Bhi03G000076 (gene) Wax gourd (B227) v1

Overview


Name	Bhi03G000076
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	gibberellin 2-beta-dioxygenase-like
Location	chr3: 2011239 .. 2012857 (+)
RNA-Seq Expression	Bhi03G000076
Synteny	Bhi03G000076

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGTAAAGAAGATAGAAACAGAGTCCCCCTTCTAAGGTATAAATACCAAGCAAACGAAGTGTTCTTCGCTGGTTCCATCCGAGTTTCGTTCTTCATTCTTCTCCCATTAAAATCCCTTAAATTCCCTCTCAATATCCTCTCCAATTCGAATTCAATCTTTTAGAAATCCATAGAATCCGCCATGGTTGTTCTGTCTCAGCCAACATTAGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCCGATATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCCATATTATCAAAGCTTGCGAAGAATTCGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCTGTCGAGTTGATGACTAAACTCGAAGATGAATCCCTCTGTTTTTTTAAACTTTCAGAGTCTGAGAAAGATAAAGCTCGCCCTCCTCACCCTTTAGGCTATGGCTGTAAAAACATCGGCTCCAATGGCGATAAGGGTTGGATTGAATATCTCCTCCTTAACGCTAATCCTCTCCCCATTTTATCCCAAAACTCTTTCCTGTAAGTTGCAAGAAAAAGCAGAGAACTCTGTTTTTTTCGTAATCCCATGTCTTTATAAGTTCACAATACGTTATTCATCTTTGTAAAACGGAACAATCTGTTTTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTCCCTTTTTTTAATTTGTGCGGATGTTGTTGCAGCTGTGCGGCTGAAGAGTATGTGAGAGCGGTGAAGAAGCTAAGCAGTGAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGGCATAAGCAAGCTTCTAAACGACGAGAAAGCAGATTGCTGTTTCAGAGTAAACCACTATCCGCCATGTCCAGAAATGCAATCTTTGGGCGGACGAAACATGATTGGATTCGGAGAACACACAGACCCACAAATCATATCGGTTTTAAGATCAAACAACTCAAGCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCCGGGGCCCTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGAAGATTCAAAAGTGTGAAGCATAAAGTGGTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTACGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACTAAATTGGCTGATTATAGGCTTGGTGCCTTTGAAAAGAGTCCAAATTCTTAAGACTTTTTTTTAAAAAAAATATATAAATTTGTTTCGTTGACCAAACTTTAATTCCCTTTCCATAAATAGCAATTCGTATAGGGATTTTCTTGTTTGTGAAGGAATTATTGAAGGGAGACTCACACAATGCTGCCTTTGATAATCTTATATGGTTAGATGTATTTCCAACGTATATTTAATGGTAAGTAACAACTGTTATATTTTATTATCATAAAATACTTTGAATCTGTTATTTGTCACTTCAAATAATTAAGTATAGGATTTTGGAAGATAATACTCCCGA

mRNA sequence

TAAGTAAAGAAGATAGAAACAGAGTCCCCCTTCTAAGGTATAAATACCAAGCAAACGAAGTGTTCTTCGCTGGTTCCATCCGAGTTTCGTTCTTCATTCTTCTCCCATTAAAATCCCTTAAATTCCCTCTCAATATCCTCTCCAATTCGAATTCAATCTTTTAGAAATCCATAGAATCCGCCATGGTTGTTCTGTCTCAGCCAACATTAGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCCGATATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCCATATTATCAAAGCTTGCGAAGAATTCGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCTGTCGAGTTGATGACTAAACTCGAAGATGAATCCCTCTGTTTTTTTAAACTTTCAGAGTCTGAGAAAGATAAAGCTCGCCCTCCTCACCCTTTAGGCTATGGCTGTAAAAACATCGGCTCCAATGGCGATAAGGGTTGGATTGAATATCTCCTCCTTAACGCTAATCCTCTCCCCATTTTATCCCAAAACTCTTTCCTCTGTGCGGCTGAAGAGTATGTGAGAGCGGTGAAGAAGCTAAGCAGTGAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGGCATAAGCAAGCTTCTAAACGACGAGAAAGCAGATTGCTGTTTCAGAGTAAACCACTATCCGCCATGTCCAGAAATGCAATCTTTGGGCGGACGAAACATGATTGGATTCGGAGAACACACAGACCCACAAATCATATCGGTTTTAAGATCAAACAACTCAAGCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCCGGGGCCCTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGAAGATTCAAAAGTGTGAAGCATAAAGTGGTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTACGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACTAAATTGGCTGATTATAGGCTTGGTGCCTTTGAAAAGAGTCCAAATTCTTAAGACTTTTTTTTAAAAAAAATATATAAATTTGTTTCGTTGACCAAACTTTAATTCCCTTTCCATAAATAGCAATTCGTATAGGGATTTTCTTGTTTGTGAAGGAATTATTGAAGGGAGACTCACACAATGCTGCCTTTGATAATCTTATATGGTTAGATGTATTTCCAACGTATATTTAATGGTAAGTAACAACTGTTATATTTTATTATCATAAAATACTTTGAATCTGTTATTTGTCACTTCAAATAATTAAGTATAGGATTTTGGAAGATAATACTCCCGA

Coding sequence (CDS)

ATGGTTGTTCTGTCTCAGCCAACATTAGATTTGAACCAATATTCTCTTCTCCGATCAACATGCAAACCCACCGCCTCCTTCGCCGATATTCCTGAAATTGACCTCTCAGATCCAAACGCCAAGTCCCATATTATCAAAGCTTGCGAAGAATTCGGCTTCTTCAAGCTCGTAAACCACCGTGTTCCTGTCGAGTTGATGACTAAACTCGAAGATGAATCCCTCTGTTTTTTTAAACTTTCAGAGTCTGAGAAAGATAAAGCTCGCCCTCCTCACCCTTTAGGCTATGGCTGTAAAAACATCGGCTCCAATGGCGATAAGGGTTGGATTGAATATCTCCTCCTTAACGCTAATCCTCTCCCCATTTTATCCCAAAACTCTTTCCTCTGTGCGGCTGAAGAGTATGTGAGAGCGGTGAAGAAGCTAAGCAGTGAAGTGGTGGAATTAATAGCAGAAGGATTAAAAATGGAGCCAAGAAACGGCATAAGCAAGCTTCTAAACGACGAGAAAGCAGATTGCTGTTTCAGAGTAAACCACTATCCGCCATGTCCAGAAATGCAATCTTTGGGCGGACGAAACATGATTGGATTCGGAGAACACACAGACCCACAAATCATATCGGTTTTAAGATCAAACAACTCAAGCGGCTTACAAATCTGCCTCAGAGACGGCGCGTGGGTTTCAGTGCCGCCGGACGCCGGGGCCCTCTTTGTGAATGTCGGCGACGCGCTGCAGGTGATGACTAACGGAAGATTCAAAAGTGTGAAGCATAAAGTGGTGGCGGATCCGAACAGAGAGAGAGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTACGCCATTGCCGCAGCTTTTAGAAGAGGGAGAAGAAAGCTTGTACAAGGAATTCACTTGGTGGGAATACAAAACGGCGGCGTATAAGACTAAATTGGCTGATTATAGGCTTGGTGCCTTTGAAAAGAGTCCAAATTCTTAA

Protein sequence

MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLPILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFTWWEYKTAAYKTKLADYRLGAFEKSPNS

Homology

[Close]

BLAST of Bhi03G000076 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 393.7 bits (1010), Expect = 1.5e-109
Identity = 197/336 (58.63%), Postives = 248/336 (73.81%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFA--DIPEIDLSDPNAKSHIIKACEEFGFFKLVN 60
           MVVL QP    N  SL+  T KP        IP ++L+DP AK+ I+KACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A PP P GYG K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPILSQNS---------FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEK 180
                + S         F  + EEY++ +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN++GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLE 300
           PD  + F+NVGDALQVMTNGRFKSVKH+V+AD  R R+SMIYFGGPPLS+KI PLP L+ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADYRLGAFEKSP 326
           E ++ LYKEFTW +YK++AYK+KL DYRLG FEK P
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQP 333

[Close]

BLAST of Bhi03G000076 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 361.7 bits (927), Expect = 6.3e-100
Identity = 182/336 (54.17%), Postives = 237/336 (70.54%), Query Frame = 0


Query: 1   MVVLSQP-TLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNH 60
           MV++ QP + D N Y  +   CKP      IP IDL+D +AK+ I+KACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPL 120
            V  +L+T+LE E++ FF L  S KDKA PP P GYG K IG NGD GW+EY+LLNAN L
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN-L 120

Query: 121 PILSQNS----------FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEK 180
            + S  +          F  A EEY++ +K++SS+ +E++ E LK+EP+  +S+L+  ++
Sbjct: 121 CLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKE 180

Query: 181 ADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVP 240
           +D C R+NHY   PE +    +  IGFGEHTDPQ+IS+LRSN++ GLQIC++DG WV V 
Sbjct: 181 SDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLE 300
           PD  + FV VGD LQVMTNGRFKSVKH+VV +  R R+SMIYF GPPLSEKI PL  L+ 
Sbjct: 241 PDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADYRLGAFEKSP 326
           + ++ LY EFTW +YK +AYKTKL DYRLG FEK P
Sbjct: 301 KQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEKRP 328

[Close]

BLAST of Bhi03G000076 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 350.9 bits (899), Expect = 1.1e-96
Identity = 168/312 (53.85%), Postives = 228/312 (73.08%), Query Frame = 0


Query: 23  PTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSES 82
           P + F+ IP ID+SDP +K  ++KACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNAN---------PLPILSQNSFLCAAEE 142
           EK +    +P GYG   IG NGD GW+EYLL+NAN         P  + S  +F  A EE
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYPPCP--EMQSLGGR 202
           Y  +V+K++ +V+E I +GL ++PRN +SKL++D+  D   R+NHYPPCP    ++ GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIISVLRSNN+SGLQI L DG+W+SVPPD  + F NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFTWWEYKTAAYKT 322
           KSV+H+V+A+  + RVSMIYF GP L+++I PL  L++  +E LY+EFTW EYK + Y +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADYRLGAFEK 324
           +L+D RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of Bhi03G000076 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 273.5 bits (698), Expect = 2.2e-73
Identity = 143/321 (44.55%), Postives = 199/321 (61.99%), Query Frame = 0


Query: 17  LRSTCKPTASFAD--IPEID--LSDPNAKSH-IIKACEEFGFFKLVNHRVPVELMTKLED 76
           L++T K T S  +   P ID  L+D +  S  I+KACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  ESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLPILSQN------ 136
           E   FF   ES+K +A P  P GYGCKNIG NGD G +EYLLL+ANP  +  ++      
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ---SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYPPC 196
               F  A  +Y+R V+ L+ E+++L  E L  +  + +S+L+ D ++D   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVGDA 256
           P   +L G   IGFGEH+DPQI++VLRSN+  GL+IC RDG W+ +P D    FV VGD 
Sbjct: 189 P--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDC 248

Query: 257 LQVMTNGRFKSVKHKVVAD-PNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFTW 316
           LQ +TNGRF SV+H+V+A+   + R+S +YF  PPL  KI+PLP+++       Y  FTW
Sbjct: 249 LQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTW 308

Query: 317 WEYKTAAYKTKLADYRLGAFE 323
            +YK A Y  +L   RL  F+
Sbjct: 309 GDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of Bhi03G000076 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-73
Identity = 140/256 (54.69%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFA--DIPEIDLSDPNAKSHIIKACEEFGFFKLVN 60
           MVVL QP    N  SL+  T KP        IP ++L+DP AK+ I+KACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A PP P GYG K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPILSQNS---------FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEK 180
                + S         F  + EEY++ +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN++GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGALFVNVGDALQV 246
           PD  + F+NVGDALQV
Sbjct: 241 PDHSSFFINVGDALQV 253

[Close]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 9.4e-117
Identity = 210/333 (63.06%), Postives = 252/333 (75.68%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQP   LNQ+ LL+   K T  F  IP +DL+ P+AK+ I+ AC +FGFFKLVNH 
Sbjct: 1   MVVLSQPA--LNQFFLLK-PFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANP-- 120
           VP+ELM  LE+E+L FFK S+SEKD+A PP P GYG K IG NGD GW+EYLLLN NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPILSQN--SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKAD 180
                L I  +N   F    E Y+ AVK +   V+EL+AEGL +  RN +S+LL DEK+D
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPD 240
            CFR+NHYPPCPE+Q+L  RN++GFGEHTDPQIISVLRSN++SGLQICL DG WVSVPPD
Sbjct: 181 SCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPD 240

Query: 241 AGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEG 300
             + F+NVGDALQVMTNGRFKSVKH+V+AD  + R+SMIYFGGP LSE I PLP ++ +G
Sbjct: 241 QTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKG 300

Query: 301 EESLYKEFTWWEYKTAAYKTKLADYRLGAFEKS 325
           EE LYKEFTW EYK AAY ++LAD RL  F+KS
Sbjct: 301 EECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

[Close]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.1e-108
Identity = 197/336 (58.63%), Postives = 248/336 (73.81%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFA--DIPEIDLSDPNAKSHIIKACEEFGFFKLVN 60
           MVVL QP    N  SL+  T KP        IP ++L+DP AK+ I+KACEEFGFFK+VN
Sbjct: 1   MVVLPQPVTLDNHISLI-PTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L +S K++A PP P GYG K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPILSQNS---------FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEK 180
                + S         F  + EEY++ +K++S +V+E++AE L +EPR+ +SK+L DEK
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVP 240
           +D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNN++GLQIC++DG+WV+VP
Sbjct: 181 SDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDAGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLE 300
           PD  + F+NVGDALQVMTNGRFKSVKH+V+AD  R R+SMIYFGGPPLS+KI PLP L+ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADYRLGAFEKSP 326
           E ++ LYKEFTW +YK++AYK+KL DYRLG FEK P
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQP 333

[Close]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.4e-104
Identity = 184/328 (56.10%), Postives = 253/328 (77.13%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MV+LS+PT +  QY+ +R+    T S + IP +DLS P+AK+ I+KACE+FGFFK++NH 
Sbjct: 1   MVLLSKPTSE--QYTYVRNNMPITFS-SSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           +P++ +++LE E+  FF L ++EK+KA P +P GYG K IG NGD GWIEYLLL  N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 ILSQ-----NSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFR 180
             S      + F    ++Y  A++ ++ E+++L+AEGLK++P+N  SKL+ D+++DC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGAL 240
           VNHYP CPE+ ++ G N+IGFGEHTDPQIIS+LRSNN+SG QI LRDG+W+SVPPD  + 
Sbjct: 181 VNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSF 240

Query: 241 FVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESL 300
           F+NVGD+LQVMTNGRFKSV+H+V+A+    R+SMIYF GPPLSEKI PLP L+ +G+ESL
Sbjct: 241 FINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKESL 300

Query: 301 YKEFTWWEYKTAAYKTKLADYRLGAFEK 324
           YKEFTW+EYK++ Y ++LAD RLG +E+
Sbjct: 301 YKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.8e-99
Identity = 182/336 (54.17%), Postives = 237/336 (70.54%), Query Frame = 0


Query: 1   MVVLSQP-TLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNH 60
           MV++ QP + D N Y  +   CKP      IP IDL+D +AK+ I+KACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPL 120
            V  +L+T+LE E++ FF L  S KDKA PP P GYG K IG NGD GW+EY+LLNAN L
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN-L 120

Query: 121 PILSQNS----------FLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEK 180
            + S  +          F  A EEY++ +K++SS+ +E++ E LK+EP+  +S+L+  ++
Sbjct: 121 CLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKE 180

Query: 181 ADCCFRVNHYPPCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVP 240
           +D C R+NHY   PE +    +  IGFGEHTDPQ+IS+LRSN++ GLQIC++DG WV V 
Sbjct: 181 SDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVT 240

Query: 241 PDAGALFVNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLE 300
           PD  + FV VGD LQVMTNGRFKSVKH+VV +  R R+SMIYF GPPLSEKI PL  L+ 
Sbjct: 241 PDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAYKTKLADYRLGAFEKSP 326
           + ++ LY EFTW +YK +AYKTKL DYRLG FEK P
Sbjct: 301 KQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEKRP 328

[Close]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-95
Identity = 168/312 (53.85%), Postives = 228/312 (73.08%), Query Frame = 0


Query: 23  PTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSES 82
           P + F+ IP ID+SDP +K  ++KACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNAN---------PLPILSQNSFLCAAEE 142
           EK +    +P GYG   IG NGD GW+EYLL+NAN         P  + S  +F  A EE
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYPPCP--EMQSLGGR 202
           Y  +V+K++ +V+E I +GL ++PRN +SKL++D+  D   R+NHYPPCP    ++ GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIISVLRSNN+SGLQI L DG+W+SVPPD  + F NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFTWWEYKTAAYKT 322
           KSV+H+V+A+  + RVSMIYF GP L+++I PL  L++  +E LY+EFTW EYK + Y +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADYRLGAFEK 324
           +L+D RL  FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of Bhi03G000076 vs. NCBI nr
Match: XP_038883200.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 7.2e-191
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180
           ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP
Sbjct: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180

Query: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240
           PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG
Sbjct: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240

Query: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300
           DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT
Sbjct: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300

Query: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 328
           WWEYKTAAYKTKLADYRLGAFEKSPNS
Sbjct: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 327

[Close]

BLAST of Bhi03G000076 vs. NCBI nr
Match: XP_008439633.1 (PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.3e-176
Identity = 303/327 (92.66%), Postives = 312/327 (95.41%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLSESEKDKARPPHPLGYG KNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180
           I S + FLCAA EYV AVKKLS EVVELIAEGLKME RN ISKLLNDEKADCCFRVNHYP
Sbjct: 121 IFS-HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNHYP 180

Query: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240
           PCPEMQ L GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPD GALFVNVG
Sbjct: 181 PCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVNVG 240

Query: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300
           DALQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP+LLE+GEESLYKEFT
Sbjct: 241 DALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKEFT 300

Query: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 328
           WWEYKTAAYK+KLADYRLGAFEKSP S
Sbjct: 301 WWEYKTAAYKSKLADYRLGAFEKSPIS 326

[Close]

BLAST of Bhi03G000076 vs. NCBI nr
Match: KAA0052541.1 (gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 624.4 bits (1609), Expect = 5.6e-175
Identity = 302/327 (92.35%), Postives = 311/327 (95.11%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLSESEKDKARPPHPLGYG KNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180
           I S + FL AA EYV AVKKLS EVVELIAEGLKME RN ISKLLNDEKADCCFRVNHYP
Sbjct: 121 IFS-HDFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNHYP 180

Query: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240
           PCPEMQ L GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPD GALFVNVG
Sbjct: 181 PCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVNVG 240

Query: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300
           DALQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP+LLE+GEESLYKEFT
Sbjct: 241 DALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKEFT 300

Query: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 328
           WWEYKTAAYK+KLADYRLGAFEKSP S
Sbjct: 301 WWEYKTAAYKSKLADYRLGAFEKSPIS 326

[Close]

BLAST of Bhi03G000076 vs. NCBI nr
Match: NP_001295796.1 (gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >CCG14218.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 597.8 bits (1540), Expect = 5.6e-167
Identity = 292/327 (89.30%), Postives = 303/327 (92.66%), Query Frame = 0


Query: 1   MVVLSQ-PTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNH 60
           MVVLSQ PTLD NQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPL 120
            VPVELMTKLEDESLCFFKLS+SEKDKAR PHPLGYG KNIGSNGDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGSKNIGSNGDKGWIEYLLLNANPL 120

Query: 121 PILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHY 180
           PI S + FLCAA EYV AVKKLS EVVELIAEGLKME RN ISKLL DEKADCCFRVNHY
Sbjct: 121 PIFS-HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVNHY 180

Query: 181 PPCPEMQSLGG-RNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVN 240
           PPCPEMQ L G  NMIGFGEHTDPQI+S+LRSNNS+GLQICLRDGAWVSVP DA A FVN
Sbjct: 181 PPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKE 300
           VGD LQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP++L++GEESLYKE
Sbjct: 241 VGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADYRLGAFEKSP 326
           FTWWEYKTAAYK+KLADYRLGAFEKSP
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSP 326

[Close]

BLAST of Bhi03G000076 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 595.5 bits (1534), Expect = 2.8e-166
Identity = 284/331 (85.80%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+SHI++ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLE+E+LCFFKLS+SEKDKARPP PLGYGCKNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 ILSQN--SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180
           I SQN  SFL AA+EYV AVKKLS EV ELIAEGLK+EPRN ISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEMQSLG--GRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALF 240
           YPPCP++Q+LG  GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPDA A+F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVAD NRERVSMIYFGGPPLSEKI PLP++L +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADYRLGAFEKSPNS 328
           KEFTWWEYKTAAYKT+LADYRLG FEK+ NS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL
Match: A0A1S3AZ81 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 303/327 (92.66%), Postives = 312/327 (95.41%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLSESEKDKARPPHPLGYG KNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180
           I S + FLCAA EYV AVKKLS EVVELIAEGLKME RN ISKLLNDEKADCCFRVNHYP
Sbjct: 121 IFS-HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNHYP 180

Query: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240
           PCPEMQ L GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPD GALFVNVG
Sbjct: 181 PCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVNVG 240

Query: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300
           DALQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP+LLE+GEESLYKEFT
Sbjct: 241 DALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKEFT 300

Query: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 328
           WWEYKTAAYK+KLADYRLGAFEKSP S
Sbjct: 301 WWEYKTAAYKSKLADYRLGAFEKSPIS 326

[Close]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 2.7e-175
Identity = 302/327 (92.35%), Postives = 311/327 (95.11%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLSESEKDKARPPHPLGYG KNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 ILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHYP 180
           I S + FL AA EYV AVKKLS EVVELIAEGLKME RN ISKLLNDEKADCCFRVNHYP
Sbjct: 121 IFS-HDFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNHYP 180

Query: 181 PCPEMQSLGGRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVNVG 240
           PCPEMQ L GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPD GALFVNVG
Sbjct: 181 PCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVNVG 240

Query: 241 DALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKEFT 300
           DALQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP+LLE+GEESLYKEFT
Sbjct: 241 DALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKEFT 300

Query: 301 WWEYKTAAYKTKLADYRLGAFEKSPNS 328
           WWEYKTAAYK+KLADYRLGAFEKSP S
Sbjct: 301 WWEYKTAAYKSKLADYRLGAFEKSPIS 326

[Close]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL
Match: S0BE28 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.7e-167
Identity = 292/327 (89.30%), Postives = 303/327 (92.66%), Query Frame = 0


Query: 1   MVVLSQ-PTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNH 60
           MVVLSQ PTLD NQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPL 120
            VPVELMTKLEDESLCFFKLS+SEKDKAR PHPLGYG KNIGSNGDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGSKNIGSNGDKGWIEYLLLNANPL 120

Query: 121 PILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHY 180
           PI S + FLCAA EYV AVKKLS EVVELIAEGLKME RN ISKLL DEKADCCFRVNHY
Sbjct: 121 PIFS-HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVNHY 180

Query: 181 PPCPEMQSLGG-RNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVN 240
           PPCPEMQ L G  NMIGFGEHTDPQI+S+LRSNNS+GLQICLRDGAWVSVP DA A FVN
Sbjct: 181 PPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKE 300
           VGD LQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP++L++GEESLYKE
Sbjct: 241 VGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADYRLGAFEKSP 326
           FTWWEYKTAAYK+KLADYRLGAFEKSP
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSP 326

[Close]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.3e-166
Identity = 284/331 (85.80%), Postives = 310/331 (93.66%), Query Frame = 0


Query: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNHR 60
           MVVLSQP L L+QYSLLR+TCKPTASF +IPEIDLSDPNA+SHI++ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLE+E+LCFFKLS+SEKDKARPP PLGYGCKNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 ILSQN--SFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNH 180
           I SQN  SFL AA+EYV AVKKLS EV ELIAEGLK+EPRN ISKLLNDEK+DCCFRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEMQSLG--GRNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALF 240
           YPPCP++Q+LG  GRNMIGFGEHTDPQIIS+LRSNNS+GLQICLRDGAWVSVPPDA A+F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVAD NRERVSMIYFGGPPLSEKI PLP++L +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAYKTKLADYRLGAFEKSPNS 328
           KEFTWWEYKTAAYKT+LADYRLG FEK+ NS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL
Match: A0A0A0KIK7 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G523390 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 288/327 (88.07%), Postives = 299/327 (91.44%), Query Frame = 0


Query: 1   MVVLSQ-PTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKSHIIKACEEFGFFKLVNH 60
           MVVLSQ PTLD NQYSLLRSTCKPTASFADIPEIDLSDPNAK HI+KACEEFGFFKLVNH
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNH 60

Query: 61  RVPVELMTKLEDESLCFFKLSESEKDKARPPHPLGYGCKNIGSNGDKGWIEYLLLNANPL 120
            VPVELMTKLEDESLCFFKLS+SEKDKAR PHPLGY     GSNGDKGWIEYLLLNANPL
Sbjct: 61  GVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGY-----GSNGDKGWIEYLLLNANPL 120

Query: 121 PILSQNSFLCAAEEYVRAVKKLSSEVVELIAEGLKMEPRNGISKLLNDEKADCCFRVNHY 180
           PI S + FLCAA EYV AVKKLS EVVELIAEGLKME RN ISKLL DEKADCCFRVNHY
Sbjct: 121 PIFS-HDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFRVNHY 180

Query: 181 PPCPEMQSLGG-RNMIGFGEHTDPQIISVLRSNNSSGLQICLRDGAWVSVPPDAGALFVN 240
           PPCPEMQ L G  NMIGFGEHTDPQI+S+LRSNNS+GLQICLRDGAWVSVP DA A FVN
Sbjct: 181 PPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADPNRERVSMIYFGGPPLSEKITPLPQLLEEGEESLYKE 300
           VGD LQVMTNGRFKSVKHKVV DPNRERVSMIYFGGPPLSEKITPLP++L++GEESLYKE
Sbjct: 241 VGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEESLYKE 300

Query: 301 FTWWEYKTAAYKTKLADYRLGAFEKSP 326
           FTWWEYKTAAYK+KLADYRLGAFEKSP
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSP 321

The following BLAST results are available for this feature:

BLAST of Bhi03G000076 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	1.5e-109	58.63	gibberellin 2-oxidase	[more]
AT2G34555.1	6.3e-100	54.17	gibberellin 2-oxidase 3	[more]
AT1G78440.1	1.1e-96	53.85	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G02400.1	2.2e-73	44.55	gibberellin 2-oxidase 6	[more]
AT1G30040.2	5.0e-73	54.69	gibberellin 2-oxidase	[more]

BLAST of Bhi03G000076 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	9.4e-117	63.06	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9XFR9	2.1e-108	58.63	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9SQ80	2.4e-104	56.10	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O64692	8.8e-99	54.17	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8LEA2	1.6e-95	53.85	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]

BLAST of Bhi03G000076 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883200.1	7.2e-191	100.00	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]
XP_008439633.1	2.3e-176	92.66	PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo]	[more]
KAA0052541.1	5.6e-175	92.35	gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa] >TYK13284.1 gibbe...	[more]
NP_001295796.1	5.6e-167	89.30	gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >CCG14218.1 GA 2-oxidase [...	[more]
AUB30295.1	2.8e-166	85.80	gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca]	[more]

BLAST of Bhi03G000076 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AZ81	1.1e-176	92.66	gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3...	[more]
A0A5A7UGB1	2.7e-175	92.35	Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
S0BE28	2.7e-167	89.30	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1	[more]
A0A2H4V3T7	1.3e-166	85.80	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A0A0KIK7	5.3e-163	88.07	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 131..151
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..324
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..324
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 21..315
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..115 e-value: 7.0E-13 score: 49.4
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..315 e-value: 1.8E-78 score: 266.2
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..273 e-value: 1.4E-26 score: 92.9
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 169..275 score: 12.317982

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000076	Bhi03M000076	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding