Bhi02G001027 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G001027
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	GTP-binding protein 8 isoform X2
Location	chr2: 29166075 .. 29167244 (-)
RNA-Seq Expression	Bhi02G001027
Synteny	Bhi02G001027

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCAAGAGCCCAGCTTCAGGCTTAGGCTCAAGAAGAAACTCAAACTTGCATTTGGAAATTTAAGAATTCGAAAAACTTTGATAGAAGCACAGGGGATAGTGTTGAAAAAGCTACTTGAAAGGGAAAATTTAGGCCTACAATTACTAATGAAAAAGACAGGGTATATGGTGGCCAAAATAGTGATATGAAGGTCACTTCATTCATTGATGTTGCGAAATCCAAGAGGAAATCTCTTTTGAGAAGACGACACGATGTTGAAGTTGTTGGAATGCCTTCAGTTTTGGCTTCTAAGACTTCAAATCGCTCTGTGAAAAAGCAAGACATTGGGGTAAGAAGTTCGAAAGTATTATATGAAAAAAGAAATAGTAGAGGGGAAAAGGCCACAATGTAGAATAGTAAGGAAGACACATTTCAAAAACACCAAGGATTCAAGCAACAAATTGGATTCTACTGAGGAAAGAGTTTCTAAGAAAAGAAGTGAAAATGGTGATTCAAAGTTCCTAAGGCTCCATTTTGTAACCATTTTGTTTTTTAAAATTAAATTTATGGACATTATTTCCATCTCTAAGTTTCTTCCTTTGTTATTTACTTTTCACCAATAGTTTAAAAAATCAAGCCAAATTTTAAGAACAAAAAAAGTACTTTCAAAAGGTTATTTTTATTTTTCGAATTTGGCTAAGAATTCAACTATTGTACTTGAAAAGATGCAAATCATGCTAAGAAATGTGGATGTAATAAACTTAATTTTCAAGAAACACAAATAAAAAATCAAATGATTACCAAATGGGACTTAAATAATCAACCGAAAGAGAGGAAACAGTTAATTCATATCGATCCATACGATCTCTCTAATAAGCGGTTGGATGATGGGATAATAATTGATGGTGAGTTGATATCTATTTAGGATGTAATTGAACTACTTTATTTTTCAATAGGCTAAGTTTAATGATATATTGTCTCTCGTCTGTTCGCTTGCATTCCTATTTGAGTTCATTTTAGAGGCTCTTCCTCTTCCTTTAATTCCAGAAAATGCCAGGCAGGAGAAAGCAAACCCAAAGAAAAAGGATGAGATGTCAAAGAATGCTCAGTTTCGAGCAATACAACCTAATAAATATATCATTTCATTCGTAGAAGAGAATGTAATTTGCATAGCTATGTGTTGTTAA

mRNA sequence

ATGTCCCAAGAGCCCAGCTTCAGGCTTAGGCTCAAGAAGAAACTCAAACTTGCATTTGGAAATTTAAGAATTCGAAAAACTTTGATAGAAGCACAGGGGATAGTGTTGAAAAAGCTACTTGAAAGGGAAAATTTAGGCCTACAATTACTAATGAAAAAGACAGGGTATATGGTCACTTCATTCATTGATGTTGCGAAATCCAAGAGGAAATCTCTTTTGAGAAGACGACACGATGTTGAAGTTGTTGGAATGCCTTCAGTTTTGGCTTCTAAGACTTCAAATCGCTCTGTGAAAAAGCAAGACATTGGGGCTAAGTTTAATGATATATTGTCTCTCGTCTGTTCGCTTGCATTCCTATTTGAGTTCATTTTAGAGGCTCTTCCTCTTCCTTTAATTCCAGAAAATGCCAGGCAGGAGAAAGCAAACCCAAAGAAAAAGGATGAGATGTCAAAGAATGCTCAGTTTCGAGCAATACAACCTAATAAATATATCATTTCATTCGTAGAAGAGAATGTAATTTGCATAGCTATGTGTTGTTAA

Coding sequence (CDS)

ATGTCCCAAGAGCCCAGCTTCAGGCTTAGGCTCAAGAAGAAACTCAAACTTGCATTTGGAAATTTAAGAATTCGAAAAACTTTGATAGAAGCACAGGGGATAGTGTTGAAAAAGCTACTTGAAAGGGAAAATTTAGGCCTACAATTACTAATGAAAAAGACAGGGTATATGGTCACTTCATTCATTGATGTTGCGAAATCCAAGAGGAAATCTCTTTTGAGAAGACGACACGATGTTGAAGTTGTTGGAATGCCTTCAGTTTTGGCTTCTAAGACTTCAAATCGCTCTGTGAAAAAGCAAGACATTGGGGCTAAGTTTAATGATATATTGTCTCTCGTCTGTTCGCTTGCATTCCTATTTGAGTTCATTTTAGAGGCTCTTCCTCTTCCTTTAATTCCAGAAAATGCCAGGCAGGAGAAAGCAAACCCAAAGAAAAAGGATGAGATGTCAAAGAATGCTCAGTTTCGAGCAATACAACCTAATAAATATATCATTTCATTCGTAGAAGAGAATGTAATTTGCATAGCTATGTGTTGTTAA

Protein sequence

MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKKTGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDILSLVCSLAFLFEFILEALPLPLIPENARQEKANPKKKDEMSKNAQFRAIQPNKYIISFVEENVICIAMCC

Homology

[Close]

BLAST of Bhi02G001027 vs. TAIR 10
Match: AT5G58370.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.5e-04
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 145 KKDEMSKNAQFRAIQPNKYIISFVEENVI 174
           K  EMSKNAQFRAIQP+  I+S+VEEN++
Sbjct: 199 KSVEMSKNAQFRAIQPSHSILSYVEENLL 227

[Close]

BLAST of Bhi02G001027 vs. TAIR 10
Match: AT5G58370.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.5e-04
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 145 KKDEMSKNAQFRAIQPNKYIISFVEENVI 174
           K  EMSKNAQFRAIQP+  I+S+VEEN++
Sbjct: 199 KSVEMSKNAQFRAIQPSHSILSYVEENLL 227

[Close]

BLAST of Bhi02G001027 vs. NCBI nr
Match: XP_038879328.1 (uncharacterized protein LOC120071244 [Benincasa hispida] >XP_038879329.1 uncharacterized protein LOC120071244 [Benincasa hispida] >XP_038879330.1 uncharacterized protein LOC120071244 [Benincasa hispida] >XP_038879331.1 uncharacterized protein LOC120071244 [Benincasa hispida])

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-18
Identity = 90/256 (35.16%), Postives = 115/256 (44.92%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLER-------ENLGLQLLM-- 60
           MS+EPS  LRLKKKLKLA G  +  K    + G  ++K   R       +N+    +   
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRSTGDSVEKAARRGKLKPATDNVKRHRIYGG 60

Query: 61  KKTGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
           + +   V SF DV K KRKSL RRR D EVVGMP+VLASKTSN+S+KKQD G + + +  
Sbjct: 61  RNSDMKVNSFTDVMKFKRKSLSRRRQDDEVVGMPTVLASKTSNQSLKKQDNGVRSSKVFY 120

Query: 121 ------------------------------------------------------------ 174
                                                                       
Sbjct: 121 DKRKSRGEKDHNVEYVRKTHSKNTKDSSKTLDSTEKKSWRVTHSDPAKKRDSKKRSENGD 180

[Close]

BLAST of Bhi02G001027 vs. NCBI nr
Match: KAA0058148.1 (GTP-binding protein 8 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 95.1 bits (235), Expect = 6.4e-16
Identity = 93/292 (31.85%), Postives = 120/292 (41.10%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K    + G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRSTGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSVL SKT NRS KKQ+ G + + +  
Sbjct: 61  VDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVLPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------SLAFLF 174
                                                LS V             +L ++ 
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. NCBI nr
Match: XP_008453496.1 (PREDICTED: uncharacterized protein LOC103494192 [Cucumis melo])

HSP 1 Score: 91.7 bits (226), Expect = 7.1e-15
Identity = 93/297 (31.31%), Postives = 115/297 (38.72%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K      G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRITGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSV  SKT NRS KKQ+ G + + +  
Sbjct: 61  EDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVFPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------------ 174
                                                LS V                   
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. NCBI nr
Match: TYK28506.1 (GTP-binding protein 8 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 91.7 bits (226), Expect = 7.1e-15
Identity = 93/297 (31.31%), Postives = 115/297 (38.72%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K      G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRITGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSV  SKT NRS KKQ+ G + + +  
Sbjct: 61  EDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVFPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------------ 174
                                                LS V                   
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. NCBI nr
Match: KAG6590331.1 (hypothetical protein SDJN03_15754, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 88.6 bits (218), Expect = 6.0e-14
Identity = 49/88 (55.68%), Postives = 64/88 (72.73%), Query Frame = 0


Query: 93  SNRSVKKQDIGAKFNDILSLVCSLAF-LFEFILEALPLPLIPENARQEKANPKKKDEMSK 152
           +++S K++       D L ++  L F + E     + LPL+PENARQEKA P++KDE+S 
Sbjct: 153 TDKSKKRKRFPTDPIDCLDVIELLYFSIAELNDRCIVLPLLPENARQEKAKPEEKDEISS 212

Query: 153 NAQFRAIQPNKYIISFVEENVICIAMCC 180
           NAQFRAIQP+K IISFVE+NVICIAMCC
Sbjct: 213 NAQFRAIQPSKSIISFVEDNVICIAMCC 240

[Close]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL
Match: A0A5A7USL6 (GTP-binding protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004590 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 3.1e-16
Identity = 93/292 (31.85%), Postives = 120/292 (41.10%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K    + G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRSTGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSVL SKT NRS KKQ+ G + + +  
Sbjct: 61  VDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVLPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------SLAFLF 174
                                                LS V             +L ++ 
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL
Match: A0A5D3DXL1 (GTP-binding protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001410 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.4e-15
Identity = 93/297 (31.31%), Postives = 115/297 (38.72%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K      G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRITGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSV  SKT NRS KKQ+ G + + +  
Sbjct: 61  EDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVFPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------------ 174
                                                LS V                   
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL
Match: A0A1S3BWG6 (uncharacterized protein LOC103494192 OS=Cucumis melo OX=3656 GN=LOC103494192 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.4e-15
Identity = 93/297 (31.31%), Postives = 115/297 (38.72%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENLGLQLLMKK------- 60
           MS+EPS  LRLKKKLKLA G  +  K      G  ++K + +  L   +   K       
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSKSSKNFDRITGDSVEKAVRKGKLKPAIPNVKRHRVYGG 60

Query: 61  --TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDI-- 120
             +   VTSF DV+KSKRKSL RRRHD EV GMPSV  SKT NRS KKQ+ G + + +  
Sbjct: 61  EDSDTKVTSFTDVSKSKRKSLFRRRHDEEVSGMPSVFPSKTFNRSFKKQEYGVRSSKVSY 120

Query: 121 -------------------------------------LSLVC------------------ 174
                                                LS V                   
Sbjct: 121 EERKSRGEKDHNVEYIRKSHSKNTKDSIKTLNYTEKKLSKVFYEKRKSRGEKDHNLEYIR 180

[Close]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL
Match: A0A0A0LPR1 (EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G025170 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.6e-12
Identity = 89/297 (29.97%), Postives = 115/297 (38.72%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTLIEAQGIVLKKLLERENL--GLQLLMKKTGY-- 60
           MS+EPS  LRLKKKLKLA G  R  K    + G  ++K + +  L   +  + +   Y  
Sbjct: 1   MSEEPSSGLRLKKKLKLAVGKSRSSKNFDRSTGDSVEKAVRKGKLRPAIDNVKRHRVYGG 60

Query: 61  -----MVTSFIDVAKSKRKSLLRRRHDVEVVGMPSVLASKTSNRSVKKQDIGAKFNDIL- 120
                  TS  DV+KSKRKSL RRRHD EV  MPSVL SKT NRS KKQ+   + + +L 
Sbjct: 61  ENSDTKDTSLTDVSKSKRKSLFRRRHDEEVSEMPSVLPSKTFNRSFKKQEYAVRSSKVLY 120

Query: 121 -------------------------SLVCSLAFL---------------------FEFI- 174
                                      + +L +                       E+I 
Sbjct: 121 EKRKSRGEKDHYVECLRKSYSKNTKDSIKTLNYTEKKVSKVFYEKRKSRGEKDHNVEYIR 180

[Close]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL
Match: A0A6J1DMT7 (uncharacterized protein LOC111022011 OS=Momordica charantia OX=3673 GN=LOC111022011 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 7.4e-10
Identity = 82/264 (31.06%), Postives = 108/264 (40.91%), Query Frame = 0


Query: 1   MSQEPSFRLRLKKKLKLAFGNLRIRKTL---------IEAQGIVLKKLLERENLGLQLLM 60
           MS+EPS  LRLKKKLKLA G    RK           I   G V K   + +       +
Sbjct: 1   MSEEPSLGLRLKKKLKLAVGKAPGRKNFDRRTRDGDTIAGTGSVEKAARKAKLRPATAHV 60

Query: 61  KK--------TGYMVTSFIDVAKSKRKSLLRRRHDVEVVGMPS----VLASKTSNRSVKK 120
           K+        +   VT F DVA  +RKSL RRR+D EVVG         AS     S+KK
Sbjct: 61  KRQRIYGGQDSDVKVTPFTDVATHRRKSLSRRRNDAEVVGKSRDESLNHASLQGKFSLKK 120

Query: 121 QDIGAKFNDIL-----------------------SLVCSLAFL----------------- 174
           Q+ G +   +                        + + S   L                 
Sbjct: 121 QENGVRSPKVSYEKGKIREGKDYNVESTRKRFSENTIDSSKTLDSTENKSWGVTPDPAKR 180

The following BLAST results are available for this feature:

BLAST of Bhi02G001027 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58370.1	3.5e-04	68.97	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G58370.2	3.5e-04	68.97	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

BLAST of Bhi02G001027 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi02G001027 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879328.1	1.4e-18	35.16	uncharacterized protein LOC120071244 [Benincasa hispida] >XP_038879329.1 unchara...	[more]
KAA0058148.1	6.4e-16	31.85	GTP-binding protein 8 isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008453496.1	7.1e-15	31.31	PREDICTED: uncharacterized protein LOC103494192 [Cucumis melo]	[more]
TYK28506.1	7.1e-15	31.31	GTP-binding protein 8 isoform X2 [Cucumis melo var. makuwa]	[more]
KAG6590331.1	6.0e-14	55.68	hypothetical protein SDJN03_15754, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Bhi02G001027 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7USL6	3.1e-16	31.85	GTP-binding protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3DXL1	3.4e-15	31.31	GTP-binding protein 8 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BWG6	3.4e-15	31.31	uncharacterized protein LOC103494192 OS=Cucumis melo OX=3656 GN=LOC103494192 PE=...	[more]
A0A0A0LPR1	3.6e-12	29.97	EngB-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G025170...	[more]
A0A6J1DMT7	7.4e-10	31.06	uncharacterized protein LOC111022011 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M001027	Bhi02M001027	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0046872	metal ion binding