Bhi02G001606 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001606
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionVacuolar protein sorting-associated protein 32-like protein 2
Locationchr2: 52072981 .. 52076992 (-)
RNA-Seq ExpressionBhi02G001606
SyntenyBhi02G001606
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAAACTGTGTCTTGTAACTTTCCCTATCACACAATGCTCACAAAGTGTAAGCTGATCACAAATACCTTTAGGTAGAATGCCTTGTCTATATTTTTATTGATATTTTCATAAAATTAAGAGTCGACATTTTAATCCCGGTACATTTTGCCTATATGGACCTTTGATGGATATTCTTTTTTATTGTCGTGAGTTGTGACCGATGTGGAACCCAACGGCGCTGTCAACGCCGTGAAGAAAAAGGCTAAGGATTATGAAGCTTCCTCTCTCTCTCTCGCATCCTACACTAAAACAACACGCCTAAGCAAAGCCGAATCCAATTCGCCTATCAACCAGATCTCCCCTTAATTCTTTACTTTCAATTTCTTCACACCGATCGCTCTTCGATTTCCGATCATCAATTCTCAGGTACATACTTCTTCATCAATCTTTTGTTTTTCTTGTTTCAGTTTGGTTGTTGACCATCTTCTTTTTTTTTTTATCCAATTCCCCGTCTGTTTCAATCCCGATTCCGGTCGAGTTTCGAGAATTCATCTCTTCTCAGACCAGATATGATTGGTTATTTTCTTGCTATTGCTCCAGAAGTTTGGAGGTCGGTTCGAATCGCACATTTTCCGCCATGTCAATGTTCAATAAGCTTTTTGGTAAACCTAAACAGGAGTCTAATGCCCTGGCCACGCTTGATAAATTAAACGAGGTATCATCTATCATGCTGNNNNNNNNNNNNNNNCACCCCAATTTTGCTTATTTGGGATTCTCGTGTGTTTTTTAAACCACCAATTTGGCTAATTATTTCGAATTAGTTGACACGAACTAGAGGGATTTGTCCTAGGCTGAAAGGAATTCTTTTATGCTCTGTAATTTTTCAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCTGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGGGGTATGACTTTAAGTTTAAATAAATGCTGGCAGTTTGTTATGAGCGAAACATTTTGGCATTAAGTACTACAAGTATTGAAATATAATGATGTGAGTAAATTTGCTAGCTTGTTGATAATTAAGTCAAGGGTGACATAGCAATGCAGTTTAAGTTGTAACACTATAGTAAATTTAAATACTTTTTCTTCTTCTCGTGTTTGGGGGTTCTTTGGTTGTTATTTATATATTTTTTTCTTTAGTGTAAGCTCTACCTTCTTTTCCTCCTTATGATTTTTTTCATTTTAAATATTTTGAAAGTTAGAGAGATGGTTCGGAATCTCCGGACACTCCTACGTGTAAAAAGTACTGATTTCATAATTTCTAAAATTGTGCCTGTGAAGTTTGGGTGCTTCCTAGTCAAGGAGATAACTTGTTGATTTGGTTACAGACATATTTTAACCATCTATGTAATTGTAAACGGTAATTGCTTGTTTTGGCCTTCTTTTATGCCTCTTTGTCAAATTTCATCTTTTTTGTTAGTTGTGTTTGTTACTTATTCAAATTAGGGATGTGTTTCATTTTCAATGCGGATGCAAGACGTTCATTTGTTTGTACTTCTTGATTTTGGGGTATCTGCATTTCATTTTTCACATTTCTTATCACAAATTTGTAGAATCCTGTTACCTTCTTATGTTTATTGTGTTAATATCTTGGGGCAGTTTATAATAGGATCCATTTGTTTTGGTTAAAAAAAAAGGGATCTATTTGTTCCACCTTTATATTGGAACTTTCCACTTTCAATTTGCAATTCAAACTGTAGTTGGAGATGTGGCGTTGTTGTTAACTATTGTAATTTTATAGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATTCATGATCAGGTTATCTTTGATGTTATATCTGATATGAGACTAGTTAGACGTATGCATGTTTCCTTTTGCTGCATGTTTATGCAATTTTGTGTTACATATTTGTGTTGCAGATGATATTACTCGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGTGAGTACCGCATTGTTTCAGTGTACTTTGATGATTAGTTAAATTTAGATGTTTTGAGTCCGTCCATATTTTTTTTGTTCTCTTTCCCAACCTGAAGATTGTGAGAATCTTTTGAATTATGTGCTGGCAGAGCATAACGACACATGAAACCTATGTTCCCATTAAATTCTATGAAACTGCATGTTTAGTGCTTAACAAGGAGTTTAGAAGATGAGGTTGATGATGGAACTTGAAATATTCTTCTTTTGCAGGAATATTGATGATGTAGACAAAACAATGGATGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTGTCAAATCCAATTGGTGCTGCAGCCGATTTTGATGAGGTTTTTATTCACTTTCAGTTTATCTTTTCATTTGTACATGCAATTTTATGAAGTTTATCTACTAAACACATCTTTGTACGCACAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCTGAGTTGGAAGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCTCCAATTTCAGTCCCTGCAGGCCGACAAACTGCTCGACCCGTGCCTCAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGATGTGATATCCAACAATGTTCTGAAGCAGGTCCTCGGGTTAATCCTGTGATGAGTTCTTTCCCTTTGTGAGCAATGAATATCCTACATACATGCCCTAGGCCATCTAAAGACTAAATTGTTGTTTATTGATCGTGCCTGAAAACATCAACATTTGTGCATTGTACCTGAATTTGCATCTAGAGCTAAAGCTGCCAAGGCTAAAAGATTTTTATGGAATTTTGCAGGTGGCGAGAATTTGAAAGAGAGGCGATGTGAAGAATTGGCCCGAGGTTGTTCTGAAGATTTGAACATTTTTTGCTATGTTCAGCAAAGCCTGATGAACAAGGTCTCTTTGTGATAATACCAGACAATAATGCAAATTTAAATTAGGTCTTGTATACTCTGTAAATGAATATAACATTAATGTGGTTAGTATTTGACCTAATATGGAGCTGATGTGTGTGTCTCAATTACACGAATGCTGGGTGTTATATTTGAGTTGGTTATTTGAAAATCCATGTGTTCGGTTGATTATTTAAAAAATAAGGGCATCTGATGTTCTCTAAATTAAATTATAAATTTAATCTCTAAACTTTGAGGTTAAGCATCTTTGTACCAAAAATTTCAAAATTACAAATAAGGTCATCCAGTGCTCCATGTATTTCAGGTTATAATGTGTATTTCCCCAAGCCCTAAGCTTAGTTTAAGATTCCATTAGAAATAAAATTAAAATTTATATCTAATCTAATACATCAAATTCTGTAAACTCTTCTGTGTAGCTCGAAACATAGGAATTCATTGTAATACATCAGACTGTTCCAATGCCTATAACGACCAAGAGAAGAGAACAAATTACAGATTCATGAGCCTGTGTCGGTTTCACATGAAACATCGCTTAGTGTTCTGGGATCGGAGAAAAATTCATTTAAGAATCGACACATTTCTTATTTACCACGTAACTAATGAACGGGCGAACTATACACATGATGGGTGACTTTCAGGTGACAAACTGTAGCCCATGTTCTATAATGTTTGCTTCAGAATATCATTTGAGACATCAAGCATACGGTTAAATCAGCTTTGAAGCTGCTGCTCACTTAATCAATAACTGAACTCAGAGGGGCTGGTGACAACTCCAAATAATCAAAATCGTCCGTCATGCCAACCTACATTACATTAACAAAGATTAAAATATCGATATCCATATCAATGACGATATTGAGATTTTAATTTTATGAATATATCGATATCGATAGATATTTCTGCAAGTCACAGAATTTATAAAATTTATTTAGATTAATAAA

mRNA sequence

GTAAAACTGTGTCTTGTAACTTTCCCTATCACACAATGCTCACAAAGTGTAAGCTGATCACAAATACCTTTAGTTGTGACCGATGTGGAACCCAACGGCGCTGTCAACGCCGTGAAGAAAAAGGCTAAGGATTATGAAGCTTCCTCTCTCTCTCTCGCATCCTACACTAAAACAACACGCCTAAGCAAAGCCGAATCCAATTCGCCTATCAACCAGATCTCCCCTTAATTCTTTACTTTCAATTTCTTCACACCGATCGCTCTTCGATTTCCGATCATCAATTCTCAGAAGTTTGGAGGTCGGTTCGAATCGCACATTTTCCGCCATGTCAATGTTCAATAAGCTTTTTGGTAAACCTAAACAGGAGTCTAATGCCCTGGCCACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCTGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGGGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATTCATGATCAGATGATATTACTCGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATTGATGATGTAGACAAAACAATGGATGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTGTCAAATCCAATTGGTGCTGCAGCCGATTTTGATGAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCTGAGTTGGAAGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCTCCAATTTCAGTCCCTGCAGGCCGACAAACTGCTCGACCCGTGCCTCAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGATGTGATATCCAACAATGTTCTGAAGCAGGTGGCGAGAATTTGAAAGAGAGGCGATGTGAAGAATTGGCCCGAGGTTGTTCTGAAGATTTGAACATTTTTTGCTATGTTCAGCAAAGCCTGATGAACAAGGTCTCTTTGTGATAATACCAGACAATAATGCAAATTTAAATTAGGTCTTGTATACTCTGTAAATGAATATAACATTAATGTGGTTAGTATTTGACCTAATATGGAGCTGATGTGTGTGTCTCAATTACACGAATGCTGGGTGTTATATTTGAGTTGGTTATTTGAAAATCCATGTGTTCGGTTGATTATTTAAAAAATAAGGGCATCTGATGTTCTCTAAATTAAATTATAAATTTAATCTCTAAACTTTGAGGTTAAGCATCTTTGTACCAAAAATTTCAAAATTACAAATAAGGTCATCCAGTGCTCCATGTATTTCAGGTTATAATGTGTATTTCCCCAAGCCCTAAGCTTAGTTTAAGATTCCATTAGAAATAAAATTAAAATTTATATCTAATCTAATACATCAAATTCTGTAAACTCTTCTGTGTAGCTCGAAACATAGGAATTCATTGTAATACATCAGACTGTTCCAATGCCTATAACGACCAAGAGAAGAGAACAAATTACAGATTCATGAGCCTGTGTCGGTTTCACATGAAACATCGCTTAGTGTTCTGGGATCGGAGAAAAATTCATTTAAGAATCGACACATTTCTTATTTACCACGTAACTAATGAACGGGCGAACTATACACATGATGGGTGACTTTCAGGTGACAAACTGTAGCCCATGTTCTATAATGTTTGCTTCAGAATATCATTTGAGACATCAAGCATACGGTTAAATCAGCTTTGAAGCTGCTGCTCACTTAATCAATAACTGAACTCAGAGGGGCTGGTGACAACTCCAAATAATCAAAATCGTCCGTCATGCCAACCTACATTACATTAACAAAGATTAAAATATCGATATCCATATCAATGACGATATTGAGATTTTAATTTTATGAATATATCGATATCGATAGATATTTCTGCAAGTCACAGAATTTATAAAATTTATTTAGATTAATAAA

Coding sequence (CDS)

ATGTCAATGTTCAATAAGCTTTTTGGTAAACCTAAACAGGAGTCTAATGCCCTGGCCACGCTTGATAAATTAAACGAGACACTGGAAATGCTGGAGAAGAAGGAGAAAGTACTTTTGAAAAAGGCTGCCACAGAGGTTGAGAAGGCCAAAGATTATACCAGAGCAAAGAACAAGCGGGCTGCTATACAATGTTTGAAGAGGAAGAGGCTATATGAACAACAGATAGAGCAGCTCGGCAACTTCCAATTGCGGATTCATGATCAGATGATATTACTCGAAGGTGCAAAAGCCACAACTGAGACTGTAGATGCATTGAGGACAGGGGCAGCTGCAATGAAAGCAATGCAGAAAGCAACGAATATTGATGATGTAGACAAAACAATGGATGAGATCAATGAACAAACTGAGAACATGAAACAGATCCAAGAAGCATTGTCAAATCCAATTGGTGCTGCAGCCGATTTTGATGAGGATGAACTGGAAGCAGAACTTGAAGAGCTAGAAGGTGCTGAGTTGGAAGAGCAACTTCTTCAACCTGCAACAACTGCTCCTGCTGCTCCAATTTCAGTCCCTGCAGGCCGACAAACTGCTCGACCCGTGCCTCAGAAACGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAGGCTGAGATGGCACTTTGA

Protein sequence

MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL
Homology
BLAST of Bhi02G001606 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 345.5 bits (885), Expect = 3.1e-95
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE+NAL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-ISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           AP  P + VPAGRQ ARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Bhi02G001606 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 345.5 bits (885), Expect = 3.1e-95
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE+NAL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-ISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           AP  P + VPAGRQ ARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Bhi02G001606 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-85
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 5   NKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQC 64
           N+LFGKPKQE++ L TLDKLNETLEMLEKKE VLLKKA  EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 65  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 124
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 125 DKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATTAP 184
           DKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 185 AAPISVPAGRQTARPVPQKR-TAEEDELAALQAEMAL 221
             PI VP G + AR   QK+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Bhi02G001606 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 300.8 bits (769), Expect = 8.8e-82
Identity = 168/192 (87.50%), Postives = 179/192 (93.23%), Query Frame = 0

Query: 30  MLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 89
           MLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 90  ILLEGAKATTETVDALRTGAAAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSNPI 149
           I+LEGAKATTETVDALR+GA+AMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 150 GAAADFDEDELEAELEELEGAELEEQLLQPATTAPAAP-ISVPAGRQTARPVPQKRTAEE 209
           GAAADFDEDEL AEL+ELE  ELE QLLQPATTAP  P + VPAGRQ ARPVPQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 210 DELAALQAEMAL 221
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of Bhi02G001606 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 55.1 bits (131), Expect = 8.4e-08
Identity = 52/180 (28.89%), Postives = 84/180 (46.67%), Query Frame = 0

Query: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 62
           M N    KPK      A L  L      L + ++ L K    E + A+D  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G  A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182
           DV K MD+  E      ++   L   + A    DE+E+ AE + LE   + E + +  TT
Sbjct: 121 DVQKLMDDTAEAKAYQDELSAILGEKLSAE---DEEEILAEFDNLESLLIVEDMPEVPTT 176

BLAST of Bhi02G001606 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 4.4e-94
Identity = 191/219 (87.21%), Postives = 204/219 (93.15%), Query Frame = 0

Query: 3   MFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAI 62
           M N+LFGKPKQE+NAL TLDKLNETLEMLEKKEKVLLKKA  EVEKAK+Y+RAKNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 63  QCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNID 122
           QCLKRKRLYE Q+EQLGNFQLRIHDQMI+LEGAKATTETVDALR+GA+AMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 123 DVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATT 182
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELE  ELE QLLQPATT
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 183 APAAP-ISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           AP  P + VPAGRQ ARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Bhi02G001606 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 5.4e-84
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 5   NKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRAAIQC 64
           N+LFGKPKQE++ L TLDKLNETLEMLEKKE VLLKKA  EVEKAK+++RAKNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 65  LKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATNIDDV 124
           LKRKRLYEQQ+EQLGNFQLRIHDQMI+LEGAKATTETVDALRTGA+AMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 125 DKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPATTAP 184
           DKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELEGAELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 185 AAPISVPAGRQTARPVPQKR-TAEEDELAALQAEMAL 221
             PI VP G + AR   QK+ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Bhi02G001606 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.8e-26
Identity = 89/219 (40.64%), Postives = 126/219 (57.53%), Query Frame = 0

Query: 1   MSMFNKLF---GKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKN 60
           MS+F K+F   GK  + ++    + +L ET EML KK++ L KK   E+  AK     KN
Sbjct: 1   MSLFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLL 180
             +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQA 217
           +     P    +VP+    +RP  +K   +ED++  L+A
Sbjct: 181 EIGDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of Bhi02G001606 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.7e-26
Identity = 87/218 (39.91%), Postives = 125/218 (57.34%), Query Frame = 0

Query: 1   MSMFNKLF---GKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKN 60
           MS+  KLF   GK  +  +    + KL +T EML KK++ L KK   E+  AK +   KN
Sbjct: 1   MSLIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLL 180
             +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQ 216
           +          +VPA    A+PV +K+  ++D++  L+
Sbjct: 181 EVQGPETVPLPNVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of Bhi02G001606 vs. ExPASy Swiss-Prot
Match: Q6GL11 (Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 87/218 (39.91%), Postives = 125/218 (57.34%), Query Frame = 0

Query: 1   MSMFNKLF---GKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKN 60
           MS+  KLF   GK  +  +    + KL +T EML KK++ L KK   E+  AK +   KN
Sbjct: 1   MSLIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLTKKQEFLEKKIEQELVTAKKH-GTKN 60

Query: 61  KRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQK 120
           KRAA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA   
Sbjct: 61  KRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHD 120

Query: 121 ATNIDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLL 180
             +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL
Sbjct: 121 NMDIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLL 180

Query: 181 QPATTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQ 216
           +          +VPA    A+PV +K+  ++D++  L+
Sbjct: 181 EVQGPETVPLPNVPAAVLPAKPVKKKQEEDDDDMRELE 217

BLAST of Bhi02G001606 vs. NCBI nr
Match: XP_038880238.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida])

HSP 1 Score: 397.1 bits (1019), Expect = 9.7e-107
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. NCBI nr
Match: XP_008450809.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008450810.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_016900986.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >TYK10128.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPI VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. NCBI nr
Match: XP_004135686.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011659962.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031737801.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN66200.1 hypothetical protein Csa_006989 [Cucumis sativus])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELE AELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPI VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. NCBI nr
Match: XP_022961241.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961242.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961243.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022961244.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022987856.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987857.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987858.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >XP_022987859.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita maxima] >KAG7023632.1 Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 216/220 (98.18%), Postives = 216/220 (98.18%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAP API VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. NCBI nr
Match: XP_023516619.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023516620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.1 bits (993), Expect = 1.0e-103
Identity = 215/220 (97.73%), Postives = 215/220 (97.73%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAP A I VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPVASIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. ExPASy TrEMBL
Match: A0A6J1JBI2 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485275 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.7e-105
Identity = 216/220 (98.18%), Postives = 216/220 (98.18%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAP API VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. ExPASy TrEMBL
Match: A0A1S3BQS1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103492284 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.7e-105
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPI VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. ExPASy TrEMBL
Match: A0A6J1H9T9 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111461810 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.7e-105
Identity = 216/220 (98.18%), Postives = 216/220 (98.18%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKE VLLKKAATEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKENVLLKKAATEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAP API VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPVAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. ExPASy TrEMBL
Match: A0A5D3CFX2 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002710 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.7e-105
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSM NKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMLNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPI VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

BLAST of Bhi02G001606 vs. ExPASy TrEMBL
Match: A0A0A0LWC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G575160 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.7e-105
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0

Query: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKAATEVEKAKDYTRAKNKRA 60
           MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKA+TEVEKAKDYTRAKNKRA
Sbjct: 1   MSMFNKLFGKPKQESNALATLDKLNETLEMLEKKEKVLLKKASTEVEKAKDYTRAKNKRA 60

Query: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120
           AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN
Sbjct: 61  AIQCLKRKRLYEQQIEQLGNFQLRIHDQMILLEGAKATTETVDALRTGAAAMKAMQKATN 120

Query: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELEGAELEEQLLQPA 180
           IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELE AELEEQLLQPA
Sbjct: 121 IDDVDKTMDEINEQTENMKQIQEALSNPIGAAADFDEDELEAELEELESAELEEQLLQPA 180

Query: 181 TTAPAAPISVPAGRQTARPVPQKRTAEEDELAALQAEMAL 221
           TTAPAAPI VPAGRQTARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TTAPAAPIPVPAGRQTARPAPQKRTAEEDELAALQAEMAL 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G29160.13.1e-9587.21SNF7 family protein [more]
AT4G29160.33.1e-9587.21SNF7 family protein [more]
AT2G19830.13.8e-8583.41SNF7 family protein [more]
AT4G29160.28.8e-8287.50SNF7 family protein [more]
AT5G09260.18.4e-0828.89vacuolar protein sorting-associated protein 20.2 [more]
Match NameE-valueIdentityDescription
Q9SZE44.4e-9487.21Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821975.4e-8483.41Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC41.8e-2640.64Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q5XGW66.7e-2639.91Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Q6GL111.1e-2539.91Charged multivesicular body protein 4b OS=Xenopus tropicalis OX=8364 GN=chmp4b P... [more]
Match NameE-valueIdentityDescription
XP_038880238.19.7e-107100.00vacuolar protein sorting-associated protein 32 homolog 2 [Benincasa hispida][more]
XP_008450809.11.2e-10498.18PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
XP_004135686.11.2e-10498.18vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
XP_022961241.11.2e-10498.18vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
XP_023516619.11.0e-10397.73vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
A0A6J1JBI25.7e-10598.18vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita maxim... [more]
A0A1S3BQS15.7e-10598.18vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A6J1H9T95.7e-10598.18vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A5D3CFX25.7e-10598.18Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
A0A0A0LWC65.7e-10598.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G575160 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..177
NoneNo IPR availableCOILSCoilCoilcoord: 124..144
NoneNo IPR availableCOILSCoilCoilcoord: 14..41
NoneNo IPR availableGENE3D1.10.287.1060coord: 16..93
e-value: 3.8E-21
score: 77.0
NoneNo IPR availableGENE3D6.10.250.1710coord: 119..174
e-value: 5.9E-9
score: 37.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 177..207
NoneNo IPR availablePANTHERPTHR22761:SF53VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 2-LIKEcoord: 3..220
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 3..220
IPR005024Snf7 familyPFAMPF03357Snf7coord: 22..187
e-value: 1.1E-46
score: 158.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001606Bhi02M001606mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body