Bhi02G001275 (gene) Wax gourd (B227) v1

BLAST of Bhi02G001275 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.4e-152
Identity = 260/492 (52.85%), Postives = 354/492 (71.95%), Query Frame = 0

Query: 54  LPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQ 113
           LPPGPK WPL+G LP IL    P ++WIHS+MK+ NT+IA IRL NT++IPVTSP +A +
Sbjct: 34  LPPGPKSWPLIGNLPEILGRNKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSPRIARE 93

Query: 114 FLKTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLG 173
            LK  DS+FA+R     T +  SRGYLT A    G QWKKMRR++AS V +  S   ML 
Sbjct: 94  ILKKQDSVFATRPLTMGT-EYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQ 153

Query: 174 QRTAEADNLLCYIFILTSKNGGGA--VINVRSITQHYCGNIIRRMLFNRRYYGKGREDG- 233
           +RT EADNL+ YI   + KN G A  VI++R   + Y GN+ R+M+F  R++GKG EDG 
Sbjct: 154 KRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGS 213

Query: 234 GPTSEEEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIID 293
           GP  EE +H E+L T+L H+ AF++SD+VP L+  DL+GHEK++  A+  V KY++P +D
Sbjct: 214 GPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVD 273

Query: 294 ERVQQWKDKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWA 353
           ER+ QW++ K++  +D LD+ I  KD  G   LS EEIKAQ+TEL +AT+DNPSNA EW 
Sbjct: 274 ERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEWG 333

Query: 354 MAELINQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHV 413
           MAE+IN+P I++KAVEE+D VVG++RLV ESD+P L Y+ AC++E+FRLHP +PFN+PH+
Sbjct: 334 MAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHM 393

Query: 414 SNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLR 473
           S +D +V+GY IPKGS VL+SR+G+GRNP +W+ P +F+PERHL  +T  +L  +E  L 
Sbjct: 394 STTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTCVDL--NESDLN 453

Query: 474 FITFTRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQL 533
            I+F+ GRRGC+G  +G+ +T ML ARL+QGF+W   PG  K+D SE+ + L +  PL  
Sbjct: 454 IISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKPLYA 513

Query: 534 HAQPRLSHSMYP 543
            A PRL+  +YP
Sbjct: 514 VATPRLAPHVYP 522

BLAST of Bhi02G001275 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 500.0 bits (1286), Expect = 2.5e-141
Identity = 241/492 (48.98%), Postives = 349/492 (70.93%), Query Frame = 0

Query: 54  LPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQ 113
           LPPGP  WP++G +PT+L ++ P ++W+HS+MKQ NTEIA ++LGNT++I VT P++A +
Sbjct: 56  LPPGPTGWPIIGMIPTMLKSR-PVFRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIARE 115

Query: 114 FLKTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLG 173
            LK  D++FASR  ++    ILS GY T   +  G+Q+KKMR+++ +E++ P     +  
Sbjct: 116 ILKQQDALFASR-PLTYAQKILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQ 175

Query: 174 QRTAEADNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKG-REDGGP 233
           +R+ E D+L  +++ +   +G    ++ R +T+HYCGN I++++F  R + K    DGGP
Sbjct: 176 KRSEENDHLTAWVYNMVKNSGS---VDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGP 235

Query: 234 TSEEEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDER 293
           T E+ +H EA+   L    AF ISD++P L   DL+GHEKIM+++  ++ KY +PIIDER
Sbjct: 236 TVEDVEHMEAMFEALGFTFAFCISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDER 295

Query: 294 VQQWKDKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMA 353
           ++ W++ K   IED LDI IS+KD+ GN LL+ +EIK  I EL +A  DNPSNAVEWAMA
Sbjct: 296 IKMWREGKRTQIEDFLDIFISIKDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMA 355

Query: 354 ELINQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSN 413
           E++N+PEIL+KA+EE+D VVG+ERLV+ESDIPKL Y+ A +RE+FRLHP + FN+PHV+ 
Sbjct: 356 EMVNKPEILRKAMEEIDRVVGKERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVAL 415

Query: 414 SDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFI 473
           SD  V GY IPKGS+VLLSR GLGRNP++W DP+ F PERHL +   +E+ ++E  LRFI
Sbjct: 416 SDTTVAGYHIPKGSQVLLSRYGLGRNPKVWADPLCFKPERHLNE--CSEVTLTENDLRFI 475

Query: 474 TFTRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHA 533
           +F+ G+RGC   +LGT +T M+ ARLLQGF+W LP   T+++  E+ H + +  PL +  
Sbjct: 476 SFSTGKRGCAAPALGTALTTMMLARLLQGFTWKLPENETRVELMESSHDMFLAKPLVMVG 535

Query: 534 QPRLSHSMYPNL 545
             RL   +YP +
Sbjct: 536 DLRLPEHLYPTV 540

BLAST of Bhi02G001275 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 494.2 bits (1271), Expect = 1.4e-139
Identity = 247/527 (46.87%), Postives = 357/527 (67.74%), Query Frame = 0

Query: 19  AITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQA--PLPPGPKPWPLVGCLPTILTNKSP 78
           + T  +++    A  ++ F    N+ +  S  +   PLPPGP  +P+VG +P +L N+ P
Sbjct: 21  SFTNMYLLTTLQALAALCFLMILNKIKSSSRNKKLHPLPPGPTGFPIVGMIPAMLKNR-P 80

Query: 79  TYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFASRSSISNTVDILS 138
            ++W+HS+MK+ NTEIA +RLGNT++IPVT P++A +  K  D++FASR  ++    ILS
Sbjct: 81  VFRWLHSLMKELNTEIACVRLGNTHVIPVTCPKIAREIFKQQDALFASR-PLTYAQKILS 140

Query: 139 RGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLLCYIFILTSKNGGG 198
            GY T   +  G Q+KKMR+++ +E++ P     +   R  E D+L  +++ +   +   
Sbjct: 141 NGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKNS--- 200

Query: 199 AVINVRSITQHYCGNIIRRMLFNRRYYGKGRE-DGGPTSEEEDHNEALLTILRHVNAFSI 258
             +++R +T+HYCGN I+R++F  R + +  E DGGPT E+ +H +A+   L    AF I
Sbjct: 201 EPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFCI 260

Query: 259 SDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKKIRVIEDILDILISLK 318
           SD++P L   DL+GHEKIM+++  ++ KY +PIIDER++ W++ K   IED LDI IS+K
Sbjct: 261 SDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISIK 320

Query: 319 DDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEILKKAVEELDEVVGRE 378
           D+ G  LL+ +EIK  I EL +A  DNPSNAVEWA+AE+IN+PEIL KA+EE+D VVG+E
Sbjct: 321 DEAGQPLLTADEIKPTIKELVMAAPDNPSNAVEWAIAEMINKPEILHKAMEEIDRVVGKE 380

Query: 379 RLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYLIPKGSEVLLSRIGL 438
           R V+ESDIPKL Y+ A IRE+FRLHP + FN+PHV+ SD  V GY IPKGS+VLLSR GL
Sbjct: 381 RFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYGL 440

Query: 439 GRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRGCVGSSLGTNITMMLF 498
           GRNP++W DP+ F PERHL +   +E+ ++E  LRFI+F+ G+RGC   +LGT IT M+ 
Sbjct: 441 GRNPKVWSDPLSFKPERHLNE--CSEVTLTENDLRFISFSTGKRGCAAPALGTAITTMML 500

Query: 499 ARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSMYP 543
           ARLLQGF W L    T+++  E+ H + +  PL L  + RLS  +YP
Sbjct: 501 ARLLQGFKWKLAGSETRVELMESSHDMFLSKPLVLVGELRLSEDLYP 540

BLAST of Bhi02G001275 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 463.0 bits (1190), Expect = 3.4e-130
Identity = 239/494 (48.38%), Postives = 324/494 (65.59%), Query Frame = 0

Query: 54  LPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQ 113
           LPPGPK WPLVG LP IL    P ++WIHS+M++ NT+IA IRL NT++IPVTSP +A +
Sbjct: 42  LPPGPKSWPLVGNLPEILGRNKPVFRWIHSLMEELNTDIACIRLANTHVIPVTSPRIARE 101

Query: 114 FLKTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLG 173
            LK  DS+FA R     T +  SRGYLT A  S G QWKKMRR++AS V +  S   ML 
Sbjct: 102 ILKKQDSVFAIRPLTMGT-EYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFKLMLE 161

Query: 174 QRTAEADNLLCYIFILTSKNGGG----AVINVRSITQHYCGNIIRRMLFNRRYYGKGRED 233
           +RT EADNL+ YI     KN G     AVI++R + + Y GN+ R+M+F  R++GKG ED
Sbjct: 162 KRTEEADNLVRYINNRCVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSED 221

Query: 234 -GGPTSEEEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPI 293
             GP SEE +H E+L T+L H+ AF++SD+VP L+  DL+GHEK++  A+  V KY++P 
Sbjct: 222 ESGPGSEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPF 281

Query: 294 IDERVQQWKDKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVE 353
           +DER+ QW++ K++  +D LD+ I  KD +G   LS EEIKAQ+TEL +AT+DNPSNA E
Sbjct: 282 VDERLMQWRNGKMKEPQDFLDMFIMAKDTNGKPTLSEEEIKAQVTELMLATVDNPSNAAE 341

Query: 354 WAMAELINQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIP 413
           WAMAE+IN+P I++KAVEE+D    RE + E               E+FRLHP +PFN+P
Sbjct: 342 WAMAEMINEPSIMQKAVEEIDRSNLRESVCE---------------ETFRLHPVAPFNLP 401

Query: 414 HVSNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPS 473
           H+S +D +V+GY IPKGS +L+SR+G+GRNP +W+ P++FNPERHL ++           
Sbjct: 402 HMSTADAVVDGYFIPKGSHMLISRMGIGRNPNVWDKPLKFNPERHLGNN----------- 461

Query: 474 LRFITFTRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPL 533
                              +++T ML ARL+QGF+WS  PG +K+D SE+   L +  PL
Sbjct: 462 -------------------SSMTYMLLARLIQGFTWSPVPGESKIDISESKSDLFMAKPL 489

Query: 534 QLHAQPRLSHSMYP 543
              A PRL   +YP
Sbjct: 522 HAVATPRLDPHVYP 489

BLAST of Bhi02G001275 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-118
Identity = 209/521 (40.12%), Postives = 335/521 (64.30%), Query Frame = 0

Query: 27  FVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMK 86
           F  +  +  L      R  +    +  LPPGP+ WP+VG +  ++ N+ P + WIH VM+
Sbjct: 10  FTLVLISITLVLALARRFSRFMKPKGQLPPGPRGWPIVGNMLQMIINR-PAHLWIHRVME 69

Query: 87  QFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFASRSSISNTVDILSRGYLTTAFSS 146
           +  T+IA  R    ++I VTS ++A + L+  D + A RS  S    ++S GY   +FSS
Sbjct: 70  ELQTDIACYRFARFHVITVTSSKIAREVLREKDEVLADRSE-SYASHLISHGYKNISFSS 129

Query: 147 MGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLLCYIFILTSKNGGGAVINVRSITQ 206
            G  WK +++++ +++++PT+L + LG R  EADN++ Y++ L         INVR    
Sbjct: 130 YGENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLGSVRKPINVRDTIL 189

Query: 207 HYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHNEALLTILRHVNAFSISDFVPCLKPFD 266
            YC  ++ RM+F +R++ +  E+GG   +E++H +A+   L    +F++++++P L+ ++
Sbjct: 190 TYCHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSFNLTNYIPFLRGWN 249

Query: 267 LDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKKIRVI-EDILDILISLKDDHGNSLLSI 326
           +D  E  +++A++++   ++PII ER+  W+ K  + + ED LDILI+LKDD G  L + 
Sbjct: 250 VDKAETEVREAVHIINICNDPIIQERIHLWRKKGGKQMEEDWLDILITLKDDQGMHLFTF 309

Query: 327 EEIKAQITELQIATIDNPSNAVEWAMAELINQPEILKKAVEELDEVVGRERLVEESDIPK 386
           +EI+AQ  E+ +ATIDN  N VEW +AE++N PEIL+KA  ELD +VG++RLV+ESDI +
Sbjct: 310 DEIRAQCKEINLATIDNTMNNVEWTIAEMLNHPEILEKATNELDIIVGKDRLVQESDISQ 369

Query: 387 LKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDP 446
           L Y+ AC +ESFRLHP + F   HV+  D  + GY +PKGS++L+SR+GLGRNP+IW++P
Sbjct: 370 LNYIKACSKESFRLHPANVFMPHHVAREDTTLAGYFVPKGSQILVSRLGLGRNPKIWDEP 429

Query: 447 MRFNPERHLKDDTMTELGIS--EPSLRFITFTRGRRGCVGSSLGTNITMMLFARLLQGFS 506
             F PER+L       LG++  EP +RF+TF  GRR C G+ +GT++T+ML ARL+QGF 
Sbjct: 430 NAFKPERYLDGHVEKSLGVTLMEPDMRFVTFGTGRRSCPGTKIGTSMTIMLLARLIQGFE 489

Query: 507 WSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSMYPNL 545
           W+LP G + ++    +  L +  PL   A+PRL+ S+YP +
Sbjct: 490 WTLPIGKSSVELISAESNLFMAKPLLACAKPRLAPSLYPKI 528

BLAST of Bhi02G001275 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.4e-168
Identity = 289/542 (53.32%), Postives = 393/542 (72.51%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKP 60
           M   +LF+   +    PI+++  FI+F+F+    +L +H      K SV   PLPPGP P
Sbjct: 1   MSLPYLFLD--SEVTPPISLSLAFIIFMFLV-KFILKTH-----NKNSVPVVPLPPGPSP 60

Query: 61  WPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDS 120
           WP+VG LP +  N+ P ++WIHS+MK+ NT+IA IRLGN ++IPVTSPE+A +FLK  D+
Sbjct: 61  WPIVGSLPEMWRNR-PAHRWIHSLMKKLNTDIACIRLGNVHVIPVTSPEIAREFLKKNDA 120

Query: 121 IFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEAD 180
           +FASR  ++     LS GYLTT     G+QW+KMRR+L +E  NP+ +H +LG+R  EAD
Sbjct: 121 VFASR-PVTMATKTLSSGYLTTVVGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEAD 180

Query: 181 NLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHN 240
           NL+ +++   S N  GAV+NVR   Q Y G+I+R+M+FNR Y+GKGREDGGP  EEE+H 
Sbjct: 181 NLVKFLYNQCSANQNGAVVNVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHV 240

Query: 241 EALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKK 300
            ALLT+L +  AF +SD++P L+ FD+DGHEK ++KA+N+V+K+ EPI++ER+Q+W+D K
Sbjct: 241 SALLTLLTYAYAFCVSDYLPWLRVFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGK 300

Query: 301 IRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEI 360
               +D+LD+ ISLKD +G  LLS EEIKAQ TELQ++T+D+P N  EWA+ E++NQPE+
Sbjct: 301 RNEPDDLLDVFISLKDANGQPLLSDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEM 360

Query: 361 LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGY 420
           LKKA EELD VVG++ LV+ES +P L Y+ AC +E  RLHP  PFN+PHVS +D  V GY
Sbjct: 361 LKKAEEELDRVVGKKTLVQESHVPHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGY 420

Query: 421 LIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRG 480
            IPKGS V+LSR+ LGRNPR+WE+P+RFNPERHL      ++ + E  LRF++F+ GRRG
Sbjct: 421 FIPKGSNVILSRLELGRNPRVWEEPLRFNPERHLNIAVDQQVDLEENDLRFVSFSTGRRG 480

Query: 481 CVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSM 540
           C+G  LG+ I +ML ARLLQGFSWSLPP + K+DF+E    L   +PL   A+PRL  S+
Sbjct: 481 CMGVGLGSTIVVMLLARLLQGFSWSLPPDVDKIDFTEDQIYLKKASPLLAQAKPRLPASV 532

Query: 541 YP 543
           YP
Sbjct: 541 YP 532

BLAST of Bhi02G001275 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 276/519 (53.18%), Postives = 369/519 (71.10%), Query Frame = 0

Query: 25  IVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKPWPLVGCLPTILTNKSPTYQWIHSV 84
           ++F FM F  V  +H  N+ +K       LPPGPKPWP+VG LP +L N+ P   WIH +
Sbjct: 22  VIFSFMIF-KVTKTHLVNKSKKYK-----LPPGPKPWPIVGNLPEMLANR-PATIWIHKL 81

Query: 85  MKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFASRSSISNTVDILSRGYLTTAF 144
           MK+ NTEIA IRL NT +IPVT P +A +FLK +D+ FASR  I +T DI S G++TT  
Sbjct: 82  MKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMST-DIASDGFITTVL 141

Query: 145 SSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLLCYIFILTSK--NGGGAVINVR 204
              G QWKKM+R+L + +L+P     +LG+R  EADNL+ YI+    K  N G  ++N+R
Sbjct: 142 VPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLVNIR 201

Query: 205 SITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHNEALLTILRHVNAFSISDFVPCL 264
              QHY GN+ R+++FN RY+GK  EDGGP  EE +H  A  TIL++V AFSISDFVP L
Sbjct: 202 IAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFVPFL 261

Query: 265 KPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKKIRVIEDILDILISLKDDHGNSL 324
           +  DLDGH   + KA+ ++RKY +PIID+R++QW D    V ED+LD+LI LKD +   L
Sbjct: 262 RRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDLLDVLIKLKDANNKPL 321

Query: 325 LSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEILKKAVEELDEVVGRERLVEESD 384
           L+++E+KAQI EL I  +DNPSNA EWA+AE+INQPE+LK+A EELD VVG+ERLV+ESD
Sbjct: 322 LTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNVVGKERLVQESD 381

Query: 385 IPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIW 444
           IPKL+++ AC RE+ RLHP   FN+PH+  +D +V  YL PKG++VLLSR+ LGRNP+ W
Sbjct: 382 IPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNPKFW 441

Query: 445 EDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRGCVGSSLGTNITMMLFARLLQGF 504
            DP++FNPERHLK+    ++ ++EP LRFI+FT GRR C G +LGT +T+MLFAR+L GF
Sbjct: 442 TDPLKFNPERHLKEG--IDVVLTEPDLRFISFTTGRRSCPGVALGTTMTVMLFARMLHGF 501

Query: 505 SWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSMY 542
           SWS PP ++ +D   +   L +  PL L A+PRL+  +Y
Sbjct: 502 SWSPPPDVSSIDLVPSKDDLFLAKPLLLVAKPRLAAELY 530

BLAST of Bhi02G001275 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-157
Identity = 274/519 (52.79%), Postives = 370/519 (71.29%), Query Frame = 0

Query: 25  IVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKPWPLVGCLPTILTNKSPTYQWIHSV 84
           ++F F+ F  V  SH  N+ +K       LPPGPKPWP+VG LP +L N+ P   WIH +
Sbjct: 22  VIFFFIIF-KVTKSHSVNKSKKYK-----LPPGPKPWPIVGNLPEMLANR-PATIWIHKL 81

Query: 85  MKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFASRSSISNTVDILSRGYLTTAF 144
           MK+ NTEIA IRL NT +IPVT P +A +FLK +D+ FASR  I +T DI S G+LTT  
Sbjct: 82  MKEMNTEIACIRLANTIVIPVTCPTIACEFLKKHDASFASRPKIMST-DIASDGFLTTVL 141

Query: 145 SSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLLCYIFILTSK--NGGGAVINVR 204
              G QWKKM+R+L + +L+P     +LG+R  EADNL+ YI+    K  N G  ++N+R
Sbjct: 142 VPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKDVNDGPGLVNIR 201

Query: 205 SITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHNEALLTILRHVNAFSISDFVPCL 264
              QHY GN+ R+++FN RY+GK  EDGGP  EE +H  A  TIL++V AFSISDF+P L
Sbjct: 202 IAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVYAFSISDFIPFL 261

Query: 265 KPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKKIRVIEDILDILISLKDDHGNSL 324
           +  DLDGH   + KA+ +++KY +PII +R++QW D    V ED+LD+LI LKD     L
Sbjct: 262 RRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDLLDVLIKLKDASNKPL 321

Query: 325 LSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEILKKAVEELDEVVGRERLVEESD 384
           L+++EIKAQITEL I  +DNPSNA EWA+AE++NQPE+LK+A EELD VVG+ERLV+ESD
Sbjct: 322 LTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNVVGKERLVQESD 381

Query: 385 IPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIW 444
           IPKL+++ AC RE+ RLHP   FN+PH+  +D +V  YL PKG++VLLSR+ LGRNP+ W
Sbjct: 382 IPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLSRVALGRNPKFW 441

Query: 445 EDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRGCVGSSLGTNITMMLFARLLQGF 504
            DP++FNPERHLK+    ++ ++EP LRFI+FT GRR C G +LGT +T+MLFAR+L GF
Sbjct: 442 TDPLKFNPERHLKEG--IDVVLTEPDLRFISFTTGRRSCPGVTLGTTMTIMLFARMLHGF 501

Query: 505 SWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSMY 542
           SWS PP ++ +D +++   L +  PL + A+PRL+  +Y
Sbjct: 502 SWSAPPNVSSIDLTQSSDDLFMAKPLCVVAKPRLAAELY 530

BLAST of Bhi02G001275 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.0e-151
Identity = 260/492 (52.85%), Postives = 354/492 (71.95%), Query Frame = 0

Query: 54  LPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQ 113
           LPPGPK WPL+G LP IL    P ++WIHS+MK+ NT+IA IRL NT++IPVTSP +A +
Sbjct: 40  LPPGPKSWPLIGNLPEILGRNKPVFRWIHSLMKELNTDIACIRLANTHVIPVTSPRIARE 99

Query: 114 FLKTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLG 173
            LK  DS+FA+R     T +  SRGYLT A    G QWKKMRR++AS V +  S   ML 
Sbjct: 100 ILKKQDSVFATRPLTMGT-EYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQ 159

Query: 174 QRTAEADNLLCYIFILTSKNGGGA--VINVRSITQHYCGNIIRRMLFNRRYYGKGREDG- 233
           +RT EADNL+ YI   + KN G A  VI++R   + Y GN+ R+M+F  R++GKG EDG 
Sbjct: 160 KRTEEADNLVRYINNRSVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGS 219

Query: 234 GPTSEEEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIID 293
           GP  EE +H E+L T+L H+ AF++SD+VP L+  DL+GHEK++  A+  V KY++P +D
Sbjct: 220 GPGLEEIEHVESLFTVLTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVD 279

Query: 294 ERVQQWKDKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWA 353
           ER+ QW++ K++  +D LD+ I  KD  G   LS EEIKAQ+TEL +AT+DNPSNA EW 
Sbjct: 280 ERLMQWRNGKMKEPQDFLDMFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEWG 339

Query: 354 MAELINQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHV 413
           MAE+IN+P I++KAVEE+D VVG++RLV ESD+P L Y+ AC++E+FRLHP +PFN+PH+
Sbjct: 340 MAEMINEPSIMQKAVEEIDRVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHM 399

Query: 414 SNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLR 473
           S +D +V+GY IPKGS VL+SR+G+GRNP +W+ P +F+PERHL  +T  +L  +E  L 
Sbjct: 400 STTDTVVDGYFIPKGSHVLISRMGIGRNPSVWDKPHKFDPERHLSTNTCVDL--NESDLN 459

Query: 474 FITFTRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQL 533
            I+F+ GRRGC+G  +G+ +T ML ARL+QGF+W   PG  K+D SE+ + L +  PL  
Sbjct: 460 IISFSAGRRGCMGVDIGSAMTYMLLARLIQGFTWLPVPGKNKIDISESKNDLFMAKPLYA 519

Query: 534 HAQPRLSHSMYP 543
            A PRL+  +YP
Sbjct: 520 VATPRLAPHVYP 528

BLAST of Bhi02G001275 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 533.9 bits (1374), Expect = 2.2e-150
Identity = 253/497 (50.91%), Postives = 360/497 (72.43%), Query Frame = 0

Query: 49  VQQAPLPPGPKPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSP 108
           V   PLPPGP PWP+VG LP +L NK P ++WIH +M++  T+IA ++LG  +++ +T P
Sbjct: 63  VGNPPLPPGPVPWPVVGNLPEMLLNK-PAFRWIHQMMREMGTDIACVKLGGVHVVSITCP 122

Query: 109 ELALQFLKTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSL 168
           E+A + L+  D+ F SR  ++   +  S GY     S  G+QWKKMRR+L SE++ P+  
Sbjct: 123 EIAREVLRKQDANFISR-PLTFASETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRH 182

Query: 169 HQMLGQRTAEADNLLCYIFILTSKNGGGAV-INVRSITQHYCGNIIRRMLFNRRYYGKGR 228
             +  +RT EADNL  Y++ L +K   G V ++VR + +HYCGN+IRR++FNRRY+G+ +
Sbjct: 183 AWLHDKRTDEADNLTRYVYNLATKAATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQ 242

Query: 229 EDGGPTSEEEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEP 288
            DGGP   E  H +A+ T L  + AF +SD++P L+  DLDGHEKI+K+A   V +  + 
Sbjct: 243 ADGGPGPMEVLHMDAVFTSLGLLYAFCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDT 302

Query: 289 IIDERVQQWKDKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAV 348
           +ID+R +QWK  + + +ED LD+LI+LKD  GN LL+IEE+KAQ  ++  A +DNPSNAV
Sbjct: 303 VIDDRWRQWKSGERQEMEDFLDVLITLKDAQGNPLLTIEEVKAQSQDITFAAVDNPSNAV 362

Query: 349 EWAMAELINQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNI 408
           EWA+AE++N PE++ KA+EELD VVGRERLV+ESDIPKL Y+ ACIRE+FRLHP +PFN+
Sbjct: 363 EWALAEMVNNPEVMAKAMEELDRVVGRERLVQESDIPKLNYVKACIREAFRLHPVAPFNV 422

Query: 409 PHVSNSDIIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEP 468
           PHV+ +D  + GY +PKGS V+LSR GLGRNPR+W++P+RF P+RHL     +++ ++E 
Sbjct: 423 PHVALADTTIAGYRVPKGSHVILSRTGLGRNPRVWDEPLRFYPDRHLA-TAASDVALTEN 482

Query: 469 SLRFITFTRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTP 528
            LRFI+F+ GRRGC+ +SLGT +++MLF RLLQGF+WS P G+  +D SE+     + TP
Sbjct: 483 DLRFISFSTGRRGCIAASLGTAMSVMLFGRLLQGFTWSKPAGVEAVDLSESKSDTFMATP 542

Query: 529 LQLHAQPRLSHSMYPNL 545
           L LHA+PRL   +YP++
Sbjct: 543 LVLHAEPRLPAHLYPSI 556

BLAST of Bhi02G001275 vs. NCBI nr
Match: XP_038880015.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKP 60
           MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKP
Sbjct: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKP 60

Query: 61  WPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDS 120
           WPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDS
Sbjct: 61  WPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDS 120

Query: 121 IFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEAD 180
           IFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEAD
Sbjct: 121 IFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEAD 180

Query: 181 NLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHN 240
           NLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHN
Sbjct: 181 NLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHN 240

Query: 241 EALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKK 300
           EALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKK
Sbjct: 241 EALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKK 300

Query: 301 IRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEI 360
           IRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEI
Sbjct: 301 IRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEI 360

Query: 361 LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGY 420
           LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGY
Sbjct: 361 LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGY 420

Query: 421 LIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRG 480
           LIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRG
Sbjct: 421 LIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRG 480

Query: 481 CVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSM 540
           CVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSM
Sbjct: 481 CVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSM 540

Query: 541 YPNLIDHEPRPT 553
           YPNLIDHEPRPT
Sbjct: 541 YPNLIDHEPRPT 552

BLAST of Bhi02G001275 vs. NCBI nr
Match: XP_038878794.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.1e-289
Identity = 503/553 (90.96%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSV-QQAPLPPGPK 60
           MEAAFL ISQLA+Q+S IAI+KTFIVFVFMAF SV+FSH YN + K SV ++APLPPGPK
Sbjct: 1   MEAAFLIISQLAYQNSLIAISKTFIVFVFMAFISVIFSHCYNLKTKRSVRRKAPLPPGPK 60

Query: 61  PWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYD 120
           PWPLVGCLPT+LT K P +QWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLK YD
Sbjct: 61  PWPLVGCLPTMLTIKYPPHQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKIYD 120

Query: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEA 180
           SIFASRSSISNTVDILSRGYLTTAFSSMG+QW+KMRRILASE+LNP  LH+MLGQRT+EA
Sbjct: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGDQWRKMRRILASEILNPKMLHRMLGQRTSEA 180

Query: 181 DNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 240
           D+LL YIF LTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPT EEEDH
Sbjct: 181 DSLLRYIFTLTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTFEEEDH 240

Query: 241 NEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDK 300
           N+ALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMK+ALN+VRKYDEPIIDERVQ WK K
Sbjct: 241 NQALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKEALNLVRKYDEPIIDERVQHWKVK 300

Query: 301 KIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPE 360
           KIRVIEDILDILISLKDD+GNSLLSIEEIKAQI ELQIATIDNPSNAVEWAMAELINQPE
Sbjct: 301 KIRVIEDILDILISLKDDNGNSLLSIEEIKAQIVELQIATIDNPSNAVEWAMAELINQPE 360

Query: 361 ILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNG 420
           +LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDI+VNG
Sbjct: 361 VLKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIVVNG 420

Query: 421 YLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRR 480
           YLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDT+TELGISEPSLRFITFTRGRR
Sbjct: 421 YLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTVTELGISEPSLRFITFTRGRR 480

Query: 481 GCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHS 540
           GCVGSSLGTNITMMLFARLLQGF WSL PGITKMDFSE DH+LS+PTPLQL+ QPRLSHS
Sbjct: 481 GCVGSSLGTNITMMLFARLLQGFFWSLLPGITKMDFSEIDHELSLPTPLQLYVQPRLSHS 540

Query: 541 MYPNLIDHEPRPT 553
           MYPNL DHEPRPT
Sbjct: 541 MYPNLFDHEPRPT 553

BLAST of Bhi02G001275 vs. NCBI nr
Match: XP_008450367.2 (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis melo])

HSP 1 Score: 930.2 bits (2403), Expect = 8.0e-267
Identity = 460/551 (83.48%), Postives = 513/551 (93.10%), Query Frame = 0

Query: 1   MEAAF-LFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPK 60
           MEAAF +FI+QL HQ+SPIAI+K FIVF+FMAF SV+FS  YNRR+K SV++A LPPGPK
Sbjct: 1   MEAAFSIFITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSVRKALLPPGPK 60

Query: 61  PWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYD 120
           PWP+VGCLPT+LTNKSPTYQWIHSVMKQFNTEIA IRLGNTY+IPVTSPELAL+FL+T+D
Sbjct: 61  PWPIVGCLPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTHD 120

Query: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEA 180
           S+F+SRSSISNTVDILSRGYLTTAFSSMG+QWKKMRRILASE+L+PT LH+ML QR AEA
Sbjct: 121 SVFSSRSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAEA 180

Query: 181 DNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 240
           D LL YIF +TS+NGGGAVINVRSITQHYCGNIIR MLFNRRYYGKGREDGGPTSEEE+H
Sbjct: 181 DTLLHYIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEEH 240

Query: 241 NEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQW-KD 300
           N+ALLTILRH+NAFSISDF+PCLKPFDLDGHEKIMK ALN VRKYDEPIIDERVQQW KD
Sbjct: 241 NQALLTILRHINAFSISDFMPCLKPFDLDGHEKIMKNALNAVRKYDEPIIDERVQQWKKD 300

Query: 301 KKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQP 360
           KKI V +DILDILIS KDD+GNSLLSI+EIKAQITELQIATIDNPSNAVEWAMAELINQP
Sbjct: 301 KKIEV-KDILDILISRKDDNGNSLLSIDEIKAQITELQIATIDNPSNAVEWAMAELINQP 360

Query: 361 EILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVN 420
           E+LKK V+ELD VVG+ERLV+E DIPKLKYLTAC+RESFRLHPFSPFN+PHV   DI+VN
Sbjct: 361 EVLKKVVDELDNVVGKERLVQEYDIPKLKYLTACLRESFRLHPFSPFNVPHVPTFDIVVN 420

Query: 421 GYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGR 480
           GYLIPKGSEVLLSR+GLGRNPRIW+DP++FNPERHLKD+T+ ELG+SEP+LRFITF+RGR
Sbjct: 421 GYLIPKGSEVLLSRLGLGRNPRIWKDPIKFNPERHLKDNTV-ELGLSEPNLRFITFSRGR 480

Query: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSH 540
           RGCVGSSLGTNIT+MLFARLLQGFSWSL PGIT++DFS+ D +  +  PLQLHA+PRLS 
Sbjct: 481 RGCVGSSLGTNITVMLFARLLQGFSWSLLPGITQIDFSKVDREGLLLNPLQLHAEPRLSR 540

Query: 541 SMYPNLIDHEP 550
           SMYP LIDHEP
Sbjct: 541 SMYPMLIDHEP 549

BLAST of Bhi02G001275 vs. NCBI nr
Match: XP_004151661.2 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8653524.1 hypothetical protein Csa_007554 [Cucumis sativus])

HSP 1 Score: 920.2 bits (2377), Expect = 8.3e-264
Identity = 455/545 (83.49%), Postives = 502/545 (92.11%), Query Frame = 0

Query: 1   MEAAFL-FISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAP-LPPGP 60
           MEA FL F++QL HQHSPIAI+KTFI+F+FMAF SV+FSH+YNRR+K SV + P LPPGP
Sbjct: 1   MEAGFLIFLTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGP 60

Query: 61  KPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTY 120
           KPWP+VGCLPT+LTNKSPT  WIHSVMKQFNTEIA IRLG+TYIIPVTSPELA++FLKT+
Sbjct: 61  KPWPIVGCLPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKTH 120

Query: 121 DSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAE 180
           DS+F+SRSSISNTV ILSRGYLTTAFSSMG+QWKKMRRILASE+LNPT LHQMLGQRTAE
Sbjct: 121 DSVFSSRSSISNTVHILSRGYLTTAFSSMGDQWKKMRRILASEILNPTMLHQMLGQRTAE 180

Query: 181 ADNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEED 240
           AD LL YIF +TS NGG AVINVRSITQHYCGNIIR MLFNRRYYGKGREDGGPT EEE+
Sbjct: 181 ADTLLRYIFSITSGNGGSAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTLEEEE 240

Query: 241 HNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKD 300
           HN+ALLTILRHVNAFSISDF+PCLKPFDLDGHEKIMK ALNVVRK+DEPIIDERV+QWK 
Sbjct: 241 HNQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKNALNVVRKHDEPIIDERVKQWKK 300

Query: 301 KKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQP 360
            K    +DILDILISLKDD+GNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAE+INQP
Sbjct: 301 DKKLEGKDILDILISLKDDNGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAEIINQP 360

Query: 361 EILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVN 420
           E+LKK V+ELD+VVG+ERLV+ESDIPKLKYLTAC+RESFRLHPFSPFNIPHVS  DI+VN
Sbjct: 361 EVLKKVVKELDDVVGKERLVQESDIPKLKYLTACLRESFRLHPFSPFNIPHVSKFDIVVN 420

Query: 421 GYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGR 480
           GYLIPKGSEVLLSR GLGRNPRIWEDP++FNPERHLK D   ELG+SEPSLRFITF+RGR
Sbjct: 421 GYLIPKGSEVLLSRFGLGRNPRIWEDPIKFNPERHLK-DASEELGLSEPSLRFITFSRGR 480

Query: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSH 540
           RGCVGSSLGTNITMMLFARLLQGFSWSL PGI ++DFS+ DH+  +  PL+LHA+PRLSH
Sbjct: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLLPGIKEIDFSKMDHEHLLLNPLKLHAEPRLSH 540

Query: 541 SMYPN 544
            MYP+
Sbjct: 541 GMYPS 544

BLAST of Bhi02G001275 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 874.4 bits (2258), Expect = 5.2e-250
Identity = 433/546 (79.30%), Postives = 493/546 (90.29%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQHSPIAIT-KTFIVFVFMAFTSVLFSHFY-NRRRKLSVQQAPLPPGP 60
           M  AFLFI QLAHQ+SPIAIT KT IVF FMA TS+LFS  Y NRRRK  V++ PLPPGP
Sbjct: 1   MVIAFLFIPQLAHQNSPIAITSKTLIVFGFMALTSILFSQCYCNRRRKHLVRRPPLPPGP 60

Query: 61  KPWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTY 120
           KPWPLVGCLPT+L NKSPT+QWIHSVMK+ NTEIASIRLGNTY+IPVTSPELAL+FLKTY
Sbjct: 61  KPWPLVGCLPTMLINKSPTHQWIHSVMKKLNTEIASIRLGNTYVIPVTSPELALEFLKTY 120

Query: 121 DSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAE 180
           DS+FASRSS SN + IL+RGY++T  S +G+QWKKMRRIL S++LNP++LH+MLGQRTAE
Sbjct: 121 DSVFASRSSTSNAIYILTRGYVSTILSPLGDQWKKMRRILTSKILNPSTLHRMLGQRTAE 180

Query: 181 ADNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEED 240
           AD LL YIF  T+ N GGAVIN+RSITQHYCGNI+R+MLFNRRYYGKGREDGGPT EEE+
Sbjct: 181 ADTLLRYIFTQTNNNRGGAVINLRSITQHYCGNILRQMLFNRRYYGKGREDGGPTFEEEE 240

Query: 241 HNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKD 300
           H +ALLTI++H++AFSISDF+PCLK FDLDGH+KIMK ALN+++KYDEPIIDERVQ+WKD
Sbjct: 241 HVQALLTIVKHIHAFSISDFMPCLKSFDLDGHQKIMKNALNILQKYDEPIIDERVQEWKD 300

Query: 301 KKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQP 360
           KKI+ +EDILDILISLKD++ NSLLSIEEIKAQIT+LQIATIDNPSNAVEWAMAEL+NQP
Sbjct: 301 KKIKEVEDILDILISLKDENTNSLLSIEEIKAQITDLQIATIDNPSNAVEWAMAELLNQP 360

Query: 361 EILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVN 420
           +IL+K VEELD+VVGRERLV+ESDIPKLKYLTAC RESFRLHPFS FN+PH+S SDI+V 
Sbjct: 361 KILEKVVEELDKVVGRERLVQESDIPKLKYLTACARESFRLHPFSSFNVPHISTSDIVVA 420

Query: 421 GYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGR 480
           GY IPKGSEVLLSR GLGRNPR+WEDPMRF+PERHLKD T+ ELGISEP+LRFITFTRGR
Sbjct: 421 GYFIPKGSEVLLSRSGLGRNPRVWEDPMRFDPERHLKDGTV-ELGISEPNLRFITFTRGR 480

Query: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSH 540
           RGC GSSLGTNITMMLFARLLQGF+WSL  GITK+D S+T   LS+  PL LHA+PRLSH
Sbjct: 481 RGCAGSSLGTNITMMLFARLLQGFTWSLMAGITKIDLSDTG-DLSLSKPLHLHAKPRLSH 540

Query: 541 SMYPNL 545
           SMYP+L
Sbjct: 541 SMYPSL 544

BLAST of Bhi02G001275 vs. ExPASy TrEMBL
Match: A0A1S3BQ37 (isoleucine N-monooxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103491994 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.9e-267
Identity = 460/551 (83.48%), Postives = 513/551 (93.10%), Query Frame = 0

Query: 1   MEAAF-LFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPK 60
           MEAAF +FI+QL HQ+SPIAI+K FIVF+FMAF SV+FS  YNRR+K SV++A LPPGPK
Sbjct: 1   MEAAFSIFITQLPHQNSPIAISKAFIVFLFMAFASVMFSRCYNRRKKYSVRKALLPPGPK 60

Query: 61  PWPLVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYD 120
           PWP+VGCLPT+LTNKSPTYQWIHSVMKQFNTEIA IRLGNTY+IPVTSPELAL+FL+T+D
Sbjct: 61  PWPIVGCLPTMLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTHD 120

Query: 121 SIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEA 180
           S+F+SRSSISNTVDILSRGYLTTAFSSMG+QWKKMRRILASE+L+PT LH+ML QR AEA
Sbjct: 121 SVFSSRSSISNTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAEA 180

Query: 181 DNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 240
           D LL YIF +TS+NGGGAVINVRSITQHYCGNIIR MLFNRRYYGKGREDGGPTSEEE+H
Sbjct: 181 DTLLHYIFSITSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEEH 240

Query: 241 NEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQW-KD 300
           N+ALLTILRH+NAFSISDF+PCLKPFDLDGHEKIMK ALN VRKYDEPIIDERVQQW KD
Sbjct: 241 NQALLTILRHINAFSISDFMPCLKPFDLDGHEKIMKNALNAVRKYDEPIIDERVQQWKKD 300

Query: 301 KKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQP 360
           KKI V +DILDILIS KDD+GNSLLSI+EIKAQITELQIATIDNPSNAVEWAMAELINQP
Sbjct: 301 KKIEV-KDILDILISRKDDNGNSLLSIDEIKAQITELQIATIDNPSNAVEWAMAELINQP 360

Query: 361 EILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVN 420
           E+LKK V+ELD VVG+ERLV+E DIPKLKYLTAC+RESFRLHPFSPFN+PHV   DI+VN
Sbjct: 361 EVLKKVVDELDNVVGKERLVQEYDIPKLKYLTACLRESFRLHPFSPFNVPHVPTFDIVVN 420

Query: 421 GYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGR 480
           GYLIPKGSEVLLSR+GLGRNPRIW+DP++FNPERHLKD+T+ ELG+SEP+LRFITF+RGR
Sbjct: 421 GYLIPKGSEVLLSRLGLGRNPRIWKDPIKFNPERHLKDNTV-ELGLSEPNLRFITFSRGR 480

Query: 481 RGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSH 540
           RGCVGSSLGTNIT+MLFARLLQGFSWSL PGIT++DFS+ D +  +  PLQLHA+PRLS 
Sbjct: 481 RGCVGSSLGTNITVMLFARLLQGFSWSLLPGITQIDFSKVDREGLLLNPLQLHAEPRLSR 540

Query: 541 SMYPNLIDHEP 550
           SMYP LIDHEP
Sbjct: 541 SMYPMLIDHEP 549

BLAST of Bhi02G001275 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.8e-241
Identity = 422/542 (77.86%), Postives = 478/542 (88.19%), Query Frame = 0

Query: 4   AFLFISQLAHQHSPIAITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKPWPL 63
           A LFISQL +Q+SP AITKTF +FVFMAF S +FS  Y R+RK SV Q  LPPGP PWPL
Sbjct: 3   ALLFISQLTYQNSPTAITKTFTLFVFMAFISAIFSQCY-RKRKHSVLQ--LPPGPNPWPL 62

Query: 64  VGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFA 123
           VGCLP +L  KSP Y+WIH+VMKQF+TEIA IRLGNTY++PVTSPELA++FLK +DS+FA
Sbjct: 63  VGCLPAMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVMPVTSPELAVEFLKRHDSVFA 122

Query: 124 SRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLL 183
           SRS+ISNTV++LSRG LTTAFS +G+QWKKMRRILASE+  P++LHQMLG RT EAD LL
Sbjct: 123 SRSTISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTLL 182

Query: 184 CYIFILTSK---NGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHN 243
            YIF LT+    N  GAV+NVRS+TQHYCGNIIRRMLFN+RYYGKGREDGGP+ EEE+HN
Sbjct: 183 RYIFSLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEEEHN 242

Query: 244 EALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDKK 303
           +ALLTILRHVNAFSISDF+PCLKPFDLDGHEKIMK+AL  + KYDEPII+ERVQQWKD K
Sbjct: 243 QALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWKDGK 302

Query: 304 IRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEI 363
               EDILDILIS KD +G SLL+I+EIKAQITELQIATIDNPSNAVEW MAEL+NQP+I
Sbjct: 303 REGAEDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQPKI 362

Query: 364 LKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGY 423
           L+KA+EELD VVGRERLV+ESDIPKLKYLTAC RESFRLHPFSPFNIPHVSN+DI+V GY
Sbjct: 363 LQKAIEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVAGY 422

Query: 424 LIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRG 483
            IPKGSEV+LSR GLGRNPRIWEDPMRF+PERHLKD+T+ ELG+SEP+LRFI+FTRGRRG
Sbjct: 423 FIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDETV-ELGLSEPNLRFISFTRGRRG 482

Query: 484 CVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSM 543
           CVGSSLGTNITMML  RLLQGFSWSLP G TK++FSE D +LS+P PL LHA+PRL H M
Sbjct: 483 CVGSSLGTNITMMLLGRLLQGFSWSLPFGTTKIEFSEAD-ELSLPKPLHLHAKPRLPHIM 539

BLAST of Bhi02G001275 vs. ExPASy TrEMBL
Match: A0A6J1D6G3 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017495 PE=3 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 8.4e-238
Identity = 420/543 (77.35%), Postives = 476/543 (87.66%), Query Frame = 0

Query: 4   AFLFISQLAHQHSPI-AITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPPGPKPWP 63
           AFL I QL++Q+SP  AI KTF VF+FMAF S +FS  Y R+RK SV Q  LPPGPKPWP
Sbjct: 3   AFLIIWQLSYQNSPTGAINKTFTVFLFMAFISAIFSQCY-RKRKHSVLQ--LPPGPKPWP 62

Query: 64  LVGCLPTILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIF 123
           LVGCLP++L  KSP Y+WIH+VMKQF+TEIA IRLGNTY+IPVTSPELA++FLK +DS+F
Sbjct: 63  LVGCLPSMLATKSPKYKWIHAVMKQFDTEIACIRLGNTYVIPVTSPELAVEFLKKHDSVF 122

Query: 124 ASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNL 183
           ASRS++SNTV++L+RG LTTAFS +G+QWKKMRRILASE+  P++LHQMLG RT EAD L
Sbjct: 123 ASRSTVSNTVNVLTRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTL 182

Query: 184 LCYIFILT---SKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDH 243
           L YIF LT   + N GGAV+NV S+TQHYCGNIIRRMLFNRRYYGKGREDGGP+ EEE+H
Sbjct: 183 LRYIFSLTTTNTNNEGGAVVNVGSVTQHYCGNIIRRMLFNRRYYGKGREDGGPSFEEEEH 242

Query: 244 NEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQWKDK 303
           N+ALLTILRHVNAFSISDF+PCLKPFDLDGHEKIMK+AL  + KYDEPII+ERVQQWKD 
Sbjct: 243 NQALLTILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWKDG 302

Query: 304 KIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPE 363
           K    EDILDILIS+KD +G SLL+IEEIKAQITELQIATIDNPSNAVEW MAEL+NQP+
Sbjct: 303 KREGAEDILDILISIKDGNGKSLLTIEEIKAQITELQIATIDNPSNAVEWTMAELLNQPK 362

Query: 364 ILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNG 423
           IL+KA+EELD VVGR RLV+ESDIPKLKYLTA +RESFRLHPFSPFNIPHVSN+DIIV G
Sbjct: 363 ILQKAIEELDRVVGRGRLVQESDIPKLKYLTASVRESFRLHPFSPFNIPHVSNADIIVAG 422

Query: 424 YLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRR 483
           Y IPKGSEV+LSR GLGRNPRIWEDPMRF+PERHLKD T+  LG+SEPSLRFI+FTRGRR
Sbjct: 423 YFIPKGSEVILSRSGLGRNPRIWEDPMRFDPERHLKDGTVA-LGLSEPSLRFISFTRGRR 482

Query: 484 GCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHS 543
           GCVGSSLGTNITM L  RLLQGFSWSLP G TK++FSE D +LS+P PL LHA+PRL H 
Sbjct: 483 GCVGSSLGTNITMTLLGRLLQGFSWSLPFGTTKIEFSEAD-ELSLPKPLHLHAKPRLPHI 540

BLAST of Bhi02G001275 vs. ExPASy TrEMBL
Match: A0A5A7UP06 (Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00660 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.7e-236
Identity = 408/481 (84.82%), Postives = 451/481 (93.76%), Query Frame = 0

Query: 70  ILTNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFLKTYDSIFASRSSIS 129
           +LTNKSPTYQWIHSVMKQFNTEIA IRLGNTY+IPVTSPELAL+FL+T+DS+F+SRSSIS
Sbjct: 1   MLTNKSPTYQWIHSVMKQFNTEIACIRLGNTYVIPVTSPELALEFLRTHDSVFSSRSSIS 60

Query: 130 NTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQRTAEADNLLCYIFIL 189
           NTVDILSRGYLTTAFSSMG+QWKKMRRILASE+L+PT LH+ML QR AEAD LL YIF +
Sbjct: 61  NTVDILSRGYLTTAFSSMGDQWKKMRRILASEILSPTMLHRMLEQRIAEADTLLHYIFSI 120

Query: 190 TSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSEEEDHNEALLTILRH 249
           TS+NGGGAVINVRSITQHYCGNIIR MLFNRRYYGKGREDGGPTSEEE+HN+ALLTILRH
Sbjct: 121 TSENGGGAVINVRSITQHYCGNIIRTMLFNRRYYGKGREDGGPTSEEEEHNQALLTILRH 180

Query: 250 VNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQW-KDKKIRVIEDIL 309
           +NAFSISDF+PCLKPFDLDGHEKIMK ALN VRKYDEPIIDERVQQW KDKKI V +DIL
Sbjct: 181 INAFSISDFMPCLKPFDLDGHEKIMKNALNAVRKYDEPIIDERVQQWKKDKKIEV-KDIL 240

Query: 310 DILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAELINQPEILKKAVEEL 369
           DILIS KDD+GNSLLSI+EIKAQITELQIATIDNPSNAVEWAMAELINQPE+LKK V+EL
Sbjct: 241 DILISRKDDNGNSLLSIDEIKAQITELQIATIDNPSNAVEWAMAELINQPEVLKKVVDEL 300

Query: 370 DEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSDIIVNGYLIPKGSEV 429
           D VVG+ERLV+E DIPKLKYLTAC+RESFRLHPFSPFN+PHV   DI+VNGYLIPKGSEV
Sbjct: 301 DNVVGKERLVQEYDIPKLKYLTACLRESFRLHPFSPFNVPHVPTFDIVVNGYLIPKGSEV 360

Query: 430 LLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITFTRGRRGCVGSSLGT 489
           LLSR+GLGRNPRIW+DP++FNPERHLKD+T+ ELG+SEP+LRFITF+RGRRGCVGSSLGT
Sbjct: 361 LLSRLGLGRNPRIWKDPIKFNPERHLKDNTV-ELGLSEPNLRFITFSRGRRGCVGSSLGT 420

Query: 490 NITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQPRLSHSMYPNLIDHE 549
           NIT+MLFARLLQGFSWSL PGIT++DFS+ D +  +  PLQLHA+PRLS SMYP LIDHE
Sbjct: 421 NITVMLFARLLQGFSWSLLPGITQIDFSKVDREGLLLNPLQLHAEPRLSRSMYPMLIDHE 479

BLAST of Bhi02G001275 vs. ExPASy TrEMBL
Match: A0A5D3DVF9 (Isoleucine N-monooxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G00730 PE=3 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 5.1e-235
Identity = 410/554 (74.01%), Postives = 475/554 (85.74%), Query Frame = 0

Query: 1   MEAAFLFISQLAHQHSPIA----ITKTFIVFVFMAFTSVLFSHFYNRRRKLSVQQAPLPP 60
           ME A +F  QL +Q+SP+A    +  +F++F+FMAF S+LF   YNR  K S  Q PLPP
Sbjct: 1   MEVASIFTGQLIYQNSPVAMISNLQPSFVMFLFMAFLSILFFQCYNRTEKHSNLQPPLPP 60

Query: 61  GPKPWPLVGCLPTIL-TNKSPTYQWIHSVMKQFNTEIASIRLGNTYIIPVTSPELALQFL 120
           GPKPWPL+GCLP +L +N S T++WIHS+MKQFN EIASIRL NTY+IPV SPELAL+FL
Sbjct: 61  GPKPWPLLGCLPAMLSSNNSSTHEWIHSIMKQFNAEIASIRLRNTYVIPVASPELALEFL 120

Query: 121 KTYDSIFASRSSISNTVDILSRGYLTTAFSSMGNQWKKMRRILASEVLNPTSLHQMLGQR 180
           KTYDS+F SRSS SN VD+L+ G ++T  S  G QW+KM+RIL SE+LNP++LH+MLGQR
Sbjct: 121 KTYDSVFGSRSSFSNDVDMLTGGCVSTILSPTGPQWRKMKRILTSEILNPSTLHRMLGQR 180

Query: 181 TAEADNLLCYIFILTSKNGGGAVINVRSITQHYCGNIIRRMLFNRRYYGKGREDGGPTSE 240
           TAEAD LL YIF  T KNGGGAVINVRSITQHYCGNIIRR++FNRRYYGKGREDGGPT E
Sbjct: 181 TAEADALLHYIFNQTCKNGGGAVINVRSITQHYCGNIIRRIVFNRRYYGKGREDGGPTFE 240

Query: 241 EEDHNEALLTILRHVNAFSISDFVPCLKPFDLDGHEKIMKKALNVVRKYDEPIIDERVQQ 300
           EE+HN+ALLTI+RHVN+FSISDF+PCLKP DLDGH+K MK ALNV+RKYDE II ERVQQ
Sbjct: 241 EEEHNQALLTIVRHVNSFSISDFMPCLKPLDLDGHQKNMKNALNVLRKYDEHIIKERVQQ 300

Query: 301 WK-DKKIRVIEDILDILISLKDDHGNSLLSIEEIKAQITELQIATIDNPSNAVEWAMAEL 360
           WK D+KI+ +EDILDILISL DD+GNSLLSIEEIK QI ++Q+ATIDNPSNAVEWAMAEL
Sbjct: 301 WKNDEKIKGVEDILDILISLTDDNGNSLLSIEEIKNQIMDIQLATIDNPSNAVEWAMAEL 360

Query: 361 INQPEILKKAVEELDEVVGRERLVEESDIPKLKYLTACIRESFRLHPFSPFNIPHVSNSD 420
           +NQP++LKKA+EELD+VVGRERLV+ESDI  LKYLTAC RESFRLHPFSPFN+ HVSNSD
Sbjct: 361 LNQPKVLKKAIEELDKVVGRERLVQESDISNLKYLTACARESFRLHPFSPFNVAHVSNSD 420

Query: 421 IIVNGYLIPKGSEVLLSRIGLGRNPRIWEDPMRFNPERHLKDDTMTELGISEPSLRFITF 480
           I+V GY IPKGSEVLLSR+GLGRNPRIWEDPM+FNPERHLKD T+ ELGISEP+LRFITF
Sbjct: 421 IVVAGYFIPKGSEVLLSRLGLGRNPRIWEDPMKFNPERHLKDGTI-ELGISEPNLRFITF 480

Query: 481 TRGRRGCVGSSLGTNITMMLFARLLQGFSWSLPPGITKMDFSETDHQLSVPTPLQLHAQP 540
           TRGRRGC GSSLGTNITMMLFARLLQGFSW+  P  TK D  +T ++LS+  PL LHA+P
Sbjct: 481 TRGRRGCPGSSLGTNITMMLFARLLQGFSWTSLPKFTKTDLPQT-NELSLLKPLHLHAKP 540

Query: 541 RLSHSMYPNLIDHE 549
           RLSH MY + I H+
Sbjct: 541 RLSHDMYQSFIGHQ 552