Bhi03G000302 (gene) Wax gourd (B227) v1

Overview
NameBhi03G000302
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type domain-containing protein
Locationchr3: 7918154 .. 7922587 (-)
RNA-Seq ExpressionBhi03G000302
SyntenyBhi03G000302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGGCTCGGAAGCGCGCTTACGACACTGTGGATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGAAGATGGACAATCTGAGGGTACGCTTCTTGTTTCTCTTCAGCACCCTAAATTTTTCACGATGCAACCCACTCTTTGATGTTCCTGCATTTCATATATCTTATTATTTGTTTGTTTAATTCTTTTCTTTCTGCGTGTACTGAGTTCGTGCTGTTCTATGTTGCTTCTTCTTTCGCCAGAATCCGATCGAAGCCCTTCCTCCAGTCAAGGAGATAAGGACGAGTATGTTCTGTTTATCAGCTCTGTTTTTGGCTTACTTCTTTTTGTTCGTCATCTTTTTCGTGCCGTTCCTTTTTACTCTATTTACTCTTTTGATTTATTTCTTCTGCTTGGTTATGGAGTTATTTGGTGCCTAACTGCTTGGGATTGATTTGGGATCTGCATGCTATGCTTCGTGGTATTGTTCATTGAGGTCTTGCTGCGACCGTATTCGAAGACCAGCTGCGTAAGCTTCACGTGCGGAGGCATTCGTTGATTTTTGTCCTGCGCCGGAGTTTTCTTTACTTTCAAGTTTTCAGTTCCTTTTCCGATTAAGGTTAGTGGAATTGGGTGCTGGATCTTGTGGGTGAAGTGGGTTCTCGAGGTCGTATATGCGATCAATCAGTTTTTACCTATGCTACAAGCTGCTGGGTGGGTAATGACGTTGGAGCTCACATGGTTTTAGCTTCTCTACGATGTTAAAGCTCACATGATCCGAGATCAAGCTTTCACTCAACGTGGAATTAAGCTTACCATAACTGAAGCGCTCACGGAAAAATCTTGCTGGTTCCCGCCATTGATTGTTGGATCCAATTTTACGCAAATTCCAAGAAGTTAACGACTGGAAACCCGACATTTCATTAAGAACGAGTTTACCCAAAATTTTTACAACTTGAGCGCTACTCTTACGCTAGGGCGATGAACGAACTATTTGGGTTGCGTTATAGAATATACGCGTCTGAACAGAAAGTTGTTTATCCGTTCTTTAGTTGCATGGTGAACAGTAGAATTCCCTCAAGGATTAACACGATCTTAGGTGTCTTGCGATTATTTCGAATCCTAATTCTTCTGTCTGGTTTTCTTTTCCAGATTTATCGCCGTGAAATTGTCAGACATTCGGAAAGAAGTCCAATGTCCTATCTGCTTAGGTATGTTTTAATAATCCCTTCTTGTTGAACGTAGTTATCTCACATATGATAAGATTTCTATTGATTTAAACATTTGGTTTTAATTTTTATACAATTCATCTTCTGCAAGTACATAAACACGCTCACTACGTGAAACAATTTTGCTGTTTAATATTGGTGTCAACGTTTCATCTGGGTCTTTACTGACTTTAACAAGATTGTTGTCACATCCTGAGAATCAATCATCATATATAATTAACTTATTTTTGTTGGTTTATATACTTACCTATCTATAAGGTTATTTCAAATGATTTAACTGTCCGTGATGAAATGTGGAGGAGGAGACGATTAATTTTAAATGACGTGTTGAATCAAGTGCCACCGGACCCACCTGATCTAAGAATCTATTCTGATGCAGCAAAATTGATTTTTGTATATAAGGTGTGTCGGAGAACATAAATTTTTTTATTCTTTATAATCTCCATTCTCCGGATCTGGGAGTGATGGCAAGAACTAAAATTTGAGGTTGGCTTTGTATATGGACTATTGATTTAGAAATGGATCCCGATATGGTTTTTTTATCAGATTATGTACGCATTCCACAGTTTTAAGATGACAAACTTTTCCACCTCAACTTCTACTTTGTTATGGAGTTTTGCATTTTGTGATTACACCATTCCGTCAAGGTCACTAATTGGACTGTAGAGTGAGTGAATTCTTGAGCATATGAGGCTATGGAATTTGGTTTTCATTCCTTTTGGTGTTCTGTGTTCCTTTTCGATCCTTACCGTTTCAGGCTACATATAAGTACATTTAGGGCCAGTGGTTTGTGATGGTGTTAATGTTTACACGTAACAATTAGTACATTTATGCTCATATTGTCAAGACTCTTGATCTTTACTTTCAGTTCATGCACTTACAGCTAACACCAAAAAATTTTCCTTTTCATAACTAAATTCCAAATCAAAACATTCCTGAAATGTGACATTTTTTAGTGGTTGTATCCAAATACTATGAGCCATGACATTCTAAGTCTTAGCATGTTTGGGAAATATATTTAGGTGGGTTAGGTGAGGTAACCTCAGAGCTCACTTTGGAGGGTTATCTCCATGTTTATGCATGTATTCTTAAGGAGGTCGATAAAATTTTTTCAATTCCTAACGTGCAGGAATCATAAAAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCAATGCGCCTAGGGTATGCTCTTATGTAATTTTTATTTTGAAGAAAATAAATAAGTATCGTTGCACGATTTTCCTTCTGGCTTACACTAAACTTGTACTGAAATTTGCTGCAGTAATAAGGAGTGCCCCGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCAAATTATGATACCTTGATTGCCGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGTGGGAATGTTATGTTTTAACTTGCAAGCTTGGTGTTTGATGATTGATATTGTCATTTCTGTATACTCCTTGTTAATTTTTCTTTTTTGGTTAGGAACTGGCTTTCCAAGAAGAAGAGAAAGGTCGTAATAAGCAGGTGAGAAGGCTAAGTTCTGTGGATTTTTTTCGTTATGGACATGAATTTGTTTGTTTCCAGAGTTAATAAGCTCACTTTGCATTGTTAAATGTACAAGAAATCACAGAGTTTGTCATGCTGAAATTGATTAAGAGAGAGAGGAATTATTAAGTCATTATTTGATAAATATGTAATGCAAAATTGTGGAACATTGGGACATGAAATAGAGCATGTTTTTCTTCGCTGTTGTGCTTTTCTATTCCATTTAACTTGGAAAGATCTCGTGCTGAAAATCAAAATGGTTATTATAGAATTTACTTGTACCAGGTATCTAAGATTACACTCTATTATAGATTGTAGTTGTATCAGGTATCTATGATCACACTTGATCCTTTATCTTCACGTTTGGTTTAAAATGTTATTTTTGGGTTATTTTTCAGATCCAAACTTCCATCGCTCAGACACTTCAACGACAAACTGAAGCGTTGGGTAGGAAACGTTCTAAACCTTCGAGGAGATTAAGTTCTAGAGGATCCAAAAGCTTCCAAAATCATATTGAGAGTATATGCTTTGATGAAAACGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCGGATGAGCGAATGGACATTAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAACAGATGGTGGTATCGAAAATGATTATGAAGTGTACAAAGAAAAAGTTGGTGCATCTATAGGGCTTGTTGGTAGCTCGGAAAAACTTTCTTGGGGAAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTGTAAATGGGGGGGCTGGTAAAAATCCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGAAGGAGGTAAAAACAACAATTTGAGGTGTTACTGAACTTCCTTGTGAATAAATTATGGACATGTTAACGTATAAATGGCTCTCCTATTGAATCACATGCATGCTGTTATGCCTCTTTTAGCTGCTTAAAAGCTCAACGATCTTTTATGTTTCTATCCATCAATGACTTTTGCCTTCTCTTCCTGACTTTTAGTTGGACATCCACCTTGTGCTCATTTCCATCGATGAAACTAGAGTACCCGCATTGCAAAGACCATATATTCGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAAGTAATCTGTCACTAAATGGAGCTGCACTTCATCCGTTTATATTCTTAATTCATTTTTTAGTTGATCCTTGAGCTTCCTTTCTTCTGCAGTACGTTGCTCTCAAAACCGGGCTTTCCGTTGATGTTGTTGAAATGTGCGTGGCAAAAGAGCTGCAAATGAAACTCAATCCTTCAGACTCCGGAACTCTAAAGAACCCATGTAAAGAAATTATACAGATACTAAACGAGCAAGAAACGCTATCGACGGCCAAAGTCAAAGCACACAGGCTAGCTTGTGGTTATCTGGTATGCCTTGAATCTACAGCACACAAATGCTTTGAAAAAAAATGCACCCACTACATCAATAACCATTTGTATAAAGCCTTCTGTGCAACATTTTAAGCTTGTTCCCTTGATTCCAGCTCTTGGCATATAAGAAAAAGGGCTAG

mRNA sequence

ATGCCGGCTCGGAAGCGCGCTTACGACACTGTGGATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGAAGATGGACAATCTGAGGAATCCGATCGAAGCCCTTCCTCCAGTCAAGGAGATAAGGACGAATTTATCGCCGTGAAATTGTCAGACATTCGGAAAGAAGTCCAATGTCCTATCTGCTTAGGAATCATAAAAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCAATGCGCCTAGGTAATAAGGAGTGCCCCGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCAAATTATGATACCTTGATTGCCGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGAACTGGCTTTCCAAGAAGAAGAGAAAGGTCGTAATAAGCAGATCCAAACTTCCATCGCTCAGACACTTCAACGACAAACTGAAGCGTTGGGTAGGAAACGTTCTAAACCTTCGAGGAGATTAAGTTCTAGAGGATCCAAAAGCTTCCAAAATCATATTGAGAGTATATGCTTTGATGAAAACGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCGGATGAGCGAATGGACATTAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAACAGATGGTGGTATCGAAAATGATTATGAAGTGTACAAAGAAAAAGTTGGTGCATCTATAGGGCTTGTTGGTAGCTCGGAAAAACTTTCTTGGGGAAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTGTAAATGGGGGGGCTGGTAAAAATCCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGAAGGAGTTGGACATCCACCTTGTGCTCATTTCCATCGATGAAACTAGAGTACCCGCATTGCAAAGACCATATATTCGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATACGTTGCTCTCAAAACCGGGCTTTCCGTTGATGTTGTTGAAATGTGCGTGGCAAAAGAGCTGCAAATGAAACTCAATCCTTCAGACTCCGGAACTCTAAAGAACCCATGTAAAGAAATTATACAGATACTAAACGAGCAAGAAACGCTATCGACGGCCAAAGTCAAAGCACACAGGCTAGCTTGTGGTTATCTGCTCTTGGCATATAAGAAAAAGGGCTAG

Coding sequence (CDS)

ATGCCGGCTCGGAAGCGCGCTTACGACACTGTGGATGTTGATCTTTTGCCGCGACTCCATAATACCTTCAATGAATGCTCTGAAGATGGACAATCTGAGGAATCCGATCGAAGCCCTTCCTCCAGTCAAGGAGATAAGGACGAATTTATCGCCGTGAAATTGTCAGACATTCGGAAAGAAGTCCAATGTCCTATCTGCTTAGGAATCATAAAAAAAACCAGAACAGTGATGGAATGTCTGCACCGCTTCTGCAGGGAATGCATAGACAAATCAATGCGCCTAGGTAATAAGGAGTGCCCCGCTTGCCGCACTCATTGTGCCAGTCGACGCTCTCTGAGAGATGATCCAAATTATGATACCTTGATTGCCGTGTTATATCCAGATATTGACAAATATGAGGAAGAGGAACTGGCTTTCCAAGAAGAAGAGAAAGGTCGTAATAAGCAGATCCAAACTTCCATCGCTCAGACACTTCAACGACAAACTGAAGCGTTGGGTAGGAAACGTTCTAAACCTTCGAGGAGATTAAGTTCTAGAGGATCCAAAAGCTTCCAAAATCATATTGAGAGTATATGCTTTGATGAAAACGAGGACGAAAATGATTATGATGTCAGCAAAAATTCATCTTCTGCGGATGAGCGAATGGACATTAGACCTAAAAGATCCAGGAGAGGTGGTCCTGTTAGATTTTCCCAGTCTTCTTCAGCTACTGGAGCAGATGGAACAGATGGTGGTATCGAAAATGATTATGAAGTGTACAAAGAAAAAGTTGGTGCATCTATAGGGCTTGTTGGTAGCTCGGAAAAACTTTCTTGGGGAAAAGGTGGAATAAGAAGTCACACTAGATATGGGGGTGTAAATGGGGGGGCTGGTAAAAATCCCAGGAATAACCGCATCATGAAGCTGTCGGATTATGTTCGAAACTCAGAAAATGTCAGTGAGAAGGAGTTGGACATCCACCTTGTGCTCATTTCCATCGATGAAACTAGAGTACCCGCATTGCAAAGACCATATATTCGCTGCAGGCCTTCAGTGACGATTGGACACCTAAGTCAATACGTTGCTCTCAAAACCGGGCTTTCCGTTGATGTTGTTGAAATGTGCGTGGCAAAAGAGCTGCAAATGAAACTCAATCCTTCAGACTCCGGAACTCTAAAGAACCCATGTAAAGAAATTATACAGATACTAAACGAGCAAGAAACGCTATCGACGGCCAAAGTCAAAGCACACAGGCTAGCTTGTGGTTATCTGCTCTTGGCATATAAGAAAAAGGGCTAG

Protein sequence

MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYKKKG
Homology
BLAST of Bhi03G000302 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 332.4 bits (851), Expect = 5.3e-91
Identity = 196/422 (46.45%), Postives = 273/422 (64.69%), Query Frame = 0

Query: 25  ECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFC 84
           E  E+ + +  +RSPSS  GD+ EF+ + L +IRK+VQCPICLGIIKKTRTVMECLHRFC
Sbjct: 98  EEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFC 157

Query: 85  RECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEK 144
           RECIDKSMRLGN ECPACR HCASRRSLRDDP +D LIA L+ +ID YEEEELAF E+E 
Sbjct: 158 RECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEM 217

Query: 145 GRNKQIQTSIAQTLQRQTEALGRKRS---------------KPSRRLSSRGSKSFQNHIE 204
            RNKQIQ SIAQ  QRQ+EAL ++RS                 SRR  +  +   QN  E
Sbjct: 218 ARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASE 277

Query: 205 SICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGG-IEN 264
           +   D+N+D N+    K+SSS +   ++R K+ R+    R +Q  S++GA+  +G   +N
Sbjct: 278 AHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADN 337

Query: 265 DYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYG-GVNGGAGKNPRNNRIMKLSDYVR 324
           D EVY++  G S GLV + E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R
Sbjct: 338 DTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLR 397

Query: 325 NSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVDVVEM 384
           +S + S  ELDIH+ L+S+D   +P L +PY+ CRP++ +  L ++VAL+  L  + VE+
Sbjct: 398 SSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVEL 457

Query: 385 CVAK-----ELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYK 425
            V +     +  ++  P+ +       K+ +  L + ETLS  K+        +L++AY+
Sbjct: 458 LVTRRRVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISSHEQHLIIAYR 517

BLAST of Bhi03G000302 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 265.8 bits (678), Expect = 6.1e-71
Identity = 172/426 (40.38%), Postives = 256/426 (60.09%), Query Frame = 0

Query: 25  ECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFC 84
           E  E+ + +  +RSPSS  GD+ EF+ + L +IRK+VQCPICL  +K  R  +   ++  
Sbjct: 98  EEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLVGMKFLRLFIS-YYKEN 157

Query: 85  RECIDKSMR----LGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQ 144
           ++C   S +      N ECPACR HCASRRSLRDDP +D LIA L+ +ID YEEEELAF 
Sbjct: 158 KDCDGMSPQFCATFKNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFH 217

Query: 145 EEEKGRNKQIQTSIAQTLQRQTEALGRKRS---------------KPSRRLSSRGSKSFQ 204
           E+E  RNKQIQ SIAQ  QRQ+EAL ++RS                 SRR  +  +   Q
Sbjct: 218 EDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQ 277

Query: 205 NHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGG 264
           N  E+   D+N+D N+    K+SSS +   ++R K+ R+    R +Q  S++GA+  +G 
Sbjct: 278 NASEAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGN 337

Query: 265 -IENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYG-GVNGGAGKNPRNNRIMKLS 324
             +ND EVY++  G S GLV + E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL 
Sbjct: 338 CADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLV 397

Query: 325 DYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVD 384
           +Y+R+S + S  ELDIH+ L+S+D   +P L +PY+ CRP++ +  L ++VAL+  L  +
Sbjct: 398 EYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTE 457

Query: 385 VVEMCVAK-----ELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 425
            VE+ V +     +  ++  P+ +       K+ +  L + ETLS  K+        +L+
Sbjct: 458 EVELLVTRRRVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISSHEQHLI 517

BLAST of Bhi03G000302 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-70
Identity = 178/408 (43.63%), Postives = 252/408 (61.76%), Query Frame = 0

Query: 30  GQSEES-DRSPSSSQGD--KDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRE 89
           G+ E+S +RS SSS G+  + E++ V L+DI K+VQC ICLGII+KTRTVMECLHRFCRE
Sbjct: 67  GEEEDSKERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRE 126

Query: 90  CIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEKGR 149
           CIDKSMRLGN ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEEEL F+++++ R
Sbjct: 127 CIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEAR 186

Query: 150 NKQIQTSIAQTLQRQTEALGRKRS--------KPSRRLSSRGSKSFQN--HIESICFDEN 209
           NKQIQ SIAQ  QRQ++AL +++S          SRR S  GS+  +N  +IE    + N
Sbjct: 187 NKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRR-SGGGSRRRRNCRNIEQDTSEAN 246

Query: 210 EDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKEK 269
           +D++     K+SSS +     R KRS        + ++     +GT+   + D  V    
Sbjct: 247 DDDDQNKRGKDSSSDEPCERQRKKRSATQPSSSNANNNDNCAGNGTEQTHQRDSRV---- 306

Query: 270 VGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKLSDYVRNSENVSEKE 329
              S  LV +SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  E
Sbjct: 307 --ISPVLVWNSELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVE 366

Query: 330 LDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVDVVEMCVAKELQMK 389
           LDIHL L+S+D   +  L  PY+  RP++ +  L +YVA    L  + VE+ V+K+    
Sbjct: 367 LDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKD---- 426

Query: 390 LNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYKKK 425
               D+        E +Q L + ET++  KV     + GY+++ Y++K
Sbjct: 427 ---GDTVIGNKTSTEKMQSLQDDETVAKLKVDCIS-SNGYMIVVYRRK 457

BLAST of Bhi03G000302 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-68
Identity = 178/414 (43.00%), Postives = 252/414 (60.87%), Query Frame = 0

Query: 30  GQSEES-DRSPSSSQGD--KDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRE 89
           G+ E+S +RS SSS G+  + E++ V L+DI K+VQC ICLGII+KTRTVMECLHRFCRE
Sbjct: 67  GEEEDSKERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRE 126

Query: 90  CIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEKGR 149
           CIDKSMRLGN ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEEEL F+++++ R
Sbjct: 127 CIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEAR 186

Query: 150 NKQIQTSIAQTLQRQTEALGRKRS--------KPSRRLSSRGSKSFQN--HIESICFDEN 209
           NKQIQ SIAQ  QRQ++AL +++S          SRR S  GS+  +N  +IE    + N
Sbjct: 187 NKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRR-SGGGSRRRRNCRNIEQDTSEAN 246

Query: 210 EDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKEK 269
           +D++     K+SSS +     R KRS        + ++     +GT+   + D  V    
Sbjct: 247 DDDDQNKRGKDSSSDEPCERQRKKRSATQPSSSNANNNDNCAGNGTEQTHQRDSRV---- 306

Query: 270 VGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKLSDYVRNSENVSEKE 329
              S  LV +SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  E
Sbjct: 307 --ISPVLVWNSELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVE 366

Query: 330 LDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQ------YVALKTGLSVDVVEMCVA 389
           LDIHL L+S+D   +  L  PY+  RP++ +  L +      YVA    L  + VE+ V+
Sbjct: 367 LDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLVS 426

Query: 390 KELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYKKK 425
           K+        D+        E +Q L + ET++  KV     + GY+++ Y++K
Sbjct: 427 KD-------GDTVIGNKTSTEKMQSLQDDETVAKLKVDCIS-SNGYMIVVYRRK 463

BLAST of Bhi03G000302 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-68
Identity = 177/415 (42.65%), Postives = 251/415 (60.48%), Query Frame = 0

Query: 28  EDGQSEESDRSPSSSQGD--KDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCR 87
           E+  S+  +RS SSS G+  + E++ V L+DI K+VQC ICLGII+KTRTVMECLHRFCR
Sbjct: 68  EEEVSDSKERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCR 127

Query: 88  ECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEKG 147
           ECIDKSMRLGN ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEEEL F+++++ 
Sbjct: 128 ECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEA 187

Query: 148 RNKQIQTSIAQTLQRQTEALGRKRS--------KPSRRLSSRGSKSFQN--HIESICFDE 207
           RNKQIQ SIAQ  QRQ++AL +++S          SRR S  GS+  +N  +IE    + 
Sbjct: 188 RNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRR-SGGGSRRRRNCRNIEQDTSEA 247

Query: 208 NEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKE 267
           N+D++     K+SSS +     R KRS        + ++     +GT+   + D  V   
Sbjct: 248 NDDDDQNKRGKDSSSDEPCERQRKKRSATQPSSSNANNNDNCAGNGTEQTHQRDSRV--- 307

Query: 268 KVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKLSDYVRNSENVSEK 327
               S  LV +SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  
Sbjct: 308 ---ISPVLVWNSELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSV 367

Query: 328 ELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQ------YVALKTGLSVDVVEMCV 387
           ELDIHL L+S+D   +  L  PY+  RP++ +  L +      YVA    L  + VE+ V
Sbjct: 368 ELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVELLV 427

Query: 388 AKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYKKK 425
           +K+        D+        E +Q L + ET++  KV     + GY+++ Y++K
Sbjct: 428 SKD-------GDTVIGNKTSTEKMQSLQDDETVAKLKVDCIS-SNGYMIVVYRRK 465

BLAST of Bhi03G000302 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 332.4 bits (851), Expect = 7.4e-90
Identity = 196/422 (46.45%), Postives = 273/422 (64.69%), Query Frame = 0

Query: 25  ECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFC 84
           E  E+ + +  +RSPSS  GD+ EF+ + L +IRK+VQCPICLGIIKKTRTVMECLHRFC
Sbjct: 98  EEEEEEEEDSKERSPSSISGDQSEFMEIDLGEIRKDVQCPICLGIIKKTRTVMECLHRFC 157

Query: 85  RECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEK 144
           RECIDKSMRLGN ECPACR HCASRRSLRDDP +D LIA L+ +ID YEEEELAF E+E 
Sbjct: 158 RECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEM 217

Query: 145 GRNKQIQTSIAQTLQRQTEALGRKRS---------------KPSRRLSSRGSKSFQNHIE 204
            RNKQIQ SIAQ  QRQ+EAL ++RS                 SRR  +  +   QN  E
Sbjct: 218 ARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNASE 277

Query: 205 SICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGG-IEN 264
           +   D+N+D N+    K+SSS +   ++R K+ R+    R +Q  S++GA+  +G   +N
Sbjct: 278 AHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADN 337

Query: 265 DYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYG-GVNGGAGKNPRNNRIMKLSDYVR 324
           D EVY++  G S GLV + E L+WG+GG RS+TR+G   +GG+ K+ RN R+ KL +Y+R
Sbjct: 338 DTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLR 397

Query: 325 NSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVDVVEM 384
           +S + S  ELDIH+ L+S+D   +P L +PY+ CRP++ +  L ++VAL+  L  + VE+
Sbjct: 398 SSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVEL 457

Query: 385 CVAK-----ELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYK 425
            V +     +  ++  P+ +       K+ +  L + ETLS  K+        +L++AY+
Sbjct: 458 LVTRRRVGEDAAIENLPAVTPASAAASKDEMLSLEDNETLSRLKIDFISSHEQHLIIAYR 517

BLAST of Bhi03G000302 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.9e-69
Identity = 178/408 (43.63%), Postives = 252/408 (61.76%), Query Frame = 0

Query: 30  GQSEES-DRSPSSSQGD--KDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRE 89
           G+ E+S +RS SSS G+  + E++ V L+DI K+VQC ICLGII+KTRTVMECLHRFCRE
Sbjct: 67  GEEEDSKERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRE 126

Query: 90  CIDKSMRLGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEELAFQEEEKGR 149
           CIDKSMRLGN ECP CR HCASRRSLRDDPN+D LIA L+ +IDK+EEEEL F+++++ R
Sbjct: 127 CIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEAR 186

Query: 150 NKQIQTSIAQTLQRQTEALGRKRS--------KPSRRLSSRGSKSFQN--HIESICFDEN 209
           NKQIQ SIAQ  QRQ++AL +++S          SRR S  GS+  +N  +IE    + N
Sbjct: 187 NKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRR-SGGGSRRRRNCRNIEQDTSEAN 246

Query: 210 EDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKEK 269
           +D++     K+SSS +     R KRS        + ++     +GT+   + D  V    
Sbjct: 247 DDDDQNKRGKDSSSDEPCERQRKKRSATQPSSSNANNNDNCAGNGTEQTHQRDSRV---- 306

Query: 270 VGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIMKLSDYVRNSENVSEKE 329
              S  LV +SE ++WG+GG RS+TR G  N GA  + RN R+ +L +Y+ + E  +  E
Sbjct: 307 --ISPVLVWNSELIAWGRGGTRSNTRQGNNNQGA-ISKRNARLKRLVEYLGSLEG-NSVE 366

Query: 330 LDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGLSVDVVEMCVAKELQMK 389
           LDIHL L+S+D   +  L  PY+  RP++ +  L +YVA    L  + VE+ V+K+    
Sbjct: 367 LDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKD---- 426

Query: 390 LNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLAYKKK 425
               D+        E +Q L + ET++  KV     + GY+++ Y++K
Sbjct: 427 ---GDTVIGNKTSTEKMQSLQDDETVAKLKVDCIS-SNGYMIVVYRRK 457

BLAST of Bhi03G000302 vs. ExPASy Swiss-Prot
Match: A1YER5 (E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.3e-21
Identity = 96/315 (30.48%), Postives = 139/315 (44.13%), Query Frame = 0

Query: 50  IAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASR 109
           IAV    +  E+ CPICL ++K T T  ECLHRFC +CI  ++R GNKECP CR    S+
Sbjct: 6   IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 65

Query: 110 RSLRDDPNYDTLIAVLYPDIDKYE--EEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGR 169
           RSLR DPN+D LI+ +YP  ++YE  ++ +  +       + + +SI + L+ Q     +
Sbjct: 66  RSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQ 125

Query: 170 KRSKPSRRLSSRGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGP 229
           +  +P   +      +  +  E    D  E E D +   + S+ D      PKR R GG 
Sbjct: 126 RVRRP---IPGSDQTTTMSGGEG---DPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGA 185

Query: 230 VRFSQSSSATGADGTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVN 289
                SS  TG  GT G            VG   G   S ++              GG  
Sbjct: 186 ---GGSSVGTGGGGTGG------------VGGGAGSEDSGDR--------------GGTL 245

Query: 290 GGAGKNPRNNRIMKLSDYVRNSENVSEKELDI--HLVLISIDETRVPALQRPYIRCRPSV 349
           GG    P +             E   E EL    H +L+   E      Q  Y++   + 
Sbjct: 246 GGGTLGPPSP-----PGAPSPPEPGGEIELVFRPHPLLVEKGE----YCQTRYVKTTGNA 276

Query: 350 TIGHLSQYVALKTGL 361
           T+ HLS+Y+AL+  L
Sbjct: 306 TVDHLSKYLALRIAL 276

BLAST of Bhi03G000302 vs. ExPASy Swiss-Prot
Match: Q66J69 (E3 ubiquitin-protein ligase RING2-A OS=Xenopus laevis OX=8355 GN=rnf2-a PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.3e-21
Identity = 85/252 (33.73%), Postives = 125/252 (49.60%), Query Frame = 0

Query: 34  ESDRSPSSSQGDKDEFIAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMR 93
           E  R+P  +  D  E IAV    +  E+ CPICL ++K T T  ECLHRFC +CI  ++R
Sbjct: 20  ELHRTPQEAIMDGTE-IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALR 79

Query: 94  LGNKECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYE-EEELAFQEEEKGRNKQ-IQ 153
            GNKECP CR    S+RSLR DPN+D LI+ +YP  D+YE  ++    +  +  N+Q + 
Sbjct: 80  SGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQDRVLAKLNRLHNQQALS 139

Query: 154 TSIAQTLQRQTEALGRKRSKPSRRLSSRGSKSFQNHIESICFDENEDENDYDVSKNSSSA 213
           +SI + L+ Q  A+ R          ++  +  Q+  ++  F   ED  D     ++ S 
Sbjct: 140 SSIEEGLKMQ--AMHR----------AQRVRKHQHESDNTTFSGGEDNCDSRSHVSNPSV 199

Query: 214 DERMDIRPKRSRRGGPVRFSQSSSATGADGTDGGIENDYEVYKEKVGASIGLVGSSEKLS 273
               +  P R R     R S+ S A      +GG+ +       + G+ I LV  +  L 
Sbjct: 200 HSNQEAGPSRKRS----RASEDSGAEPDLSHEGGVRSPDPPGGGETGSEIELVFRAHPLL 253

Query: 274 WGKGGIRSHTRY 284
             K G  S TRY
Sbjct: 260 VEKDGY-SQTRY 253

BLAST of Bhi03G000302 vs. ExPASy Swiss-Prot
Match: Q7ZWM8 (E3 ubiquitin-protein ligase RING2-B OS=Xenopus laevis OX=8355 GN=rnf2-b PE=2 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 4.3e-21
Identity = 80/236 (33.90%), Postives = 118/236 (50.00%), Query Frame = 0

Query: 50  IAVKLSDIRKEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASR 109
           IAV    +  E+ CPICL ++K T T  ECLHRFC +CI  ++R GNKECP CR    S+
Sbjct: 34  IAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSK 93

Query: 110 RSLRDDPNYDTLIAVLYPDIDKYE-EEELAFQEEEKGRNKQ-IQTSIAQTLQRQTEALGR 169
           RSLR DPN+D LI+ +YP  D+YE  ++    +  +  N+Q + +SI + L+ Q  A+ R
Sbjct: 94  RSLRPDPNFDALISKIYPSRDEYEAHQDRVLAKLSRLHNQQALSSSIEEGLKMQ--AMHR 153

Query: 170 KRSKPSRRLSSRGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGP 229
                     ++  +  Q+  ++  F   ED  D     ++ S     +  P R R    
Sbjct: 154 ----------AQRVRKHQHESDNTTFSGGEDNCDSRSHVSNPSVHSNQEAGPSRKRS--- 213

Query: 230 VRFSQSSSATGADGTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRY 284
            R S+ S A      +GG+ +       + G+ I LV  +  L   K G  S TRY
Sbjct: 214 -RASEDSGAEPDLSHEGGVRSPDPPGGGENGSEIELVFRAHPLLVEKDGY-SQTRY 252

BLAST of Bhi03G000302 vs. NCBI nr
Match: XP_038881714.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida])

HSP 1 Score: 829.3 bits (2141), Expect = 1.5e-236
Identity = 425/425 (100.00%), Postives = 425/425 (100.00%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE 60
           MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE
Sbjct: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE 60

Query: 61  VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT 120
           VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT
Sbjct: 61  VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT 120

Query: 121 LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG 180
           LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG
Sbjct: 121 LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG 180

Query: 181 SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGAD 240
           SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGAD
Sbjct: 181 SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATGAD 240

Query: 241 GTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIM 300
           GTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIM
Sbjct: 241 GTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRIM 300

Query: 301 KLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGL 360
           KLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGL
Sbjct: 301 KLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKTGL 360

Query: 361 SVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLA 420
           SVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLA
Sbjct: 361 SVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLLLA 420

Query: 421 YKKKG 426
           YKKKG
Sbjct: 421 YKKKG 425

BLAST of Bhi03G000302 vs. NCBI nr
Match: XP_004134950.1 (putative E3 ubiquitin-protein ligase RING1a [Cucumis sativus] >KGN49242.1 hypothetical protein Csa_003957 [Cucumis sativus])

HSP 1 Score: 721.1 bits (1860), Expect = 5.7e-204
Identity = 378/428 (88.32%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RR GPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRCGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE++YEV KEKVGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGK  RNN
Sbjct: 241 ADGADGGGIEHEYEVNKEKVGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKISRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D T +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRT-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVAKELQ+KL+PS S   KNPCKE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TSLSVDVVEICVAKELQVKLDPSTSEATKNPCKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 427

BLAST of Bhi03G000302 vs. NCBI nr
Match: KAA0052674.1 (putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa])

HSP 1 Score: 709.5 bits (1830), Expect = 1.7e-200
Identity = 374/428 (87.38%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RRGGPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRGGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE+DY      VGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGKN RNN
Sbjct: 241 ADGADVGGIEHDY------VGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKNSRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D + +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRS-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVA ELQMKL+PS S  +KNP KE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TALSVDVVEICVANELQMKLDPSTSEAIKNPSKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 421

BLAST of Bhi03G000302 vs. NCBI nr
Match: XP_008439830.1 (PREDICTED: putative E3 ubiquitin-protein ligase RING1a [Cucumis melo])

HSP 1 Score: 708.0 bits (1826), Expect = 5.0e-200
Identity = 373/428 (87.15%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RRGGPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRGGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE++Y      VGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGKN RNN
Sbjct: 241 ADGADVGGIEHEY------VGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKNSRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D + +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRS-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVA ELQMKL+PS S  +KNP KE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TALSVDVVEICVANELQMKLDPSTSEAIKNPSKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 421

BLAST of Bhi03G000302 vs. NCBI nr
Match: XP_023543674.1 (putative E3 ubiquitin-protein ligase RING1a isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695.3 bits (1793), Expect = 3.3e-196
Identity = 357/427 (83.61%), Postives = 388/427 (90.87%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE 60
           MPARKR Y+TV  DLLPRLH+TFN+ SEDG+SEESDRS SS Q DKDEF+AVKLSDIR++
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSGQEDKDEFVAVKLSDIRRD 60

Query: 61  VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT 120
           VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPN+DT
Sbjct: 61  VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDT 120

Query: 121 LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG 180
           LIAVLYPDIDKYEEEELAFQEEEK RNKQIQ+SIAQTL+RQTEALGRKRSKP RRLSSRG
Sbjct: 121 LIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRG 180

Query: 181 SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRP-KRSRRGGPVRFSQSSSATGA 240
            KSFQNHIES+ FDENEDENDYD SKNSSSADERMD RP KRSRRGGPVRFSQSSSATGA
Sbjct: 181 RKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGA 240

Query: 241 DGTDGGI-ENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNR 300
           DG DGG  ENDYEV KE+VGAS+GLV SSEKLSWGKGGIRSHTRYGG+NGG GK  RNNR
Sbjct: 241 DGADGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNR 300

Query: 301 IMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKT 360
           I KLSDYVRNSEN+SE+ELD+HL+L+S+D+TR+PAL+ PYI CRPSVTIGHL Q VAL+T
Sbjct: 301 ITKLSDYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALET 360

Query: 361 GLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 420
            LSVDV+EM V KELQMK+NPS SGT  +PCKE  QILN+QETLS  K+K HRLACGYLL
Sbjct: 361 ALSVDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

Query: 421 LAYKKKG 426
           LAY+KKG
Sbjct: 421 LAYQKKG 427

BLAST of Bhi03G000302 vs. ExPASy TrEMBL
Match: A0A0A0KLI2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.7e-204
Identity = 378/428 (88.32%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RR GPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRCGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE++YEV KEKVGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGK  RNN
Sbjct: 241 ADGADGGGIEHEYEVNKEKVGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKISRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D T +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRT-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVAKELQ+KL+PS S   KNPCKE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TSLSVDVVEICVAKELQVKLDPSTSEATKNPCKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 427

BLAST of Bhi03G000302 vs. ExPASy TrEMBL
Match: A0A5A7UBC4 (Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002860 PE=4 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 8.3e-201
Identity = 374/428 (87.38%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RRGGPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRGGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE+DY      VGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGKN RNN
Sbjct: 241 ADGADVGGIEHDY------VGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKNSRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D + +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRS-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVA ELQMKL+PS S  +KNP KE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TALSVDVVEICVANELQMKLDPSTSEAIKNPSKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 421

BLAST of Bhi03G000302 vs. ExPASy TrEMBL
Match: A0A1S3AZN3 (putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo OX=3656 GN=LOC103484507 PE=4 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.4e-200
Identity = 373/428 (87.15%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLL-PRLHNTF-NECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIR 60
           MPARKRAYD++DVDLL PRLHNTF NE S+D QS+ESDRS SSSQGD+DEFIAVKLSDIR
Sbjct: 1   MPARKRAYDSLDVDLLPPRLHNTFNNERSQDEQSQESDRSSSSSQGDEDEFIAVKLSDIR 60

Query: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120
           KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY
Sbjct: 61  KEVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNY 120

Query: 121 DTLIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSS 180
           DTLIAVLYPDI+KYEEEELAFQEEEK RNKQIQTSIAQTLQRQTE+LGRKRSKPSRRLSS
Sbjct: 121 DTLIAVLYPDIEKYEEEELAFQEEEKARNKQIQTSIAQTLQRQTESLGRKRSKPSRRLSS 180

Query: 181 RGSKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRPKRSRRGGPVRFSQSSSATG 240
           RG KSFQNHIES+CFDENEDENDYDVSKNSSSADERMD RPKR RRGGPVRFSQSSSATG
Sbjct: 181 RGPKSFQNHIESLCFDENEDENDYDVSKNSSSADERMDTRPKRPRRGGPVRFSQSSSATG 240

Query: 241 ADGTD-GGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNN 300
           ADG D GGIE++Y      VGAS+GLVGSSEKLSWGKGGIRSHTRYGG NGGAGKN RNN
Sbjct: 241 ADGADVGGIEHEY------VGASLGLVGSSEKLSWGKGGIRSHTRYGGTNGGAGKNSRNN 300

Query: 301 RIMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALK 360
           RI KLSDYVRNSENVSE+ELDIH++L+S+D + +PALQRPYI CRPSV IGHLSQYVAL+
Sbjct: 301 RIAKLSDYVRNSENVSEEELDIHVLLVSMDRS-IPALQRPYICCRPSVMIGHLSQYVALE 360

Query: 361 TGLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYL 420
           T LSVDVVE+CVA ELQMKL+PS S  +KNP KE +QILNEQETLSTAK+KAHRLACGYL
Sbjct: 361 TALSVDVVEICVANELQMKLDPSTSEAIKNPSKESVQILNEQETLSTAKLKAHRLACGYL 420

Query: 421 LLAYKKKG 426
           LLAYKKKG
Sbjct: 421 LLAYKKKG 421

BLAST of Bhi03G000302 vs. ExPASy TrEMBL
Match: A0A6J1ECT6 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.0e-195
Identity = 356/427 (83.37%), Postives = 387/427 (90.63%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE 60
           MPARKR Y+TV  DLLPRLH+TFN+ SEDG+SEESDRS SSSQ DKDEF+AVKLSDIR++
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDKDEFVAVKLSDIRRD 60

Query: 61  VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT 120
           VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPN+DT
Sbjct: 61  VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDT 120

Query: 121 LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG 180
           LIAVLYPDIDKYEEEELAFQEEEK RNKQIQ+SIAQTL+RQTEALGRKRSKP RRLSSRG
Sbjct: 121 LIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRG 180

Query: 181 SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRP-KRSRRGGPVRFSQSSSATGA 240
            KSFQNHIES+ FDENEDENDYD SKNSSSADERMD RP KRSRRGGPVRFSQSSSATGA
Sbjct: 181 RKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGA 240

Query: 241 DGTDGGI-ENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNR 300
           DG DGG  ENDYEV KE+VGAS+GLV SSEKLSWGKGGIRSHTRYGG+NGG GK  RNNR
Sbjct: 241 DGADGGSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNR 300

Query: 301 IMKLSDYVRNSENVSEKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKT 360
           I KLSDYVRNSEN+SE+ELD+ L+L+S+D+TR+PAL+  YI CRPSVTIGHL Q VAL+T
Sbjct: 301 ITKLSDYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALET 360

Query: 361 GLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 420
            LSVDV+EM V KELQMK+NPS SGT  +PCKE  QILN+QETLS  K+K HRLACGYLL
Sbjct: 361 ALSVDVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

Query: 421 LAYKKKG 426
           LAY+KKG
Sbjct: 421 LAYQKKG 427

BLAST of Bhi03G000302 vs. ExPASy TrEMBL
Match: A0A6J1INR9 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 7.0e-192
Identity = 354/427 (82.90%), Postives = 384/427 (89.93%), Query Frame = 0

Query: 1   MPARKRAYDTVDVDLLPRLHNTFNECSEDGQSEESDRSPSSSQGDKDEFIAVKLSDIRKE 60
           MPARKR Y+TV  DLLPRLH+TFN+ SEDG+SEESDRS SSSQ D+DEF+AVKLSDIR++
Sbjct: 1   MPARKRVYETVHADLLPRLHDTFNDRSEDGESEESDRSSSSSQEDEDEFVAVKLSDIRRD 60

Query: 61  VQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNKECPACRTHCASRRSLRDDPNYDT 120
           VQCPICLGII+KTRTVMECLHRFCRECIDKSMRLGN ECPACRTHCASRRSLRDDPN+DT
Sbjct: 61  VQCPICLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDT 120

Query: 121 LIAVLYPDIDKYEEEELAFQEEEKGRNKQIQTSIAQTLQRQTEALGRKRSKPSRRLSSRG 180
           LIAVLYPDIDKYEEEELAFQEEEK RNKQIQ+SIAQTL+RQTEALGRKRSKP RRLSSRG
Sbjct: 121 LIAVLYPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRG 180

Query: 181 SKSFQNHIESICFDENEDENDYDVSKNSSSADERMDIRP-KRSRRGGPVRFSQSSSATGA 240
            KSFQNHIES+ FDENEDENDYD SKNSSSADERMD RP KRSRRGGPVRFSQSSSATGA
Sbjct: 181 QKSFQNHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGA 240

Query: 241 DGTDGGIENDYEVYKEKVGASIGLVGSSEKLSWGKGGIRSHTRYGGVNGGAGKNPRNNRI 300
           DG  GG END EV KEKVGAS+GLV SSEK SWGKGGIRSHTRYGG+NGG GK  RNNRI
Sbjct: 241 DG--GGNENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRI 300

Query: 301 MKLSDYVRNSENVS-EKELDIHLVLISIDETRVPALQRPYIRCRPSVTIGHLSQYVALKT 360
            KLSDYVRNSEN+S E+ELD+HL+L+S+D+TR+PAL+ PYI CRPSVTIGHL Q VAL T
Sbjct: 301 TKLSDYVRNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVT 360

Query: 361 GLSVDVVEMCVAKELQMKLNPSDSGTLKNPCKEIIQILNEQETLSTAKVKAHRLACGYLL 420
            LSVDV+EM V KELQMK+NPS SG   +PCKE  QILN+QETLS  K+K HRLACGYLL
Sbjct: 361 ALSVDVIEMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLL 420

Query: 421 LAYKKKG 426
           LAY+KKG
Sbjct: 421 LAYQKKG 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G44280.15.3e-9146.45RING 1A [more]
AT5G44280.26.1e-7140.38RING 1A [more]
AT1G03770.11.4e-7043.63RING 1B [more]
AT1G03770.31.3e-6843.00RING 1B [more]
AT1G03770.21.3e-6842.65RING 1B [more]
Match NameE-valueIdentityDescription
Q9FKW07.4e-9046.45Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q0WX001.9e-6943.63Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=R... [more]
A1YER53.3e-2130.48E3 ubiquitin-protein ligase RING1 OS=Gorilla gorilla gorilla OX=9595 GN=RING1 PE... [more]
Q66J693.3e-2133.73E3 ubiquitin-protein ligase RING2-A OS=Xenopus laevis OX=8355 GN=rnf2-a PE=2 SV=... [more]
Q7ZWM84.3e-2133.90E3 ubiquitin-protein ligase RING2-B OS=Xenopus laevis OX=8355 GN=rnf2-b PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_038881714.11.5e-236100.00putative E3 ubiquitin-protein ligase RING1a isoform X1 [Benincasa hispida][more]
XP_004134950.15.7e-20488.32putative E3 ubiquitin-protein ligase RING1a [Cucumis sativus] >KGN49242.1 hypoth... [more]
KAA0052674.11.7e-20087.38putative E3 ubiquitin-protein ligase RING1a [Cucumis melo var. makuwa][more]
XP_008439830.15.0e-20087.15PREDICTED: putative E3 ubiquitin-protein ligase RING1a [Cucumis melo][more]
XP_023543674.13.3e-19683.61putative E3 ubiquitin-protein ligase RING1a isoform X2 [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
A0A0A0KLI22.7e-20488.32RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517990 P... [more]
A0A5A7UBC48.3e-20187.38Putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3AZN32.4e-20087.15putative E3 ubiquitin-protein ligase RING1a OS=Cucumis melo OX=3656 GN=LOC103484... [more]
A0A6J1ECT64.0e-19583.37putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1INR97.0e-19282.90putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita maxima OX=36... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 63..102
e-value: 1.0E-6
score: 38.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 63..103
score: 12.1057
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 18..133
e-value: 3.2E-18
score: 67.5
NoneNo IPR availablePFAMPF13923zf-C3HC4_2coord: 62..102
e-value: 3.5E-12
score: 45.9
NoneNo IPR availableGENE3D3.10.20.90coord: 296..381
e-value: 1.2E-5
score: 27.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..241
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 159..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..45
NoneNo IPR availablePANTHERPTHR46537:SF3OS11G0578200 PROTEINcoord: 1..424
NoneNo IPR availableCDDcd16531RING-HC_RING1_likecoord: 63..103
e-value: 1.54387E-23
score: 90.5691
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 53..134
IPR044592Putative E3 ubiquitin-protein ligase RING1a/bPANTHERPTHR46537OS11G0578200 PROTEINcoord: 1..424
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 79..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000302Bhi03M000302mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0046872 metal ion binding