Bhi03G000463 (gene) Wax gourd (B227) v1

BLAST of Bhi03G000463 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 677.9 bits (1748), Expect = 6.2e-195
Identity = 332/509 (65.23%), Postives = 395/509 (77.60%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           L+VS LL        A +  N+G  R+GLKK+K D   R  A +ESK+ + L +      
Sbjct: 12  LIVSFLLCF-----SAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------ 71

Query: 69  QWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSL 128
                +G+S + D+V LKNYLDAQYYGEI IGTPPQKFTVVFDTGSSNLWVPSSKC FSL
Sbjct: 72  ---YRLGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSL 131

Query: 129 ACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMS 188
           AC  H +Y+S RSSTY +NG +A+I YG+GAIAGFFS D V VGD+VV DQE IEAT   
Sbjct: 132 ACLLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEP 191

Query: 189 SMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELV 248
            +TF+ AKFDGILGLGFQEIS G A PVWYNM+KQ L+KE VFSFWLNRNA E EGGELV
Sbjct: 192 GITFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELV 251

Query: 249 FGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPS 308
           FGGVDP HFKG+HTYVPVT KGYWQFD+GD+LIGG  T +C  GCSAIADSGTSLLAGP+
Sbjct: 252 FGGVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPT 311

Query: 309 TIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVR 368
           TI+ +IN AIGAA V S +CK +V Q+GQ I+DLLL   QP+KICS+IG+CTFD TRGV 
Sbjct: 312 TIITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVS 371

Query: 369 LKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDR-GLN 428
           + IE++V+ ++ K S G  DA CSACEMAV W+Q +L+QN TQE I++YVNELC+R    
Sbjct: 372 MGIESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSP 431

Query: 429 QGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGP 488
            G + VDC ++S MPTVS TIG +VF+L+ ++Y+LKVGEG  AQCISGFI LD+ PPRGP
Sbjct: 432 MGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGP 491

Query: 489 LWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           LWILGDVFMG+YHTVFDFG  +VGFA+AA
Sbjct: 492 LWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of Bhi03G000463 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 675.2 bits (1741), Expect = 4.0e-194
Identity = 320/509 (62.87%), Postives = 401/509 (78.78%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           + VS LL    YS       N+G  R+GLKK+K D N R      SK+ E L SS+  +N
Sbjct: 12  VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYN 71

Query: 69  QWGNNI-GESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFS 128
              NN+ G+S + DIV LKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWVPS KC FS
Sbjct: 72  ---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFS 131

Query: 129 LACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSM 188
           L+C+FHA+Y+S RSSTY+++G  A+I YGSG+I+GFFSYD V VGD+VV DQE IE TS 
Sbjct: 132 LSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSE 191

Query: 189 SSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGEL 248
             +TF+ AKFDG+LGLGFQEI+ G A PVWYNM+KQ L+K  VFSFWLNR+    EGGE+
Sbjct: 192 PGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEI 251

Query: 249 VFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGP 308
           VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSGTSLLAGP
Sbjct: 252 VFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGP 311

Query: 309 STIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGV 368
           + +VA+IN+AIGA+ V S +CK +V Q+GQ I+DLLL   QP+KICS+IG+C +D T GV
Sbjct: 312 TAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGV 371

Query: 369 RLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLN 428
            + IE++V+ ++ +SS G  DA C ACEMAV W+Q +L+QN TQE I++Y+NE+C+R  +
Sbjct: 372 SMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPS 431

Query: 429 -QGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRG 488
             G + VDC ++S+MPTVSFTIG +VF+L+ ++Y+LK+GEG  AQCISGF  LDIPPPRG
Sbjct: 432 PNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRG 491

Query: 489 PLWILGDVFMGRYHTVFDFGKVRVGFADA 516
           PLWILGDVFMG+YHTVFDFG  +VGFA+A
Sbjct: 492 PLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Bhi03G000463 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 675.2 bits (1741), Expect = 4.0e-194
Identity = 320/509 (62.87%), Postives = 401/509 (78.78%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           + VS LL    YS       N+G  R+GLKK+K D N R      SK+ E L SS+  +N
Sbjct: 12  VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYN 71

Query: 69  QWGNNI-GESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFS 128
              NN+ G+S + DIV LKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWVPS KC FS
Sbjct: 72  ---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFS 131

Query: 129 LACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSM 188
           L+C+FHA+Y+S RSSTY+++G  A+I YGSG+I+GFFSYD V VGD+VV DQE IE TS 
Sbjct: 132 LSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSE 191

Query: 189 SSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGEL 248
             +TF+ AKFDG+LGLGFQEI+ G A PVWYNM+KQ L+K  VFSFWLNR+    EGGE+
Sbjct: 192 PGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEI 251

Query: 249 VFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGP 308
           VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSGTSLLAGP
Sbjct: 252 VFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGP 311

Query: 309 STIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGV 368
           + +VA+IN+AIGA+ V S +CK +V Q+GQ I+DLLL   QP+KICS+IG+C +D T GV
Sbjct: 312 TAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGV 371

Query: 369 RLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLN 428
            + IE++V+ ++ +SS G  DA C ACEMAV W+Q +L+QN TQE I++Y+NE+C+R  +
Sbjct: 372 SMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPS 431

Query: 429 -QGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRG 488
             G + VDC ++S+MPTVSFTIG +VF+L+ ++Y+LK+GEG  AQCISGF  LDIPPPRG
Sbjct: 432 PNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRG 491

Query: 489 PLWILGDVFMGRYHTVFDFGKVRVGFADA 516
           PLWILGDVFMG+YHTVFDFG  +VGFA+A
Sbjct: 492 PLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Bhi03G000463 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 622.5 bits (1604), Expect = 3.1e-178
Identity = 301/509 (59.14%), Postives = 386/509 (75.83%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           LLV LL  ++  S+ +   + +G +RIGLKK K D++ R  + L  K     GS     +
Sbjct: 9   LLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKN---RGSHWSPKH 68

Query: 69  QWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSL 128
            +  N     N D+V LKNYLDAQYYG+I IGTPPQKFTV+FDTGSSNLW+PS+KC  S+
Sbjct: 69  YFRLN---DENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYLSV 128

Query: 129 ACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMS 188
           AC+FH++Y++ +SS+YR+NG  ASI+YG+GAI+G+FS D+V+VGD+VV +QE IEATS  
Sbjct: 129 ACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATSEP 188

Query: 189 SMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELV 248
            +TF+ AKFDGILGLGF+EIS G + PVWYNMV++ LVKE +FSFWLNRN  + EGGE+V
Sbjct: 189 GITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGEIV 248

Query: 249 FGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPS 308
           FGGVDPKHFKG+HT+VPVT KGYWQFD+GD+ I G+ T YCA GCSAIADSGTSLL GPS
Sbjct: 249 FGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTGPS 308

Query: 309 TIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVR 368
           T++ +IN AIGA  + S ECKA+V Q+G+ +++ LL    P+K+CS+IGVC +D T+ V 
Sbjct: 309 TVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGVCAYDGTQSVS 368

Query: 369 LKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLNQ 428
           + I+++V+D    +SG  + AMCSACEMA  WM+ EL QN+TQE I+ Y  ELCD    Q
Sbjct: 369 MGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQ 428

Query: 429 G-ATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGP 488
              + VDCGR+S MP V+F+IG R F+L+ +DYI K+GEG  +QC SGF  +DI PPRGP
Sbjct: 429 NQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGP 488

Query: 489 LWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           LWILGD+FMG YHTVFD+GK RVGFA AA
Sbjct: 489 LWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

BLAST of Bhi03G000463 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 614.4 bits (1583), Expect = 8.5e-176
Identity = 300/509 (58.94%), Postives = 385/509 (75.64%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           LLV LL  ++  S+ +   + +G +RIGLKK K D++ R  + L  K     GS     +
Sbjct: 9   LLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKN---RGSHWSPKH 68

Query: 69  QWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSL 128
            +  N     N D+V LKNYLDAQYYG+I IGTPPQKFTV+FDTGSSNLW+PS+KC  S+
Sbjct: 69  YFRLN---DENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYLSV 128

Query: 129 ACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMS 188
           AC+FH++Y++ +SS+YR+NG  ASI+YG+GAI+G+FS D+V+VGD+VV +QE IEATS  
Sbjct: 129 ACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATSEP 188

Query: 189 SMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELV 248
            +TF+ AKFDGILGLGF+EIS G + PVWYNMV++ LVKE +FSFWLNRN  + EGGE+V
Sbjct: 189 GITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGEIV 248

Query: 249 FGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPS 308
           FGGVDPKHFKG+HT+VPVT KGYWQFD+GD+ I G+ T YCA GCSAIADSGTSLL GPS
Sbjct: 249 FGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTGPS 308

Query: 309 TIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVR 368
           T++ +IN AIGA  + S ECKA+V Q+G+ +++ LL     +K+CS+IGVC +D T+ V 
Sbjct: 309 TVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLA----QKVCSQIGVCAYDGTQSVS 368

Query: 369 LKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLNQ 428
           + I+++V+D    +SG  + AMCSACEMA  WM+ EL QN+TQE I+ Y  ELCD    Q
Sbjct: 369 MGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQ 428

Query: 429 G-ATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGP 488
              + VDCGR+S MP V+F+IG R F+L+ +DYI K+GEG  +QC SGF  +DI PPRGP
Sbjct: 429 NQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGP 488

Query: 489 LWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           LWILGD+FMG YHTVFD+GK RVGFA AA
Sbjct: 489 LWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

BLAST of Bhi03G000463 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 6.1e-203
Identity = 341/505 (67.52%), Postives = 413/505 (81.78%), Query Frame = 0

Query: 13  LLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHNQWGN 72
           L L +L   +  SSASN+G LR+GLKKIK D   R  A +ESK  E L ++  K+N  G 
Sbjct: 11  LCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKG- 70

Query: 73  NIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSLACFF 132
           N+GES +TDIVALKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWV   +C+FS+AC F
Sbjct: 71  NLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCECLFSVACHF 130

Query: 133 HARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMSSMTF 192
           HARY+S RSS+Y++NGTSASI+YG+GA++GFFSYDNV+VGD+VV +Q  IEAT   S+TF
Sbjct: 131 HARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTF 190

Query: 193 MTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELVFGGV 252
           + AKFDG+LGLGFQEI+ G AVPVWYNMV+Q LVKE VFSFWLNRN  E EGGE+VFGGV
Sbjct: 191 LVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGV 250

Query: 253 DPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPSTIVA 312
           DPKH++G+HTYVPVT KGYWQFD+GD+LI GE T +C  GCSAIADSGTSLLAGP+ ++ 
Sbjct: 251 DPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVIT 310

Query: 313 LINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVRLKIE 372
           +IN AIGA  V S +CKA+V+Q+GQ IMDLLL  A P+KICS+I +CTFD TRGV + IE
Sbjct: 311 MINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIE 370

Query: 373 TLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLN-QGAT 432
           ++V++  GKSS    D MCS CEM V WMQ++L+QN+T+E II+Y+NELCDR  +  G +
Sbjct: 371 SVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQS 430

Query: 433 LVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGPLWIL 492
            VDCG++S MPTVSFTIG ++F+L+ ++YILKVGEG  AQCISGF   DIPPPRGPLWIL
Sbjct: 431 AVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWIL 490

Query: 493 GDVFMGRYHTVFDFGKVRVGFADAA 517
           GDVFMGRYHTVFDFGK+RVG A+AA
Sbjct: 491 GDVFMGRYHTVFDFGKLRVGSAEAA 513

BLAST of Bhi03G000463 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 8.8e-194
Identity = 332/509 (65.23%), Postives = 395/509 (77.60%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           L+VS LL        A +  N+G  R+GLKK+K D   R  A +ESK+ + L +      
Sbjct: 12  LIVSFLLCF-----SAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------ 71

Query: 69  QWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSL 128
                +G+S + D+V LKNYLDAQYYGEI IGTPPQKFTVVFDTGSSNLWVPSSKC FSL
Sbjct: 72  ---YRLGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSL 131

Query: 129 ACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMS 188
           AC  H +Y+S RSSTY +NG +A+I YG+GAIAGFFS D V VGD+VV DQE IEAT   
Sbjct: 132 ACLLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEP 191

Query: 189 SMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELV 248
            +TF+ AKFDGILGLGFQEIS G A PVWYNM+KQ L+KE VFSFWLNRNA E EGGELV
Sbjct: 192 GITFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELV 251

Query: 249 FGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPS 308
           FGGVDP HFKG+HTYVPVT KGYWQFD+GD+LIGG  T +C  GCSAIADSGTSLLAGP+
Sbjct: 252 FGGVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPT 311

Query: 309 TIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVR 368
           TI+ +IN AIGAA V S +CK +V Q+GQ I+DLLL   QP+KICS+IG+CTFD TRGV 
Sbjct: 312 TIITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVS 371

Query: 369 LKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDR-GLN 428
           + IE++V+ ++ K S G  DA CSACEMAV W+Q +L+QN TQE I++YVNELC+R    
Sbjct: 372 MGIESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSP 431

Query: 429 QGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGP 488
            G + VDC ++S MPTVS TIG +VF+L+ ++Y+LKVGEG  AQCISGFI LD+ PPRGP
Sbjct: 432 MGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGP 491

Query: 489 LWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           LWILGDVFMG+YHTVFDFG  +VGFA+AA
Sbjct: 492 LWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of Bhi03G000463 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 5.7e-193
Identity = 320/509 (62.87%), Postives = 401/509 (78.78%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHN 68
           + VS LL    YS       N+G  R+GLKK+K D N R      SK+ E L SS+  +N
Sbjct: 12  VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYN 71

Query: 69  QWGNNI-GESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFS 128
              NN+ G+S + DIV LKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWVPS KC FS
Sbjct: 72  ---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFS 131

Query: 129 LACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSM 188
           L+C+FHA+Y+S RSSTY+++G  A+I YGSG+I+GFFSYD V VGD+VV DQE IE TS 
Sbjct: 132 LSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSE 191

Query: 189 SSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGEL 248
             +TF+ AKFDG+LGLGFQEI+ G A PVWYNM+KQ L+K  VFSFWLNR+    EGGE+
Sbjct: 192 PGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEI 251

Query: 249 VFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGP 308
           VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSGTSLLAGP
Sbjct: 252 VFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGP 311

Query: 309 STIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGV 368
           + +VA+IN+AIGA+ V S +CK +V Q+GQ I+DLLL   QP+KICS+IG+C +D T GV
Sbjct: 312 TAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGV 371

Query: 369 RLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDRGLN 428
            + IE++V+ ++ +SS G  DA C ACEMAV W+Q +L+QN TQE I++Y+NE+C+R  +
Sbjct: 372 SMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPS 431

Query: 429 -QGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRG 488
             G + VDC ++S+MPTVSFTIG +VF+L+ ++Y+LK+GEG  AQCISGF  LDIPPPRG
Sbjct: 432 PNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRG 491

Query: 489 PLWILGDVFMGRYHTVFDFGKVRVGFADA 516
           PLWILGDVFMG+YHTVFDFG  +VGFA+A
Sbjct: 492 PLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of Bhi03G000463 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 655.6 bits (1690), Expect = 4.7e-187
Identity = 322/508 (63.39%), Postives = 395/508 (77.76%), Query Frame = 0

Query: 10  LVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKHNQ 69
           LV L  ++L     AS+A  EG +RI LKK   D+N R  A L  ++G       G  N 
Sbjct: 8   LVLLAAVLLQALLPASAA--EGLVRIALKKRPIDENSRVAARLSGEEGARRLGLRGA-NS 67

Query: 70  WGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFSLA 129
            G   GE    DIVALKNY++AQY+GEIG+GTPPQKFTV+FDTGSSNLWVPS+KC FS+A
Sbjct: 68  LGGGGGEG---DIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIA 127

Query: 130 CFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSMSS 189
           CFFH+RY+SG+SSTY++NG  A+IQYG+G+IAGFFS D+V VGD+VV DQE IEAT    
Sbjct: 128 CFFHSRYKSGQSSTYQKNGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKEPG 187

Query: 190 MTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGELVF 249
           +TFM AKFDGILGLGFQEIS G AVPVWY MV+Q LV E VFSFW NR++ E EGGE+VF
Sbjct: 188 LTFMVAKFDGILGLGFQEISVGDAVPVWYKMVEQGLVSEPVFSFWFNRHSDEGEGGEIVF 247

Query: 250 GGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGPST 309
           GG+DP H+KG HTYVPV+ KGYWQF++GD+LIGG+TT +CA GCSAIADSGTSLLAGP+ 
Sbjct: 248 GGMDPSHYKGNHTYVPVSQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLAGPTA 307

Query: 310 IVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGVRL 369
           I+  IN  IGA  V S ECK +VSQ+GQ I+DLLL   QP KICS++G+CTFD   GV  
Sbjct: 308 IITEINEKIGATGVVSQECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSA 367

Query: 370 KIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDR-GLNQ 429
            I+++V+D+ G+S+G  S  MC+ACEMAV WMQ++L QNKTQ+ I++Y+N+LCD+     
Sbjct: 368 GIKSVVDDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPM 427

Query: 430 GATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRGPL 489
           G + VDCG ++ MP +SFTIG + F L  ++YILKVGEG+AAQCISGF  +DIPPPRGPL
Sbjct: 428 GESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPL 487

Query: 490 WILGDVFMGRYHTVFDFGKVRVGFADAA 517
           WILGDVFMG YHTVFD+GK+RVGFA +A
Sbjct: 488 WILGDVFMGAYHTVFDYGKMRVGFAKSA 509

BLAST of Bhi03G000463 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.7e-185
Identity = 314/510 (61.57%), Postives = 393/510 (77.06%), Query Frame = 0

Query: 9   LLVSLLLLILYYSSEASSASN-EGFLRIGLKKIKADQNGRFKALLESKKGEFLGSSVGKH 68
           L ++LL  +L   +   +AS  EG +RI LKK   D+N R    L   + + L S     
Sbjct: 6   LALALLAAVLLLQTVLPAASEAEGLVRIALKKRPIDRNSRVATGLSGGEEQPLLSG---- 65

Query: 69  NQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSSKCIFS 128
               N +      DIVALKNY++AQY+GEIG+GTPPQKFTV+FDTGSSNLWVPS+KC FS
Sbjct: 66  ---ANPLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFS 125

Query: 129 LACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQELIEATSM 188
           +AC+ H+RY++G SSTY++NG  A+IQYG+G+IAG+FS D+V VGD+VV DQE IEAT  
Sbjct: 126 IACYLHSRYKAGASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEATKE 185

Query: 189 SSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGEAEGGEL 248
             +TF+ AKFDGILGLGF+EIS G AVPVWY M++Q LV + VFSFWLNR+  E EGGE+
Sbjct: 186 PGITFLVAKFDGILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHVDEGEGGEI 245

Query: 249 VFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGTSLLAGP 308
           +FGG+DPKH+ G+HTYVPVT KGYWQFD+GD+L+GG++T +CA GC+AIADSGTSLLAGP
Sbjct: 246 IFGGMDPKHYVGEHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSLLAGP 305

Query: 309 STIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTFDDTRGV 368
           + I+  IN  IGAA V S ECK IVSQ+GQ I+DLLL   QP+KICS++G+CTFD TRGV
Sbjct: 306 TAIITEINEKIGAAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDGTRGV 365

Query: 369 RLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNELCDR-GL 428
              I ++V+D+  KS+G  +D MCSACEMAV WMQ++L QNKTQ+ I+DYVN+LC+R   
Sbjct: 366 SAGIRSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPS 425

Query: 429 NQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPPRG 488
             G + VDCG +  MP + FTIG + F L  ++YILKVGEG+AAQCISGF  +DIPPPRG
Sbjct: 426 PMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRG 485

Query: 489 PLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           PLWILGDVFMG YHTVFD+GK+R+GFA AA
Sbjct: 486 PLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

BLAST of Bhi03G000463 vs. NCBI nr
Match: XP_038880988.1 (aspartic proteinase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1044.6 bits (2700), Expect = 2.7e-301
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL
Sbjct: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP
Sbjct: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE
Sbjct: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG
Sbjct: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG
Sbjct: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT
Sbjct: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE
Sbjct: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA
Sbjct: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 516

BLAST of Bhi03G000463 vs. NCBI nr
Match: XP_038880987.1 (aspartic proteinase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1044.6 bits (2700), Expect = 2.7e-301
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL
Sbjct: 21  MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 80

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP
Sbjct: 81  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 140

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE
Sbjct: 141 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 200

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG
Sbjct: 201 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 260

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG
Sbjct: 261 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 320

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT
Sbjct: 321 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 380

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE
Sbjct: 381 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 440

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 441 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 500

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA
Sbjct: 501 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 536

BLAST of Bhi03G000463 vs. NCBI nr
Match: XP_008440021.1 (PREDICTED: aspartic proteinase-like isoform X1 [Cucumis melo])

HSP 1 Score: 930.6 bits (2404), Expect = 5.7e-267
Identity = 458/516 (88.76%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 33  MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 92

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           G SVGKHNQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 93  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 152

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 153 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 212

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 213 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 272

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 273 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 332

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 333 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 392

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 393 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 452

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 453 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 512

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 513 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 548

BLAST of Bhi03G000463 vs. NCBI nr
Match: XP_008440022.1 (PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440023.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440024.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440026.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_016899345.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo])

HSP 1 Score: 930.6 bits (2404), Expect = 5.7e-267
Identity = 458/516 (88.76%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           G SVGKHNQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of Bhi03G000463 vs. NCBI nr
Match: TYK13029.1 (aspartic proteinase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 930.2 bits (2403), Expect = 7.5e-267
Identity = 458/516 (88.76%), Postives = 483/516 (93.60%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           GSSVGK+NQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GSSVGKYNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of Bhi03G000463 vs. ExPASy TrEMBL
Match: A0A1S3B040 (aspartic proteinase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.8e-267
Identity = 458/516 (88.76%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           G SVGKHNQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of Bhi03G000463 vs. ExPASy TrEMBL
Match: A0A1S3B058 (aspartic proteinase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.8e-267
Identity = 458/516 (88.76%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 33  MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 92

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           G SVGKHNQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 93  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 152

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 153 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 212

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 213 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 272

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 273 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 332

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 333 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 392

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 393 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 452

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 453 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 512

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 513 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 548

BLAST of Bhi03G000463 vs. ExPASy TrEMBL
Match: A0A5D3CRY9 (Aspartic proteinase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006300 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.6e-267
Identity = 458/516 (88.76%), Postives = 483/516 (93.60%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKI++DQN RFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           GSSVGK+NQWGNN+GES+N D V LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GSSVGKYNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           SSKC+FSLACFFHARYQSGRSSTY++NGTSA+IQYGSGAIAGFFS DNVRVGDVVV +Q+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMSSMTFM AKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV LINRAIGAA VA PECKAIVSQHG+AIMDLLL  AQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD TR V LKIE +V+DKDG+SSGGFS+AMCSACEMAVSW+QDEL+QNKTQE IID VNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRG NQ  TLVDCGRIS+MP+VSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFADAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of Bhi03G000463 vs. ExPASy TrEMBL
Match: A0A0A0KMZ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G513550 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.3e-264
Identity = 451/516 (87.40%), Postives = 481/516 (93.22%), Query Frame = 0

Query: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKIK DQN RFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120
           GSSVGKHNQWGNN+ ES+N DIV LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180
           S+KCIFSLACFFHA+YQSGRSSTY+RNGTSA+IQYGSGAI+GFFSYDNV+VGDV+V +QE
Sbjct: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180

Query: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240
           LIEATSMS+MTFM AKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240

Query: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300

Query: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360
           TSLLAGPS IV  INRAIGAA VA PECKAIVSQ+G+AIMDLLL  AQPEKICSKIGVCT
Sbjct: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360

Query: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420
           FD+T  V LKIE +V+DKDG+SSGGFS+AMCSACEMAV W+QDELKQNKTQE II+ VNE
Sbjct: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420

Query: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480
           LCDRGLNQ  TLVDCGRIS+MP VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480

Query: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516

BLAST of Bhi03G000463 vs. ExPASy TrEMBL
Match: A0A6J1IKS1 (aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477945 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.8e-257
Identity = 436/515 (84.66%), Postives = 476/515 (92.43%), Query Frame = 0

Query: 2   MRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFLG 61
           MRNS KPLLVSLLLLIL YSS ASSASNEG +RIGLKKIK ++N   KAL+ESKK EFLG
Sbjct: 1   MRNSLKPLLVSLLLLIL-YSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVEFLG 60

Query: 62  SSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPS 121
           S   KH+QWGN++GES+N+DIVALKNY+DAQYYGEIGIGTPPQKFTV+FDTGSSNLWVPS
Sbjct: 61  S---KHDQWGNDLGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPS 120

Query: 122 SKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQEL 181
           SKC+FS+ACFFHARYQSGRSSTYRRNGTSA+IQYGSGAI+GFFSYDNV+VGDVVV +Q+ 
Sbjct: 121 SKCVFSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRNQQF 180

Query: 182 IEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAGE 241
           IE TSMSSMTF+ AKFDGILGLGFQEISTG AVPVWYNMVKQKLVKE VFSFWLNRNA E
Sbjct: 181 IETTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVKQKLVKEPVFSFWLNRNAEE 240

Query: 242 AEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSGT 301
            EGGE+VFGGVDPKHFKGQHTYVPVTTKGYWQF+IGDILIGG+ TEYCA GCSAIADSGT
Sbjct: 241 EEGGEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCARGCSAIADSGT 300

Query: 302 SLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCTF 361
           SLLAGPSTIV LINRAIGAA +  PECK +VSQHG++IMDLLL   QPEKICSKIGVC F
Sbjct: 301 SLLAGPSTIVTLINRAIGAAGIGRPECKEVVSQHGKSIMDLLLAKVQPEKICSKIGVCAF 360

Query: 362 DDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNEL 421
           D + GV  KIE++VN+KDG SSGGFSDAMCSACEMAVSWM DELKQNKTQEH+IDYVN+L
Sbjct: 361 DGSHGVSKKIESVVNEKDGTSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKL 420

Query: 422 CDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDI 481
           CDR LN+G TLVDCGRIS+MPTVSFTIGD+VFEL+++DYILKVGEGSAAQCISGFIPLDI
Sbjct: 421 CDRDLNEGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDI 480

Query: 482 PPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 517
           PPPRGPLWILGDVFMGRYHTVFDFGK+RVGFA+AA
Sbjct: 481 PPPRGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 511