Bhi02G001689 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001689
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionIsoaspartyl peptidase/L-asparaginase
Locationchr2: 55346874 .. 55350550 (+)
RNA-Seq ExpressionBhi02G001689
SyntenyBhi02G001689
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAGACAAACTTCATATTTCGATCGAGCCAAACAGAAATGACAGTAAAACGTTGAATAGCAGTACACTATAAAATTTCCTGACCGAAGCAGAATGGAGAAGTTGCACCAACTCCGCTACTAAATTCACAATCCTTCTCCATTAATACACTCTCGCCAATCGCCGGCGAATGACCTGGATAATCCTTTAGCGATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTGAGAAATTTTTACCTTTTTTTTTTTTTTTTTCTTTCACTCTGTCTTGATATGACTTTTAGTTCCATGAGTTTTAGAATCTAATGATTTAGTTTAGTGTGTGTAATCTTCAAGTTTGTACTGAATTTGTTTACTGGTTTTAGTATGTAATGATATAGTTCAAGATGCAGTTTAAATTTATAATGATTTAGTCTCTAATGTGAGAAAATTTGATTAGGTGTAATGGAGTTTCGTAATTAGATTGATGGATGAATTGCTTAGAGACCTAATAAAGTTTTCATGGATTTTAATCCGGGGATTAAATTCTTATCTTCCTGAGGATTAAATCAATATAAATATTAAATCACAATGACTTTCTTTTCTGTCTCCCTTTCTTTCTACTTGCTGAAACGCAGCTAATCTGGGTCCTTGCTTTTCTGCTAATGGGAGGTTGCGTGTGCTTTCATTTGGTTTATTAAGTACTTTCTTGTGATGATTCCCTTACTGATAAGTTAATGGCTTTGGGGATTTGAGTTTTGATGTTATATACAAATTATGCCCTTTTAAATTTTAAGGCTAAATGCACTTTTGGTCCCTGAGGTTTAGAGTAAGTGTCTATTTGGTCCTTAAGATTTCAAAACATACATTTTAGTCCTTGGGGTTTGAAAAATGGTCCTTGAGTTAATTTGACCGTTAGTTAACTAATGTAAATTTGACGTGCCAAGTTGAGATGACATATTTTAAATAAGGTGGATAACTTTCCTAAGTTTATATTATTTTTAGGTCACATGGATTTTTTAAGTATTTTTTTCCCTCTCCGCCCTAAAAATTTTGACAAAAATATATTTTCAGCTCATTCGTTGATGGCTGTTAAGCATGGAAATGGATCATTGATGGAAATTGGAGAACTTATTGTTAGCAATTTCTTAGGGGGTGTTTGTCCTATGGATTAATCAGTCATCAGTTGTGATAGTTACAAAAATTTGAATTGTGTTAGTTCTGGGAATATATTATTTGTAAAAGACAATATAGACTTAGGTGAGTTTCATAAGTTTATATTTGATGGTCTAGAAAGATCATATGAACTAAGGATAATATCTTTTATTTTAGTGACCTTTAGCTTTAGTTTACATAACTTGAAATTTATGTTATATGTGGGATCAGCAGTCTCTATAGTTGTAAATAAGTAAACCTATATGTACTAACATAAGTTTTAATCAACTCTCTAGATTACACAAATTTAATTTGTGAGTCAAACATGTCCTGATAATTTAAAACTCATTTGATGGTTTAGATTATCTAAACTTAGGGTGAACATCTTCTGTTCTAATCACTTTTACTTTCAAGTCTGACCCTTTTGTTTGAACATTTTGTTTGGTAACCCGAATTCATAGTTGTTGCCCCTAATCAAGATCTAATAAGTTTCATTGTAGTATAAATCATTTTGACTAGATAATCAGTTCTTTCTTATCCATGGCTTCTTCTCCTTGTTAAGATGATTAGTTTTTTTTTTTTTTTTNNNNTTTTTTTTTTTTTTTTTTTTTTTTTCTTGTACAGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGTAAGCAATTTCTATCCCTCTTCCTATGTAGCTGCAGAATCAATCCTCTCTTTACATATTATTTTGGCACTAATACAGATAGATTCATTGTGATTCATGACTCTTTTATTTCTCAAAAAATGTCGAGGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGGTTTTCTGCTTACCTTTTATCCCAATACATGAATTAACTGCTCTCTTTGTCTCTGAACTTACCTGCATATTATATTGTTAAGCTAGAATTATGTCAACGTCTAGGCATTTTCACAATATGTTAAATATTGATGTTGATAAAAATGTCGAGATTTTAATATTATAAAAATTAAGAGGAAATATCAATAAAATGTCAGTGTTAGATGGATATTTCTAAGGAATTTATAAAAGAAAATGTGCGAGTTAGAGAAATTGGTAATCCACGGTTTTTAAAATAAGTTAAAAGTTCTTAATGTCGTTTAATCTTGTTTAAATTTTTAATATTTTGTTGGTGCGTAAGGTATAACAGAAATATTGATTCACCATATACGTCGATGTTGAACCTATAAATATATGACAATGTTGATGAAAATATTGATATGTTGACGAAAATTTCATGCCTCGGTTGCTAAAACTCTTCATAGATCAGAAGTTTCTGATTATATATATAGGTTATAGATCTAGCTGTGCATCCAATAAGGTTGAAAAAGACGCATTACGGAGTGTTTTTTTTATAGCATCTGGCGTTTTTTATTTTTTATGGTACTTTCTTGTCTGCACAGTGATAGCCCTCTGTTTTGAACCCTTGTAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGAATGAATATTGTGACAAATCCAGAGCTGAAATTTGGTTCTATTCTGATGTTTTGATGGTTGGTGTCTCTCAGCTTTTGTTGTTTTTCCTAAATATTAAGAATAGTCTTCCAAATGTTCTTCCATTTCATGTATTTGGTTCAATTTCAATGGCAAGATATTCTCAATTATTAGTTAAACATAAGTACAAGATATTTTCTCCTCCCTACATATATACTTAAAATTTTCTACCCA

mRNA sequence

ATTGAGACAAACTTCATATTTCGATCGAGCCAAACAGAAATGACAGTAAAACGTTGAATAGCAGTACACTATAAAATTTCCTGACCGAAGCAGAATGGAGAAGTTGCACCAACTCCGCTACTAAATTCACAATCCTTCTCCATTAATACACTCTCGCCAATCGCCGGCGAATGACCTGGATAATCCTTTAGCGATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGAATGAATATTGTGACAAATCCAGAGCTGAAATTTGGTTCTATTCTGATGTTTTGATGGTTGGTGTCTCTCAGCTTTTGTTGTTTTTCCTAAATATTAAGAATAGTCTTCCAAATGTTCTTCCATTTCATGTATTTGGTTCAATTTCAATGGCAAGATATTCTCAATTATTAGTTAAACATAAGTACAAGATATTTTCTCCTCCCTACATATATACTTAAAATTTTCTACCCA

Coding sequence (CDS)

ATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGA

Protein sequence

MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEIAIWSSKND
Homology
[Close]
BLAST of Bhi02G001689 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 488.4 bits (1256), Expect = 4.3e-138
Identity = 254/316 (80.38%), Postives = 275/316 (87.03%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP  LP ERR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GT+EMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFD AEAFAR  GVET +SSHFITP+NI RLKQAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGM 300
           E IIRGTVARDVAALME+KGLSL EA AYVV +SVP+G+ GLVAVSA+GEV +P+NTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIW 315
           FRACA+EDGYSEIAIW
Sbjct: 302 FRACASEDGYSEIAIW 312

[Close]
BLAST of Bhi02G001689 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 367.1 bits (941), Expect = 1.5e-101
Identity = 195/237 (82.28%), Postives = 210/237 (88.61%), Query Frame = 0 

Query: 80  MEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETTNS 139
           MEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFD AEAFAR  GVET +S
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 SHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANGDSQIGTVGCVAVDNHGNL 199
           SHFITP+NI RLKQAKE NRVQ+DYT P P+  +       GDSQIGTVGCVAVD+ GNL
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----CGDSQIGTVGCVAVDSAGNL 120

Query: 200 ASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVAALMEFK 259
           ASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKGE IIRGTVARDVAALME+K
Sbjct: 121 ASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVAALMEYK 180

Query: 260 GLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEIAIW 315
           GLSL EA AYVV +SVP+G+ GLVAVSA+GEV +P+NTTGMFRACA+EDGYSEIAIW
Sbjct: 181 GLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEIAIW 232

[Close]
BLAST of Bhi02G001689 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.3e-98
Identity = 190/323 (58.82%), Postives = 238/323 (73.68%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GWAIA+HGGAG I  +LP ER++  ++ L RCL +G+ AL+S    +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAG-IDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMDSTK-RCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
           P FN+GRGS LT KGT+EMEASIMD TK RCGAVSG+TTV NPISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPL-----PQATEQET 181
           F GAE FAR+QGVE  ++ +F+T  N+  LK AKEAN +  DY  P        AT+   
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 ENANGDSQI---GTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
           +       I    TVGCV VD  G+ A+ TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 ISATGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVVE-SVPKGNVGLVAVSASGEVAI 301
           +S TG+GEAIIR T+ARDV+A+ME+KGL+L+EA  YV++  + +G  GL+AVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

Query: 302 PYNTTGMFRACATEDGYSEIAIW 315
            +N+ GMFR CATEDG+ E+AIW
Sbjct: 303 GFNSNGMFRGCATEDGFMEVAIW 324

[Close]
BLAST of Bhi02G001689 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 7.6e-18
Identity = 87/335 (25.97%), Postives = 140/335 (41.79%), Query Frame = 0 

Query: 5   IALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHF 64
           +A+H GAG   +      +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVME----------K 124
           NAGRGS LT  G +E +AS+MD  +   G V  +  V N I +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFAREQ-----GVETTNSSHFITPQNIERLKQAK----EANRVQIDYT 184
            P + L  +GA  + + +     G  T      +T +   + ++ K    E     I   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 QPLPQATEQE---TEN------ANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRI 244
           +  P+ TE      EN      A+ D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAISATGKGEAIIRGTVARDVAALMEFKGLSLEEATA 293
           G     G+G +A+         L     +G GE ++RG  AR+    +         A  
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

[Close]
BLAST of Bhi02G001689 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 7.1e-16
Identity = 85/326 (26.07%), Postives = 137/326 (42.02%), Query Frame = 0 

Query: 5   IALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHF 64
           +A+H GAG   +      +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPIS--LARLVMEKTPHIYLAFD 124
           NAGRGS LT  G +E +AS+MD     G   G+  V + +S  L++L++       L  +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS--GMFGGVGAVPDQVSEMLSKLLL----CWLLVGE 134

Query: 125 GAEAFAREQ-----GVETTNSSHFITPQNIERLKQAK----EANRVQIDYTQPLPQATEQ 184
           GA  + + +     G  T      +T +   + ++ K    E     I   +  P+ TE 
Sbjct: 135 GARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTEN 194

Query: 185 E---TEN------ANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAG 244
                EN      A+ D  + TVG + VDN G++A  +S+GG+  K+ GR+G     G+G
Sbjct: 195 NETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSG 254

Query: 245 TYAN--------HLCAISATGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVVESV--- 293
            +A+         L     +G GE ++RG  AR+    +         A   V+ SV   
Sbjct: 255 CWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRSVMHQ 314

[Close]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.9e-138
Identity = 256/320 (80.00%), Postives = 280/320 (87.50%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERRQPREE LR CLQIGV+ALKSQKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD  T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYT-----QPLPQATEQET 181
           F GA+ FA++QGVET +SSHFIT +N+ERLK A EANRVQ+DY+     QP     E+E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
             ANGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGEAII  TVARDVAALMEFKGLSL+EA  YVV E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRA ATEDGYSEIAIW
Sbjct: 303 TTGMFRASATEDGYSEIAIW 322

[Close]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.6e-137
Identity = 251/320 (78.44%), Postives = 281/320 (87.81%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERR+PREE LR CLQIGV+ALK+QKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD +T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQ-----PLPQATEQET 181
           F GA+ FA++QGVET +SSH IT +N+ERLK A EANRVQ+DY+Q     P+    E+E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
              NGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGE IIR TVARDVAALMEFKGLSL+EA  +V+ E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRACATEDGYSEIAIW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

[Close]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 488.4 bits (1256), Expect = 6.1e-137
Identity = 254/316 (80.38%), Postives = 275/316 (87.03%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP  LP ERR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GT+EMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFD AEAFAR  GVET +SSHFITP+NI RLKQAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGM 300
           E IIRGTVARDVAALME+KGLSL EA AYVV +SVP+G+ GLVAVSA+GEV +P+NTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIW 315
           FRACA+EDGYSEIAIW
Sbjct: 302 FRACASEDGYSEIAIW 312

[Close]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 3.9e-136
Identity = 250/320 (78.12%), Postives = 278/320 (86.88%), Query Frame = 0 

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERR+PREE LR CLQIG++ALK+Q PPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD +T +CGAVSGL TV+NPISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQID-----YTQPLPQATEQET 181
           F GA+   ++QGVET +SSHFIT +N+ERLK A EANRVQ+D     YTQP+    E+E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
             ANGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGEAIIR TVARDVAALMEFKGLSL+EA   VV E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRACATEDGYSEIAIW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

[Close]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match: P30362 (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 237/303 (78.22%), Postives = 264/303 (87.13%), Query Frame = 0 

Query: 19  PPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHFNAGRGSVLTTKGTI 78
           PPERR+PREE LR CLQIGV+ALK++K PLDVVELVVRELEN  HFNAG GSVLT  GT+
Sbjct: 1   PPERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTV 60

Query: 79  EMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETTN 138
           EMEASIMD  + +CGAVSGL+TV+NPISLARLVMEKTPH+YLAF GA+ FA++QGVET +
Sbjct: 61  EMEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVD 120

Query: 139 SSHFITPQNIERLKQAKEANRVQID-----YTQPLPQATEQETENANGDSQIGTVGCVAV 198
           SSHFIT +N+ERLK A EANRVQID     YTQP+    E+E   ANGDSQIGTVGCVAV
Sbjct: 121 SSHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAV 180

Query: 199 DNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVA 258
           D+ GNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SATGKGEAII+ TVARDVA
Sbjct: 181 DSQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVA 240

Query: 259 ALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEI 315
           ALMEFKGLSL+EA  YVV E  PKG VGL+AVSA+GE+A+P+NTTGMFRACATEDG SEI
Sbjct: 241 ALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEI 300

[Close]
BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_038878291.1 (isoaspartyl peptidase/L-asparaginase isoform X1 [Benincasa hispida])

HSP 1 Score: 629.0 bits (1621), Expect = 2.2e-176
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG
Sbjct: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEIAIWSSKND
Sbjct: 301 ACATEDGYSEIAIWSSKND 319

[Close]
BLAST of Bhi02G001689 vs. NCBI nr
Match: KAA0050881.1 (isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa])

HSP 1 Score: 594.3 bits (1531), Expect = 6.0e-166
Identity = 300/319 (94.04%), Postives = 312/319 (97.81%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_008450657.1 (PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo] >TYK10233.1 isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa])

HSP 1 Score: 592.4 bits (1526), Expect = 2.3e-165
Identity = 299/319 (93.73%), Postives = 311/319 (97.49%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_038878292.1 (isoaspartyl peptidase/L-asparaginase isoform X2 [Benincasa hispida])

HSP 1 Score: 590.5 bits (1521), Expect = 8.7e-165
Identity = 305/319 (95.61%), Postives = 305/319 (95.61%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFARE              QNIERLKQAKEANRVQIDYTQPLPQATEQETENANG
Sbjct: 121 FDGAEAFARE--------------QNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEIAIWSSKND
Sbjct: 301 ACATEDGYSEIAIWSSKND 305

[Close]
BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_004135623.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 hypothetical protein Csa_007301 [Cucumis sativus])

HSP 1 Score: 587.0 bits (1512), Expect = 9.6e-164
Identity = 298/319 (93.42%), Postives = 309/319 (96.87%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK T+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN +G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           +IIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match: A0A5A7UBC6 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00060 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.9e-166
Identity = 300/319 (94.04%), Postives = 312/319 (97.81%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match: A0A5D3CEE4 (Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003800 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 299/319 (93.73%), Postives = 311/319 (97.49%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match: A0A1S3BPR4 (isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 299/319 (93.73%), Postives = 311/319 (97.49%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match: A0A0A0LW19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.6e-164
Identity = 298/319 (93.42%), Postives = 309/319 (96.87%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK T+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN +G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           +IIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

[Close]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match: A0A6J1F0F1 (isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC111441019 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.5e-157
Identity = 287/316 (90.82%), Postives = 297/316 (93.99%), Query Frame = 0 

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SL  +RRQPREETLRRCLQIGVQALK QKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GT+EMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVETT+ S+FITPQNIERLKQAKEAN VQIDYTQP PQAT QETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFK LSLEEA A  VESVPKGNVGL+AVSASG+VA+P+NTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSS 317
           ACATEDGYSEIAIW S
Sbjct: 301 ACATEDGYSEIAIWPS 316

The following BLAST results are available for this feature:
BLAST of Bhi02G001689 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G08100.14.3e-13880.38N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT5G08100.21.5e-10182.28N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT3G16150.13.3e-9858.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.17.6e-1825.97N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G00590.27.1e-1626.07N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
BLAST of Bhi02G001689 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P303641.9e-13880.00Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
Q9ZSD61.6e-13778.44Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
P502876.1e-13780.38Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
P502883.9e-13678.13Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
P303621.1e-12578.22Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2... [more]
BLAST of Bhi02G001689 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038878291.12.2e-176100.00isoaspartyl peptidase/L-asparaginase isoform X1 [Benincasa hispida][more]
KAA0050881.16.0e-16694.04isoaspartyl peptidase/L-asparaginase [Cucumis melo var. makuwa][more]
XP_008450657.12.3e-16593.73PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo] >TYK10233.1 isoas... [more]
XP_038878292.18.7e-16595.61isoaspartyl peptidase/L-asparaginase isoform X2 [Benincasa hispida][more]
XP_004135623.19.6e-16493.42isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 hypothetica... [more]
BLAST of Bhi02G001689 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UBC62.9e-16694.04Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CEE41.1e-16593.73Isoaspartyl peptidase/L-asparaginase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BPR41.1e-16593.73isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=... [more]
A0A0A0LW194.6e-16493.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1[more]
A0A6J1F0F15.5e-15790.82isoaspartyl peptidase/L-asparaginase 1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 3..314
e-value: 8.7E-111
score: 369.9
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..314
NoneNo IPR availableGENE3D3.60.20.30(Glycosyl)asparaginasecoord: 186..317
e-value: 5.4E-43
score: 148.3
NoneNo IPR availablePIRSRPIRSR600246-2PIRSR600246-2coord: 2..318
e-value: 7.3E-107
score: 354.8
NoneNo IPR availablePIRSRPIRSR600246-3PIRSR600246-3coord: 2..318
e-value: 7.3E-107
score: 354.8
NoneNo IPR availablePIRSRPIRSR600246-1PIRSR600246-1coord: 2..318
e-value: 7.3E-107
score: 354.8
NoneNo IPR availablePANTHERPTHR10188:SF33ISOASPARTYL PEPTIDASE/L-ASPARAGINASEcoord: 1..314
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 4..307
e-value: 2.35489E-134
score: 380.268
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 3..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001689Bhi02M001689mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0008146 sulfotransferase activity
molecular_function GO:0016787 hydrolase activity