Bhi03G000121 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000121
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Chaperone protein ClpB
Location	chr3: 3234484 .. 3234750 (-)
RNA-Seq Expression	Bhi03G000121
Synteny	Bhi03G000121

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGATATGAGGAAGGAGGGCGCAACCGACAAAGGCAGTAAGGTGGAAGGAAGCCTTAAGTGTTGTTTTGTTTATGAAGTAAAGAGCTCACATCTCTGTCTTCAACACTCTTCTCGTGTTCTTCAAGTTTTAGATGATGGAAGATTGACCGATGGTCAAGGCCGTATTGTAGATTTCAGAAATACAGTCATTATCATGACTTCAAATCTTGGAGTTGAACATCTCCTTTCAAGGCTAATGGGCAAGTTGCTCGTGATCGAGTAA

mRNA sequence

ATGTTGGATATGAGGAAGGAGGGCGCAACCGACAAAGGCAGTAAGGTGGAAGGAAGCCTTAAGTGTTGTTTTGTTTATGAAGTAAAGAGCTCACATCTCTGTCTTCAACACTCTTCTCGTGTTCTTCAAGTTTTAGATGATGGAAGATTGACCGATGGTCAAGGCCGTATTGTAGATTTCAGAAATACAGTCATTATCATGACTTCAAATCTTGGAGTTGAACATCTCCTTTCAAGGCTAATGGGCAAGTTGCTCGTGATCGAGTAA

Coding sequence (CDS)

ATGTTGGATATGAGGAAGGAGGGCGCAACCGACAAAGGCAGTAAGGTGGAAGGAAGCCTTAAGTGTTGTTTTGTTTATGAAGTAAAGAGCTCACATCTCTGTCTTCAACACTCTTCTCGTGTTCTTCAAGTTTTAGATGATGGAAGATTGACCGATGGTCAAGGCCGTATTGTAGATTTCAGAAATACAGTCATTATCATGACTTCAAATCTTGGAGTTGAACATCTCCTTTCAAGGCTAATGGGCAAGTTGCTCGTGATCGAGTAA

Protein sequence

MLDMRKEGATDKGSKVEGSLKCCFVYEVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGKLLVIE

Homology

[Close]

BLAST of Bhi03G000121 vs. TAIR 10
Match: AT1G74310.1 (heat shock protein 101 )

HSP 1 Score: 77.4 bits (189), Expect = 6.3e-15
Identity = 39/58 (67.24%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGKL 85
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRN+VIIMTSNLG EHLL+ L GK+
Sbjct: 679 EVEKAHVAVFNT--LLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKV 734

[Close]

BLAST of Bhi03G000121 vs. TAIR 10
Match: AT2G25140.1 (casein lytic proteinase B4 )

HSP 1 Score: 57.4 bits (137), Expect = 6.8e-09
Identity = 25/40 (62.50%), Postives = 32/40 (80.00%), Query Frame = 0


Query: 41  VLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRL 81
           +LQ+LDDGR+TD QGR V F+N V+IMTSN+G  H+L  L
Sbjct: 775 LLQLLDDGRITDSQGRTVSFKNCVVIMTSNIGSHHILETL 814

[Close]

BLAST of Bhi03G000121 vs. TAIR 10
Match: AT5G15450.1 (casein lytic proteinase B3 )

HSP 1 Score: 55.1 bits (131), Expect = 3.4e-08
Identity = 25/37 (67.57%), Postives = 31/37 (83.78%), Query Frame = 0


Query: 42  LQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLS 79
           LQ+LDDGR+TD QGR V F NTVIIMTSN+G + +L+
Sbjct: 771 LQILDDGRVTDSQGRTVSFTNTVIIMTSNVGSQFILN 807

[Close]

BLAST of Bhi03G000121 vs. TAIR 10
Match: AT3G48870.1 (Clp ATPase )

HSP 1 Score: 53.1 bits (126), Expect = 1.3e-07
Identity = 22/32 (68.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 41  VLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLG 73
           +LQ+L+DGRLTD +GR VDF+NT++IMTSN+G
Sbjct: 751 MLQILEDGRLTDSKGRTVDFKNTLLIMTSNVG 782

[Close]

BLAST of Bhi03G000121 vs. TAIR 10
Match: AT3G48870.2 (Clp ATPase )

HSP 1 Score: 53.1 bits (126), Expect = 1.3e-07
Identity = 22/32 (68.75%), Postives = 30/32 (93.75%), Query Frame = 0


Query: 41  VLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLG 73
           +LQ+L+DGRLTD +GR VDF+NT++IMTSN+G
Sbjct: 720 MLQILEDGRLTDSKGRTVDFKNTLLIMTSNVG 751

[Close]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot
Match: Q6F2Y7 (Chaperone protein ClpB1 OS=Oryza sativa subsp. japonica OX=39947 GN=CLPB1 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 3.1e-14
Identity = 39/57 (68.42%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLL+ ++GK
Sbjct: 680 EVEKAHVAVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLAGMVGK 734

[Close]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot
Match: P42730 (Chaperone protein ClpB1 OS=Arabidopsis thaliana OX=3702 GN=CLPB1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 8.9e-14
Identity = 39/58 (67.24%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGKL 85
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRN+VIIMTSNLG EHLL+ L GK+
Sbjct: 679 EVEKAHVAVFNT--LLQVLDDGRLTDGQGRTVDFRNSVIIMTSNLGAEHLLAGLTGKV 734

[Close]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot
Match: O83110 (Chaperone protein ClpB OS=Treponema pallidum (strain Nichols) OX=243276 GN=clpB PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-11
Identity = 31/37 (83.78%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 42  LQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLS 79
           LQ+LDDGRLTDGQGR+VDFRNT+IIMTSN+G EH+LS
Sbjct: 695 LQILDDGRLTDGQGRVVDFRNTIIIMTSNIGSEHILS 731

[Close]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot
Match: P74459 (Chaperone protein ClpB 1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=clpB1 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.1e-11
Identity = 32/52 (61.54%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLS 79
           EV+ +HL +   + +LQVLDDGR+TD QGR+VDFRNT+I+MTSN+G +H+LS
Sbjct: 693 EVEKAHLDV--FNILLQVLDDGRITDSQGRVVDFRNTIIVMTSNIGSDHILS 742

[Close]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot
Match: Q7CEG6 (Chaperone protein ClpB OS=Brucella suis biovar 1 (strain 1330) OX=204722 GN=clpB PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 7.1e-11
Identity = 32/38 (84.21%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 41  VLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLS 79
           +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG E+L++
Sbjct: 690 LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEYLVN 727

[Close]

BLAST of Bhi03G000121 vs. NCBI nr
Match: XP_038881582.1 (chaperone protein ClpB-like [Benincasa hispida])

HSP 1 Score: 169.5 bits (428), Expect = 1.3e-38
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 4  MRKEGATDKGSKVEGSLKCCFVYEVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNT 63
          MRKEGATDKGSKVEGSLKCCFVYEVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNT
Sbjct: 1  MRKEGATDKGSKVEGSLKCCFVYEVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNT 60

Query: 64 VIIMTSNLGVEHLLSRLMGKLLVIE 89
          VIIMTSNLGVEHLLSRLMGKLLVI+
Sbjct: 61 VIIMTSNLGVEHLLSRLMGKLLVID 85

[Close]

BLAST of Bhi03G000121 vs. NCBI nr
Match: XP_012080363.1 (chaperone protein ClpB1 [Jatropha curcas] >KDP31333.1 hypothetical protein JCGZ_11709 [Jatropha curcas])

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-12
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. NCBI nr
Match: XP_021615851.1 (chaperone protein ClpB1 [Manihot esculenta] >OAY47529.1 hypothetical protein MANES_06G085600v8 [Manihot esculenta])

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-12
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. NCBI nr
Match: XP_031372983.1 (chaperone protein ClpB1 [Punica granatum] >OWM90377.1 hypothetical protein CDL15_Pgr014679 [Punica granatum] >PKI77942.1 hypothetical protein CRG98_001562 [Punica granatum])

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-12
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. NCBI nr
Match: XP_031258763.1 (chaperone protein ClpB1 [Pistacia vera] >XP_031275780.1 chaperone protein ClpB1 [Pistacia vera])

HSP 1 Score: 82.8 bits (203), Expect = 1.6e-12
Identity = 42/58 (72.41%), Postives = 50/58 (86.21%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGKL 85
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK+
Sbjct: 682 EVEKAHIAVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGKV 737

[Close]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL
Match: A0A218XZ87 (chaperone protein ClpB1 OS=Punica granatum OX=22663 GN=LOC116188022 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.0e-13
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL
Match: A0A067K8K0 (Clp R domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11709 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.0e-13
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL
Match: A0A2C9VQM0 (Clp R domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G085600 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.0e-13
Identity = 43/57 (75.44%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +HL + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHLSVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL
Match: A0A059A5G2 (Clp R domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K02521 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.8e-13
Identity = 42/57 (73.68%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHIAVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

[Close]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL
Match: A0A6J1AJB7 (chaperone protein ClpB1 OS=Herrania umbratica OX=108875 GN=LOC110418515 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.0e-12
Identity = 42/57 (73.68%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 27  EVKSSHLCLQHSSRVLQVLDDGRLTDGQGRIVDFRNTVIIMTSNLGVEHLLSRLMGK 84
           EV+ +H+ + ++  +LQVLDDGRLTDGQGR VDFRNTVIIMTSNLG EHLLS LMGK
Sbjct: 678 EVEKAHISVFNT--LLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLMGK 732

The following BLAST results are available for this feature:

BLAST of Bhi03G000121 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74310.1	6.3e-15	67.24	heat shock protein 101	[more]
AT2G25140.1	6.8e-09	62.50	casein lytic proteinase B4	[more]
AT5G15450.1	3.4e-08	67.57	casein lytic proteinase B3	[more]
AT3G48870.1	1.3e-07	68.75	Clp ATPase	[more]
AT3G48870.2	1.3e-07	68.75	Clp ATPase	[more]

BLAST of Bhi03G000121 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6F2Y7	3.1e-14	68.42	Chaperone protein ClpB1 OS=Oryza sativa subsp. japonica OX=39947 GN=CLPB1 PE=2 S...	[more]
P42730	8.9e-14	67.24	Chaperone protein ClpB1 OS=Arabidopsis thaliana OX=3702 GN=CLPB1 PE=1 SV=2	[more]
O83110	1.1e-11	83.78	Chaperone protein ClpB OS=Treponema pallidum (strain Nichols) OX=243276 GN=clpB ...	[more]
P74459	4.1e-11	61.54	Chaperone protein ClpB 1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111...	[more]
Q7CEG6	7.1e-11	84.21	Chaperone protein ClpB OS=Brucella suis biovar 1 (strain 1330) OX=204722 GN=clpB...	[more]

BLAST of Bhi03G000121 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881582.1	1.3e-38	98.82	chaperone protein ClpB-like [Benincasa hispida]	[more]
XP_012080363.1	1.2e-12	75.44	chaperone protein ClpB1 [Jatropha curcas] >KDP31333.1 hypothetical protein JCGZ_...	[more]
XP_021615851.1	1.2e-12	75.44	chaperone protein ClpB1 [Manihot esculenta] >OAY47529.1 hypothetical protein MAN...	[more]
XP_031372983.1	1.2e-12	75.44	chaperone protein ClpB1 [Punica granatum] >OWM90377.1 hypothetical protein CDL15...	[more]
XP_031258763.1	1.6e-12	72.41	chaperone protein ClpB1 [Pistacia vera] >XP_031275780.1 chaperone protein ClpB1 ...	[more]

BLAST of Bhi03G000121 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A218XZ87	6.0e-13	75.44	chaperone protein ClpB1 OS=Punica granatum OX=22663 GN=LOC116188022 PE=3 SV=1	[more]
A0A067K8K0	6.0e-13	75.44	Clp R domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_11709 PE=3 ...	[more]
A0A2C9VQM0	6.0e-13	75.44	Clp R domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G085600 ...	[more]
A0A059A5G2	7.8e-13	73.68	Clp R domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K02521...	[more]
A0A6J1AJB7	1.0e-12	73.68	chaperone protein ClpB1 OS=Herrania umbratica OX=108875 GN=LOC110418515 PE=4 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003959	ATPase, AAA-type, core	PFAM	PF07724	AAA_2	coord: 41..77 e-value: 1.8E-10 score: 41.2
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 21..84 e-value: 9.6E-12 score: 46.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 39..78
None	No IPR available	PANTHER	PTHR11638:SF146	HEAT SHOCK PROTEIN 104	coord: 41..78
None	No IPR available	PANTHER	PTHR11638	ATP-DEPENDENT CLP PROTEASE	coord: 41..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000121	Bhi03M000121	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034605	cellular response to heat
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity