Bhi02G000959 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000959
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionprotein NRT1/ PTR FAMILY 1.2-like
Locationchr2: 26340977 .. 26342999 (-)
RNA-Seq ExpressionBhi02G000959
SyntenyBhi02G000959
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGCAGAATTCAGAGGAAGTAATGGAAACCACTCAAAAGGTTACCAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTCATTTTAGGTAATATTCTTCACCAAGTTTTAAAAGTTCTGATGCATCCATTTATAATGTGTTCATTGACAAAAGAACTCTAAACTCTGCAGCAAACGAGATATTTGAGAAGATTTCAAATATTGGTCTTCATGCAAATATGATATTTTACCTGATAAATGAGTATCACCTGAGTAGTGCTAAAGGAGCAATCGTCTTGTTTCTGTGGTCTGCACTGACAAACTTTTTGCCCATTGCCGGAGCTTTCCTCTCTGATTCTTACTTGGGTCGGTTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTCTTGTAAGCAAGGATTCATTTCCTTCAACTTCCAACTTAAATATCTGTTTGTTTCAACTGTTTCTTCAACTTTGATCATTCTTTTGAACTTCAGGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTAAACCACCTGAGGAATTTTGTGTATCAGCAAACACTGCCCAGCTGATGCTTCTGTATTTTGCATTTCTTCTAATGGCGATCGGAGCTGGGGGAATTCGACCATGCTCTTTGGCGTTTGGGGCAGATCAACTAGAAAAGCCAGGCAACCCCAAAAACCAAAGAACTATGCAAAGCTTCTTCAACTGGTACTATGCTACTGTTGGAATTTCAGTCACCATTTCAGTGGTATTTATGGTTTATCTCCAGAACGCTGCCGGTTGGGTTGTGGGTTACGGAGTTCCTGTTGGACTTATGGTGTTATCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATATTAAACTGATGGCAAACAAGAGTTTGCTTAGCAGTTTGGCTCAGGTAATCGTTGCAGCTTGGAGAAATAGGAACTTGGAATTCCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGCTCAAAACTCGTCACCCCAACGCCGAAATTGAGGTATTTTAAGAGTCAAAACGTTTCACAATTGTTGCTCACTACGCTTTTATAGTTTCTAACATGATCAAAGTTGATTCTTCTATCTTGTTTTAAGGTTTCTAAACAAGGCTTGCATAATAAGAAACAAAGAAACAGACATGGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACAATTCAACGAGTAGAGGAACTAAAAGCACTAATCAGATTACTTCCCATTTGGTCTACTGGCATTATAATCAGCGCCACCATCAACCAATTCACCTTCTCCGCTCTCCAAGCAAACACAATGGATAGACACATAACACCCCACTTCCAATTCCCAGCAGCTTCCTTTGCCGTCTTCACAATCCTAACCCTAACAATCTGGGTCGCAATATACGACCAAATCATCGTCCCACTACTAACGAAATTCACCAAAAAATCAAATGGACTAACTTTAAAGCAGAGAATGGGAATCGGACTCGCAATCTCATGCTTGGCCTCAGTAGTTTCAGCAGAAATCGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTTTAGCCAATGTACCGGAAGGAATCGTGAAGATGTCGGCAATGTGGCTGGTGCCACAGCACTGCCTGGCAGGGCTGGCTGAAGCGTTGAATGCGATAGGGCAGATTCAATTCTTCTACTCGCAGCTGCCGAGGAGTATGGCGAGCATTGCAGTAGCGATGTTCTCGCTGGGAATGGGCGGAGGAAGCTTGGTTGCAGCACTGATTGTGAGCGTGGTGAAGAAGAGGACGACGAAAAATGGGAATGTGGGATGGCTTTCGAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTTTTGAGCTTGATGGGTGTGGCTAATTTGTTGTATTATTTGATTTGCAGTTGGTTCTATGGAGATGAGGAGGAGGGAATGGAACAGAGTAGGGTTTGGGATGATAAGGAAGCCATTGAAGAAGAAGCCATCCTCGGTGCTTGA

mRNA sequence

ATGGAGAAGCAGAATTCAGAGGAAGTAATGGAAACCACTCAAAAGGTTACCAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTCATTTTAGCAAACGAGATATTTGAGAAGATTTCAAATATTGGTCTTCATGCAAATATGATATTTTACCTGATAAATGAGTATCACCTGAGTAGTGCTAAAGGAGCAATCGTCTTGTTTCTGTGGTCTGCACTGACAAACTTTTTGCCCATTGCCGGAGCTTTCCTCTCTGATTCTTACTTGGGTCGGTTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTCTTGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTAAACCACCTGAGGAATTTTGTGTATCAGCAAACACTGCCCAGCTGATGCTTCTGTATTTTGCATTTCTTCTAATGGCGATCGGAGCTGGGGGAATTCGACCATGCTCTTTGGCGTTTGGGGCAGATCAACTAGAAAAGCCAGGCAACCCCAAAAACCAAAGAACTATGCAAAGCTTCTTCAACTGGTACTATGCTACTGTTGGAATTTCAGTCACCATTTCAGTGGTATTTATGGTTTATCTCCAGAACGCTGCCGGTTGGGTTGTGGGTTACGGAGTTCCTGTTGGACTTATGGTGTTATCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATATTAAACTGATGGCAAACAAGAGTTTGCTTAGCAGTTTGGCTCAGGTAATCGTTGCAGCTTGGAGAAATAGGAACTTGGAATTCCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGCTCAAAACTCGTCACCCCAACGCCGAAATTGAGGTTTCTAAACAAGGCTTGCATAATAAGAAACAAAGAAACAGACATGGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACAATTCAACGAGTAGAGGAACTAAAAGCACTAATCAGATTACTTCCCATTTGGTCTACTGGCATTATAATCAGCGCCACCATCAACCAATTCACCTTCTCCGCTCTCCAAGCAAACACAATGGATAGACACATAACACCCCACTTCCAATTCCCAGCAGCTTCCTTTGCCGTCTTCACAATCCTAACCCTAACAATCTGGGTCGCAATATACGACCAAATCATCGTCCCACTACTAACGAAATTCACCAAAAAATCAAATGGACTAACTTTAAAGCAGAGAATGGGAATCGGACTCGCAATCTCATGCTTGGCCTCAGTAGTTTCAGCAGAAATCGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTTTAGCCAATGTACCGGAAGGAATCGTGAAGATGTCGGCAATGTGGCTGGTGCCACAGCACTGCCTGGCAGGGCTGGCTGAAGCGTTGAATGCGATAGGGCAGATTCAATTCTTCTACTCGCAGCTGCCGAGGAGTATGGCGAGCATTGCAGTAGCGATGTTCTCGCTGGGAATGGGCGGAGGAAGCTTGGTTGCAGCACTGATTGTGAGCGTGGTGAAGAAGAGGACGACGAAAAATGGGAATGTGGGATGGCTTTCGAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTTTTGAGCTTGATGGGTGTGGCTAATTTGTTGTATTATTTGATTTGCAGTTGGTTCTATGGAGATGAGGAGGAGGGAATGGAACAGAGTAGGGTTTGGGATGATAAGGAAGCCATTGAAGAAGAAGCCATCCTCGGTGCTTGA

Coding sequence (CDS)

ATGGAGAAGCAGAATTCAGAGGAAGTAATGGAAACCACTCAAAAGGTTACCAGAAAAAAGGGTGGTCTTCGAACTATGCCTTTCATTTTAGCAAACGAGATATTTGAGAAGATTTCAAATATTGGTCTTCATGCAAATATGATATTTTACCTGATAAATGAGTATCACCTGAGTAGTGCTAAAGGAGCAATCGTCTTGTTTCTGTGGTCTGCACTGACAAACTTTTTGCCCATTGCCGGAGCTTTCCTCTCTGATTCTTACTTGGGTCGGTTTAAGGTCATCGCCATGGGGACAGTGGTTACACTTCTTGGAATGGTGGTTTTATGGTTAACAGCCATATTTCCAAAAGCAAGGCCTCCACACTGTAAACCACCTGAGGAATTTTGTGTATCAGCAAACACTGCCCAGCTGATGCTTCTGTATTTTGCATTTCTTCTAATGGCGATCGGAGCTGGGGGAATTCGACCATGCTCTTTGGCGTTTGGGGCAGATCAACTAGAAAAGCCAGGCAACCCCAAAAACCAAAGAACTATGCAAAGCTTCTTCAACTGGTACTATGCTACTGTTGGAATTTCAGTCACCATTTCAGTGGTATTTATGGTTTATCTCCAGAACGCTGCCGGTTGGGTTGTGGGTTACGGAGTTCCTGTTGGACTTATGGTGTTATCCACAGCCATGTTCTTTCTTGGCTCTTCATTGTATATTAAACTGATGGCAAACAAGAGTTTGCTTAGCAGTTTGGCTCAGGTAATCGTTGCAGCTTGGAGAAATAGGAACTTGGAATTCCCCCCACAAGCTTCTGATAAATGGTTTTACCATAAAGGCTCAAAACTCGTCACCCCAACGCCGAAATTGAGGTTTCTAAACAAGGCTTGCATAATAAGAAACAAAGAAACAGACATGGATTCTAATGGAATGGCCAAATTCCCATGGAGATTAAGCACAATTCAACGAGTAGAGGAACTAAAAGCACTAATCAGATTACTTCCCATTTGGTCTACTGGCATTATAATCAGCGCCACCATCAACCAATTCACCTTCTCCGCTCTCCAAGCAAACACAATGGATAGACACATAACACCCCACTTCCAATTCCCAGCAGCTTCCTTTGCCGTCTTCACAATCCTAACCCTAACAATCTGGGTCGCAATATACGACCAAATCATCGTCCCACTACTAACGAAATTCACCAAAAAATCAAATGGACTAACTTTAAAGCAGAGAATGGGAATCGGACTCGCAATCTCATGCTTGGCCTCAGTAGTTTCAGCAGAAATCGAGAGAAAACGAAGAAACAGAGCAATTCATGAAGGTTTAGCCAATGTACCGGAAGGAATCGTGAAGATGTCGGCAATGTGGCTGGTGCCACAGCACTGCCTGGCAGGGCTGGCTGAAGCGTTGAATGCGATAGGGCAGATTCAATTCTTCTACTCGCAGCTGCCGAGGAGTATGGCGAGCATTGCAGTAGCGATGTTCTCGCTGGGAATGGGCGGAGGAAGCTTGGTTGCAGCACTGATTGTGAGCGTGGTGAAGAAGAGGACGACGAAAAATGGGAATGTGGGATGGCTTTCGAATAATCTGAACCAGGGGCATTATGATTACTACTACTGGGTTTTGAGCTTGATGGGTGTGGCTAATTTGTTGTATTATTTGATTTGCAGTTGGTTCTATGGAGATGAGGAGGAGGGAATGGAACAGAGTAGGGTTTGGGATGATAAGGAAGCCATTGAAGAAGAAGCCATCCTCGGTGCTTGA

Protein sequence

MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDMDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA
Homology
BLAST of Bhi02G000959 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.6e-162
Identity = 288/576 (50.00%), Postives = 405/576 (70.31%), Query Frame = 0

Query: 13  TQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSAL 72
           T +  + KGG+ TMPFI+ANE FEK+++ GL  NMI YLI +Y    AKG  VLF+WSA 
Sbjct: 15  TNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAA 74

Query: 73  TNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPE--EFCV 132
           +NF P+ GAFLSDSYLGRF  I++ ++ + LGMV+LWLTA+ P+ +P  C P      C 
Sbjct: 75  SNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCG 134

Query: 133 SANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVG 192
           S+  +QL LLY AF L++IG+GGIRPCSLAFGADQL+   NPKN+R ++SFF WYYA+  
Sbjct: 135 SSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSA 194

Query: 193 ISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQV 252
           ++V I+   +VY+Q   GW +G+GVP  LM+++  +F L S LY+     KSL + LAQ 
Sbjct: 195 VAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQA 254

Query: 253 IVAAWRNRNLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDMDSNGMAK 312
           IVAA++ R L  P    + D +++ K S++  P+ KLRFLNKAC+I N+E ++ S+G A 
Sbjct: 255 IVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFAL 314

Query: 313 FPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHITPH---FQF 372
            PWRL T  +VEELKALI+++PIWSTGI++S   +Q +F  LQA +MDR ++ H   FQ 
Sbjct: 315 NPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQV 374

Query: 373 PAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAE 432
           PA SF +FTI+ L +WV +YD+ ++PL +K   +   L++K RMG+GL +S LA  +SA 
Sbjct: 375 PAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAM 434

Query: 433 IERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 492
           +E  RR +AI +G AN    +V +SAMWLVPQ+ L GLAEAL AIGQ +FFY++ P+SM+
Sbjct: 435 VESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMS 494

Query: 493 SIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVA 552
           SIA ++F LGM   SL+A+++++ V + T++NG   W+S+N+N+GHY+YYYWVL++M   
Sbjct: 495 SIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFI 554

Query: 553 NLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAI 582
           N++YY+ICSW YG   + +   RV   +E  EEE I
Sbjct: 555 NVIYYVICSWSYGPLVDQVRNGRVNGVRE--EEELI 588

BLAST of Bhi02G000959 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 557.8 bits (1436), Expect = 1.1e-158
Identity = 287/585 (49.06%), Postives = 407/585 (69.57%), Query Frame = 0

Query: 2   EKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAK 61
           ++  S+E ++      R KGGL TMPFI+ANE FEK+++ GL  NMI YL+++Y L   K
Sbjct: 6   DQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVK 65

Query: 62  GAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPH 121
           G  VLF+W A TNF+P+ GAFLSDSYLGRF  I + ++ +LLGMVVLWLTA+ P+ +P  
Sbjct: 66  GQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSP 125

Query: 122 C-KPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 181
           C       C SA ++QL LLY AF L++IG+GGIRPCSLAFGADQL+   NPKN+R ++S
Sbjct: 126 CVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLES 185

Query: 182 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 241
           FF WYYA+  ++V I+   +VY+Q+  GW +G+G+P  LM+L+  +F   S LY+K   +
Sbjct: 186 FFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVS 245

Query: 242 KSLLSSLAQVIVAAWRNRNLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKE 301
           KSL + LAQV+ AA+  RNL  P    + D ++  K S+L  P+ KLRFLNKAC I N++
Sbjct: 246 KSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRD 305

Query: 302 TDMDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRH 361
            D+ S+G+A   WRL T  +VE+LKAL++++P+WSTGI++S  ++Q +F  LQA +MDR 
Sbjct: 306 EDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRR 365

Query: 362 ITPH--FQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAIS 421
           ++ +  FQ PA SF +FTI+ L  WV +YD+ I+PL +K   +   + +K RMG+GL IS
Sbjct: 366 LSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFIS 425

Query: 422 CLASVVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFF 481
            LA  VSA +E  RR  AI +GLAN     V +SAMWLVPQ+ L GLAEAL  IGQ +FF
Sbjct: 426 FLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFF 485

Query: 482 YSQLPRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYY 541
           Y++ P+SM+SIA ++F LGM   +++A++I++ V K ++K GNV W+ +N+N+GHYDYYY
Sbjct: 486 YTEFPKSMSSIAASLFGLGMAVANILASVILNAV-KNSSKQGNVSWIEDNINKGHYDYYY 545

Query: 542 WVLSLMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAI 582
           WVL+++   N++YY++CSW YG   + +   +V +     EEE I
Sbjct: 546 WVLAILSFVNVIYYVVCSWSYGPTVDQVRNDKV-NGMRKEEEEVI 588

BLAST of Bhi02G000959 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.7e-116
Identity = 247/592 (41.72%), Postives = 360/592 (60.81%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKV----TRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYH 60
           ME+Q+  ++ E  +++     R KGGL TMPFI ANEI EK++ +G HANMI YL  + H
Sbjct: 1   MEEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLH 60

Query: 61  LSSAKGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPK 120
           L   K A  L  ++  ++  P+ GAF++DS+ GRF  I   +++  +GM +L ++AI P 
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 121 ARPPHCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQR 180
            RPP CK  EE CV A+TAQL +LY A LL A+G+GGIRPC +AFGADQ ++  +P    
Sbjct: 121 LRPPPCK-GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDE-SDPNQTT 180

Query: 181 TMQSFFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIK 240
              ++FNWYY  +G +V ++V  +V++Q+  GW +G G+P   M LS   F  G  LY  
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 241 LMANKSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHK-------GSKLVTPTPKLRFLN 300
           L+   S  + L QV VAA+R R L      S  +F  +       G KL T T  + FL+
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKL-THTKHMSFLD 300

Query: 301 KACIIRNKETDMDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGI-IISATINQFTFS 360
           KA I+   E D    G     WRLST+ RVEELK++IR+ PI ++GI +I+A   Q TFS
Sbjct: 301 KAAIV--TEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFS 360

Query: 361 ALQANTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQR 420
             QA TM+RH+T  FQ PA S +VFT + +   +  YD++ V +  KFT    G+T   R
Sbjct: 361 LQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHR 420

Query: 421 MGIGLAISCLASVVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALN 480
           MGIG  IS +A++V+  +E KR++ AI  GL + P  IV +S +WL+PQ+ L G+AEA  
Sbjct: 421 MGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFM 480

Query: 481 AIGQIQFFYSQLPRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWL-SNNL 540
           +IG ++FFY Q P SM S A A+F + +  G+ V+ L+V++V K + K     WL  NNL
Sbjct: 481 SIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNL 540

Query: 541 NQGHYDYYYWVLSLMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEE 580
           N+G  +Y+YW+++++   NL+YYL C+  Y  +   +  S+  +D   ++EE
Sbjct: 541 NRGRLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSK--EDSSPVKEE 585

BLAST of Bhi02G000959 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-114
Identity = 227/580 (39.14%), Postives = 346/580 (59.66%), Query Frame = 0

Query: 14  QKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSALT 73
           +KV +K GG R + FIL NE  E++ +IGL AN + YL   +HL     A V+ +WS  T
Sbjct: 46  EKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFT 105

Query: 74  NFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPEEF-CVSA 133
           N  P+ GA++SD+Y+GRFK IA  +  TLLG++ + LTA FP+  P  C   +   C   
Sbjct: 106 NLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGP 165

Query: 134 NTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVGIS 193
           N  Q+ +L      +++G+GGIRPCS+ FG DQ ++    +  + + SFFNWYY T  + 
Sbjct: 166 NKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQ-RTEEGVKGVASFFNWYYMTFTVV 225

Query: 194 VTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQVIV 253
           + I+   +VY+Q+   W++G+ +P GLM L+  MFF G   Y+ +    S+ S +AQVIV
Sbjct: 226 LIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIV 285

Query: 254 AAWRNRNLEFPPQASDKWFYH----KGSKL--VTPTPKLRFLNKACIIRNKETDMDSNGM 313
           AA + R L+ P +      Y+    K S L  +  + + R L+KA ++   E D+   G 
Sbjct: 286 AARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGP 345

Query: 314 AKFPWRLSTIQRVEELKALIRLLPIWSTGII-ISATINQFTFSALQANTMDRHITPHFQF 373
               WRL ++Q VEE+K LIR++PIWS GII ++A   Q TF+  QA  MDR++ P F+ 
Sbjct: 346 PADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEI 405

Query: 374 PAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAE 433
           PA S +V ++LT+ I++  YD++ VP + + T   +G+TL QR+G G+  +  + +V+  
Sbjct: 406 PAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGI 465

Query: 434 IERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 493
           +ER RR R+I+ G    P G+  MS  WL PQ  L GL EA N IGQI+FF SQ P  M 
Sbjct: 466 VERMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 525

Query: 494 SIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVA 553
           SIA ++FSL   G S +++ +V+VV K +  +    WL+ NLN G  DY+Y++++++GV 
Sbjct: 526 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVV 585

Query: 554 NLLYYLICSWFY----GDEEEGMEQSRVWDDKEAIEEEAI 582
           NL+Y+  C+  Y    G   E  E+ +  DD E   ++++
Sbjct: 586 NLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEMTSKKSM 619

BLAST of Bhi02G000959 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 375.9 bits (964), Expect = 5.7e-104
Identity = 214/549 (38.98%), Postives = 324/549 (59.02%), Query Frame = 0

Query: 18  RKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSALTNFLP 77
           +K GG R + FIL NE  EK+ +IG+ AN + YL N +H+   +   V +LW  LTNF P
Sbjct: 13  KKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAP 72

Query: 78  IAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPE-EFCVSANTAQ 137
           + GA +SD+Y+GRFK IA  ++ ++LG++ + LTA  P+  PP C  P  + C   N  Q
Sbjct: 73  LLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQ 132

Query: 138 LMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVGISVTIS 197
           L +L+     ++IG+GGIRPCS+ FG DQ ++    +  + + SFFNWYY T+ + +  S
Sbjct: 133 LGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQ-RTEQGLKGVASFFNWYYLTLTMVLIFS 192

Query: 198 VVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQVIVAAWR 257
              +VYLQ  + WV+G+ +P  LM  +  +FF+G   Y+ +    S+ S +A+VIVAA +
Sbjct: 193 HTVVVYLQTVS-WVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARK 252

Query: 258 NRNLEFPPQASDKWFYHKG-------SKLVTPTPKLRFLNKACIIRNKETDMDSNGMAKF 317
            R+L+          Y++        SKL   T + +FL+KA +I   + D+ S G+   
Sbjct: 253 KRDLKISLVDDGTEEYYEPPVKPGVLSKLPL-TDQFKFLDKAAVI--LDGDLTSEGVPAN 312

Query: 318 PWRLSTIQRVEELKALIRLLPIWSTGII-ISATINQFTFSALQANTMDRHITPHFQFPAA 377
            WRL +IQ VEE+K LIR++P+WS GII I A   Q TF   QA  MDRH+ PHF+ PAA
Sbjct: 313 KWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAA 372

Query: 378 SFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAEIER 437
           S  V + +T+ IWV IY+ ++VP L +  K    +TL QRMGIG+  + L+   +  +E 
Sbjct: 373 SITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVEG 432

Query: 438 KRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASIA 497
            RR RA           + +MS  WL     L GL E+ N IG I+FF SQ P  M SIA
Sbjct: 433 VRRTRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIA 492

Query: 498 VAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVANLL 557
            ++F L     + +++L+V+ V K +    +  WL+ +L++G  DY+Y++++++GV NL+
Sbjct: 493 NSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLV 545

BLAST of Bhi02G000959 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 6.5e-161
Identity = 288/576 (50.00%), Postives = 405/576 (70.31%), Query Frame = 0

Query: 13  TQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSAL 72
           T +  + KGG+ TMPFI+ANE FEK+++ GL  NMI YLI +Y    AKG  VLF+WSA 
Sbjct: 15  TNEGKKTKGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAA 74

Query: 73  TNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPE--EFCV 132
           +NF P+ GAFLSDSYLGRF  I++ ++ + LGMV+LWLTA+ P+ +P  C P      C 
Sbjct: 75  SNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCG 134

Query: 133 SANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVG 192
           S+  +QL LLY AF L++IG+GGIRPCSLAFGADQL+   NPKN+R ++SFF WYYA+  
Sbjct: 135 SSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSA 194

Query: 193 ISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQV 252
           ++V I+   +VY+Q   GW +G+GVP  LM+++  +F L S LY+     KSL + LAQ 
Sbjct: 195 VAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQA 254

Query: 253 IVAAWRNRNLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETDMDSNGMAK 312
           IVAA++ R L  P    + D +++ K S++  P+ KLRFLNKAC+I N+E ++ S+G A 
Sbjct: 255 IVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFAL 314

Query: 313 FPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHITPH---FQF 372
            PWRL T  +VEELKALI+++PIWSTGI++S   +Q +F  LQA +MDR ++ H   FQ 
Sbjct: 315 NPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQV 374

Query: 373 PAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAE 432
           PA SF +FTI+ L +WV +YD+ ++PL +K   +   L++K RMG+GL +S LA  +SA 
Sbjct: 375 PAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAM 434

Query: 433 IERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 492
           +E  RR +AI +G AN    +V +SAMWLVPQ+ L GLAEAL AIGQ +FFY++ P+SM+
Sbjct: 435 VESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMS 494

Query: 493 SIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVA 552
           SIA ++F LGM   SL+A+++++ V + T++NG   W+S+N+N+GHY+YYYWVL++M   
Sbjct: 495 SIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFI 554

Query: 553 NLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAI 582
           N++YY+ICSW YG   + +   RV   +E  EEE I
Sbjct: 555 NVIYYVICSWSYGPLVDQVRNGRVNGVRE--EEELI 588

BLAST of Bhi02G000959 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 557.8 bits (1436), Expect = 1.5e-157
Identity = 287/585 (49.06%), Postives = 407/585 (69.57%), Query Frame = 0

Query: 2   EKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAK 61
           ++  S+E ++      R KGGL TMPFI+ANE FEK+++ GL  NMI YL+++Y L   K
Sbjct: 6   DQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVK 65

Query: 62  GAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPH 121
           G  VLF+W A TNF+P+ GAFLSDSYLGRF  I + ++ +LLGMVVLWLTA+ P+ +P  
Sbjct: 66  GQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSP 125

Query: 122 C-KPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 181
           C       C SA ++QL LLY AF L++IG+GGIRPCSLAFGADQL+   NPKN+R ++S
Sbjct: 126 CVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLES 185

Query: 182 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 241
           FF WYYA+  ++V I+   +VY+Q+  GW +G+G+P  LM+L+  +F   S LY+K   +
Sbjct: 186 FFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVS 245

Query: 242 KSLLSSLAQVIVAAWRNRNLEFPP--QASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKE 301
           KSL + LAQV+ AA+  RNL  P    + D ++  K S+L  P+ KLRFLNKAC I N++
Sbjct: 246 KSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRD 305

Query: 302 TDMDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRH 361
            D+ S+G+A   WRL T  +VE+LKAL++++P+WSTGI++S  ++Q +F  LQA +MDR 
Sbjct: 306 EDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRR 365

Query: 362 ITPH--FQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAIS 421
           ++ +  FQ PA SF +FTI+ L  WV +YD+ I+PL +K   +   + +K RMG+GL IS
Sbjct: 366 LSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFIS 425

Query: 422 CLASVVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFF 481
            LA  VSA +E  RR  AI +GLAN     V +SAMWLVPQ+ L GLAEAL  IGQ +FF
Sbjct: 426 FLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFF 485

Query: 482 YSQLPRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYY 541
           Y++ P+SM+SIA ++F LGM   +++A++I++ V K ++K GNV W+ +N+N+GHYDYYY
Sbjct: 486 YTEFPKSMSSIAASLFGLGMAVANILASVILNAV-KNSSKQGNVSWIEDNINKGHYDYYY 545

Query: 542 WVLSLMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAI 582
           WVL+++   N++YY++CSW YG   + +   +V +     EEE I
Sbjct: 546 WVLAILSFVNVIYYVVCSWSYGPTVDQVRNDKV-NGMRKEEEEVI 588

BLAST of Bhi02G000959 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.4e-115
Identity = 247/592 (41.72%), Postives = 360/592 (60.81%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKV----TRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYH 60
           ME+Q+  ++ E  +++     R KGGL TMPFI ANEI EK++ +G HANMI YL  + H
Sbjct: 1   MEEQSKNKISEEEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLH 60

Query: 61  LSSAKGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPK 120
           L   K A  L  ++  ++  P+ GAF++DS+ GRF  I   +++  +GM +L ++AI P 
Sbjct: 61  LPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPT 120

Query: 121 ARPPHCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQR 180
            RPP CK  EE CV A+TAQL +LY A LL A+G+GGIRPC +AFGADQ ++  +P    
Sbjct: 121 LRPPPCK-GEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDE-SDPNQTT 180

Query: 181 TMQSFFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIK 240
              ++FNWYY  +G +V ++V  +V++Q+  GW +G G+P   M LS   F  G  LY  
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 241 LMANKSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHK-------GSKLVTPTPKLRFLN 300
           L+   S  + L QV VAA+R R L      S  +F  +       G KL T T  + FL+
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKL-THTKHMSFLD 300

Query: 301 KACIIRNKETDMDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGI-IISATINQFTFS 360
           KA I+   E D    G     WRLST+ RVEELK++IR+ PI ++GI +I+A   Q TFS
Sbjct: 301 KAAIV--TEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFS 360

Query: 361 ALQANTMDRHITPHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQR 420
             QA TM+RH+T  FQ PA S +VFT + +   +  YD++ V +  KFT    G+T   R
Sbjct: 361 LQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHR 420

Query: 421 MGIGLAISCLASVVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALN 480
           MGIG  IS +A++V+  +E KR++ AI  GL + P  IV +S +WL+PQ+ L G+AEA  
Sbjct: 421 MGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFM 480

Query: 481 AIGQIQFFYSQLPRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWL-SNNL 540
           +IG ++FFY Q P SM S A A+F + +  G+ V+ L+V++V K + K     WL  NNL
Sbjct: 481 SIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNL 540

Query: 541 NQGHYDYYYWVLSLMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEE 580
           N+G  +Y+YW+++++   NL+YYL C+  Y  +   +  S+  +D   ++EE
Sbjct: 541 NRGRLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSK--EDSSPVKEE 585

BLAST of Bhi02G000959 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.0e-113
Identity = 227/580 (39.14%), Postives = 346/580 (59.66%), Query Frame = 0

Query: 14  QKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSALT 73
           +KV +K GG R + FIL NE  E++ +IGL AN + YL   +HL     A V+ +WS  T
Sbjct: 46  EKVEKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFT 105

Query: 74  NFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPEEF-CVSA 133
           N  P+ GA++SD+Y+GRFK IA  +  TLLG++ + LTA FP+  P  C   +   C   
Sbjct: 106 NLTPLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGP 165

Query: 134 NTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVGIS 193
           N  Q+ +L      +++G+GGIRPCS+ FG DQ ++    +  + + SFFNWYY T  + 
Sbjct: 166 NKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQ-RTEEGVKGVASFFNWYYMTFTVV 225

Query: 194 VTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQVIV 253
           + I+   +VY+Q+   W++G+ +P GLM L+  MFF G   Y+ +    S+ S +AQVIV
Sbjct: 226 LIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIV 285

Query: 254 AAWRNRNLEFPPQASDKWFYH----KGSKL--VTPTPKLRFLNKACIIRNKETDMDSNGM 313
           AA + R L+ P +      Y+    K S L  +  + + R L+KA ++   E D+   G 
Sbjct: 286 AARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGP 345

Query: 314 AKFPWRLSTIQRVEELKALIRLLPIWSTGII-ISATINQFTFSALQANTMDRHITPHFQF 373
               WRL ++Q VEE+K LIR++PIWS GII ++A   Q TF+  QA  MDR++ P F+ 
Sbjct: 346 PADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEI 405

Query: 374 PAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAE 433
           PA S +V ++LT+ I++  YD++ VP + + T   +G+TL QR+G G+  +  + +V+  
Sbjct: 406 PAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGI 465

Query: 434 IERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMA 493
           +ER RR R+I+ G    P G+  MS  WL PQ  L GL EA N IGQI+FF SQ P  M 
Sbjct: 466 VERMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 525

Query: 494 SIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVA 553
           SIA ++FSL   G S +++ +V+VV K +  +    WL+ NLN G  DY+Y++++++GV 
Sbjct: 526 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVV 585

Query: 554 NLLYYLICSWFY----GDEEEGMEQSRVWDDKEAIEEEAI 582
           NL+Y+  C+  Y    G   E  E+ +  DD E   ++++
Sbjct: 586 NLVYFWYCARGYRYKVGLPIEDFEEDKSSDDVEMTSKKSM 619

BLAST of Bhi02G000959 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 8.1e-103
Identity = 214/549 (38.98%), Postives = 324/549 (59.02%), Query Frame = 0

Query: 18  RKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSAKGAIVLFLWSALTNFLP 77
           +K GG R + FIL NE  EK+ +IG+ AN + YL N +H+   +   V +LW  LTNF P
Sbjct: 13  KKLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAP 72

Query: 78  IAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPPHCKPPE-EFCVSANTAQ 137
           + GA +SD+Y+GRFK IA  ++ ++LG++ + LTA  P+  PP C  P  + C   N  Q
Sbjct: 73  LLGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQ 132

Query: 138 LMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQSFFNWYYATVGISVTIS 197
           L +L+     ++IG+GGIRPCS+ FG DQ ++    +  + + SFFNWYY T+ + +  S
Sbjct: 133 LGILFLGLGFLSIGSGGIRPCSIPFGVDQFDQ-RTEQGLKGVASFFNWYYLTLTMVLIFS 192

Query: 198 VVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMANKSLLSSLAQVIVAAWR 257
              +VYLQ  + WV+G+ +P  LM  +  +FF+G   Y+ +    S+ S +A+VIVAA +
Sbjct: 193 HTVVVYLQTVS-WVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARK 252

Query: 258 NRNLEFPPQASDKWFYHKG-------SKLVTPTPKLRFLNKACIIRNKETDMDSNGMAKF 317
            R+L+          Y++        SKL   T + +FL+KA +I   + D+ S G+   
Sbjct: 253 KRDLKISLVDDGTEEYYEPPVKPGVLSKLPL-TDQFKFLDKAAVI--LDGDLTSEGVPAN 312

Query: 318 PWRLSTIQRVEELKALIRLLPIWSTGII-ISATINQFTFSALQANTMDRHITPHFQFPAA 377
            WRL +IQ VEE+K LIR++P+WS GII I A   Q TF   QA  MDRH+ PHF+ PAA
Sbjct: 313 KWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAA 372

Query: 378 SFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLASVVSAEIER 437
           S  V + +T+ IWV IY+ ++VP L +  K    +TL QRMGIG+  + L+   +  +E 
Sbjct: 373 SITVISYITIGIWVPIYEHLLVPFLWRMRKFR--VTLLQRMGIGIVFAILSMFTAGFVEG 432

Query: 438 KRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQLPRSMASIA 497
            RR RA           + +MS  WL     L GL E+ N IG I+FF SQ P  M SIA
Sbjct: 433 VRRTRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIA 492

Query: 498 VAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLSLMGVANLL 557
            ++F L     + +++L+V+ V K +    +  WL+ +L++G  DY+Y++++++GV NL+
Sbjct: 493 NSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLV 545

BLAST of Bhi02G000959 vs. NCBI nr
Match: XP_038879889.1 (protein NRT1/ PTR FAMILY 1.2-like [Benincasa hispida])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 584/584 (100.00%), Postives = 584/584 (100.00%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA
Sbjct: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS
Sbjct: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN
Sbjct: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD
Sbjct: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT
Sbjct: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS
Sbjct: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA
Sbjct: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 584

BLAST of Bhi02G000959 vs. NCBI nr
Match: XP_008462682.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo] >KAA0046539.1 protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo var. makuwa] >TYK28719.1 protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1054.3 bits (2725), Expect = 3.9e-304
Identity = 531/584 (90.92%), Postives = 557/584 (95.38%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           ME QNSE+ METTQKV RKKGGLRTMPFI+ANE FEKISN+GLHANMIFYL+NEYH+  A
Sbjct: 1   MENQNSEK-METTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPI GAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HC+PP EFCVSANTAQLMLLYFAFLLMA GAGGIRPCSLAFGADQLE PGNPKNQRTMQS
Sbjct: 121 HCRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYA+VGISVTISVVFMVYLQNAAGW+VG+GVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLLSSL QVIVAAWRNR+LEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD
Sbjct: 241 KSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGIIISATINQFTFS+LQA+TMDRHIT
Sbjct: 301 VDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVF ILTLTIWVAIYDQIIVPLL KFTKKSNGLTLKQRMGIGL +SCL S
Sbjct: 361 PHFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           +VSA IERKRRNRAIHEGLANVP GIV+MSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 IVSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSLVAAL VSVVKKRTTKNGNVGWLS+NLNQGHYDYYYW+LS
Sbjct: 481 PRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANLL+YLI SWFYGDE+EG EQSR+WDDKEAIE E    A
Sbjct: 541 LMGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of Bhi02G000959 vs. NCBI nr
Match: KAE8652969.1 (hypothetical protein Csa_017739 [Cucumis sativus])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.5e-303
Identity = 533/580 (91.90%), Postives = 556/580 (95.86%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           ME QNSE+ METTQKV RKKGGLRTMPFI+ANEIFEKISN+GLHANMIFYL+NEYHL SA
Sbjct: 1   MENQNSEK-METTQKVERKKGGLRTMPFIIANEIFEKISNVGLHANMIFYLMNEYHLDSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTN LPI GAFLSDSYLGRFKVIAMGTVVTL+GMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNLLPIGGAFLSDSYLGRFKVIAMGTVVTLVGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HC P  EFCVSANTAQLMLLYFAFLLMA GAGGIRPCSLAFGADQLE PGNP+NQRTMQS
Sbjct: 121 HCTPAGEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPRNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYA+VGISVTISVVFMVYLQNA GWVVG+GVPVGLM+ STAMFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYASVGISVTISVVFMVYLQNATGWVVGFGVPVGLMLFSTAMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLLSSL QVIVAAWRNR+LEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRN ETD
Sbjct: 241 KSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNIETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGI+ISATINQFTF +LQA+TMDRHIT
Sbjct: 301 LDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQFTFYSLQASTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLL KFTKKSNGLTLKQRMGIGL ISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           +VSAEIERKRRNRAIHEGLANVP GIV++SAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 IVSAEIERKRRNRAIHEGLANVPGGIVQLSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSLVAAL VSVVKKRT KNG  GWLSNNLNQGHYDYYYWVLS
Sbjct: 481 PRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTKKNGK-GWLSNNLNQGHYDYYYWVLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEA 581
           LMGVANLLYYLICSWFYGDE+EGMEQSR+WDDKEAIE EA
Sbjct: 541 LMGVANLLYYLICSWFYGDEKEGMEQSRIWDDKEAIEAEA 578

BLAST of Bhi02G000959 vs. NCBI nr
Match: KAG6589921.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1018.1 bits (2631), Expect = 3.1e-293
Identity = 516/585 (88.21%), Postives = 546/585 (93.33%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           MEK+ SE+ METTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHL SA
Sbjct: 1   MEKEPSEK-METTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHTNMIFYLTNEYHLDSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPI GAFLSDSYLGRFKVIAMGT+VTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCK P EFCVSAN AQLMLLYFAFLLM+IGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS
Sbjct: 121 HCKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVG+SVTISV+FMVYLQNAAGWVVGYGVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMMFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLL SLAQVIVAAW+NR+LE PPQ SDKWFYHKGSKLV PTPKLRFLNKAC+IRNKETD
Sbjct: 241 KSLLRSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVNPTPKLRFLNKACMIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRL+TIQRVEELKA+IR+LPIWSTGI+ISATI+Q+TFSALQA TMDR IT
Sbjct: 301 VDSNGMAKFPWRLTTIQRVEELKAVIRVLPIWSTGIVISATISQYTFSALQAITMDRQIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLT WVAIYDQIIV LL KFTK+SNGLT KQRMGIGLAISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
            VSAEIERKRRNRA HEGLANVP GIVKMSAMWLVPQHCLAGLAEA +AIGQIQFFYSQL
Sbjct: 421 AVSAEIERKRRNRAFHEGLANVPGGIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSL+AALIVSVVKK+TTKNGN GWLSNN NQGHYDYYYWVL+
Sbjct: 481 PRSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLA 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVW-DDKEAIEEEAILGA 585
           LMGVANL YYLICSW YGDE+E ME+SR+W DDKEAIEE+    A
Sbjct: 541 LMGVANLFYYLICSWLYGDEKEIMEESRIWDDDKEAIEEDGTYNA 584

BLAST of Bhi02G000959 vs. NCBI nr
Match: XP_022987664.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita maxima])

HSP 1 Score: 1017.7 bits (2630), Expect = 4.0e-293
Identity = 515/584 (88.18%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           MEKQ SE+ METTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHL SA
Sbjct: 1   MEKQASEK-METTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHPNMIFYLTNEYHLDSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           K AIVLFLWSALTNFLP  GAFLSDSYLGRFKVIAMGT+VTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KAAIVLFLWSALTNFLPFVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCK P EFCVSAN AQLMLLYFAFLLM+IGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS
Sbjct: 121 HCKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVG+SVTISV+FMVYLQNAAGWVVGYGVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMLFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLL SLAQVIVAAW+NR+LE PPQ SDKWFYHKGSKL  PTPKLRFLNKAC+IRNKETD
Sbjct: 241 KSLLRSLAQVIVAAWKNRHLELPPQISDKWFYHKGSKLFIPTPKLRFLNKACMIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGI+ISATINQ+TFSALQA TMDR IT
Sbjct: 301 VDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQYTFSALQAITMDRQIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLT WVAIYDQIIV LL KFTK+SNGLT KQRMGIGLAISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
            VSAEIERKRRNRA HEGLANVP  IVKMSAMWLVPQHCLAGLAEA +AIGQIQFFYSQL
Sbjct: 421 AVSAEIERKRRNRAFHEGLANVPGAIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSL+AALIVSVVKK+TTKNGN GWLSNN NQGHYDYYYWVL+
Sbjct: 481 PRSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLT 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANL YYLICSW YGDE+E ME+SR+WDDKEAIEE+    A
Sbjct: 541 LMGVANLFYYLICSWLYGDEKEIMEESRIWDDKEAIEEDGTYNA 583

BLAST of Bhi02G000959 vs. ExPASy TrEMBL
Match: A0A5A7TZ76 (Protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00380 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.9e-304
Identity = 531/584 (90.92%), Postives = 557/584 (95.38%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           ME QNSE+ METTQKV RKKGGLRTMPFI+ANE FEKISN+GLHANMIFYL+NEYH+  A
Sbjct: 1   MENQNSEK-METTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPI GAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HC+PP EFCVSANTAQLMLLYFAFLLMA GAGGIRPCSLAFGADQLE PGNPKNQRTMQS
Sbjct: 121 HCRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYA+VGISVTISVVFMVYLQNAAGW+VG+GVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLLSSL QVIVAAWRNR+LEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD
Sbjct: 241 KSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGIIISATINQFTFS+LQA+TMDRHIT
Sbjct: 301 VDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVF ILTLTIWVAIYDQIIVPLL KFTKKSNGLTLKQRMGIGL +SCL S
Sbjct: 361 PHFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           +VSA IERKRRNRAIHEGLANVP GIV+MSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 IVSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSLVAAL VSVVKKRTTKNGNVGWLS+NLNQGHYDYYYW+LS
Sbjct: 481 PRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANLL+YLI SWFYGDE+EG EQSR+WDDKEAIE E    A
Sbjct: 541 LMGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of Bhi02G000959 vs. ExPASy TrEMBL
Match: A0A1S3CHG5 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103500984 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.9e-304
Identity = 531/584 (90.92%), Postives = 557/584 (95.38%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           ME QNSE+ METTQKV RKKGGLRTMPFI+ANE FEKISN+GLHANMIFYL+NEYH+  A
Sbjct: 1   MENQNSEK-METTQKVERKKGGLRTMPFIIANETFEKISNVGLHANMIFYLMNEYHMDKA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPI GAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIGGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HC+PP EFCVSANTAQLMLLYFAFLLMA GAGGIRPCSLAFGADQLE PGNPKNQRTMQS
Sbjct: 121 HCRPPSEFCVSANTAQLMLLYFAFLLMAAGAGGIRPCSLAFGADQLENPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYA+VGISVTISVVFMVYLQNAAGW+VG+GVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYASVGISVTISVVFMVYLQNAAGWIVGFGVPVGLMLFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLLSSL QVIVAAWRNR+LEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD
Sbjct: 241 KSLLSSLTQVIVAAWRNRHLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGIIISATINQFTFS+LQA+TMDRHIT
Sbjct: 301 VDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIIISATINQFTFSSLQASTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVF ILTLTIWVAIYDQIIVPLL KFTKKSNGLTLKQRMGIGL +SCL S
Sbjct: 361 PHFQFPAASFAVFAILTLTIWVAIYDQIIVPLLAKFTKKSNGLTLKQRMGIGLVVSCLTS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
           +VSA IERKRRNRAIHEGLANVP GIV+MSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 IVSAGIERKRRNRAIHEGLANVPGGIVQMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSLVAAL VSVVKKRTTKNGNVGWLS+NLNQGHYDYYYW+LS
Sbjct: 481 PRSMASIAVALFSLGMGGGSLVAALTVSVVKKRTTKNGNVGWLSDNLNQGHYDYYYWLLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANLL+YLI SWFYGDE+EG EQSR+WDDKEAIE E    A
Sbjct: 541 LMGVANLLHYLIFSWFYGDEKEGEEQSRIWDDKEAIEVEGTSNA 583

BLAST of Bhi02G000959 vs. ExPASy TrEMBL
Match: A0A6J1JJI0 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485150 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.9e-293
Identity = 515/584 (88.18%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           MEKQ SE+ METTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHL SA
Sbjct: 1   MEKQASEK-METTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHPNMIFYLTNEYHLDSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           K AIVLFLWSALTNFLP  GAFLSDSYLGRFKVIAMGT+VTLLGMVVLWLTAIFPKARPP
Sbjct: 61  KAAIVLFLWSALTNFLPFVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCK P EFCVSAN AQLMLLYFAFLLM+IGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS
Sbjct: 121 HCKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVG+SVTISV+FMVYLQNAAGWVVGYGVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 181 FFNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMLFSTVMFFLGSSLYVKLMAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLL SLAQVIVAAW+NR+LE PPQ SDKWFYHKGSKL  PTPKLRFLNKAC+IRNKETD
Sbjct: 241 KSLLRSLAQVIVAAWKNRHLELPPQISDKWFYHKGSKLFIPTPKLRFLNKACMIRNKETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRLSTIQRVEELKA+IR+LPIWSTGI+ISATINQ+TFSALQA TMDR IT
Sbjct: 301 VDSNGMAKFPWRLSTIQRVEELKAVIRVLPIWSTGIVISATINQYTFSALQAITMDRQIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLT WVAIYDQIIV LL KFTK+SNGLT KQRMGIGLAISCLAS
Sbjct: 361 PHFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
            VSAEIERKRRNRA HEGLANVP  IVKMSAMWLVPQHCLAGLAEA +AIGQIQFFYSQL
Sbjct: 421 AVSAEIERKRRNRAFHEGLANVPGAIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSL+AALIVSVVKK+TTKNGN GWLSNN NQGHYDYYYWVL+
Sbjct: 481 PRSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLT 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEEEAILGA 585
           LMGVANL YYLICSW YGDE+E ME+SR+WDDKEAIEE+    A
Sbjct: 541 LMGVANLFYYLICSWLYGDEKEIMEESRIWDDKEAIEEDGTYNA 583

BLAST of Bhi02G000959 vs. ExPASy TrEMBL
Match: A0A6J1HA09 (protein NRT1/ PTR FAMILY 1.1-like OS=Cucurbita moschata OX=3662 GN=LOC111461844 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.7e-293
Identity = 515/585 (88.03%), Postives = 545/585 (93.16%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           M K+ SE+ METTQK   KKGGLRTMPFI+ANEIFEKIS+ GLH NMIFYL NEYHL SA
Sbjct: 36  MGKEPSEK-METTQKEAGKKGGLRTMPFIMANEIFEKISSFGLHTNMIFYLTNEYHLDSA 95

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPI GAFLSDSYLGRFKVIAMGT+VTLLGMVVLWLTAIFPKARPP
Sbjct: 96  KGAIVLFLWSALTNFLPIVGAFLSDSYLGRFKVIAMGTLVTLLGMVVLWLTAIFPKARPP 155

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCK P EFCVSAN AQLMLLYFAFLLM+IGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS
Sbjct: 156 HCKSPGEFCVSANFAQLMLLYFAFLLMSIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 215

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVG+SVTISV+FMVYLQNAAGWVVGYGVPVGLM+ ST MFFLGSSLY+KLMAN
Sbjct: 216 FFNWYYATVGVSVTISVLFMVYLQNAAGWVVGYGVPVGLMMFSTVMFFLGSSLYVKLMAN 275

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLL SLAQVIVAAW+NR+LE PPQ SDKWFYHKGSKLV PTPKLRFLNKAC+IRNKETD
Sbjct: 276 KSLLRSLAQVIVAAWKNRHLELPPQTSDKWFYHKGSKLVNPTPKLRFLNKACMIRNKETD 335

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +DSNGMAKFPWRL+TIQRVEELKA+IR+LPIWSTGI+ISATI+Q+TFSALQA TMDR IT
Sbjct: 336 VDSNGMAKFPWRLTTIQRVEELKAVIRVLPIWSTGIVISATISQYTFSALQAITMDRQIT 395

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           PHFQFPAASFAVFTILTLT WVAIYDQIIV LL KFTK+SNGLT KQRMGIGLAISCLAS
Sbjct: 396 PHFQFPAASFAVFTILTLTFWVAIYDQIIVRLLAKFTKRSNGLTFKQRMGIGLAISCLAS 455

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
            VSAEIERKRRNRA HEGLANVP GIVKMSAMWLVPQHCLAGLAEA +AIGQIQFFYSQL
Sbjct: 456 AVSAEIERKRRNRAFHEGLANVPGGIVKMSAMWLVPQHCLAGLAEAFHAIGQIQFFYSQL 515

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSL+AALIVSVVKK+TTKNGN GWLSNN NQGHYDYYYWVL+
Sbjct: 516 PRSMASIAVALFSLGMGGGSLLAALIVSVVKKKTTKNGNAGWLSNNPNQGHYDYYYWVLA 575

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVW-DDKEAIEEEAILGA 585
           LMGVANL YYLICSW YGDE+E ME+SR+W DDKEAIEE+    A
Sbjct: 576 LMGVANLFYYLICSWLYGDEKEIMEESRIWDDDKEAIEEDGTYNA 619

BLAST of Bhi02G000959 vs. ExPASy TrEMBL
Match: A0A6J1CUG1 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111014453 PE=3 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.0e-286
Identity = 501/578 (86.68%), Postives = 536/578 (92.73%), Query Frame = 0

Query: 1   MEKQNSEEVMETTQKVTRKKGGLRTMPFILANEIFEKISNIGLHANMIFYLINEYHLSSA 60
           MEK +  E ME TQKV RKKGGLRTMPFI+ANEIFEKIS+IGLHANMIFYL NEYH  SA
Sbjct: 1   MEKPS--EKMEATQKVGRKKGGLRTMPFIMANEIFEKISSIGLHANMIFYLTNEYHFDSA 60

Query: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVIAMGTVVTLLGMVVLWLTAIFPKARPP 120
           KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVI+MGTVV+LLGMVVL LTAIFPKARPP
Sbjct: 61  KGAIVLFLWSALTNFLPIAGAFLSDSYLGRFKVISMGTVVSLLGMVVLLLTAIFPKARPP 120

Query: 121 HCKPPEEFCVSANTAQLMLLYFAFLLMAIGAGGIRPCSLAFGADQLEKPGNPKNQRTMQS 180
           HCK P E CV AN  QL+LL+F+FLLMAIGAGGIRPCSLAFGADQ EKPGNPKN+RT+QS
Sbjct: 121 HCKLPPEICVHANGGQLLLLFFSFLLMAIGAGGIRPCSLAFGADQFEKPGNPKNERTLQS 180

Query: 181 FFNWYYATVGISVTISVVFMVYLQNAAGWVVGYGVPVGLMVLSTAMFFLGSSLYIKLMAN 240
           FFNWYYATVG+SVTISV FMVYLQNAAGWVVGYGVPVGLM+ ST MFFLGSSLY+K+ AN
Sbjct: 181 FFNWYYATVGVSVTISVTFMVYLQNAAGWVVGYGVPVGLMLFSTIMFFLGSSLYVKIKAN 240

Query: 241 KSLLSSLAQVIVAAWRNRNLEFPPQASDKWFYHKGSKLVTPTPKLRFLNKACIIRNKETD 300
           KSLL SLAQVIVAAWRNR+L+ PPQ SDKWFYHKGSKLV+PTPKLRFLNKACIIRN+ETD
Sbjct: 241 KSLLGSLAQVIVAAWRNRHLDSPPQTSDKWFYHKGSKLVSPTPKLRFLNKACIIRNRETD 300

Query: 301 MDSNGMAKFPWRLSTIQRVEELKALIRLLPIWSTGIIISATINQFTFSALQANTMDRHIT 360
           +D+NGMA+FPW LSTIQRVEELKA+IR+LPIWSTGI+ISA I QFTF+ALQA+TMDRHIT
Sbjct: 301 VDANGMARFPWSLSTIQRVEELKAVIRVLPIWSTGIVISAAITQFTFAALQASTMDRHIT 360

Query: 361 PHFQFPAASFAVFTILTLTIWVAIYDQIIVPLLTKFTKKSNGLTLKQRMGIGLAISCLAS 420
           P FQFPAASFAVFTI+TLTIWVAIYDQIIV LL KFTK SNGL+LKQRMGIGLAISCLAS
Sbjct: 361 PRFQFPAASFAVFTIITLTIWVAIYDQIIVRLLAKFTKLSNGLSLKQRMGIGLAISCLAS 420

Query: 421 VVSAEIERKRRNRAIHEGLANVPEGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480
            VSAEIER RRNRAI EGL NVP GIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL
Sbjct: 421 AVSAEIERTRRNRAIDEGLGNVPNGIVKMSAMWLVPQHCLAGLAEALNAIGQIQFFYSQL 480

Query: 481 PRSMASIAVAMFSLGMGGGSLVAALIVSVVKKRTTKNGNVGWLSNNLNQGHYDYYYWVLS 540
           PRSMASIAVA+FSLGMGGGSL+ AL+VSV+KK T KNGNVGWLSNNLNQGHYDYYYWVLS
Sbjct: 481 PRSMASIAVALFSLGMGGGSLLGALVVSVIKKTTEKNGNVGWLSNNLNQGHYDYYYWVLS 540

Query: 541 LMGVANLLYYLICSWFYGDEEEGMEQSRVWDDKEAIEE 579
           +MGV N LYYLICSWFYGDE+EGME SRVWD+KEAIEE
Sbjct: 541 MMGVVNFLYYLICSWFYGDEKEGMEASRVWDEKEAIEE 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G52190.14.6e-16250.00Major facilitator superfamily protein [more]
AT3G16180.11.1e-15849.06Major facilitator superfamily protein [more]
AT1G68570.11.7e-11641.72Major facilitator superfamily protein [more]
AT1G69870.12.1e-11439.14nitrate transporter 1.7 [more]
AT1G27080.15.7e-10438.98nitrate transporter 1.6 [more]
Match NameE-valueIdentityDescription
Q9M8176.5e-16150.00Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1[more]
Q8LPL21.5e-15749.06Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2[more]
Q9SX202.4e-11541.72Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q8RX773.0e-11339.14Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
Q9LFX98.1e-10338.98Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_038879889.10.0e+00100.00protein NRT1/ PTR FAMILY 1.2-like [Benincasa hispida][more]
XP_008462682.13.9e-30490.92PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo] >KAA0046539.1 protei... [more]
KAE8652969.12.5e-30391.90hypothetical protein Csa_017739 [Cucumis sativus][more]
KAG6589921.13.1e-29388.21Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022987664.14.0e-29388.18protein NRT1/ PTR FAMILY 1.2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5A7TZ761.9e-30490.92Protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CHG51.9e-30490.92protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103500984 PE=3 S... [more]
A0A6J1JJI01.9e-29388.18protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111485150 PE... [more]
A0A6J1HA099.7e-29388.03protein NRT1/ PTR FAMILY 1.1-like OS=Cucurbita moschata OX=3662 GN=LOC111461844 ... [more]
A0A6J1CUG11.0e-28686.68protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111014453... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 2..566
e-value: 8.9E-158
score: 527.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 44..554
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 91..523
e-value: 2.4E-74
score: 250.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 16..564
NoneNo IPR availablePANTHERPTHR11654:SF150OS01G0761500 PROTEINcoord: 16..564
NoneNo IPR availableCDDcd17416MFS_NPF1_2coord: 26..557
e-value: 0.0
score: 544.168
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 80..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000959Bhi02M000959mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0006817 phosphate ion transport
biological_process GO:0016567 protein ubiquitination
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0022857 transmembrane transporter activity