Bhi03G000013 (gene) Wax gourd (B227) v1

BLAST of Bhi03G000013 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 878.2 bits (2268), Expect = 4.8e-255
Identity = 460/794 (57.93%), Postives = 564/794 (71.03%), Query Frame = 0

Query: 9   FVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKESEEDAKSS 68
           F LL L+P   L AS +   + YIVYFGEH GDK++ EIE  HHSYL SVKESEEDA++S
Sbjct: 8   FPLLFLIP---LLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARAS 67

Query: 69  LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIES--KKYRMHTTRSWEFSGVEEDKPT 128
           LLY+YKHSIN FAA LTP QASKL +L EVVSV +S  +KY  HTTRSWEF G+EE++  
Sbjct: 68  LLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETD 127

Query: 129 IN----------------DLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKG 188
            +                + + +A +G  +++G+LDSGVWP+SKSF+DKGMGP+PKSWKG
Sbjct: 128 SDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKG 187

Query: 189 ICQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETA--DYRSPCDKDGHGSHTASIAG 248
           ICQTG AF S+HCNRKIIGARYY+KGYE ++G  N TA  D+ SP D DGHGSHTAS A 
Sbjct: 188 ICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAV 247

Query: 249 GRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAI 308
           GRRV   SA GG A G+ASGGAP ARLAIYK CWA PN  K  GN+C + DMLAA+DDAI
Sbjct: 248 GRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAI 307

Query: 309 ADGVDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWII 368
           ADGV V+S+SIG ++P+ +T DGIA+GALHAVKR+IVV+ SAGN GP P  LSN+APWII
Sbjct: 308 ADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWII 367

Query: 369 TVGASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLC 428
           TVGAST+DR F   ++LGNG  IK  S+  +  K  K  PLVYA +++ P +  N++  C
Sbjct: 368 TVGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVPGIALNETSQC 427

Query: 429 VAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVP 488
           +  SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G    +D HFVP
Sbjct: 428 LPNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVP 487

Query: 489 ATAVSYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPD 548
              V+    + IL+YIK+  NP A I P  T+Y  + AP+M  FSSRGPN VDP+ LKPD
Sbjct: 488 TAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPD 547

Query: 549 ITAPGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWS 608
           ITAPG+ ILAAWS  DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS
Sbjct: 548 ITAPGLYILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 607

Query: 609 QAAIRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYT 668
            AAIRSALMTTA   N    P+ D       PA+PF+ GSGHFRP+KAADPGLVYD++Y 
Sbjct: 608 SAAIRSALMTTAWMTNDKKKPIQDTT---GLPANPFALGSGHFRPTKAADPGLVYDASYR 667

Query: 669 DYLHYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVG-GGGKS 728
            YL Y C + + +IDP+F CP +    ++ NYPSIAVP L+  V +KRTVTNVG G   S
Sbjct: 668 AYLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTS 727

Query: 729 VYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNG-YSFGWFAW 781
            Y F  + P G++V A PNIL FNR+G+++RF I +   +KN   N    G Y FGWF+W
Sbjct: 728 TYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIK-PLKNQVMNATEKGQYQFGWFSW 787

BLAST of Bhi03G000013 vs. TAIR 10
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-200
Identity = 384/734 (52.32%), Postives = 485/734 (66.08%), Query Frame = 0

Query: 76  SINAFAAILTPQQASKLSELDEVVSVIES--KKYRMHTTRSWEFSGVEED---------- 135
           SIN FAA LTP QAS+L EL EVVSV +S  +KY++HTTRSWEF G++E+          
Sbjct: 37  SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGEDYRSDGD 96

Query: 136 ----KPTIND-------LVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 195
               K  +ND        +  A +G  V++G++DSGVWP+S+SF DKGMGPIP+SWKGIC
Sbjct: 97  APRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPESWKGIC 156

Query: 196 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETA--DYRSPCDKDGHGSHTASIAGGR 255
           QTG AF S+HCN      RYY +GYE ++G  N  A  D+ SP D DGHGSHTAS A GR
Sbjct: 157 QTGVAFNSSHCN------RYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTASTAVGR 216

Query: 256 RVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIAD 315
           RV  VSA GG+A GTASGGA  ARLA+YK CWA+PN+ K   N CFD DMLAA DDAIAD
Sbjct: 217 RVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAFDDAIAD 276

Query: 316 GVDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITV 375
           GV+V+S+SIG  +P+ Y +DGIAIGALHAVKRDIVV+ SAGN GP    LSN APWIITV
Sbjct: 277 GVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPAPWIITV 336

Query: 376 GASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVA 435
           GAS++DR F   + LG+G   +  S+  + LK     PLVYA D++ P V RN + LC+ 
Sbjct: 337 GASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVVVPGVSRNDAMLCLP 396

Query: 436 GSLSHEKAKGKIVLCFRGEGISRFAG-SLEVKRSGGAGMILGNVPAVGRRPHADPHFVPA 495
            +LS +  +GK+VLC RG G     G  LEVKR+GG GMIL N          + HFVP 
Sbjct: 397 NALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVESHFVPT 456

Query: 496 TAVSYEDANMILKYIKSRTNPTANIVPPITI-YGSRPAPAMANFSSRGPNPVDPHFLKPD 555
             V     + IL YI +   P A I P  T+ Y ++P  ++  +    P P    FL PD
Sbjct: 457 ALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPYK---PAPFMTSFL-PD 516

Query: 556 ITAPGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWS 615
           I APG++ILAAWS  DS +K    +D R++ YNL SGTSMSCPHV+ A ALL+++HP+WS
Sbjct: 517 IIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIALLKSMHPTWS 576

Query: 616 QAAIRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYT 675
            AAIRSALMTTA+  N+   P+ D    D SPA+PF+ GS HFRP+KAA PGLVYD++Y 
Sbjct: 577 SAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPTKAASPGLVYDASYQ 636

Query: 676 DYLHYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVG--GGGK 735
            YL Y C + + ++DP+F CP R    ++LNYPSI++P L G V + RTVT VG  G   
Sbjct: 637 SYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTVTCVGRTGNST 696

Query: 736 SVYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAW 781
           SVY F+++ P GV V A PN+L F+++G++KRF I  + +          + Y FGWF+W
Sbjct: 697 SVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEARRDRYRFGWFSW 752

BLAST of Bhi03G000013 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 613.2 bits (1580), Expect = 2.9e-175
Identity = 359/793 (45.27%), Postives = 480/793 (60.53%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQ-ASSSHNHKAYIVYFGEHH-----GDKSWEEIEARHHSY 60
           M+ +  F F+LLLLL  +  +   +S +  +Y+VYFG H       + + + ++  H+ +
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRS 120
           L S   S E A  ++ Y+Y   IN FAA L    A ++S+  EVVSV  +K  ++HTTRS
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WEFSGVEEDKPTINDLVYR-ANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQ 180
           W+F G+E +    +  ++R A +G+D +I  LD+GVWP+SKSF D+G+GPIP  WKGICQ
Sbjct: 121 WDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ 180

Query: 181 TGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVY 240
                 + HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V 
Sbjct: 181 -NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVP 240

Query: 241 NVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVD 300
            VS F G   GTA GG+P AR+A YKVCW         GN C+D D+LAA D AI DG D
Sbjct: 241 GVSIF-GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGAD 300

Query: 301 VLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGAS 360
           V+S+S+G  +P ++ +D +AIG+ HA K+ IVV CSAGN GP  S +SN+APW ITVGAS
Sbjct: 301 VISVSLG-GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAS 360

Query: 361 TVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSL 420
           T+DREF S ++LGNG   KG S++ + L   K YP++ + +    +     + LC  GSL
Sbjct: 361 TMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSL 420

Query: 421 SHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVS 480
              K KGKI++C RG+   R      V   GG GM+L N    G    ADPH +PAT ++
Sbjct: 421 DPIKTKGKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLT 480

Query: 481 YEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPG 540
            +D+  + +YI     P A+I P  T  G +PAP MA+FSS+GP+ V P  LKPDITAPG
Sbjct: 481 SKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPG 540

Query: 541 VDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIR 600
           V ++AA++   SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +PSWS AAIR
Sbjct: 541 VSVIAAYTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIR 600

Query: 601 SALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHY 660
           SA+MTTAT ++    P+    +  N  A+PFSFG+GH +P+ A +PGLVYD    DYL++
Sbjct: 601 SAIMTTATIMDDIPGPI---QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF 660

Query: 661 LCGLKMNSIDPS------FTCPPRALHPHDLNYPSIAVPRLRGA-VRIKRTVTNVGGGGK 720
           LC L  N+   S      FTC    +   +LNYPSI VP L  + V + RTV NV  G  
Sbjct: 661 LCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV--GRP 720

Query: 721 SVYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAW 780
           S+Y      P GV V+  P  L F +VGE+K F + +   VK+  N     GY FG   W
Sbjct: 721 SMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVIL---VKSKGN--VAKGYVFGELVW 770

BLAST of Bhi03G000013 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 599.0 bits (1543), Expect = 5.6e-171
Identity = 357/782 (45.65%), Postives = 478/782 (61.13%), Query Frame = 0

Query: 9   FVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKESEEDAKSS 68
           F LLL L F  + +SSS +   YIV+  +     S++     + S L S+ +S E     
Sbjct: 11  FFLLLCLGFCHVSSSSS-DQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE----- 70

Query: 69  LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGVEEDKPTIN 128
           LLY Y+++I+ F+  LT ++A  L     V+SV+   +Y +HTTR+  F G++E      
Sbjct: 71  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 130

Query: 129 DLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQSAHCNRKII 188
           DL   A    DVV+G+LD+GVWP+SKS+SD+G GPIP SWKG C+ G  F ++ CNRK+I
Sbjct: 131 DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 190

Query: 189 GARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASG 248
           GAR++ +GYE   G ++E+ + RSP D DGHG+HT+S A G  V   S   G A GTA G
Sbjct: 191 GARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARG 250

Query: 249 GAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSDPYNYT 308
            AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VLS+S+G     +Y 
Sbjct: 251 MAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYY 310

Query: 309 DDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFYSPVILGNG 368
            DG+AIGA  A++R I+VSCSAGN GP+ S+LSN+APWI TVGA T+DR+F +  ILGNG
Sbjct: 311 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 370

Query: 369 LKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKGKIVLCFRG 428
               G+S+   +    K+ P +YAG+            LC+ G+L  EK KGKIV+C RG
Sbjct: 371 KNFTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRG 430

Query: 429 EGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMILKYIKSRT 488
                  G + VK +GG GMIL N  A G    AD H +PAT V  +  ++I  Y+ +  
Sbjct: 431 INARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP 490

Query: 489 NPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAWSEQDSPTK 548
           NPTA+I    T+ G +P+P +A FSSRGPN + P+ LKPD+ APGV+ILAAW+    PT 
Sbjct: 491 NPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTG 550

Query: 549 LPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATTINKSGH 608
           L    D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSALMTTA    K G 
Sbjct: 551 LAS--DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 610

Query: 609 PLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMNSID----- 668
           PL D A+    P++PF  G+GH  P+ A +PGL+YD    DYL +LC L   S       
Sbjct: 611 PLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 670

Query: 669 -PSFTC-PPRALHPHDLNYPSIAV-PRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA 728
             ++TC P ++    DLNYPS AV     GA +  RTVT+VGG G       SE   GV 
Sbjct: 671 RRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET-TGVK 730

Query: 729 VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS 783
           +S  P +L F    E+K +T+T +  V +S  + +N   SFG   W+DG H V SP+A+S
Sbjct: 731 ISVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 757

BLAST of Bhi03G000013 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 592.4 bits (1526), Expect = 5.2e-169
Identity = 347/789 (43.98%), Postives = 460/789 (58.30%), Query Frame = 0

Query: 4   SSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHH-----GDKSWEEIEARHHSYLMSV 63
           SS    V L   P   L+       K+YIVY G H           + +   H ++L S 
Sbjct: 22  SSLLLLVTLFFSPAFALK-------KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASF 81

Query: 64  KESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFS 123
             S E+AK ++ Y+YK  IN FAAIL   +A+++++  +VVSV  +K  ++HTT SW F 
Sbjct: 82  VGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 141

Query: 124 GVEEDKPTINDLVY-RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPA 183
            + ++       ++ +A YG+D +I  LD+GVWP+SKSFSD+G G +P  WKG C     
Sbjct: 142 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH---- 201

Query: 184 FQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSA 243
            +   CNRK+IGARY+ KGY  + G L   A Y +  D DGHGSHT S A G  V   + 
Sbjct: 202 -KDVPCNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 261

Query: 244 FGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSL 303
           F G+  GTASGG+P AR+A YKVCW   +     G  CFD D+LAA++ AI DGVDVLS 
Sbjct: 262 F-GIGNGTASGGSPKARVAAYKVCWPPVD-----GAECFDADILAAIEAAIEDGVDVLSA 321

Query: 304 SIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDR 363
           S+G  D  +Y  DGIAIG+ HAVK  + V CSAGN GP    +SN+APW+ITVGAS++DR
Sbjct: 322 SVG-GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDR 381

Query: 364 EFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEK 423
           EF + V L NG   KG S++   L  +KMY L+ A D    +     + LC  GSL  +K
Sbjct: 382 EFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKK 441

Query: 424 AKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDA 483
            KGKI++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y+D 
Sbjct: 442 VKGKILVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDG 501

Query: 484 NMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDIL 543
             +  Y+ S  +P   I  P     ++PAP MA+FSSRGPN + P  LKPDITAPGV+I+
Sbjct: 502 ETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNII 561

Query: 544 AAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALM 603
           AA++E   PT L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+M
Sbjct: 562 AAFTEATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIM 621

Query: 604 TTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGL 663
           TT+ T N    P+ D++      A+PFS+GSGH +P+KAA PGLVYD    DYL +LC +
Sbjct: 622 TTSRTRNNRRKPMVDESF---KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAV 681

Query: 664 KMNSI-------DPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYF 723
             N+        DP +TC   A +  D NYPSI VP L G++ + R + NV  G  + Y 
Sbjct: 682 GYNNTVVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNV--GPPATYN 741

Query: 724 FHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGI 780
                P GV VS  P  L FN+ GE K F +T+             +GY FG   WTD  
Sbjct: 742 ARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTP------SGYVFGELTWTDSH 774

BLAST of Bhi03G000013 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 6.7e-254
Identity = 460/794 (57.93%), Postives = 564/794 (71.03%), Query Frame = 0

Query: 9   FVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKESEEDAKSS 68
           F LL L+P   L AS +   + YIVYFGEH GDK++ EIE  HHSYL SVKESEEDA++S
Sbjct: 8   FPLLFLIP---LLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARAS 67

Query: 69  LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIES--KKYRMHTTRSWEFSGVEEDKPT 128
           LLY+YKHSIN FAA LTP QASKL +L EVVSV +S  +KY  HTTRSWEF G+EE++  
Sbjct: 68  LLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETD 127

Query: 129 IN----------------DLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKG 188
            +                + + +A +G  +++G+LDSGVWP+SKSF+DKGMGP+PKSWKG
Sbjct: 128 SDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKG 187

Query: 189 ICQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETA--DYRSPCDKDGHGSHTASIAG 248
           ICQTG AF S+HCNRKIIGARYY+KGYE ++G  N TA  D+ SP D DGHGSHTAS A 
Sbjct: 188 ICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAV 247

Query: 249 GRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAI 308
           GRRV   SA GG A G+ASGGAP ARLAIYK CWA PN  K  GN+C + DMLAA+DDAI
Sbjct: 248 GRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAI 307

Query: 309 ADGVDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWII 368
           ADGV V+S+SIG ++P+ +T DGIA+GALHAVKR+IVV+ SAGN GP P  LSN+APWII
Sbjct: 308 ADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWII 367

Query: 369 TVGASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLC 428
           TVGAST+DR F   ++LGNG  IK  S+  +  K  K  PLVYA +++ P +  N++  C
Sbjct: 368 TVGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVPGIALNETSQC 427

Query: 429 VAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVP 488
           +  SL  E   GK+VLC RG G SR    +EVKR+GGAGMILGN+ A G    +D HFVP
Sbjct: 428 LPNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVP 487

Query: 489 ATAVSYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPD 548
              V+    + IL+YIK+  NP A I P  T+Y  + AP+M  FSSRGPN VDP+ LKPD
Sbjct: 488 TAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPD 547

Query: 549 ITAPGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWS 608
           ITAPG+ ILAAWS  DSP+K+   +D R+  YN+YSGTSMSCPHV+ A ALL+AIHP WS
Sbjct: 548 ITAPGLYILAAWSGADSPSKMS--VDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWS 607

Query: 609 QAAIRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYT 668
            AAIRSALMTTA   N    P+ D       PA+PF+ GSGHFRP+KAADPGLVYD++Y 
Sbjct: 608 SAAIRSALMTTAWMTNDKKKPIQDTT---GLPANPFALGSGHFRPTKAADPGLVYDASYR 667

Query: 669 DYLHYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVG-GGGKS 728
            YL Y C + + +IDP+F CP +    ++ NYPSIAVP L+  V +KRTVTNVG G   S
Sbjct: 668 AYLLYGCSVNITNIDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTS 727

Query: 729 VYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNG-YSFGWFAW 781
            Y F  + P G++V A PNIL FNR+G+++RF I +   +KN   N    G Y FGWF+W
Sbjct: 728 TYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIK-PLKNQVMNATEKGQYQFGWFSW 787

BLAST of Bhi03G000013 vs. ExPASy Swiss-Prot
Match: F4KEL0 (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 806.2 bits (2081), Expect = 3.2e-232
Identity = 439/808 (54.33%), Postives = 547/808 (67.70%), Query Frame = 0

Query: 3   KSSFFCFVLLLLLPF-IQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKES 62
           K  F  F+ L LL F + L AS +   + YIVYFGEH GDK++ EIEA HHSYL SVKE+
Sbjct: 2   KRIFGIFIFLSLLLFLVPLLASCTKEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKET 61

Query: 63  EEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIES--KKYRMHTTRSWEFSG 122
           EEDA SSLLY    SIN FAA LTP QAS+L EL EVVSV +S  +KY++HTTRSWEF G
Sbjct: 62  EEDATSSLLYRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVG 121

Query: 123 VEED--------------KPTIND-------LVYRANYGKDVVIGMLDSGVWPKSKSFSD 182
           ++E+              K  +ND        +  A +G  V++G++DSGVWP+S+SF D
Sbjct: 122 LKEEEGEDYRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDD 181

Query: 183 KGMGPIPKSWKGICQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETA--DYRSPCDK 242
           KGMGPIP+SWKGICQTG AF S+HCNRKIIGARYY +GYE ++G  N  A  D+ SP D 
Sbjct: 182 KGMGPIPESWKGICQTGVAFNSSHCNRKIIGARYYARGYERYYGPFNAEANKDFLSPRDA 241

Query: 243 DGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCF 302
           DGHGSHTAS A GRRV  VSA GG+A GTASGGA  ARLA+YK CWA+PN+ K   N CF
Sbjct: 242 DGHGSHTASTAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCF 301

Query: 303 DTDMLAAMDDAIADGVDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPT 362
           D DMLAA DDAIADGV+V+S+SIG  +P+ Y +DGIAIGALHAVKRDIVV+ SAGN GP 
Sbjct: 302 DEDMLAAFDDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPA 361

Query: 363 PSALSNIAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDIL 422
              LSN APWIITVGAS++DR F   + LG+G   +  S+  + LK     PLVYA D++
Sbjct: 362 RETLSNPAPWIITVGASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVV 421

Query: 423 KPHVPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAG-SLEVKRSGGAGMILGNVPA 482
            P V RN + LC+  +LS +  +GK+VLC RG G     G  LEVKR+GG GMIL N   
Sbjct: 422 VPGVSRNDAMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SR 481

Query: 483 VGRRPHADPHFVPATAVSYEDANMILKYIKSRTNPTANIVPPITI-YGSRPAPAMANFSS 542
                  + HFVP   V     + IL YI +   P A I P  T+ Y ++P  ++  FSS
Sbjct: 482 DNDAFDVESHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPFSS 541

Query: 543 RGPNPVDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVS 602
           R PN VD + LKPDI APG++ILAAWS  DS +K    +D R++ YNL SGTSMSCPHV+
Sbjct: 542 RAPNWVDANILKPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVA 601

Query: 603 AAAALLRAIHPSWSQAAIRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPS 662
            A ALL+++HP+WS AAIRSALMTTA+  N+   P+ D    D SPA+PF+ GS HFRP+
Sbjct: 602 GAIALLKSMHPTWSSAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPT 661

Query: 663 KAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRI 722
           KAA PGLVYD++Y  YL Y C + + ++DP+F CP R    ++LNYPSI++P L G V +
Sbjct: 662 KAASPGLVYDASYQSYLLYCCSVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTV 721

Query: 723 KRTVTNVG--GGGKSVYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNN 781
            RTVT VG  G   SVY F+++ P GV V A PN+L F+++G++KRF I  + +      
Sbjct: 722 TRTVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTG 781

BLAST of Bhi03G000013 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 4.0e-174
Identity = 359/793 (45.27%), Postives = 480/793 (60.53%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQ-ASSSHNHKAYIVYFGEHH-----GDKSWEEIEARHHSY 60
           M+ +  F F+LLLLL  +  +   +S +  +Y+VYFG H       + + + ++  H+ +
Sbjct: 1   MKLTHNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDF 60

Query: 61  LMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRS 120
           L S   S E A  ++ Y+Y   IN FAA L    A ++S+  EVVSV  +K  ++HTTRS
Sbjct: 61  LGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRS 120

Query: 121 WEFSGVEEDKPTINDLVYR-ANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQ 180
           W+F G+E +    +  ++R A +G+D +I  LD+GVWP+SKSF D+G+GPIP  WKGICQ
Sbjct: 121 WDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ 180

Query: 181 TGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVY 240
                 + HCNRK+IGARY+ KGY    G LN + D  SP D DGHGSHT S A G  V 
Sbjct: 181 -NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVP 240

Query: 241 NVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVD 300
            VS F G   GTA GG+P AR+A YKVCW         GN C+D D+LAA D AI DG D
Sbjct: 241 GVSIF-GQGNGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGAD 300

Query: 301 VLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGAS 360
           V+S+S+G  +P ++ +D +AIG+ HA K+ IVV CSAGN GP  S +SN+APW ITVGAS
Sbjct: 301 VISVSLG-GEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAS 360

Query: 361 TVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSL 420
           T+DREF S ++LGNG   KG S++ + L   K YP++ + +    +     + LC  GSL
Sbjct: 361 TMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSL 420

Query: 421 SHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVS 480
              K KGKI++C RG+   R      V   GG GM+L N    G    ADPH +PAT ++
Sbjct: 421 DPIKTKGKILVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLT 480

Query: 481 YEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPG 540
            +D+  + +YI     P A+I P  T  G +PAP MA+FSS+GP+ V P  LKPDITAPG
Sbjct: 481 SKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPG 540

Query: 541 VDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIR 600
           V ++AA++   SPT   +  DPR + +N  SGTSMSCPH+S  A LL+  +PSWS AAIR
Sbjct: 541 VSVIAAYTGAVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIR 600

Query: 601 SALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHY 660
           SA+MTTAT ++    P+    +  N  A+PFSFG+GH +P+ A +PGLVYD    DYL++
Sbjct: 601 SAIMTTATIMDDIPGPI---QNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF 660

Query: 661 LCGLKMNSIDPS------FTCPPRALHPHDLNYPSIAVPRLRGA-VRIKRTVTNVGGGGK 720
           LC L  N+   S      FTC    +   +LNYPSI VP L  + V + RTV NV  G  
Sbjct: 661 LCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNV--GRP 720

Query: 721 SVYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAW 780
           S+Y      P GV V+  P  L F +VGE+K F + +   VK+  N     GY FG   W
Sbjct: 721 SMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVIL---VKSKGN--VAKGYVFGELVW 770

BLAST of Bhi03G000013 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 7.8e-170
Identity = 357/782 (45.65%), Postives = 478/782 (61.13%), Query Frame = 0

Query: 9   FVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKESEEDAKSS 68
           F LLL L F  + +SSS +   YIV+  +     S++     + S L S+ +S E     
Sbjct: 11  FFLLLCLGFCHVSSSSS-DQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE----- 70

Query: 69  LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGVEEDKPTIN 128
           LLY Y+++I+ F+  LT ++A  L     V+SV+   +Y +HTTR+  F G++E      
Sbjct: 71  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 130

Query: 129 DLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQSAHCNRKII 188
           DL   A    DVV+G+LD+GVWP+SKS+SD+G GPIP SWKG C+ G  F ++ CNRK+I
Sbjct: 131 DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 190

Query: 189 GARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASG 248
           GAR++ +GYE   G ++E+ + RSP D DGHG+HT+S A G  V   S   G A GTA G
Sbjct: 191 GARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARG 250

Query: 249 GAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSDPYNYT 308
            AP AR+A+YKVCW        LG  CF +D+LAA+D AIAD V+VLS+S+G     +Y 
Sbjct: 251 MAPRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYY 310

Query: 309 DDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFYSPVILGNG 368
            DG+AIGA  A++R I+VSCSAGN GP+ S+LSN+APWI TVGA T+DR+F +  ILGNG
Sbjct: 311 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 370

Query: 369 LKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKGKIVLCFRG 428
               G+S+   +    K+ P +YAG+            LC+ G+L  EK KGKIV+C RG
Sbjct: 371 KNFTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRG 430

Query: 429 EGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMILKYIKSRT 488
                  G + VK +GG GMIL N  A G    AD H +PAT V  +  ++I  Y+ +  
Sbjct: 431 INARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDP 490

Query: 489 NPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAWSEQDSPTK 548
           NPTA+I    T+ G +P+P +A FSSRGPN + P+ LKPD+ APGV+ILAAW+    PT 
Sbjct: 491 NPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTG 550

Query: 549 LPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTATTINKSGH 608
           L    D R V++N+ SGTSMSCPHVS  AALL+++HP WS AAIRSALMTTA    K G 
Sbjct: 551 LAS--DSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 610

Query: 609 PLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMNSID----- 668
           PL D A+    P++PF  G+GH  P+ A +PGL+YD    DYL +LC L   S       
Sbjct: 611 PLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVS 670

Query: 669 -PSFTC-PPRALHPHDLNYPSIAV-PRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA 728
             ++TC P ++    DLNYPS AV     GA +  RTVT+VGG G       SE   GV 
Sbjct: 671 RRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET-TGVK 730

Query: 729 VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS 783
           +S  P +L F    E+K +T+T +  V +S  + +N   SFG   W+DG H V SP+A+S
Sbjct: 731 ISVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 757

BLAST of Bhi03G000013 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 7.3e-168
Identity = 347/789 (43.98%), Postives = 460/789 (58.30%), Query Frame = 0

Query: 4   SSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHH-----GDKSWEEIEARHHSYLMSV 63
           SS    V L   P   L+       K+YIVY G H           + +   H ++L S 
Sbjct: 22  SSLLLLVTLFFSPAFALK-------KSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASF 81

Query: 64  KESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFS 123
             S E+AK ++ Y+YK  IN FAAIL   +A+++++  +VVSV  +K  ++HTT SW F 
Sbjct: 82  VGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFM 141

Query: 124 GVEEDKPTINDLVY-RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPA 183
            + ++       ++ +A YG+D +I  LD+GVWP+SKSFSD+G G +P  WKG C     
Sbjct: 142 LLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH---- 201

Query: 184 FQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSA 243
            +   CNRK+IGARY+ KGY  + G L   A Y +  D DGHGSHT S A G  V   + 
Sbjct: 202 -KDVPCNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANV 261

Query: 244 FGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSL 303
           F G+  GTASGG+P AR+A YKVCW   +     G  CFD D+LAA++ AI DGVDVLS 
Sbjct: 262 F-GIGNGTASGGSPKARVAAYKVCWPPVD-----GAECFDADILAAIEAAIEDGVDVLSA 321

Query: 304 SIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDR 363
           S+G  D  +Y  DGIAIG+ HAVK  + V CSAGN GP    +SN+APW+ITVGAS++DR
Sbjct: 322 SVG-GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDR 381

Query: 364 EFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEK 423
           EF + V L NG   KG S++   L  +KMY L+ A D    +     + LC  GSL  +K
Sbjct: 382 EFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKK 441

Query: 424 AKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDA 483
            KGKI++C RG+  +R    ++   +G AGM+L N  A G    +D H +PA+ + Y+D 
Sbjct: 442 VKGKILVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDG 501

Query: 484 NMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDIL 543
             +  Y+ S  +P   I  P     ++PAP MA+FSSRGPN + P  LKPDITAPGV+I+
Sbjct: 502 ETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNII 561

Query: 544 AAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALM 603
           AA++E   PT L    D R   +N  SGTSMSCPH+S    LL+ +HP WS AAIRSA+M
Sbjct: 562 AAFTEATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIM 621

Query: 604 TTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGL 663
           TT+ T N    P+ D++      A+PFS+GSGH +P+KAA PGLVYD    DYL +LC +
Sbjct: 622 TTSRTRNNRRKPMVDESF---KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAV 681

Query: 664 KMNSI-------DPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYF 723
             N+        DP +TC   A +  D NYPSI VP L G++ + R + NV  G  + Y 
Sbjct: 682 GYNNTVVQLFAEDPQYTCRQGA-NLLDFNYPSITVPNLTGSITVTRKLKNV--GPPATYN 741

Query: 724 FHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGI 780
                P GV VS  P  L FN+ GE K F +T+             +GY FG   WTD  
Sbjct: 742 ARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTP------SGYVFGELTWTDSH 774

BLAST of Bhi03G000013 vs. NCBI nr
Match: XP_038882517.1 (subtilisin-like protease SBT5.6 [Benincasa hispida])

HSP 1 Score: 1594.3 bits (4127), Expect = 0.0e+00
Identity = 782/782 (100.00%), Postives = 782/782 (100.00%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKE 60
           MEKSSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKE
Sbjct: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKE 60

Query: 61  SEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGV 120
           SEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGV
Sbjct: 61  SEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGV 120

Query: 121 EEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 180
           EEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS
Sbjct: 121 EEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 180

Query: 181 AHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 240
           AHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG
Sbjct: 181 AHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 300
           VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG
Sbjct: 241 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 300

Query: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFY 360
           KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKG 420
           SPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKG
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKG 420

Query: 421 KIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMI 480
           KIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMI
Sbjct: 421 KIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMI 480

Query: 481 LKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAW 540
           LKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAW
Sbjct: 481 LKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAW 540

Query: 541 SEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           SEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA
Sbjct: 541 SEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600

Query: 601 TTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN 660
           TTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN
Sbjct: 601 TTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN 660

Query: 661 SIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA 720
           SIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA
Sbjct: 661 SIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA 720

Query: 721 VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS 780
           VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS
Sbjct: 721 VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS 780

Query: 781 ST 783
           ST
Sbjct: 781 ST 782

BLAST of Bhi03G000013 vs. NCBI nr
Match: XP_008439697.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 [Cucumis melo])

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 721/793 (90.92%), Postives = 750/793 (94.58%), Query Frame = 0

Query: 1   MEKSSFF--CFVLLLLL------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARH 60
           MEKSSF   C VLLLLL      PFIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RH
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHT 120
           HSYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRM T
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
           TRSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWP SKSFSDKGMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240
           CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
           VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVG 360
           VDVLSLSIGKS+PYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSN+APWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAG 420
           ASTVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLVYAGDI+ PH PRNQSGLCVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SLSHEKAKGK VLCFRGEGISRFAGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATA
Sbjct: 421 SLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITA 540
           VSYEDAN+ILKYIKSRTNPTA IVPP+TIYGSRPAPAMANFSSRGPNP+DPHFLKPDITA
Sbjct: 481 VSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAA 600
           PGVDILAAWSEQDSPTKL KYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
           IRSALMTT+TT NKSG P+TDD++PDNSPA+PFSFGSGHFRPSKAADPGLVYDSNYTDYL
Sbjct: 601 IRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660

Query: 661 HYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFF 720
           HYLCGLKMNSIDPSFTCPPR LHPHDLNYPSIAVP+LR  VRIKRTVTNVGGGGKSVYFF
Sbjct: 661 HYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFF 720

Query: 721 HSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNN---NDNNNGYSFGWFAWTD 780
            SEAPPGVAVSASPNILYFNRVGERK+FTIT+S KV +S++   +     YSFGWFAW+D
Sbjct: 721 KSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAWSD 780

Query: 781 GIHYVRSPIAVSS 782
            IHYVRSPIAVS+
Sbjct: 781 EIHYVRSPIAVST 793

BLAST of Bhi03G000013 vs. NCBI nr
Match: XP_004134922.1 (subtilisin-like protease SBT5.6 isoform X2 [Cucumis sativus] >KGN49345.1 hypothetical protein Csa_003109 [Cucumis sativus])

HSP 1 Score: 1451.0 bits (3755), Expect = 0.0e+00
Identity = 718/791 (90.77%), Postives = 748/791 (94.56%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLL-------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARHH 60
           MEKSSF C   LLLL        FIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTT 120
           SYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRM TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGNYGPTPSALSN+APWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLVYAGDI+ PH PRNQSGLCVAGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSHEKAKGKIVLCFRGEGISRFAGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAP 540
           SYEDAN+ILKYIKSR NPTA IVPP+TIYGSRPAPAMANFSSRGPNP+DPHFLKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAI 600
           GVDILAAWSEQDSPTKLPKYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTT+TT NK G P+TDD++ DNSPA+PFSFGSGHFRPSKAADPGLVYDSNYTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFH 720
           YLCGLKMNSIDPSF CPPRALHPHDLNYPSIAVP+LR  VRIKRTVTNVGGGGK+VYFF 
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSN-NNDNNNGYSFGWFAWTDGIH 780
           SEAP GVAVSASPNILYFNRVGERK+FTIT+S KV N+N ++     YSFGWFAW+DGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

Query: 781 YVRSPIAVSST 783
           YVRSPIAVSST
Sbjct: 781 YVRSPIAVSST 791

BLAST of Bhi03G000013 vs. NCBI nr
Match: XP_031743664.1 (subtilisin-like protease SBT5.6 isoform X1 [Cucumis sativus])

HSP 1 Score: 1438.3 bits (3722), Expect = 0.0e+00
Identity = 718/813 (88.31%), Postives = 748/813 (92.00%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLL-------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARHH 60
           MEKSSF C   LLLL        FIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTT 120
           SYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRM TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNR----------------------KIIGARYYLKGYEHHFGRLNETADY 240
           QTGPAFQSAHCNR                      KIIGARYYLKGYEHHFGRLN+TADY
Sbjct: 181 QTGPAFQSAHCNRPQQPIGCHHRRLLVACSSCYTEKIIGARYYLKGYEHHFGRLNKTADY 240

Query: 241 RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA 300
           RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA
Sbjct: 241 RSPCDKDGHGSHTASIAGGRRVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKA 300

Query: 301 LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSA 360
           LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSA
Sbjct: 301 LGNVCFDTDMLAAMDDAIADGVDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSA 360

Query: 361 GNYGPTPSALSNIAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLV 420
           GNYGPTPSALSN+APWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLV
Sbjct: 361 GNYGPTPSALSNVAPWIITVGASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLV 420

Query: 421 YAGDILKPHVPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMIL 480
           YAGDI+ PH PRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEV+RSGGAGMIL
Sbjct: 421 YAGDIMNPHAPRNQSGLCVAGSLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMIL 480

Query: 481 GNVPAVGRRPHADPHFVPATAVSYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMA 540
           GNVPAVGRRPHADPHFVPATAVSYEDAN+ILKYIKSR NPTA IVPP+TIYGSRPAPAMA
Sbjct: 481 GNVPAVGRRPHADPHFVPATAVSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMA 540

Query: 541 NFSSRGPNPVDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSC 600
           NFSSRGPNP+DPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIV+YNLYSGTSMSC
Sbjct: 541 NFSSRGPNPIDPHFLKPDITAPGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSC 600

Query: 601 PHVSAAAALLRAIHPSWSQAAIRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGH 660
           PHVSAAAALLRAIHP+WSQAAIRSALMTT+TT NK G P+TDD++ DNSPA+PFSFGSGH
Sbjct: 601 PHVSAAAALLRAIHPTWSQAAIRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGH 660

Query: 661 FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRG 720
           FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSF CPPRALHPHDLNYPSIAVP+LR 
Sbjct: 661 FRPSKAADPGLVYDSNYTDYLHYLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRN 720

Query: 721 AVRIKRTVTNVGGGGKSVYFFHSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNS 780
            VRIKRTVTNVGGGGK+VYFF SEAP GVAVSASPNILYFNRVGERK+FTIT+S KV N+
Sbjct: 721 VVRIKRTVTNVGGGGKNVYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNN 780

Query: 781 N-NNDNNNGYSFGWFAWTDGIHYVRSPIAVSST 783
           N ++     YSFGWFAW+DGIHYVRSPIAVSST
Sbjct: 781 NRSSKKGEDYSFGWFAWSDGIHYVRSPIAVSST 813

BLAST of Bhi03G000013 vs. NCBI nr
Match: XP_022926980.1 (subtilisin-like protease SBT5.6 [Cucurbita moschata])

HSP 1 Score: 1367.1 bits (3537), Expect = 0.0e+00
Identity = 673/783 (85.95%), Postives = 721/783 (92.08%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHK-AYIVYFGEHHGDKSWEEIEARHHSYLMSVK 60
           ME SS FC +LLLLL    L  SS    K AYIVYFGEH GDKSWEEIE RHHSYLMSVK
Sbjct: 1   MEISSLFCLLLLLLL----LPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVK 60

Query: 61  ESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSG 120
           ++E DAKSSLLY+YKH+IN FAA+LT QQASKLSEL+EVVSVIESKKY MHTTRSWEFSG
Sbjct: 61  DTENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSG 120

Query: 121 VEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQ 180
           VEEDKP ++DLV +A  GKDVVIGMLDSGVWPKSKSFSDKGMGP+PKSWKGICQTGPAF+
Sbjct: 121 VEEDKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFE 180

Query: 181 SAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFG 240
           S+HCNRKIIGARYY+KGYEHHFGRLNET D+RSPCD DGHGSHTASIAGGRRVYNVSAFG
Sbjct: 181 SSHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFG 240

Query: 241 GVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSI 300
           GVA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGVDVLSLSI
Sbjct: 241 GVARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSI 300

Query: 301 GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREF 360
           GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSN+APWIITVGASTVDREF
Sbjct: 301 GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREF 360

Query: 361 YSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAK 420
           YSPVILGNGLKIKGLSVAPSKL+R KMYPLVYA DI KPHVPRN+SG C+AGSLSH+KAK
Sbjct: 361 YSPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAK 420

Query: 421 GKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANM 480
           GKIVLC+RG+GISR+AGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN 
Sbjct: 421 GKIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANR 480

Query: 481 ILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAA 540
           ILKYIKS  NPTA IVPPITIYGSRPAPAMANF+SRGP+ VDPHFLKPDITAPGVDILAA
Sbjct: 481 ILKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAA 540

Query: 541 WSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 600
           WSE+DSPTKLPK  DPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTT
Sbjct: 541 WSEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTT 600

Query: 601 ATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKM 660
           ATT NK GHP+TDD++ DNSPA+PFSFGSGHFRP+KAADPGLVYD+NYTDYLHYLCGLKM
Sbjct: 601 ATTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM 660

Query: 661 NSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGV 720
           NSID SF CPPRALHPHDLNYPSIA+P+L+G VRIKRTVTNVGGGGKSVYFF S APPGV
Sbjct: 661 NSIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGV 720

Query: 721 AVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAV 780
           AV ASP++LYF+RVG+RKRFTITVSGKVK        NGYSFGWFAW+DG+HYVRSPIA+
Sbjct: 721 AVRASPSVLYFDRVGQRKRFTITVSGKVK-------GNGYSFGWFAWSDGVHYVRSPIAI 772

Query: 781 SST 783
           SST
Sbjct: 781 SST 772

BLAST of Bhi03G000013 vs. ExPASy TrEMBL
Match: A0A1S3B024 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=LOC103484415 PE=3 SV=1)

HSP 1 Score: 1453.7 bits (3762), Expect = 0.0e+00
Identity = 721/793 (90.92%), Postives = 750/793 (94.58%), Query Frame = 0

Query: 1   MEKSSFF--CFVLLLLL------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARH 60
           MEKSSF   C VLLLLL      PFIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RH
Sbjct: 1   MEKSSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKERH 60

Query: 61  HSYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHT 120
           HSYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRM T
Sbjct: 61  HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMET 120

Query: 121 TRSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
           TRSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWP SKSFSDKGMGP+PKSWKGI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKGI 180

Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240
           CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRR 240

Query: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
           VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300

Query: 301 VDVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVG 360
           VDVLSLSIGKS+PYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSN+APWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360

Query: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAG 420
           ASTVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLVYAGDI+ PH PRNQSGLCVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420

Query: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
           SLSHEKAKGK VLCFRGEGISRFAGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATA
Sbjct: 421 SLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480

Query: 481 VSYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITA 540
           VSYEDAN+ILKYIKSRTNPTA IVPP+TIYGSRPAPAMANFSSRGPNP+DPHFLKPDITA
Sbjct: 481 VSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540

Query: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAA 600
           PGVDILAAWSEQDSPTKL KYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600

Query: 601 IRSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
           IRSALMTT+TT NKSG P+TDD++PDNSPA+PFSFGSGHFRPSKAADPGLVYDSNYTDYL
Sbjct: 601 IRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660

Query: 661 HYLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFF 720
           HYLCGLKMNSIDPSFTCPPR LHPHDLNYPSIAVP+LR  VRIKRTVTNVGGGGKSVYFF
Sbjct: 661 HYLCGLKMNSIDPSFTCPPRPLHPHDLNYPSIAVPQLRDVVRIKRTVTNVGGGGKSVYFF 720

Query: 721 HSEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNN---NDNNNGYSFGWFAWTD 780
            SEAPPGVAVSASPNILYFNRVGERK+FTIT+S KV +S++   +     YSFGWFAW+D
Sbjct: 721 KSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGWFAWSD 780

Query: 781 GIHYVRSPIAVSS 782
            IHYVRSPIAVS+
Sbjct: 781 EIHYVRSPIAVST 793

BLAST of Bhi03G000013 vs. ExPASy TrEMBL
Match: A0A0A0KHZ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1)

HSP 1 Score: 1451.0 bits (3755), Expect = 0.0e+00
Identity = 718/791 (90.77%), Postives = 748/791 (94.56%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLL-------PFIQLQASSSHNH-KAYIVYFGEHHGDKSWEEIEARHH 60
           MEKSSF C   LLLL        FIQ  ASSS+N  KAYIVYFGEHHG+KS EEI+ RHH
Sbjct: 1   MEKSSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERHH 60

Query: 61  SYLMSVKESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTT 120
           SYLM VKESEEDAKS LLYNYKHSINAFAAILTPQQASKLS+LDEVVSVIESKKYRM TT
Sbjct: 61  SYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMETT 120

Query: 121 RSWEFSGVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180
           RSWEFSGVEEDKPTINDLV RANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC
Sbjct: 121 RSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGIC 180

Query: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRV 240
           QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLN+TADYRSPCDKDGHGSHTASIAGGRRV
Sbjct: 181 QTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRRV 240

Query: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300
           YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV
Sbjct: 241 YNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGV 300

Query: 301 DVLSLSIGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGA 360
           DVLSLSIGKS+PYNYTDDG+AIGALHAVK+DIVVSCSAGNYGPTPSALSN+APWIITVGA
Sbjct: 301 DVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVGA 360

Query: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGS 420
           STVDREFYSPVILGNGLKIKGLSVAPSKL+RKKMYPLVYAGDI+ PH PRNQSGLCVAGS
Sbjct: 361 STVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAGS 420

Query: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480
           LSHEKAKGKIVLCFRGEGISRFAGSLEV+RSGGAGMILGNVPAVGRRPHADPHFVPATAV
Sbjct: 421 LSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATAV 480

Query: 481 SYEDANMILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAP 540
           SYEDAN+ILKYIKSR NPTA IVPP+TIYGSRPAPAMANFSSRGPNP+DPHFLKPDITAP
Sbjct: 481 SYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITAP 540

Query: 541 GVDILAAWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAI 600
           GVDILAAWSEQDSPTKLPKYLDPRIV+YNLYSGTSMSCPHVSAAAALLRAIHP+WSQAAI
Sbjct: 541 GVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAAI 600

Query: 601 RSALMTTATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660
           RSALMTT+TT NK G P+TDD++ DNSPA+PFSFGSGHFRPSKAADPGLVYDSNYTDYLH
Sbjct: 601 RSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYLH 660

Query: 661 YLCGLKMNSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFH 720
           YLCGLKMNSIDPSF CPPRALHPHDLNYPSIAVP+LR  VRIKRTVTNVGGGGK+VYFF 
Sbjct: 661 YLCGLKMNSIDPSFKCPPRALHPHDLNYPSIAVPQLRNVVRIKRTVTNVGGGGKNVYFFK 720

Query: 721 SEAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSN-NNDNNNGYSFGWFAWTDGIH 780
           SEAP GVAVSASPNILYFNRVGERK+FTIT+S KV N+N ++     YSFGWFAW+DGIH
Sbjct: 721 SEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAWSDGIH 780

Query: 781 YVRSPIAVSST 783
           YVRSPIAVSST
Sbjct: 781 YVRSPIAVSST 791

BLAST of Bhi03G000013 vs. ExPASy TrEMBL
Match: A0A6J1EMK8 (subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE=3 SV=1)

HSP 1 Score: 1367.1 bits (3537), Expect = 0.0e+00
Identity = 673/783 (85.95%), Postives = 721/783 (92.08%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHK-AYIVYFGEHHGDKSWEEIEARHHSYLMSVK 60
           ME SS FC +LLLLL    L  SS    K AYIVYFGEH GDKSWEEIE RHHSYLMSVK
Sbjct: 1   MEISSLFCLLLLLLL----LPPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVK 60

Query: 61  ESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSG 120
           ++E DAKSSLLY+YKH+IN FAA+LT QQASKLSEL+EVVSVIESKKY MHTTRSWEFSG
Sbjct: 61  DTENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSG 120

Query: 121 VEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQ 180
           VEEDKP ++DLV +A  GKDVVIGMLDSGVWPKSKSFSDKGMGP+PKSWKGICQTGPAF+
Sbjct: 121 VEEDKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFE 180

Query: 181 SAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFG 240
           S+HCNRKIIGARYY+KGYEHHFGRLNET D+RSPCD DGHGSHTASIAGGRRVYNVSAFG
Sbjct: 181 SSHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFG 240

Query: 241 GVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSI 300
           GVA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGVDVLSLSI
Sbjct: 241 GVARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSI 300

Query: 301 GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREF 360
           GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSN+APWIITVGASTVDREF
Sbjct: 301 GKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREF 360

Query: 361 YSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAK 420
           YSPVILGNGLKIKGLSVAPSKL+R KMYPLVYA DI KPHVPRN+SG C+AGSLSH+KAK
Sbjct: 361 YSPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAK 420

Query: 421 GKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANM 480
           GKIVLC+RG+GISR+AGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN 
Sbjct: 421 GKIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANR 480

Query: 481 ILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAA 540
           ILKYIKS  NPTA IVPPITIYGSRPAPAMANF+SRGP+ VDPHFLKPDITAPGVDILAA
Sbjct: 481 ILKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAA 540

Query: 541 WSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTT 600
           WSE+DSPTKLPK  DPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTT
Sbjct: 541 WSEKDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTT 600

Query: 601 ATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKM 660
           ATT NK GHP+TDD++ DNSPA+PFSFGSGHFRP+KAADPGLVYD+NYTDYLHYLCGLKM
Sbjct: 601 ATTTNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM 660

Query: 661 NSIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGV 720
           NSID SF CPPRALHPHDLNYPSIA+P+L+G VRIKRTVTNVGGGGKSVYFF S APPGV
Sbjct: 661 NSIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGV 720

Query: 721 AVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAV 780
           AV ASP++LYF+RVG+RKRFTITVSGKVK        NGYSFGWFAW+DG+HYVRSPIA+
Sbjct: 721 AVRASPSVLYFDRVGQRKRFTITVSGKVK-------GNGYSFGWFAWSDGVHYVRSPIAI 772

Query: 781 SST 783
           SST
Sbjct: 781 SST 772

BLAST of Bhi03G000013 vs. ExPASy TrEMBL
Match: A0A6J1KU93 (subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3 SV=1)

HSP 1 Score: 1359.4 bits (3517), Expect = 0.0e+00
Identity = 664/782 (84.91%), Postives = 717/782 (91.69%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNHKAYIVYFGEHHGDKSWEEIEARHHSYLMSVKE 60
           ME S+ FC +LLLLLP     +S     +AYIVYFGEH GDKSWEEIE RHHSYLMSVK+
Sbjct: 1   METSTLFCLLLLLLLP----PSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKD 60

Query: 61  SEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFSGV 120
           +E DAKSSLLY+YKH+IN FAA+LT QQASKLSEL+EVVSVIESK Y MHTTRSWEFSGV
Sbjct: 61  TENDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTTRSWEFSGV 120

Query: 121 EEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAFQS 180
            EDKP ++DLV +A  GKDVVIGMLDSGVWPKSKSFSD GMGP+PKSWKGICQTGP F+S
Sbjct: 121 VEDKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGICQTGPGFES 180

Query: 181 AHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAFGG 240
            HCNRKIIGARYY+KGYEHHFGRLNET D+RSPCD DGHGSHTASIAGGRRVYNVSAFGG
Sbjct: 181 PHCNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGG 240

Query: 241 VAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLSIG 300
           VA GTASGGAP  RLAIYKVCWAIPNQMK+LGNVCFDTD+LAAMDDAI+DGVDVLSLSIG
Sbjct: 241 VARGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIG 300

Query: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDREFY 360
           KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGN GPTPS LSN+APWIITVGASTVDREFY
Sbjct: 301 KSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFY 360

Query: 361 SPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKAKG 420
           SPVILGNGLKIKGLSVAPSKL+R KMYPLVYA DI KPHVP+N+SG C+AGSLSH+KAKG
Sbjct: 361 SPVILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPQNESGSCIAGSLSHKKAKG 420

Query: 421 KIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDANMI 480
           KIVLC+RG+GISR+AGSLEVKRSGGAGMILG VPAVG R HADPHFVPATAVSY+DAN I
Sbjct: 421 KIVLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRI 480

Query: 481 LKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILAAW 540
           LKYIKS  NPTA IVPPITIYGSRPAPAMANF+SRGP+ VDPHFLKPDITAPGVDILAAW
Sbjct: 481 LKYIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAW 540

Query: 541 SEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMTTA 600
           SE+DSPT LPK+LDPRIVKYN+YSGTSMSCPHVSAAAALLRAIHP+WSQAAIRSALMTT+
Sbjct: 541 SEKDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTS 600

Query: 601 TTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLKMN 660
           TT NK GHP+TDD++ DNSPA+PFSFGSGHFRP+KAADPGLVYD+NYTDYLHYLCGLKMN
Sbjct: 601 TTTNKYGHPITDDSTSDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKMN 660

Query: 661 SIDPSFTCPPRALHPHDLNYPSIAVPRLRGAVRIKRTVTNVGGGGKSVYFFHSEAPPGVA 720
           SID SF CPPRALHPHDLNYPSIA+P+L+G VRIKRTVTNVGGGGKSVYFF S APPGVA
Sbjct: 661 SIDRSFKCPPRALHPHDLNYPSIAIPKLKGGVRIKRTVTNVGGGGKSVYFFESWAPPGVA 720

Query: 721 VSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVRSPIAVS 780
           V ASP++LYF+RVG+RKRFT+TVSGKVK S       GYSFGWFAW+DGIHYVRSPIA+S
Sbjct: 721 VRASPSVLYFDRVGQRKRFTVTVSGKVKGS-------GYSFGWFAWSDGIHYVRSPIAIS 771

Query: 781 ST 783
           ST
Sbjct: 781 ST 771

BLAST of Bhi03G000013 vs. ExPASy TrEMBL
Match: A0A6J1CJX5 (subtilisin-like protease SBT5.6 OS=Momordica charantia OX=3673 GN=LOC111012300 PE=3 SV=1)

HSP 1 Score: 1302.7 bits (3370), Expect = 0.0e+00
Identity = 648/788 (82.23%), Postives = 710/788 (90.10%), Query Frame = 0

Query: 1   MEKSSFFCFVLLLLLPFIQLQASSSHNH--KAYIVYFGEHHGDKSWEEIEARHHSYLMSV 60
           MEK S F  +LLLLL  +  QAS S N+   AYIVYFGEH GDK+W EIEA HHSYL SV
Sbjct: 32  MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 91

Query: 61  KESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFS 120
           K++EE+A+SSL+Y+YKHSIN FAA+L+ ++ASKLSE+D VV VI S+KY   TTRSWEFS
Sbjct: 92  KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFS 151

Query: 121 GVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAF 180
           GVEEDKP +NDLV RA +GKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSW+GICQTGPAF
Sbjct: 152 GVEEDKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAF 211

Query: 181 QSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAF 240
           QSAHCNRKIIGARYYLKGYE++FGRLNET DYRSPCDKDGHGSHTAS AGGRRV+NVSAF
Sbjct: 212 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 271

Query: 241 GGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLS 300
           GGVA GTASGGAP ARLAIYKVCWAIP QMK LGNVCFDTD+LAAMDDAIADGVDVLSLS
Sbjct: 272 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 331

Query: 301 IGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDRE 360
           IGK+DPYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSN APWIITVGASTVDRE
Sbjct: 332 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 391

Query: 361 FYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKA 420
           FYSPVILGNG KI+GLSVAPS LKRKKMYPLVYAGDI+KPHVP+NQSG+CVAGSLSHEKA
Sbjct: 392 FYSPVILGNGFKIRGLSVAPSMLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 451

Query: 421 KGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDAN 480
           +GKIVLC+R EGISR+AGSLEVKRSGGAGMILGNVPAVGR+ HADPHFVPATAVSYEDAN
Sbjct: 452 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 511

Query: 481 MILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILA 540
           +ILKYIKSRTNPTA IVPPITIYGSRPAPAMANFSSRGPNP+DPH LKPDITAPGVDILA
Sbjct: 512 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 571

Query: 541 AWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMT 600
           AWS +DSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHP+WSQAAIRSALMT
Sbjct: 572 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 631

Query: 601 TATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLK 660
           TATT NK G P+TDDAS  ++PA+PFS+GSGHFRPSKAADPGLVYD+NYTDYL +LC LK
Sbjct: 632 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 691

Query: 661 M-NSIDPSFTCPPRALHPHDLNYPSIAVPRLR----GAVRIKRTVTNVGGGGKSVYFFHS 720
           M +SIDPSFTCP RA   HDLNYPS+AVPRLR    G VR+ RTV NV GG KS YFF +
Sbjct: 692 MAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGGVRVVRTVRNV-GGSKSAYFFRA 751

Query: 721 EAPPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYV 780
            AP GV+V ASP++LYF+R+G+RKRFTIT+SGK +      + +GYSFGWFAWTDGIH+V
Sbjct: 752 SAPAGVSVRASPSVLYFSRMGQRKRFTITISGKAE--IGGVDGSGYSFGWFAWTDGIHFV 811

Query: 781 RSPIAVSS 782
           RSPIA+SS
Sbjct: 812 RSPIAISS 815