Bhi02G000915 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000915
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNudix hydrolase 20
Locationchr2: 24923717 .. 24929035 (+)
RNA-Seq ExpressionBhi02G000915
SyntenyBhi02G000915
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGTATTTAAGCCTTGATTTTAAAAAGATGAGTTCCACGACGCCTATTCATCGATTTTTTGCTATCTGTTCTGGTAGTTAATGCTTATGGCTACCTCTCCTCCAATTCCACACATGGCCTGCAAATCTCTCGCTTCACACACATTTTGTATCTCTCACGCTTTCTCTTCAATCTGCAATGCTTCCATGTTTTCCCCCACGAGGTTCCTAACGCCACCTACATTTCGTTCGATGACCTTCGCCTTTCGTTCTGTATCGGTCACCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCCTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACAGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGTAGGTCTCTTTCTCCTTTTTCCTTATGTTTGAATGTTCTAAGTATGTATTTCGGCAGTAATTGTTTGCTTTAATTCGTTTTATTTCAAATTCAGCGCAGGAAAAGCTATAGATTGATTGAAATGCAGTTCCATATCTGTTTTGAATTGATTAAATTTTCTAACAGGATATGCAATCCGAATTTCTTCCGTTTGTCATCGATGACCTAATTCTTGGTTATGTGCACCACGGGTGAGGTTCCTACTGTTTTCTTTTTCCTACGCACATCGAAGATATCATCTCTTCTTGTAGTCCATGCTACTTGTTCGTACTGTTTGATCAAAGTAGGTTGTGTGTATTAGATTATATAATATTAAATTTACTTTCACCCATTAACTTAAACTTTTGGGTCCATTGGTGATTTAATACTGTATAACAGCACGTGGTACAGGAGGTCAAATGTTCAAACTCTTGTAATATTTACTCGAGACTGGGATCTATATAATATTCAATTTGTCTTCGTCTGGCTTAAGTTTTTGGGTTAATCGTTAATTTAATAGTGTGTTATGGATAAATTCAATGGATATGTAACTTATGCAGTTTCGGTAGACACCTGAAGAAGTATGCCAATGTGTTTACATTCCCCCGAGATGATTCTGTTGAATTTGGCGCTTGTTTAACATTGCATGAATCGCTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCTGGTACAAGGAATGAGGTACTGCTTGTAATTCCAATGTAAAGCAAGGATTAAAATATTTCTCAGTGCCTGGCTGTTTGGATCGTAACTGTGACATTGTATATAAAATATAATCGCCTTCCTCAGTTTAATGCGCAAATAAGCTGTTCCGTTCTAGAATAGGTACTTTAGAACAAGATAATGCTTGTTGTGGCTAACAGCATACAACCATGAACTCTGTCAGCTGTACCCTGTGACTTCATCCTTTGGTGGGCCGGTTTTTTTCTCGCTTGAACGTGCAGCAGCTCCTTATTTTGGAATAAAGGTGTGAATTTTCCCCTTCTACAGCACTATGGGAACTTCTATTTTCTTGTTCTATTTTTCCGTAATAAACTCTCAAAGAATTAGGCAATATGTCATATGTTTATACAAGTTTGGTTGTTAGGTCTAGATAAAGTTGAAACCTACTAATATATGATCGTCCTTTTCAATATAAATACAAAATCTCAAGTAATTTTTGCAGTACTATTCAATTCTGCCTACATTGCTGGAAGTAACTATTCCACTAATTGATTTCTCTAACTAACTTCTACCTGCCTTCTCTTGGTGCTTCTCTTAGCACAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGGAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACATTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGGTGTGTAATTGACAATATTTAACTGTTGTTTAGTTTTCTTGCATGTCACATCTCATGTTAAAAAAAAAAAAAGCGGTTTCCTCCCATGGTTTTAATTATATAAATACTTTAACATTTTCTCAAACTTGTGGACTTGAATATTTGTAAAAGATCTAAAAAGTGAAAATCAATAGTAATTGACGAGAAAATGACATTACATAGGTTTGAAGTTTGAACACAAGATCTTCGATTTCGATACTATGTTGAATCATCTATCAACAAAAGAATTTATTAATAAGTTATGGTAAATTTAATTATATTAATAATTTGACAATTGCTTTCTCCTTCCATTTTTCAAACACAGGCTTGCAAAGTTTTCCGATTGACTGAGGATAAAATTTCTGTTTTCTTATTCCCTTTTGTTTCTTATGCATGTAATTATATGAAAGACGCTTTCTAGAATTGCATTCCTTTATGTGAAATTGAGCCTGTTTAAGTTTCAGTCGACTCGTATTTGCTCTGTTTTGTTAAATGTAGTTAAGGCAGGCTACACTGATGATTTTTAGTTGTATTTGACTTTCTGTTTGAATGATAAGGTTGGATAGTTAATTACAAAATTAGCTAGGTTCTGATACTGACACATTTATATTTCTGGCTGAGACGGGTGAATTTTTATAATTTCCTACGAGCAGTAGAGAATCTGCCCAAACAGGGTTTGAGAAATGCATAAGAAAAGTTGGATACTGATGAAATGGGGAAAAAACAGGGGAGATTAGAAGTTAGAAATGTGCACTTGAATGCTGAACATCATGATTATTATCGTACCTTTATAACCAGCCCCTGACGATATGTTAGTGACAGCCTCAGGGCATCCCCTGCGGAGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCAAATAAGTAAATTCATAACAAAAATAATTATAATGTCACATTATTAGTCTAACCTTGTATTACATGGCTATGGTAGACGTAAACCTGGTGACTTTATAAAGCTTTTTTTTTTCAGGGCCATGCCAGTTGGTGCCGTTTCATATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATCTAAAGCTTCCTGAAAATTTTATACCGAAGAATCAAGGTTAGTTAACATGATCAATTTTCATTTACATGATCATTCAAAATTTAGATGAGGTCTCTTACATTGAATTTAATCTAGACCGCTCCAAATACCTCTAAATACAAATCTTAGTTAGAAATTCGAAACCACTAAATTTTGCTGACTAAACTTATGGCAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTATTATATACTATTTTGTCCATGGCTTTAAATTTGTTTCTCGACCATTACCTGCTTAAATTACGAGTTTTGTTGATCTTAGTGGTAAAAATTGAGAAAGGGTCGGAATTAGCCTCCTAAACTTTCTATCTTTCATAAATAACACCGTTTTAAATTTGTTGAAATAGTTTTTCTCGATCTATCTCATCTGAGATTGACCATCAGGATTTAGACGAAAAAAATTAACTTTTTCATCGTACCAAATGTTTTGGGCTTTTCCAGTTAAATCTCGAACAACCTCGAGATTCTATATATTTGGGCTTTCTTAATAAAATTTTGTGCAGGATTAACTCCGGGATTTTATATATTTCTTGAAATTTTACAATTTTTGTGCAAAATTTGATCTTTTCCAAAATTTAAAAACGACAGTGCAATTGCTTGAGATGAAAATTCATTTGCATAATTTGTAAATTCATTTGAAAATTTGGAAATCCAGATAATTGTGGAAATAGAAAATTAATTTTGAAAAATTACTCAAAATTTGATATTATGTTTGGTAAAGATAATTGAATTTGGAGAAATTTTGAAAAGATATAAGATTTGGTATAAGATTTGGAAAGATTATATAATATATTTGGAAAAATTAATAGAATCCTGGTGTTAATTTGAGACTTTGTCGAGTCTAAAATCCATGAGATGAAAAAATAATTTTTTTATGCAAATCTCGGTGGTCAATCTTGGTCAAGATGAAGTGAGAAAAATTATTTCAACAAATTTTCTAAATAGATGCTAGTTTTGAAAGTTTGAAATATTTTAAGAGCCATTTCTGAAAATTTTCCATAAAAAATCATGGATTTAAACGATTTGTAATAGGTACAAAATTTCTTAAATCAACAATAATTTCCAATGGAGTGTACATGAGTTGGGTTGGATTGGAGAGATTTTATGGACCAACCTAAAATTCGGGTTGGTCGGATTGACAAGCCGAATGACCCTAATAAGGTCTCCAACCTAATCCTTAAAATTTGGATTAGGTTGGGTTGTCGGGTTATTTATTCTTTTTCTTTTCTTTTTTATTACATTTTTTTATTAACTTACAATATTTAAATTTGTGTACAACACATACTGATAATTAAAATTTCATAAAACTGAAAGGTTAAATATCAAAATCCAAGATATTTATTACAAACTTCATACAAAAATCATAAAAATATATATAAATNCAAACTTCAAACAAACATCATAAAAATATATACAAACTATAATATTTATAAGTTATAATATATGTATTTTAATTATATTAGAAATTCGGGTTGGGTTGGGTCAACCCGAATTTTTAAAAGTAAACCAGAACCCAACCCAACTCGAAAAAAAACATAAAAAATTCAATCTAACTCAACAAAAAATTAAAACTAACCCAACCAACTCTTCTAATTTGGATTGATAGTCTGTGTTGTTCAGATTGTCGGGTCATTTGAACACTCCTATTGCTGAGGGCATTCTTAAGGGAGTGTATAGTTGCTTACAATTAATGTATTTTTCCTTTGAAAAATTATAGGTACATCATACCCGAAAGTTTAGGGTACTTGGAGCTCTTGCAAAGTTTGAAGAGTGGAACCTGCTTCTAAATAAAATCATCCGCCTTATTATAAATGTGAAAACTATGGTCATTCTCCAAGACGAGTATAGTTCAATTGACATTCGAGTGTATTAGAAACCACGAGTTTCATGGTTCGAATCTCCCTACTTCAATTGTTGTACTAAAAAAAACCTGTGGTCAGTCTTATTGAAAAATCGAGGATGGTTTATATTGTTTATTAGTTTGTCGGAATTTGCTCATTCAGGCTCTATCAATGTATTTGGGCATAGAATAAGGTTTTCATTTTTTTTAAAAAAAGTTATTTTTGTTATTCAACAATTACAAGGTAGAGATTGAACAATCGACATTAAAGAAGTATTTGATAGTTTTATCTATAAACTATGTTCGGATTAGTGGGATAAAGAATAAAATAGCATCA

mRNA sequence

AAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGTATTTAAGCCTTGATTTTAAAAAGATGAGTTCCACGACGCCTATTCATCGATTTTTTGCTATCTGTTCTGGTAGTTAATGCTTATGGCTACCTCTCCTCCAATTCCACACATGGCCTGCAAATCTCTCGCTTCACACACATTTTGTATCTCTCACGCTTTCTCTTCAATCTGCAATGCTTCCATGTTTTCCCCCACGAGGTTCCTAACGCCACCTACATTTCGTTCGATGACCTTCGCCTTTCGTTCTGTATCGGTCACCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCCTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACAGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAATTTCTTCCGTTTGTCATCGATGACCTAATTCTTGGTTATGTGCACCACGGTTTCGGTAGACACCTGAAGAAGTATGCCAATGTGTTTACATTCCCCCGAGATGATTCTGTTGAATTTGGCGCTTGTTTAACATTGCATGAATCGCTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCTGGTACAAGGAATGAGCTGTACCCTGTGACTTCATCCTTTGGTGGGCCGGTTTTTTTCTCGCTTGAACGTGCAGCAGCTCCTTATTTTGGAATAAAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGGAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACATTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTCAGGGCATCCCCTGCGGAGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCAAATAAGGCCATGCCAGTTGGTGCCGTTTCATATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATCTAAAGCTTCCTGAAAATTTTATACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTACATCATACCCGAAAGTTTAGGGTACTTGGAGCTCTTGCAAAGTTTGAAGAGTGGAACCTGCTTCTAAATAAAATCATCCGCCTTATTATAAATGTGAAAACTATGGTCATTCTCCAAGACGAGTATAGTTCAATTGACATTCGAGTGTATTAGAAACCACGAGTTTCATGGTTCGAATCTCCCTACTTCAATTGTTGTACTAAAAAAAACCTGTGGTCAGTCTTATTGAAAAATCGAGGATGGTTTATATTGTTTATTAGTTTGTCGGAATTTGCTCATTCAGGCTCTATCAATGTATTTGGGCATAGAATAAGGTTTTCATTTTTTTTAAAAAAAGTTATTTTTGTTATTCAACAATTACAAGGTAGAGATTGAACAATCGACATTAAAGAAGTATTTGATAGTTTTATCTATAAACTATGTTCGGATTAGTGGGATAAAGAATAAAATAGCATCA

Coding sequence (CDS)

ATGCTTATGGCTACCTCTCCTCCAATTCCACACATGGCCTGCAAATCTCTCGCTTCACACACATTTTGTATCTCTCACGCTTTCTCTTCAATCTGCAATGCTTCCATGTTTTCCCCCACGAGGTTCCTAACGCCACCTACATTTCGTTCGATGACCTTCGCCTTTCGTTCTGTATCGGTCACCAGCAGCAGTTTCACTTGGGAAGACGCTGTCCACATTTCCTTGCCAGAAAGCTTTCAGGATGATCCCTCAGATCTTACAGGCTATTTCGAGAAGGTCAAGCTCTGCAATCGCGGATCAGATATGCAATCCGAATTTCTTCCGTTTGTCATCGATGACCTAATTCTTGGTTATGTGCACCACGGTTTCGGTAGACACCTGAAGAAGTATGCCAATGTGTTTACATTCCCCCGAGATGATTCTGTTGAATTTGGCGCTTGTTTAACATTGCATGAATCGCTAAAGACACCGGAAGATAGGACGCATGCAGTTGGAGATGTAGTCAAATGCTTGGGTGAAGAAGTTATTCCTGGTACAAGGAATGAGCTGTACCCTGTGACTTCATCCTTTGGTGGGCCGGTTTTTTTCTCGCTTGAACGTGCAGCAGCTCCTTATTTTGGAATAAAGGTTTATGGAGTTCAGATGAATGGTTATGTTGAAAAGGATGGGAAGAAATTTTTGTGGATAGCAAAAAGAAGTCAAATGAAGCAAACATTTCCTGGAATGCTGGATGAATTAGTAGCTGGAGGGCTGCCTCAGGGCATCCCCTGCGGAGAGAATCTGATGAAGGAATGTGAGGAGGAAGCAGGCATACCAAGATCAATATCAAATAAGGCCATGCCAGTTGGTGCCGTTTCATATTCAGACATCAAGGATTATAGTTACAAGAGAGATGTTCTATTTTGTTACGATCTAAAGCTTCCTGAAAATTTTATACCGAAGAATCAAGATGGGGAGGTTGAAGGATTCAGGTTGTTGCCTGTGACAAATGTTGCAAATGTGATTCGGAGGACACAGTTATTCAAGCATAATTGTGCTCTTGTCATCATTGATTTCCTATTTCGACATGGGTACATCATACCCGAAAGTTTAGGGTACTTGGAGCTCTTGCAAAGTTTGAAGAGTGGAACCTGCTTCTAA

Protein sequence

MLMATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFRSMTFAFRSVSVTSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGYLELLQSLKSGTCF
Homology
BLAST of Bhi02G000915 vs. TAIR 10
Match: AT5G19460.1 (nudix hydrolase homolog 20 )

HSP 1 Score: 415.2 bits (1066), Expect = 5.5e-116
Identity = 198/320 (61.88%), Postives = 247/320 (77.19%), Query Frame = 0

Query: 62  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 121
           SSSFTW+D +     E    + SDLTG+FEK+  CNRGS+   EF+PFVI++ I+GY+H 
Sbjct: 57  SSSFTWDDVIETGRAEY---NSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHK 116

Query: 122 GFGRHLKKYANVFTFPRDDSV--EFGACLTLHESLKTPEDRTHAVGDVVKCLGEE-VIPG 181
            F  +L+++ ++FTF ++ S        +TL+  L+ PEDRT AV DV+K LG++ +IPG
Sbjct: 117 RFTEYLREFHDIFTFSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPG 176

Query: 182 TRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241
            RNELYPV  SF  PVFFSLERAAAPYFGIK YGV MNGYVE+DG+K LWI KRS  K T
Sbjct: 177 IRNELYPVKPSFNAPVFFSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKST 236

Query: 242 FPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRD 301
           +PGMLD LVAGGLP GI CG NL+KECEEEAGI R+I+++A+ VGAVSY DI  Y +KRD
Sbjct: 237 YPGMLDHLVAGGLPHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRD 296

Query: 302 VLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 361
           VLFCYDL+LPE+F+PKNQDGEVE F+L+PV  VA+VI++T  FK NC+LVIIDFLFRHG+
Sbjct: 297 VLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVIIDFLFRHGF 356

Query: 362 IIPESLGYLELLQSLKSGTC 379
           I PES GYL+L Q L++  C
Sbjct: 357 IRPESSGYLDLYQRLRNRDC 373

BLAST of Bhi02G000915 vs. TAIR 10
Match: AT5G19470.1 (nudix hydrolase homolog 24 )

HSP 1 Score: 388.3 bits (996), Expect = 7.2e-108
Identity = 194/370 (52.43%), Postives = 254/370 (68.65%), Query Frame = 0

Query: 24  ISHAFSSIC--NASMFSPTRFL------------TPPTFRSMTFAFRSVSVTSSSFTWED 83
           ++ AF S+C    S+FS    +            +PP   S   +  +V + S SFTW D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWND 60

Query: 84  AVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRHLKK 143
               S  E    + SDLTG+ EKV  CNRG +   EF+PFVI++ I+GY+H GF ++L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 144 YANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEE-VIPGTRNELYPVTS 203
           + ++FTF      ++G  +TL+  L  PE+RT AV  V+K LG + +IPG RNELYPV  
Sbjct: 121 FNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 204 SFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVA 263
           SF  P FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K T+PG LD LVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 264 GGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLKLP 323
           GGLP GI   ENL+KECEEEAGI + ++++A+ VG VSY DI  Y + RDVLFCYDL+LP
Sbjct: 241 GGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 324 ENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGYLE 379
           ++F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRHG I PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 360

BLAST of Bhi02G000915 vs. TAIR 10
Match: AT5G19470.2 (nudix hydrolase homolog 24 )

HSP 1 Score: 374.4 bits (960), Expect = 1.1e-103
Identity = 190/370 (51.35%), Postives = 250/370 (67.57%), Query Frame = 0

Query: 24  ISHAFSSIC--NASMFSPTRFL------------TPPTFRSMTFAFRSVSVTSSSFTWED 83
           ++ AF S+C    S+FS    +            +PP   S   +  +V + S SFTW D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWND 60

Query: 84  AVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRHLKK 143
               S  E    + SDLTG+ EKV  CNRG +   EF+PFVI++ I+GY+H GF ++L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 144 YANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEE-VIPGTRNELYPVTS 203
           + ++FTF      ++G  +TL+  L  PE+RT AV  V+K LG + +IPG RNELYPV  
Sbjct: 121 FNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 204 SFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVA 263
           SF  P FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K T+PG LD LVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 264 GGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLKLP 323
           GGL        NL+KECEEEAGI + ++++A+ VG VSY DI  Y + RDVLFCYDL+LP
Sbjct: 241 GGL--------NLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 324 ENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGYLE 379
           ++F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRHG I PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 356

BLAST of Bhi02G000915 vs. ExPASy Swiss-Prot
Match: Q8VXZ0 (Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.8e-115
Identity = 198/320 (61.88%), Postives = 247/320 (77.19%), Query Frame = 0

Query: 62  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 121
           SSSFTW+D +     E    + SDLTG+FEK+  CNRGS+   EF+PFVI++ I+GY+H 
Sbjct: 57  SSSFTWDDVIETGRAEY---NSSDLTGFFEKINRCNRGSEKLGEFIPFVIEEQIVGYIHK 116

Query: 122 GFGRHLKKYANVFTFPRDDSV--EFGACLTLHESLKTPEDRTHAVGDVVKCLGEE-VIPG 181
            F  +L+++ ++FTF ++ S        +TL+  L+ PEDRT AV DV+K LG++ +IPG
Sbjct: 117 RFTEYLREFHDIFTFSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKGIIPG 176

Query: 182 TRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQT 241
            RNELYPV  SF  PVFFSLERAAAPYFGIK YGV MNGYVE+DG+K LWI KRS  K T
Sbjct: 177 IRNELYPVKPSFNAPVFFSLERAAAPYFGIKGYGVHMNGYVERDGQKLLWIGKRSLSKST 236

Query: 242 FPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRD 301
           +PGMLD LVAGGLP GI CG NL+KECEEEAGI R+I+++A+ VGAVSY DI  Y +KRD
Sbjct: 237 YPGMLDHLVAGGLPHGISCGGNLVKECEEEAGISRAIADRAIAVGAVSYLDIDQYCFKRD 296

Query: 302 VLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGY 361
           VLFCYDL+LPE+F+PKNQDGEVE F+L+PV  VA+VI++T  FK NC+LVIIDFLFRHG+
Sbjct: 297 VLFCYDLELPEDFVPKNQDGEVESFKLIPVAQVASVIKKTSFFKANCSLVIIDFLFRHGF 356

Query: 362 IIPESLGYLELLQSLKSGTC 379
           I PES GYL+L Q L++  C
Sbjct: 357 IRPESSGYLDLYQRLRNRDC 373

BLAST of Bhi02G000915 vs. ExPASy Swiss-Prot
Match: P0C026 (Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.0e-106
Identity = 194/370 (52.43%), Postives = 254/370 (68.65%), Query Frame = 0

Query: 24  ISHAFSSIC--NASMFSPTRFL------------TPPTFRSMTFAFRSVSVTSSSFTWED 83
           ++ AF S+C    S+FS    +            +PP   S   +  +V + S SFTW D
Sbjct: 1   MASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPL-SPSFTWND 60

Query: 84  AVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRHLKK 143
               S  E    + SDLTG+ EKV  CNRG +   EF+PFVI++ I+GY+H GF ++L+ 
Sbjct: 61  VFENSRKEYVPQNSSDLTGFLEKVDRCNRGLEKLGEFIPFVIEEQIVGYIHKGFTKYLRD 120

Query: 144 YANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEE-VIPGTRNELYPVTS 203
           + ++FTF      ++G  +TL+  L  PE+RT AV  V+K LG + +IPG RNELYPV  
Sbjct: 121 FNDIFTFS-----QYGGHVTLNMMLDKPEERTRAVAHVIKILGNKGIIPGIRNELYPVKP 180

Query: 204 SFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELVA 263
           SF  P FFS+ERAAAPYFG+K Y + +NGYVE+DG+KFLWI KRS  K T+PG LD LVA
Sbjct: 181 SFNAPAFFSIERAAAPYFGLKGYAIHVNGYVERDGQKFLWIGKRSLAKSTYPGKLDHLVA 240

Query: 264 GGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLKLP 323
           GGLP GI   ENL+KECEEEAGI + ++++A+ VG VSY DI  Y + RDVLFCYDL+LP
Sbjct: 241 GGLPHGISVCENLVKECEEEAGISKVLADRAIAVGVVSYMDIDRYCFTRDVLFCYDLELP 300

Query: 324 ENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGYLE 379
           ++F+P NQDGEV+ FRL+PV  VANV+R+T  FK +C+LVIIDFLFRHG I PES GYL+
Sbjct: 301 QDFVPTNQDGEVDSFRLIPVAQVANVVRKTSFFKDSCSLVIIDFLFRHGLIRPESPGYLD 360

BLAST of Bhi02G000915 vs. ExPASy Swiss-Prot
Match: P47173 (Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJR142W PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.3e-33
Identity = 91/212 (42.92%), Postives = 121/212 (57.08%), Query Frame = 0

Query: 176 IPGTRNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYV--EKDGKKFLWIAKRS 235
           + G RNE Y V        +  +ERA A   GI  YG+ +NGYV   K  K   W+ +RS
Sbjct: 123 VKGWRNEKYAVW--VNKKPYVLVERAVAGVLGIITYGIHINGYVLDPKSKKVQFWVPRRS 182

Query: 236 QMKQTFPGMLDELVAGGLPQGIPCG--ENLMKECEEEAGIPRS-ISNKAMPVGAVSY--- 295
           + KQT+P MLD ++AGGL  G P G  E ++KE  EEA + +S I +     G+VSY   
Sbjct: 183 KTKQTWPLMLDNIIAGGL--GYPYGIYETVLKESMEEANLEKSVIEDNIKATGSVSYLYF 242

Query: 296 -SDIKDYSYKR-------DVLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQ 355
             DI    + +       +V + YDLKL E+ IPK  DGEVE F L  +    N +R+ +
Sbjct: 243 TGDISVTKFNKESDFIVGEVQYVYDLKLSEDIIPKPNDGEVESFNLFSLQETINALRKKE 302

Query: 356 LFKHNCALVIIDFLFRHGYIIPES-LGYLELL 371
            FK NCALV++DFL RHGYI PE+   YLEL+
Sbjct: 303 -FKPNCALVMVDFLIRHGYITPENEPNYLELV 329

BLAST of Bhi02G000915 vs. ExPASy Swiss-Prot
Match: P41888 (Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tnr3 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.8e-26
Identity = 75/197 (38.07%), Postives = 108/197 (54.82%), Query Frame = 0

Query: 180 RNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYV--EKDGKKFLWIAKRSQMKQ 239
           RNELY V      PV  ++ER     FG    GV    Y+   K+    +W+ +RS  KQ
Sbjct: 105 RNELYTVYGKSKKPV-LAVERGGFWLFGFLSTGVHCTMYIPATKEHPLRIWVPRRSPTKQ 164

Query: 240 TFPGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDI-KDYSYK 299
           T+P  LD  VAGG+  G      ++KE  EEA +  S  N  +P G VSY  + K +  +
Sbjct: 165 TWPNYLDNSVAGGIAHGDSVIGTMIKEFSEEANLDVSSMN-LIPCGTVSYIKMEKRHWIQ 224

Query: 300 RDVLFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRH 359
            ++ + +DL + +  IP+  DGEV GF LLP+  V + +   + FK NCALV++DFL RH
Sbjct: 225 PELQYVFDLPVDDLVIPRINDGEVAGFSLLPLNQVLHEL-ELKSFKPNCALVLLDFLIRH 284

Query: 360 GYIIPESLGYLELLQSL 374
           G I P+   YL+ L+ +
Sbjct: 285 GIITPQHPQYLQTLERI 298

BLAST of Bhi02G000915 vs. NCBI nr
Match: XP_038878620.1 (nudix hydrolase 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 782.7 bits (2020), Expect = 1.4e-222
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 1   MLMATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFRSMTFAFRSVSV 60
           MLMATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFRSMTFAFRSVSV
Sbjct: 1   MLMATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFRSMTFAFRSVSV 60

Query: 61  TSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVH 120
           TSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVH
Sbjct: 61  TSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVH 120

Query: 121 HGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTR 180
           HGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTR
Sbjct: 121 HGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTR 180

Query: 181 NELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFP 240
           NELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFP
Sbjct: 181 NELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFP 240

Query: 241 GMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVL 300
           GMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVL
Sbjct: 241 GMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVL 300

Query: 301 FCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYII 360
           FCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYII
Sbjct: 301 FCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYII 360

Query: 361 PESLGYLELLQSLKSGTCF 380
           PESLGYLELLQSLKSGTCF
Sbjct: 361 PESLGYLELLQSLKSGTCF 379

BLAST of Bhi02G000915 vs. NCBI nr
Match: XP_023530783.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023530784.1 nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675.2 bits (1741), Expect = 3.2e-190
Identity = 334/378 (88.36%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPT-FRSMTFAFRSVSVT 62
           M T P I  MACKSLASH F  S   SSI NASMFS   + T  T  RS TFA RSVSV 
Sbjct: 1   MVTCPRIQPMACKSLASHRFRNSLPISSISNASMFSRATYPTASTVHRSKTFACRSVSVA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+IPG RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+G+ CGENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           ESLGYLELLQSLKSGTCF
Sbjct: 361 ESLGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. NCBI nr
Match: XP_022933802.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 4.2e-190
Identity = 334/378 (88.36%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTF-RSMTFAFRSVSVT 62
           MAT P I  MACKSLASH F  S   SSI NASMFS  R+ T  T  RS TFA RSVSV 
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTASTLHRSKTFACRSVSVA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+IPG RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAA YFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+G+ CGENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           ES GYLELLQSLKSGTCF
Sbjct: 361 ESSGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. NCBI nr
Match: XP_023002396.1 (nudix hydrolase 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 674.5 bits (1739), Expect = 5.4e-190
Identity = 333/378 (88.10%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPT-FRSMTFAFRSVSVT 62
           MAT P I  MACKSLASH F  S   SSI NASMFS   + T  T  RS TFA RSVS+ 
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTASTVHRSKTFACRSVSIA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+IPG RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGVRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+GI CGENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGISCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII+FLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIEFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           E LGYLELLQSLKSGTCF
Sbjct: 361 ECLGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. NCBI nr
Match: KAG6587948.1 (Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.0 bits (1730), Expect = 6.0e-189
Identity = 333/378 (88.10%), Postives = 345/378 (91.27%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPT-FRSMTFAFRSVSVT 62
           MAT P I  MACKSLASH F  S   SSI NASMFS   + T  T  RS TFA RSVSV 
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSIFNASMFSRATYPTASTVHRSKTFACRSVSVA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+I G RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIQGIRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+G+ C ENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCEENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           ESLGYLELLQSLKSGTCF
Sbjct: 361 ESLGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. ExPASy TrEMBL
Match: A0A6J1F0U6 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441109 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.0e-190
Identity = 334/378 (88.36%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTF-RSMTFAFRSVSVT 62
           MAT P I  MACKSLASH F  S   SSI NASMFS  R+ T  T  RS TFA RSVSV 
Sbjct: 1   MATCPRIQPMACKSLASHRFRNSSPISSISNASMFSRARYPTASTLHRSKTFACRSVSVA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+IPG RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGIRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAA YFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAATYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+G+ CGENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGVSCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           ES GYLELLQSLKSGTCF
Sbjct: 361 ESSGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. ExPASy TrEMBL
Match: A0A6J1KL70 (nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496248 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.6e-190
Identity = 333/378 (88.10%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 3   MATSPPIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPT-FRSMTFAFRSVSVT 62
           MAT P I  MACKSLASH F  S   SSI NASMFS   + T  T  RS TFA RSVS+ 
Sbjct: 1   MATCPRIQPMACKSLASHPFRNSSPISSISNASMFSRATYPTASTVHRSKTFACRSVSIA 60

Query: 63  SSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHH 122
           SS+FTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDD I+G+VHH
Sbjct: 61  SSNFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDQIVGHVHH 120

Query: 123 GFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRN 182
           GF  HLKKYANVFTFPRDDSV  GACL+LHESLKTPEDRTHAVG+VVKCLGEE+IPG RN
Sbjct: 121 GFAYHLKKYANVFTFPRDDSVRVGACLSLHESLKTPEDRTHAVGNVVKCLGEEIIPGVRN 180

Query: 183 ELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 242
           E+YPVTSSFG PVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG
Sbjct: 181 EMYPVTSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPG 240

Query: 243 MLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLF 302
           MLDELVAGGLP+GI CGENLMKECEEEAGIPRSISNKA+PVGAVSYSDIKDYSYKRDVLF
Sbjct: 241 MLDELVAGGLPEGISCGENLMKECEEEAGIPRSISNKAIPVGAVSYSDIKDYSYKRDVLF 300

Query: 303 CYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIP 362
           CYDLKLPE+FIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVII+FLFRHGYI P
Sbjct: 301 CYDLKLPESFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIEFLFRHGYIKP 360

Query: 363 ESLGYLELLQSLKSGTCF 380
           E LGYLELLQSLKSGTCF
Sbjct: 361 ECLGYLELLQSLKSGTCF 378

BLAST of Bhi02G000915 vs. ExPASy TrEMBL
Match: A0A6J1CUZ7 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014513 PE=4 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.2e-184
Identity = 323/373 (86.60%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 12  MACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTF-----RSMTFAFRSVSVTSSSFT 71
           MACKSLA H FC   A S IC+ASMF  TRF T  TF     RS+TFA  SVSV SSSFT
Sbjct: 1   MACKSLAPHRFCNFPAISFICDASMFLRTRFPTTSTFRPTVHRSITFASGSVSVASSSFT 60

Query: 72  WEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRH 131
           WEDA+HISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVID+ I+GYVHHGF  H
Sbjct: 61  WEDALHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDNQIIGYVHHGFAEH 120

Query: 132 LKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRNELYPV 191
           LKKYANVFTFP+DDSV+ GA LTL+ESL TPEDRT  VGDVVKCLGEEVIPGTRNELYPV
Sbjct: 121 LKKYANVFTFPQDDSVQCGAYLTLNESLTTPEDRTRGVGDVVKCLGEEVIPGTRNELYPV 180

Query: 192 TSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDEL 251
           TSSFG PVFFSLERAAAPYFGIKVYGVQMNGYVE+DG+KFLWIAKRSQMKQTFPGMLDEL
Sbjct: 181 TSSFGAPVFFSLERAAAPYFGIKVYGVQMNGYVERDGQKFLWIAKRSQMKQTFPGMLDEL 240

Query: 252 VAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLK 311
           VAGGLPQG+PCGENL KECEEEAGIPRSISNKA+ VGAVSYSDIKDYSYKRDVLFCYDLK
Sbjct: 241 VAGGLPQGVPCGENLRKECEEEAGIPRSISNKAISVGAVSYSDIKDYSYKRDVLFCYDLK 300

Query: 312 LPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGY 371
           LPE+F+PKNQDGEVEGFRLLPV NVANVIRRTQLFK NC+LVIIDFLFRHG I PESLGY
Sbjct: 301 LPESFVPKNQDGEVEGFRLLPVENVANVIRRTQLFKPNCSLVIIDFLFRHGCIKPESLGY 360

Query: 372 LELLQSLKSGTCF 380
           LELLQSLKSGTCF
Sbjct: 361 LELLQSLKSGTCF 373

BLAST of Bhi02G000915 vs. ExPASy TrEMBL
Match: A0A6J1CSY3 (nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111014470 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.8e-170
Identity = 304/380 (80.00%), Postives = 328/380 (86.32%), Query Frame = 0

Query: 8   PIPHMACKSLASHT--FCISHAFSSICNASMFSPTRFLTPPTF------RSMTFAFRSVS 67
           PI  MACKSLASH+  F      SS CNAS  S T +    TF      RS++ A RSVS
Sbjct: 13  PIQFMACKSLASHSHRFRNFSTISSNCNASTISRTTYPMASTFRPTVVHRSISVASRSVS 72

Query: 68  VTSSSFTWEDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYV 127
           V +SSFTW+D + ISLPE +QDDPSDLTGYFEKVKLCNRGSDMQSEFLPFV+DD I+GYV
Sbjct: 73  V-ASSFTWDDVLRISLPEIYQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVVDDQIVGYV 132

Query: 128 HHGFGRHLKKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGT 187
           HHGF  HLK+YANVFTF  DDSV+F A LTLHESLKTPEDRT AVGDVVKCLGEE+IPGT
Sbjct: 133 HHGFAEHLKQYANVFTFSGDDSVQFRAYLTLHESLKTPEDRTQAVGDVVKCLGEEIIPGT 192

Query: 188 RNELYPVTSSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTF 247
           RNELYPVTSSFG P FFSLERAAAPYFGIKVYGV MNGYVEK+GKKFLW+AKRSQ KQTF
Sbjct: 193 RNELYPVTSSFGAPNFFSLERAAAPYFGIKVYGVHMNGYVEKEGKKFLWVAKRSQTKQTF 252

Query: 248 PGMLDELVAGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDV 307
           PGMLD LVAGGLPQGIPC ENLMKECEEEA IPRS+SN+A+PVGAVSYSDIK ++YKRDV
Sbjct: 253 PGMLDHLVAGGLPQGIPCVENLMKECEEEACIPRSLSNEAIPVGAVSYSDIKGHTYKRDV 312

Query: 308 LFCYDLKLPENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYI 367
            FCYDLKLPE+FIP+NQDGEVEGF LLPVTNVANVIRRTQLFK NC+LVIIDFLFRHGYI
Sbjct: 313 QFCYDLKLPESFIPENQDGEVEGFMLLPVTNVANVIRRTQLFKPNCSLVIIDFLFRHGYI 372

Query: 368 IPESLGYLELLQSLKSGTCF 380
            PES GYLELLQSL+SGTCF
Sbjct: 373 YPESSGYLELLQSLRSGTCF 391

BLAST of Bhi02G000915 vs. ExPASy TrEMBL
Match: A0A6J1JI14 (LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485288 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.8e-168
Identity = 295/372 (79.30%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 8   PIPHMACKSLASHTFCISHAFSSICNASMFSPTRFLTPPTFRSMTFAFRSVSVTSSSFTW 67
           PI  MACKSL +H F      SS+ NAS  S T +    TFR      RS+SV + +F+W
Sbjct: 13  PIQFMACKSLVAHRFHNFSIVSSLYNASKISRTTYPMLSTFRPA--VTRSISVAAGNFSW 72

Query: 68  EDAVHISLPESFQDDPSDLTGYFEKVKLCNRGSDMQSEFLPFVIDDLILGYVHHGFGRHL 127
           +D VHISLP SFQDDPSDLTGYFEKVKLCNRGSD QSEFLPFVIDDLI+GYVHHGF +HL
Sbjct: 73  DDVVHISLPSSFQDDPSDLTGYFEKVKLCNRGSDTQSEFLPFVIDDLIVGYVHHGFAKHL 132

Query: 128 KKYANVFTFPRDDSVEFGACLTLHESLKTPEDRTHAVGDVVKCLGEEVIPGTRNELYPVT 187
           K+Y NVF FPRDD  +FGA LTLHESLKTPEDRTHAVGDVVKCLG+EVIPGTRNELYPVT
Sbjct: 133 KQYPNVFIFPRDDCGQFGAYLTLHESLKTPEDRTHAVGDVVKCLGKEVIPGTRNELYPVT 192

Query: 188 SSFGGPVFFSLERAAAPYFGIKVYGVQMNGYVEKDGKKFLWIAKRSQMKQTFPGMLDELV 247
           SSFG   FFSLERAAAPYFGIKVYGV MNGYVEK+G+KFLW+AKRSQ K TFPGMLD LV
Sbjct: 193 SSFGASNFFSLERAAAPYFGIKVYGVHMNGYVEKEGEKFLWVAKRSQTKPTFPGMLDHLV 252

Query: 248 AGGLPQGIPCGENLMKECEEEAGIPRSISNKAMPVGAVSYSDIKDYSYKRDVLFCYDLKL 307
           AGGLPQGIPCGEN+MKECEEEAGIPRSIS  A+PVGA+SY+DIK +SYKRDV FCYDLKL
Sbjct: 253 AGGLPQGIPCGENVMKECEEEAGIPRSISKGAIPVGAISYTDIKGFSYKRDVQFCYDLKL 312

Query: 308 PENFIPKNQDGEVEGFRLLPVTNVANVIRRTQLFKHNCALVIIDFLFRHGYIIPESLGYL 367
           PE+FIP+NQDGEVEGF LL VTNVANVIRRTQLFK NC LVIIDFLFRHGYI PES GYL
Sbjct: 313 PESFIPENQDGEVEGFMLLLVTNVANVIRRTQLFKPNCTLVIIDFLFRHGYINPESSGYL 372

Query: 368 ELLQSLKSGTCF 380
           ELLQSL+SGTCF
Sbjct: 373 ELLQSLRSGTCF 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G19460.15.5e-11661.88nudix hydrolase homolog 20 [more]
AT5G19470.17.2e-10852.43nudix hydrolase homolog 24 [more]
AT5G19470.21.1e-10351.35nudix hydrolase homolog 24 [more]
Match NameE-valueIdentityDescription
Q8VXZ07.8e-11561.88Nudix hydrolase 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT20 PE=2... [more]
P0C0261.0e-10652.43Nudix hydrolase 24, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT24 PE=2... [more]
P471733.3e-3342.92Uncharacterized protein YJR142W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
P418881.8e-2638.07Thiamine pyrophosphokinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843... [more]
Match NameE-valueIdentityDescription
XP_038878620.11.4e-222100.00nudix hydrolase 20, chloroplastic-like [Benincasa hispida][more]
XP_023530783.13.2e-19088.36nudix hydrolase 20, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02353078... [more]
XP_022933802.14.2e-19088.36nudix hydrolase 20, chloroplastic-like [Cucurbita moschata][more]
XP_023002396.15.4e-19088.10nudix hydrolase 20, chloroplastic-like [Cucurbita maxima][more]
KAG6587948.16.0e-18988.10Nudix hydrolase 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1F0U62.0e-19088.36nudix hydrolase 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1KL702.6e-19088.10nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114962... [more]
A0A6J1CUZ78.2e-18486.60nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1CSY38.8e-17080.00nudix hydrolase 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1JI142.8e-16879.30LOW QUALITY PROTEIN: nudix hydrolase 20, chloroplastic-like OS=Cucurbita maxima ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 222..331
e-value: 6.3E-7
score: 29.5
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 210..351
score: 9.916633
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 195..375
e-value: 1.6E-65
score: 222.2
NoneNo IPR availablePANTHERPTHR13622:SF10SI:DKEY-6N6.2coord: 63..376
NoneNo IPR availablePANTHERPTHR13622THIAMIN PYROPHOSPHOKINASEcoord: 63..376
NoneNo IPR availableCDDcd03676Nudix_hydrolase_3coord: 178..357
e-value: 7.08573E-79
score: 238.318
IPR031804Domain of unknown function DUF4743PFAMPF15916DUF4743coord: 89..209
e-value: 1.7E-32
score: 111.9
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 186..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000915Bhi02M000915mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0044715 8-oxo-dGDP phosphatase activity