Bhi02G001681 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001681
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionheavy metal-associated isoprenylated plant protein 34
Locationchr2: 55036425 .. 55039251 (+)
RNA-Seq ExpressionBhi02G001681
SyntenyBhi02G001681
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTCTGTTTCTTCTTCTTCTCTCTTTCAAAATATTCCATTCCATTTCTCTTTCTCTTTCTTCAATTTTTCTTCTCAACACTTTTTCTTTTTCCCCATTTCCCCATTTCCCAATTCTTTCTTCTTCTTCTTCTTCTAAATCTTGAAGAGAAGAGAAGAAAATTTTGCAACACAGAGACACACCCCACTTTGTGAAGAAGCTTCAATTACAAGAACACCTTAAAAGTGAAATCCCCTCAAAACCCACTTTCATTTTTGTCTCTCACAAGACACAAAAACCTTCCAACACTTTATTCTCTCTAACACACTATGAGTAAACAGGATTTCATGAACATTCAGGTACATTTGAGGAATTTTTATTATTATTTTGTTTGTTCCCTTGATTCTTGTGTTTGCCCTCAATTTAAGGAACTGTTTGGTTTAAAATCTTGGTGGGTTTTTAGTGAAATTGATGTTTGTTTACAGGCTTGTGCCCTTAGAGTTAACATTCATTGTGATGGATGCAAACAGAAGGTCAAGAAAATTCTTCAGAAAATTGATGGTAAGTGGTAATCATTAGGGTTTTTTTTTTGTTTCTCTCCCTGATTTAACCAAACTAGAACTCAAACCCTTGATTAGTTCAGAGGTTTGTTACTTTTTTTGTGTTTTTGGAGAGATTTTTTACTGTTTTCTGTTGTGGGTTTTTTTTTTTTTTTTTTTGTTTGTTTGTTTGTTTTTGTTGTTTTAGGAGTTTACACTGTGAAAATAGATGCAGAACAAGGGAAAGTGACAGTAACAGGAAACATAGATCCAGGGAAACTGATAAAGAAGCTTGAGAAATCAGGGAAACATGCTGAGTTATGGGGAGCTCAGATAAGTTCAAACAACAATATGAACAATTTCAACAATCAGTTTAAGCAGATGCAGTTTCAGAATGTCAAAGGAGGAGTAGGAGGAAAAGACAACAATATGAACAACCACAAGTTTCAAATGGAGAAAGCTTTTCTTCAACATCATCAACAACAACAGCAACAACAATTGAAGGGCTCTAACAAAGGAGGAGGTAATAACAACAATAATATGCATAGTCTTATGGTTCCTCCTAAAGATAAGAAGTCTGTTAAGTTCAATTTGCCTGAGGAAGATTCTGATTTTAGTGATGATGAGTTTGATGATGAGTTTGATGATGAATTTGATGATGATGATGAGTTTGATGATGAAGATGATGAGTTTAGCCATGGACATGGACATGGAAATGGACATGGGCATGGCTTACCTAACAATATGATGCCTGTGATGGGTAAAAAAGGAGGGTCATTTGGGCCTAATGAGTCCAATGGAATGATTAATTTCAATGCTATGAATGGTAAGAAAGGTGGGGATGGAGGTACTGCCAAGAAAGGTGAAGTTATTGACTTTCCTGTTATGATGAAGGGTAAGGGAGGAGGAGGGGGTAATGAAGGTAAAACCAACAAGGGAGGAGGGAAGAAAGACGGTAGCGGTAGCGATAGCGGCAGTGGGACGAAGAGTAAGAGCAAAGGAGGGGATCAGAAGAAAGGGGGGAAGGATAAAAATGGAGGGAAGAAAGGGGGTGGATTTCTAGGACTAGGTAAAAAGGGGCTGTTTGGTTTTGGTGGTAAAAATGGTAGCAAGAAAGACAGTGGTAGTAAGAAGAACAAAGGGAGCAGCAATCATAAAAGTGCTGAGAATGGTGGAGGAAAACATAATGGCAATGGGGCCAAAAAAGGAGGGAAAAATGATGGCCTCCATGAGTTTGACAAGTTAGATATTGGCTTCCATGACCTTGATATTACCAAACCAAGCAAAGGAGGAAAAGGAAGTGGTGGAAATGGAGGTGGTAATGGTAAGAATATGGGCCAAATGGGCCAAATGGGTCATGCTAATGGAAACAAAGGACAGATGGGTCATATGGGACAGATGGGCCCGATGGGCCAGATGGGCCATGGTGGTGGCAATAGGGGCCCACCAATGGGAAATTATGCAATGAATCAGATGGGTAACATTCCAGCAGTACAAGGTCTACCAGCTCAACCAATGATGAATGGAGGTGGAGGATATTATCAAGGAATGGGACAAGGGAATCCTTACAGCCAACAACAACAACAGTACATGGCTATGATGATGAACCAACAAAGGGGAGTTGGGAATGATATGTACCAACACATGATGTATTCGCGCCCGAACCCAGCCGTGAACTACGCTCCACCGCCCCCGATGTTCCCTCCAGTCGCAACCGATCACTACACGCATTTCTTCAGTGACGAGAATACCGATAGTTGCAGTATCATGTAAATTTTGCCCTTTCCTTTGGTTTATTTTCTAGGATTAGTGTTTTTGTGGTAGTTTATATAGCTTTAGCTGAATGTAGAAAATGGGTGCAAGGCTTTTATAGTAGTACCATTGCTTGTGTTACTTGAGAAAAAAAAAAAGAAGAAAAAAATGGGATCAAAACTTTGATTCTTTACTGTTTAGTACCTCAAAAACCCA

mRNA sequence

CACTCTGTTTCTTCTTCTTCTCTCTTTCAAAATATTCCATTCCATTTCTCTTTCTCTTTCTTCAATTTTTCTTCTCAACACTTTTTCTTTTTCCCCATTTCCCCATTTCCCAATTCTTTCTTCTTCTTCTTCTTCTAAATCTTGAAGAGAAGAGAAGAAAATTTTGCAACACAGAGACACACCCCACTTTGTGAAGAAGCTTCAATTACAAGAACACCTTAAAAGTGAAATCCCCTCAAAACCCACTTTCATTTTTGTCTCTCACAAGACACAAAAACCTTCCAACACTTTATTCTCTCTAACACACTATGAGTAAACAGGATTTCATGAACATTCAGGCTTGTGCCCTTAGAGTTAACATTCATTGTGATGGATGCAAACAGAAGGTCAAGAAAATTCTTCAGAAAATTGATGGAGTTTACACTGTGAAAATAGATGCAGAACAAGGGAAAGTGACAGTAACAGGAAACATAGATCCAGGGAAACTGATAAAGAAGCTTGAGAAATCAGGGAAACATGCTGAGTTATGGGGAGCTCAGATAAGTTCAAACAACAATATGAACAATTTCAACAATCAGTTTAAGCAGATGCAGTTTCAGAATGTCAAAGGAGGAGTAGGAGGAAAAGACAACAATATGAACAACCACAAGTTTCAAATGGAGAAAGCTTTTCTTCAACATCATCAACAACAACAGCAACAACAATTGAAGGGCTCTAACAAAGGAGGAGGTAATAACAACAATAATATGCATAGTCTTATGGTTCCTCCTAAAGATAAGAAGTCTGTTAAGTTCAATTTGCCTGAGGAAGATTCTGATTTTAGTGATGATGAGTTTGATGATGAGTTTGATGATGAATTTGATGATGATGATGAGTTTGATGATGAAGATGATGAGTTTAGCCATGGACATGGACATGGAAATGGACATGGGCATGGCTTACCTAACAATATGATGCCTGTGATGGGTAAAAAAGGAGGGTCATTTGGGCCTAATGAGTCCAATGGAATGATTAATTTCAATGCTATGAATGGTAAGAAAGGTGGGGATGGAGGTACTGCCAAGAAAGACAGTGGTAGTAAGAAGAACAAAGGGAGCAGCAATCATAAAAGTGCTGAGAATGGTGGAGGAAAACATAATGGCAATGGGGCCAAAAAAGGAGGGAAAAATGATGGCCTCCATGAGTTTGACAAGTTAGATATTGGCTTCCATGACCTTGATATTACCAAACCAAGCAAAGGAGGAAAAGGAAGTGGTGGAAATGGAGGTGGTAATGGTAAGAATATGGGCCAAATGGGCCAAATGGGTCATGCTAATGGAAACAAAGGACAGATGGGTCATATGGGACAGATGGGCCCGATGGGCCAGATGGGCCATGGTGGTGGCAATAGGGGCCCACCAATGGGAAATTATGCAATGAATCAGATGGGTAACATTCCAGCAGTACAAGGTCTACCAGCTCAACCAATGATGAATGGAGGTGGAGGATATTATCAAGGAATGGGACAAGGGAATCCTTACAGCCAACAACAACAACAGTACATGGCTATGATGATGAACCAACAAAGGGGAGTTGGGAATGATATGTACCAACACATGATGTATTCGCGCCCGAACCCAGCCGTGAACTACGCTCCACCGCCCCCGATGTTCCCTCCAGTCGCAACCGATCACTACACGCATTTCTTCAGTGACGAGAATACCGATAGTTGCAGTATCATGTAAATTTTGCCCTTTCCTTTGGTTTATTTTCTAGGATTAGTGTTTTTGTGGTAGTTTATATAGCTTTAGCTGAATGTAGAAAATGGGTGCAAGGCTTTTATAGTAGTACCATTGCTTGTGTTACTTGAGAAAAAAAAAAAGAAGAAAAAAATGGGATCAAAACTTTGATTCTTTACTGTTTAGTACCTCAAAAACCCA

Coding sequence (CDS)

ATGAGTAAACAGGATTTCATGAACATTCAGGCTTGTGCCCTTAGAGTTAACATTCATTGTGATGGATGCAAACAGAAGGTCAAGAAAATTCTTCAGAAAATTGATGGAGTTTACACTGTGAAAATAGATGCAGAACAAGGGAAAGTGACAGTAACAGGAAACATAGATCCAGGGAAACTGATAAAGAAGCTTGAGAAATCAGGGAAACATGCTGAGTTATGGGGAGCTCAGATAAGTTCAAACAACAATATGAACAATTTCAACAATCAGTTTAAGCAGATGCAGTTTCAGAATGTCAAAGGAGGAGTAGGAGGAAAAGACAACAATATGAACAACCACAAGTTTCAAATGGAGAAAGCTTTTCTTCAACATCATCAACAACAACAGCAACAACAATTGAAGGGCTCTAACAAAGGAGGAGGTAATAACAACAATAATATGCATAGTCTTATGGTTCCTCCTAAAGATAAGAAGTCTGTTAAGTTCAATTTGCCTGAGGAAGATTCTGATTTTAGTGATGATGAGTTTGATGATGAGTTTGATGATGAATTTGATGATGATGATGAGTTTGATGATGAAGATGATGAGTTTAGCCATGGACATGGACATGGAAATGGACATGGGCATGGCTTACCTAACAATATGATGCCTGTGATGGGTAAAAAAGGAGGGTCATTTGGGCCTAATGAGTCCAATGGAATGATTAATTTCAATGCTATGAATGGTAAGAAAGGTGGGGATGGAGGTACTGCCAAGAAAGACAGTGGTAGTAAGAAGAACAAAGGGAGCAGCAATCATAAAAGTGCTGAGAATGGTGGAGGAAAACATAATGGCAATGGGGCCAAAAAAGGAGGGAAAAATGATGGCCTCCATGAGTTTGACAAGTTAGATATTGGCTTCCATGACCTTGATATTACCAAACCAAGCAAAGGAGGAAAAGGAAGTGGTGGAAATGGAGGTGGTAATGGTAAGAATATGGGCCAAATGGGCCAAATGGGTCATGCTAATGGAAACAAAGGACAGATGGGTCATATGGGACAGATGGGCCCGATGGGCCAGATGGGCCATGGTGGTGGCAATAGGGGCCCACCAATGGGAAATTATGCAATGAATCAGATGGGTAACATTCCAGCAGTACAAGGTCTACCAGCTCAACCAATGATGAATGGAGGTGGAGGATATTATCAAGGAATGGGACAAGGGAATCCTTACAGCCAACAACAACAACAGTACATGGCTATGATGATGAACCAACAAAGGGGAGTTGGGAATGATATGTACCAACACATGATGTATTCGCGCCCGAACCCAGCCGTGAACTACGCTCCACCGCCCCCGATGTTCCCTCCAGTCGCAACCGATCACTACACGCATTTCTTCAGTGACGAGAATACCGATAGTTGCAGTATCATGTAA

Protein sequence

MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKLIKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKAFLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGDGGTAKKDSGSKKNKGSSNHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMMNGGGGYYQGMGQGNPYSQQQQQYMAMMMNQQRGVGNDMYQHMMYSRPNPAVNYAPPPPMFPPVATDHYTHFFSDENTDSCSIM
Homology
BLAST of Bhi02G001681 vs. TAIR 10
Match: AT3G05220.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 4.1e-68
Identity = 243/590 (41.19%), Postives = 285/590 (48.31%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           M+KQD M +Q C L+VN+HC+GCK KVKK LQKI+GVY+VK D EQG+VTVTGNIDP  L
Sbjct: 1   MNKQDVMKLQTCVLKVNVHCEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGNIDPALL 60

Query: 61  IKKLEKSGKHAEL----WGAQISSNNNMNNFNNQFKQM------------QFQNVKGGVG 120
           +KKL KSGKHAE+     G          N N QF  +            +   VKG  G
Sbjct: 61  VKKLSKSGKHAEILGGGGGGGGGGGKGFPNLNGQFANLNMGGNNKPKGGKESNQVKGKAG 120

Query: 121 GKDNNMNNHKFQMEKAFLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNL 180
           G      NH    +   L   Q QQ   +K ++ GGG     M   M     KKSVKF  
Sbjct: 121 GGGGGGQNHG-HGQPMQLNPQQIQQMMMMKAAHGGGGGGQMKMPP-MAAKDQKKSVKF-A 180

Query: 181 PEEDSDFSDDEFDDE-FDDEFDDDDEFD---DEDDEFSHGHGHGNGHGHG-------LPN 240
            +ED +FS+D++DDE F ++  DDDEFD   D+DDE  HGHGHG G G+        +PN
Sbjct: 181 DDEDDEFSEDDYDDEDFSEDDYDDDEFDDDEDDDDEMGHGHGHGGGGGNHMPPNKMMMPN 240

Query: 241 NMMPVM----GKKGGSFGPNESNGMIN-----------------------------FNAM 300
            MMP M    G  GG  GPNE   M+N                              N  
Sbjct: 241 KMMPQMGGHHGNGGGPKGPNEIMMMMNGFKGGGGGGKKGGGGGFEIPVQMKGMGEGKNGK 300

Query: 301 NGKKGGDGGTAKKDSGSKKNKGSSNHKSAENGGG------------------------KH 360
           +GKKG  G   KK+    K  G +    A++GGG                         H
Sbjct: 301 DGKKGKGGEKGKKEGKENKGGGKTGKTDAKSGGGGLLGFFKKGKSGNGDEKKSAGKKDGH 360

Query: 361 NGNGAKKGGKNDGLHEFDK--------LDIGFH---DLD-ITKPSKGGKGSGGNGGGNGK 420
            GN  K  G   G+  +D            G H   D+D + K SKGG G G  G  N  
Sbjct: 361 GGNKVKSHGGGGGVQHYDSGPKKGGGGTKGGGHGGLDIDELMKHSKGGGGGGNKGNHNHS 420

Query: 421 NMGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGG-----GNRGP--PMGNYAMNQMGNIP 472
             G  G      G  G  G MGQ GPMG MG GG     G  GP  PMG     Q G+ P
Sbjct: 421 AKGIGGGPMGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGPMGPMG----GQGGSYP 480

BLAST of Bhi02G001681 vs. TAIR 10
Match: AT5G19090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.8e-66
Identity = 254/614 (41.37%), Postives = 295/614 (48.05%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KIDAE GKVTV+GN+DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLEKSGKHAELWGA-QISSNNNMN--NFNNQFKQMQFQN-VKGGVGGKDNNMNNHK-- 120
           IKKL KSGKHAE+WGA +  SNNN N  N  NQFK MQ  +  KGG GG     NN+K  
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 ------------------------------FQMEKAFLQHHQQ-QQQQQLKGSN------ 180
                                          QM++   Q  QQ QQ QQ+KG        
Sbjct: 121 KIGGGGGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPP 180

Query: 181 --KGG-----GNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEFD-DDDE 240
             KGG     G+   N +  M    ++K+VKFN+P++D    +++F DEFDDEFD DDDE
Sbjct: 181 QLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDD---DEEDFSDEFDDEFDEDDDE 240

Query: 241 FDD--EDDEFSHGHGHGN-------GHGHGLPNNMMPVM-----------GKKGGSFGPN 300
           FDD  EDDEF       N       G    +PNNMMP M            K GG  GP 
Sbjct: 241 FDDDLEDDEFDDHPPPPNKMKPMMGGGNMIMPNNMMPNMMMPNAQQMLNAHKNGGGPGPA 300

Query: 301 ----ESNGMINFNAMNGKKGGDGGTAKKDSGSKKNKGSSNHKSAENGGGKHNGNGAKKGG 360
               E  GM     M G  GG GG      G   N G+ N            G G K GG
Sbjct: 301 GGKIEGKGMPFPVQMGGGGGGPGGKKGGPGGGGGNMGNQN-----------QGGGGKNGG 360

Query: 361 KNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHANGNKGQMGHM 420
           K  G H  D            K   GG G  GN GG G  M      G   G  G  G  
Sbjct: 361 KGGGGHPLD-----------GKMGGGGGGPNGNKGGGGVQMNGGPNGGKKGGGGGGGGGG 420

Query: 421 GQMG--------PMGQMGHGGGNRGP-----PMGNYAMNQMGNIP--------------A 472
           G M         PMG  G GGG  GP     PMG      MG++P              A
Sbjct: 421 GPMSGGLPPGFRPMG--GGGGGGGGPQSMSMPMGGAMGGPMGSLPQMGGGPGPMSNNMQA 480

BLAST of Bhi02G001681 vs. TAIR 10
Match: AT3G06130.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 7.2e-65
Identity = 224/522 (42.91%), Postives = 265/522 (50.77%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KID+EQGKVTV+G++DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVL 60

Query: 61  IKKLEKSGKHAELWGAQISSNN-NMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQM-- 120
           IKKL KSGKHAE+WGA   +NN N +   NQFK MQ  + K G GG  NN NN K Q   
Sbjct: 61  IKKLAKSGKHAEIWGAPKGNNNPNQSQMANQFKGMQIDHGKAGGGGGGNNNNNKKGQKNG 120

Query: 121 ------------------EKAFLQHHQQ-QQQQQLKGSN--------KGGGNNNNNMHSL 180
                             ++   QH QQ QQ Q++KG          KG    N N    
Sbjct: 121 GGGGGGGGGGNSNAPKMGQQLNPQHLQQLQQLQKMKGFQDLKLPPQLKGSVPVNKN---- 180

Query: 181 MVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEF--DDDDEFDDEDDEFSHGHGHGNGHG 240
               +++K VKF++PE+D    DD+F DEFDDEF  DDDDEFDDE D+            
Sbjct: 181 ----QNQKGVKFDVPEDD---DDDDFSDEFDDEFTDDDDDEFDDEFDDLP---------- 240

Query: 241 HGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGDGGTAKKDSGSKKNKGSSNHKS 300
             LP+N M    K   +  PN    M+N        GG      K + +    G+   K 
Sbjct: 241 --LPSNKM----KPNMTMMPNAQQMMMNAQKNANLAGGPAKNGGKGAPAAGGGGAGGGKG 300

Query: 301 AENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQ 360
           A  G     GN  + GGKN G         G H  D       GK  GG GG N    G 
Sbjct: 301 AGGGAKGGPGNQNQGGGKNGG---------GGHPQD-------GKNGGGGGGPNAGKKGN 360

Query: 361 MGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMM 420
            G    A G  G    MG  GP       GG  G  MG      MGN+PAVQGLPA    
Sbjct: 361 GGGGPMAGGVSGGFRPMGGGGPQNMSMPMGGQMG--MG----GPMGNMPAVQGLPATGPG 420

Query: 421 NGGGGYYQGMG---QGNPYSQQQQQYMAMMMNQQRGVGNDMYQHMMYSRPNPAVNYAPPP 472
               GY+QG G         QQQQQY+A +MNQQR +GN+ +Q MMY+RP PAVNY PP 
Sbjct: 421 GAPQGYFQGAGIDPMQMQQQQQQQQYLAAVMNQQRAMGNERFQPMMYARPPPAVNYMPPN 473

BLAST of Bhi02G001681 vs. TAIR 10
Match: AT5G19090.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 2.1e-56
Identity = 222/565 (39.29%), Postives = 266/565 (47.08%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KIDAE GKVTV+GN+DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLEKSGKHAELWGA-QISSNNNMN--NFNNQFKQMQFQN-VKGGVGGKDNNMNNHK-- 120
           IKKL KSGKHAE+WGA +  SNNN N  N  NQFK MQ  +  KGG GG     NN+K  
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 ------------------------------FQMEKAFLQHHQQ-QQQQQLKGSN------ 180
                                          QM++   Q  QQ QQ QQ+KG        
Sbjct: 121 KIGGGGGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPP 180

Query: 181 --KGG-----GNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEFD-DDDE 240
             KGG     G+   N +  M    ++K+VKFN+P++D    +++F DEFDDEFD DDDE
Sbjct: 181 QLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDD---DEEDFSDEFDDEFDEDDDE 240

Query: 241 FDD--EDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGD 300
           FDD  EDDEF       + H    PN M P+MG  G    PN  N M N    N +    
Sbjct: 241 FDDDLEDDEF-------DDHPPP-PNKMKPMMG-GGNMIMPN--NMMPNMMMPNAQ---- 300

Query: 301 GGTAKKDSGSKKNKGSSNHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITK 360
                                                                      +
Sbjct: 301 -----------------------------------------------------------Q 360

Query: 361 PSKGGKGSGGNGGGNGKNMGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGN 420
                K  GG GG    +M   G MG            G MG + QMG G G    PM N
Sbjct: 361 MLNAHKNGGGGGGPQSMSMPMGGAMG------------GPMGSLPQMGGGPG----PMSN 420

Query: 421 YAMNQMGNIPAVQGLPAQPMMNGGG---------GYYQGM-------GQ----GNPY--- 472
                  N+ AVQGLPA     GGG         GY+QG        GQ    GNPY   
Sbjct: 421 -------NMQAVQGLPAMGPGGGGGGGPSAEAPPGYFQGQVSGNGGGGQDSMPGNPYLQQ 465

BLAST of Bhi02G001681 vs. TAIR 10
Match: AT5G19090.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 2.1e-56
Identity = 222/565 (39.29%), Postives = 266/565 (47.08%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KIDAE GKVTV+GN+DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLEKSGKHAELWGA-QISSNNNMN--NFNNQFKQMQFQN-VKGGVGGKDNNMNNHK-- 120
           IKKL KSGKHAE+WGA +  SNNN N  N  NQFK MQ  +  KGG GG     NN+K  
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 ------------------------------FQMEKAFLQHHQQ-QQQQQLKGSN------ 180
                                          QM++   Q  QQ QQ QQ+KG        
Sbjct: 121 KIGGGGGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPP 180

Query: 181 --KGG-----GNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEFD-DDDE 240
             KGG     G+   N +  M    ++K+VKFN+P++D    +++F DEFDDEFD DDDE
Sbjct: 181 QLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDD---DEEDFSDEFDDEFDEDDDE 240

Query: 241 FDD--EDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGD 300
           FDD  EDDEF       + H    PN M P+MG  G    PN  N M N    N +    
Sbjct: 241 FDDDLEDDEF-------DDHPPP-PNKMKPMMG-GGNMIMPN--NMMPNMMMPNAQ---- 300

Query: 301 GGTAKKDSGSKKNKGSSNHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITK 360
                                                                      +
Sbjct: 301 -----------------------------------------------------------Q 360

Query: 361 PSKGGKGSGGNGGGNGKNMGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGN 420
                K  GG GG    +M   G MG            G MG + QMG G G    PM N
Sbjct: 361 MLNAHKNGGGGGGPQSMSMPMGGAMG------------GPMGSLPQMGGGPG----PMSN 420

Query: 421 YAMNQMGNIPAVQGLPAQPMMNGGG---------GYYQGM-------GQ----GNPY--- 472
                  N+ AVQGLPA     GGG         GY+QG        GQ    GNPY   
Sbjct: 421 -------NMQAVQGLPAMGPGGGGGGGPSAEAPPGYFQGQVSGNGGGGQDSMPGNPYLQQ 465

BLAST of Bhi02G001681 vs. ExPASy Swiss-Prot
Match: Q0WV37 (Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX=3702 GN=HIPP34 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.7e-67
Identity = 243/590 (41.19%), Postives = 285/590 (48.31%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           M+KQD M +Q C L+VN+HC+GCK KVKK LQKI+GVY+VK D EQG+VTVTGNIDP  L
Sbjct: 1   MNKQDVMKLQTCVLKVNVHCEGCKHKVKKQLQKIEGVYSVKADVEQGRVTVTGNIDPALL 60

Query: 61  IKKLEKSGKHAEL----WGAQISSNNNMNNFNNQFKQM------------QFQNVKGGVG 120
           +KKL KSGKHAE+     G          N N QF  +            +   VKG  G
Sbjct: 61  VKKLSKSGKHAEILGGGGGGGGGGGKGFPNLNGQFANLNMGGNNKPKGGKESNQVKGKAG 120

Query: 121 GKDNNMNNHKFQMEKAFLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNL 180
           G      NH    +   L   Q QQ   +K ++ GGG     M   M     KKSVKF  
Sbjct: 121 GGGGGGQNHG-HGQPMQLNPQQIQQMMMMKAAHGGGGGGQMKMPP-MAAKDQKKSVKF-A 180

Query: 181 PEEDSDFSDDEFDDE-FDDEFDDDDEFD---DEDDEFSHGHGHGNGHGHG-------LPN 240
            +ED +FS+D++DDE F ++  DDDEFD   D+DDE  HGHGHG G G+        +PN
Sbjct: 181 DDEDDEFSEDDYDDEDFSEDDYDDDEFDDDEDDDDEMGHGHGHGGGGGNHMPPNKMMMPN 240

Query: 241 NMMPVM----GKKGGSFGPNESNGMIN-----------------------------FNAM 300
            MMP M    G  GG  GPNE   M+N                              N  
Sbjct: 241 KMMPQMGGHHGNGGGPKGPNEIMMMMNGFKGGGGGGKKGGGGGFEIPVQMKGMGEGKNGK 300

Query: 301 NGKKGGDGGTAKKDSGSKKNKGSSNHKSAENGGG------------------------KH 360
           +GKKG  G   KK+    K  G +    A++GGG                         H
Sbjct: 301 DGKKGKGGEKGKKEGKENKGGGKTGKTDAKSGGGGLLGFFKKGKSGNGDEKKSAGKKDGH 360

Query: 361 NGNGAKKGGKNDGLHEFDK--------LDIGFH---DLD-ITKPSKGGKGSGGNGGGNGK 420
            GN  K  G   G+  +D            G H   D+D + K SKGG G G  G  N  
Sbjct: 361 GGNKVKSHGGGGGVQHYDSGPKKGGGGTKGGGHGGLDIDELMKHSKGGGGGGNKGNHNHS 420

Query: 421 NMGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGG-----GNRGP--PMGNYAMNQMGNIP 472
             G  G      G  G  G MGQ GPMG MG GG     G  GP  PMG     Q G+ P
Sbjct: 421 AKGIGGGPMGPGGPMGPGGPMGQGGPMGMMGPGGPMSMMGPGGPMGPMG----GQGGSYP 480

BLAST of Bhi02G001681 vs. ExPASy Swiss-Prot
Match: F4JZL7 (Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX=3702 GN=HIPP33 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.4e-65
Identity = 254/614 (41.37%), Postives = 295/614 (48.05%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KIDAE GKVTV+GN+DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDAELGKVTVSGNVDPSVL 60

Query: 61  IKKLEKSGKHAELWGA-QISSNNNMN--NFNNQFKQMQFQN-VKGGVGGKDNNMNNHK-- 120
           IKKL KSGKHAE+WGA +  SNNN N  N  NQFK MQ  +  KGG GG     NN+K  
Sbjct: 61  IKKLLKSGKHAEIWGAPKGGSNNNQNQPNLANQFKAMQIDHGGKGGGGGGGGPANNNKGQ 120

Query: 121 ------------------------------FQMEKAFLQHHQQ-QQQQQLKGSN------ 180
                                          QM++   Q  QQ QQ QQ+KG        
Sbjct: 121 KIGGGGGGGGGGGGGGGGGPPKMVIPQLTPQQMQQLNPQQLQQLQQLQQMKGFQDLKLPP 180

Query: 181 --KGG-----GNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEFD-DDDE 240
             KGG     G+   N +  M    ++K+VKFN+P++D    +++F DEFDDEFD DDDE
Sbjct: 181 QLKGGPGPGPGSVPMNKNPQMPNNPNQKAVKFNVPDDD---DEEDFSDEFDDEFDEDDDE 240

Query: 241 FDD--EDDEFSHGHGHGN-------GHGHGLPNNMMPVM-----------GKKGGSFGPN 300
           FDD  EDDEF       N       G    +PNNMMP M            K GG  GP 
Sbjct: 241 FDDDLEDDEFDDHPPPPNKMKPMMGGGNMIMPNNMMPNMMMPNAQQMLNAHKNGGGPGPA 300

Query: 301 ----ESNGMINFNAMNGKKGGDGGTAKKDSGSKKNKGSSNHKSAENGGGKHNGNGAKKGG 360
               E  GM     M G  GG GG      G   N G+ N            G G K GG
Sbjct: 301 GGKIEGKGMPFPVQMGGGGGGPGGKKGGPGGGGGNMGNQN-----------QGGGGKNGG 360

Query: 361 KNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHANGNKGQMGHM 420
           K  G H  D            K   GG G  GN GG G  M      G   G  G  G  
Sbjct: 361 KGGGGHPLD-----------GKMGGGGGGPNGNKGGGGVQMNGGPNGGKKGGGGGGGGGG 420

Query: 421 GQMG--------PMGQMGHGGGNRGP-----PMGNYAMNQMGNIP--------------A 472
           G M         PMG  G GGG  GP     PMG      MG++P              A
Sbjct: 421 GPMSGGLPPGFRPMG--GGGGGGGGPQSMSMPMGGAMGGPMGSLPQMGGGPGPMSNNMQA 480

BLAST of Bhi02G001681 vs. ExPASy Swiss-Prot
Match: Q9M8K5 (Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX=3702 GN=HIPP32 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.0e-63
Identity = 224/522 (42.91%), Postives = 265/522 (50.77%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSK++FM IQ C L+VNIHCDGCKQKVKKILQKI+GV+T KID+EQGKVTV+G++DP  L
Sbjct: 1   MSKEEFMKIQTCVLKVNIHCDGCKQKVKKILQKIEGVFTTKIDSEQGKVTVSGSVDPSVL 60

Query: 61  IKKLEKSGKHAELWGAQISSNN-NMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQM-- 120
           IKKL KSGKHAE+WGA   +NN N +   NQFK MQ  + K G GG  NN NN K Q   
Sbjct: 61  IKKLAKSGKHAEIWGAPKGNNNPNQSQMANQFKGMQIDHGKAGGGGGGNNNNNKKGQKNG 120

Query: 121 ------------------EKAFLQHHQQ-QQQQQLKGSN--------KGGGNNNNNMHSL 180
                             ++   QH QQ QQ Q++KG          KG    N N    
Sbjct: 121 GGGGGGGGGGNSNAPKMGQQLNPQHLQQLQQLQKMKGFQDLKLPPQLKGSVPVNKN---- 180

Query: 181 MVPPKDKKSVKFNLPEEDSDFSDDEFDDEFDDEF--DDDDEFDDEDDEFSHGHGHGNGHG 240
               +++K VKF++PE+D    DD+F DEFDDEF  DDDDEFDDE D+            
Sbjct: 181 ----QNQKGVKFDVPEDD---DDDDFSDEFDDEFTDDDDDEFDDEFDDLP---------- 240

Query: 241 HGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGDGGTAKKDSGSKKNKGSSNHKS 300
             LP+N M    K   +  PN    M+N        GG      K + +    G+   K 
Sbjct: 241 --LPSNKM----KPNMTMMPNAQQMMMNAQKNANLAGGPAKNGGKGAPAAGGGGAGGGKG 300

Query: 301 AENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQ 360
           A  G     GN  + GGKN G         G H  D       GK  GG GG N    G 
Sbjct: 301 AGGGAKGGPGNQNQGGGKNGG---------GGHPQD-------GKNGGGGGGPNAGKKGN 360

Query: 361 MGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMM 420
            G    A G  G    MG  GP       GG  G  MG      MGN+PAVQGLPA    
Sbjct: 361 GGGGPMAGGVSGGFRPMGGGGPQNMSMPMGGQMG--MG----GPMGNMPAVQGLPATGPG 420

Query: 421 NGGGGYYQGMG---QGNPYSQQQQQYMAMMMNQQRGVGNDMYQHMMYSRPNPAVNYAPPP 472
               GY+QG G         QQQQQY+A +MNQQR +GN+ +Q MMY+RP PAVNY PP 
Sbjct: 421 GAPQGYFQGAGIDPMQMQQQQQQQQYLAAVMNQQRAMGNERFQPMMYARPPPAVNYMPPN 473

BLAST of Bhi02G001681 vs. ExPASy Swiss-Prot
Match: Q84J88 (Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX=3702 GN=HIPP36 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 5.3e-20
Identity = 55/102 (53.92%), Postives = 70/102 (68.63%), Query Frame = 0

Query: 14  LRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKLIKKLEKSGKHAEL 73
           LRV+IHC+GCK+K+KKIL KIDGVYT  ID +Q KVTV GN++P  LIKK+ K+G+HAEL
Sbjct: 34  LRVSIHCEGCKRKIKKILSKIDGVYTTNIDVKQQKVTVIGNVEPEILIKKIMKAGRHAEL 93

Query: 74  WGAQISSNNNMNNFNNQFKQMQFQN--VKGGVGGKDNNMNNH 114
           W    S  NN+NN  N  K+ +  N    G     +NN NN+
Sbjct: 94  W--PTSMENNINNDCNYQKKPKKDNEETSGDEDDDENNNNNN 133

BLAST of Bhi02G001681 vs. ExPASy Swiss-Prot
Match: A2RVM8 (Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX=3702 GN=HIPP37 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.2e-18
Identity = 48/90 (53.33%), Postives = 62/90 (68.89%), Query Frame = 0

Query: 4  QDF--MNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKLI 63
          +DF  + IQ  +LRVNIHC+GC +KVKK+LQ+I+GV  VKI+AE  KVTV+G++D   LI
Sbjct: 5  EDFKLLKIQTFSLRVNIHCEGCNKKVKKLLQRIEGVCHVKIEAEHQKVTVSGSVDSATLI 64

Query: 64 KKLEKSGKHAELWGAQISSNNNMNNFNNQF 92
           KL K+GKHAELW    + N       N F
Sbjct: 65 NKLVKAGKHAELWSPNPNQNQPQKPKTNDF 94

BLAST of Bhi02G001681 vs. NCBI nr
Match: XP_038879083.1 (heavy metal-associated isoprenylated plant protein 34-like [Benincasa hispida])

HSP 1 Score: 878.2 bits (2268), Expect = 3.1e-251
Identity = 471/556 (84.71%), Postives = 471/556 (84.71%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKA 120
           IKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKA
Sbjct: 61  IKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKA 120

Query: 121 FLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEF 180
           FLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEF
Sbjct: 121 FLQHHQQQQQQQLKGSNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDEF 180

Query: 181 DDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAM 240
           DDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAM
Sbjct: 181 DDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAM 240

Query: 241 NGKKGGDGGTA------------------------------------------------- 300
           NGKKGGDGGTA                                                 
Sbjct: 241 NGKKGGDGGTAKKGEVIDFPVMMKGKGGGGGNEGKTNKGGGKKDGSGSDSGSGTKSKSKG 300

Query: 301 ------------------------------------KKDSGSKKNKGSSNHKSAENGGGK 360
                                               KKDSGSKKNKGSSNHKSAENGGGK
Sbjct: 301 GDQKKGGKDKNGGKKGGGFLGLGKKGLFGFGGKNGSKKDSGSKKNKGSSNHKSAENGGGK 360

Query: 361 HNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHA 420
           HNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHA
Sbjct: 361 HNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHA 420

Query: 421 NGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMMNGGGGYY 472
           NGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMMNGGGGYY
Sbjct: 421 NGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLPAQPMMNGGGGYY 480

BLAST of Bhi02G001681 vs. NCBI nr
Match: XP_008450667.1 (PREDICTED: cell wall protein IFF6-like [Cucumis melo] >TYK10226.1 cell wall protein IFF6-like [Cucumis melo var. makuwa])

HSP 1 Score: 699.1 bits (1803), Expect = 2.6e-197
Identity = 416/584 (71.23%), Postives = 435/584 (74.49%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQAC LRVNIHCDGCKQKVKKILQKI+GVYTVKID+EQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACVLRVNIHCDGCKQKVKKILQKIEGVYTVKIDSEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSN-----NNMNNFNNQFKQMQFQNVK-----GGVGGKDNNM 120
           IKKLEKSGKHAELWG QISSN     NN NNFNNQFKQMQFQNVK     GG GGKDNNM
Sbjct: 61  IKKLEKSGKHAELWGKQISSNNMNNFNNYNNFNNQFKQMQFQNVKGGGGGGGGGGKDNNM 120

Query: 121 NNHKFQMEKAFLQHHQQQQQQQLKGS----NKGGG-NNNNNMHSLMVPPKDKKSVKFNLP 180
           NNHKFQMEKA+LQ HQQQQQQQLKGS    NKGG   NNNNMHSLMVPPKDKKSVKFNLP
Sbjct: 121 NNHKFQMEKAYLQ-HQQQQQQQLKGSNNITNKGGNITNNNNMHSLMVPPKDKKSVKFNLP 180

Query: 181 EEDSDFSDDEF-DDEFDDEFDDD--DEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKK 240
           EE+SDFS DEF DDEFDD+FDDD  D+ DDEDDEFSHGHGH  GHGHGLPNNMMPVMGKK
Sbjct: 181 EEESDFSGDEFDDDEFDDDFDDDDYDDEDDEDDEFSHGHGH--GHGHGLPNNMMPVMGKK 240

Query: 241 GGSFGPNESNGMINFNAMNGKKGGDGGTA------------------------------- 300
           GGSFG NE NGM++FNAMNGKKGGDGG A                               
Sbjct: 241 GGSFGANEHNGMMSFNAMNGKKGGDGGNAKKGEVIDFPIMMKSKGGGNEGKAGKGGGKKD 300

Query: 301 ----------------------------------------------------KKDSGSKK 360
                                                               KKD+G KK
Sbjct: 301 GNSSGSDSGTKSKSKGGDQKKVGKDKNGGKKGGGFLGLGKKGLFGFGGKNGSKKDNGGKK 360

Query: 361 NKGSS-NHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSG-- 420
           NKGSS N+KS+ENGGGKHN NGAKKGGKNDGLHEFDK+D+GFHDLDI KPSKGGK +G  
Sbjct: 361 NKGSSNNNKSSENGGGKHNSNGAKKGGKNDGLHEFDKMDLGFHDLDIAKPSKGGKVNGGG 420

Query: 421 -----GNGGGNGKN---MGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNY 472
                GNG GN KN   M QMGQ+GH NGN+GQ         MG MGH G +RGPPMGNY
Sbjct: 421 NGNGNGNGNGNSKNMSQMNQMGQLGHGNGNRGQ---------MGPMGHAGVSRGPPMGNY 480

BLAST of Bhi02G001681 vs. NCBI nr
Match: XP_031741111.1 (heavy metal-associated isoprenylated plant protein 34 [Cucumis sativus] >KAE8653376.1 hypothetical protein Csa_007575 [Cucumis sativus])

HSP 1 Score: 677.9 bits (1748), Expect = 6.1e-191
Identity = 406/576 (70.49%), Postives = 429/576 (74.48%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQAC LRVNIHCDGCKQKV+KILQKI+GVYTVKID+EQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACVLRVNIHCDGCKQKVRKILQKIEGVYTVKIDSEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSN-----NNMNNFNNQFKQMQFQNVK--GGVGGKDNNMNNH 120
           IKKLEKSGKHAELWG QISSN     NN NNFNNQFKQMQFQNVK  GG GGKDNNMNNH
Sbjct: 61  IKKLEKSGKHAELWGKQISSNNMNNFNNYNNFNNQFKQMQFQNVKGGGGGGGKDNNMNNH 120

Query: 121 KFQMEKAFLQH-HQQQQQQQLKGS----NKGGG-NNNNNMHSLMVPPKDKKSVKFNLPEE 180
           K QMEKA+LQH  QQQQQQQLKGS    NKGG  +NNNNMHSLMVPPKDKKSVKFNLPEE
Sbjct: 121 KLQMEKAYLQHQQQQQQQQQLKGSNNITNKGGNISNNNNMHSLMVPPKDKKSVKFNLPEE 180

Query: 181 DSDFSDDEF-DDEFDDEFDDD--DEFDDEDDEFS--HGHGHGNGHGHGLPNNMMPVMGKK 240
           +SDFS DEF DDEFDDEFDDD  D+ DDEDD+FS  HGHGHG+GHGHGLPNNM+P MGKK
Sbjct: 181 ESDFSGDEFDDDEFDDEFDDDDYDDEDDEDDDFSHGHGHGHGHGHGHGLPNNMLPGMGKK 240

Query: 241 GGSFGPNESNGMINFNAMNGKKGGDGGTA------------------------------- 300
           GGSFG NE     NFN MNGKKGGDGG A                               
Sbjct: 241 GGSFGANEPVAYTNFNPMNGKKGGDGGYAKKGEVIDFPIVMKSKGGGNEGKGGKGGGKKD 300

Query: 301 ----------------------------------------------KKDSGSKKNKGSS- 360
                                                         KKD+G KKNKGSS 
Sbjct: 301 GNSSGSDSGTKSKSKGGDQKKGGKKGGGFLGLGKKGLFGFGGKNGSKKDNGGKKNKGSSN 360

Query: 361 NHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSG-GNGGGNG 420
           N+KS+ENGGGKHNGNGAKKGGK+DGLHEFDK+D+GFHDLDI KPSKGGKG+G GNGG NG
Sbjct: 361 NNKSSENGGGKHNGNGAKKGGKSDGLHEFDKMDLGFHDLDIAKPSKGGKGNGNGNGGSNG 420

Query: 421 KNMGQMGQMGHANGNKGQMGHMGQMGPMGQ-------MGHGGGNRGPPMGNYAMNQMGNI 472
              G     G++NGN   +  M QMG MG        MGH G +RGPPMGNYAMN MGNI
Sbjct: 421 N--GNSNGNGNSNGNSKNISQMNQMGQMGHGNGNRGPMGHAGVSRGPPMGNYAMNPMGNI 480

BLAST of Bhi02G001681 vs. NCBI nr
Match: XP_022159661.1 (heavy metal-associated isoprenylated plant protein 34 [Momordica charantia])

HSP 1 Score: 651.7 bits (1680), Expect = 4.7e-183
Identity = 392/571 (68.65%), Postives = 414/571 (72.50%), Query Frame = 0

Query: 2   SKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKLI 61
           +KQDFM IQACALRVNIHC+GCKQKVKKILQKIDGVYTVKIDAEQGKV+VTGNIDP KLI
Sbjct: 3   NKQDFMKIQACALRVNIHCEGCKQKVKKILQKIDGVYTVKIDAEQGKVSVTGNIDPAKLI 62

Query: 62  KKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKAF 121
           +KLEKSGKHAELWGAQ++  +N NN NNQFK MQFQNVKGG GGKDNNM N+KFQMEKAF
Sbjct: 63  QKLEKSGKHAELWGAQLNP-SNFNN-NNQFKSMQFQNVKGGGGGKDNNMMNNKFQMEKAF 122

Query: 122 LQHHQQQQQQQLKG--SNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDE 181
           LQ  QQQQQQQLKG  +NKGG +N NN+++LM PPKDKKSVKFNLPEE SDFS DEFDDE
Sbjct: 123 LQQQQQQQQQQLKGFSNNKGGNHNLNNLNNLMAPPKDKKSVKFNLPEEVSDFSGDEFDDE 182

Query: 182 FDDEFDD-DDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSF---GPNESNGMI 241
           FDDEFDD DDEFDDEDDE   GHGH  GHGH LPN MMP+MGK GGS    GP   NGMI
Sbjct: 183 FDDEFDDEDDEFDDEDDEQEFGHGH--GHGHALPNKMMPMMGKGGGSLGSNGPMGPNGMI 242

Query: 242 NFNAMNGKKGGDGGT--------------------------------------------- 301
           NFN MN KK GDG                                               
Sbjct: 243 NFNMMNSKKSGDGAAKKGEVIDFPVMMKGNKGGGGGNEGKNSKGGGKKDGGGGGGGGGGG 302

Query: 302 ---------------------------------------AKKDSGSKKNKGSSNHKSAE- 361
                                                   KK+SG  K KGSSN+KS+E 
Sbjct: 303 GGNKSKSKGGGEQKKEGKKGGGFLGLGKKGLFGFGGKNGGKKESGHSKKKGSSNNKSSEG 362

Query: 362 NGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMG 421
            GGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDI+KPSKGGKG+    GGNGK+MGQMG
Sbjct: 363 GGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDISKPSKGGKGN----GGNGKSMGQMG 422

Query: 422 QMGHANGNKGQMGHMGQMGPM-------GQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLP 472
           QMGH N NK QMG MGQMG M       GQMG GG N GPPMGNY M QMGNIPAVQGLP
Sbjct: 423 QMGHGNANKSQMGQMGQMGQMGPGGINRGQMGPGGANMGPPMGNYPMTQMGNIPAVQGLP 482

BLAST of Bhi02G001681 vs. NCBI nr
Match: XP_022961512.1 (heavy metal-associated isoprenylated plant protein 34-like [Cucurbita moschata] >KAG7023942.1 Heavy metal-associated isoprenylated plant protein 32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.4 bits (1513), Expect = 1.1e-163
Identity = 381/549 (69.40%), Postives = 399/549 (72.68%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKA 120
           IKKLEKSGKHAELWGAQISS NNMNNFNN      FQNVK   GGKDNNM+NHK  MEKA
Sbjct: 61  IKKLEKSGKHAELWGAQISS-NNMNNFNN-----LFQNVK---GGKDNNMDNHKLLMEKA 120

Query: 121 FLQHHQQQQQQQLKGS----NKGGGNN--NNNMHSLMVPPKDKKSVKFNLPEEDSDFSDD 180
           FL     QQQQQ+KGS    NKGGGNN  NNNMHS++VP KDKKSVKFNLPEEDS+FS D
Sbjct: 121 FL-----QQQQQMKGSNNGMNKGGGNNNHNNNMHSVVVPGKDKKSVKFNLPEEDSEFS-D 180

Query: 181 EFDDEFDDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKK-GGSFGPNESNG 240
           EFDDEFDDEF  DDEFDDE DEFSH       HGHGLPNNMMP+MG K GGSFG   +NG
Sbjct: 181 EFDDEFDDEF--DDEFDDEHDEFSH------EHGHGLPNNMMPMMGSKGGGSFG---ANG 240

Query: 241 MINFNAMN-GKKGGDGGTA-------------------KKDSG----------------- 300
           MI+ N+MN GKKGG GG A                   KKD G                 
Sbjct: 241 MIDINSMNGGKKGGVGGGAVKKGEAIDFPVMMKGKGGGKKDGGHGDGDGGGGGGGGGGGG 300

Query: 301 ---------------------------SKKNKGSSNHKSAENGGGKHNGNGAKKGGKNDG 360
                                      SKKNKGSS  K+A+  GGKHNGNG KKGGK D 
Sbjct: 301 GTKSKSKGGGFLGLGKKGLFGFGGKNDSKKNKGSS--KAAD--GGKHNGNGGKKGGKADA 360

Query: 361 LHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHA-NGNKGQMGHMGQM 420
           LHEFDKLDI FHD+DITKPSKGGK   G  GGNGKNM Q   MGHA NGNK QMG     
Sbjct: 361 LHEFDKLDINFHDIDITKPSKGGK---GGSGGNGKNMAQT-HMGHATNGNKSQMGQ---- 420

Query: 421 GPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGL---PAQPMMN-GGGGYYQGMGQGNPY 472
                MG  G NR PPMGNYAMNQMGNIPAV+GL   PAQPMM+ GGGGYYQGMGQGNPY
Sbjct: 421 ----PMGPAGANRAPPMGNYAMNQMGNIPAVRGLPAHPAQPMMSGGGGGYYQGMGQGNPY 480

BLAST of Bhi02G001681 vs. ExPASy TrEMBL
Match: A0A5D3CIN1 (Cell wall protein IFF6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003730 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.2e-197
Identity = 416/584 (71.23%), Postives = 435/584 (74.49%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQAC LRVNIHCDGCKQKVKKILQKI+GVYTVKID+EQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACVLRVNIHCDGCKQKVKKILQKIEGVYTVKIDSEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSN-----NNMNNFNNQFKQMQFQNVK-----GGVGGKDNNM 120
           IKKLEKSGKHAELWG QISSN     NN NNFNNQFKQMQFQNVK     GG GGKDNNM
Sbjct: 61  IKKLEKSGKHAELWGKQISSNNMNNFNNYNNFNNQFKQMQFQNVKGGGGGGGGGGKDNNM 120

Query: 121 NNHKFQMEKAFLQHHQQQQQQQLKGS----NKGGG-NNNNNMHSLMVPPKDKKSVKFNLP 180
           NNHKFQMEKA+LQ HQQQQQQQLKGS    NKGG   NNNNMHSLMVPPKDKKSVKFNLP
Sbjct: 121 NNHKFQMEKAYLQ-HQQQQQQQLKGSNNITNKGGNITNNNNMHSLMVPPKDKKSVKFNLP 180

Query: 181 EEDSDFSDDEF-DDEFDDEFDDD--DEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKK 240
           EE+SDFS DEF DDEFDD+FDDD  D+ DDEDDEFSHGHGH  GHGHGLPNNMMPVMGKK
Sbjct: 181 EEESDFSGDEFDDDEFDDDFDDDDYDDEDDEDDEFSHGHGH--GHGHGLPNNMMPVMGKK 240

Query: 241 GGSFGPNESNGMINFNAMNGKKGGDGGTA------------------------------- 300
           GGSFG NE NGM++FNAMNGKKGGDGG A                               
Sbjct: 241 GGSFGANEHNGMMSFNAMNGKKGGDGGNAKKGEVIDFPIMMKSKGGGNEGKAGKGGGKKD 300

Query: 301 ----------------------------------------------------KKDSGSKK 360
                                                               KKD+G KK
Sbjct: 301 GNSSGSDSGTKSKSKGGDQKKVGKDKNGGKKGGGFLGLGKKGLFGFGGKNGSKKDNGGKK 360

Query: 361 NKGSS-NHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSG-- 420
           NKGSS N+KS+ENGGGKHN NGAKKGGKNDGLHEFDK+D+GFHDLDI KPSKGGK +G  
Sbjct: 361 NKGSSNNNKSSENGGGKHNSNGAKKGGKNDGLHEFDKMDLGFHDLDIAKPSKGGKVNGGG 420

Query: 421 -----GNGGGNGKN---MGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNY 472
                GNG GN KN   M QMGQ+GH NGN+GQ         MG MGH G +RGPPMGNY
Sbjct: 421 NGNGNGNGNGNSKNMSQMNQMGQLGHGNGNRGQ---------MGPMGHAGVSRGPPMGNY 480

BLAST of Bhi02G001681 vs. ExPASy TrEMBL
Match: A0A1S3BP40 (cell wall protein IFF6-like OS=Cucumis melo OX=3656 GN=LOC103492186 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.2e-197
Identity = 416/584 (71.23%), Postives = 435/584 (74.49%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQAC LRVNIHCDGCKQKVKKILQKI+GVYTVKID+EQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACVLRVNIHCDGCKQKVKKILQKIEGVYTVKIDSEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSN-----NNMNNFNNQFKQMQFQNVK-----GGVGGKDNNM 120
           IKKLEKSGKHAELWG QISSN     NN NNFNNQFKQMQFQNVK     GG GGKDNNM
Sbjct: 61  IKKLEKSGKHAELWGKQISSNNMNNFNNYNNFNNQFKQMQFQNVKGGGGGGGGGGKDNNM 120

Query: 121 NNHKFQMEKAFLQHHQQQQQQQLKGS----NKGGG-NNNNNMHSLMVPPKDKKSVKFNLP 180
           NNHKFQMEKA+LQ HQQQQQQQLKGS    NKGG   NNNNMHSLMVPPKDKKSVKFNLP
Sbjct: 121 NNHKFQMEKAYLQ-HQQQQQQQLKGSNNITNKGGNITNNNNMHSLMVPPKDKKSVKFNLP 180

Query: 181 EEDSDFSDDEF-DDEFDDEFDDD--DEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKK 240
           EE+SDFS DEF DDEFDD+FDDD  D+ DDEDDEFSHGHGH  GHGHGLPNNMMPVMGKK
Sbjct: 181 EEESDFSGDEFDDDEFDDDFDDDDYDDEDDEDDEFSHGHGH--GHGHGLPNNMMPVMGKK 240

Query: 241 GGSFGPNESNGMINFNAMNGKKGGDGGTA------------------------------- 300
           GGSFG NE NGM++FNAMNGKKGGDGG A                               
Sbjct: 241 GGSFGANEHNGMMSFNAMNGKKGGDGGNAKKGEVIDFPIMMKSKGGGNEGKAGKGGGKKD 300

Query: 301 ----------------------------------------------------KKDSGSKK 360
                                                               KKD+G KK
Sbjct: 301 GNSSGSDSGTKSKSKGGDQKKVGKDKNGGKKGGGFLGLGKKGLFGFGGKNGSKKDNGGKK 360

Query: 361 NKGSS-NHKSAENGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSG-- 420
           NKGSS N+KS+ENGGGKHN NGAKKGGKNDGLHEFDK+D+GFHDLDI KPSKGGK +G  
Sbjct: 361 NKGSSNNNKSSENGGGKHNSNGAKKGGKNDGLHEFDKMDLGFHDLDIAKPSKGGKVNGGG 420

Query: 421 -----GNGGGNGKN---MGQMGQMGHANGNKGQMGHMGQMGPMGQMGHGGGNRGPPMGNY 472
                GNG GN KN   M QMGQ+GH NGN+GQ         MG MGH G +RGPPMGNY
Sbjct: 421 NGNGNGNGNGNSKNMSQMNQMGQLGHGNGNRGQ---------MGPMGHAGVSRGPPMGNY 480

BLAST of Bhi02G001681 vs. ExPASy TrEMBL
Match: A0A6J1E4L0 (heavy metal-associated isoprenylated plant protein 34 OS=Momordica charantia OX=3673 GN=LOC111026005 PE=4 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.3e-183
Identity = 392/571 (68.65%), Postives = 414/571 (72.50%), Query Frame = 0

Query: 2   SKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKLI 61
           +KQDFM IQACALRVNIHC+GCKQKVKKILQKIDGVYTVKIDAEQGKV+VTGNIDP KLI
Sbjct: 3   NKQDFMKIQACALRVNIHCEGCKQKVKKILQKIDGVYTVKIDAEQGKVSVTGNIDPAKLI 62

Query: 62  KKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKAF 121
           +KLEKSGKHAELWGAQ++  +N NN NNQFK MQFQNVKGG GGKDNNM N+KFQMEKAF
Sbjct: 63  QKLEKSGKHAELWGAQLNP-SNFNN-NNQFKSMQFQNVKGGGGGKDNNMMNNKFQMEKAF 122

Query: 122 LQHHQQQQQQQLKG--SNKGGGNNNNNMHSLMVPPKDKKSVKFNLPEEDSDFSDDEFDDE 181
           LQ  QQQQQQQLKG  +NKGG +N NN+++LM PPKDKKSVKFNLPEE SDFS DEFDDE
Sbjct: 123 LQQQQQQQQQQLKGFSNNKGGNHNLNNLNNLMAPPKDKKSVKFNLPEEVSDFSGDEFDDE 182

Query: 182 FDDEFDD-DDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKKGGSF---GPNESNGMI 241
           FDDEFDD DDEFDDEDDE   GHGH  GHGH LPN MMP+MGK GGS    GP   NGMI
Sbjct: 183 FDDEFDDEDDEFDDEDDEQEFGHGH--GHGHALPNKMMPMMGKGGGSLGSNGPMGPNGMI 242

Query: 242 NFNAMNGKKGGDGGT--------------------------------------------- 301
           NFN MN KK GDG                                               
Sbjct: 243 NFNMMNSKKSGDGAAKKGEVIDFPVMMKGNKGGGGGNEGKNSKGGGKKDGGGGGGGGGGG 302

Query: 302 ---------------------------------------AKKDSGSKKNKGSSNHKSAE- 361
                                                   KK+SG  K KGSSN+KS+E 
Sbjct: 303 GGNKSKSKGGGEQKKEGKKGGGFLGLGKKGLFGFGGKNGGKKESGHSKKKGSSNNKSSEG 362

Query: 362 NGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMG 421
            GGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDI+KPSKGGKG+    GGNGK+MGQMG
Sbjct: 363 GGGGKHNGNGAKKGGKNDGLHEFDKLDIGFHDLDISKPSKGGKGN----GGNGKSMGQMG 422

Query: 422 QMGHANGNKGQMGHMGQMGPM-------GQMGHGGGNRGPPMGNYAMNQMGNIPAVQGLP 472
           QMGH N NK QMG MGQMG M       GQMG GG N GPPMGNY M QMGNIPAVQGLP
Sbjct: 423 QMGHGNANKSQMGQMGQMGQMGPGGINRGQMGPGGANMGPPMGNYPMTQMGNIPAVQGLP 482

BLAST of Bhi02G001681 vs. ExPASy TrEMBL
Match: A0A0A0LWG7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570180 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 4.3e-166
Identity = 395/717 (55.09%), Postives = 418/717 (58.30%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQAC LRVNIHCDGCKQKV+KILQKI+GVYTVKID+EQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACVLRVNIHCDGCKQKVRKILQKIEGVYTVKIDSEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSN-----NNMNNFNNQFKQMQFQNVK--GGVGGKDNNMNNH 120
           IKKLEKSGKHAELWG QISSN     NN NNFNNQFKQMQFQNVK  GG GGKDNNMNNH
Sbjct: 61  IKKLEKSGKHAELWGKQISSNNMNNFNNYNNFNNQFKQMQFQNVKGGGGGGGKDNNMNNH 120

Query: 121 KFQMEKAFLQH-HQQQQQQQLKGS----NKGGG-NNNNNMHSLMVPPKDKKSVKFNLPEE 180
           K QMEKA+LQH  QQQQQQQLKGS    NKGG  +NNNNMHSLMVPPKDKKSVKFNLPEE
Sbjct: 121 KLQMEKAYLQHQQQQQQQQQLKGSNNITNKGGNISNNNNMHSLMVPPKDKKSVKFNLPEE 180

Query: 181 DSDFSDDEF-DDEFDDEFDDD--------------------------------------- 240
           +SDFS DEF DDEFDDEFDDD                                       
Sbjct: 181 ESDFSGDEFDDDEFDDEFDDDDYDDEDDEDDEDDEDDEDDEDDEDDEDDEDDEDDEDDED 240

Query: 241 --------------------DEFDDEDDEFS----------------------------- 300
                               D+ DDEDD+FS                             
Sbjct: 241 DEDDEDDEDDEDDEDDEDDEDDEDDEDDDFSHGHGHGHGHGHGHGHGHGHGHGHGHGHGH 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGHGH 360

Query: 361 -------HGHGHGNGHGHGLPNNMMPVMGKKGGSFGPNESNGMINFNAMNGKKGGDGGTA 420
                  HGHGHG+GHGHGLPNNM+P MGKKGGSFG NE     NFN MNGKKGGDGG A
Sbjct: 361 GHGHGHGHGHGHGHGHGHGLPNNMLPGMGKKGGSFGANEPVAYTNFNPMNGKKGGDGGYA 420

Query: 421 ------------------------------------------------------------ 462
                                                                       
Sbjct: 421 KKGEVIDFPIVMKSKGGGNEGKGGKGGGKKDGNSSGSDSGTKSKSKGGDQKKGGKKGGGF 480

BLAST of Bhi02G001681 vs. ExPASy TrEMBL
Match: A0A6J1HAD8 (heavy metal-associated isoprenylated plant protein 34-like OS=Cucurbita moschata OX=3662 GN=LOC111462066 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 5.2e-164
Identity = 381/549 (69.40%), Postives = 399/549 (72.68%), Query Frame = 0

Query: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60
           MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL
Sbjct: 1   MSKQDFMNIQACALRVNIHCDGCKQKVKKILQKIDGVYTVKIDAEQGKVTVTGNIDPGKL 60

Query: 61  IKKLEKSGKHAELWGAQISSNNNMNNFNNQFKQMQFQNVKGGVGGKDNNMNNHKFQMEKA 120
           IKKLEKSGKHAELWGAQISS NNMNNFNN      FQNVK   GGKDNNM+NHK  MEKA
Sbjct: 61  IKKLEKSGKHAELWGAQISS-NNMNNFNN-----LFQNVK---GGKDNNMDNHKLLMEKA 120

Query: 121 FLQHHQQQQQQQLKGS----NKGGGNN--NNNMHSLMVPPKDKKSVKFNLPEEDSDFSDD 180
           FL     QQQQQ+KGS    NKGGGNN  NNNMHS++VP KDKKSVKFNLPEEDS+FS D
Sbjct: 121 FL-----QQQQQMKGSNNGMNKGGGNNNHNNNMHSVVVPGKDKKSVKFNLPEEDSEFS-D 180

Query: 181 EFDDEFDDEFDDDDEFDDEDDEFSHGHGHGNGHGHGLPNNMMPVMGKK-GGSFGPNESNG 240
           EFDDEFDDEF  DDEFDDE DEFSH       HGHGLPNNMMP+MG K GGSFG   +NG
Sbjct: 181 EFDDEFDDEF--DDEFDDEHDEFSH------EHGHGLPNNMMPMMGSKGGGSFG---ANG 240

Query: 241 MINFNAMN-GKKGGDGGTA-------------------KKDSG----------------- 300
           MI+ N+MN GKKGG GG A                   KKD G                 
Sbjct: 241 MIDINSMNGGKKGGVGGGAVKKGEAIDFPVMMKGKGGGKKDGGHGDGDGGGGGGGGGGGG 300

Query: 301 ---------------------------SKKNKGSSNHKSAENGGGKHNGNGAKKGGKNDG 360
                                      SKKNKGSS  K+A+  GGKHNGNG KKGGK D 
Sbjct: 301 GTKSKSKGGGFLGLGKKGLFGFGGKNDSKKNKGSS--KAAD--GGKHNGNGGKKGGKADA 360

Query: 361 LHEFDKLDIGFHDLDITKPSKGGKGSGGNGGGNGKNMGQMGQMGHA-NGNKGQMGHMGQM 420
           LHEFDKLDI FHD+DITKPSKGGK   G  GGNGKNM Q   MGHA NGNK QMG     
Sbjct: 361 LHEFDKLDINFHDIDITKPSKGGK---GGSGGNGKNMAQT-HMGHATNGNKSQMGQ---- 420

Query: 421 GPMGQMGHGGGNRGPPMGNYAMNQMGNIPAVQGL---PAQPMMN-GGGGYYQGMGQGNPY 472
                MG  G NR PPMGNYAMNQMGNIPAV+GL   PAQPMM+ GGGGYYQGMGQGNPY
Sbjct: 421 ----PMGPAGANRAPPMGNYAMNQMGNIPAVRGLPAHPAQPMMSGGGGGYYQGMGQGNPY 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G05220.14.1e-6841.19Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.13.8e-6641.37Heavy metal transport/detoxification superfamily protein [more]
AT3G06130.17.2e-6542.91Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.22.1e-5639.29Heavy metal transport/detoxification superfamily protein [more]
AT5G19090.32.1e-5639.29Heavy metal transport/detoxification superfamily protein [more]
Match NameE-valueIdentityDescription
Q0WV375.7e-6741.19Heavy metal-associated isoprenylated plant protein 34 OS=Arabidopsis thaliana OX... [more]
F4JZL75.4e-6541.37Heavy metal-associated isoprenylated plant protein 33 OS=Arabidopsis thaliana OX... [more]
Q9M8K51.0e-6342.91Heavy metal-associated isoprenylated plant protein 32 OS=Arabidopsis thaliana OX... [more]
Q84J885.3e-2053.92Heavy metal-associated isoprenylated plant protein 36 OS=Arabidopsis thaliana OX... [more]
A2RVM82.2e-1853.33Heavy metal-associated isoprenylated plant protein 37 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_038879083.13.1e-25184.71heavy metal-associated isoprenylated plant protein 34-like [Benincasa hispida][more]
XP_008450667.12.6e-19771.23PREDICTED: cell wall protein IFF6-like [Cucumis melo] >TYK10226.1 cell wall prot... [more]
XP_031741111.16.1e-19170.49heavy metal-associated isoprenylated plant protein 34 [Cucumis sativus] >KAE8653... [more]
XP_022159661.14.7e-18368.65heavy metal-associated isoprenylated plant protein 34 [Momordica charantia][more]
XP_022961512.11.1e-16369.40heavy metal-associated isoprenylated plant protein 34-like [Cucurbita moschata] ... [more]
Match NameE-valueIdentityDescription
A0A5D3CIN11.2e-19771.23Cell wall protein IFF6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BP401.2e-19771.23cell wall protein IFF6-like OS=Cucumis melo OX=3656 GN=LOC103492186 PE=4 SV=1[more]
A0A6J1E4L02.3e-18368.65heavy metal-associated isoprenylated plant protein 34 OS=Momordica charantia OX=... [more]
A0A0A0LWG74.3e-16655.09HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570180 PE=4 SV... [more]
A0A6J1HAD85.2e-16469.40heavy metal-associated isoprenylated plant protein 34-like OS=Cucurbita moschata... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 14..70
e-value: 2.7E-17
score: 62.9
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 17..73
score: 12.8819
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 14..72
e-value: 3.41847E-14
score: 65.3197
NoneNo IPR availableGENE3D3.30.70.100coord: 8..78
e-value: 6.2E-21
score: 76.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 281..308
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..324
NoneNo IPR availablePANTHERPTHR45868:SF40GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKEcoord: 1..206
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 252..471
NoneNo IPR availablePANTHERPTHR45868HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATEDcoord: 1..206
NoneNo IPR availablePANTHERPTHR45868:SF40GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1-LIKEcoord: 252..471
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 11..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001681Bhi02M001681mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding