Bhi03G000323 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000323
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	chr3: 8331068 .. 8334249 (+)
RNA-Seq Expression	Bhi03G000323
Synteny	Bhi03G000323

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCACTAGGATGTTAAAAGAAAACGGAGGTTCGTCAATAAAACCCCAATCTCACGGGACGTTTTACGCTCCATCATTATCAACTCCTTCTTATCTCTGTTTCCCGTTCTGTCTTTTCGTTCTTCAACCTTTTCATTTTTGCATTAACATTGCCTCTTTTCTGTGGAACCAATCTCTTTCTCTCAGGTAAACCACTGAATTTGAACTTTCATGAATAGTTCTCGATGTTTTTCTCTTCCCAGCTTCTGTATTCTTGCTCCGTTTCGCTGTGATTGTGTCTGTTGAGGTGAAAGTTTTGATGGCTTCTTTCTATTTGTTGATTAATTCCTTTTCTTTTCAACTTCTTTGTCTTTGTTTGTGATGTTTCATTCCAGCGATCGCGCGCTCGGATTTTCTGATGGCATTTCTCGTTTCTTGGATCTTTCAGTCTGGATGAGTTTTTCTGTTTGTGGCTCTTTTTCTTTCCACCGTGTGTTTGGTTTTAATTTTGAGAATTGGGAAAGTTGTATTTGGCTGTTCGTAGGAGAATTCTATGTATATTAGAGCACACTACTGGTTTCACTAGAAATTCTTTAGAATGTACGGCCTACTGACTGTACCATATATATCTATGATTAGAGTTTTCTCATTCTATGCTTGGAAGAGCAGGGTTTCTTATGGATCCTTGCGGCTTTGGTTGATGAATTTTCATCACTGAGAGCCTTGTCAGGCCATCGACCACTTTTATTTGTTTCTGGCCGATGGAAAGTAATCCGAGCAACACACACTAACACAGTGGAGTTATTTAGCCTTTGCCTCTGACGGTTTGGTTTCGTTTGCTAACAAGATCTGTTCTTATATGTCCATCAAAAAACTACAAATTAGGACAGCAGAGATTTCTTTCACTGAGGAGAAATTTTTTCTCTCTGAATTCTGTATATGTATATTTTTCGTGTGAATGATTTTGAACTAGCAGTGTACGGGAGATATCCTCTTGTTTCCTTTCTGCTGTGTGCAGCATTTATTACATTATCTGTCACGAGTTCAGGAAATTTCGATCCATTTGATTTTGGGCGCTTTAAGGAAAACTAGAAGAGGATTTAGTTTCCTAGAGTTACCAATTGAAGTTCTATTTATGATTTTTACTTCTGGATGGACCCCTGAACATTGAATGGGGGAAAGGAAGGGTCGAAGGGATAAGTGGTATTTCGATTCTTGAGTTGATATCTGATAAATGATTATGCTTCTGGTTGCATATCGTGATTTTGCACAGATAGGTCGACTTGGAGGGAAGCACGATGGTTTAATAAAACCACACTGGGGAGGACCATGTGCCAGGATTACATTTTTCTTCTTAGCGAACTAGCTAAATTATGACTTTAACATTTAGGTCCTCTCTTTTGTATTTATTCATTGGGCATACGAGTAAATTATGTAGTCATTTTCTTCAATCGTTGGGCTTGCCTACATGTATTATTTTCCATGGGTAGCTCTAAGAGGTCACTAGTTGACTACTTCAATTACTAATTCATTACAATAACTGTTATCAGCCATTGTTTCACCAGTTGATGAAATTCTACAAAATTTAACTAAACACATTCGTAAATCAATTGAAAGCTCTTTGTATTTGAGTTTTCCATGTGGCTGATTGATTCTATTGGGCTTTGAGTTCTCTAGAATTTTACCCAACTCAAGTATTTGGCTGGTCTGGTTGTTACTTTATTGAAATTACTCACCTTCTCTCTGACCTAGTAATTGTTTGTTTCCTTCCATGCCTGTTTCATTTTGTGGTTGGTATTGAGTAATTACACTCTTGCAGGATGAGTTAATTGGGCATCTAACTACTTTGAAATAAAGTCGGTCACATGGTTGGTTGCTTCCACTTGGGTTTTAGGTATCTTAAATGTCTCGCCAACCTTCTGTTTTGGTAGTCTGTAAATGATCTATTGTTTTTTACTTTGTTCCCTATAATCATAATCTTATCTTCAAATTCTGTTTTTAATTACTGTTTCCCAAAATCTCTTCGGATGGGAAAATTTTCAGTCTAATTTGTTTTTGTGCGCTTTTCCAGGAAATATCTGCAGTTGCTGGACTAATCCATGGCCTTCGAGCAGTACTTTGCACAAGGATGGAAACATGTTTCTGGTACAGGGGGCACAGATCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATCTGCTTAGACTTTGCCCATGAGCCAGTGGTTACCCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGTGCCTCTCTTGCCATCGACGAGCACCCACAGTGCCCGGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTCTATGGTCGTGGCCAAACAGCCGAGGAAGTTGAGCCTGAAGCCAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAACCAAATGCTTGCTACTCCCAGCATACACCAACAGCAACTTCCATATCGAAATCCTTACCAGCAACCGAACCATGACCCCCTTCTTTTTGGCAATTATGAAGAAGATTCAGCAGCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCACCATCCATTGGTCGGAATGATTGGCGACTTTGTGCATGCGAGGGTATTTGGGAACTCGGATAACTTATACTCTTACCGAAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGGTTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAATTACATTCTATACCTGGAAACACCCCATTTTCAAGACAAGAAAGAAAGGAAGAAAGATAAAAATAAAATGCATTGTTGTCTTTGTATTCTCTGTATAGGCTTTTGGAGTTAAGCTCATGGACAATTATATGTTTATATATAATATATATACATATTGTTTA

mRNA sequence

GTCCACTAGGATGTTAAAAGAAAACGGAGGTTCGTCAATAAAACCCCAATCTCACGGGACGTTTTACGCTCCATCATTATCAACTCCTTCTTATCTCTGTTTCCCGTTCTGTCTTTTCGTTCTTCAACCTTTTCATTTTTGCATTAACATTGCCTCTTTTCTGTGGAACCAATCTCTTTCTCTCAGCGATCGCGCGCTCGGATTTTCTGATGGCATTTCTCGTTTCTTGGATCTTTCAGTCTGGATGAGTTTTTCTGATGAGTTAATTGGGCATCTAACTACTTTGAAATAAAGTCGGTCACATGGTTGGTTGCTTCCACTTGGGTTTTAGGAAATATCTGCAGTTGCTGGACTAATCCATGGCCTTCGAGCAGTACTTTGCACAAGGATGGAAACATGTTTCTGGTACAGGGGGCACAGATCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATCTGCTTAGACTTTGCCCATGAGCCAGTGGTTACCCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGTGCCTCTCTTGCCATCGACGAGCACCCACAGTGCCCGGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTCTATGGTCGTGGCCAAACAGCCGAGGAAGTTGAGCCTGAAGCCAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAACCAAATGCTTGCTACTCCCAGCATACACCAACAGCAACTTCCATATCGAAATCCTTACCAGCAACCGAACCATGACCCCCTTCTTTTTGGCAATTATGAAGAAGATTCAGCAGCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCACCATCCATTGGTCGGAATGATTGGCGACTTTGTGCATGCGAGGGTATTTGGGAACTCGGATAACTTATACTCTTACCGAAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGGTTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAATTACATTCTATACCTGGAAACACCCCATTTTCAAGACAAGAAAGAAAGGAAGAAAGATAAAAATAAAATGCATTGTTGTCTTTGTATTCTCTGTATAGGCTTTTGGAGTTAAGCTCATGGACAATTATATGTTTATATATAATATATATACATATTGTTTA

Coding sequence (CDS)

ATGGCCTTCGAGCAGTACTTTGCACAAGGATGGAAACATGTTTCTGGTACAGGGGGCACAGATCGAGAGAACTACAAGGGTTGTTTTGATTGTAACATCTGCTTAGACTTTGCCCATGAGCCAGTGGTTACCCTCTGTGGCCACTTATACTGCTGGCCTTGCATCTATAAATGGCTTCACGTCCAGAGTGCCTCTCTTGCCATCGACGAGCACCCACAGTGCCCGGTTTGCAAGGCTGATATTTCCCACACCACAATGGTTCCTCTCTATGGTCGTGGCCAAACAGCCGAGGAAGTTGAGCCTGAAGCCAAAGCAATGCATCATGACATTAACATACCTCCTAGACCTTCAGCTTGTGGTAACCAAATGCTTGCTACTCCCAGCATACACCAACAGCAACTTCCATATCGAAATCCTTACCAGCAACCGAACCATGACCCCCTTCTTTTTGGCAATTATGAAGAAGATTCAGCAGCCCCTTTGCTAAACCTTGCAAGAACATCATTCAGTGGCTTTCACCATCCATTGGTCGGAATGATTGGCGACTTTGTGCATGCGAGGGTATTTGGGAACTCGGATAACTTATACTCTTACCGAAACTCTTACCAGCTAACAGGGAGCAGTAGGAACCGGTTGAGAAGACAGGAAATGCAGGTAGACAAGTCATTGAACAGAATCTCCATTTTCCTCTTCTGCTGTGTAATTCTATGCGTTCTTGTCTTCTAA

Protein sequence

MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF

Homology

[Close]

BLAST of Bhi03G000323 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 178.7 bits (452), Expect = 5.5e-45
Identity = 100/227 (44.05%), Postives = 136/227 (59.91%), Query Frame = 0


Query: 24  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 83
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 84  HTTMVPLYGRGQTAEEVEPEAK---AMHHDINIPPRPSACGNQMLATPSI-----HQQQL 143
            T++VPLYGRG ++      +K   A+  DI   P PSA  N + +  S+     HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 144 PYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNSDN 203
           P  + +Q   + P  F   E        +LA      F +P++GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 204 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Bhi03G000323 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 7.0e-40
Identity = 87/216 (40.28%), Postives = 126/216 (58.33%), Query Frame = 0


Query: 29  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 88
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 89  VPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNPYQQPNHD 148
           VPLYGRG+   + E +       +    R     +   +T     Q++ + +P +     
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE----- 165

Query: 149 PLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 208
               G Y     + +++    S+S    P++ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 ----GYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 209 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi03G000323 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 7.0e-40
Identity = 87/216 (40.28%), Postives = 126/216 (58.33%), Query Frame = 0


Query: 29  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 88
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 89  VPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNPYQQPNHD 148
           VPLYGRG+   + E +       +    R     +   +T     Q++ + +P +     
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE----- 165

Query: 149 PLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 208
               G Y     + +++    S+S    P++ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 ----GYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 209 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi03G000323 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 126.3 bits (316), Expect = 3.3e-29
Identity = 75/223 (33.63%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 27  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 86
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 87  DISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNP 146
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 147 YQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFG-NSDNLYSY 206
           Y+ P+                              P++G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 207 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Bhi03G000323 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.2e-24
Identity = 49/101 (48.51%), Postives = 66/101 (65.35%), Query Frame = 0


Query: 17  TGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQCPV 76
           +G     N    F+CNICLD A +P+VTLCGHL+CWPC+YKWLH+ S S        CPV
Sbjct: 9   SGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQS------KDCPV 68

Query: 77  CKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPS 118
           CKA I    +VPLYGRG+++   +P +K++   + +P RPS
Sbjct: 69  CKAVIEEDRLVPLYGRGKSS--ADPRSKSI-PGLEVPNRPS 100

[Close]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.8e-48
Identity = 108/228 (47.37%), Postives = 142/228 (62.28%), Query Frame = 0


Query: 23  ENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASL--AIDEHPQCPVCKAD 82
           EN  G FDCNICLD  HEPV+TLCGHLYCWPCIYKW++ QS S   +  + PQCPVCKA+
Sbjct: 33  ENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQQQPQCPVCKAE 92

Query: 83  ISHTTMVPLYGRGQTAEEVEP-EAKAMHHDINIPPRPSA--CGNQ-MLATPSIHQQQLPY 142
           +S  T++PLYGRG   +  +P E KA +  I IP RP +  CG   +L T   +  QL  
Sbjct: 93  VSEKTLIPLYGRG--GQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTTDSNPSQLLQ 152

Query: 143 RNPYQQPN--HDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNSD- 202
           R  YQQ +    P   G+Y    ++P+L+    + +   H    MIG+  +AR+FGNS  
Sbjct: 153 RRGYQQQSQTRQPAYQGSY---MSSPMLSPGGATANMLQH---SMIGEVAYARIFGNSST 212

Query: 203 NLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
            +Y+Y NSY L  SS  R+RRQ  Q D+SL RI  FLFCC + C+++F
Sbjct: 213 TMYTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFVTCLILF 252

[Close]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 7.8e-44
Identity = 100/227 (44.05%), Postives = 136/227 (59.91%), Query Frame = 0


Query: 24  NYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHP-QCPVCKADIS 83
           N  GCFDCNICLD AH+PVVTLCGHL+CWPCIYKWLHVQ +S+++D+H   CPVCK++I+
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 84  HTTMVPLYGRGQTAEEVEPEAK---AMHHDINIPPRPSACGNQMLATPSI-----HQQQL 143
            T++VPLYGRG ++      +K   A+  DI   P PSA  N + +  S+     HQ   
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPAPSALRNPITSASSLNPSLQHQTLS 156

Query: 144 PYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNSDN 203
           P  + +Q   + P  F   E        +LA      F +P++GM GD V+ R+FG   N
Sbjct: 157 PSFHNHQ---YSPRGFTTTES------TDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTN 216

Query: 204 LYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
             +    YQ          ++ MQ +KSLNR+SIF  CC+ILC+L+F
Sbjct: 217 --TIAQPYQ---------SQRMMQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 9.9e-39
Identity = 87/216 (40.28%), Postives = 126/216 (58.33%), Query Frame = 0


Query: 29  FDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLA--IDEHPQCPVCKADISHTTM 88
           FDCNICLD   EPVVTLCGHL+CWPCI+KWL VQS S +     H QCPVCK+ +SH+T+
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 89  VPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNPYQQPNHD 148
           VPLYGRG+   + E +       +    R     +   +T     Q++ + +P +     
Sbjct: 106 VPLYGRGRCTTQEEGKNSVPKRPVGPVYRLEMPNSPYASTDLRLSQRVHFNSPQE----- 165

Query: 149 PLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFGNS-DNLYSYRNSYQLT 208
               G Y     + +++    S+S    P++ M+G+ V  R+FG    + ++Y ++Y L 
Sbjct: 166 ----GYY---PVSGVMSSNSLSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLA 225

Query: 209 GSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
           G+S  R+RR+ MQ DKSL RI  F  CCV+LC+L+F
Sbjct: 226 GTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.6e-28
Identity = 75/223 (33.63%), Postives = 114/223 (51.12%), Query Frame = 0


Query: 27  GCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSAS-LAIDEH------PQCPVCKA 86
           G FDCNICLD   +PVVTLCGHL+CWPCI+KW +  + S   +D++      P+CPVCK+
Sbjct: 17  GDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPVCKS 76

Query: 87  DISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACGNQMLATPSIHQQQLPYRNP 146
           D+S  T+VP+YGRGQ         KA     N+P RP+     +        +    R  
Sbjct: 77  DVSEATLVPIYGRGQ---------KAPQSGSNVPSRPTGPVYDLRGVGQRLGEGESQRYM 136

Query: 147 YQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMIGDFVHARVFG-NSDNLYSY 206
           Y+ P+                              P++G++ + V+ R+FG +S N+  Y
Sbjct: 137 YRMPD------------------------------PVMGVVCEMVYRRLFGESSSNMAPY 193

Query: 207 RNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLVF 242
           R+          R RR+ MQ ++SL+R+ +FL C + +C+ +F
Sbjct: 197 RD-------MNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.0e-19
Identity = 45/102 (44.12%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 15  SGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLHVQSASLAIDEHPQC 74
           S  G  D  N    F+CNICLD A + V++LCGHL+CWPC+++WL  +           C
Sbjct: 25  SSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETR------PNRQVC 84

Query: 75  PVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRP 117
           PVCKA IS   ++PLYGRG T ++ +P  K        PPRP
Sbjct: 85  PVCKAGISRDKVIPLYGRGSTGQQ-DPREKT-------PPRP 112

[Close]

BLAST of Bhi03G000323 vs. NCBI nr
Match: XP_038881993.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881995.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881996.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881997.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881998.1 E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 516.2 bits (1328), Expect = 1.6e-142
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI
Sbjct: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240

Query: 241 F 242
           F
Sbjct: 241 F 241

[Close]

BLAST of Bhi03G000323 vs. NCBI nr
Match: XP_004134707.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743485.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743486.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743487.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743488.1 E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >KGN49224.1 hypothetical protein Csa_003905 [Cucumis sativus])

HSP 1 Score: 488.4 bits (1256), Expect = 3.5e-134
Identity = 229/241 (95.02%), Postives = 234/241 (97.10%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAFEQYFAQGWKHVS   GT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE KAMHHDINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEEDSA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 241 F 242
           F
Sbjct: 241 F 238

[Close]

BLAST of Bhi03G000323 vs. NCBI nr
Match: XP_004134706.1 (E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743483.1 E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus])

HSP 1 Score: 488.4 bits (1256), Expect = 3.5e-134
Identity = 229/241 (95.02%), Postives = 234/241 (97.10%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAFEQYFAQGWKHVS   GT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 20  MAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 79

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE KAMHHDINIPPRPSACG
Sbjct: 80  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 139

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEEDSA+PLLNLARTSFS FHHP+VGMI
Sbjct: 140 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 199

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 200 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 257

Query: 241 F 242
           F
Sbjct: 260 F 257

[Close]

BLAST of Bhi03G000323 vs. NCBI nr
Match: XP_008439861.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa] >TYK13135.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo var. makuwa])

HSP 1 Score: 479.2 bits (1232), Expect = 2.1e-131
Identity = 226/241 (93.78%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAF+QYFAQGWKHVS   GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EAKAMH DINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEE SA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 241 F 242
           F
Sbjct: 241 F 238

[Close]

BLAST of Bhi03G000323 vs. NCBI nr
Match: XP_023003183.1 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003184.1 E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 458.8 bits (1179), Expect = 3.0e-125
Identity = 214/242 (88.43%), Postives = 227/242 (93.80%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MA+EQ FAQGWKHVSGTGGTDRE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHD-INIPPRPSAC 120
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PEA+A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 121 GNQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGM 180
           GNQ+LAT S HQQQL YRNPYQ+PNHDPLLFGNYEEDS AP LNLARTSFSGFHHP+VGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 181 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 240
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

Query: 241 VF 242
           VF
Sbjct: 241 VF 242

[Close]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL
Match: A0A0A0KK60 (Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.7e-134
Identity = 229/241 (95.02%), Postives = 234/241 (97.10%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAFEQYFAQGWKHVS   GT+RENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFEQYFAQGWKHVS---GTERENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPE KAMHHDINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEDKAMHHDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEEDSA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEDSASPLLNLARTSFSSFHHPVVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 241 F 242
           F
Sbjct: 241 F 238

[Close]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL
Match: A0A5A7UGP7 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007450 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.0e-131
Identity = 226/241 (93.78%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAF+QYFAQGWKHVS   GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EAKAMH DINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEE SA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 241 F 242
           F
Sbjct: 241 F 238

[Close]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL
Match: A0A1S3AZB8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.0e-131
Identity = 226/241 (93.78%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MAF+QYFAQGWKHVS   GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAFDQYFAQGWKHVS---GTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSACG 120
           VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEVE EAKAMH DINIPPRPSACG
Sbjct: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVELEAKAMHRDINIPPRPSACG 120

Query: 121 NQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGMI 180
           NQ+LATPS HQQQLPYRNPYQ+PNHDPLLF NYEE SA+PLLNLARTSFS FHHP+VGMI
Sbjct: 121 NQILATPSTHQQQLPYRNPYQRPNHDPLLFANYEEHSASPLLNLARTSFSSFHHPVVGMI 180

Query: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 240
           GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV
Sbjct: 181 GDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVLV 238

Query: 241 F 242
           F
Sbjct: 241 F 238

[Close]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL
Match: A0A6J1KVR4 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496874 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.4e-125
Identity = 214/242 (88.43%), Postives = 227/242 (93.80%), Query Frame = 0


Query: 1   MAFEQYFAQGWKHVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60
           MA+EQ FAQGWKHVSGTGGTDRE YKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH
Sbjct: 1   MAYEQCFAQGWKHVSGTGGTDRETYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWLH 60

Query: 61  VQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHD-INIPPRPSAC 120
           V+S SLAIDEHPQCPVCKADISHTTMVPLYGRGQ AEEV PEA+A+H D ++IPPRPSAC
Sbjct: 61  VKSVSLAIDEHPQCPVCKADISHTTMVPLYGRGQMAEEVGPEAQALHQDNVDIPPRPSAC 120

Query: 121 GNQMLATPSIHQQQLPYRNPYQQPNHDPLLFGNYEEDSAAPLLNLARTSFSGFHHPLVGM 180
           GNQ+LAT S HQQQL YRNPYQ+PNHDPLLFGNYEEDS AP LNLARTSFSGFHHP+VGM
Sbjct: 121 GNQILATLSTHQQQLRYRNPYQRPNHDPLLFGNYEEDSPAPSLNLARTSFSGFHHPVVGM 180

Query: 181 IGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVILCVL 240
           IGDF++ARVFGNSDNLYSY+NSY LTGS RNRLRRQEMQ +KSLNRISIFLFCCVILCVL
Sbjct: 181 IGDFMYARVFGNSDNLYSYQNSYHLTGSRRNRLRRQEMQAEKSLNRISIFLFCCVILCVL 240

Query: 241 VF 242
           VF
Sbjct: 241 VF 242

[Close]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL
Match: A0A6J1CKD0 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111012249 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 4.6e-124
Identity = 216/245 (88.16%), Postives = 226/245 (92.24%), Query Frame = 0


Query: 1   MAFEQYFAQGWK-HVSGTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 60
           MAFEQY+A GWK HVS TGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL
Sbjct: 1   MAFEQYYAHGWKQHVSSTGGTDRENYKGCFDCNICLDFAHEPVVTLCGHLYCWPCIYKWL 60

Query: 61  HVQSASLAIDEHPQCPVCKADISHTTMVPLYGRGQTAEEVEPEAKAMHHDINIPPRPSAC 120
           HVQSASLA DEHPQCPVCKADISHTTMVPLYGRGQ+AEEVEPEAKA+H DI+IPPRPSAC
Sbjct: 61  HVQSASLATDEHPQCPVCKADISHTTMVPLYGRGQSAEEVEPEAKAIHRDIDIPPRPSAC 120

Query: 121 GNQMLATPSI--HQQQLPYRNPYQQPNHDPLLFGNYEED-SAAPLLNLARTSFSGFHHPL 180
           GN MLATPSI   QQQLPYRNPYQ+PNHDPLLF NYEED SAAPLLNLARTSFS FHHP+
Sbjct: 121 GNHMLATPSIDHQQQQLPYRNPYQRPNHDPLLFSNYEEDSSAAPLLNLARTSFSNFHHPV 180

Query: 181 VGMIGDFVHARVFGNSDNLYSYRNSYQLTGSSRNRLRRQEMQVDKSLNRISIFLFCCVIL 240
           VGMIGD ++ RVFGNS+NLYSYRNSY +TGSS NRLRRQEMQ  KSLNRISIFL CCVIL
Sbjct: 181 VGMIGDLMYTRVFGNSENLYSYRNSYHVTGSSSNRLRRQEMQASKSLNRISIFLLCCVIL 240

Query: 241 CVLVF 242
           CVLVF
Sbjct: 241 CVLVF 245

The following BLAST results are available for this feature:

BLAST of Bhi03G000323 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	5.5e-45	44.05	RING membrane-anchor 3	[more]
AT4G03510.1	7.0e-40	40.28	RING membrane-anchor 1	[more]
AT4G03510.2	7.0e-40	40.28	RING membrane-anchor 1	[more]
AT4G28270.1	3.3e-29	33.63	RING membrane-anchor 2	[more]
AT1G19310.1	3.2e-24	48.51	RING/U-box superfamily protein	[more]

BLAST of Bhi03G000323 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	1.8e-48	47.37	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
Q8GUK7	7.8e-44	44.05	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
O64425	9.9e-39	40.28	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	4.6e-28	33.63	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q5ZIR9	1.0e-19	44.12	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]

BLAST of Bhi03G000323 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881993.1	1.6e-142	100.00	E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida] >XP_038881994.1 E3 u...	[more]
XP_004134707.1	3.5e-134	95.02	E3 ubiquitin-protein ligase RMA1H1 isoform X2 [Cucumis sativus] >XP_031743484.1 ...	[more]
XP_004134706.1	3.5e-134	95.02	E3 ubiquitin-protein ligase RMA1H1 isoform X1 [Cucumis sativus] >XP_031743482.1 ...	[more]
XP_008439861.1	2.1e-131	93.78	PREDICTED: E3 ubiquitin-protein ligase RMA1H1-like [Cucumis melo] >KAA0052689.1 ...	[more]
XP_023003183.1	3.0e-125	88.43	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 [Cucurbita maxima] >XP_023003...	[more]

BLAST of Bhi03G000323 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK60	1.7e-134	95.02	Rnf5 OS=Cucumis sativus OX=3659 GN=Csa_6G517320 PE=4 SV=1	[more]
A0A5A7UGP7	1.0e-131	93.78	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3AZB8	1.0e-131	93.78	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucumis melo OX=3656 GN=LOC103484523 ...	[more]
A0A6J1KVR4	1.4e-125	88.43	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CKD0	4.6e-124	88.16	E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 31..77 e-value: 1.2E-8 score: 44.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 31..78 score: 12.459772
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 31..77 e-value: 3.8E-8 score: 33.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 16..112 e-value: 8.7E-17 score: 63.0
None	No IPR available	PANTHER	PTHR12313:SF6	E3 UBIQUITIN-PROTEIN LIGASE RMA3	coord: 2..241
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 29..79 e-value: 2.22886E-25 score: 92.3671
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 25..92
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..241
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 46..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000323	Bhi03M000323	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity