Bhi03G000083 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000083
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	9-cis-epoxycarotenoid dioxygenase
Location	chr3: 2257210 .. 2259286 (+)
RNA-Seq Expression	Bhi03G000083
Synteny	Bhi03G000083

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTTCTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTATATTTTATATTTTATATTTTATTCTTTTCTCTATTTCTAATTCTCCTTAAATTTTGCCTTCTCCTACTACTTCTTCTACTCACTCACTCACTCACTCTATCTTTTTAAAGCCATGATGGCGGCTTCTCCACCTCCTTCTTCTTCTTCTCTACTCGATCATTTGAGCTTTTCTAATTCCACCAGATCAAATTTCCCCGCTTTCAATTACAACAATAACGTCAAATTCATTAACCAAAACTCCACCCATAATCCCATTATTCACTCTGCTCTTCACTCTCCATCCGTTCTTCATTTCCCAAACCAAACTCCTAATTTCCACAACAATCTCACTCTCAAAGACCAAACCAACAAATCCCCTTTTTCCAATTGGAATCTCCTTCAAAAAACAGCTTCCATGGCTTTATACATGGTCGAATCCGCTTTAGTTTCTCACGAGCTTCAACACCCACTCCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAATTTCGCTCCCGTCCCCGAACAACCCGTTAAACATAACCTCCCTGTCACCGGAACCATCCCCGATTGTATTCACGGTGTTTATCTCCGTAACGGCGCTAATCCTCTCTTTCAACCAACTGCCGGACACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCACTATCAATGGCGGTTCCGCTAGCTACGCCTGCCGCTTCACTCAGACCCAACGGCTCGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCGATTGGGGAACTCCACGGGCACTCCGGAATCGCTCGCTTGTTACTGTTCTACGCGAGAGGAGTGTTTGGGTTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCAATAAACAGCTTCATTCCACAATGATCGCACATCCGAAAGTGGACCCAATCTCCAAAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAACCGTATCTGAAATACTTCCGATTCTCCCCCGACGGAATGAAATCCAAAGACGTCGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTAGTGATCCCCGATCAGCAAGTCGTGTTTAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAAGAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACGGATTCGAAGGATTTGATTTGGGTGGAATCGCCGGATACGTTCTGTTTTCATCTGTGGAATGCTTGGGAGGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATTTTCAACGAGTGCGAGGAGAATTTGAAAAGCGTTTTATCAGAAATTCGGCTGAATTTACGGACGGGGAAATCGACACGGCGGGCGATTATCAAGGAAGAGAAAGAACAGATGAATCTAGAAGCGGGAATGGTGAATAGAAACCGACTGGGGCGGAAAACCCGGTTTGCATATTTGGCGATTGCAGAGCCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTGGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAAAGGAAGAAGATGAAGGATACATTCTGGCGTTTGTTCATGATGAAAGGACATGGAAATCAGAGGTTCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATAAAATTACCTTCGAGAGTTCCATATGGATTTCATGGGACATTCGTTCAGTCAAACAGTTTGCAAAATCAAGCTTAATGCATTCAAGAAGAAGAGAAAAAAAAGGAGGAGAAATTACCAGAGGGATGGCTTAGAGACAGGTCCCCGGATTCTCTCCTATTAGCATTAAAAACCGAACCAACTTTTTGTTTGTGTTTTCT

mRNA sequence

TTTTTTTCTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTATATTTTATATTTTATATTTTATTCTTTTCTCTATTTCTAATTCTCCTTAAATTTTGCCTTCTCCTACTACTTCTTCTACTCACTCACTCACTCACTCTATCTTTTTAAAGCCATGATGGCGGCTTCTCCACCTCCTTCTTCTTCTTCTCTACTCGATCATTTGAGCTTTTCTAATTCCACCAGATCAAATTTCCCCGCTTTCAATTACAACAATAACGTCAAATTCATTAACCAAAACTCCACCCATAATCCCATTATTCACTCTGCTCTTCACTCTCCATCCGTTCTTCATTTCCCAAACCAAACTCCTAATTTCCACAACAATCTCACTCTCAAAGACCAAACCAACAAATCCCCTTTTTCCAATTGGAATCTCCTTCAAAAAACAGCTTCCATGGCTTTATACATGGTCGAATCCGCTTTAGTTTCTCACGAGCTTCAACACCCACTCCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAATTTCGCTCCCGTCCCCGAACAACCCGTTAAACATAACCTCCCTGTCACCGGAACCATCCCCGATTGTATTCACGGTGTTTATCTCCGTAACGGCGCTAATCCTCTCTTTCAACCAACTGCCGGACACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCACTATCAATGGCGGTTCCGCTAGCTACGCCTGCCGCTTCACTCAGACCCAACGGCTCGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCGATTGGGGAACTCCACGGGCACTCCGGAATCGCTCGCTTGTTACTGTTCTACGCGAGAGGAGTGTTTGGGTTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCAATAAACAGCTTCATTCCACAATGATCGCACATCCGAAAGTGGACCCAATCTCCAAAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAACCGTATCTGAAATACTTCCGATTCTCCCCCGACGGAATGAAATCCAAAGACGTCGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTAGTGATCCCCGATCAGCAAGTCGTGTTTAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAAGAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACGGATTCGAAGGATTTGATTTGGGTGGAATCGCCGGATACGTTCTGTTTTCATCTGTGGAATGCTTGGGAGGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATTTTCAACGAGTGCGAGGAGAATTTGAAAAGCGTTTTATCAGAAATTCGGCTGAATTTACGGACGGGGAAATCGACACGGCGGGCGATTATCAAGGAAGAGAAAGAACAGATGAATCTAGAAGCGGGAATGGTGAATAGAAACCGACTGGGGCGGAAAACCCGGTTTGCATATTTGGCGATTGCAGAGCCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTGGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAAAGGAAGAAGATGAAGGATACATTCTGGCGTTTGTTCATGATGAAAGGACATGGAAATCAGAGGTTCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATAAAATTACCTTCGAGAGTTCCATATGGATTTCATGGGACATTCGTTCAGTCAAACAGTTTGCAAAATCAAGCTTAATGCATTCAAGAAGAAGAGAAAAAAAAGGAGGAGAAATTACCAGAGGGATGGCTTAGAGACAGGTCCCCGGATTCTCTCCTATTAGCATTAAAAACCGAACCAACTTTTTGTTTGTGTTTTCT

Coding sequence (CDS)

ATGATGGCGGCTTCTCCACCTCCTTCTTCTTCTTCTCTACTCGATCATTTGAGCTTTTCTAATTCCACCAGATCAAATTTCCCCGCTTTCAATTACAACAATAACGTCAAATTCATTAACCAAAACTCCACCCATAATCCCATTATTCACTCTGCTCTTCACTCTCCATCCGTTCTTCATTTCCCAAACCAAACTCCTAATTTCCACAACAATCTCACTCTCAAAGACCAAACCAACAAATCCCCTTTTTCCAATTGGAATCTCCTTCAAAAAACAGCTTCCATGGCTTTATACATGGTCGAATCCGCTTTAGTTTCTCACGAGCTTCAACACCCACTCCCTAAAACCGCCGACCCACGTGTTCAAATCTCCGGCAATTTCGCTCCCGTCCCCGAACAACCCGTTAAACATAACCTCCCTGTCACCGGAACCATCCCCGATTGTATTCACGGTGTTTATCTCCGTAACGGCGCTAATCCTCTCTTTCAACCAACTGCCGGACACCATCTCTTCGACGGCGATGGAATGGTTCACGCTGTCACTATCAATGGCGGTTCCGCTAGCTACGCCTGCCGCTTCACTCAGACCCAACGGCTCGTTCAGGAACGGCGATTGGGGAGACCGGTTTTTCCTAAAGCGATTGGGGAACTCCACGGGCACTCCGGAATCGCTCGCTTGTTACTGTTCTACGCGAGAGGAGTGTTTGGGTTAGTGGACCATAAGAAAGGAACCGGAGTGGCGAACGCCGGTCTGGTGTATTTCAATGACCGGCTTTTGGCGATGTCAGAAGACGATTTGCCGTACCACGTGCGTATCACGCCTTCAGGTGATCTACAAACCGTAGGCCGTTACGATTTCAATAAACAGCTTCATTCCACAATGATCGCACATCCGAAAGTGGACCCAATCTCCAAAGAACTCTACGCCTTAAGCTACGACGTCGTTCGTAAACCGTATCTGAAATACTTCCGATTCTCCCCCGACGGAATGAAATCCAAAGACGTCGAAATCCCACTTGAAACTCCCACAATGATGCACGATTTCGCCATCACAGAGAATTTCGTAGTGATCCCCGATCAGCAAGTCGTGTTTAAGCTTCAAGAAATGGTAAAAGGCGGATCTCCGGTGATTTACGACAAGAAGAAGAAATCCCGATTCGGAATTCTCCCGAAAAATGCGACGGATTCGAAGGATTTGATTTGGGTGGAATCGCCGGATACGTTCTGTTTTCATCTGTGGAATGCTTGGGAGGAGCCGGAGACGGAGGAGGTGGTGGTGATTGGGTCGTGTATGACGCCGCCGGATTCGATTTTCAACGAGTGCGAGGAGAATTTGAAAAGCGTTTTATCAGAAATTCGGCTGAATTTACGGACGGGGAAATCGACACGGCGGGCGATTATCAAGGAAGAGAAAGAACAGATGAATCTAGAAGCGGGAATGGTGAATAGAAACCGACTGGGGCGGAAAACCCGGTTTGCATATTTGGCGATTGCAGAGCCATGGCCGAAGGTATCGGGATTTGCGAAGGTTGATCTGGTAACAGGGGAAGTAAAAAAGCATATTTACGGCGGTAGAAAATTCGGTGGGGAGCCATTTTTTTTACCGAAAGAGGGAAATTCAAAGGAAGAAGATGAAGGATACATTCTGGCGTTTGTTCATGATGAAAGGACATGGAAATCAGAGGTTCAGATAGTGAACGCCATGGATTTGAAAGTGGAAGCTACAATAAAATTACCTTCGAGAGTTCCATATGGATTTCATGGGACATTCGTTCAGTCAAACAGTTTGCAAAATCAAGCTTAA

Protein sequence

MMAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA

Homology

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BLAST of Bhi03G000083 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 837.4 bits (2162), Expect = 7.1e-243
Identity = 415/593 (69.98%), Postives = 472/593 (79.60%), Query Frame = 0


Query: 7   PPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTP 66
           PP SSS    LS+ +S                +    T    + SALH+P  LHFP Q+ 
Sbjct: 22  PPLSSSQSSDLSYCSSLP--------------MASRVTRKLNVSSALHTPPALHFPKQSS 81

Query: 67  NFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGN 126
           N    + +K +  +S     NL Q+ A+ AL   E  LVSHE  HPLPKTADP VQI+GN
Sbjct: 82  N-SPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGN 141

Query: 127 FAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGS 186
           FAPV EQPV+ NLPV G +PD I GVY+RNGANPL +P  GHH FDGDGMVHAV    GS
Sbjct: 142 FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGS 201

Query: 187 ASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGV 246
           ASYACRFTQT R VQER+LGRPVFPKAIGELHGH+GIARL+LFYAR   G+VD   GTGV
Sbjct: 202 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGV 261

Query: 247 ANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKE 306
           ANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ QL STMIAHPKVDP S E
Sbjct: 262 ANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGE 321

Query: 307 LYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKL 366
           L+ALSYDVV KPYLKYFRFSPDG KS DVEI L+ PTMMHDFAITENFVV+PDQQVVFKL
Sbjct: 322 LFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKL 381

Query: 367 QEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVV 426
            EM++GGSPV+YDK K +RFGIL K A DS ++ W+++PD FCFHLWNAWEEPET+EVVV
Sbjct: 382 PEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVV 441

Query: 427 IGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRL 486
           IGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG+STRR II  E +Q+NLEAGMVNRN L
Sbjct: 442 IGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNML 501

Query: 487 GRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEG 546
           GRKT+FAYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GGEP FLP EG   EEDEG
Sbjct: 502 GRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG--EEDEG 561

Query: 547 YILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQ 600
           YIL FVHDE+TWKSE+QIVNA+ L+VEAT+KLPSRVPYGFHGTF+ ++ L  Q
Sbjct: 562 YILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

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BLAST of Bhi03G000083 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 822.8 bits (2124), Expect = 1.8e-238
Identity = 403/577 (69.84%), Postives = 475/577 (82.32%), Query Frame = 0


Query: 25  SNFPAFNYNNNVKFINQNSTHNPI-IHSALHSPSVLHFPNQTPNFHNNLTLKDQTNKSPF 84
           S+  A + +++V F N       +  +S   +P++L+FPN  P+ +  +  KD       
Sbjct: 21  SDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDT------ 80

Query: 85  SNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTG 144
           S WN LQ+ AS AL   E+AL+  E   PLPKT DPR QISGN+APVPEQ VK +L V G
Sbjct: 81  SRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDG 140

Query: 145 TIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQRLVQER 204
            IPDCI GVYLRNGANPLF+P +GHHLFDGDGMVHAV I  G ASY+CRFT+T+RLVQE+
Sbjct: 141 KIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEK 200

Query: 205 RLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSE 264
           +LG P+FPKAIGELHGHSGIARL+LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSE
Sbjct: 201 QLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSE 260

Query: 265 DDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYF 324
           DDLPY VR+T +GDL+T+GR+DF+ QL S MIAHPK+DP++KEL+ALSYDVV+KPYLKYF
Sbjct: 261 DDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYF 320

Query: 325 RFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKK 384
           +FSP+G KS DVEIPL +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV YD +K 
Sbjct: 321 KFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKI 380

Query: 385 SRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEE 444
           SRFGILP+NA D+ +++WVESP+TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E
Sbjct: 381 SRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDE 440

Query: 445 NLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPK 504
            L SVLSEIRLNL+TGKSTRR II     QMNLEAGMVNRN LGRKTR+AYLAIAEPWPK
Sbjct: 441 QLNSVLSEIRLNLKTGKSTRRTII-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPK 500

Query: 505 VSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQ 564
           VSGFAKVDL TGEVK H YGG+K+GGEPFFLP+   S  ED+GYI++FVHDE +W+SE+ 
Sbjct: 501 VSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELH 560

Query: 565 IVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           IVNA+ L++EAT+KLPSRVPYGFHGTFV S  + NQA
Sbjct: 561 IVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

[Close]

BLAST of Bhi03G000083 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 807.7 bits (2085), Expect = 6.1e-234
Identity = 390/537 (72.63%), Postives = 458/537 (85.29%), Query Frame = 0


Query: 65  TPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQIS 124
           TP  HN L L            N+ QK A++A+   E AL+SHE   PLPKTADPRVQI+
Sbjct: 60  TPPQHNPLRL------------NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIA 119

Query: 125 GNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTING 184
           GN++PVPE  V+ NL V GTIPDCI GVY+RNGANP+F+PTAGHHLFDGDGMVHAV I  
Sbjct: 120 GNYSPVPESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITN 179

Query: 185 GSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGT 244
           GSASYACRFT+T+RLVQE+RLGRPVFPKAIGELHGHSGIARL+LFYARG+ GL++++ G 
Sbjct: 180 GSASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGV 239

Query: 245 GVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPIS 304
           GVANAGLVYFN+RLLAMSEDDLPY ++IT +GDLQTVGRYDF+ QL S MIAHPK+DP++
Sbjct: 240 GVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVT 299

Query: 305 KELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVF 364
           KEL+ALSYDVV+KPYLKYFRFSPDG+KS ++EIPLETPTM+HDFAITENFVVIPDQQVVF
Sbjct: 300 KELHALSYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVF 359

Query: 365 KLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEV 424
           KL EM+ G SPV++D +K SR GI+PK+AT++  +IWV SP+TFCFHLWNAWE PETEE+
Sbjct: 360 KLGEMISGKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEI 419

Query: 425 VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRN 484
           VVIGSCM+P DSIFNE +E+L+SVLSEIR+NLRT K+TRR+++    E +NLE GMVNRN
Sbjct: 420 VVIGSCMSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLL--VNEDVNLEIGMVNRN 479

Query: 485 RLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLP-KEGNSKE- 544
           RLGRKTRFA+LAIA PWPKVSGFAKVDL TGE+KK+IYGG K+GGEPFFLP   GN +E 
Sbjct: 480 RLGRKTRFAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEEN 539

Query: 545 EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQ 600
           ED+GYI   VHDE T  SE+QI+NA++LK+EATIKLPSRVPYGFHGTFV SN L +Q
Sbjct: 540 EDDGYIFCHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

[Close]

BLAST of Bhi03G000083 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 788.1 bits (2034), Expect = 5.0e-228
Identity = 391/607 (64.42%), Postives = 478/607 (78.75%), Query Frame = 0


Query: 7   PPSSSSLLDHL--SFSNSTRSNFPAFNYNNNVK--------FINQNSTHNPIIHSALHSP 66
           P +S+  LD    + S+S+R    + ++++ ++        +++ +      + S+L SP
Sbjct: 58  PSTSTQFLDRTFSTSSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSP 117

Query: 67  SVLHFPNQTPNFHNNLT--LKDQTNKSPFSNWNLLQKTASMALYMVESALVSHE-LQHPL 126
           +    P       N++T  +   TN++P    N +Q+TA+M L  VE+A++SHE  +HP 
Sbjct: 118 TFK--PPSWKKLCNDVTNLIPKTTNQNP--KLNPVQRTAAMVLDAVENAMISHERRRHPH 177

Query: 127 PKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDG 186
           PKTADP VQI+GNF PVPE+PV HNLPVTGT+P+CI GVY+RNGANPL +P +GHHLFDG
Sbjct: 178 PKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDG 237

Query: 187 DGMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARG 246
           DGMVHAV  + GS SYACRFT+T RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RG
Sbjct: 238 DGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRG 297

Query: 247 VFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHST 306
           +FGLVD   G GVANAGLVYFN  LLAMSEDDLPYHV++T +GDL+T GRYDF+ QL ST
Sbjct: 298 LFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKST 357

Query: 307 MIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITEN 366
           MIAHPK+DP ++EL+ALSYDVV KPYLKYFRF+ DG KS DVEIPL+ PTM+HDFAITEN
Sbjct: 358 MIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITEN 417

Query: 367 FVVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLW 426
           FVVIPDQQVVF+L EM++GGSPV+YD+KKKSRFGIL KNA D+  + W+E PD FCFHLW
Sbjct: 418 FVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLW 477

Query: 427 NAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ 486
           N+WEEPET+EVVVIGSCMTPPDSIFNE +E L+SVLSEIRLNL+TG+STRR +I    EQ
Sbjct: 478 NSWEEPETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI---SEQ 537

Query: 487 MNLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFF 546
           +NLEAGMVNRN LGRKTR+AYLA+ EPWPKVSGFAKVDL TGE++K+IYG  K+GGEP F
Sbjct: 538 VNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLF 597

Query: 547 LPKEGNSKEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQS 601
           LP      EED GYI+ FVHDE   KSE+Q++NA+++K+EAT+ LPSRVPYGFHGTF+  
Sbjct: 598 LP--SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISK 655

[Close]

BLAST of Bhi03G000083 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 630.2 bits (1624), Expect = 1.7e-180
Identity = 312/526 (59.32%), Postives = 392/526 (74.52%), Query Frame = 0


Query: 80  KSPFSNWNLLQKTASMALYMVESALV-SHELQHPLPKTADPRVQISGNFAPVPEQPVKHN 139
           K  + N NLLQK A+  L  +ES++V   E   PLPK  DP VQ+SGNFAPV E PV++ 
Sbjct: 57  KPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNG 116

Query: 140 LPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTIN-GGSASYACRFTQTQ 199
           L V G IP C+ GVY+RNGANP+F P AGHHLFDGDGM+HAV+I      SY+CR+T+T 
Sbjct: 117 LEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTN 176

Query: 200 RLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDR 259
           RLVQE  LGR VFPK IGELHGHSG+ARL LF AR   GLVD  +G GVANAG+V+FN R
Sbjct: 177 RLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGR 236

Query: 260 LLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRK 319
           LLAMSEDDLPY V+I   GDL+T+GR+ F+ Q+ S++IAHPKVD  + +L+ LSY+V++K
Sbjct: 237 LLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKK 296

Query: 320 PYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 379
           P+L+Y +F+  G K++DVEI L  PTM+HDFAITENFVVIPDQQ+VFKL EM++GGSPVI
Sbjct: 297 PHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVI 356

Query: 380 YDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEE----VVVIGSCMTP 439
           Y K+K +RFG+L K      D+ WV+ PD FCFHLWNAWEE  TEE    +VVIGSCM+P
Sbjct: 357 YVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPVIVVIGSCMSP 416

Query: 440 PDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFA 499
           PD+IF+E  E  +  LSEIRLN+RT +S R+ I+      +NLEAG +NR+ +GRK++F 
Sbjct: 417 PDTIFSESGEPTRVELSEIRLNMRTKESNRKVIV----TGVNLEAGHINRSYVGRKSQFV 476

Query: 500 YLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVH 559
           Y+AIA+PWPK SG AKVD+  G V +  YG  +FGGEP F+P EG   EED+GY++ FV 
Sbjct: 477 YIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEG-EEDKGYVMGFVR 536

Query: 560 DERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQ 600
           DE   +SE  +V+A D+K  A ++LP RVPYGFHGTFV  N L+ Q
Sbjct: 537 DEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

[Close]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 891.3 bits (2302), Expect = 5.9e-258
Identity = 427/577 (74.00%), Postives = 495/577 (85.79%), Query Frame = 0


Query: 33  NNNVKFI-NQNSTHNPIIHSALHSPSVLHFPNQTPNF----HNNLTLKDQTN----KSPF 92
           +N++  + NQ++  +  I+S+L +P +LHFP Q+ N+    +N ++   Q N     S  
Sbjct: 29  SNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSST 88

Query: 93  SNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTG 152
           S WNL+QK A+MAL  VESAL  HEL+HPLPKTADPRVQISGNFAPVPE PV  +LPVTG
Sbjct: 89  SKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTG 148

Query: 153 TIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQRLVQER 212
            IP C+ GVY+RNGANPLF+PTAGHH FDGDGMVHAV    GSASYACRFT+T+RLVQE+
Sbjct: 149 KIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEK 208

Query: 213 RLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSE 272
            LGRPVFPKAIGELHGHSGIARL+LFYARG+FGLVDH KGTGVANAGLVYFN+RLLAMSE
Sbjct: 209 ALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSE 268

Query: 273 DDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYF 332
           DDLPYHV++TP+GDL+T GR+DF+ QL STMIAHPK+DP+S EL+ALSYDV++KPYLKYF
Sbjct: 269 DDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYF 328

Query: 333 RFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKK 392
           RFS +G KS DVEIP+E PTMMHDFAITENFVVIPDQQVVFK+ EM++GGSPV+YDK K 
Sbjct: 329 RFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKV 388

Query: 393 SRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEE 452
           SRFGIL K A D  DL WVE PD FCFHLWNAWEE ET+E+VVIGSCMTPPDSIFNEC+E
Sbjct: 389 SRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDE 448

Query: 453 NLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPK 512
            LKSVLSEIRLNL+TGKSTR++II+   EQ+NLEAGMVNRN+LGRKT +AYLAIAEPWPK
Sbjct: 449 GLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPK 508

Query: 513 VSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQ 572
           VSGFAKV+L TGEV+K IYG  K+GGEP FLP++ NSKEED+GYILAFVHDE+ WKSE+Q
Sbjct: 509 VSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQ 568

Query: 573 IVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           IVNAM LK+EAT+KLPSRVPYGFHGTF+ +N L NQA
Sbjct: 569 IVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA 605

[Close]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 2.2e-257
Identity = 430/582 (73.88%), Postives = 494/582 (84.88%), Query Frame = 0


Query: 18  SFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTPNFHNNLTLKDQ 77
           SFS ST  +      + ++   N  +T   I  + L  P   + P   P F         
Sbjct: 13  SFSPSTSYSLDFTLPSKSISMKNHTTTKTKIHSALLTLPKQNNTPKNQPQFQT------- 72

Query: 78  TNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKH 137
                 S+WN  QK A+ AL +VESALVS ELQ+PLPKTADPRVQI+GNFAPVPEQ V+H
Sbjct: 73  ------SHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRH 132

Query: 138 NLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQ 197
           NLPVTGTIPDCI+GVY+RNGANPLF+P AGHHLFDGDGMVHAVT+  GS SY+CRFT+T+
Sbjct: 133 NLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETE 192

Query: 198 RLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDR 257
           RLVQER LG PVFPKAIGELHGHSGIARLLLFYARGVFGLVDH  GTGVANAGLV+FN+R
Sbjct: 193 RLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNR 252

Query: 258 LLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRK 317
           LLAMSEDD+PYHV++ PSGDLQTVGRY+F+ QL STMIAHPK+DP+S EL+ALSYDVV+K
Sbjct: 253 LLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQK 312

Query: 318 PYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVI 377
           PYLK F+FSPDG KS DVEIPL+ PTMMHDFAITEN+VVIPDQQVVFKLQEM+KGGSPVI
Sbjct: 313 PYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVI 372

Query: 378 YDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSI 437
           YDK KKSRFGILPKNA +S+++IWVES +TFCFHLWNAWEEPET+EV+VIGSCMTPPDSI
Sbjct: 373 YDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSI 432

Query: 438 FNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAI 497
           FNEC ENLKSVLSEIRLNL+TG+STRR ++    +Q+NLEAGMVNRN+LGRKT+FAYLAI
Sbjct: 433 FNECNENLKSVLSEIRLNLKTGESTRRQLL-SPSDQVNLEAGMVNRNKLGRKTQFAYLAI 492

Query: 498 AEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERT 557
           AEPWPKVSGFAKVDL TGE+KKHIYG +++GGEP FLP+  NS++ED+GYILAF HDE+T
Sbjct: 493 AEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKT 552

Query: 558 WKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQ 600
           WKSE+QIVNAM L++EAT+KLPSRVPYGFHGTF+ S  LQNQ
Sbjct: 553 WKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

[Close]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 1.0e-241
Identity = 415/593 (69.98%), Postives = 472/593 (79.60%), Query Frame = 0


Query: 7   PPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQTP 66
           PP SSS    LS+ +S                +    T    + SALH+P  LHFP Q+ 
Sbjct: 22  PPLSSSQSSDLSYCSSLP--------------MASRVTRKLNVSSALHTPPALHFPKQSS 81

Query: 67  NFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGN 126
           N    + +K +  +S     NL Q+ A+ AL   E  LVSHE  HPLPKTADP VQI+GN
Sbjct: 82  N-SPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGN 141

Query: 127 FAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGS 186
           FAPV EQPV+ NLPV G +PD I GVY+RNGANPL +P  GHH FDGDGMVHAV    GS
Sbjct: 142 FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGS 201

Query: 187 ASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGV 246
           ASYACRFTQT R VQER+LGRPVFPKAIGELHGH+GIARL+LFYAR   G+VD   GTGV
Sbjct: 202 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGV 261

Query: 247 ANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKE 306
           ANAGLVYFN RLLAMSEDDLPY V+ITP+GDL+TVGR+DF+ QL STMIAHPKVDP S E
Sbjct: 262 ANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGE 321

Query: 307 LYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKL 366
           L+ALSYDVV KPYLKYFRFSPDG KS DVEI L+ PTMMHDFAITENFVV+PDQQVVFKL
Sbjct: 322 LFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKL 381

Query: 367 QEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVV 426
            EM++GGSPV+YDK K +RFGIL K A DS ++ W+++PD FCFHLWNAWEEPET+EVVV
Sbjct: 382 PEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVV 441

Query: 427 IGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRL 486
           IGSCMTPPDSIFNE +ENLKSVLSEIRLNL+TG+STRR II  E +Q+NLEAGMVNRN L
Sbjct: 442 IGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNML 501

Query: 487 GRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEG 546
           GRKT+FAYLA+AEPWPKVSGFAKVDL TGEVKKH+YG  ++GGEP FLP EG   EEDEG
Sbjct: 502 GRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG--EEDEG 561

Query: 547 YILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQ 600
           YIL FVHDE+TWKSE+QIVNA+ L+VEAT+KLPSRVPYGFHGTF+ ++ L  Q
Sbjct: 562 YILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 8.0e-239
Identity = 396/522 (75.86%), Postives = 450/522 (86.21%), Query Frame = 0


Query: 80  KSPF-SNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHN 139
           K PF   WN LQK A+  L MVE+ALVSHE +HPLPKTADP+VQI+GNFAPVPE      
Sbjct: 98  KQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQA 157

Query: 140 LPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQR 199
           LPV G IP CI GVY+RNGANPL++P AGHH FDGDGMVHAV    G+ASYACRFT+TQR
Sbjct: 158 LPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGMVHAVKFTNGAASYACRFTETQR 217

Query: 200 LVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRL 259
           L QE+ LGRPVFPKAIGELHGHSGIARLLLFYAR +F LVD   G GVANAGLVYFN+ L
Sbjct: 218 LAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHL 277

Query: 260 LAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRKP 319
           LAMSEDDLPYHVRIT +GDL TVGRYDFN QL+STMIAHPK+DP++ +L+ALSYDVV+KP
Sbjct: 278 LAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKP 337

Query: 320 YLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIY 379
           YLKYFRFS DG+KS DVEIPL+ PTMMHDFAITENFVV+PDQQVVFKL EM+ GGSPV+Y
Sbjct: 338 YLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVY 397

Query: 380 DKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIF 439
           DK K SRFGIL KNA D+  + W+++P+ FCFHLWNAWEEPET+E+VVIGSCMTP DSIF
Sbjct: 398 DKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIF 457

Query: 440 NECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAIA 499
           NEC+E+LKSVLSEIRLNLRTGKSTRR II  + EQ+NLEAGMVNRN+LGRKT+FAYLA+A
Sbjct: 458 NECDESLKSVLSEIRLNLRTGKSTRRPII-SDAEQVNLEAGMVNRNKLGRKTQFAYLALA 517

Query: 500 EPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERTW 559
           EPWPKVSGFAKVDL +GEV+K++YG  KFGGEP FLP   N +EE +GYILAFVHDE+ W
Sbjct: 518 EPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP---NGEEEGDGYILAFVHDEKEW 577

Query: 560 KSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           KSE+QIVNA +LK+EA+IKLPSRVPYGFHGTF+ S  L+ QA
Sbjct: 578 KSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLRKQA 615

[Close]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.6e-237
Identity = 403/577 (69.84%), Postives = 475/577 (82.32%), Query Frame = 0


Query: 25  SNFPAFNYNNNVKFINQNSTHNPI-IHSALHSPSVLHFPNQTPNFHNNLTLKDQTNKSPF 84
           S+  A + +++V F N       +  +S   +P++L+FPN  P+ +  +  KD       
Sbjct: 21  SDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-PSPNPIIPEKDT------ 80

Query: 85  SNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRVQISGNFAPVPEQPVKHNLPVTG 144
           S WN LQ+ AS AL   E+AL+  E   PLPKT DPR QISGN+APVPEQ VK +L V G
Sbjct: 81  SRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDG 140

Query: 145 TIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVTINGGSASYACRFTQTQRLVQER 204
            IPDCI GVYLRNGANPLF+P +GHHLFDGDGMVHAV I  G ASY+CRFT+T+RLVQE+
Sbjct: 141 KIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEK 200

Query: 205 RLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSE 264
           +LG P+FPKAIGELHGHSGIARL+LFYARG+FGL++HK GTGVANAGLVYF+DRLLAMSE
Sbjct: 201 QLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSE 260

Query: 265 DDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVDPISKELYALSYDVVRKPYLKYF 324
           DDLPY VR+T +GDL+T+GR+DF+ QL S MIAHPK+DP++KEL+ALSYDVV+KPYLKYF
Sbjct: 261 DDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYF 320

Query: 325 RFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKK 384
           +FSP+G KS DVEIPL +PTMMHDFAITENFVVIPDQQVVFKL +M  G SPV YD +K 
Sbjct: 321 KFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKI 380

Query: 385 SRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPETEEVVVIGSCMTPPDSIFNECEE 444
           SRFGILP+NA D+ +++WVESP+TFCFHLWNAWE PET+EVVVIGSCMTP DSIFNEC+E
Sbjct: 381 SRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDE 440

Query: 445 NLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPK 504
            L SVLSEIRLNL+TGKSTRR II     QMNLEAGMVNRN LGRKTR+AYLAIAEPWPK
Sbjct: 441 QLNSVLSEIRLNLKTGKSTRRTII-PGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPK 500

Query: 505 VSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQ 564
           VSGFAKVDL TGEVK H YGG+K+GGEPFFLP+   S  ED+GYI++FVHDE +W+SE+ 
Sbjct: 501 VSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELH 560

Query: 565 IVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           IVNA+ L++EAT+KLPSRVPYGFHGTFV S  + NQA
Sbjct: 561 IVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

[Close]

BLAST of Bhi03G000083 vs. NCBI nr
Match: XP_038883946.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0


Query: 1   MMAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLH 60
           MMAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLH
Sbjct: 1   MMAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLH 60

Query: 61  FPNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPR 120
           FPNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPR
Sbjct: 61  FPNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPR 120

Query: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180
           VQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAV
Sbjct: 121 VQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAV 180

Query: 181 TINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240
           TINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH
Sbjct: 181 TINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDH 240

Query: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKV 300
           KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKV
Sbjct: 241 KKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKV 300

Query: 301 DPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360
           DPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420
           QVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE
Sbjct: 361 QVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPE 420

Query: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGM 480
           TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGM
Sbjct: 421 TEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGM 480

Query: 481 VNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNS 540
           VNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNS
Sbjct: 481 VNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNS 540

Query: 541 KEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600
           KEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA
Sbjct: 541 KEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 600

[Close]

BLAST of Bhi03G000083 vs. NCBI nr
Match: XP_004134911.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN49403.1 hypothetical protein Csa_004240 [Cucumis sativus])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 551/610 (90.33%), Postives = 566/610 (92.79%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           M ASP P    LLDHL F NST+SNFP FN NN     +QNS    IIHSALHSPSVLHF
Sbjct: 1   MTASPSP----LLDHLGFLNSTKSNFPNFNINNLKPITSQNS----IIHSALHSPSVLHF 60

Query: 62  PNQ----------TPNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQ 121
           PNQ          TPNFH +L+LK Q  KSP + NWNLLQKTASMAL MVESALVSHELQ
Sbjct: 61  PNQTTTSTTTTSTTPNFHKSLSLKHQ--KSPTAPNWNLLQKTASMALDMVESALVSHELQ 120

Query: 122 HPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHL 181
           HPLPKTADPRVQISGNFAPVPEQ VKH LPV GTIPDCI+GVYLRNGANPLFQPTAGHHL
Sbjct: 121 HPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHL 180

Query: 182 FDGDGMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 241
           FDGDGMVHAV+IN GSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY
Sbjct: 181 FDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 240

Query: 242 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQL 301
           ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL
Sbjct: 241 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQL 300

Query: 302 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAI 361
           HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIPLETPTMMHDFAI
Sbjct: 301 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAI 360

Query: 362 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCF 421
           TENFVVIPDQQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCF
Sbjct: 361 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCF 420

Query: 422 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 481
           HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE
Sbjct: 421 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 480

Query: 482 KEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 541
           KEQ+NLEAGMVN+NR+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE
Sbjct: 481 KEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 540

Query: 542 PFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTF 601
           PFFLPKE NSKEEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTF
Sbjct: 541 PFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTF 600

[Close]

BLAST of Bhi03G000083 vs. NCBI nr
Match: XP_008439625.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 545/609 (89.49%), Postives = 566/609 (92.94%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           M A P P    LLDHL F NST+SNFP    N N+  +   +T N IIHSALHSPSVL F
Sbjct: 1   MTAPPSP----LLDHLGFLNSTKSNFP----NLNINHLKSITTQNSIIHSALHSPSVLRF 60

Query: 62  PNQT------PNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQHPLP 121
           PNQT      PNFH +L+LK Q  KSP + NWNLLQKTAS+AL MVESALVSHELQHPLP
Sbjct: 61  PNQTTTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLP 120

Query: 122 KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGD 181
           KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLFQPTAGHHLFDGD
Sbjct: 121 KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGD 180

Query: 182 GMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 241
           GMVHAVTIN GSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGV
Sbjct: 181 GMVHAVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGV 240

Query: 242 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTM 301
           FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQLHSTM
Sbjct: 241 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTM 300

Query: 302 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENF 361
           IAHPKVDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIP+ETPTMMHDFAITENF
Sbjct: 301 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENF 360

Query: 362 VVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 421
           VVIPDQQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCFHLWN
Sbjct: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420

Query: 422 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQM 481
           AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ+
Sbjct: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480

Query: 482 NLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 541
           NLEAGMVN+ R+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL
Sbjct: 481 NLEAGMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540

Query: 542 PKEGNSK---EEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFV 601
           PK+ NSK   EEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV
Sbjct: 541 PKDENSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFV 599

[Close]

BLAST of Bhi03G000083 vs. NCBI nr
Match: KAA0052537.1 (9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 544/604 (90.07%), Postives = 566/604 (93.71%), Query Frame = 0


Query: 7   PPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQT- 66
           PP  S+LLDHL F NST+SNFP    N N+  +   +T N IIHSALHSPSVL FPNQT 
Sbjct: 4   PP--SALLDHLGFLNSTKSNFP----NLNINHLKSITTQNSIIHSALHSPSVLRFPNQTT 63

Query: 67  -----PNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQHPLPKTADP 126
                PNFH +L+LK Q  KSP + NWNLLQKTAS+AL MVESALVSHELQHPLPKTADP
Sbjct: 64  TTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADP 123

Query: 127 RVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHA 186
           RVQISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLFQPTAGHHLFDGDGMVHA
Sbjct: 124 RVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHA 183

Query: 187 VTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVD 246
           VTIN GSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVD
Sbjct: 184 VTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVD 243

Query: 247 HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPK 306
           HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQLHSTMIAHPK
Sbjct: 244 HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPK 303

Query: 307 VDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPD 366
           VDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIP+ETPTMMHDFAITENFVVIPD
Sbjct: 304 VDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPD 363

Query: 367 QQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP 426
           QQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP
Sbjct: 364 QQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP 423

Query: 427 ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAG 486
           ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ+NLEAG
Sbjct: 424 ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAG 483

Query: 487 MVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGN 546
           MVN+ R+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ N
Sbjct: 484 MVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDEN 543

Query: 547 SK---EEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSL 601
           SK   EEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTFVQSN+L
Sbjct: 544 SKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNL 599

[Close]

BLAST of Bhi03G000083 vs. NCBI nr
Match: KAG6581433.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034725.1 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1057.0 bits (2732), Expect = 6.2e-305
Identity = 523/600 (87.17%), Postives = 552/600 (92.00%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           MAAS P  S   LD+L F NST+S     N   NV  I Q S HN +IHSALHSPSVLHF
Sbjct: 1   MAASSP--SPPCLDYLGFHNSTKS----INGFKNVNLIRQPSNHNAVIHSALHSPSVLHF 60

Query: 62  PNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRV 121
           PN +PNFH+NL       KSP SNWN LQK+AS+AL M ESAL+SHEL+HPLPKTADPRV
Sbjct: 61  PNHSPNFHDNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 120

Query: 122 QISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVT 181
           QISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVT
Sbjct: 121 QISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 180

Query: 182 INGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 241
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 181 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 240

Query: 242 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVD 301
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL STMIAHPKVD
Sbjct: 241 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 300

Query: 302 PISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 361
           P+S+ELYALSYDVVRKPYLKYFRFSPDG+KSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 301 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 360

Query: 362 VVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 421
           VVFKLQEM+KGGSPVIYDK KKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 361 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 420

Query: 422 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMV 481
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQ+NLEAGMV
Sbjct: 421 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 480

Query: 482 NRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSK 541
           NRNRLGRKTR+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNS 
Sbjct: 481 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 540

Query: 542 E-EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV +  LQNQA
Sbjct: 541 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

[Close]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL
Match: A0A0A0KIL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 551/610 (90.33%), Postives = 566/610 (92.79%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           M ASP P    LLDHL F NST+SNFP FN NN     +QNS    IIHSALHSPSVLHF
Sbjct: 1   MTASPSP----LLDHLGFLNSTKSNFPNFNINNLKPITSQNS----IIHSALHSPSVLHF 60

Query: 62  PNQ----------TPNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQ 121
           PNQ          TPNFH +L+LK Q  KSP + NWNLLQKTASMAL MVESALVSHELQ
Sbjct: 61  PNQTTTSTTTTSTTPNFHKSLSLKHQ--KSPTAPNWNLLQKTASMALDMVESALVSHELQ 120

Query: 122 HPLPKTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHL 181
           HPLPKTADPRVQISGNFAPVPEQ VKH LPV GTIPDCI+GVYLRNGANPLFQPTAGHHL
Sbjct: 121 HPLPKTADPRVQISGNFAPVPEQAVKHYLPVAGTIPDCINGVYLRNGANPLFQPTAGHHL 180

Query: 182 FDGDGMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 241
           FDGDGMVHAV+IN GSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY
Sbjct: 181 FDGDGMVHAVSINHGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFY 240

Query: 242 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQL 301
           ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQL
Sbjct: 241 ARGVFGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQL 300

Query: 302 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAI 361
           HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIPLETPTMMHDFAI
Sbjct: 301 HSTMIAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPLETPTMMHDFAI 360

Query: 362 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCF 421
           TENFVVIPDQQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCF
Sbjct: 361 TENFVVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCF 420

Query: 422 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 481
           HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE
Sbjct: 421 HLWNAWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEE 480

Query: 482 KEQMNLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 541
           KEQ+NLEAGMVN+NR+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE
Sbjct: 481 KEQVNLEAGMVNKNRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGE 540

Query: 542 PFFLPKEGNSKEEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTF 601
           PFFLPKE NSKEEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTF
Sbjct: 541 PFFLPKEENSKEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTF 600

[Close]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL
Match: A0A5A7UDK3 (9-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 544/604 (90.07%), Postives = 566/604 (93.71%), Query Frame = 0


Query: 7   PPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHFPNQT- 66
           PP  S+LLDHL F NST+SNFP    N N+  +   +T N IIHSALHSPSVL FPNQT 
Sbjct: 4   PP--SALLDHLGFLNSTKSNFP----NLNINHLKSITTQNSIIHSALHSPSVLRFPNQTT 63

Query: 67  -----PNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQHPLPKTADP 126
                PNFH +L+LK Q  KSP + NWNLLQKTAS+AL MVESALVSHELQHPLPKTADP
Sbjct: 64  TTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLPKTADP 123

Query: 127 RVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHA 186
           RVQISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLFQPTAGHHLFDGDGMVHA
Sbjct: 124 RVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGDGMVHA 183

Query: 187 VTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVD 246
           VTIN GSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGVFGLVD
Sbjct: 184 VTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGVFGLVD 243

Query: 247 HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPK 306
           HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQLHSTMIAHPK
Sbjct: 244 HKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTMIAHPK 303

Query: 307 VDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPD 366
           VDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIP+ETPTMMHDFAITENFVVIPD
Sbjct: 304 VDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENFVVIPD 363

Query: 367 QQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP 426
           QQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP
Sbjct: 364 QQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEP 423

Query: 427 ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAG 486
           ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ+NLEAG
Sbjct: 424 ETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQVNLEAG 483

Query: 487 MVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGN 546
           MVN+ R+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPK+ N
Sbjct: 484 MVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKDEN 543

Query: 547 SK---EEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSL 601
           SK   EEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTFVQSN+L
Sbjct: 544 SKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFVQSNNL 599

[Close]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL
Match: A0A1S3AZU0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103484361 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 545/609 (89.49%), Postives = 566/609 (92.94%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           M A P P    LLDHL F NST+SNFP    N N+  +   +T N IIHSALHSPSVL F
Sbjct: 1   MTAPPSP----LLDHLGFLNSTKSNFP----NLNINHLKSITTQNSIIHSALHSPSVLRF 60

Query: 62  PNQT------PNFHNNLTLKDQTNKSPFS-NWNLLQKTASMALYMVESALVSHELQHPLP 121
           PNQT      PNFH +L+LK Q  KSP + NWNLLQKTAS+AL MVESALVSHELQHPLP
Sbjct: 61  PNQTTTTPPPPNFHKSLSLKHQ--KSPTAPNWNLLQKTASIALDMVESALVSHELQHPLP 120

Query: 122 KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGD 181
           KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLFQPTAGHHLFDGD
Sbjct: 121 KTADPRVQISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFQPTAGHHLFDGD 180

Query: 182 GMVHAVTINGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGV 241
           GMVHAVTIN GSASYACRFTQTQRLVQERRLGRP+FPKAIGELHGHSGIARLLLFYARGV
Sbjct: 181 GMVHAVTINHGSASYACRFTQTQRLVQERRLGRPIFPKAIGELHGHSGIARLLLFYARGV 240

Query: 242 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTM 301
           FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDF+KQLHSTM
Sbjct: 241 FGLVDHKKGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFDKQLHSTM 300

Query: 302 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENF 361
           IAHPKVDPISKELYALSYDVVRKPYLKYFRFSP+G+KSKDVEIP+ETPTMMHDFAITENF
Sbjct: 301 IAHPKVDPISKELYALSYDVVRKPYLKYFRFSPNGIKSKDVEIPVETPTMMHDFAITENF 360

Query: 362 VVIPDQQVVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 421
           VVIPDQQVVFKLQEMVKGGSPVIYDK KKSRFGILPKNATDSKDLIWVESPDTFCFHLWN
Sbjct: 361 VVIPDQQVVFKLQEMVKGGSPVIYDKNKKSRFGILPKNATDSKDLIWVESPDTFCFHLWN 420

Query: 422 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQM 481
           AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQ+
Sbjct: 421 AWEEPETEEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQV 480

Query: 482 NLEAGMVNRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 541
           NLEAGMVN+ R+GRK+R+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL
Sbjct: 481 NLEAGMVNKTRVGRKSRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL 540

Query: 542 PKEGNSK---EEDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFV 601
           PK+ NSK   EEDEGYILAFVHDERTWKSEVQIVNA DLKVEATIKLPSRVPYGFHGTFV
Sbjct: 541 PKDENSKAEEEEDEGYILAFVHDERTWKSEVQIVNAKDLKVEATIKLPSRVPYGFHGTFV 599

[Close]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL
Match: A0A6J1EH85 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433338 PE=3 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 3.9e-305
Identity = 523/600 (87.17%), Postives = 552/600 (92.00%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           MAAS P  S   LD+L F NST+S     N   NV  I Q S HN +IHSALHSPSVLHF
Sbjct: 1   MAASSP--SPPCLDYLGFHNSTKS----INGFKNVNHIRQPSNHNAVIHSALHSPSVLHF 60

Query: 62  PNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRV 121
           PN +PNFH+NL       KSP SNWN LQK+AS+AL M ESAL+SHEL+HPLPKTADPRV
Sbjct: 61  PNHSPNFHDNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 120

Query: 122 QISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVT 181
           QISGNFAPVPEQPVKHNLPVTGTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVT
Sbjct: 121 QISGNFAPVPEQPVKHNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 180

Query: 182 INGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 241
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 181 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 240

Query: 242 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVD 301
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL STMIAHPKVD
Sbjct: 241 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 300

Query: 302 PISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 361
           P+S+ELYALSYDVVRKPYLKYFRFSPDG+KSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 301 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 360

Query: 362 VVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 421
           VVFKLQEM+KGGSPVIYDK KKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 361 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 420

Query: 422 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMV 481
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQ+NLEAGMV
Sbjct: 421 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 480

Query: 482 NRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSK 541
           NRNRLGRKTR+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNS 
Sbjct: 481 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 540

Query: 542 E-EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTFV +  LQNQA
Sbjct: 541 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFVDAKHLQNQA 588

[Close]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL
Match: A0A6J1IVE0 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478741 PE=3 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.5e-304
Identity = 521/600 (86.83%), Postives = 552/600 (92.00%), Query Frame = 0


Query: 2   MAASPPPSSSSLLDHLSFSNSTRSNFPAFNYNNNVKFINQNSTHNPIIHSALHSPSVLHF 61
           MAAS P  S   LD+L F NST+S     N   NV  I Q S HN +IHSALHSPSVLHF
Sbjct: 1   MAASSP--SPPCLDYLGFHNSTKS----INGFKNVNHIRQPSNHNAVIHSALHSPSVLHF 60

Query: 62  PNQTPNFHNNLTLKDQTNKSPFSNWNLLQKTASMALYMVESALVSHELQHPLPKTADPRV 121
           PN +PNFH+NL       KSP SNWN LQK+AS+AL M ESAL+SHEL+HPLPKTADPRV
Sbjct: 61  PNHSPNFHDNLI------KSPNSNWNFLQKSASIALDMAESALLSHELRHPLPKTADPRV 120

Query: 122 QISGNFAPVPEQPVKHNLPVTGTIPDCIHGVYLRNGANPLFQPTAGHHLFDGDGMVHAVT 181
           QISGNFAPVPEQPVK+NLPVTGTIPDCI+GVYLRNGANPLF+PTAGHHLFDGDGMVHAVT
Sbjct: 121 QISGNFAPVPEQPVKYNLPVTGTIPDCINGVYLRNGANPLFEPTAGHHLFDGDGMVHAVT 180

Query: 182 INGGSASYACRFTQTQRLVQERRLGRPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHK 241
           IN GSASYACRFTQTQRL QE++LGRP+FPKAIGELHGHSGIARLLLFYARG+FGLVDHK
Sbjct: 181 INDGSASYACRFTQTQRLAQEQQLGRPIFPKAIGELHGHSGIARLLLFYARGMFGLVDHK 240

Query: 242 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLQTVGRYDFNKQLHSTMIAHPKVD 301
           KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDL+TVGRYDFN QL STMIAHPKVD
Sbjct: 241 KGTGVANAGLVYFNDRLLAMSEDDLPYHVRITPSGDLETVGRYDFNTQLDSTMIAHPKVD 300

Query: 302 PISKELYALSYDVVRKPYLKYFRFSPDGMKSKDVEIPLETPTMMHDFAITENFVVIPDQQ 361
           P+S+ELYALSYDVVRKPYLKYFRFSPDG+KSKDVEIPL+TPTMMHDFAITENFVVIPDQQ
Sbjct: 301 PVSRELYALSYDVVRKPYLKYFRFSPDGIKSKDVEIPLQTPTMMHDFAITENFVVIPDQQ 360

Query: 362 VVFKLQEMVKGGSPVIYDKKKKSRFGILPKNATDSKDLIWVESPDTFCFHLWNAWEEPET 421
           VVFKLQEM+KGGSPVIYDK KKSRFGILPKNAT+S DLIWVESPDTFCFHLWNAWEEPET
Sbjct: 361 VVFKLQEMLKGGSPVIYDKNKKSRFGILPKNATNSDDLIWVESPDTFCFHLWNAWEEPET 420

Query: 422 EEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLRTGKSTRRAIIKEEKEQMNLEAGMV 481
           EEVVVIGSCMTPPDSIFNEC+ENLKSVLSEIRLNLRTGKSTRR II +EKEQ+NLEAGMV
Sbjct: 421 EEVVVIGSCMTPPDSIFNECDENLKSVLSEIRLNLRTGKSTRRPIISDEKEQVNLEAGMV 480

Query: 482 NRNRLGRKTRFAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLPKEGNSK 541
           NRNRLGRKTR+AYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFL KEGNS 
Sbjct: 481 NRNRLGRKTRYAYLAIAEPWPKVSGFAKVDLVTGEVKKHIYGGRKFGGEPFFLQKEGNSG 540

Query: 542 E-EDEGYILAFVHDERTWKSEVQIVNAMDLKVEATIKLPSRVPYGFHGTFVQSNSLQNQA 601
           E EDEGYILAFVHDERTWKSE+QIVNAMDLKVEAT+KLPSRVPYGFHGTF+ +  LQNQA
Sbjct: 541 EKEDEGYILAFVHDERTWKSELQIVNAMDLKVEATVKLPSRVPYGFHGTFIDAKHLQNQA 588

The following BLAST results are available for this feature:

BLAST of Bhi03G000083 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14440.1	7.1e-243	69.98	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT1G30100.1	1.8e-238	69.84	nine-cis-epoxycarotenoid dioxygenase 5	[more]
AT4G18350.1	6.1e-234	72.63	nine-cis-epoxycarotenoid dioxygenase 2	[more]
AT1G78390.1	5.0e-228	64.42	nine-cis-epoxycarotenoid dioxygenase 9	[more]
AT3G24220.1	1.7e-180	59.32	nine-cis-epoxycarotenoid dioxygenase 6	[more]

BLAST of Bhi03G000083 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24023	5.9e-258	74.00	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O...	[more]
K4CJJ1	2.2e-257	73.88	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O...	[more]
Q9LRR7	1.0e-241	69.98	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O...	[more]
Q9M6E8	8.0e-239	75.86	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=...	[more]
Q9C6Z1	2.6e-237	69.84	Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t...	[more]

BLAST of Bhi03G000083 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883946.1	0.0e+00	100.00	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic-like [Benincasa hispida]	[more]
XP_004134911.1	0.0e+00	90.33	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Cucumis sativus] >KGN494...	[more]
XP_008439625.1	0.0e+00	89.49	PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ...	[more]
KAA0052537.1	0.0e+00	90.07	9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa]	[more]
KAG6581433.1	6.2e-305	87.17	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyr...	[more]

BLAST of Bhi03G000083 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIL6	0.0e+00	90.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523440 PE=3 SV=1	[more]
A0A5A7UDK3	0.0e+00	90.07	9-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3AZU0	0.0e+00	89.49	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3...	[more]
A0A6J1EH85	3.9e-305	87.17	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat...	[more]
A0A6J1IVE0	2.5e-304	86.83	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 128..591 e-value: 8.2E-116 score: 387.9
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 56..600
None	No IPR available	PANTHER	PTHR10543:SF123	9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED	coord: 56..600

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000083	Bhi03M000083	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen