Bhi03G000509 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000509
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	ATP-dependent Clp protease proteolytic subunit
Location	chr3: 12806042 .. 12806251 (+)
RNA-Seq Expression	Bhi03G000509
Synteny	Bhi03G000509

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCCATCAACCTGCTAGTTCTTTTTATGAGGCACAAACGAGAGAATTTATCCTAGCAGCAAAAGAACTACTGAAGCTGCGTGAAACCATCACTAGGGTTTATGTACAAAGAATAGCAAACCCTTATGGGTTGTATCCGAAGACATGGAAAGGGATGTTTTTATGTCAGCAACAGAAGCCCAAGCTCATGGAATTGTTGATCTTGTAG

mRNA sequence

ATGATCCATCAACCTGCTAGTTCTTTTTATGAGGCACAAACGAGAGAATTTATCCTAGCAGCAAAAGAACTACTGAAGCTGCGTGAAACCATCACTAGGGTTTATGTACAAAGAATAGCAAACCCTTATGGGTTGTATCCGAAGACATGGAAAGGGATGTTTTTATGTCAGCAACAGAAGCCCAAGCTCATGGAATTGTTGATCTTGTAG

Coding sequence (CDS)

ATGATCCATCAACCTGCTAGTTCTTTTTATGAGGCACAAACGAGAGAATTTATCCTAGCAGCAAAAGAACTACTGAAGCTGCGTGAAACCATCACTAGGGTTTATGTACAAAGAATAGCAAACCCTTATGGGTTGTATCCGAAGACATGGAAAGGGATGTTTTTATGTCAGCAACAGAAGCCCAAGCTCATGGAATTGTTGATCTTGTAG

Protein sequence

MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQKPKLMELLIL

Homology

[Close]

BLAST of Bhi03G000509 vs. TAIR 10
Match: ATCG00670.1 (plastid-encoded CLP P )

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-13
Identity = 36/42 (85.71%), Postives = 37/42 (88.10%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EFIL A+ELLKLRETITRVYVQR   P
Sbjct: 124 MIHQPASSFYEAQTGEFILEAEELLKLRETITRVYVQRTGKP 165

[Close]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot
Match: A6MM61 (ATP-dependent Clp protease proteolytic subunit OS=Buxus microphylla OX=153571 GN=clpP PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 7.7e-13
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EFIL A+ELLKLRET+TRVYVQR  NP
Sbjct: 124 MIHQPASSFYEAQTGEFILEAEELLKLRETVTRVYVQRTGNP 165

[Close]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot
Match: Q09G21 (ATP-dependent Clp protease proteolytic subunit OS=Platanus occidentalis OX=4403 GN=clpP PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.0e-12
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EFIL A+ELLKLRET+TRVYVQR  NP
Sbjct: 124 MIHQPASSFYEAQTGEFILEAEELLKLRETLTRVYVQRTGNP 165

[Close]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot
Match: Q09FT6 (ATP-dependent Clp protease proteolytic subunit OS=Nandina domestica OX=41776 GN=clpP PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-12
Identity = 35/42 (83.33%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EF+L A+ELLKLRET+TRVYVQR  NP
Sbjct: 124 MIHQPASSFYEAQTGEFVLEAEELLKLRETLTRVYVQRTGNP 165

[Close]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot
Match: A1XGR3 (ATP-dependent Clp protease proteolytic subunit OS=Ranunculus macranthus OX=334596 GN=clpP PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-12
Identity = 35/42 (83.33%), Postives = 38/42 (90.48%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EF+L A+ELLKLRET+TRVYVQR  NP
Sbjct: 123 MIHQPASSFYEAQTGEFVLEAEELLKLRETLTRVYVQRTGNP 164

[Close]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot
Match: A6MMW9 (ATP-dependent Clp protease proteolytic subunit OS=Illicium oligandrum OX=145286 GN=clpP PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-12
Identity = 36/42 (85.71%), Postives = 37/42 (88.10%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP 43
           MIHQPASSFYEAQT EFIL A+ELLKLRETITRVY QR  NP
Sbjct: 124 MIHQPASSFYEAQTGEFILEAEELLKLRETITRVYAQRTGNP 165

[Close]

BLAST of Bhi03G000509 vs. NCBI nr
Match: KRG99245.1 (hypothetical protein GLYMA_18G132600v4 [Glycine max])

HSP 1 Score: 116.7 bits (291), Expect = 7.9e-23
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 1  MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
          MIHQPASSFYEAQT EFIL A+ELLK+RETITRVY +R  N YGLYPKTWKG+FLCQQQK
Sbjct: 1  MIHQPASSFYEAQTGEFILEAEELLKMRETITRVYEKRTENLYGLYPKTWKGIFLCQQQK 60

Query: 61 PKLMEL 67
          PKLMEL
Sbjct: 61 PKLMEL 66

[Close]

BLAST of Bhi03G000509 vs. NCBI nr
Match: KAG6736879.1 (hypothetical protein POTOM_060190 [Populus tomentosa])

HSP 1 Score: 108.2 bits (269), Expect = 2.8e-20
Identity = 53/71 (74.65%), Postives = 58/71 (81.69%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANP--YGLYPKTWKGMFLCQQ 60
           MIHQP ++ YEAQ  EF+L A+ELLKLRE +TRVY QR   P  YGLYPKTWKGMFLCQQ
Sbjct: 67  MIHQPIAAIYEAQIGEFVLEAEELLKLREILTRVYAQRTGKPLCYGLYPKTWKGMFLCQQ 126

Query: 61  QKPKLMELLIL 70
           QKPK MELLIL
Sbjct: 127 QKPKFMELLIL 137

[Close]

BLAST of Bhi03G000509 vs. NCBI nr
Match: VDD24611.1 (unnamed protein product, partial [Brassica rapa])

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 55/72 (76.39%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQ-RIANPYGL--YPKTWKGMFLCQ 60
           MIHQPASSFYEAQT E IL A+ELLKLRETITRVY + +    YGL    KTWKGMFLCQ
Sbjct: 50  MIHQPASSFYEAQTGEVILEAEELLKLRETITRVYTKNKEQTKYGLGCIMKTWKGMFLCQ 109

Query: 61  QQKPKLMELLIL 70
           QQKPKLM LLIL
Sbjct: 110 QQKPKLMALLIL 121

[Close]

BLAST of Bhi03G000509 vs. NCBI nr
Match: TYG40841.1 (hypothetical protein ES288_D12G126000v1 [Gossypium darwinii])

HSP 1 Score: 88.6 bits (218), Expect = 2.3e-14
Identity = 48/68 (70.59%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 1  MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
          MIHQPASSFYEAQT EFIL A+ELLKLRE++TRVY             TWK MFLCQQQK
Sbjct: 11 MIHQPASSFYEAQTGEFILEAEELLKLRESLTRVY-------------TWKEMFLCQQQK 65

Query: 61 PKLMELLI 69
          PKLMEL I
Sbjct: 71 PKLMELWI 65

[Close]

BLAST of Bhi03G000509 vs. NCBI nr
Match: VDD65251.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 88.2 bits (217), Expect = 3.0e-14
Identity = 48/69 (69.57%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
           MIHQPASSFYEAQT E IL A+ELLKLRETITRVYVQR   P  +  +  +   LCQQQK
Sbjct: 77  MIHQPASSFYEAQTGEVILEAEELLKLRETITRVYVQRTGKPIWVVSEDMERDVLCQQQK 136

Query: 61  PKLMELLIL 70
            KLM LLIL
Sbjct: 137 AKLMALLIL 145

[Close]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL
Match: K7MRU4 (Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_18G132600 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.8e-23
Identity = 56/66 (84.85%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 1  MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
          MIHQPASSFYEAQT EFIL A+ELLK+RETITRVY +R  N YGLYPKTWKG+FLCQQQK
Sbjct: 1  MIHQPASSFYEAQTGEFILEAEELLKMRETITRVYEKRTENLYGLYPKTWKGIFLCQQQK 60

Query: 61 PKLMEL 67
          PKLMEL
Sbjct: 61 PKLMEL 66

[Close]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL
Match: A0A3P6DM77 (Uncharacterized protein (Fragment) OS=Brassica campestris OX=3711 GN=BRASC58T46495Z PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.3e-18
Identity = 55/72 (76.39%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQ-RIANPYGL--YPKTWKGMFLCQ 60
           MIHQPASSFYEAQT E IL A+ELLKLRETITRVY + +    YGL    KTWKGMFLCQ
Sbjct: 50  MIHQPASSFYEAQTGEVILEAEELLKLRETITRVYTKNKEQTKYGLGCIMKTWKGMFLCQ 109

Query: 61  QQKPKLMELLIL 70
           QQKPKLM LLIL
Sbjct: 110 QQKPKLMALLIL 121

[Close]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL
Match: A0A6N2N221 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS430238 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-15
Identity = 44/57 (77.19%), Postives = 47/57 (82.46%), Query Frame = 0


Query: 13  QTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQKPKLMELLIL 70
           Q  EF+L A+ELLKLRE +TRVY QR   PYGLY KTWKGMFLCQQQKPK MELLIL
Sbjct: 44  QIGEFVLEAEELLKLREILTRVYAQRTGKPYGLYLKTWKGMFLCQQQKPKFMELLIL 100

[Close]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL
Match: A0A5D2A7L5 (Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_D12G126000v1 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-14
Identity = 48/68 (70.59%), Postives = 51/68 (75.00%), Query Frame = 0


Query: 1  MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
          MIHQPASSFYEAQT EFIL A+ELLKLRE++TRVY             TWK MFLCQQQK
Sbjct: 11 MIHQPASSFYEAQTGEFILEAEELLKLRESLTRVY-------------TWKEMFLCQQQK 65

Query: 61 PKLMELLI 69
          PKLMEL I
Sbjct: 71 PKLMELWI 65

[Close]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL
Match: A0A3P6H1B9 (ATP-dependent Clp protease proteolytic subunit OS=Brassica oleracea OX=3712 GN=BOLSC29T60479H PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-14
Identity = 48/69 (69.57%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 1   MIHQPASSFYEAQTREFILAAKELLKLRETITRVYVQRIANPYGLYPKTWKGMFLCQQQK 60
           MIHQPASSFYEAQT E IL A+ELLKLRETITRVYVQR   P  +  +  +   LCQQQK
Sbjct: 77  MIHQPASSFYEAQTGEVILEAEELLKLRETITRVYVQRTGKPIWVVSEDMERDVLCQQQK 136

Query: 61  PKLMELLIL 70
            KLM LLIL
Sbjct: 137 AKLMALLIL 145

The following BLAST results are available for this feature:

BLAST of Bhi03G000509 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00670.1	2.1e-13	85.71	plastid-encoded CLP P	[more]

BLAST of Bhi03G000509 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A6MM61	7.7e-13	85.71	ATP-dependent Clp protease proteolytic subunit OS=Buxus microphylla OX=153571 GN...	[more]
Q09G21	1.0e-12	85.71	ATP-dependent Clp protease proteolytic subunit OS=Platanus occidentalis OX=4403 ...	[more]
Q09FT6	1.3e-12	83.33	ATP-dependent Clp protease proteolytic subunit OS=Nandina domestica OX=41776 GN=...	[more]
A1XGR3	1.3e-12	83.33	ATP-dependent Clp protease proteolytic subunit OS=Ranunculus macranthus OX=33459...	[more]
A6MMW9	1.7e-12	85.71	ATP-dependent Clp protease proteolytic subunit OS=Illicium oligandrum OX=145286 ...	[more]

BLAST of Bhi03G000509 vs. NCBI nr


Match Name	E-value	Identity	Description
KRG99245.1	7.9e-23	84.85	hypothetical protein GLYMA_18G132600v4 [Glycine max]	[more]
KAG6736879.1	2.8e-20	74.65	hypothetical protein POTOM_060190 [Populus tomentosa]	[more]
VDD24611.1	1.7e-17	76.39	unnamed protein product, partial [Brassica rapa]	[more]
TYG40841.1	2.3e-14	70.59	hypothetical protein ES288_D12G126000v1 [Gossypium darwinii]	[more]
VDD65251.1	3.0e-14	69.57	unnamed protein product [Brassica oleracea]	[more]

BLAST of Bhi03G000509 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7MRU4	3.8e-23	84.85	Uncharacterized protein OS=Glycine max OX=3847 GN=GLYMA_18G132600 PE=4 SV=1	[more]
A0A3P6DM77	8.3e-18	76.39	Uncharacterized protein (Fragment) OS=Brassica campestris OX=3711 GN=BRASC58T464...	[more]
A0A6N2N221	1.3e-15	77.19	Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS430238 PE=4 SV=...	[more]
A0A5D2A7L5	1.1e-14	70.59	Uncharacterized protein OS=Gossypium darwinii OX=34276 GN=ES288_D12G126000v1 PE=...	[more]
A0A3P6H1B9	1.5e-14	69.57	ATP-dependent Clp protease proteolytic subunit OS=Brassica oleracea OX=3712 GN=B...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 1..65 e-value: 1.8E-5 score: 26.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000509	Bhi03M000509	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0009368	endopeptidase Clp complex
cellular_component	GO:0016020	membrane
cellular_component	GO:0009526	plastid envelope
molecular_function	GO:0051117	ATPase binding
molecular_function	GO:0004176	ATP-dependent peptidase activity
molecular_function	GO:0004252	serine-type endopeptidase activity