Bhi02G001706 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001706
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectinesterase
Locationchr2: 56183144 .. 56185541 (+)
RNA-Seq ExpressionBhi02G001706
SyntenyBhi02G001706
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTAGAGGGGGCTCCTAAGAGGAAGGTCCCCAACTAGCTAAAAAAAAAAAAACCCTCCCTTTTTTTTTCATTCTTTTAATTATTTTTCAACCTCTCCTTGATTTCTAAAGGGAGATACTTTATTTTTAAAAAAAAAGAAGGAAAAAAAAAACCTTTTTTGGTCATTCGAAGAGAAAAAAGAAAAAAAAAAAAAGAGTTCTTTTTTTTGTTTAGGACAAAATGAGTTATGGGGATGATGATGCTCAGAACAAGAAAAGATATGCCATCATCGGCGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACAGTTGGTGTTAACCTAAACCAAGGGGAAACGAGCGATCCGGCCATTGGGAGCAAGTCTCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGACTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGTAACAACTCTTCAGATCCAAAGGAATTGGTTCAAGCTGGCTTCAAAGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGATTGAGAAAGACCCTAGAACCTCCAAAGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCCGTTGATGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATTGATATTAAGATCTGGCTCAGCGCTACCATTACTTATCAGGTAATGTAGTTCAGGTTAAATGTTTAAGCTCCTTAAAGTAAATTTATGATTTATGTTCAATGTTTGTATTGGTTTAGGAAACTTGCTTGGATGGGTTTGAGAACACAACAGGAAATGCAGCAGAGAAAATGAAGAAGGCATTGAAGACGTCGATGAAGTTGAGCAGCAATGGGTTGGCGATGGTGTCGCAGATCTCGTCCATGTTGACAGAGTTGCAAATTCCAGGGATTAGCCGAAGGCGACTTCTTGAGATTCCGGTTCTTGGACACGATGATTACCCCGATTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACAAGAGTGAAGCCCAATGTTGTGGTGGCTAAGGATGGAAGTGGTCAGTTCAGGACTATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACTTATGTGATTCATATCAAGCAAGGAGTGTATAAGGAATATCTCATGATTAAAAAATCCCTCACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATTATCACTGGAAGCAAGAACTTTATTGATGGCACACCCACCTTCAAAACTGCAACTGTTGGTGAGTATCCTTCAAATTTAGTTCTTATAATTCGATATTCAACGAGAGATTAACTGTTTGATTTGTGTGTTGTATATAGCTGTGACAGCAGAGCACTTCATGGCAAGAGACATCGGGTTCGAGAACACGGCAGGGCCACATAAGCATCAAGCCGTCGCATTAAGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGTGAAATGCATGGTTACCAAGACACTCTCTACGTCCACACCATGCGTCAATTCTACCGCGATTGCACCATCTCCGGTACCATCGACTTCGTCTTCGGCGACGCAGCGGCCGTCTTCCAAAGCTGCACCTTCCTAGTCCGCAAGCCACTTCCAAACCAGCAATGCATCGTCACGGCACATGGACGCAAGGAGCGCCGCCAACCATCCGCCCTCATTATCCAAAACAGTTCCTTCAAGGCCGACGCCGACCTCGCCCCAGTGCAAAAGCAGTTCCGATCCTTCCTTGGACGACCATGGAAGGAATATTCTAGAACAATCATCATGGAATCATACATTGGTGACTTGATTCAGCCTGAAGGTTGGTTGCCATGGGCTGGAGATTGGGGCTTGAAGACATGTTTTTACACAGAGTATAATAATTATGGGCCAGGATCTGATAAATCAAAGAGGGTGAAATGGAGAGGGATTAAGACAATTACACCACAACATGCTGTAGATTTTACTGCCGGAAGGTTTCTTAGAGGGGATAGTTGGATTAAGTCTACCGGAGTTCCTTATGTTTCCGGCATGACCAGAACCCGCGCCGCCGCTGCAAGGGGCGGAGCTACTCATTGATTTTTTGTTGTTTCAACATTTATTTGTTATGTAATGGCGGCCGGCCGTCTCTCTGGCTTGGCTCAATCCTTTTTTTCCCTTTTTCTGTCTCTCTAATGTGGTTTAAGACTTTTAAGTTGTGTGTTCATGTGTGGGTCTAATTCTATATAGTGGAAGAAAATATTTTCTTCTTTTTTCTTTTTATGGTTACATTTGTTTGCAATTCTTCCATTTTTTTTCTTTTTTTTTTTAGAGTTTGGGTTTGGACCTTTTTGTTTCTTT

mRNA sequence

CTCTAGAGGGGGCTCCTAAGAGGAAGGTCCCCAACTAGCTAAAAAAAAAAAAACCCTCCCTTTTTTTTTCATTCTTTTAATTATTTTTCAACCTCTCCTTGATTTCTAAAGGGAGATACTTTATTTTTAAAAAAAAAGAAGGAAAAAAAAAACCTTTTTTGGTCATTCGAAGAGAAAAAAGAAAAAAAAAAAAAGAGTTCTTTTTTTTGTTTAGGACAAAATGAGTTATGGGGATGATGATGCTCAGAACAAGAAAAGATATGCCATCATCGGCGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACAGTTGGTGTTAACCTAAACCAAGGGGAAACGAGCGATCCGGCCATTGGGAGCAAGTCTCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGACTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGTAACAACTCTTCAGATCCAAAGGAATTGGTTCAAGCTGGCTTCAAAGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGATTGAGAAAGACCCTAGAACCTCCAAAGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCCGTTGATGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATTGATATTAAGATCTGGCTCAGCGCTACCATTACTTATCAGGAAACTTGCTTGGATGGGTTTGAGAACACAACAGGAAATGCAGCAGAGAAAATGAAGAAGGCATTGAAGACGTCGATGAAGTTGAGCAGCAATGGGTTGGCGATGGTGTCGCAGATCTCGTCCATGTTGACAGAGTTGCAAATTCCAGGGATTAGCCGAAGGCGACTTCTTGAGATTCCGGTTCTTGGACACGATGATTACCCCGATTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACAAGAGTGAAGCCCAATGTTGTGGTGGCTAAGGATGGAAGTGGTCAGTTCAGGACTATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACTTATGTGATTCATATCAAGCAAGGAGTGTATAAGGAATATCTCATGATTAAAAAATCCCTCACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATTATCACTGGAAGCAAGAACTTTATTGATGGCACACCCACCTTCAAAACTGCAACTGTTGCTGTGACAGCAGAGCACTTCATGGCAAGAGACATCGGGTTCGAGAACACGGCAGGGCCACATAAGCATCAAGCCGTCGCATTAAGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGTGAAATGCATGGTTACCAAGACACTCTCTACGTCCACACCATGCGTCAATTCTACCGCGATTGCACCATCTCCGGTACCATCGACTTCGTCTTCGGCGACGCAGCGGCCGTCTTCCAAAGCTGCACCTTCCTAGTCCGCAAGCCACTTCCAAACCAGCAATGCATCGTCACGGCACATGGACGCAAGGAGCGCCGCCAACCATCCGCCCTCATTATCCAAAACAGTTCCTTCAAGGCCGACGCCGACCTCGCCCCAGTGCAAAAGCAGTTCCGATCCTTCCTTGGACGACCATGGAAGGAATATTCTAGAACAATCATCATGGAATCATACATTGGTGACTTGATTCAGCCTGAAGGTTGGTTGCCATGGGCTGGAGATTGGGGCTTGAAGACATGTTTTTACACAGAGTATAATAATTATGGGCCAGGATCTGATAAATCAAAGAGGGTGAAATGGAGAGGGATTAAGACAATTACACCACAACATGCTGTAGATTTTACTGCCGGAAGGTTTCTTAGAGGGGATAGTTGGATTAAGTCTACCGGAGTTCCTTATGTTTCCGGCATGACCAGAACCCGCGCCGCCGCTGCAAGGGGCGGAGCTACTCATTGATTTTTTGTTGTTTCAACATTTATTTGTTATGTAATGGCGGCCGGCCGTCTCTCTGGCTTGGCTCAATCCTTTTTTTCCCTTTTTCTGTCTCTCTAATGTGGTTTAAGACTTTTAAGTTGTGTGTTCATGTGTGGGTCTAATTCTATATAGTGGAAGAAAATATTTTCTTCTTTTTTCTTTTTATGGTTACATTTGTTTGCAATTCTTCCATTTTTTTTCTTTTTTTTTTTAGAGTTTGGGTTTGGACCTTTTTGTTTCTTT

Coding sequence (CDS)

ATGAGTTATGGGGATGATGATGCTCAGAACAAGAAAAGATATGCCATCATCGGCGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACAGTTGGTGTTAACCTAAACCAAGGGGAAACGAGCGATCCGGCCATTGGGAGCAAGTCTCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGACTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGTAACAACTCTTCAGATCCAAAGGAATTGGTTCAAGCTGGCTTCAAAGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGATTGAGAAAGACCCTAGAACCTCCAAAGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCCGTTGATGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATTGATATTAAGATCTGGCTCAGCGCTACCATTACTTATCAGGAAACTTGCTTGGATGGGTTTGAGAACACAACAGGAAATGCAGCAGAGAAAATGAAGAAGGCATTGAAGACGTCGATGAAGTTGAGCAGCAATGGGTTGGCGATGGTGTCGCAGATCTCGTCCATGTTGACAGAGTTGCAAATTCCAGGGATTAGCCGAAGGCGACTTCTTGAGATTCCGGTTCTTGGACACGATGATTACCCCGATTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACAAGAGTGAAGCCCAATGTTGTGGTGGCTAAGGATGGAAGTGGTCAGTTCAGGACTATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACTTATGTGATTCATATCAAGCAAGGAGTGTATAAGGAATATCTCATGATTAAAAAATCCCTCACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATTATCACTGGAAGCAAGAACTTTATTGATGGCACACCCACCTTCAAAACTGCAACTGTTGCTGTGACAGCAGAGCACTTCATGGCAAGAGACATCGGGTTCGAGAACACGGCAGGGCCACATAAGCATCAAGCCGTCGCATTAAGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGTGAAATGCATGGTTACCAAGACACTCTCTACGTCCACACCATGCGTCAATTCTACCGCGATTGCACCATCTCCGGTACCATCGACTTCGTCTTCGGCGACGCAGCGGCCGTCTTCCAAAGCTGCACCTTCCTAGTCCGCAAGCCACTTCCAAACCAGCAATGCATCGTCACGGCACATGGACGCAAGGAGCGCCGCCAACCATCCGCCCTCATTATCCAAAACAGTTCCTTCAAGGCCGACGCCGACCTCGCCCCAGTGCAAAAGCAGTTCCGATCCTTCCTTGGACGACCATGGAAGGAATATTCTAGAACAATCATCATGGAATCATACATTGGTGACTTGATTCAGCCTGAAGGTTGGTTGCCATGGGCTGGAGATTGGGGCTTGAAGACATGTTTTTACACAGAGTATAATAATTATGGGCCAGGATCTGATAAATCAAAGAGGGTGAAATGGAGAGGGATTAAGACAATTACACCACAACATGCTGTAGATTTTACTGCCGGAAGGTTTCTTAGAGGGGATAGTTGGATTAAGTCTACCGGAGTTCCTTATGTTTCCGGCATGACCAGAACCCGCGCCGCCGCTGCAAGGGGCGGAGCTACTCATTGA

Protein sequence

MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYPDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH
Homology
BLAST of Bhi02G001706 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 653.7 bits (1685), Expect = 1.4e-187
Identity = 312/575 (54.26%), Postives = 432/575 (75.13%), Query Frame = 0

Query: 1   MSYG--DDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASV 60
           MSYG  D+DA+ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI+ SV
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSV 60

Query: 61  KAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEK 120
           KAIK +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS T+ E++K
Sbjct: 61  KAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQK 120

Query: 121 DPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLD 180
           DPR   AL  CKELMD+A+ EL  S  +LG+F+  K+DE L+ ++IWLSATI++++TCLD
Sbjct: 121 DPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLD 180

Query: 181 GFENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHD 240
           GF+ T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        
Sbjct: 181 GFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------Q 240

Query: 241 DYPDWANPGMRRLL-AAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQ 300
           ++P W +   RRLL A  + VKP++VVA+DGSGQ++TI EAL+ VPK+K N T+V+HIK+
Sbjct: 241 EFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKE 300

Query: 301 GVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGF 360
           G+YKEY+ + +S+THL+ IGDGP KT+I+GSK++ DG  T+KTATVA+  +HF+A++I F
Sbjct: 301 GIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAF 360

Query: 361 ENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDA 420
           ENTAG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDA
Sbjct: 361 ENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDA 420

Query: 421 AAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFL 480
           AAVFQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q  +   + D   V++Q +++L
Sbjct: 421 AAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYL 480

Query: 481 GRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRG 540
           GRPWKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W G
Sbjct: 481 GRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPG 540

Query: 541 IKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGM 573
           IK ++ +  + FT  ++++GD+WI   GVPY+ G+
Sbjct: 541 IKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Bhi02G001706 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 652.1 bits (1681), Expect = 4.2e-187
Identity = 316/586 (53.92%), Postives = 437/586 (74.57%), Query Frame = 0

Query: 1   MSYG-DDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ--GETSDPAIGSKSHEISAS 60
           MSYG DD+++ K+RY +I +SS+LL++MVVAVTVGV+LN+  G++   A      E++AS
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA------EVNAS 60

Query: 61  VKAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIE 120
           VKA+K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS T+ E++
Sbjct: 61  VKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQ 120

Query: 121 KDPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCL 180
           KD RT  AL  CKELMD+A+DEL  S  +LG+F+   LDE LI+++IWLSA I+++ETCL
Sbjct: 121 KDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCL 180

Query: 181 DGFENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGH 240
           +GF+ T GNA E MKKALKT+++L+ NGLA++S++S+ + ++QIPG++ RRLL       
Sbjct: 181 EGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------ 240

Query: 241 DDYPDWANPGMRRLL---AAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIH 300
           + +P W +   R+LL   AA + VKP++VVA+DGSGQ++TI EAL  VPK K N T+V+H
Sbjct: 241 EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVH 300

Query: 301 IKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARD 360
           IK G+YKEY+ + K+++HL+ IGDGP KTII+G+KN+ DG  T++TATVA+   +F+A++
Sbjct: 301 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKN 360

Query: 361 IGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVF 420
           IGFENTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCTISGTIDF+F
Sbjct: 361 IGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLF 420

Query: 421 GDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFR 480
           GDAAAVFQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q  +   + D   V++  +
Sbjct: 421 GDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSK 480

Query: 481 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVK 540
           ++LGRPWKEYSRTIIM ++I D +QP+GW PW GD+GLKT FY+E  N GPGS  + RV 
Sbjct: 481 AYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVT 540

Query: 541 WRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           W GIKT++ +  + FT  ++++GD WI   GVPY +G+     AAA
Sbjct: 541 WAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAAA 573

BLAST of Bhi02G001706 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 534.6 bits (1376), Expect = 9.7e-152
Identity = 274/577 (47.49%), Postives = 379/577 (65.68%), Query Frame = 0

Query: 4   GDDDAQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQGETSDPAIGSKSHEISASVKAI 63
           G D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I  +  A+
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAV 61

Query: 64  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTL-KEIEKD 123
           +A+C PTDYK+ CV SL  A  +S+ P +L++ GF   ++ I+ +  K SV L  +   D
Sbjct: 62  EAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAND 121

Query: 124 PRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDG 183
             T  AL  C++LM+ A D+LK  ++    F I ++++ + D+++WLS +I YQ+TC+D 
Sbjct: 122 KDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDT 181

Query: 184 FENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIP 243
           FE T    ++ M+K  KTS +L+SNGLAM++ IS++L E  + G++       R+LL   
Sbjct: 182 FEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS-- 241

Query: 244 VLGHDDYPDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVI 303
               D  P W  P  RRL+A    VK NVVVA DGSGQ++TI EAL+ VPK  N   +VI
Sbjct: 242 --AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVI 301

Query: 304 HIKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDG-TPTFKTATVAVTAEHFMA 363
           +IKQGVY E + + K +TH+  IGDGP KT ITGS N+  G   T+ TATVA+  ++F A
Sbjct: 302 YIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTA 361

Query: 364 RDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDF 423
           ++IGFENTAGP  HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCT+SGT+DF
Sbjct: 362 KNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDF 421

Query: 424 VFGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQ 483
           +FGD   V Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QN     +    PV+  
Sbjct: 422 IFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI 481

Query: 484 FRSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKR 543
            +++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y EY N GPGS++++R
Sbjct: 482 NKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQR 541

Query: 544 VKWRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYV 570
           VKW GIK ++P+ A+ FT  RFLRG+ WI    VPY+
Sbjct: 542 VKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Bhi02G001706 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-143
Identity = 263/577 (45.58%), Postives = 371/577 (64.30%), Query Frame = 0

Query: 7   DAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTLK-EIEKDPRTSK 126
           PTD+K  CV SL  A  +S DP +L++ GFK  +K I  +  K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  A+D+LK  M+    F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS +LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAA----GTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQF+TI +AL+ VPK  N   ++IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK+G+YKE + + K + H+  IGDGP KT+ITGS NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFV 426
           +IG ENTAGP   QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQF 486
           FGDA  + Q+C  +VRKP   Q C+VTA GR   R+ + L++       D    P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ LKT +Y E+ N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVS 571
           KW GIK +TPQ A+ +T  RFLRGD+WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Bhi02G001706 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 466.5 bits (1199), Expect = 3.2e-131
Identity = 268/704 (38.07%), Postives = 377/704 (53.55%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGS--KSHEISASVKAIKAICQPTDY 72
           +Y ++GV+     A+++A+ + V  N G +    +    +  ++  +   +  IC  TDY
Sbjct: 3   KYVLLGVT-----ALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDY 62

Query: 73  KQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKS-VTLK-EIEKDPRTSKALAG 132
           KQ+C  SL  A   S DP+ L+++ F  A+  I++  ++  + LK   + D  T +AL  
Sbjct: 63  KQDCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNT 122

Query: 133 CKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAA 192
           C+ELMD A+D+L+ + +K   F  ++L + + D+ +WLS +ITYQ+TC+DGFE     AA
Sbjct: 123 CRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAA 182

Query: 193 EKMKKALKTSMKLSSNGLAMVSQISSMLTELQIP-GISRRRLLEIPVLG----------- 252
             M++ ++    L+SNGLA+ + +  +L   +IP    R R   + +LG           
Sbjct: 183 VMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGS 242

Query: 253 ------------------------------------------------------------ 312
                                                                       
Sbjct: 243 SQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKK 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 SAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDS 362

Query: 373 --------HDDYPDWANPGMRRLLAAGTR---VKPNVVVAKDGSGQFRTIQEALDKVPKR 432
                     ++P W  P  RRLLA   R   +K NVVVAKDGSG+ +TI +AL  VP  
Sbjct: 363 LKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-M 422

Query: 433 KNNATYVIHIKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFI-DGTPTFKTATVA 492
           KN   +VIHIK+GVYKE + + K + H+M +GDGP KT+ITG   F+ D   T++TA+VA
Sbjct: 423 KNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVA 482

Query: 493 VTAEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC 552
           V  ++FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C
Sbjct: 483 VNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNC 542

Query: 553 TISGTIDFVFGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADA 569
            +SGTIDFVFGDA AVFQ+C F++R+P+ +QQCIVTA GRK+RR+ + ++I NS    DA
Sbjct: 543 RVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDA 602

BLAST of Bhi02G001706 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 2.0e-186
Identity = 312/575 (54.26%), Postives = 432/575 (75.13%), Query Frame = 0

Query: 1   MSYG--DDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASV 60
           MSYG  D+DA+ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI+ SV
Sbjct: 1   MSYGYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSV 60

Query: 61  KAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEK 120
           KAIK +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS T+ E++K
Sbjct: 61  KAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQK 120

Query: 121 DPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLD 180
           DPR   AL  CKELMD+A+ EL  S  +LG+F+  K+DE L+ ++IWLSATI++++TCLD
Sbjct: 121 DPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLD 180

Query: 181 GFENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHD 240
           GF+ T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        
Sbjct: 181 GFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------Q 240

Query: 241 DYPDWANPGMRRLL-AAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQ 300
           ++P W +   RRLL A  + VKP++VVA+DGSGQ++TI EAL+ VPK+K N T+V+HIK+
Sbjct: 241 EFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKE 300

Query: 301 GVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGF 360
           G+YKEY+ + +S+THL+ IGDGP KT+I+GSK++ DG  T+KTATVA+  +HF+A++I F
Sbjct: 301 GIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAF 360

Query: 361 ENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDA 420
           ENTAG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDA
Sbjct: 361 ENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDA 420

Query: 421 AAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFL 480
           AAVFQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q  +   + D   V++Q +++L
Sbjct: 421 AAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYL 480

Query: 481 GRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRG 540
           GRPWKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W G
Sbjct: 481 GRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPG 540

Query: 541 IKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGM 573
           IK ++ +  + FT  ++++GD+WI   GVPY+ G+
Sbjct: 541 IKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Bhi02G001706 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 652.1 bits (1681), Expect = 5.8e-186
Identity = 316/586 (53.92%), Postives = 437/586 (74.57%), Query Frame = 0

Query: 1   MSYG-DDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ--GETSDPAIGSKSHEISAS 60
           MSYG DD+++ K+RY +I +SS+LL++MVVAVTVGV+LN+  G++   A      E++AS
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA------EVNAS 60

Query: 61  VKAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIE 120
           VKA+K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS T+ E++
Sbjct: 61  VKAVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQ 120

Query: 121 KDPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCL 180
           KD RT  AL  CKELMD+A+DEL  S  +LG+F+   LDE LI+++IWLSA I+++ETCL
Sbjct: 121 KDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCL 180

Query: 181 DGFENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGH 240
           +GF+ T GNA E MKKALKT+++L+ NGLA++S++S+ + ++QIPG++ RRLL       
Sbjct: 181 EGFQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------ 240

Query: 241 DDYPDWANPGMRRLL---AAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIH 300
           + +P W +   R+LL   AA + VKP++VVA+DGSGQ++TI EAL  VPK K N T+V+H
Sbjct: 241 EGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVH 300

Query: 301 IKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARD 360
           IK G+YKEY+ + K+++HL+ IGDGP KTII+G+KN+ DG  T++TATVA+   +F+A++
Sbjct: 301 IKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKN 360

Query: 361 IGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVF 420
           IGFENTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCTISGTIDF+F
Sbjct: 361 IGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLF 420

Query: 421 GDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFR 480
           GDAAAVFQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q  +   + D   V++  +
Sbjct: 421 GDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSK 480

Query: 481 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVK 540
           ++LGRPWKEYSRTIIM ++I D +QP+GW PW GD+GLKT FY+E  N GPGS  + RV 
Sbjct: 481 AYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVT 540

Query: 541 WRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           W GIKT++ +  + FT  ++++GD WI   GVPY +G+     AAA
Sbjct: 541 WAGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAAA 573

BLAST of Bhi02G001706 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.4e-150
Identity = 274/577 (47.49%), Postives = 379/577 (65.68%), Query Frame = 0

Query: 4   GDDDAQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQGETSDPAIGSKSHEISASVKAI 63
           G D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I  +  A+
Sbjct: 2   GVDGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAV 61

Query: 64  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTL-KEIEKD 123
           +A+C PTDYK+ CV SL  A  +S+ P +L++ GF   ++ I+ +  K SV L  +   D
Sbjct: 62  EAVCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAAND 121

Query: 124 PRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDG 183
             T  AL  C++LM+ A D+LK  ++    F I ++++ + D+++WLS +I YQ+TC+D 
Sbjct: 122 KDTKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDT 181

Query: 184 FENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIP 243
           FE T    ++ M+K  KTS +L+SNGLAM++ IS++L E  + G++       R+LL   
Sbjct: 182 FEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS-- 241

Query: 244 VLGHDDYPDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVI 303
               D  P W  P  RRL+A    VK NVVVA DGSGQ++TI EAL+ VPK  N   +VI
Sbjct: 242 --AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVI 301

Query: 304 HIKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDG-TPTFKTATVAVTAEHFMA 363
           +IKQGVY E + + K +TH+  IGDGP KT ITGS N+  G   T+ TATVA+  ++F A
Sbjct: 302 YIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTA 361

Query: 364 RDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDF 423
           ++IGFENTAGP  HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCT+SGT+DF
Sbjct: 362 KNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDF 421

Query: 424 VFGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQ 483
           +FGD   V Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QN     +    PV+  
Sbjct: 422 IFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSI 481

Query: 484 FRSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKR 543
            +++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y EY N GPGS++++R
Sbjct: 482 NKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQR 541

Query: 544 VKWRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYV 570
           VKW GIK ++P+ A+ FT  RFLRG+ WI    VPY+
Sbjct: 542 VKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Bhi02G001706 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 507.7 bits (1306), Expect = 1.8e-142
Identity = 263/577 (45.58%), Postives = 371/577 (64.30%), Query Frame = 0

Query: 7   DAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTLK-EIEKDPRTSK 126
           PTD+K  CV SL  A  +S DP +L++ GFK  +K I  +  K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  A+D+LK  M+    F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS +LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAA----GTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQF+TI +AL+ VPK  N   ++IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK+G+YKE + + K + H+  IGDGP KT+ITGS NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFV 426
           +IG ENTAGP   QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQF 486
           FGDA  + Q+C  +VRKP   Q C+VTA GR   R+ + L++       D    P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ LKT +Y E+ N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPQHAVDFTAGRFLRGDSWIKSTGVPYVS 571
           KW GIK +TPQ A+ +T  RFLRGD+WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Bhi02G001706 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.5e-130
Identity = 268/704 (38.07%), Postives = 377/704 (53.55%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGS--KSHEISASVKAIKAICQPTDY 72
           +Y ++GV+     A+++A+ + V  N G +    +    +  ++  +   +  IC  TDY
Sbjct: 3   KYVLLGVT-----ALIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDY 62

Query: 73  KQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKS-VTLK-EIEKDPRTSKALAG 132
           KQ+C  SL  A   S DP+ L+++ F  A+  I++  ++  + LK   + D  T +AL  
Sbjct: 63  KQDCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNT 122

Query: 133 CKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAA 192
           C+ELMD A+D+L+ + +K   F  ++L + + D+ +WLS +ITYQ+TC+DGFE     AA
Sbjct: 123 CRELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAA 182

Query: 193 EKMKKALKTSMKLSSNGLAMVSQISSMLTELQIP-GISRRRLLEIPVLG----------- 252
             M++ ++    L+SNGLA+ + +  +L   +IP    R R   + +LG           
Sbjct: 183 VMMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGS 242

Query: 253 ------------------------------------------------------------ 312
                                                                       
Sbjct: 243 SQDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKK 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 SAFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDS 362

Query: 373 --------HDDYPDWANPGMRRLLAAGTR---VKPNVVVAKDGSGQFRTIQEALDKVPKR 432
                     ++P W  P  RRLLA   R   +K NVVVAKDGSG+ +TI +AL  VP  
Sbjct: 363 LKDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-M 422

Query: 433 KNNATYVIHIKQGVYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFI-DGTPTFKTATVA 492
           KN   +VIHIK+GVYKE + + K + H+M +GDGP KT+ITG   F+ D   T++TA+VA
Sbjct: 423 KNTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVA 482

Query: 493 VTAEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDC 552
           V  ++FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C
Sbjct: 483 VNGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNC 542

Query: 553 TISGTIDFVFGDAAAVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADA 569
            +SGTIDFVFGDA AVFQ+C F++R+P+ +QQCIVTA GRK+RR+ + ++I NS    DA
Sbjct: 543 RVSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDA 602

BLAST of Bhi02G001706 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY
Sbjct: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV
Sbjct: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH 587
           ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH
Sbjct: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH 586

BLAST of Bhi02G001706 vs. NCBI nr
Match: XP_008450602.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >KAA0050920.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa] >TYK10268.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 560/580 (96.55%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAG+RVKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           ITPQHA+DFTAGRF+ GD WIK TGVPYVSGMTRT  AAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAA 580

BLAST of Bhi02G001706 vs. NCBI nr
Match: XP_004135775.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.1 hypothetical protein Csa_007353 [Cucumis sativus])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 532/580 (91.72%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQECVASLKA GNNSSDPKELVQAGFKAAMKLIQAAANKSV L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA+DELKYSMNKLGEFDISKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQF+TIQEA+D+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+++KK+LTHLMLIGDGPKKTIITG+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFK  ADL PVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           ITPQHAVDFT GRFL+GD WIK TGVPYVSG+TRT  A A
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGA 580

BLAST of Bhi02G001706 vs. NCBI nr
Match: XP_022931680.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 978.8 bits (2529), Expect = 2.1e-281
Identity = 480/578 (83.04%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQ  TSDPA G K+HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KSVTL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALA CKELMD++VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-TRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGV 300
           P+W +PGM+RLLA    + KPN+VVAKDGSGQ++TIQEA++KVPKRKNNAT+VIHIKQGV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY+ I+K LTHLMLIGDGPKKTIITG+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGR 480
           VFQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FK DADL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 TITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRA 578
           +ITPQHAVDFT GRF+ GD WIK+TGVPYVSGMT+ +A
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKA 575

BLAST of Bhi02G001706 vs. NCBI nr
Match: XP_022969472.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 978.4 bits (2528), Expect = 2.7e-281
Identity = 480/581 (82.62%), Postives = 534/581 (91.91%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQ  TSDPA G K+HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KS+TL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALAGCKELMDF+VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            N+TG+A+EKMKKALK SM+LSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-TRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGV 300
           P+WA PGM+RLLA    + KPN+VVAKDGSGQ++TIQEA++KVPKRKNNAT+VIHIKQGV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY++I K LTHLMLIGDGPKKTII+G+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGR 480
           VFQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FK DADL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGL+TCFYTE+NNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 TITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           +IT QHAVDFT GRF+ GD WIK+TG+PYVSGMT+  A  A
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVA 578

BLAST of Bhi02G001706 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 560/580 (96.55%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAG+RVKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           ITPQHA+DFTAGRF+ GD WIK TGVPYVSGMTRT  AAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAA 580

BLAST of Bhi02G001706 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 560/580 (96.55%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAG+RVKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN S KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           ITPQHA+DFTAGRF+ GD WIK TGVPYVSGMTRT  AAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAA 580

BLAST of Bhi02G001706 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 532/580 (91.72%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETSDPA G+KSHEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAICQPTDYKQECVASLKA GNNSSDPKELVQAGFKAAMKLIQAAANKSV L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA+DELKYSMNKLGEFDISKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQF+TIQEA+D+VPKRKNNATYVIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+++KK+LTHLMLIGDGPKKTIITG+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFK  ADL PVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           ITPQHAVDFT GRFL+GD WIK TGVPYVSG+TRT  A A
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGA 580

BLAST of Bhi02G001706 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.0e-281
Identity = 480/578 (83.04%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQ  TSDPA G K+HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KSVTL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALA CKELMD++VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-TRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGV 300
           P+W +PGM+RLLA    + KPN+VVAKDGSGQ++TIQEA++KVPKRKNNAT+VIHIKQGV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY+ I+K LTHLMLIGDGPKKTIITG+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGR 480
           VFQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FK DADL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTE+NN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 TITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRA 578
           +ITPQHAVDFT GRF+ GD WIK+TGVPYVSGMT+ +A
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTKPKA 575

BLAST of Bhi02G001706 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.3e-281
Identity = 480/581 (82.62%), Postives = 534/581 (91.91%), Query Frame = 0

Query: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60
           M+Y D  A+N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQ  TSDPA G K+HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KS+TL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALAGCKELMDF+VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            N+TG+A+EKMKKALK SM+LSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-TRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGV 300
           P+WA PGM+RLLA    + KPN+VVAKDGSGQ++TIQEA++KVPKRKNNAT+VIHIKQGV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY++I K LTHLMLIGDGPKKTII+G+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGR 480
           VFQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQNS FK DADL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGL+TCFYTE+NNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 TITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAA 581
           +IT QHAVDFT GRF+ GD WIK+TG+PYVSGMT+  A  A
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVA 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G27870.11.4e-18754.26Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.14.2e-18753.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.19.7e-15247.49Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.11.3e-14345.58Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.13.2e-13138.07Plant invertase/pectin methylesterase inhibitor superfamily [more]
Match NameE-valueIdentityDescription
Q3E8Z82.0e-18654.26Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX865.8e-18653.92Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ211.4e-15047.49Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA11.8e-14245.58Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
O234474.5e-13038.07Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_038880188.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida][more]
XP_008450602.10.0e+0092.24PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >K... [more]
XP_004135775.10.0e+0091.72putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.... [more]
XP_022931680.12.1e-28183.04putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata][more]
XP_022969472.12.7e-28182.62putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5D3CGC30.0e+0092.24Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180... [more]
A0A1S3BPI80.0e+0092.24Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1[more]
A0A0A0LYF50.0e+0091.72Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1[more]
A0A6J1F0581.0e-28183.04Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1[more]
A0A6J1HWF11.3e-28182.62Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 56..208
e-value: 1.9E-36
score: 137.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 60..208
e-value: 3.0E-26
score: 92.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 55..212
e-value: 1.9E-23
score: 80.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 261..558
e-value: 8.8E-140
score: 464.6
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 255..572
e-value: 1.5E-120
score: 404.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 56..213
e-value: 1.1E-33
score: 118.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 59..213
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 16..575
NoneNo IPR availablePANTHERPTHR31707:SF131PECTINESTERASEcoord: 16..575
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 63..214
e-value: 3.2185E-44
score: 152.592
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 406..415
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 287..306
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 256..571

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001706Bhi02M001706mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity