Bhi02G001764 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001764
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionvesicle-associated protein 2-2-like isoform X2
Locationchr2: 57583446 .. 57588891 (-)
RNA-Seq ExpressionBhi02G001764
SyntenyBhi02G001764
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAACAAGAAAGTCGATTGACATGCAGAACAAAGGAACTCTTAATAACATGTAACTCCGTCACTTCGCTCCTTCTTCTCGGTCCACAGCACAAACAGCTTTCCTTCTCTTTCCGAATCACTTCTATTCCTTTCTCCACTTTTCTCTGTGTTCCTTTCTGATTCCCTATTCCAATCCTTTACCCCTTTGGATTTATATCATGAAACTCCCTTCCTTGCTTTTTCTTTTTCAATCACAATATTCCATCACTTTCTGCTATTCACTTCACTCTGTTTTGTTCGTATACCGTTCGAAATGATGACCCAATTCATCAACTAATCCCCCACTTCTTTTCTTTCAACTTTTTGAAGCTGATCAGTTGAGTTCTGCTGACCGGCCAACTCATTGTGTTGTCGCCAATTTCTTGTTGATTGTAATTTGTGAGTTTTCTTATACATTAAACTGTGTAGAGCTGTATTTTTAATATTATATTGGTGATTGTTTATTGTAGAAATCTTTAGGGTGTTTTTTTAATTGTTGGGTTTGAATAATTTAATTGGGTTCTTTGTTTGTATATGGATGCATCATGTCTGGGTTTCTATGATTATGTTTGATTTGTGCCTTTTCTTTTATACACTAATCCCCAAAAGTCTCTGCTTTGATTTTTGTTGTTTTTTGTAATTCGATTGTGCAGGACAAGTGTTTGGTTTAATAGCTGAGTGAAAGTATGGATATGAGGCTTTTAGAAATTCATCCTCGCGAATTGAAGTTCATTTGTAAGTCTACAGAATGAATTATTCTCTCTCTTTAGACTTTTGTTTTGTTGTTTTCAATGGGCTGTTCATCGATCATCTCTGAAACCATTAGGAAATTAATTGCTGCTTTTGTTTCTTACTATGAAACGTTTGATCTTTTGTGGCAAAAGCAAAGTGGTTTTCTTGTTTGGGTGTTGTTTTAGTACCTTTTAGTTTTTGAAATTTATGTTTTAATTCTTTCGTTTGTACTTTGTATGCTAATTGATCAGAGCCGAAGGGGTAAAAAGTTCCTTTGAATAACTATAAACATTTTTCAAATTTTTGTTGGTATTTGGTTGTATGGATAGGACATGGTTTTTATAATTTTGAAGCTCTTAAAAGGTTCTATTTGGATAAGCGCTTAGATTTAGGTTTGAAATGTGTTTTGGACCTAATGCGTTTAGGTGGAACACATTTTTACAAGCAATTTTGATAAATGAACACTAATGAAGTGCTTACTTTTGAAGTATTATAAAAAAACTTTTCAGTTTTTTAAAAGTTTTAAAAGCACTTTTGACTTCATCAGTCGCTAGGAGTTTATAAAGCATTTTTAATTAGTTAAAAGTACTTTTTACTCCTCTAAAACTCACCTTTTGTGCTTTTCCTGAAAGTGCTTATTGAAACGTAAAAGCTATCCCATGCTGAAGTTTTGATGTATGATATTTTCTATGATGTCTGCTCTGGATTTAATTTGCTAAGCTACCTCAAATGGAGTGTCATATCTCCATTTTCTCATTCGCTACTAAGACATTGCTTACTTTGGATTTATTCATTTATTATTATTTTTTGTATGAACAAGGATATGCTATTTTCCACTTCATTTTATCATTCATTCCATTTTTCAAATTTAGAACTAATATCGTTCATATTTCCTTCTATTGGCTCCAGTTGAACTAAATAAACATAGCTTATGCTCAATTCAACTCACAAATAATACACTCGACCATGTTGCTTTTAAGGTATGGGTTGCTGATCTTCCTCTGTGTCTCTGTCTCATTTTTTTCTCTCTCTTGTACTTTTCAAAGTCAATCAAAGTGATTTATGATTCATTAGGTAGAGATAACATGTTAAACAGTATGCAAGATAATGGATATATATATATATATATTTTTTTTTTCCAGGTTAAGATCACATCCCCGAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGTGCCAAACTCAACTTGCGAATTCACGGGTATATACTTTTGACTAGTTAAGGCTTTATATTTACTCTCATTCTTGTTTCAGGAGGGGAAGAGGGAATGTATGGGTGCCATTTTTATTTCTAGAAGTTGATAAATTGAAGTCATGGAATCATGAGTGATCTTTTATCTTAAATAGCTTTAATGAGGCCTCATTTTGGCTACGTTCTTCTGCTTCTAGCATCTTTGCTTGATTAGTTTTAGCATCATTTTTTCTATGCTTTCATTTCATGGCGCAGTCTGTAAACCTTTTTTTCCTCCAACTTTAGGGCACAGCTTGTCTAGTTTGACCTGCGTAGATTTTTAGGCCAATAGTTATATTGGTCATTATGAAATGGAATTAGTTGTTCTTGATGAAATACTTGATGTGTTGATAATTTCTAACTGATTTTTTATCCCTCTCTCCTACTTTTTATTTATTTATTTATTATTTTTATTTATTTTAAATCTTATATTTATCTTGACATCACTGTGTGAGGCCTTCAATCAGGAAACGATATTCTAGAAAGGGCAGTAAGGGGAAATCACACTCTACAAGTTAGTTTTGTAACGCAGAATAGGAAAGGAAGAGGGGCCCAATAAGGATGTGGGCTATATATACGTCTGGGAGTGAGGAATGTTTCTTTCAATGCTTTTTGTGAATTTTCTCTTGGTTGCAAGAAAGGAGAGATTACCCACCTTCACTAATCAATTTTGTATCGCCTCAAAACTCAACTTTCCTCTGTTTATCAATATATAGTAAGGGAGAAACCTTACACAATGATTGGCTCAAGTGCTTAGATTTAGGTTTGAAATGTGTTTTGGACCTAACGCGTTTAGGTGGAACACATTTTTACAAGCAATTTTGATAAATGAACACTAATGAAGTGCTTACTTTTGAAGTATTATAAAAAAACTTTTCAGTTTTTTAAAAGTTTTAAAAGCACTTTTGACTTCATCAGTCGCTAGGAGTTTATAAAGCATTTTTAATTAGTTAAAAGTACTTTTTACTCCTCTAAAACTCACCTTTTATGCTTTTCACAGCTTGCACTAATCATGGGCTATCCTCTGTTTTGTCATATTGCTCTCATTATTTCATTACCCCTTTCTCTCTGTCTTTCCAAATTTTATATAATGGCTCTTAATGTAGTTAGGATGCAAGCACAAACGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGTGGACATAACCTCTACCTTGGTAAATGCTTCTACTAGAATTTATTATGTATAATTGTCTATTTTTTTTTGGAATTTAACGTTTGTTTCCTTAACTTTCAGTTTGCCAAAGAAGGTGGGAAGTACATCGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCATTTCTCCAGTGGTTTCCCCAGTTGATGAAGCATTAAAGGAGGAGGAAGTGGCTCTTGCAGTTACAGAACCAAATAATCTATGTAAAGCAGTGGAGGAGATTGTAGACCAGGAACCTTTAAACAAAAACCATGTAATGACTAATGGAGTTCACAGCCTGCCGCTAAAATACATTATTACAATTTCTCAAACATGCCCTTTTTCTATGCTTGCTTCATAAGACATTTACTTTTATCCCAAGTTGATTAGGATCCTTAGATAAGCAATCATGGTAGAGCGTTTTTGAGAATGCATTGGAGTGCATCGAAACTTGGCACATGACAATAGCTAAATGTTTTGTTGAACCTGTGATGAGATGCATATCTCTACCAATCTAGCTATAACCATTTTTCTTTGCTTATCAAATCACAAAATTTTGGTTGTCCTATGAATTGTACCAGGTTCCCGGCAATGCTACGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCATCATGTTTACCTAATGACTTCATAGATGGCAATGAGGAGAATCTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTGAGTGAACTCGAGTTGAAGCTTTGTCAGGTCAGTCTTCCATTAAATATTACTCCAATATTATGAACTCTGAGCATAAGGATGAAGTGGAAAGACGGTCCGAAAACCTTATTTTACTGTTAACTGAAAAAGTTCTGCTAAACGGTAATGTGAATTGATGAACTTTCTTTACAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGAAGGAGAATTTTGTAATTTCCTTTACAATTTTAATTTTTCCTTGAACATATAATTCTTATCACCATCACGAGACGGATGCCGTTTATTGTTGCCTACTTTATATCATGAAAATATATCTGAAGACAGTTTATGATTTCTGTTGGTCTGTTGAAGTCCAAATATAGTTTTTTCTGTCAACGTCCATAGATACCTACATGAACTTTCCTCCAGTTTTCTTCAATTGAAATGAGGTCAGGGGTTACAAATTTAATGAATGAGCTTTGATAGCGATATCTAGTAGGCCCCAAAACTTTAAACGGTTTAATTACCTTCTAAACTTTAAATTATGATTTTGTATAGTTACCTTAGTGTTAAATGATAATGCAACATTTAGTGGTAAATGTTGGCTGCTTTAGCAGAATATGGTAGGCAAAGGATTAAGGGAGTGGATAATAGGAGAAAATAAGTGTCATAGTTTATTACAGGGATTTGATGTTACATTCCTTTCAACTAGACAGTACACTAAGCATATTGCTTTATTTTTTCTAGGTGTACACTTTTGTCCCAGTCATTTAGTCAATGGTATTAATATAGAATAACAGTTTCAAAGTTCAGTTTAGATATATAAAACATTGAACTTTGAAAGTTTGAAATACTATAGTAAAATCTACTGCGTTTTGAGGACTGTTTGTTTCAGCAATGGCGAGAATTTGAAGCCGTTTTTCATTGGATTCTTGCTAAATTGGACATCTGATTAACAATATGACTACATGTTCAAGTTCTATAGCTGCAAATTTGTTGAACTATGGTTTATTTTGAATCTCAAATTCCTGAAGGCTGTGCATTTGCTAAATTTGCAGTCGGAGTTGAGGAAGACAGAGCTCAACAAAGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCAATTGTATGTACTTTTCTAGGACGCCTCTTGAGGTAGTAAAGATTTCTCGTGTTATTATATGAGTGTCATCTTCTGCATAAGGAGGGATTGGTATATAGCTTTGAAGTTAGAATAATTGTATAGGTTCTATTCCCCTTATGGCTCCCTCCCGGTTTTCGGATACCGCTTCTGTTGTGAAGTGAATTTGAACATAACTTAATTAGTTCGACATTAATGTCACTCCCAACTTGAGTATGGCATAATGTGGGAAGTTATCGATGCTCACGAGTCTGAATTGATATACATTTTAGATGTGTTATAGTGCTAATATTAATATTGTTATATTATTGTAGACATCTTTGTCAGTCT

mRNA sequence

TAACAAGAAAGTCGATTGACATGCAGAACAAAGGAACTCTTAATAACATGTAACTCCGTCACTTCGCTCCTTCTTCTCGGTCCACAGCACAAACAGCTTTCCTTCTCTTTCCGAATCACTTCTATTCCTTTCTCCACTTTTCTCTGTGTTCCTTTCTGATTCCCTATTCCAATCCTTTACCCCTTTGGATTTATATCATGAAACTCCCTTCCTTGCTTTTTCTTTTTCAATCACAATATTCCATCACTTTCTGCTATTCACTTCACTCTGTTTTGTTCGTATACCGTTCGAAATGATGACCCAATTCATCAACTAATCCCCCACTTCTTTTCTTTCAACTTTTTGAAGCTGATCAGTTGAGTTCTGCTGACCGGCCAACTCATTGTGTTGTCGCCAATTTCTTGTTGATTGTAATTTGACAAGTGTTTGGTTTAATAGCTGAGTGAAAGTATGGATATGAGGCTTTTAGAAATTCATCCTCGCGAATTGAAGTTCATTTTTGAACTAAATAAACATAGCTTATGCTCAATTCAACTCACAAATAATACACTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCGAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGTGCCAAACTCAACTTGCGAATTCACGGTTAGGATGCAAGCACAAACGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGTGGACATAACCTCTACCTTGTTTGCCAAAGAAGGTGGGAAGTACATCGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCATTTCTCCAGTGGTTTCCCCAGTTGATGAAGCATTAAAGGAGGAGGAAGTGGCTCTTGCAGTTACAGAACCAAATAATCTATGTAAAGCAGTGGAGGAGATTGTAGACCAGGAACCTTTAAACAAAAACCATGTTCCCGGCAATGCTACGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCATCATGTTTACCTAATGACTTCATAGATGGCAATGAGGAGAATCTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTGAGTGAACTCGAGTTGAAGCTTTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGAAGGAGAATTTTTCGGAGTTGAGGAAGACAGAGCTCAACAAAGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCAATTGTATGTACTTTTCTAGGACGCCTCTTGAGGTAGTAAAGATTTCTCGTGTTATTATATGAGTGTCATCTTCTGCATAAGGAGGGATTGGTATATAGCTTTGAAGTTAGAATAATTGTATAGGTTCTATTCCCCTTATGGCTCCCTCCCGGTTTTCGGATACCGCTTCTGTTGTGAAGTGAATTTGAACATAACTTAATTAGTTCGACATTAATGTCACTCCCAACTTGAGTATGGCATAATGTGGGAAGTTATCGATGCTCACGAGTCTGAATTGATATACATTTTAGATGTGTTATAGTGCTAATATTAATATTGTTATATTATTGTAGACATCTTTGTCAGTCT

Coding sequence (CDS)

ATGGATATGAGGCTTTTAGAAATTCATCCTCGCGAATTGAAGTTCATTTTTGAACTAAATAAACATAGCTTATGCTCAATTCAACTCACAAATAATACACTCGACCATGTTGCTTTTAAGGTTAAGATCACATCCCCGAAGAAGTACTTTGTCCGACCAAAGGTTGGAATCATCGTGCCAAACTCAACTTGCGAATTCACGGTTAGGATGCAAGCACAAACGCTGGCCCCACCTGATATGCTATGCAAAGACAAGTTCTTAATCCAGAGCACAGTAGTTCCTGCTGATACAACTGATGTGGACATAACCTCTACCTTGTTTGCCAAAGAAGGTGGGAAGTACATCGAAGAACAGAAGTTGGGTGTAACCCTTGTTAGCCCATCCATTTCTCCAGTGGTTTCCCCAGTTGATGAAGCATTAAAGGAGGAGGAAGTGGCTCTTGCAGTTACAGAACCAAATAATCTATGTAAAGCAGTGGAGGAGATTGTAGACCAGGAACCTTTAAACAAAAACCATGTTCCCGGCAATGCTACGGAGCCTAGAGATGTCAATGAAGAGGAGCCTGAACCATCATGTTTACCTAATGACTTCATAGATGGCAATGAGGAGAATCTGGAAATGATAAAGCATCTCGAGGAGTTGAAGTTAAAGCTGAGTGAACTCGAGTTGAAGCTTTGTCAGGCTCAAAATGCCATTTCAAAGCTAAAGGAGGAGAAGCAAATAAGCATCCAAGAAACCAAATTCTTGAAGGAGAATTTTTCGGAGTTGAGGAAGACAGAGCTCAACAAAGTTCAAGTTGGCTTTCCTGTTCTGTATCTCTACATGGTGGCAATTGTATGTACTTTTCTAGGACGCCTCTTGAGGTAG

Protein sequence

MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR
Homology
BLAST of Bhi02G001764 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-47
Identity = 148/385 (38.44%), Postives = 196/385 (50.91%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+M LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
            STCEFTV MQA    PPDM+CKDKFLIQST V A+TTD DIT+++F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSISPVVSPV----------DEALKE----EEVALA--------VTEPN----- 180
            VTLV PS SP +SP+          D  LK+    +   LA        V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVDQ---------EPL--NKNHVP 240
                                       N  KA ++  D          +P+  +K+   
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 GNATE---------------PRDVN-------EEEP----EPSCLPNDFIDGNE----EN 289
           G A +               PRD         + EP    E   +     DG      + 
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of Bhi02G001764 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-47
Identity = 148/385 (38.44%), Postives = 196/385 (50.91%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+M LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
            STCEFTV MQA    PPDM+CKDKFLIQST V A+TTD DIT+++F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSISPVVSPV----------DEALKE----EEVALA--------VTEPN----- 180
            VTLV PS SP +SP+          D  LK+    +   LA        V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVDQ---------EPL--NKNHVP 240
                                       N  KA ++  D          +P+  +K+   
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 GNATE---------------PRDVN-------EEEP----EPSCLPNDFIDGNE----EN 289
           G A +               PRD         + EP    E   +     DG      + 
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of Bhi02G001764 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 138.3 bits (347), Expect = 9.9e-33
Identity = 98/284 (34.51%), Postives = 138/284 (48.59%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNSTC 64
           LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G+++P STC
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVTL 124
           E  V MQAQ  AP DM CKDKFL+Q  +     T  ++T  +F+KE G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEPRDVN 184
           V+P   P  SPV E                                             +
Sbjct: 142 VAPPRPP--SPVHEG--------------------------------------------S 201

Query: 185 EEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQ 244
           EE   P    +D   G+E + E  + + + K    E      +A+  I+KL EEKQ +IQ
Sbjct: 202 EEGSSPRASVSDNGHGSEFSFE--RFIVDNKAGHQE---NTSEARALITKLTEEKQSAIQ 254

Query: 245 ETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
               L+    +LR+        G P +Y+ +V ++   LG +++
Sbjct: 262 LNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of Bhi02G001764 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.8e-32
Identity = 71/138 (51.45%), Postives = 90/138 (65.22%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNSTC 64
           LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G+++P STC
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVTL 124
           E  V MQAQ  AP DM CKDKFL+Q  +     T  ++T  +F+KE G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSISPVVSPVDEALKE 143
           V+P   P  SPV E  +E
Sbjct: 142 VAPPRPP--SPVHEGSEE 157

BLAST of Bhi02G001764 vs. TAIR 10
Match: AT3G60600.3 (vesicle associated protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.8e-32
Identity = 71/138 (51.45%), Postives = 90/138 (65.22%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNSTC 64
           LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G+++P STC
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVTL 124
           E  V MQAQ  AP DM CKDKFL+Q  +     T  ++T  +F+KE G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSISPVVSPVDEALKE 143
           V+P   P  SPV E  +E
Sbjct: 142 VAPPRPP--SPVHEGSEE 157

BLAST of Bhi02G001764 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.5e-46
Identity = 148/385 (38.44%), Postives = 196/385 (50.91%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+M LL+I PR L+F  +L K + C +QLTN T  +VAFKVK TSPKKY VRP VG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
            STCEFTV MQA    PPDM+CKDKFLIQST V A+TTD DIT+++F+K  GK+IEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 GVTLVSPSISPVVSPV----------DEALKE----EEVALA--------VTEPN----- 180
            VTLV PS SP +SP+          D  LK+    +   LA        V EP      
Sbjct: 121 RVTLVPPSDSPELSPINTPKQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 180

Query: 181 ----------------------------NLCKAVEEIVDQ---------EPL--NKNHVP 240
                                       N  KA ++  D          +P+  +K+   
Sbjct: 181 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 240

Query: 241 GNATE---------------PRDVN-------EEEP----EPSCLPNDFIDGNE----EN 289
           G A +               PRD         + EP    E   +     DG      + 
Sbjct: 241 GRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGLDE 300

BLAST of Bhi02G001764 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-31
Identity = 98/284 (34.51%), Postives = 138/284 (48.59%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNSTC 64
           LL + P +L+F FEL K   CS+ LTN T ++VAFKVK T+PKKY VRP  G+++P STC
Sbjct: 22  LLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTC 81

Query: 65  EFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVTL 124
           E  V MQAQ  AP DM CKDKFL+Q  +     T  ++T  +F+KE G  +EE KL VT 
Sbjct: 82  EVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTY 141

Query: 125 VSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEPRDVN 184
           V+P   P  SPV E                                             +
Sbjct: 142 VAPPRPP--SPVHEG--------------------------------------------S 201

Query: 185 EEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISIQ 244
           EE   P    +D   G+E + E  + + + K    E      +A+  I+KL EEKQ +IQ
Sbjct: 202 EEGSSPRASVSDNGHGSEFSFE--RFIVDNKAGHQE---NTSEARALITKLTEEKQSAIQ 254

Query: 245 ETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
               L+    +LR+        G P +Y+ +V ++   LG +++
Sbjct: 262 LNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMK 254

BLAST of Bhi02G001764 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.5e-30
Identity = 65/125 (52.00%), Postives = 85/125 (68.00%), Query Frame = 0

Query: 4   RLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVPNST 63
           +L+ I P ELKF+FEL K S C +++ N T ++VAFKVK TSPKKYFVRP  G+I P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  CEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVT 123
           C   V +QAQ   PPDM CKDKFL+QST+VP  T   ++    F K+ GK + E KL V+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 LVSPS 129
            ++PS
Sbjct: 128 YITPS 132

BLAST of Bhi02G001764 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 101/284 (35.56%), Postives = 139/284 (48.94%), Query Frame = 0

Query: 5   LLEIHPRELKFIFELNKHSLCSIQLTNNTLDH-VAFKVKITSPKKYFVRPKVGIIVPNST 64
           L+ IHP ELKF FEL K S CS+QLTN T    VAFKVK T+P+KY VRP  G+++P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  CEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKLGVT 124
           C  TV MQAQ  AP DM CKDKFL+Q+ VV   TT  ++ + +F KE G+ IE+ KL V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEPRDV 184
            + P+  P  SPV E  +E    +A                                  +
Sbjct: 126 YI-PANPP--SPVPEGSEEGNSPMA---------------------------------SL 185

Query: 185 NEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQISI 244
           N+   + + L +D     EE  E                 K  +A + ISKL EEK  + 
Sbjct: 186 NDIASQSASLFDDVSRTFEETSE-----------------KSSEAWSMISKLTEEKTSAT 236

Query: 245 QETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLL 288
           Q+++ L+     LRK    K   G  +L + +V ++   +G LL
Sbjct: 246 QQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 236

BLAST of Bhi02G001764 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.0e-29
Identity = 96/292 (32.88%), Postives = 135/292 (46.23%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M   LL I P +L+F FEL K   CS+ L N T ++VAFKVK T+PKKY VRP  G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
            S+ E  V MQAQ  AP D+ CKDKFL+Q  V     T  D+T  +F+KE G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATE- 180
            V  V+P   P  SPV E  +E     A    N                     GNA++ 
Sbjct: 121 RVVYVAPPRPP--SPVREGSEEGSSPRASVSDN---------------------GNASDF 180

Query: 181 ---PRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLK 240
              PR              D +D  + + E                     A+  ++KL 
Sbjct: 181 TAAPR-----------FSADRVDAQDNSSE---------------------ARALVTKLT 237

Query: 241 EEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           EEK  ++Q    L++   +LR+        G P +Y+ +V ++   LG +++
Sbjct: 241 EEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMK 237

BLAST of Bhi02G001764 vs. NCBI nr
Match: XP_038878362.1 (vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 2.8e-154
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP
Sbjct: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL
Sbjct: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180
           GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP
Sbjct: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180

Query: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
           RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR
Sbjct: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 288

BLAST of Bhi02G001764 vs. NCBI nr
Match: XP_011659931.1 (vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740596.1 vesicle-associated protein 2-1 [Cucumis sativus] >XP_031740621.1 vesicle-associated protein 2-1 [Cucumis sativus] >KGN66132.1 hypothetical protein Csa_007310 [Cucumis sativus])

HSP 1 Score: 454.5 bits (1168), Expect = 6.7e-124
Identity = 243/288 (84.38%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+ RLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKYFVRPKVGII P
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQ Q LAPPDMLCKDKFLIQSTVVPADTTD DI S+LFAKEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180
           GV LV+PS SPV SP DEA            PNNLC+AVEEIVDQEPLNKNHVP N TEP
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
           RDV E+EPEP+   NDFID NE+N+EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           ISIQETKFL+ENFSELRKTELN+V+VGFPVLYLYMVA+VCTFLGRLLR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of Bhi02G001764 vs. NCBI nr
Match: XP_008450729.1 (PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo])

HSP 1 Score: 452.6 bits (1163), Expect = 2.5e-123
Identity = 241/288 (83.68%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+ RLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKYFVRPKVGII P
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST  FTVRMQ Q LAPPDMLCKDKFLIQSTVVPADTTD DI S+LF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180
           GV LV+PSISPV +PVDEA             NNLCKAVEEIVDQEPLNKNHVP N TEP
Sbjct: 121 GVILVTPSISPVFTPVDEA------------SNNLCKAVEEIVDQEPLNKNHVPDNKTEP 180

Query: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
           RDV E+EPEP+ L N+FID NE+N EMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASLANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQ 240

Query: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           ISIQETKFL+ENFSELRK ELN+VQVGFPVLYLYMVA+VCTFLGRLLR
Sbjct: 241 ISIQETKFLQENFSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of Bhi02G001764 vs. NCBI nr
Match: KAG7023965.1 (Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432.2 bits (1110), Expect = 3.6e-117
Identity = 239/302 (79.14%), Postives = 255/302 (84.44%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQAQ LA PDMLCKD+FLIQSTVVPADTTD DITS+LFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNH-------- 180
           GVTLVSPSIS       +ALKEEE AL VT PNNL  AVEEIVDQEP +++H        
Sbjct: 121 GVTLVSPSIS-------QALKEEE-ALGVTGPNNLSTAVEEIVDQEPSHESHVVSNGVDS 180

Query: 181 ------VPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLC 240
                 VPGN T+PRDV EEEPEP+ L ND +DG+ ENLEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRL 289
           +AQNAISKLKEEKQISIQE KFL+ENFSELRKTEL +VQVGFPVLYLYMVA+VCTFLGR 
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of Bhi02G001764 vs. NCBI nr
Match: XP_023545303.1 (vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545304.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545305.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545306.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545307.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545308.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.0 bits (1107), Expect = 7.9e-117
Identity = 238/302 (78.81%), Postives = 255/302 (84.44%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQAQ LA PDMLCKD+FLIQSTVVPADTTD DITS+LFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNH-------- 180
           GVTLVSPSIS       +ALKEEE AL VT PNNL  AVEEIVDQEP +++H        
Sbjct: 121 GVTLVSPSIS-------QALKEEE-ALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 ------VPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLC 240
                 VPGN T+PRDV EEEPEP+ L ND +DG+ ENLEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTDPRDVGEEEPEPASLANDVVDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRL 289
           +AQN+ISKLKEEKQISIQE KFL+ENFSELRKTEL +VQVGFPVLYLYMVA+VCTFLGR 
Sbjct: 241 EAQNSISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of Bhi02G001764 vs. ExPASy TrEMBL
Match: A0A0A0LZ53 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.2e-124
Identity = 243/288 (84.38%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+ RLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKYFVRPKVGII P
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQ Q LAPPDMLCKDKFLIQSTVVPADTTD DI S+LFAKEGGKYIEEQKL
Sbjct: 61  NSTREFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIVSSLFAKEGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180
           GV LV+PS SPV SP DEA            PNNLC+AVEEIVDQEPLNKNHVP N TEP
Sbjct: 121 GVILVTPS-SPVFSPADEA------------PNNLCEAVEEIVDQEPLNKNHVPDNNTEP 180

Query: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
           RDV E+EPEP+   NDFID NE+N+EMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASSANDFIDSNEDNVEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240

Query: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           ISIQETKFL+ENFSELRKTELN+V+VGFPVLYLYMVA+VCTFLGRLLR
Sbjct: 241 ISIQETKFLQENFSELRKTELNRVKVGFPVLYLYMVALVCTFLGRLLR 275

BLAST of Bhi02G001764 vs. ExPASy TrEMBL
Match: A0A1S3BPW9 (vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 241/288 (83.68%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           M+ RLLEIHPRELKFIFELNK SLCSIQLTN T+DHVAFKVKITSPKKYFVRPKVGII P
Sbjct: 1   METRLLEIHPRELKFIFELNKDSLCSIQLTNKTVDHVAFKVKITSPKKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST  FTVRMQ Q LAPPDMLCKDKFLIQSTVVPADTTD DI S+LF KEGGKYIEEQKL
Sbjct: 61  NSTRGFTVRMQGQQLAPPDMLCKDKFLIQSTVVPADTTDGDIISSLFVKEGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNHVPGNATEP 180
           GV LV+PSISPV +PVDEA             NNLCKAVEEIVDQEPLNKNHVP N TEP
Sbjct: 121 GVILVTPSISPVFTPVDEA------------SNNLCKAVEEIVDQEPLNKNHVPDNKTEP 180

Query: 181 RDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLCQAQNAISKLKEEKQ 240
           RDV E+EPEP+ L N+FID NE+N EMIKHLEELKLKLSELELKLCQAQN+ISKLKEEKQ
Sbjct: 181 RDVVEKEPEPASLANNFIDSNEDNAEMIKHLEELKLKLSELELKLCQAQNSISKLKEEKQ 240

Query: 241 ISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRLLR 289
           ISIQETKFL+ENFSELRK ELN+VQVGFPVLYLYMVA+VCTFLGRLLR
Sbjct: 241 ISIQETKFLQENFSELRKKELNRVQVGFPVLYLYMVALVCTFLGRLLR 276

BLAST of Bhi02G001764 vs. ExPASy TrEMBL
Match: A0A6J1I0M1 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467905 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.8e-117
Identity = 239/302 (79.14%), Postives = 256/302 (84.77%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHP+ELKFI E NKHSLCSIQLTNN L+HVAFKVK TSP KYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLEHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQAQ LA PDMLCKD+FLIQSTVVPADTTD DITS+LFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNH-------- 180
           GVTLVSPSIS       +ALKEEE AL VT PNNL  AVEEIVDQEP +++H        
Sbjct: 121 GVTLVSPSIS-------QALKEEE-ALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 ------VPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLC 240
                 VPGN T+PRDV EEEPEP+ L ND IDG+ ENLE+IK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTDPRDVGEEEPEPASLANDVIDGDLENLEIIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRL 289
           +AQNAISKLKEEKQISIQE KFL+ENFSELRKTEL +VQVGFPVLYLYMVA+VCTFLGRL
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRL 294

BLAST of Bhi02G001764 vs. ExPASy TrEMBL
Match: A0A6J1HAA2 (vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.0e-117
Identity = 239/302 (79.14%), Postives = 254/302 (84.11%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQAQ LA PDMLCKD+FLIQSTVVPADTTD DITS+LFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNH-------- 180
           GVTL+SPSIS       +ALKEEE AL VT PNNL  AVEEIVDQEP +++H        
Sbjct: 121 GVTLISPSIS-------QALKEEE-ALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 ------VPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLC 240
                 VPGN T PRDV EEEPEP+ L ND IDG+ ENLEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRL 289
           +AQNAISKLKEEKQISIQE KFL+ENFSELRKTEL +VQVGFPVLYLYMVA+VCTFLGR 
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

BLAST of Bhi02G001764 vs. ExPASy TrEMBL
Match: A0A6J1HBX8 (vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462041 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 5.0e-117
Identity = 239/302 (79.14%), Postives = 254/302 (84.11%), Query Frame = 0

Query: 1   MDMRLLEIHPRELKFIFELNKHSLCSIQLTNNTLDHVAFKVKITSPKKYFVRPKVGIIVP 60
           MDMRLLEIHP+ELKFI E NKHSLCSIQLTNN LDHVAFKVK TSP KYFVRPKVGII P
Sbjct: 1   MDMRLLEIHPQELKFICEPNKHSLCSIQLTNNMLDHVAFKVKTTSPDKYFVRPKVGIIEP 60

Query: 61  NSTCEFTVRMQAQTLAPPDMLCKDKFLIQSTVVPADTTDVDITSTLFAKEGGKYIEEQKL 120
           NST EFTVRMQAQ LA PDMLCKD+FLIQSTVVPADTTD DITS+LFAK+GGKYIEEQKL
Sbjct: 61  NSTREFTVRMQAQKLALPDMLCKDRFLIQSTVVPADTTDEDITSSLFAKDGGKYIEEQKL 120

Query: 121 GVTLVSPSISPVVSPVDEALKEEEVALAVTEPNNLCKAVEEIVDQEPLNKNH-------- 180
           GVTL+SPSIS       +ALKEEE AL VT PNNL  AVEEIVDQEP +++H        
Sbjct: 121 GVTLISPSIS-------QALKEEE-ALGVTGPNNLSTAVEEIVDQEPSHESHVMSNGVDS 180

Query: 181 ------VPGNATEPRDVNEEEPEPSCLPNDFIDGNEENLEMIKHLEELKLKLSELELKLC 240
                 VPGN T PRDV EEEPEP+ L ND IDG+ ENLEMIK LEELKLKLSELELKLC
Sbjct: 181 LPPKHMVPGNGTVPRDVGEEEPEPASLANDVIDGDLENLEMIKDLEELKLKLSELELKLC 240

Query: 241 QAQNAISKLKEEKQISIQETKFLKENFSELRKTELNKVQVGFPVLYLYMVAIVCTFLGRL 289
           +AQNAISKLKEEKQISIQE KFL+ENFSELRKTEL +VQVGFPVLYLYMVA+VCTFLGR 
Sbjct: 241 EAQNAISKLKEEKQISIQENKFLQENFSELRKTELKRVQVGFPVLYLYMVALVCTFLGRF 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G08820.11.1e-4738.44vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.21.1e-4738.44vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.19.9e-3334.51vesicle associated protein [more]
AT3G60600.23.8e-3251.45vesicle associated protein [more]
AT3G60600.33.8e-3251.45vesicle associated protein [more]
Match NameE-valueIdentityDescription
B9DHD71.5e-4638.44Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ951.4e-3134.51Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9LVU14.5e-3052.00Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Q84WW51.3e-2935.56Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9SHC85.0e-2932.88Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_038878362.12.8e-154100.00vesicle-associated protein 1-3-like isoform X1 [Benincasa hispida][more]
XP_011659931.16.7e-12484.38vesicle-associated protein 2-1 [Cucumis sativus] >XP_011659932.1 vesicle-associa... [more]
XP_008450729.12.5e-12383.68PREDICTED: vesicle-associated protein 1-3-like [Cucumis melo][more]
KAG7023965.13.6e-11779.14Vesicle-associated protein 2-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023545303.17.9e-11778.81vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ533.2e-12484.38MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G573020 PE=4 SV... [more]
A0A1S3BPW91.2e-12383.68vesicle-associated protein 1-3-like OS=Cucumis melo OX=3656 GN=LOC103492221 PE=4... [more]
A0A6J1I0M13.8e-11779.14vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HAA25.0e-11779.14vesicle-associated protein 2-1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HBX85.0e-11779.14vesicle-associated protein 2-1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 204..252
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..190
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..287
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..103
e-value: 1.7E-26
score: 92.2
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 26.82091
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 2..128
e-value: 1.4E-38
score: 133.7
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..288
e-value: 3.1E-46
score: 156.6
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..287
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 5..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001764Bhi02M001764mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane