Bhi02G001263 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G001263
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Isoleucine N-monooxygenase 1
Location	chr2: 40215925 .. 40216526 (+)
RNA-Seq Expression	Bhi02G001263
Synteny	Bhi02G001263

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGTTGAGATATTGAGCCGCAGATATCTTAGTGCAGTTCTTTCACCCATGGGAGGTCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCATGTTATGAATCCTTCAACTCACCATCGACTGCTTGGCCAAAGAACAAAGGAAGCTGAACTCTCTTACGCTACATTTTCAGCCTCACTGAAAATGGTGAAGCGATCAACATAAGAAGCATTGCACAACATTATTGCGGTAACATTATTAGAAGAATGGTGTTTAATAGAAGATATTATGGAAAAGGGAGGGAAGATGGGGGGCCATCTTTTGAAGAAGAGAACCATAATCAAGCAATGTTTACATTTCTAACGTATCTTAATGCATTTTTCGTATAAAATTTTATGCCTTGTTTGAAGCCATTTGATCTAGATGGACACGAGAAGATAGTAAAAAAGGCTTTTAAAGTCATAAGAGATCTTGACGAACCCATTATAGACAAAAGAGTGCAAGAATTGAGAGATGAGAAGAAAAAGGAGGTGGAGGACATTCTTGATATCCTTATCTCACTCAAAAATGAGAATGGTAAATCGTTGTTGTCAATCGAAGAGATTAAGGCTCAAGTCACG

mRNA sequence

ATGACTGTTGAGATATTGAGCCGCAGATATCTTAGTGCAGTTCTTTCACCCATGGGAGGTCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCATGTTATGAATCCTTCAACTCACCATCGACTGCTTGGCCAAAGAACAAAGGAAGCTGAACTCTCTTACGCTACATTTTCAGCCTCACTGAAAATGCCATTTGATCTAGATGGACACGAGAAGATAGTAAAAAAGGCTTTTAAAGTCATAAGAGATCTTGACGAACCCATTATAGACAAAAGAGTGCAAGAATTGAGAGATGAGAAGAAAAAGGAGGTGGAGGACATTCTTGATATCCTTATCTCACTCAAAAATGAGAATGGTAAATCGTTGTTGTCAATCGAAGAGATTAAGGCTCAAGTCACG

Coding sequence (CDS)

ATGACTGTTGAGATATTGAGCCGCAGATATCTTAGTGCAGTTCTTTCACCCATGGGAGGTCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCATGTTATGAATCCTTCAACTCACCATCGACTGCTTGGCCAAAGAACAAAGGAAGCTGAACTCTCTTACGCTACATTTTCAGCCTCACTGAAAATGCCATTTGATCTAGATGGACACGAGAAGATAGTAAAAAAGGCTTTTAAAGTCATAAGAGATCTTGACGAACCCATTATAGACAAAAGAGTGCAAGAATTGAGAGATGAGAAGAAAAAGGAGGTGGAGGACATTCTTGATATCCTTATCTCACTCAAAAATGAGAATGGTAAATCGTTGTTGTCAATCGAAGAGATTAAGGCTCAAGTCACG

Protein sequence

MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAELSYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILISLKNENGKSLLSIEEIKAQVT

Homology

[Close]

BLAST of Bhi02G001263 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 79.0 bits (193), Expect = 3.3e-15
Identity = 60/207 (28.99%), Postives = 89/207 (43.00%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE--------- 60
           M  E  SR YL+  + P G QWKKMRR++ SHV +  +   +L +RT+EA+         
Sbjct: 109 MGTEYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNR 168

Query: 61  ---------------LSYATFSASL--KMPF----------------------------- 120
                          L+   +S ++  KM F                             
Sbjct: 169 SVKNRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTV 228

Query: 121 -------------------DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVED 134
                              DL+GHEK+V  A + +   ++P +D+R+ + R+ K KE +D
Sbjct: 229 LTHLYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDERLMQWRNGKMKEPQD 288

[Close]

BLAST of Bhi02G001263 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-13
Identity = 59/209 (28.23%), Postives = 87/209 (41.63%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEA---------- 60
           M  E  SR YL+  +   G QWKKMRR++ SHV +  +   +L +RT+EA          
Sbjct: 117 MGTEYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFKLMLEKRTEEADNLVRYINNR 176

Query: 61  ---------------------------------------------------ELSYA---- 120
                                                              E+ +     
Sbjct: 177 CVKNRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSEDESGPGSEEIEHVESLF 236

Query: 121 -------TFSASLKMPF----DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEV 134
                   F+ S  +P+    DL+GHEK+V  A + +   ++P +D+R+ + R+ K KE 
Sbjct: 237 TVLTHLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPFVDERLMQWRNGKMKEP 296

[Close]

BLAST of Bhi02G001263 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 67.4 bits (163), Expect = 9.9e-12
Identity = 51/198 (25.76%), Postives = 89/198 (44.95%), Query Frame = 0


Query: 4   EILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE------------ 63
           +ILS  Y + V++P G Q+KKMR+++++ ++ P+ H  L   R +E +            
Sbjct: 135 KILSNGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKN 194

Query: 64  --------------------LSYAT---------------------------------FS 123
                               L + T                                 F 
Sbjct: 195 SEPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFC 254

Query: 124 ASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILISL 133
            S  +P     DL+GHEKI++++  ++    +PIID+R++  R+ K+ ++ED LDI IS+
Sbjct: 255 ISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISI 314

[Close]

BLAST of Bhi02G001263 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-11
Identity = 51/198 (25.76%), Postives = 90/198 (45.45%), Query Frame = 0


Query: 4   EILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKE-------------- 63
           +ILS  Y + V++P G Q+KKMR+++++ ++ P+ H  L  +R++E              
Sbjct: 133 KILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQKRSEENDHLTAWVYNMVKN 192

Query: 64  ------------------AELSYAT---------------------------------FS 123
                              +L + T                                 F 
Sbjct: 193 SGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFC 252

Query: 124 ASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILISL 133
            S  +P     DL+GHEKI++++  ++    +PIID+R++  R+ K+ ++ED LDI IS+
Sbjct: 253 ISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISI 312

[Close]

BLAST of Bhi02G001263 vs. TAIR 10
Match: AT1G16410.2 (cytochrome p450 79f1 )

HSP 1 Score: 55.8 bits (133), Expect = 3.0e-08
Identity = 33/68 (48.53%), Postives = 48/68 (70.59%), Query Frame = 0


Query: 65  FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDE-KKKEVEDILDILISLKNENGKSLL 124
           +++DG EK V +   ++R  + PIID+RVQ  R+E  K  VED LD  I+LK++NGK L+
Sbjct: 255 WNVDGQEKRVTENCNIVRSYNNPIIDERVQLWREEGGKAAVEDWLDTFITLKDQNGKYLV 314

Query: 125 SIEEIKAQ 132
           + +EIKAQ
Sbjct: 315 TPDEIKAQ 322

[Close]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-21
Identity = 71/204 (34.80%), Postives = 97/204 (47.55%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE--------- 60
           M  + LS  YL+ V+ P G QW+KMRR+LV+   NPS  H LLG+R +EA+         
Sbjct: 120 MATKTLSSGYLTTVVGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQ 179

Query: 61  ---------------------------------------------------------LSY 120
                                                                    L+Y
Sbjct: 180 CSANQNGAVVNVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTY 239

Query: 121 A-TFSASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILD 134
           A  F  S  +P    FD+DGHEK V+KA  +++   EPI+++R+QE RD K+ E +D+LD
Sbjct: 240 AYAFCVSDYLPWLRVFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLD 299

[Close]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.8e-16
Identity = 61/205 (29.76%), Postives = 93/205 (45.37%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE--------- 60
           M+ +I S  +++ VL P G QWKKM+R+LV+++++P  H  LLG+R +EA+         
Sbjct: 119 MSTDIASDGFITTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNK 178

Query: 61  -----------------------------------------------------------L 120
                                                                      L
Sbjct: 179 CCKDVNDGPGLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTIL 238

Query: 121 SYA-TFSASLKMPF----DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDI 133
            Y   FS S  +PF    DLDGH   + KA +++R   +PIID R+++  D  K   ED+
Sbjct: 239 KYVYAFSISDFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDL 298

[Close]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot
Match: Q9M7B7 (Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.5e-15
Identity = 59/200 (29.50%), Postives = 92/200 (46.00%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE--------- 60
           +  + +S  YL+ ++ P   QWKKMR++L S +++P+ H  L  +R +EA+         
Sbjct: 132 LCAKTMSGGYLTTIVVPYNDQWKKMRKVLTSEIISPARHKWLHDKRAEEADQLVFYINNQ 191

Query: 61  ------------------------------------------------------LSYA-T 120
                                                                 L Y   
Sbjct: 192 YKSNKNVNVRIAARHYGGNVIRKMMFSKRYFGKGMPDGGPGPEEIMHVDAIFTALKYLYG 251

Query: 121 FSASLKMPF----DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 133
           F  S  +PF    DLDG EKIV  A K IRDL  P+I++R+Q+ R  ++KE+ED+LD+ I
Sbjct: 252 FCISDYLPFLEGLDLDGQEKIVLNANKTIRDLQNPLIEERIQQWRSGERKEMEDLLDVFI 311

[Close]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 5.5e-15
Identity = 61/206 (29.61%), Postives = 92/206 (44.66%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE--------- 60
           M+ +I S  +L+ VL P G QWKKM+R+LV+++++P  H  LLG+R +EA+         
Sbjct: 119 MSTDIASDGFLTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNK 178

Query: 61  -----------------------------------------------------------L 120
                                                                      L
Sbjct: 179 CCKDVNDGPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTIL 238

Query: 121 SYA-TFSASLKMPF----DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDI 134
            Y   FS S  +PF    DLDGH   + KA  +++   +PII  R+++  D  K   ED+
Sbjct: 239 KYVYAFSISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDL 298

[Close]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 81.6 bits (200), Expect = 7.2e-15
Identity = 65/201 (32.34%), Postives = 89/201 (44.28%), Query Frame = 0


Query: 4   EILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE------------ 63
           E  S  Y +AVLSP G QWKKMRR+L S ++ PS H  L  +RT EA+            
Sbjct: 145 ETFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRHAWLHDKRTDEADNLTRYVYNLATK 204

Query: 64  ---------------------------------------------------------LSY 123
                                                                    L Y
Sbjct: 205 AATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQADGGPGPMEVLHMDAVFTSLGLLY 264

Query: 124 ATFSASLKMPF----DLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDI 132
           A F  S  +P+    DLDGHEKIVK+A   +  L + +ID R ++ +  +++E+ED LD+
Sbjct: 265 A-FCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDTVIDDRWRQWKSGERQEMEDFLDV 324

[Close]

BLAST of Bhi02G001263 vs. NCBI nr
Match: KAA0067056.1 (isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 149.4 bits (376), Expect = 2.1e-32
Identity = 97/201 (48.26%), Postives = 112/201 (55.72%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAEL-------- 60
           MT EI+S  YLS VLSPMG QWKKMR+ILVS V+NP T H+++GQRT EA++        
Sbjct: 112 MTAEIVSNGYLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGL 171

Query: 61  --------------------------------------------------------SYAT 120
                                                                       
Sbjct: 172 TRNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNA 231

Query: 121 FSASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           FS S  +P    FDLDGHEKIVK+A KVIR+ DEPII++RVQ+ RD KKK+ EDILDILI
Sbjct: 232 FSISEFIPLLKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDILI 291

[Close]

BLAST of Bhi02G001263 vs. NCBI nr
Match: XP_011655052.1 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protein Csa_005885 [Cucumis sativus])

HSP 1 Score: 146.7 bits (369), Expect = 1.4e-31
Identity = 98/201 (48.76%), Postives = 110/201 (54.73%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAEL-------- 60
           MT EI+S  YLS VLSPMG QWKKMR+IL S V+N ST HR+LGQRT EA++        
Sbjct: 112 MTAEIVSNGYLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFGL 171

Query: 61  --------------------------------------------------------SYAT 120
                                                                       
Sbjct: 172 TKNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVNA 231

Query: 121 FSASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           FS S  +P    FDLDGH KIVK+A KVIR+ DEPII++RVQE  D KKK+VEDILDILI
Sbjct: 232 FSISEFIPLLKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVEDILDILI 291

[Close]

BLAST of Bhi02G001263 vs. NCBI nr
Match: KAA0040213.1 (phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 139.4 bits (350), Expect = 2.2e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 47  TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 106

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 107 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKRRENEGPSLEEEDYNQALLTILMHLYSF 166

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 167 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 226

[Close]

BLAST of Bhi02G001263 vs. NCBI nr
Match: XP_008450287.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 139.4 bits (350), Expect = 2.2e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 135 TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 194

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 195 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSF 254

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 255 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 314

[Close]

BLAST of Bhi02G001263 vs. NCBI nr
Match: TYK07875.1 (phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 139.4 bits (350), Expect = 2.2e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 47  TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 106

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 107 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSF 166

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 167 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 226

[Close]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL
Match: A0A5A7VKT9 (Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00680 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.0e-32
Identity = 97/201 (48.26%), Postives = 112/201 (55.72%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAEL-------- 60
           MT EI+S  YLS VLSPMG QWKKMR+ILVS V+NP T H+++GQRT EA++        
Sbjct: 112 MTAEIVSNGYLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFGL 171

Query: 61  --------------------------------------------------------SYAT 120
                                                                       
Sbjct: 172 TRNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVNA 231

Query: 121 FSASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           FS S  +P    FDLDGHEKIVK+A KVIR+ DEPII++RVQ+ RD KKK+ EDILDILI
Sbjct: 232 FSISEFIPLLKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDILI 291

[Close]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL
Match: A0A0A0KM68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G223620 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.7e-32
Identity = 98/201 (48.76%), Postives = 110/201 (54.73%), Query Frame = 0


Query: 1   MTVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAEL-------- 60
           MT EI+S  YLS VLSPMG QWKKMR+IL S V+N ST HR+LGQRT EA++        
Sbjct: 112 MTAEIVSNGYLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFGL 171

Query: 61  --------------------------------------------------------SYAT 120
                                                                       
Sbjct: 172 TKNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVNA 231

Query: 121 FSASLKMP----FDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           FS S  +P    FDLDGH KIVK+A KVIR+ DEPII++RVQE  D KKK+VEDILDILI
Sbjct: 232 FSISEFIPLLKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVEDILDILI 291

[Close]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL
Match: A0A5D3C8R5 (Phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1933G00200 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 47  TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 106

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 107 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSF 166

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 167 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 226

[Close]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL
Match: A0A5A7TAV9 (Phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2228G00080 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 47  TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 106

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 107 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKRRENEGPSLEEEDYNQALLTILMHLYSF 166

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 167 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 226

[Close]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL
Match: A0A1S3BNA6 (phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-29
Identity = 91/201 (45.27%), Postives = 109/201 (54.23%), Query Frame = 0


Query: 2   TVEILSRRYLSAVLSPMGGQWKKMRRILVSHVMNPSTHHRLLGQRTKEAE---------- 61
           T EILS  +LS VLSPMG QWKKM+RIL SHV+NPST H++ GQR KEA+          
Sbjct: 135 TTEILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHA 194

Query: 62  -----------------------------------------------------------L 121
                                                                       
Sbjct: 195 SRCEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSF 254

Query: 122 SYATFSASLKMPFDLDGHEKIVKKAFKVIRDLDEPIIDKRVQELRDEKKKEVEDILDILI 134
           S + + A LK PFDL GH+KI++KA KVI + D PII++RVQE RD KKKEV+DILDILI
Sbjct: 255 SISEYIACLK-PFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILI 314

The following BLAST results are available for this feature:

BLAST of Bhi02G001263 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05260.1	3.3e-15	28.99	cytochrome p450 79a2	[more]
AT5G35917.1	1.4e-13	28.23	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT2G22330.1	9.9e-12	25.76	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT4G39950.1	1.7e-11	25.76	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT1G16410.2	3.0e-08	48.53	cytochrome p450 79f1	[more]

BLAST of Bhi02G001263 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q605	1.8e-21	34.80	Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV...	[more]
Q6J541	3.8e-16	29.76	Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1	[more]
Q9M7B7	5.5e-15	29.50	Valine N-monooxygenase 2 OS=Manihot esculenta OX=3983 GN=CYP79D2 PE=1 SV=1	[more]
Q6J540	5.5e-15	29.61	Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1	[more]
Q43135	7.2e-15	32.34	Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3	[more]

BLAST of Bhi02G001263 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0067056.1	2.1e-32	48.26	isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N...	[more]
XP_011655052.1	1.4e-31	48.76	isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protei...	[more]
KAA0040213.1	2.2e-29	45.27	phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008450287.1	2.2e-29	45.27	PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo]	[more]
TYK07875.1	2.2e-29	45.27	phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo var. makuwa]	[more]

BLAST of Bhi02G001263 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VKT9	1.0e-32	48.26	Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0KM68	6.7e-32	48.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G223620 PE=4 SV=1	[more]
A0A5D3C8R5	1.1e-29	45.27	Phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7TAV9	1.1e-29	45.27	Phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BNA6	1.1e-29	45.27	phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 97..117
None	No IPR available	PANTHER	PTHR24298:SF675	PHENYLALANINE N-MONOOXYGENASE	coord: 1..51 coord: 61..133
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..51
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 61..133
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..133 e-value: 5.6E-8 score: 34.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 14..132
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 65..132 e-value: 1.3E-5 score: 24.2

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001263	Bhi02M001263	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen