Bhi02G000876 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000876
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCytochrome P450
Locationchr2: 23509117 .. 23510269 (-)
RNA-Seq ExpressionBhi02G000876
SyntenyBhi02G000876
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATTGAATCAGTGTTATCGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATGATTTTAAGTGCAAAAATGGTGTTGGTTTTATGGTGGAAACCACGAAGAATTGAAAAACACTTTGATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAAGTAGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCCCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGTACGTTTAATTTTTTTCATTATATTTCTAATTCAAGATCATTCCCATCCAATTCAAAAAGTCTGTTTGAATTGACTTTCTAGGTTCAACCTTTCTAATTTGGTTTGGACCAATTGTCCGAGTCGCCGTCTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCCCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTTAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAGGGTCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTCCATATGGATAATCTCAAAGTAAATTTCTGACTCGGTTTTTATTTTTTTATTTTTTAATATATATATCTTAACTAGTTGAGGTAAATTTATAATTGTTGTTAGTTGTTGATTCCATTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTACCGACTCTGACGAGGTCGAAATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTATGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCCCAAGCTTTTCACAAGGTCTTCATTCCTGGCTATAGGTAAAATTATAATTTCCTTTTTTTTTTTTAATTTCTTTCTAATTTTGGGTTTTTTTTTTTAAAAAAAAATTAATATAAATGTTTGTTTTTCAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGTCGAAAATGGAATTCAGAAAAGAATTGGGGAATGGATGA

mRNA sequence

ATGAAGATTGAATCAGTGTTATCGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATGATTTTAAGTGCAAAAATGGTGTTGGTTTTATGGTGGAAACCACGAAGAATTGAAAAACACTTTGATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAAGTAGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCCCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGTTCAACCTTTCTAATTTGGTTTGGACCAATTGTCCGAGTCGCCGTCTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCCCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTTAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAGGGTCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTCCATATGGATAATCTCAAATTGTTGATTCCATTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTACCGACTCTGACGAGGTCGAAATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTATGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCCCAAGCTTTTCACAAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGTCGAAAATGGAATTCAGAAAAGAATTGGGGAATGGATGA

Coding sequence (CDS)

ATGAAGATTGAATCAGTGTTATCGAAGGTGCCACCGGTATGGGCGGCGGCGGTGACGGCGGCGGTGGTGATGATTTTAAGTGCAAAAATGGTGTTGGTTTTATGGTGGAAACCACGAAGAATTGAAAAACACTTTGATAGACAAGGAATTCGAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTTAAAGAAGTAGTTTCTATGATGCTTGCTGCTTCTTCTCATCCAATGCCCAATTTCTCCCATAATATTCTCCCTCGTGTCCTCTCTTTTTACCATCATTGGAAGAAAATTTACGGTTCAACCTTTCTAATTTGGTTTGGACCAATTGTCCGAGTCGCCGTCTCCGACCCGGACATGATCCGAGAAATCTTCACCACTAATTCCCACTTCTACGAGAAGAATGAAGCTCATCCTCTTGTTAAACAGCTCGAAGGCGATGGCCTCCTCACTCTCAAGGGTCAAAAATGGGCTCTCCATCGCAAAATCATCTCCCCCACTTTCCATATGGATAATCTCAAATTGTTGATTCCATTGGTGGAGGAAAGTGTGGTGGAAATGGTGGAGAAATGGTCGTCTATGGCGGAGAGTACCGACTCTGACGAGGTCGAAATCGATGTTTTCGAGTGCTTTCAAACCCTAACGGAAGATGTTATTACAAAAACAGCATTTGGTAGTAGCTATGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCCCAAGCTTTTCACAAGATTTCTACCGACAAGAACGAATATAAATTCGTGGAAATTAGACAAGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGTCGAAAATGGAATTCAGAAAAGAATTGGGGAATGGATGA

Protein sequence

MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHKISTDKNEYKFVEIRQGNKEIIDEINRGSKMEFRKELGNG
Homology
BLAST of Bhi02G000876 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.6e-94
Identity = 167/252 (66.27%), Postives = 211/252 (83.73%), Query Frame = 0

Query: 1   MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG 60
           M+ ES    +P V   +V  ++V++   K + +LWW+PR+IE+HF +QGIRGPPY FFIG
Sbjct: 1   MEEESSSWFIPKVLVLSVILSLVIV---KGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIG 60

Query: 61  NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           N KE+V MML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+
Sbjct: 61  NVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDL 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV 180
           IREIF + S FYEKNEAHPLVKQLEGDGLL+LKG+KWA HRKIISPTFHM+NLKLL+P+V
Sbjct: 121 IREIF-SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVV 180

Query: 181 EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
            +SV +MV+KWS   + +++ EVE+DV+E FQ LTEDVI++TAFGSSYEDG+A+F+LQ Q
Sbjct: 181 LKSVTDMVDKWSD--KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQ 240

Query: 241 QMILASQAFHKI 253
           QM+L ++AF K+
Sbjct: 241 QMLLCAEAFQKV 245

BLAST of Bhi02G000876 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 169.5 bits (428), Expect = 4.0e-42
Identity = 104/281 (37.01%), Postives = 160/281 (56.94%), Query Frame = 0

Query: 13  VWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAA 72
           V A  V  AVV++ + ++V  +W KP+ +E    RQG+ G PY   +G+ K  V MM+ A
Sbjct: 6   VAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEA 65

Query: 73  SSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFY 132
            S P+ N + +I PR+L         +G TF IW GP+  + +++P+ I+E+F   + F 
Sbjct: 66  RSKPI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDF- 125

Query: 133 EKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWS 192
           EK    PL++ L G GL + KG KWA HR+II+P FH++ +K +IP       E+V +W 
Sbjct: 126 EKASTFPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWE 185

Query: 193 SMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHKI 252
            +    +S  +E+DV+     +T DVI+ TAFGSSY++G+ IF+LQ +   L +QAF K 
Sbjct: 186 KLFTDKES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKS 245

Query: 253 STDKNEYKFVEIRQGNKEI---IDEINRG--SKMEFRKELG 289
               + +   +  +  K I   +D I RG  SK E  +E G
Sbjct: 246 YIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAG 282

BLAST of Bhi02G000876 vs. TAIR 10
Match: AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )

HSP 1 Score: 167.2 bits (422), Expect = 2.0e-41
Identity = 86/211 (40.76%), Postives = 123/211 (58.29%), Query Frame = 0

Query: 36  WKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASSHPMPN--FSHNILPRVLSFYH 95
           W P RI+ HF +Q + GP YR F GN  EV  +   A S P+P+    H  + RV   YH
Sbjct: 24  WVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPIPSGRNPHEFVHRVAPHYH 83

Query: 96  HWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLK 155
            W ++YG TFL WFG    VA SDP +IRE  TT   F ++   +PL K L   GL  L+
Sbjct: 84  EWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRIGHNPLSKLLYAQGLPGLR 143

Query: 156 GQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQT 215
           G +WA HR+I    F M+ LK  +P +  S + ++EKW  M      +E+E++V +    
Sbjct: 144 GDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKWEDMRNG--GEEIELEVHKEMHN 203

Query: 216 LTEDVITKTAFGSSYEDGKAIFKLQTQQMIL 245
           L+ +++++TAFG+S E+GK IF+LQ + M L
Sbjct: 204 LSAEMLSRTAFGNSVEEGKGIFELQERMMRL 231

BLAST of Bhi02G000876 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 165.6 bits (418), Expect = 5.8e-41
Identity = 87/241 (36.10%), Postives = 145/241 (60.17%), Query Frame = 0

Query: 16  AAVTAAVVMILSA----KMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLA 75
           A VT +VV+++ +    + +  +W+KP+ +E +  RQG+ G PY   IG+ K  V+M+  
Sbjct: 6   ACVTVSVVVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLKRNVNMLTE 65

Query: 76  ASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHF 135
           A+S P+   + +I PRVL       K +G TF  W GP   + + DP++I+E+F    + 
Sbjct: 66  ATSKPI-KLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEVF-NKVYD 125

Query: 136 YEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKW 195
           Y K +   L+ +L   G++   G KWA HR+II+P FH++ +K ++P   +S  ++V +W
Sbjct: 126 YPKAQTF-LLGRLIATGIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSDVVGEW 185

Query: 196 SSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHK 253
           S +     S   E+DV+    ++T DVI++TAFGSSY++G+ IF+LQ + + L  QAF K
Sbjct: 186 SKLVSDKGSSSCEVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLILQAFWK 243

BLAST of Bhi02G000876 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 163.7 bits (413), Expect = 2.2e-40
Identity = 85/218 (38.99%), Postives = 132/218 (60.55%), Query Frame = 0

Query: 34  LWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYH 93
           +W+ P+ +E+   RQG+ G  Y   IG+FK+++SM + A+S P+   + +I PRV+    
Sbjct: 28  VWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKP-TDDITPRVMPHPL 87

Query: 94  HWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLK 153
              K +G T L WFGPI  + + DP+ I+E+F    + ++K    PL K L G GL++  
Sbjct: 88  QMLKTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQKAHTFPLSKIL-GTGLVSYD 147

Query: 154 GQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQT 213
           G KWA HR+II+P FH++ +K ++ +  ES  E+V +W  +  S      E+DV+    +
Sbjct: 148 GDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLV-SDKGSSCEVDVWPGLTS 207

Query: 214 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHK 252
           +T DVI++TAFGSSY +G  IF+LQ +   L  QAF K
Sbjct: 208 MTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQK 241

BLAST of Bhi02G000876 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.2e-93
Identity = 167/252 (66.27%), Postives = 211/252 (83.73%), Query Frame = 0

Query: 1   MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG 60
           M+ ES    +P V   +V  ++V++   K + +LWW+PR+IE+HF +QGIRGPPY FFIG
Sbjct: 1   MEEESSSWFIPKVLVLSVILSLVIV---KGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIG 60

Query: 61  NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           N KE+V MML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+
Sbjct: 61  NVKELVGMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDL 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV 180
           IREIF + S FYEKNEAHPLVKQLEGDGLL+LKG+KWA HRKIISPTFHM+NLKLL+P+V
Sbjct: 121 IREIF-SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVV 180

Query: 181 EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
            +SV +MV+KWS   + +++ EVE+DV+E FQ LTEDVI++TAFGSSYEDG+A+F+LQ Q
Sbjct: 181 LKSVTDMVDKWSD--KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQ 240

Query: 241 QMILASQAFHKI 253
           QM+L ++AF K+
Sbjct: 241 QMLLCAEAFQKV 245

BLAST of Bhi02G000876 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.3e-82
Identity = 140/241 (58.09%), Postives = 191/241 (79.25%), Query Frame = 0

Query: 14  WAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAAS 73
           WAAA  AA   + + K++ VLWW+PRR+E+HF RQGI GP YRF +G  +E+V++M+AAS
Sbjct: 6   WAAAAAAAAAWV-AVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAAS 65

Query: 74  SHPM--PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHF 133
           + PM  P  SHN+LPRVL+FYHHWKKIYGSTFLIWFGP  R+A++DP++IRE+    +  
Sbjct: 66  AKPMPPPYRSHNVLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADR 125

Query: 134 YEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKW 193
           +++ E+HP+V+QLEG+GL++L+G KWA HR++++P FHMDNL+LL+P V  +V++M +KW
Sbjct: 126 FDRYESHPMVRQLEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKW 185

Query: 194 SSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHK 253
            +MAE+  S EVEIDV + FQ +TED IT+TAFG SYEDGK +FKLQ Q M  AS+AF K
Sbjct: 186 RAMAEADKSGEVEIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRK 245

BLAST of Bhi02G000876 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.0e-74
Identity = 141/284 (49.65%), Postives = 193/284 (67.96%), Query Frame = 0

Query: 15  AAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASS 74
           AAA    V M ++A+M   LWW+PRR+E HF  QG+RGPPYRF +G+ +E+V++M  ASS
Sbjct: 22  AAAAAVWVTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASS 81

Query: 75  HPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYE 134
            PM P  SHN LPRVL+FYH+W+KIYG  FLIWFGP  R+ V++P++IREIF T +  ++
Sbjct: 82  KPMSPPTSHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFD 141

Query: 135 KNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSS 194
           + EAHP+V+QLEGDGL++L G KWALHR++++  F+ DNL  LIP V +SV  +  KW +
Sbjct: 142 RYEAHPVVRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGA 201

Query: 195 MAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHKI- 254
           MAE+  S EVE+DV E FQ +TE+ IT+  FG SY+DG+ +F +Q Q M  AS+AF K+ 
Sbjct: 202 MAEAGGSGEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVL 261

Query: 255 -------STDKNEYKF---VEIRQGNKEII----DEINRGSKME 283
                   T KN   +    EIR+    +I    DE  +G K +
Sbjct: 262 VPGYRFLPTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKAD 305

BLAST of Bhi02G000876 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.5e-70
Identity = 129/269 (47.96%), Postives = 188/269 (69.89%), Query Frame = 0

Query: 15  AAAVTAAVVMIL--SAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAA 74
           A AV AAV+++L  +A++   +WW+PRR+E HF  QG+RGPPYRF +G  +E+V++M  A
Sbjct: 4   AVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMAEA 63

Query: 75  SSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHF 134
           ++ PMP  + HN LPRVL+FYH+W+KIYG TFLIWFGP  R+ V++P+M+REIF T +  
Sbjct: 64  TAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRAEA 123

Query: 135 YEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKW 194
           +++ EAHP+V+QLEGDGL++L G KWA HR++++P F+ DNL  L+P V  SV  + E+W
Sbjct: 124 FDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAERW 183

Query: 195 SSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHK 254
            +MA      EVE+DV E FQ + E+ IT+  FG SY+ G+ +F+LQ + M  AS+AF K
Sbjct: 184 RAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAFRK 243

Query: 255 ISTDKNEYKFVEIRQGNKE--IIDEINRG 279
           +      Y+F+  ++      +  EI RG
Sbjct: 244 VLVP--GYRFLPTKKNRMSWGLDREIRRG 269

BLAST of Bhi02G000876 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.1e-59
Identity = 110/257 (42.80%), Postives = 169/257 (65.76%), Query Frame = 0

Query: 15  AAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASS 74
           AAA     V   +A+    LWW+PRR+E+HF  QG+RGP YRFF+G+  E+V +M+ A+S
Sbjct: 20  AAAAAWVAVYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAAS 79

Query: 75  HPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYE 134
            PM P  SH+ILPRVL FYHHW+K+YG   LIWFG   R+ VS+P++IRE+  T +  ++
Sbjct: 80  RPMEPPTSHDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFD 139

Query: 135 KNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSS 194
           + EAHP++ Q EG GL  L G++WA  R++++P FH +NL+++ P V  +V  M+++ + 
Sbjct: 140 RYEAHPMICQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAE 199

Query: 195 MAESTDSDEVEIDVFECFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQMILASQAFHKI 254
            A +  + E E+DV E FQ + ++ IT  AFG  +Y+DG A+F+LQ +    A++A  K+
Sbjct: 200 RARAGGAGEAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKV 259

Query: 255 STDKNEYKFVEIRQGNK 270
                 Y+F+  R+  +
Sbjct: 260 YIP--GYRFLPTRKNRR 274

BLAST of Bhi02G000876 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 251/252 (99.60%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG 60
           MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG
Sbjct: 1   MKIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIG 60

Query: 61  NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120
           NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM
Sbjct: 61  NFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120

Query: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV 180
           IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV
Sbjct: 121 IREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLV 180

Query: 181 EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240
           EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ
Sbjct: 181 EESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240

Query: 241 QMILASQAFHKI 253
           QMILASQAFHK+
Sbjct: 241 QMILASQAFHKV 252

BLAST of Bhi02G000876 vs. NCBI nr
Match: XP_011656192.2 (cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_019999 [Cucumis sativus])

HSP 1 Score: 437.2 bits (1123), Expect = 1.1e-118
Identity = 227/308 (73.70%), Postives = 253/308 (82.14%), Query Frame = 0

Query: 1   MKIESVLSKVPPVWAAAVTAAVVM---ILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRF 60
           +KIES      P W+A V   VV+   I++AK+ LVLWWKP++IEKHFDRQGI+GPPYRF
Sbjct: 17  VKIES------PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 76

Query: 61  FIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKE+VS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 77  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 136

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLI 180
           PDMIREIF TNSHFYEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH+DNLKLLI
Sbjct: 137 PDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 196

Query: 181 PLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES DS EVEIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 197 PLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 256

Query: 241 QTQQMILASQAFHKI----------STDKNEYK--------FVEIRQGNKEIIDEINRGS 288
           QTQQMILASQAF K+           T+ N +K         +++ +G K  +++ N G 
Sbjct: 257 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGM 316

BLAST of Bhi02G000876 vs. NCBI nr
Match: XP_008465003.1 (PREDICTED: cytochrome P450 734A1-like [Cucumis melo])

HSP 1 Score: 433.3 bits (1113), Expect = 1.6e-117
Identity = 218/255 (85.49%), Postives = 233/255 (91.37%), Query Frame = 0

Query: 1   MKIESVLSKVPPVW-AAAVTAAV--VMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRF 60
           +KIES      P W AAAVT AV  + I+SAK+ LVLWWKP++IEKHFDRQGI+GPPYRF
Sbjct: 4   VKIES------PAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKE+VS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLI 180
           PDMIREIF TNS +YEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH+DNLKLLI
Sbjct: 124 PDMIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES +S EVEIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFHKI 253
           Q QQMILASQAF K+
Sbjct: 244 QNQQMILASQAFQKV 252

BLAST of Bhi02G000876 vs. NCBI nr
Match: XP_022961116.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 419.5 bits (1077), Expect = 2.4e-113
Identity = 211/266 (79.32%), Postives = 226/266 (84.96%), Query Frame = 0

Query: 4   ESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFK 63
           E + S V   +  A  A VV+IL AKM LVLWWKPRRIE HF RQGI GPPYRFFIGNFK
Sbjct: 3   EELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFK 62

Query: 64  EVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRE 123
           E+VSMM AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIRE
Sbjct: 63  ELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIRE 122

Query: 124 IFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEES 183
           IF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPLVEES
Sbjct: 123 IFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEES 182

Query: 184 VVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMI 243
           V++MV+KWSSM E T S EVEIDVF+ FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMI
Sbjct: 183 VMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMI 242

Query: 244 LASQAFHKISTDKNEYKFVEIRQGNK 270
           LAS+AF K+      Y+F+  R   K
Sbjct: 243 LASEAFQKVFIP--GYRFLPTRTNMK 266

BLAST of Bhi02G000876 vs. NCBI nr
Match: XP_023516914.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.1 bits (1076), Expect = 3.1e-113
Identity = 210/255 (82.35%), Postives = 222/255 (87.06%), Query Frame = 0

Query: 15  AAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASS 74
           A A  AAVV+IL AK+ LVLWWKPRRIE HF RQGI GPPYRFFIGNFKE+VSMM AASS
Sbjct: 21  AVAAVAAVVVILGAKVGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFKELVSMMAAASS 80

Query: 75  HPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEK 134
           HPMPNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIREIF TNSH YEK
Sbjct: 81  HPMPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIPRVAVSDPDMIREIFITNSHLYEK 140

Query: 135 NEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSSM 194
           NEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPL+EES+VEMV+KWSSM
Sbjct: 141 NEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLIEESIVEMVQKWSSM 200

Query: 195 AESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHKIST 254
           AE T S EVEIDVF+ FQTLTEDVITKTAFG SY DGKAIFKLQTQQMILAS+AF K+  
Sbjct: 201 AEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYGDGKAIFKLQTQQMILASEAFQKVFI 260

Query: 255 DKNEYKFVEIRQGNK 270
               Y+F+  R   K
Sbjct: 261 P--GYRFLPTRTNMK 273

BLAST of Bhi02G000876 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 5.4e-119
Identity = 227/308 (73.70%), Postives = 253/308 (82.14%), Query Frame = 0

Query: 1   MKIESVLSKVPPVWAAAVTAAVVM---ILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRF 60
           +KIES      P W+A V   VV+   I++AK+ LVLWWKP++IEKHFDRQGI+GPPYRF
Sbjct: 4   VKIES------PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKE+VS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLI 180
           PDMIREIF TNSHFYEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH+DNLKLLI
Sbjct: 124 PDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES DS EVEIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFHKI----------STDKNEYK--------FVEIRQGNKEIIDEINRGS 288
           QTQQMILASQAF K+           T+ N +K         +++ +G K  +++ N G 
Sbjct: 244 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGM 303

BLAST of Bhi02G000876 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.8e-118
Identity = 218/255 (85.49%), Postives = 233/255 (91.37%), Query Frame = 0

Query: 1   MKIESVLSKVPPVW-AAAVTAAV--VMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRF 60
           +KIES      P W AAAVT AV  + I+SAK+ LVLWWKP++IEKHFDRQGI+GPPYRF
Sbjct: 4   VKIES------PAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 63

Query: 61  FIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120
           FIGNFKE+VS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 64  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 123

Query: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLI 180
           PDMIREIF TNS +YEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH+DNLKLLI
Sbjct: 124 PDMIREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 183

Query: 181 PLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240
           PLVEESVVEMVEKW SMAES +S EVEIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 184 PLVEESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 243

Query: 241 QTQQMILASQAFHKI 253
           Q QQMILASQAF K+
Sbjct: 244 QNQQMILASQAFQKV 252

BLAST of Bhi02G000876 vs. ExPASy TrEMBL
Match: A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.2e-113
Identity = 211/266 (79.32%), Postives = 226/266 (84.96%), Query Frame = 0

Query: 4   ESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFK 63
           E + S V   +  A  A VV+IL AKM LVLWWKPRRIE HF RQGI GPPYRFFIGNFK
Sbjct: 3   EELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNFK 62

Query: 64  EVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRE 123
           E+VSMM AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIRE
Sbjct: 63  ELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIRE 122

Query: 124 IFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEES 183
           IF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPLVEES
Sbjct: 123 IFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEES 182

Query: 184 VVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMI 243
           V++MV+KWSSM E T S EVEIDVF+ FQTLTEDVITKTAFG SYEDGKAIFK+QTQQMI
Sbjct: 183 VMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQMI 242

Query: 244 LASQAFHKISTDKNEYKFVEIRQGNK 270
           LAS+AF K+      Y+F+  R   K
Sbjct: 243 LASEAFQKVFIP--GYRFLPTRTNMK 266

BLAST of Bhi02G000876 vs. ExPASy TrEMBL
Match: A0A6J1JHK6 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.9e-111
Identity = 210/268 (78.36%), Postives = 225/268 (83.96%), Query Frame = 0

Query: 2   KIESVLSKVPPVWAAAVTAAVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGN 61
           ++ES +     V AAAV AA V+IL AKM LVLWWKPRR+E HF RQGI GP YRFFIGN
Sbjct: 4   ELESAVEVPFGVAAAAVVAAAVVILGAKMGLVLWWKPRRMENHFLRQGIGGPRYRFFIGN 63

Query: 62  FKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMI 121
           FKE+VSMM AASSHPMP FSHNILPRVLSFYHHWKKIYGSTFLIWFGPI RVAVSDPDMI
Sbjct: 64  FKELVSMMAAASSHPMPKFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIARVAVSDPDMI 123

Query: 122 REIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVE 181
           REIF TNSH YEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DN+KLLIPL+E
Sbjct: 124 REIFITNSHLYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHSDNIKLLIPLIE 183

Query: 182 ESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQ 241
           E +VEMV+KWSSMAE T    VEIDVF+ FQTLTEDVITKTAFG SYEDGKAIFKLQTQQ
Sbjct: 184 EIIVEMVQKWSSMAEETTLGGVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKLQTQQ 243

Query: 242 MILASQAFHKISTDKNEYKFVEIRQGNK 270
           MILAS+AF K+      Y+F+  R   K
Sbjct: 244 MILASEAFQKVFIP--GYRFLPTRTNMK 269

BLAST of Bhi02G000876 vs. ExPASy TrEMBL
Match: A0A6J1L528 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.9e-108
Identity = 202/263 (76.81%), Postives = 225/263 (85.55%), Query Frame = 0

Query: 21  AVVMILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRFFIGNFKEVVSMMLAASSHPMPNF 80
           A++ IL+ KM + LWWKPRRIE HF RQGIRGPPYRFFIGNFKE+ SMMLAA+S PMPNF
Sbjct: 19  ALLFILAVKMAVALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDSMMLAATSRPMPNF 78

Query: 81  SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFTTNSHFYEKNEAHPL 140
           SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+I TTNS FYEKNEAHPL
Sbjct: 79  SHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTTNSEFYEKNEAHPL 138

Query: 141 VKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLIPLVEESVVEMVEKWSSMAESTDS 200
           +K+LEGDGLLTLK QKWALHRKIISP F+M+NLKLLIP+VEESVV+MVEKWS+MA    S
Sbjct: 139 IKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDMVEKWSAMAGGAAS 198

Query: 201 DEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFHKISTDKNEYK 260
           DEVEIDVF+ FQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILAS+AF K+      Y+
Sbjct: 199 DEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASEAFQKVFIP--GYR 258

Query: 261 FVEIRQG-NKEIIDEINRGSKME 283
           F+  R   N   +D+  R S M+
Sbjct: 259 FLPTRTNINSWKLDKEIRKSLMK 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G26710.11.6e-9466.27Cytochrome P450 superfamily protein [more]
AT3G14610.14.0e-4237.01cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT1G75130.12.0e-4140.76cytochrome P450, family 721, subfamily A, polypeptide 1 [more]
AT3G14640.15.8e-4136.10cytochrome P450, family 72, subfamily A, polypeptide 10 [more]
AT3G14680.12.2e-4038.99cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
Match NameE-valueIdentityDescription
O487862.2e-9366.27Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
B9X2872.3e-8258.09Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Q6Z6D63.0e-7449.65Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
Q69XM64.5e-7047.96Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... [more]
Q8LIF26.1e-5942.80Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_038880221.11.1e-13999.60cytochrome P450 734A1-like [Benincasa hispida][more]
XP_011656192.21.1e-11873.70cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_0... [more]
XP_008465003.11.6e-11785.49PREDICTED: cytochrome P450 734A1-like [Cucumis melo][more]
XP_022961116.12.4e-11379.32cytochrome P450 734A1-like [Cucurbita moschata][more]
XP_023516914.13.1e-11382.35cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LU205.4e-11973.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1[more]
A0A1S3CMW07.8e-11885.49cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1[more]
A0A6J1H9G11.2e-11379.32cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV... [more]
A0A6J1JHK61.9e-11178.36cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1[more]
A0A6J1L5281.9e-10876.81cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111499208 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002402Cytochrome P450, E-class, group IIPRINTSPR00464EP450IIcoord: 213..231
score: 33.21
coord: 154..174
score: 37.41
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 34..286
e-value: 5.2E-37
score: 129.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 45..246
IPR001128Cytochrome P450PFAMPF00067p450coord: 89..249
e-value: 3.1E-21
score: 75.7
NoneNo IPR availablePANTHERPTHR24282:SF63CYTOCHROMEA2coord: 13..262
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 13..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000876Bhi02M000876mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016131 brassinosteroid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen