Bhi02G001015 (gene) Wax gourd (B227) v1

Overview
NameBhi02G001015
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationchr2: 28742045 .. 28745469 (+)
RNA-Seq ExpressionBhi02G001015
SyntenyBhi02G001015
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATGGGAATGGGTATTTGCTGTTATAATTTAGAAATGTGAGATAAATGAAAATTTTACCCAAAATCTGAAAACTGAAGGGGCCGTCACTTTCATTGCATACCTTTTTCTCTTCGCTTCTCTTCTTGTTTTGCCAAAAATGTGCTGTCCAAATACTTCTCTGCCCAAAAACTGAGGAACCGAGGATTTGCACAGCCCACGAACAAGGCAAATCCGTTGAAATCAAATGGACAAAACCGATTCAATCAGTGGTTTCTCTGATCTTGTTCAACTTCCAGATTTCTCTCAGTATGTTGAAATTGATCCCACTGGCAGATATGGAAGAGTATTTCTCTACCTCTCTCTCTGACTCTCTTGCGAATTCGCCTTGATTCTGATTTTGTTCTCTTCTCTATTGCAGTACAATGAAATTCTCGGCAAAGGAGCTTCCAAGATAGTGTATGAACGAATTTAGCCTCTCCTTCGTTTCCTTTGCATTTTTGTAATTAATATCGATTTTCGGATTTCCTTGTAGTTTCACCGTTTTTTAACATGCATTTTCTATTTATTTTGAGAATTCGATCTCTTCTGCGTGTTTGATTTTTGTTCATTTCGCTCTCTTTTTCGATTTCCTCTTATGGGTTATGTTTGATTTTGTTGATTTTATTCTGTTTCTGGAAATGAGCATTCGCCTGAGATTTTCTTTTGCTTTATAGTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTTCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCACCTCTTAAAAACATTGAAACATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTAAGAATGCTTTTGAACATTCAATCTTACACCCTTCACTTTCTATTCTCTTAATTGTTGCCTTCTTTCTTTTAGATTTCTACCTCATTTCTGGTTGGTTCTGTTCTCACAGGTTTAGGCTAAAGCATAGGAGAGTTAACATTAGAGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCACTATCTCCATAGCCAAGACCCTCCTGTCATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGACCTTGGCCTTGCTGCTATCCTCCGGAAATCACATGCTGATCATTGTGTTGGTATGTTTATCTTCAAATCATGTGAAATTTTGGCAAGAGATTCAATTTTTGGACAGAAGAATCAGGCGGAGATCGTGTTACAGTTCTTTTACCATTTCATTCTTGGCTTTCTGATCCTTAATGTCCTCACGATGAATCACTTTCCTTGAGTTGCTGGTGATTTTTGTGAGAGAAATAAATCATTTTGTCAGATGTGCTTATTATTATTGTTATTACATTACAGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAGTCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGTAAGATCCCCGTCTCTCAATGGGCCTATGTTTTTCTTTAATCTAGACTATGTGAGGGTAGGAAAAGTTGATTCCTTAACATGCTTTCTACTTGTAGGGGAAAAAACCAGCTGCCTTGTATAAAGTGAAGGATCCAAGCATGCGGCAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAGTTGATGATGGTGAAAGCAAGTCGAAAATCTCAAATTCTCGAGGAGAACTGGACGATTTTGCTTCCACTATAGCTCACCCTTTCCTTGAACAAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTTGAGACCTCAGATGAATGGAGGTGCCATACAGTTCAGAAGGAAGCCGATGGGATTGAACTTTTTGAGGACGATGATAATGACATATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATCAGAGAAGATGGCAGTATCGTCTTAAGACTTAGGATTGCAGATAAAGAAGGTAGATCACATTTATTTTCCTCCTTTCGGTTTTGGTCATGGATGCTTGCTTTCTATTAATAGTTACAGAAATGTTCACCCGAACAATGCGATATATGTGCAGGACGTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTAACAGTAGCCACCGAAATGATAGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTACCTGACTGGAAGCCAGGGCCTGGCATAGATGAAACACCTCGTATCTCTTATGACGGTGGCTCTCGAAGCTACAACGATTCTAATCAACCATCTGATAATTCTTTGATAGAGAATAAGGGAAATGGAGCCAAACTCTATCAGATTTTAAACTGCAGCATAGATGGACGTGCTTTAGTGCAAGGACACTTCGAGCAAGGGCAGTTCTCCCTCAAAGCAGATCAGCCTACACAGTCTGTGGTAGAGAGCAGACCAAAAGTGTCAAGCCAACACAATCAGTCAAACTCTGCCCTCCATGAAAACCAAGCTCTTAGTTCTCATAGTTTTAGACAAAGACATTCTGGTGAAAATTACAAGAAAACTGACCAATCAGTCACTGTTGGGTATAGCAAAGAAAAAGTACCAGATACCAAAGCTACTGTCATTGATAGAACTTCACTAAGAAGTTTATTGGGTTCCCGATCGTTGTCCACGGTTCCATCCTATTGTGAAGATAAATTTGCCTCCCAAATCCATTGGGAAATTAGATGGCTATGGAATTGAGATAATTTAGAGACATCAGTTGGAACTTTTTTTGCAGTCCCTCTACATCGCTACAAGCAATAGAGGATGCAAGTGAAAACGCTGTTTTTATTGGACAAGATTGTGAAGATTTAAATCATAGTTGAGGTTATTTCGTTGTAGCTGCCCCGTTTGGATACCCAAAGGGTCCGAAACTGTGAGGCGTTAGCTAGATTTTTCTTCAATTGCAGAGTATATATTCAAGAATCAAAGATTACAGGCACATTCCCTCCAAGCAACAAAACAACTCACTACCAATTGCTGTTTTTTTATTGTACAGAAATATGTAAGCTATTCACAGCATTTACTGTACTTTTTTCACTTACATCGTCACCTTTTTTTTCTTCTTGATCATTGTTTTAGTTCACAAAGTTATTGGAATTGTAATGGATATTCTCTTATAGACAATGAAAAAAGATGCAAGTGTTCTCCTGGTT

mRNA sequence

ATTATGGGAATGGGTATTTGCTGTTATAATTTAGAAATGTGAGATAAATGAAAATTTTACCCAAAATCTGAAAACTGAAGGGGCCGTCACTTTCATTGCATACCTTTTTCTCTTCGCTTCTCTTCTTGTTTTGCCAAAAATGTGCTGTCCAAATACTTCTCTGCCCAAAAACTGAGGAACCGAGGATTTGCACAGCCCACGAACAAGGCAAATCCGTTGAAATCAAATGGACAAAACCGATTCAATCAGTGGTTTCTCTGATCTTGTTCAACTTCCAGATTTCTCTCAGTATGTTGAAATTGATCCCACTGGCAGATATGGAAGATACAATGAAATTCTCGGCAAAGGAGCTTCCAAGATAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTTCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCACCTCTTAAAAACATTGAAACATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTTTAGGCTAAAGCATAGGAGAGTTAACATTAGAGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCACTATCTCCATAGCCAAGACCCTCCTGTCATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGACCTTGGCCTTGCTGCTATCCTCCGGAAATCACATGCTGATCATTGTGTTGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAGTCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGCTGCCTTGTATAAAGTGAAGGATCCAAGCATGCGGCAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAGTTGATGATGGTGAAAGCAAGTCGAAAATCTCAAATTCTCGAGGAGAACTGGACGATTTTGCTTCCACTATAGCTCACCCTTTCCTTGAACAAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTTGAGACCTCAGATGAATGGAGGTGCCATACAGTTCAGAAGGAAGCCGATGGGATTGAACTTTTTGAGGACGATGATAATGACATATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATCAGAGAAGATGGCAGTATCGTCTTAAGACTTAGGATTGCAGATAAAGAAGGACGTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTAACAGTAGCCACCGAAATGATAGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTACCTGACTGGAAGCCAGGGCCTGGCATAGATGAAACACCTCGTATCTCTTATGACGGTGGCTCTCGAAGCTACAACGATTCTAATCAACCATCTGATAATTCTTTGATAGAGAATAAGGGAAATGGAGCCAAACTCTATCAGATTTTAAACTGCAGCATAGATGGACGTGCTTTAGTGCAAGGACACTTCGAGCAAGGGCAGTTCTCCCTCAAAGCAGATCAGCCTACACAGTCTGTGGTAGAGAGCAGACCAAAAGTGTCAAGCCAACACAATCAGTCAAACTCTGCCCTCCATGAAAACCAAGCTCTTAGTTCTCATAGTTTTAGACAAAGACATTCTGGTGAAAATTACAAGAAAACTGACCAATCAGTCACTGTTGGGTATAGCAAAGAAAAAGTACCAGATACCAAAGCTACTGTCATTGATAGAACTTCACTAAGAAGTTTATTGGGTTCCCGATCGTTGTCCACGGTTCCATCCTATTGTGAAGATAAATTTGCCTCCCAAATCCATTGGGAAATTAGATGGCTATGGAATTGAGATAATTTAGAGACATCAGTTGGAACTTTTTTTGCAGTCCCTCTACATCGCTACAAGCAATAGAGGATGCAAGTGAAAACGCTGTTTTTATTGGACAAGATTGTGAAGATTTAAATCATAGTTGAGGTTATTTCGTTGTAGCTGCCCCGTTTGGATACCCAAAGGGTCCGAAACTGTGAGGCGTTAGCTAGATTTTTCTTCAATTGCAGAGTATATATTCAAGAATCAAAGATTACAGGCACATTCCCTCCAAGCAACAAAACAACTCACTACCAATTGCTGTTTTTTTATTGTACAGAAATATGTAAGCTATTCACAGCATTTACTGTACTTTTTTCACTTACATCGTCACCTTTTTTTTCTTCTTGATCATTGTTTTAGTTCACAAAGTTATTGGAATTGTAATGGATATTCTCTTATAGACAATGAAAAAAGATGCAAGTGTTCTCCTGGTT

Coding sequence (CDS)

ATGGACAAAACCGATTCAATCAGTGGTTTCTCTGATCTTGTTCAACTTCCAGATTTCTCTCAGTATGTTGAAATTGATCCCACTGGCAGATATGGAAGATACAATGAAATTCTCGGCAAAGGAGCTTCCAAGATAGTTTACAGAGCATTTGATGAGTATGAAGGGATTGAAGTTGCTTGGAATCAGGTCAAACTCTGTAACTTTCTTCAATGTCCTGAAGATCTTGAGAGGCTATACTCTGAGATTCACCTCTTAAAAACATTGAAACATAAGAACATTATGAAATTCTATACTTCTTGGGTCGATACTGCAAATAAGAACATCAACTTTGTAACTGAAATGTTCACTTCTGGGACTTTGAGGCAGTTTAGGCTAAAGCATAGGAGAGTTAACATTAGAGCAGTGAAGCGATGGTGTAGACAAATTTTGAGGGGTCTTCACTATCTCCATAGCCAAGACCCTCCTGTCATCCACAGAGATCTCAAGTGTGACAACATTTTTGTTAATGGTAACCAAGGAGAAATTAAAATTGGAGACCTTGGCCTTGCTGCTATCCTCCGGAAATCACATGCTGATCATTGTGTTGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAATTGGTAGACATTTATTCTTTTGGGATGTGTGTTTTGGAGATGGTTACGTTTGAATATCCATATAGTGAATGTAGTCATCCTGTTCAAATCTATAAGAAAGTAATTTCTGGGAAAAAACCAGCTGCCTTGTATAAAGTGAAGGATCCAAGCATGCGGCAATTCGTTGAGAAATGCTTGGCACCAGTCTCTTGTAGACTTTCAGCAAGGGAGCTTTTGAGTGACCCTTTTCTCCAAGTTGATGATGGTGAAAGCAAGTCGAAAATCTCAAATTCTCGAGGAGAACTGGACGATTTTGCTTCCACTATAGCTCACCCTTTCCTTGAACAAGAAAAAAGATTTAGCTCAATTAGTTACAGTTTTGAGACCTCAGATGAATGGAGGTGCCATACAGTTCAGAAGGAAGCCGATGGGATTGAACTTTTTGAGGACGATGATAATGACATATTGGAAAATCTTGATAACAGTATTAAGGGGAAGATCAGAGAAGATGGCAGTATCGTCTTAAGACTTAGGATTGCAGATAAAGAAGGACGTATTCGGAACATATATTTCCCATTTGACACCAAGAATGATACGGCATTAACAGTAGCCACCGAAATGATAGCAGAACTTGATATTACTGACCAAGATGTGATCAAGATAGCTGAAAAAATTGATGGGGAAATTGCTTCCTTGGTACCTGACTGGAAGCCAGGGCCTGGCATAGATGAAACACCTCGTATCTCTTATGACGGTGGCTCTCGAAGCTACAACGATTCTAATCAACCATCTGATAATTCTTTGATAGAGAATAAGGGAAATGGAGCCAAACTCTATCAGATTTTAAACTGCAGCATAGATGGACGTGCTTTAGTGCAAGGACACTTCGAGCAAGGGCAGTTCTCCCTCAAAGCAGATCAGCCTACACAGTCTGTGGTAGAGAGCAGACCAAAAGTGTCAAGCCAACACAATCAGTCAAACTCTGCCCTCCATGAAAACCAAGCTCTTAGTTCTCATAGTTTTAGACAAAGACATTCTGGTGAAAATTACAAGAAAACTGACCAATCAGTCACTGTTGGGTATAGCAAAGAAAAAGTACCAGATACCAAAGCTACTGTCATTGATAGAACTTCACTAAGAAGTTTATTGGGTTCCCGATCGTTGTCCACGGTTCCATCCTATTGTGAAGATAAATTTGCCTCCCAAATCCATTGGGAAATTAGATGGCTATGGAATTGA

Protein sequence

MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKISNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDILENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNGAKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQALSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYCEDKFASQIHWEIRWLWN
Homology
BLAST of Bhi02G001015 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 645.6 bits (1664), Expect = 4.1e-185
Identity = 360/641 (56.16%), Postives = 437/641 (68.17%), Query Frame = 0

Query: 17  PDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S++VE+DPTGRYGRYNE+LGKGASK VYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTE+FTSGTLRQ+RL+H+RVNIRA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGLHYLHS DPPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC+HP QIYKKV+SGKKP ALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGE----------SKSKISNSRGE 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL++DDGE          S   +      
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQPHH 308

Query: 317 LDDF------ASTIAHPFLEQEKRFSSISYSFETSDEWRCH-----------TVQKEADG 376
           L D+      +S++   +       +S S + + S+ +  H              +E  G
Sbjct: 309 LPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHG 368

Query: 377 IELFEDDDNDILE------NLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKND 436
           IELFE  +ND  E      N+D +IKGK R+DG + LRLRIADKEGR+RNIYFPFD + D
Sbjct: 369 IELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETD 428

Query: 437 TALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYN 496
           TAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +    + S  
Sbjct: 429 TALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAASIC 488

Query: 497 D---SNQPSDNSLIE--NKGNGAKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVV 556
           +   SN+ S  S+++      GA    ++ C  +G     G FE+           ++ V
Sbjct: 489 NNCVSNRTSMGSVMDFLRTNPGA---NVIQCCRNGCGETHGRFEEITI-------RETEV 548

Query: 557 ESRPKVSSQHNQSNSALHENQALSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATV 616
             R     Q  Q      E++ LSS       SG N+ + ++   V Y     P+   + 
Sbjct: 549 RLRELWKLQQQQ------ESRELSSID-----SGHNHSEEEEEEEVLYED---PENMFSC 608

BLAST of Bhi02G001015 vs. TAIR 10
Match: AT3G04910.2 (with no lysine (K) kinase 1 )

HSP 1 Score: 590.1 bits (1520), Expect = 2.0e-168
Identity = 336/612 (54.90%), Postives = 409/612 (66.83%), Query Frame = 0

Query: 46  VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN 105
           +YRAFDEYEGIEVAWNQVKL +FLQ PEDLERLY EIHLLKTLKHKNIMKFYTSWVDTAN
Sbjct: 15  LYRAFDEYEGIEVAWNQVKLYDFLQSPEDLERLYCEIHLLKTLKHKNIMKFYTSWVDTAN 74

Query: 106 KNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDN 165
           +NINFVTE+FTSGTLRQ+RL+H+RVNIRA+K WCRQILRGLHYLHS DPPVIHRDLKCDN
Sbjct: 75  RNINFVTELFTSGTLRQYRLRHKRVNIRAMKHWCRQILRGLHYLHSHDPPVIHRDLKCDN 134

Query: 166 IFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLE 225
           IFVNGNQGE+KIGDLGLAAILRKSHA HCVGTPEFMAPEVYEEAYNELVDIYSFGMC+LE
Sbjct: 135 IFVNGNQGEVKIGDLGLAAILRKSHAAHCVGTPEFMAPEVYEEAYNELVDIYSFGMCILE 194

Query: 226 MVTFEYPYSECSHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 285
           MVTF+YPYSEC+HP QIYKKV+SGKKP ALYKVKDP ++ F+EKCLA VS R+SARELL 
Sbjct: 195 MVTFDYPYSECTHPAQIYKKVMSGKKPDALYKVKDPEVKCFIEKCLATVSLRVSARELLD 254

Query: 286 DPFLQVDDGE----------SKSKISNSRGELDDF------ASTIAHPFLEQEKRFSSIS 345
           DPFL++DDGE          S   +      L D+      +S++   +       +S S
Sbjct: 255 DPFLRIDDGEFDLRSVDMEDSVGPLYRQPHHLPDYYNYPSNSSSLNRQYSNGNYPSNSSS 314

Query: 346 YSFETSDEWRCH-----------TVQKEADGIELFEDDDNDILE------NLDNSIKGKI 405
            + + S+ +  H              +E  GIELFE  +ND  E      N+D +IKGK 
Sbjct: 315 LNRQYSNGYNSHHEYQNGWAYNPAETEETHGIELFESRNNDDQEEEKKSGNVDITIKGKR 374

Query: 406 REDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDG 465
           R+DG + LRLRIADKEGR+RNIYFPFD + DTAL+VATEM+AELD+ D  V KIA  IDG
Sbjct: 375 RDDGGLFLRLRIADKEGRVRNIYFPFDIETDTALSVATEMVAELDMDDHGVTKIANMIDG 434

Query: 466 EIASLVPDWKPGPGIDETPRISYDGGSRSYND---SNQPSDNSLIE--NKGNGAKLYQIL 525
           EI+SLVP W+PGP  +E    +    + S  +   SN+ S  S+++      GA    ++
Sbjct: 435 EISSLVPSWRPGPEFEECLAAAAAANAASICNNCVSNRTSMGSVMDFLRTNPGA---NVI 494

Query: 526 NCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQALSSHSFR 585
            C  +G     G FE+           ++ V  R     Q  Q      E++ LSS    
Sbjct: 495 QCCRNGCGETHGRFEEITI-------RETEVRLRELWKLQQQQ------ESRELSSID-- 554

Query: 586 QRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPS-YCE---DK 616
              SG N+ + ++   V Y     P+   +      +  + GS S S +PS YC+   +K
Sbjct: 555 ---SGHNHSEEEEEEEVLYED---PENMFSCEAGNEINHISGSGSFSFMPSKYCDEPSEK 602

BLAST of Bhi02G001015 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.6e-165
Identity = 299/500 (59.80%), Postives = 371/500 (74.20%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S+YVE+DPTGRYGRYNE+LGKG+SK VYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT N+NINFVTEMFTSGTLRQ+RLKH+RVNIRAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL+YLH+ DPPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSECSHP QIYK+VISGKKP  L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKISNSRGELDDFASTIAHP 317
           VKDP +R F+EKCLA VS RLSA ELL D FL +D+ + + ++ + +G +D+  + + H 
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESDMR-RVESEKGLIDEAGTPLRHS 310

Query: 318 FLEQEKRFSSISYSFETSDEWRCH---TVQK-EADGIELFEDDDNDILE----NLDNSIK 377
           +      +S+  YS    ++W  +   TV+  E D +E   DDD +  +    ++D SIK
Sbjct: 311 Y--HIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIK 370

Query: 378 GKIREDG-SIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           GK R++G  + LRL+  +KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV KIA 
Sbjct: 371 GKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVTKIAN 430

Query: 438 KIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNGAKLYQILN 497
            IDGEIASLVP+W                     ++SN+ S  S+++        +  + 
Sbjct: 431 MIDGEIASLVPNW----------------SIFCSSESNRSSVGSVMD--------FNEMQ 483

Query: 498 CSIDGRALVQGHFEQGQFSL 509
           C  DG     G FE+  F +
Sbjct: 491 CGRDGCEEKHGRFEEITFEI 483

BLAST of Bhi02G001015 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 574.3 bits (1479), Expect = 1.2e-163
Identity = 293/427 (68.62%), Postives = 338/427 (79.16%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRY+EILGKGASK VYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC+HP QIYKKV SGKKP A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVD--DGESKSKISNSRGELDDFASTIA 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D  DG     I    G  D+    + 
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNG-YDETGVFLR 309

Query: 318 HPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDILENLDNSIKGKIRED 377
           HP ++           FE+S          +   I+LF +DD D   ++D SIKGK   D
Sbjct: 310 HPLIDDPLYHD----QFESS----------QICEIDLFANDDED---HVDISIKGKRNGD 369

Query: 378 GSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIA 437
             I LRLRI+D EGRIRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA
Sbjct: 370 DGIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIA 418

Query: 438 SLVPDWK 443
           +LVPDWK
Sbjct: 430 ALVPDWK 418

BLAST of Bhi02G001015 vs. TAIR 10
Match: AT3G22420.2 (with no lysine (K) kinase 2 )

HSP 1 Score: 543.1 bits (1398), Expect = 2.9e-154
Identity = 293/486 (60.29%), Postives = 338/486 (69.55%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRY+EILGKGASK VYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCV-- 197
           WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CV  
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 ------------------------------GTPEFMAPEVYEEAYNELVDIYSFGMCVLE 257
                                         GTPEFMAPEVY+E YNELVD+Y+FGMCVLE
Sbjct: 190 SKPSHHWNFIALIMFFTTLDLPLLCLCVVKGTPEFMAPEVYDEEYNELVDVYAFGMCVLE 249

Query: 258 MVTFEYPYSECSHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLS 317
           MVTF+YPYSEC+HP QIYKKV SGKKP A Y VKDP +R+FVEKCLA V+CRL+A ELL 
Sbjct: 250 MVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQ 309

Query: 318 DPFLQVD--DGESKSKISNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTV 377
           DPFLQ D  DG     I    G  D+    + HP ++           FE+S        
Sbjct: 310 DPFLQDDNMDGFVMRPIDYYNG-YDETGVFLRHPLIDDPLYHD----QFESS-------- 369

Query: 378 QKEADGIELFEDDDNDILENLDNSIKGKIREDGSIVLRLRIADKE--------------- 437
             +   I+LF +DD D   ++D SIKGK   D  I LRLRI+D E               
Sbjct: 370 --QICEIDLFANDDED---HVDISIKGKRNGDDGIFLRLRISDAEGIVSIFFDSFKIIGL 429

Query: 438 ------------GRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIAS 443
                       GRIRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA+
Sbjct: 430 KNVTRFRVLGNVGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAA 477

BLAST of Bhi02G001015 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.8e-184
Identity = 360/641 (56.16%), Postives = 437/641 (68.17%), Query Frame = 0

Query: 17  PDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLE 76
           PD+S++VE+DPTGRYGRYNE+LGKGASK VYRAFDEYEGIEVAWNQVKL +FLQ PEDLE
Sbjct: 9   PDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLE 68

Query: 77  RLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVK 136
           RLY EIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTE+FTSGTLRQ+RL+H+RVNIRA+K
Sbjct: 69  RLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMK 128

Query: 137 RWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVG 196
            WCRQILRGLHYLHS DPPVIHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVG
Sbjct: 129 HWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCVG 188

Query: 197 TPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALY 256
           TPEFMAPEVYEEAYNELVDIYSFGMC+LEMVTF+YPYSEC+HP QIYKKV+SGKKP ALY
Sbjct: 189 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 248

Query: 257 KVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGE----------SKSKISNSRGE 316
           KVKDP ++ F+EKCLA VS R+SARELL DPFL++DDGE          S   +      
Sbjct: 249 KVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFDLRSVDMEDSVGPLYRQPHH 308

Query: 317 LDDF------ASTIAHPFLEQEKRFSSISYSFETSDEWRCH-----------TVQKEADG 376
           L D+      +S++   +       +S S + + S+ +  H              +E  G
Sbjct: 309 LPDYYNYPSNSSSLNRQYSNGNYPSNSSSLNRQYSNGYNSHHEYQNGWAYNPAETEETHG 368

Query: 377 IELFEDDDNDILE------NLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKND 436
           IELFE  +ND  E      N+D +IKGK R+DG + LRLRIADKEGR+RNIYFPFD + D
Sbjct: 369 IELFESRNNDDQEEEKKSGNVDITIKGKRRDDGGLFLRLRIADKEGRVRNIYFPFDIETD 428

Query: 437 TALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYN 496
           TAL+VATEM+AELD+ D  V KIA  IDGEI+SLVP W+PGP  +E    +    + S  
Sbjct: 429 TALSVATEMVAELDMDDHGVTKIANMIDGEISSLVPSWRPGPEFEECLAAAAAANAASIC 488

Query: 497 D---SNQPSDNSLIE--NKGNGAKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVV 556
           +   SN+ S  S+++      GA    ++ C  +G     G FE+           ++ V
Sbjct: 489 NNCVSNRTSMGSVMDFLRTNPGA---NVIQCCRNGCGETHGRFEEITI-------RETEV 548

Query: 557 ESRPKVSSQHNQSNSALHENQALSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATV 616
             R     Q  Q      E++ LSS       SG N+ + ++   V Y     P+   + 
Sbjct: 549 RLRELWKLQQQQ------ESRELSSID-----SGHNHSEEEEEEEVLYED---PENMFSC 608

BLAST of Bhi02G001015 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 629.8 bits (1623), Expect = 3.3e-179
Identity = 332/534 (62.17%), Postives = 387/534 (72.47%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D  +Y E+DPTGRYGRYN++LGKGASK VYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + +NINF+TEMFTSGTLRQ+R KH RVNI AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GL YLHS DPP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC+HPVQIYKKVISG KP ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDD-----GESKSKISNSRGELDDFAS 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQVDD     G+    + N   +      
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDYSLMNYLRQ-----P 312

Query: 318 TIAHPFLEQEKRFSSISYSFE----TSDEWRCHTVQKEADGIELFEDDDNDILENLDNSI 377
            + H +       + +S S +    T D W C     +ADGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIAD +G +RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYN-DSNQPSDNSLIENKGNGAKLYQIL 497
            IDGE+++LVPDW+PGPGI+E+   +Y      +N  SN  S  SL     + A+  Q  
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTY-----CHNCGSNVSSCGSLYAYMSSAARGCQC- 492

Query: 498 NCSIDGRALVQGHFEQGQFSLKADQ---PTQSVVESRPKVSSQHNQSNSALHEN 539
                  A + G FE+  F    +Q                +QH +   A+H N
Sbjct: 493 -------AELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSN 528

BLAST of Bhi02G001015 vs. ExPASy Swiss-Prot
Match: Q0D598 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK1 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.3e-179
Identity = 332/534 (62.17%), Postives = 387/534 (72.47%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D  +Y E+DPTGRYGRYN++LGKGASK VYRAFDEY+G+EVAWNQVKL +FLQ PEDLER
Sbjct: 13  DLPEYAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLER 72

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
           LY EIHLLKTLKH+NIMKFYTSWVD + +NINF+TEMFTSGTLRQ+R KH RVNI AVK 
Sbjct: 73  LYCEIHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKH 132

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQIL GL YLHS DPP+IHRDLKCDNIFVNGNQGE+KIGDLGLAAILRKSHA HCVGT
Sbjct: 133 WCRQILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAVHCVGT 192

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVYEE YNELVDIYSFGMCVLEMVTFEYPYSEC+HPVQIYKKVISG KP ALYK
Sbjct: 193 PEFMAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYK 252

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDD-----GESKSKISNSRGELDDFAS 317
           VKDP +RQFVEKCLA  S RLSARELL DPFLQVDD     G+    + N   +      
Sbjct: 253 VKDPMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDYSLMNYLRQ-----P 312

Query: 318 TIAHPFLEQEKRFSSISYSFE----TSDEWRCHTVQKEADGIELFEDDDNDILENLDNSI 377
            + H +       + +S S +    T D W C     +ADGI+LF   +++ L N+D +I
Sbjct: 313 YLEHAYSNVSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITI 372

Query: 378 KGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           KG+  EDGSI LRLRIAD +G +RNIYFPFD + DTAL+VATEM+AELDITD +V +IAE
Sbjct: 373 KGRKSEDGSIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAE 432

Query: 438 KIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYN-DSNQPSDNSLIENKGNGAKLYQIL 497
            IDGE+++LVPDW+PGPGI+E+   +Y      +N  SN  S  SL     + A+  Q  
Sbjct: 433 MIDGEVSALVPDWRPGPGIEESQDTTY-----CHNCGSNVSSCGSLYAYMSSAARGCQC- 492

Query: 498 NCSIDGRALVQGHFEQGQFSLKADQ---PTQSVVESRPKVSSQHNQSNSALHEN 539
                  A + G FE+  F    +Q                +QH +   A+H N
Sbjct: 493 -------AELHGRFEEITFQANGEQTDLQDSGGSSDDGGGQTQHVKDQEAVHSN 528

BLAST of Bhi02G001015 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 2.3e-164
Identity = 299/500 (59.80%), Postives = 371/500 (74.20%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D+S+YVE+DPTGRYGRYNE+LGKG+SK VYR FDEY+GIEVAWNQVKL +FLQ P++LER
Sbjct: 11  DYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQELER 70

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
           LY EIHLLKTLKHK+IMKFY SWVDT N+NINFVTEMFTSGTLRQ+RLKH+RVNIRAVK 
Sbjct: 71  LYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAVKN 130

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WCRQILRGL+YLH+ DPPVIHRDLKCDNIF+NGNQGE+KIGDLGLAA L+ SHA HCVGT
Sbjct: 131 WCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCVGT 190

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVY+E YN+LVDIYSFGMCVLEMVTF+YPYSECSHP QIYK+VISGKKP  L K
Sbjct: 191 PEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDGLDK 250

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKISNSRGELDDFASTIAHP 317
           VKDP +R F+EKCLA VS RLSA ELL D FL +D+ + + ++ + +G +D+  + + H 
Sbjct: 251 VKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESDMR-RVESEKGLIDEAGTPLRHS 310

Query: 318 FLEQEKRFSSISYSFETSDEWRCH---TVQK-EADGIELFEDDDNDILE----NLDNSIK 377
           +      +S+  YS    ++W  +   TV+  E D +E   DDD +  +    ++D SIK
Sbjct: 311 Y--HIPHYSNGYYSLYNQNQWDYNGDETVESHEIDLLEFQNDDDEEEEDKRFGSVDISIK 370

Query: 378 GKIREDG-SIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAE 437
           GK R++G  + LRL+  +KEG +RNIYFPFD + DTA++VA EM+ EL++ D+DV KIA 
Sbjct: 371 GKRRDNGDGLFLRLKTVNKEGCVRNIYFPFDIETDTAISVAREMVEELEMDDRDVTKIAN 430

Query: 438 KIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNGAKLYQILN 497
            IDGEIASLVP+W                     ++SN+ S  S+++        +  + 
Sbjct: 431 MIDGEIASLVPNW----------------SIFCSSESNRSSVGSVMD--------FNEMQ 483

Query: 498 CSIDGRALVQGHFEQGQFSL 509
           C  DG     G FE+  F +
Sbjct: 491 CGRDGCEEKHGRFEEITFEI 483

BLAST of Bhi02G001015 vs. ExPASy Swiss-Prot
Match: Q8S8Y9 (Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=1 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.6e-162
Identity = 293/427 (68.62%), Postives = 338/427 (79.16%), Query Frame = 0

Query: 18  DFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLER 77
           D S +VEIDP+GRYGRY+EILGKGASK VYRAFDEYEGIEVAWNQVKL NF + PE+LE+
Sbjct: 10  DCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEK 69

Query: 78  LYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKR 137
            + EIHLLKTL H+NIMKFYTSWVDT N +INFVTE+FTSGTLRQ+RL+HRRVNIRAVK+
Sbjct: 70  FFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQ 129

Query: 138 WCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGT 197
           WC+QIL+GL YLHS+ PP+IHRDLKCDNIF+NGNQGE+KIGDLGLAAILRKSHA  CVGT
Sbjct: 130 WCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVRCVGT 189

Query: 198 PEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYK 257
           PEFMAPEVY+E YNELVD+Y+FGMCVLEMVTF+YPYSEC+HP QIYKKV SGKKP A Y 
Sbjct: 190 PEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYL 249

Query: 258 VKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVD--DGESKSKISNSRGELDDFASTIA 317
           VKDP +R+FVEKCLA V+CRL+A ELL DPFLQ D  DG     I    G  D+    + 
Sbjct: 250 VKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNG-YDETGVFLR 309

Query: 318 HPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDILENLDNSIKGKIRED 377
           HP ++           FE+S          +   I+LF +DD D   ++D SIKGK   D
Sbjct: 310 HPLIDDPLYHD----QFESS----------QICEIDLFANDDED---HVDISIKGKRNGD 369

Query: 378 GSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIA 437
             I LRLRI+D EGRIRNIYFPF+T  DTA +VA EM++ELDIT+QDV KIAE ID EIA
Sbjct: 370 DGIFLRLRISDAEGRIRNIYFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIA 418

Query: 438 SLVPDWK 443
           +LVPDWK
Sbjct: 430 ALVPDWK 418

BLAST of Bhi02G001015 vs. NCBI nr
Match: XP_038880702.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 617/617 (100.00%), Postives = 617/617 (100.00%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI
Sbjct: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG
Sbjct: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
           AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA
Sbjct: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC
Sbjct: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 617

BLAST of Bhi02G001015 vs. NCBI nr
Match: XP_038880703.1 (probable serine/threonine-protein kinase WNK9 isoform X2 [Benincasa hispida])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 582/617 (94.33%), Postives = 582/617 (94.33%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW
Sbjct: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL                           
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNEL--------------------------- 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
                   GKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI
Sbjct: 241 --------GKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI
Sbjct: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG
Sbjct: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
           AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA
Sbjct: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC
Sbjct: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 582

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 582

BLAST of Bhi02G001015 vs. NCBI nr
Match: XP_008462723.1 (PREDICTED: probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis melo])

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 545/617 (88.33%), Postives = 566/617 (91.73%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAIL+KSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL++D  ESK KI
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S+SR ELDDFASTI  PFLE+EKRFSSISYS E SDEWR  +VQKE DGIELFED+DND 
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLD +IKGKIREDGSIVLRLRI DKEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEI+SLVP+WKPGPGIDETPRISYDGGS+SYN SNQPSDN LIENKGNG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S DG AL   HFEQ QFSLKAD+PTQ VV S P VSSQH Q +S L ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSS SFRQRH  +NYKKTDQS+ VGY KEK+PD KATVID TS RSLLGSRSLS V SYC
Sbjct: 541 LSSRSFRQRHYDDNYKKTDQSLAVGY-KEKLPDKKATVID-TSQRSLLGSRSLSRVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 615

BLAST of Bhi02G001015 vs. NCBI nr
Match: XP_004142568.1 (probable serine/threonine-protein kinase WNK9 isoform X2 [Cucumis sativus] >KGN66804.1 hypothetical protein Csa_006948 [Cucumis sativus])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 543/617 (88.01%), Postives = 567/617 (91.90%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGRY+EILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYDEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL++D  ESK KI
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDGCESKLKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S+SR ELDDFASTI  PFLE+EKRFSSISYS E SDEWR  +VQKE DGIELFED+DND 
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEGSDEWRYRSVQKEPDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           L +LDN+IKGKIREDGSIVLRLRI DKEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LVSLDNNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEI+SLVP+WKPGPGIDETPRISYDGGS+SYN  NQPSDN LIENKGNG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNACNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S DG AL   HFEQ QFSLKAD+PTQ      P VSSQH Q +S L+ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHEHFEQEQFSLKADRPTQ------PNVSSQHYQPDSVLNENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSSHSFRQRHS +NYKK DQS+TVGY+KEK+P  KATVID TS RSLLGSRSLSTV SYC
Sbjct: 541 LSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPVNKATVID-TSQRSLLGSRSLSTVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 610

BLAST of Bhi02G001015 vs. NCBI nr
Match: XP_031736005.1 (probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis sativus])

HSP 1 Score: 1064.3 bits (2751), Expect = 4.0e-307
Identity = 543/645 (84.19%), Postives = 567/645 (87.91%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGR--------------------------- 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGR                           
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRVLFSLLFLSHYLPNSPSFLFSSFLFCL 60

Query: 61  -YNEILGKGASKIVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120
            Y+EILGKGASK VYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN
Sbjct: 61  QYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLCNFLQCPEDLERLYSEIHLLKTLKHKN 120

Query: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQ 180
           IMKFYTSWVDTANKNINFVTEMFTSGTLRQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQ
Sbjct: 121 IMKFYTSWVDTANKNINFVTEMFTSGTLRQYRLKHRRANIRAVKRWCRQILRGLHYLHSQ 180

Query: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNE 240
           DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNE
Sbjct: 181 DPPVIHRDLKCDNIFVNGNQGEIKIGDLGLAAILRKSHADHCVGTPEFMAPEVYEEAYNE 240

Query: 241 LVDIYSFGMCVLEMVTFEYPYSECSHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLA 300
           LVDIYSFGMCVLEMVTFEYPYSEC+HPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLA
Sbjct: 241 LVDIYSFGMCVLEMVTFEYPYSECNHPVQIYKKVISGKKPAALYKVKDPSMRQFVEKCLA 300

Query: 301 PVSCRLSARELLSDPFLQVDDGESKSKISNSRGELDDFASTIAHPFLEQEKRFSSISYSF 360
           PVSCRLSARELLSDPFL++D  ESK KIS+SR ELDDFASTI  PFLE+EKRFSSISYS 
Sbjct: 301 PVSCRLSARELLSDPFLEIDGCESKLKISDSRRELDDFASTIVRPFLEREKRFSSISYSL 360

Query: 361 ETSDEWRCHTVQKEADGIELFEDDDNDILENLDNSIKGKIREDGSIVLRLRIADKEGRIR 420
           E SDEWR  +VQKE DGIELFED+DND L +LDN+IKGKIREDGSIVLRLRI DKEG IR
Sbjct: 361 EGSDEWRYRSVQKEPDGIELFEDNDNDQLVSLDNNIKGKIREDGSIVLRLRITDKEGLIR 420

Query: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEIASLVPDWKPGPGIDETPR 480
           NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEI+SLVP+WKPGPGIDETPR
Sbjct: 421 NIYFPFDTKNDTALTVATEMIAELDITDQDVIKIAEKIDGEISSLVPEWKPGPGIDETPR 480

Query: 481 ISYDGGSRSYNDSNQPSDNSLIENKGNGAKLYQILNCSIDGRALVQGHFEQGQFSLKADQ 540
           ISYDGGS+SYN  NQPSDN LIENKGNG KLYQILN S DG AL   HFEQ QFSLKAD+
Sbjct: 481 ISYDGGSQSYNACNQPSDNILIENKGNGIKLYQILNLSTDGHALAHEHFEQEQFSLKADR 540

Query: 541 PTQSVVESRPKVSSQHNQSNSALHENQALSSHSFRQRHSGENYKKTDQSVTVGYSKEKVP 600
           PTQ      P VSSQH Q +S L+ENQALSSHSFRQRHS +NYKK DQS+TVGY+KEK+P
Sbjct: 541 PTQ------PNVSSQHYQPDSVLNENQALSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLP 600

Query: 601 DTKATVIDRTSLRSLLGSRSLSTVPSYCEDKFASQIHWEIRWLWN 618
             KATVID TS RSLLGSRSLSTV SYCEDKF+SQIHWEIRWLWN
Sbjct: 601 VNKATVID-TSQRSLLGSRSLSTVSSYCEDKFSSQIHWEIRWLWN 638

BLAST of Bhi02G001015 vs. ExPASy TrEMBL
Match: A0A1S3CI45 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103501021 PE=4 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 545/617 (88.33%), Postives = 566/617 (91.73%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAIL+KSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILQKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL++D  ESK KI
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDICESKLKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S+SR ELDDFASTI  PFLE+EKRFSSISYS E SDEWR  +VQKE DGIELFED+DND 
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEASDEWRYRSVQKERDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLD +IKGKIREDGSIVLRLRI DKEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDKNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEI+SLVP+WKPGPGIDETPRISYDGGS+SYN SNQPSDN LIENKGNG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNASNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S DG AL   HFEQ QFSLKAD+PTQ VV S P VSSQH Q +S L ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHKHFEQEQFSLKADRPTQPVVGSIPNVSSQHYQPDSVLPENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSS SFRQRH  +NYKKTDQS+ VGY KEK+PD KATVID TS RSLLGSRSLS V SYC
Sbjct: 541 LSSRSFRQRHYDDNYKKTDQSLAVGY-KEKLPDKKATVID-TSQRSLLGSRSLSRVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 615

BLAST of Bhi02G001015 vs. ExPASy TrEMBL
Match: A0A0A0M0J2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G695390 PE=4 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 543/617 (88.01%), Postives = 567/617 (91.90%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           MD TDSI+ FS L+Q PDFS YVEIDPTGRYGRY+EILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MDTTDSITAFSHLLQPPDFSHYVEIDPTGRYGRYDEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRR NIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRANIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFL++D  ESK KI
Sbjct: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLEIDGCESKLKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S+SR ELDDFASTI  PFLE+EKRFSSISYS E SDEWR  +VQKE DGIELFED+DND 
Sbjct: 301 SDSRRELDDFASTIVRPFLEREKRFSSISYSLEGSDEWRYRSVQKEPDGIELFEDNDNDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           L +LDN+IKGKIREDGSIVLRLRI DKEG IRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LVSLDNNIKGKIREDGSIVLRLRITDKEGLIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEI+SLVP+WKPGPGIDETPRISYDGGS+SYN  NQPSDN LIENKGNG
Sbjct: 421 QDVIKIAEKIDGEISSLVPEWKPGPGIDETPRISYDGGSQSYNACNQPSDNILIENKGNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S DG AL   HFEQ QFSLKAD+PTQ      P VSSQH Q +S L+ENQA
Sbjct: 481 IKLYQILNLSTDGHALAHEHFEQEQFSLKADRPTQ------PNVSSQHYQPDSVLNENQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSSHSFRQRHS +NYKK DQS+TVGY+KEK+P  KATVID TS RSLLGSRSLSTV SYC
Sbjct: 541 LSSHSFRQRHSDDNYKKIDQSLTVGYNKEKLPVNKATVID-TSQRSLLGSRSLSTVSSYC 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKF+SQIHWEIRWLWN
Sbjct: 601 EDKFSSQIHWEIRWLWN 610

BLAST of Bhi02G001015 vs. ExPASy TrEMBL
Match: A0A6J1F099 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441166 PE=4 SV=1)

HSP 1 Score: 1000.7 bits (2586), Expect = 2.6e-288
Identity = 511/617 (82.82%), Postives = 543/617 (88.01%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           M+  DSI+GFS+L+Q PDFSQYVEIDPTGRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKV+SGKKP ALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQ+DD ESK ++
Sbjct: 241 QIYKKVVSGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S    ELD  A T AHPFLE++  FSSI+Y  E SDEWRCH+VQ E DG ELFE+DD+D 
Sbjct: 301 SGCPRELDGIAFTTAHPFLERQGSFSSINYRLEASDEWRCHSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEIASLVP+WK GPGIDETPRISYDG  +S++ +N   D+SLI N  NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGDCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S   RA V   F+Q   SLKAD+P + VVES PKVSSQH Q NS L E+ A
Sbjct: 481 EKLYQILNLSTVERASVHELFKQD--SLKADRPPEPVVESPPKVSSQHYQPNSVLLEDPA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           L SHSFR  HS +N+KK  QS+ VG  KEK+ D  A  I RTSLRSLLGSRSLSTVPSY 
Sbjct: 541 LGSHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

BLAST of Bhi02G001015 vs. ExPASy TrEMBL
Match: A0A6J1DKV6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020998 PE=4 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 9.9e-288
Identity = 511/617 (82.82%), Postives = 540/617 (87.52%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           M+K +SI GFS+L   PD S+YVEIDP+GRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   MEKDESIDGFSNL-HHPDSSRYVEIDPSGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCN LQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTAN+NINFVTEMFTSG+L
Sbjct: 61  NQVKLCNLLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTEMFTSGSL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQFRLKHRRVN RAVKRWCRQILR L YLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL
Sbjct: 121 RQFRLKHRRVNNRAVKRWCRQILRALLYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHAD CVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADRCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKV SG+KPAALYKVKDPSMRQFVEKCLAPV  RLSARELLSDPFLQ+DD ESKSKI
Sbjct: 241 QIYKKVTSGEKPAALYKVKDPSMRQFVEKCLAPVPYRLSARELLSDPFLQIDDCESKSKI 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S+ +GELD  ASTI  PFLE+E  FSSI+YS +TSDEWRC  V+ E DG ELFEDDD+D 
Sbjct: 301 SDCQGELDRIASTIVQPFLEREISFSSINYSLDTSDEWRCRIVETEPDGTELFEDDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LEN+DNSI GKIREDGSIVLRLRIADKEGRIRNIYFPFDTK DTALTVATEMIAELDITD
Sbjct: 361 LENVDNSITGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKMDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDV KIAEKIDGEIASLVP+WKPGPGIDETPRISYDG  +SY+ SN PS N LIE   NG
Sbjct: 421 QDVTKIAEKIDGEIASLVPEWKPGPGIDETPRISYDGDLQSYDTSNHPSYNHLIEKMQNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
           AK  QILNCS D RA    HFE  Q S K DQPTQ VVE  PKVS+QH Q NS LHE QA
Sbjct: 481 AKNSQILNCSADERASAPRHFE--QVSHKEDQPTQPVVERIPKVSNQHYQLNSVLHEGQA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           LSSHSFRQ HS +N KKT Q +T G   EK+  +KAT I ++S+RSLLGSRSLS VPSY 
Sbjct: 541 LSSHSFRQSHSDQNNKKTGQLITGGNDIEKIQVSKATAIAKSSIRSLLGSRSLSRVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKFASQIHWEIRWLWN
Sbjct: 601 EDKFASQIHWEIRWLWN 614

BLAST of Bhi02G001015 vs. ExPASy TrEMBL
Match: A0A6J1KJY6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495945 PE=4 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.3e-287
Identity = 511/617 (82.82%), Postives = 542/617 (87.84%), Query Frame = 0

Query: 1   MDKTDSISGFSDLVQLPDFSQYVEIDPTGRYGRYNEILGKGASKIVYRAFDEYEGIEVAW 60
           M+  DSI+GFS+L+Q PDFSQYVEIDPTGRYGRYNEILGKGASK VYRAFDEYEGIEVAW
Sbjct: 1   METIDSITGFSNLLQPPDFSQYVEIDPTGRYGRYNEILGKGASKTVYRAFDEYEGIEVAW 60

Query: 61  NQVKLCNFLQCPEDLERLYSEIHLLKTLKHKNIMKFYTSWVDTANKNINFVTEMFTSGTL 120
           NQVKLCNFLQCPEDLERLYSEIHLLK+LKHKNIMKFYTSWVDTAN+NINFVTEMFTSGTL
Sbjct: 61  NQVKLCNFLQCPEDLERLYSEIHLLKSLKHKNIMKFYTSWVDTANRNINFVTEMFTSGTL 120

Query: 121 RQFRLKHRRVNIRAVKRWCRQILRGLHYLHSQDPPVIHRDLKCDNIFVNGNQGEIKIGDL 180
           RQ+RLKHRRVNIRAVKRWCRQILRGL YLHSQ+PPVIHRDLKCDNI VNGNQGEIKIGDL
Sbjct: 121 RQYRLKHRRVNIRAVKRWCRQILRGLLYLHSQNPPVIHRDLKCDNILVNGNQGEIKIGDL 180

Query: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECSHPV 240
           GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSEC+HPV
Sbjct: 181 GLAAILRKSHADHCVGTPEFMAPEVYEEAYNELVDIYSFGMCVLEMVTFEYPYSECNHPV 240

Query: 241 QIYKKVISGKKPAALYKVKDPSMRQFVEKCLAPVSCRLSARELLSDPFLQVDDGESKSKI 300
           QIYKKVISGKKP ALYKVKDPSMRQFVEKCLAPVSCR SARELLSDPFLQ+DD ESK ++
Sbjct: 241 QIYKKVISGKKPDALYKVKDPSMRQFVEKCLAPVSCRPSARELLSDPFLQIDDCESKLQL 300

Query: 301 SNSRGELDDFASTIAHPFLEQEKRFSSISYSFETSDEWRCHTVQKEADGIELFEDDDNDI 360
           S    EL+  A T AHPFLE++  FSSI+Y  E SDEWRC +VQ E DG ELFE+DD+D 
Sbjct: 301 SGCPRELNGIAFTTAHPFLERQGSFSSINYCLEASDEWRCRSVQTEPDGFELFENDDDDQ 360

Query: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420
           LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD
Sbjct: 361 LENLDNSIKGKIREDGSIVLRLRIADKEGRIRNIYFPFDTKNDTALTVATEMIAELDITD 420

Query: 421 QDVIKIAEKIDGEIASLVPDWKPGPGIDETPRISYDGGSRSYNDSNQPSDNSLIENKGNG 480
           QDVIKIAEKIDGEIASLVP+WK GPGIDETPRISYDGG +S++ +N   D+SLI N  NG
Sbjct: 421 QDVIKIAEKIDGEIASLVPEWKSGPGIDETPRISYDGGCQSHDAANHSCDHSLIGNNQNG 480

Query: 481 AKLYQILNCSIDGRALVQGHFEQGQFSLKADQPTQSVVESRPKVSSQHNQSNSALHENQA 540
            KLYQILN S   RA V   F+Q   SLKAD P + VVES PKVSSQH Q NS L EN A
Sbjct: 481 EKLYQILNLSTVERASVHELFKQD--SLKADPPPEPVVESPPKVSSQHYQPNSVLLENPA 540

Query: 541 LSSHSFRQRHSGENYKKTDQSVTVGYSKEKVPDTKATVIDRTSLRSLLGSRSLSTVPSYC 600
           L SHSFR  HS +N+KK  QS+ VG  KEK+ D  A  I RTSLRSLLGSRSLSTVPSY 
Sbjct: 541 LGSHSFRHSHSNQNFKKIGQSIIVGDDKEKILDKNA--IARTSLRSLLGSRSLSTVPSYR 600

Query: 601 EDKFASQIHWEIRWLWN 618
           EDKFASQIH EIRWLWN
Sbjct: 601 EDKFASQIHREIRWLWN 613

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G04910.14.1e-18556.16with no lysine (K) kinase 1 [more]
AT3G04910.22.0e-16854.90with no lysine (K) kinase 1 [more]
AT5G28080.21.6e-16559.80Protein kinase superfamily protein [more]
AT3G22420.11.2e-16368.62with no lysine (K) kinase 2 [more]
AT3G22420.22.9e-15460.29with no lysine (K) kinase 2 [more]
Match NameE-valueIdentityDescription
Q9CAV65.8e-18456.16Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
A2YMV63.3e-17962.17Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=3... [more]
Q0D5983.3e-17962.17Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. japonica OX... [more]
Q2V3382.3e-16459.80Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y91.6e-16268.62Serine/threonine-protein kinase WNK2 OS=Arabidopsis thaliana OX=3702 GN=WNK2 PE=... [more]
Match NameE-valueIdentityDescription
XP_038880702.10.0e+00100.00probable serine/threonine-protein kinase WNK9 isoform X1 [Benincasa hispida][more]
XP_038880703.10.0e+0094.33probable serine/threonine-protein kinase WNK9 isoform X2 [Benincasa hispida][more]
XP_008462723.10.0e+0088.33PREDICTED: probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis mel... [more]
XP_004142568.10.0e+0088.01probable serine/threonine-protein kinase WNK9 isoform X2 [Cucumis sativus] >KGN6... [more]
XP_031736005.14.0e-30784.19probable serine/threonine-protein kinase WNK9 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A1S3CI450.0e+0088.33Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A0A0M0J20.0e+0088.01Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1... [more]
A0A6J1F0992.6e-28882.82Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DKV69.9e-28882.82Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1KJY61.3e-28782.82Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 32..289
e-value: 2.4E-59
score: 213.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 35..289
e-value: 7.3E-49
score: 166.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 32..289
score: 38.44944
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 378..433
e-value: 1.7E-5
score: 24.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 18..113
e-value: 2.6E-32
score: 112.8
NoneNo IPR availableGENE3D3.10.20.90coord: 376..441
e-value: 1.6E-5
score: 27.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 115..295
e-value: 1.9E-49
score: 169.6
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 37..296
e-value: 8.6E-11
score: 39.1
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 38..249
e-value: 4.0E-20
score: 69.5
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 38..249
e-value: 4.0E-20
score: 69.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..567
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 454..476
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..548
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 456..476
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 16..617
NoneNo IPR availablePANTHERPTHR13902:SF144SERINE/THREONINE-PROTEIN KINASE WNK1coord: 16..617
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 30..289
e-value: 1.48441E-171
score: 485.963
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 156..168
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 35..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001015Bhi02M001015mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity