Bhi03G000149 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000149
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Hydrolase_4 domain-containing protein
Location	chr3: 4059492 .. 4060469 (-)
RNA-Seq Expression	Bhi03G000149
Synteny	Bhi03G000149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCATCCAATTAACCAGGCCAACGAGAACAGCCCCTATGGAGGGTTAACAAGGGAAGAATTCTACAAGAAACACAAAGTTACTCACCATGAAAGCTTCATCCTCAATGCCCAGAAGATGAAAATCTTCACTCAATCATGGCGCCCTGATTCTGACTCAGAGCTCAAAGGGGTTGTAGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATATTCGAGCTCACTGCCGTCGCCATAGCTAAACTTGGGTTCCTCGTTTGCTCGCTGGACCTTCAAGGCCATGGCCGCTCCGAGGGAGCCCCTGGTTCGATCCCAGATATCGAACTGCTTGTTCTCGATTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTAACCTCCCTGCTTTTCTCTACGGGGAATCCCTCGGCGGTGCAATTTCGATCCTGATTTCCTTGAAGCAAAAGGGGGTTTGGAACGGAATCGTGTTGAACGGTTCCATGTGCGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCACCGTCATTGCGATTGGTGATAACGAAGCCCGTCGCGAGCAAATCGTACAAAGAAGAGTGGAAGAGGAGATTGGTGGCGAGAAACCCTAATCGGCGATTTTCAGGAAAACCGCCGATGGCGACAGCATTGGAGTTTCTCCGAGTGTGCGAGTACATAAAGAGAAACTGCCATGAAATTAGGGTCCCAATGCTGATGGTTCATGGAGAAGACGACGTGGTTTGCGATAGTTGGTCGGCGAGATTTGTGTTCGAATCGGCGGAGAGTGAGGACAAGACGTTGAAGGTGTATCCGGGAATGTGGCACCAGTTGATCGGAGAGCCGAAGGAAGATGTGGAGGTTGTTTATGGCACCATTTTCACCTGGTTAGTTGATAGGGCAGAGAAGACTAACAACAACAAGATCATCAATACGTAA

mRNA sequence

ATGTTGCATCCAATTAACCAGGCCAACGAGAACAGCCCCTATGGAGGGTTAACAAGGGAAGAATTCTACAAGAAACACAAAGTTACTCACCATGAAAGCTTCATCCTCAATGCCCAGAAGATGAAAATCTTCACTCAATCATGGCGCCCTGATTCTGACTCAGAGCTCAAAGGGGTTGTAGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATATTCGAGCTCACTGCCGTCGCCATAGCTAAACTTGGGTTCCTCGTTTGCTCGCTGGACCTTCAAGGCCATGGCCGCTCCGAGGGAGCCCCTGGTTCGATCCCAGATATCGAACTGCTTGTTCTCGATTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTAACCTCCCTGCTTTTCTCTACGGGGAATCCCTCGGCGGTGCAATTTCGATCCTGATTTCCTTGAAGCAAAAGGGGGTTTGGAACGGAATCGTGTTGAACGGTTCCATGTGCGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCACCGTCATTGCGATTGGTGATAACGAAGCCCGTCGCGAGCAAATCGTACAAAGAAGAGTGGAAGAGGAGATTGGTGGCGAGAAACCCTAATCGGCGATTTTCAGGAAAACCGCCGATGGCGACAGCATTGGAGTTTCTCCGAGTGTGCGAGTACATAAAGAGAAACTGCCATGAAATTAGGGTCCCAATGCTGATGGTTCATGGAGAAGACGACGTGGTTTGCGATAGTTGGTCGGCGAGATTTGTGTTCGAATCGGCGGAGAGTGAGGACAAGACGTTGAAGGTGTATCCGGGAATGTGGCACCAGTTGATCGGAGAGCCGAAGGAAGATGTGGAGGTTGTTTATGGCACCATTTTCACCTGGTTAGTTGATAGGGCAGAGAAGACTAACAACAACAAGATCATCAATACGTAA

Coding sequence (CDS)

ATGTTGCATCCAATTAACCAGGCCAACGAGAACAGCCCCTATGGAGGGTTAACAAGGGAAGAATTCTACAAGAAACACAAAGTTACTCACCATGAAAGCTTCATCCTCAATGCCCAGAAGATGAAAATCTTCACTCAATCATGGCGCCCTGATTCTGACTCAGAGCTCAAAGGGGTTGTAGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATATTCGAGCTCACTGCCGTCGCCATAGCTAAACTTGGGTTCCTCGTTTGCTCGCTGGACCTTCAAGGCCATGGCCGCTCCGAGGGAGCCCCTGGTTCGATCCCAGATATCGAACTGCTTGTTCTCGATTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTAACCTCCCTGCTTTTCTCTACGGGGAATCCCTCGGCGGTGCAATTTCGATCCTGATTTCCTTGAAGCAAAAGGGGGTTTGGAACGGAATCGTGTTGAACGGTTCCATGTGCGGAATCTCGGCGAAATTCAAACCGATTTGGCCATTGGAGAAGCTTCTTCCAATAGCAGCGTCTTTAGCACCGTCATTGCGATTGGTGATAACGAAGCCCGTCGCGAGCAAATCGTACAAAGAAGAGTGGAAGAGGAGATTGGTGGCGAGAAACCCTAATCGGCGATTTTCAGGAAAACCGCCGATGGCGACAGCATTGGAGTTTCTCCGAGTGTGCGAGTACATAAAGAGAAACTGCCATGAAATTAGGGTCCCAATGCTGATGGTTCATGGAGAAGACGACGTGGTTTGCGATAGTTGGTCGGCGAGATTTGTGTTCGAATCGGCGGAGAGTGAGGACAAGACGTTGAAGGTGTATCCGGGAATGTGGCACCAGTTGATCGGAGAGCCGAAGGAAGATGTGGAGGTTGTTTATGGCACCATTTTCACCTGGTTAGTTGATAGGGCAGAGAAGACTAACAACAACAAGATCATCAATACGTAA

Protein sequence

MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVEVVYGTIFTWLVDRAEKTNNNKIINT

Homology

[Close]

BLAST of Bhi03G000149 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.3e-94
Identity = 159/317 (50.16%), Postives = 225/317 (70.98%), Query Frame = 0


Query: 2   LHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVVA 61
           LHP+++AN +SP+G L+  EFY +H VTH  +++ N   +K+FTQ W P +   L G++A
Sbjct: 3   LHPVSEANASSPFGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIA 62

Query: 62  MVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFD 121
           +VHG+T +S W  +LT+V  AK G+L C++D QGHG S+G    IP+I L+V DC  FFD
Sbjct: 63  VVHGFTGESSWFLQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFD 122

Query: 122 SVREQNPN--LPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEK 181
             R+++ +  LPAFLY ESLGGAI++ I+L+QK  WNG++L+G+MC IS KFKP WPL+ 
Sbjct: 123 DFRKRHASSFLPAFLYSESLGGAIALYITLRQKHQWNGLILSGAMCSISHKFKPPWPLQH 182

Query: 182 LLPIAASLAPSLRLVITK-PVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCE 241
           LL +AA+L P+ R+V T+  +A  S+KE WKR+L   NPNR   GKP  ATA E +RVCE
Sbjct: 183 LLTLAATLIPTWRVVPTRGSIAGVSFKEPWKRKLAYANPNRTV-GKPRAATAYELVRVCE 242

Query: 242 YIKRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKED 301
            ++    E+ VP+++VHG DDVVCD  S   ++    S DKT+K+YPGMWHQLIGE +E+
Sbjct: 243 DLQNRFEEVEVPLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLIGESEEN 302

Query: 302 VEVVYGTIFTWLVDRAE 316
           V++V+G +  W+  R+E
Sbjct: 303 VDLVFGDVLDWIKTRSE 317

[Close]

BLAST of Bhi03G000149 vs. TAIR 10
Match: AT5G19290.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.5e-93
Identity = 161/318 (50.63%), Postives = 223/318 (70.13%), Query Frame = 0


Query: 2   LHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVVA 61
           LHPI+ ANE++P+G LT +EFY KH V+H  +FI N + +K+FTQ W P   ++  G++A
Sbjct: 3   LHPISDANEHNPFGSLTADEFYAKHSVSHSSAFITNPRGLKLFTQWWSPLPPTKPIGIIA 62

Query: 62  MVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFD 121
           +VHG+T +S W  +LT++  AK GF+ C++D QGHG S+G    IPDI  +V DC  FFD
Sbjct: 63  VVHGFTGESSWFLQLTSILFAKSGFITCAIDHQGHGFSDGLIAHIPDINPVVDDCISFFD 122

Query: 122 SVR-EQNP-NLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEK 181
             R  Q P +LP FLY ESLGGAI++ ISL+Q+GVW+G++LNG+MCGIS KFKP WPLE 
Sbjct: 123 DFRSRQTPSDLPCFLYSESLGGAIALYISLRQRGVWDGLILNGAMCGISDKFKPPWPLEH 182

Query: 182 LLPIAASLAPSLRLVITK-PVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCE 241
           LL + A+L P+ R++ T+  +   S+KE WKR+L   +P RR   KP  ATA E +RVC+
Sbjct: 183 LLFVVANLIPTWRVIPTRGSIPDVSFKEPWKRKLAMASP-RRTVAKPRAATAYELIRVCK 242

Query: 242 YIKRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKED 301
            ++    E+ VP+L+VHG  DVVCD      +   A SEDKT+K+YP +WHQ+IGE +E 
Sbjct: 243 DLQGRFEEVEVPLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMIGESEEK 302

Query: 302 VEVVYGTIFTWLVDRAEK 317
           V++VYG + +WL  RAE+
Sbjct: 303 VDLVYGDMLSWLKSRAER 319

[Close]

BLAST of Bhi03G000149 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 9.4e-64
Identity = 118/304 (38.82%), Postives = 190/304 (62.50%), Query Frame = 0


Query: 14  YGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWI 73
           +G  + E+++    +   +SF  + + + +FT+SW P S S  +G++ MVHGY +D  W 
Sbjct: 13  WGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYGNDVSWT 72

Query: 74  FELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNP---NL 133
           F+ T + +A++GF   +LD++GHGRS+G    +P ++L+V D   FF+S++ QNP    L
Sbjct: 73  FQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIK-QNPKFQGL 132

Query: 134 PAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPS 193
           P FL+GES+GGAI +LI       ++G VL   MC IS K +P WP+++ L + +   P+
Sbjct: 133 PRFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPT 192

Query: 194 LRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVP 253
             +V T+ +  KS K E K+ +  RNP  R++ KP + T +E LRV +Y+ +   ++ +P
Sbjct: 193 WAIVPTEDLLEKSIKVEEKKPIAKRNP-MRYNEKPRLGTVMELLRVTDYLGKKLKDVSIP 252

Query: 254 MLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWH-QLIGEPKEDVEVVYGTIFTW 313
            ++VHG  D V D   +R ++E A+S+DKTLK+Y GM H  L GEP +++E+V   I +W
Sbjct: 253 FIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVRKDIVSW 312

[Close]

BLAST of Bhi03G000149 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.8e-51
Identity = 106/294 (36.05%), Postives = 167/294 (56.80%), Query Frame = 0


Query: 28  VTHHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFL 87
           V + E F+LN++ MK+FT  W+P    E K ++ + HGY  +S       A  +A  GF 
Sbjct: 2   VMYEEDFVLNSRGMKLFTCVWKP-VKQEPKALLFLCHGYAMESSITMNSAATRLANAGFA 61

Query: 88  VCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSV--REQNPNLPAFLYGESLGGAISI 147
           V  +D +GHG+SEG  G I + + LV D +  + ++  RE+N     FL GES+GGA+ +
Sbjct: 62  VYGMDYEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVL 121

Query: 148 LISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYK 207
           L++ K+   W+G VL   MC ++ + KP   +  +L   A   P+ ++V    +   + K
Sbjct: 122 LLARKKPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIK 181

Query: 208 EEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 267
           E   R  V  N    + G+P + TA + L V   +++N H++ +P +++HGEDD V D  
Sbjct: 182 EPHIRNQVREN-KYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKS 241

Query: 268 SARFVFESAESEDKTLKVYPGMWHQLI-GEPKEDVEVVYGTIFTWLVDRAEKTN 319
            ++ ++E A S DKT K+YP MWH L+ GE  E+ E+V+G I  WL DRA  +N
Sbjct: 242 ISKMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRATDSN 293

[Close]

BLAST of Bhi03G000149 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 198.7 bits (504), Expect = 7.0e-51
Identity = 103/286 (36.01%), Postives = 165/286 (57.69%), Query Frame = 0


Query: 28  VTHHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFL 87
           + + ESFI N + MK+FT  W P +  E K +V + HGY  +       TA  + K GF 
Sbjct: 8   IKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKAGFA 67

Query: 88  VCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSV--REQNPNLPAFLYGESLGGAISI 147
           V  +D +GHG+S+G    +P+ + LV D +  + S+  +E+N     FL GES+GGA+ +
Sbjct: 68  VYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVLL 127

Query: 148 LISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYK 207
           L+  K+   W+G VL   MC I+ + KP   +  +L   + + PS +++  + +   ++K
Sbjct: 128 LLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAFK 187

Query: 208 EEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 267
           +   R+ V  NP   + G+P + TA E LRV   +++  +E+ +P +++HGEDD V D  
Sbjct: 188 QPEIRKQVRENP-YCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDKA 247

Query: 268 SARFVFESAESEDKTLKVYPGMWHQLI-GEPKEDVEVVYGTIFTWL 311
            +R ++E A S DKT K+YPGMWH L+ GE  E++E V+  I  WL
Sbjct: 248 VSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWL 291

[Close]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.8e-47
Identity = 104/307 (33.88%), Postives = 172/307 (56.03%), Query Frame = 0


Query: 14  YGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWI 73
           +G +  EE+Y    V + +S+       K+FTQS+ P  D E+KG V M HGY SD+ W+
Sbjct: 23  WGTMPEEEYYTSQGVRNSKSY-FETPNGKLFTQSFLP-LDGEIKGTVYMSHGYGSDTSWM 82

Query: 74  FELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNP--NLP 133
           F+   ++ +  G+ V + DL GHGRS+G    + D+E +      FF  VR  +P  +LP
Sbjct: 83  FQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLP 142

Query: 134 AFLYGESLGGAISILISLK-QKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPS 193
           AFL+GES+GG +++L+  + +   W G++ +  +  I    KP         +   LA +
Sbjct: 143 AFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLADT 202

Query: 194 LRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVP 253
              +    +  K+ K+  K +++A NP +R++GKP + T  E LR  +Y++ N  ++ +P
Sbjct: 203 WAAMPDNKMVGKAIKDPEKLKIIASNP-QRYTGKPRVGTMRELLRKTQYVQENFGKVTIP 262

Query: 254 MLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLI-GEPKEDVEVVYGTIFTW 313
           +   HG  D V    S++ ++E A S DKTLK+Y GM+H LI GEP E+ E+V   +  W
Sbjct: 263 VFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREW 322

Query: 314 LVDRAEK 317
           + ++ +K
Sbjct: 323 IDEKVKK 326

[Close]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 84/288 (29.17%), Postives = 133/288 (46.18%), Query Frame = 0


Query: 35  ILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG    SG   EL  + +  L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPTGTP--KALIFVSHGAGEHSGRYEELARMLMG-LDLLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNPNLPAFLYGESLGGAISILISLKQKGV 154
           GHG+SEG    + D  + V D     DS+++  P LP FL G S+GGAI+IL + ++ G 
Sbjct: 82  GHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGH 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYKEEWKRRLVA 214
           + G+VL             I PL    P +A+    L   +   V            +++
Sbjct: 142 FAGMVL-------------ISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN      ++  P +  A       ++ L     ++R   ++ VP L++ G  D +CDS 
Sbjct: 202 RNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSK 261

Query: 275 SARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVEVVYGTIFTWLVDR 314
            A  + E A+S+DKTLK+Y G +H L  E  E    V+  I  W+  R
Sbjct: 262 GAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQR 293

[Close]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.4e-24
Identity = 76/290 (26.21%), Postives = 126/290 (43.45%), Query Frame = 0


Query: 29  THHESFILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFLV 88
           T  E        ++I    W PD+D   +GVV + HGY   +G  +   A      G LV
Sbjct: 5   TRSEHSFAGVGGVRIVYDVWTPDTDP--RGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLV 64

Query: 89  CSLDLQGHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNPNLPAFLYGESLGGAISILIS 148
            +LD +GHGRS G    + D+   V D           +P LP  + G S+GG I     
Sbjct: 65  YALDHRGHGRSGGKRVHLRDLSEFVEDFRTLVGIAANDHPTLPRIVLGHSMGGGIVFAYG 124

Query: 149 LKQKGVWNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRL-------VITKPVAS 208
            +  G ++ +VL+G          P+  L  +  +   LAP + +       V   P   
Sbjct: 125 ARYPGEYSAMVLSGPAVNAHDGVSPV--LVAVAKVLGKLAPGIPVENLDADAVSRDPEVV 184

Query: 209 KSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYIKRNCHEIRVPMLMVHGEDDVV 268
            +YK +               GK P   A   + + + + +    +  P+L+VHG+ D +
Sbjct: 185 AAYKAD----------PMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRL 244

Query: 269 CDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVEVVYGTIFTWLV 312
                +R + +   SED  LKVYPG++H++  EP++  ++V   + +W+V
Sbjct: 245 IPVAGSRLLVDRVASEDVHLKVYPGLYHEVFNEPEQ--KLVLDDVTSWIV 277

[Close]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.1e-24
Identity = 81/288 (28.12%), Postives = 135/288 (46.88%), Query Frame = 0


Query: 35  ILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL A  +  L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPSGTP--KALIFVSHGAGEHCGRYDEL-AHMLKGLDMLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNPNLPAFLYGESLGGAISILISLKQKGV 154
           GHG+SEG    + D ++ V D     D++++  P++P FL G S+GGAISIL++ ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISILVAAERPTY 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYKEEWKRRLVA 214
           ++G+VL             I PL    P +AS    L   +   V            +++
Sbjct: 142 FSGMVL-------------ISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN +    ++  P +  A       ++ L     ++R    + +P L++ G  D +CDS 
Sbjct: 202 RNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSK 261

Query: 275 SARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVEVVYGTIFTWLVDR 314
            A  + ES+ S+DKTLK+Y G +H L  E  E    V   + +W+  R
Sbjct: 262 GAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHR 293

[Close]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.1e-23
Identity = 82/288 (28.47%), Postives = 134/288 (46.53%), Query Frame = 0


Query: 35  ILNAQKMKIFTQSWRPDSDSELKGVVAMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG     G   EL A  + +L  LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPSGTP--KALIFVSHGAGEHCGRYDEL-AQMLKRLDMLVFAHDHV 81

Query: 95  GHGRSEGAPGSIPDIELLVLDCTLFFDSVREQNPNLPAFLYGESLGGAISILISLKQKGV 154
           GHG+SEG    + D ++ V D     ++V++  P +P FL G S+GGAISIL + ++   
Sbjct: 82  GHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTH 141

Query: 155 WNGIVLNGSMCGISAKFKPIWPLEKLLPIAASLAPSLRLVITKPVASKSYKEEWKRRLVA 214
           ++G++L             I PL    P +AS    L   +   V            +++
Sbjct: 142 FSGMIL-------------ISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLS 201

Query: 215 RNPNR--RFSGKPPMATA-------LEFLRVCEYIKRNCHEIRVPMLMVHGEDDVVCDSW 274
           RN +    ++  P +  A       ++ L     ++R    + +P L++ G  D +CDS 
Sbjct: 202 RNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSK 261

Query: 275 SARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVEVVYGTIFTWLVDR 314
            A  + ES+ S+DKTLK+Y G +H L  E  E    V   I TW+  R
Sbjct: 262 GAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHR 293

[Close]

BLAST of Bhi03G000149 vs. NCBI nr
Match: XP_038881607.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.0e-189
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV
Sbjct: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNNKIINT 326
           VVYGTIFTWLVDRAEKTNNNKIINT
Sbjct: 301 VVYGTIFTWLVDRAEKTNNNKIINT 325

[Close]

BLAST of Bhi03G000149 vs. NCBI nr
Match: KAA0052486.1 (caffeoylshikimate esterase-like [Cucumis melo var. makuwa] >TYK13337.1 caffeoylshikimate esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 623.6 bits (1607), Expect = 9.4e-175
Identity = 297/320 (92.81%), Postives = 309/320 (96.56%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSWRPDSD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT VAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNGSMCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSAR+VFE+AESEDKTLKVYPGMWHQLIGE KEDVE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNN 321
           +VYGTI  WLVD+A+K NN+
Sbjct: 301 IVYGTILNWLVDKADKANNS 320

[Close]

BLAST of Bhi03G000149 vs. NCBI nr
Match: XP_008441075.1 (PREDICTED: caffeoylshikimate esterase-like [Cucumis melo])

HSP 1 Score: 621.3 bits (1601), Expect = 4.7e-174
Identity = 296/320 (92.50%), Postives = 308/320 (96.25%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSWRPDSD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT VAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNG MCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSAR+VFE+AESEDKTLKVYPGMWHQLIGE KEDVE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNN 321
           +VYGTI  WLVD+A+K NN+
Sbjct: 301 IVYGTILNWLVDKADKANNS 320

[Close]

BLAST of Bhi03G000149 vs. NCBI nr
Match: KGN49464.1 (hypothetical protein Csa_003642 [Cucumis sativus])

HSP 1 Score: 617.8 bits (1592), Expect = 5.2e-173
Identity = 295/319 (92.48%), Postives = 309/319 (96.87%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPI+QANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSW+PDSDS+LKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT +AIAKLGFLVCSLDLQGHGRSEGAPGSI DIELLVLDCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVP+LMVHGEDDVVCDSWSAR+V+E+AESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 VVYGTIFTWLVDRAEKTNN 320
           VVYGTIF WLVDRAEK +N
Sbjct: 301 VVYGTIFNWLVDRAEKADN 319

[Close]

BLAST of Bhi03G000149 vs. NCBI nr
Match: KAG6594422.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 604.7 bits (1558), Expect = 4.5e-169
Identity = 288/321 (89.72%), Postives = 305/321 (95.02%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPIN+ANENSPYGGLTREEFYKKHKVTH+ESFILNAQKMKIFTQSWRP +D+ELKGVV
Sbjct: 1   MSHPINEANENSPYGGLTREEFYKKHKVTHYESFILNAQKMKIFTQSWRPAADAELKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGY+SDSGWIFELTAVAIAK GFLVCSLDLQGHGRSEG+PG IPDI+LL+LDCTLFF
Sbjct: 61  AMVHGYSSDSGWIFELTAVAIAKYGFLVCSLDLQGHGRSEGSPGYIPDIQLLLLDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNP LPAFLYGESLGGAIS+LI+LKQ  VWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISMLIALKQMEVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LP+AASLAPSLRLV++KP+A KSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPLAASLAPSLRLVVSKPLAGKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           K NCHEIR+PMLMVHGEDDVVCDS SARFVFESA SEDKTLKVYPG WHQLIGEPKE+VE
Sbjct: 241 KTNCHEIRIPMLMVHGEDDVVCDSRSARFVFESAASEDKTLKVYPGTWHQLIGEPKENVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNNK 322
           +VYGTIF WLVDRAEK  N K
Sbjct: 301 LVYGTIFNWLVDRAEKAKNKK 321

[Close]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL
Match: A0A5A7UE91 (Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009620 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.6e-175
Identity = 297/320 (92.81%), Postives = 309/320 (96.56%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSWRPDSD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT VAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNGSMCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSAR+VFE+AESEDKTLKVYPGMWHQLIGE KEDVE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNN 321
           +VYGTI  WLVD+A+K NN+
Sbjct: 301 IVYGTILNWLVDKADKANNS 320

[Close]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL
Match: A0A1S3B390 (caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 296/320 (92.50%), Postives = 308/320 (96.25%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSWRPDSD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT VAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNG MCGIS KFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVPMLMVHGEDDVVCDSWSAR+VFE+AESEDKTLKVYPGMWHQLIGE KEDVE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 VVYGTIFTWLVDRAEKTNNN 321
           +VYGTI  WLVD+A+K NN+
Sbjct: 301 IVYGTILNWLVDKADKANNS 320

[Close]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL
Match: A0A0A0KKV9 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500 PE=4 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.5e-173
Identity = 295/319 (92.48%), Postives = 309/319 (96.87%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPI+QANENSPYG LTREEFYKKH VTHHESFILNAQKMKIFTQSW+PDSDS+LKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGWIFELT +AIAKLGFLVCSLDLQGHGRSEGAPGSI DIELLVLDCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DS+REQ+PNLPAFLYGESLGGAISILISLKQ+GVWNGIVLNGSMCGISAKFKPIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLVI+KPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           KRNCHEIRVP+LMVHGEDDVVCDSWSAR+V+E+AESEDKTLKVYPGMWHQLIGE KE+VE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 VVYGTIFTWLVDRAEKTNN 320
           VVYGTIF WLVDRAEK +N
Sbjct: 301 VVYGTIFNWLVDRAEKADN 319

[Close]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL
Match: A0A6J1ECA8 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE=4 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 3.7e-169
Identity = 294/326 (90.18%), Postives = 305/326 (93.56%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYGGLTREEFYKKH VTHHE+FILNAQKMKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNP LPAFLYGESLGGAISILI LKQ+GVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV++KP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SARFVFESA SEDKTLKVYPGMWHQLIGE  E+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 VVYGTIFTWLVDRAEKTN-NNKIINT 326
           +VY TIF WLVDRAEK    NK  NT
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNT 326

[Close]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL
Match: A0A6J1IPF3 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 288/319 (90.28%), Postives = 301/319 (94.36%), Query Frame = 0


Query: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60
           M HPINQANENSPYGGLTRE+FY+KH VTH E+FILNAQKMKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWIFELTAVA+AK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180
           DSVREQNP LPAFLYGESLGGAISILI LKQ+GVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPSLRLV +KP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SARFVFESA SEDKTLKVYPGMWHQLIGE  E+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 VVYGTIFTWLVDRAEKTNN 320
           +VY TIF WLVDRAEK  N
Sbjct: 301 IVYATIFNWLVDRAEKAKN 319

The following BLAST results are available for this feature:

BLAST of Bhi03G000149 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14980.1	1.3e-94	50.16	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19290.1	2.5e-93	50.63	alpha/beta-Hydrolases superfamily protein	[more]
AT1G11090.1	9.4e-64	38.82	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39400.1	1.8e-51	36.05	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	7.0e-51	36.01	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Bhi03G000149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	7.8e-47	33.88	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
Q99685	1.4e-24	29.17	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
A0QNZ7	5.4e-24	26.21	Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(...	[more]
O35678	7.1e-24	28.13	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q8R431	2.1e-23	28.47	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]

BLAST of Bhi03G000149 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881607.1	3.0e-189	100.00	caffeoylshikimate esterase-like [Benincasa hispida]	[more]
KAA0052486.1	9.4e-175	92.81	caffeoylshikimate esterase-like [Cucumis melo var. makuwa] >TYK13337.1 caffeoyls...	[more]
XP_008441075.1	4.7e-174	92.50	PREDICTED: caffeoylshikimate esterase-like [Cucumis melo]	[more]
KGN49464.1	5.2e-173	92.48	hypothetical protein Csa_003642 [Cucumis sativus]	[more]
KAG6594422.1	4.5e-169	89.72	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Bhi03G000149 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UE91	4.6e-175	92.81	Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3B390	2.3e-174	92.50	caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=...	[more]
A0A0A0KKV9	2.5e-173	92.48	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500...	[more]
A0A6J1ECA8	3.7e-169	90.18	caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE...	[more]
A0A6J1IPF3	1.6e-167	90.28	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 134..147 score: 40.64 coord: 85..100 score: 35.56 coord: 251..265 score: 29.43
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 55..295 e-value: 3.6E-55 score: 186.8
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 19..321 e-value: 1.3E-51 score: 177.6
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 40..313
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 2..319
None	No IPR available	PANTHER	PTHR11614:SF101	CAFFEOYLSHIKIMATE ESTERASE-LIKE	coord: 2..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000149	Bhi03M000149	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity