836108
None	No IPR available	GENE3D	1.10.530.10		coord: 78..309 e-value: 4.5E-118 score: 394.9
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 161..217 e-value: 4.5E-118 score: 394.9
None	No IPR available	PIRSR	PIRSR001060-2	PIRSR001060-2	coord: 2..314 e-value: 5.6E-142 score: 470.8
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 5..314
None	No IPR available	PANTHER	PTHR22595:SF171	CHITINASE (CLASS IB) / HEVEIN	coord: 5..314
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 79..308 e-value: 4.92433E-111 score: 319.376
None	No IPR available	CDD	cd06921	ChtBD1_GH19_hevein	coord: 24..62 e-value: 2.82779E-18 score: 74.8332
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 23..65 e-value: 4.0E-18 score: 67.2
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 25..68
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 1..314 e-value: 7.0E-124 score: 411.0
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 78..308 e-value: 2.6E-127 score: 423.3
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 221..231
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 35..54
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 75..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000581	Bhi03M000581	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0050832	defense response to fungus
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding