Bhi02G000875 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000875
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCytochrome P450 protein
Locationchr2: 23507745 .. 23508876 (-)
RNA-Seq ExpressionBhi02G000875
SyntenyBhi02G000875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCCACAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAACATGATGTCTCTAAATTCAAAATCGTAAGCCCTTTTTTTCTCTTTTCCTATTTCCATTTCATTATTTCAAAATTAAAATAAATATTAAAAATAATAACCCCTATATTCCATTTTAATTCTGGTACTTTCAATTGTTTAATTTTAGTTTTTCATATTTTTAATAAATCTTAAATTTAGTTTTTCAAAGTTAACTTATAGTGAGGTTGATTAAATAATAATATTAATAATTTTCAGGTAAGAAAATAGAACATTTGAATTTGTTGTCAAAATTTGTAATAAAACTATTATTAAATTTTTTTAAAAAATCAACAATAAACTAAATTTTAGATTTATTAAAATTACAAACACTAAAATTGCGCAATTGAAAATACATGAACTAAAAGTATAAAAACCAAAATATTGTTTTAACCTTGTAATAATAATAACTAATTTGTTTAAGAAATAAATTATTATTGAAAATAGAGGGTAAAAAAGAAAAGAAAAGAAAAAAGTTTTAGAAAATTAATTGTTTTGTCATTTTACTAATAAAAACTAATAAACTGCCAACTTTTTGGTTTACTTTGCTTGGTTTGTTTAATGGAGATCTTTTTTGTGTTGGTGGGGAAATGATTGGGTGTGGCAGCTGAGCATGATACTAAATGAATCCTTGCGATTATACCCACCCACGGTGGCAACAATCCGACGTGCTAAGGCTGACGTGGAACTAGGTGGACGAATGATCCCACGTGGCACGGAGTTTTTGATTCCAATCTTAGCTGTTCATCATGATCAAAGTATTTGGGGGAATGATGCGAGCGAATTCAACCCAGGGCGGTTCTCGGAAGGAGTGGGTCGAGCCGGAAACCACCCGGCCGGGTTTATTCCGTTTGGACTCGGGGCTCGTACTTGCATTGGCCAAAATCTGGCCATTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAGCATGCACCAACGGTTCTGATGCTTCTTTATCCACAATATGGGGCCCCCATTCTTTTTCAAAAGATACCCCTAGACTAA

mRNA sequence

ATGCATCCACAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAACATGATGTCTCTAAATTCAAAATCCTGAGCATGATACTAAATGAATCCTTGCGATTATACCCACCCACGGTGGCAACAATCCGACGTGCTAAGGCTGACGTGGAACTAGGTGGACGAATGATCCCACGTGGCACGGAGTTTTTGATTCCAATCTTAGCTGTTCATCATGATCAAAGTATTTGGGGGAATGATGCGAGCGAATTCAACCCAGGGCGGTTCTCGGAAGGAGTGGGTCGAGCCGGAAACCACCCGGCCGGGTTTATTCCGTTTGGACTCGGGGCTCGTACTTGCATTGGCCAAAATCTGGCCATTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAGCATGCACCAACGGTTCTGATGCTTCTTTATCCACAATATGGGGCCCCCATTCTTTTTCAAAAGATACCCCTAGACTAA

Coding sequence (CDS)

ATGCATCCACAGTGGCAAATTCAGGCACGTGATGAGGTCTTTAACGTCTGCGGTTCACGTGCCCTCCCTTCCAAACATGATGTCTCTAAATTCAAAATCCTGAGCATGATACTAAATGAATCCTTGCGATTATACCCACCCACGGTGGCAACAATCCGACGTGCTAAGGCTGACGTGGAACTAGGTGGACGAATGATCCCACGTGGCACGGAGTTTTTGATTCCAATCTTAGCTGTTCATCATGATCAAAGTATTTGGGGGAATGATGCGAGCGAATTCAACCCAGGGCGGTTCTCGGAAGGAGTGGGTCGAGCCGGAAACCACCCGGCCGGGTTTATTCCGTTTGGACTCGGGGCTCGTACTTGCATTGGCCAAAATCTGGCCATTTTGGAAGCAAAATTAACAATGGCAATTATACTTCAACGGTTCACGTTTCGTTTGGCTCCCACTTATCAGCATGCACCAACGGTTCTGATGCTTCTTTATCCACAATATGGGGCCCCCATTCTTTTTCAAAAGATACCCCTAGACTAA

Protein sequence

MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGARTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD
Homology
BLAST of Bhi02G000875 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 5.8e-76
Identity = 131/174 (75.29%), Postives = 151/174 (86.78%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHP+WQ +ARDEV  VCGSR +P+K  V K K LSMILNESLRLYPP VATIRRAK+DV+
Sbjct: 342 MHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVK 401

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LGG  IP GTE LIPI+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLG R
Sbjct: 402 LGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVR 461

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
           TCIGQNLAIL+AKLT+A+++QRFTF LAPTYQHAPTVLMLLYPQ+GAPI F+++
Sbjct: 462 TCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Bhi02G000875 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 179.5 bits (454), Expect = 2.4e-45
Identity = 79/174 (45.40%), Postives = 113/174 (64.94%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           +H  WQ + R+EVFN CG   +P     SK K+++ +  ESLRLY P +  +R A  D++
Sbjct: 397 LHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMK 456

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LG   IP+GT  ++PI  +H D+++WG+DA +FNP RF+ G+ RA NHP   + F +G R
Sbjct: 457 LGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPR 516

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
            CIGQN AI+EAK  +A+ILQRF   L+  Y+HAP   + L PQY  P++ + I
Sbjct: 517 ACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPI 570

BLAST of Bhi02G000875 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 178.7 bits (452), Expect = 4.1e-45
Identity = 80/174 (45.98%), Postives = 117/174 (67.24%), Query Frame = 0

Query: 2   HPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVEL 61
           H +WQ QAR+E+  V G    P+   +S+ K +SMILNE LRLYPP +   R  + + +L
Sbjct: 342 HQKWQDQAREEILKVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKL 401

Query: 62  GGRM-IPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 121
           G  M +P G + +IP+L VH D  +WG D  EFNP RF++G+ +A  +   F+PFG G R
Sbjct: 402 GEDMTLPGGAQVVIPVLMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPR 461

Query: 122 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
            C GQN A++EAK+ + +ILQRF+F L+P+Y HAP  ++ L+PQ+GAP++F  +
Sbjct: 462 FCPGQNFALMEAKMALVLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

BLAST of Bhi02G000875 vs. TAIR 10
Match: AT2G46960.1 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.3e-43
Identity = 77/175 (44.00%), Postives = 111/175 (63.43%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           +H  WQ + R+E+F  CG    P     SK K+++M++ ESLRLY P  A  R A  +++
Sbjct: 227 LHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIK 286

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LG   IP+GT  +IP+L +H D+++WG+DA +FNP RF+ GV RA NHP   + F +G R
Sbjct: 287 LGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPR 346

Query: 121 TCIGQNLAILEAKLTMAIILQRFTF-RLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
            CIGQN  ++EAK  + +ILQRF F  L   Y+H P   + + PQYG P++ Q +
Sbjct: 347 ACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 401

BLAST of Bhi02G000875 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 173.7 bits (439), Expect = 1.3e-43
Identity = 77/175 (44.00%), Postives = 111/175 (63.43%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           +H  WQ + R+E+F  CG    P     SK K+++M++ ESLRLY P  A  R A  +++
Sbjct: 343 LHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIK 402

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LG   IP+GT  +IP+L +H D+++WG+DA +FNP RF+ GV RA NHP   + F +G R
Sbjct: 403 LGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPR 462

Query: 121 TCIGQNLAILEAKLTMAIILQRFTF-RLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
            CIGQN  ++EAK  + +ILQRF F  L   Y+H P   + + PQYG P++ Q +
Sbjct: 463 ACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Bhi02G000875 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 8.2e-75
Identity = 131/174 (75.29%), Postives = 151/174 (86.78%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHP+WQ +ARDEV  VCGSR +P+K  V K K LSMILNESLRLYPP VATIRRAK+DV+
Sbjct: 342 MHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVK 401

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LGG  IP GTE LIPI+AVHHDQ+IWGND +EFNP RF++GV RA  HP GFIPFGLG R
Sbjct: 402 LGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVR 461

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
           TCIGQNLAIL+AKLT+A+++QRFTF LAPTYQHAPTVLMLLYPQ+GAPI F+++
Sbjct: 462 TCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Bhi02G000875 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.1e-66
Identity = 121/173 (69.94%), Postives = 143/173 (82.66%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHP+WQ +AR EV +VCG+  +PS+  ++K K L MILNE+LRLYPP VAT+RRAKADVE
Sbjct: 352 MHPEWQERARQEVLDVCGADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVE 411

Query: 61  LGGRM-IPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGA 120
           LGG + IPR TE LIPI+AVHHD  +WG DA++FNP RF+ GV RA  HPA FIPFGLGA
Sbjct: 412 LGGYLRIPRDTELLIPIMAVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGA 471

Query: 121 RTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQ 173
           R CIGQNLAILEAKLT+A+IL RF FRL+  Y HAPTVLMLL+PQYGAPI+F+
Sbjct: 472 RMCIGQNLAILEAKLTVAVILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524

BLAST of Bhi02G000875 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 8.5e-64
Identity = 115/175 (65.71%), Postives = 136/175 (77.71%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHP WQ +AR EVF+VCG+  LPSK  + K K L MI+NE+LRLYPP VATIRRAK DV+
Sbjct: 373 MHPDWQERARREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQ 432

Query: 61  LG-GRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGA 120
           L  G MIPR  E L+PI+A+HHD   WG DAS+FNP RF+ G  +A  HP  FIPFGLG+
Sbjct: 433 LSDGCMIPRDMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGS 492

Query: 121 RTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
           R C+GQNLA LEAKLTMAI+LQRF  R +P Y HAPTVLMLLYPQYGAP++F+ +
Sbjct: 493 RMCVGQNLARLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPL 547

BLAST of Bhi02G000875 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.9e-61
Identity = 116/182 (63.74%), Postives = 136/182 (74.73%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRA--LPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKAD 60
           MHP WQ +AR EV  VCG  A  LP+K  + K K L MILNE+LRLYPP VATIRRAK D
Sbjct: 349 MHPDWQDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFD 408

Query: 61  VELGGR--------MIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPA 120
           V LGG          IPR TE L+PI+A+HHD  +WG DA++FNP RF+ G  RA  HP 
Sbjct: 409 VTLGGGGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPL 468

Query: 121 GFIPFGLGARTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIL 173
            FIPFGLG+R CIGQ+LAILEAKLTMA++LQRF   L+PTY HAPTVLMLL+PQYGAP++
Sbjct: 469 AFIPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLI 528

BLAST of Bhi02G000875 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.2e-57
Identity = 104/175 (59.43%), Postives = 134/175 (76.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHP+WQ +AR EV  VCG   LP+K  + K K L MILNE+LRLYPP VA IR AK DVE
Sbjct: 358 MHPEWQERARREVVAVCGRGDLPTKDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVE 417

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRF-SEGVGRAGNHPAGFIPFGLGA 120
           LGG ++P GTE +IPI+AVHHD + WG+DA+EFNP RF ++  G    HP  F+PFG GA
Sbjct: 418 LGGCVVPAGTEVMIPIMAVHHDAAAWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGA 477

Query: 121 RTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKI 175
           R CIGQN+A++EAK+ +A++L+RF FRL+P Y HAP VLM+L PQ+GAP++F+ +
Sbjct: 478 RVCIGQNMALMEAKVALAVVLRRFEFRLSPAYVHAPRVLMILSPQFGAPVIFRPL 532

BLAST of Bhi02G000875 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 363.6 bits (932), Expect = 9.6e-97
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 359 MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 418

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR
Sbjct: 419 LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 478

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 178
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD
Sbjct: 479 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 535

BLAST of Bhi02G000875 vs. NCBI nr
Match: TYK14516.1 (cytochrome P450 734A1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 6.0e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 188 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 247

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 248 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 307

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 308 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 362

BLAST of Bhi02G000875 vs. NCBI nr
Match: XP_008465003.1 (PREDICTED: cytochrome P450 734A1-like [Cucumis melo])

HSP 1 Score: 344.4 bits (882), Expect = 6.0e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 363 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 422

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 423 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 482

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 483 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of Bhi02G000875 vs. NCBI nr
Match: KAA0046739.1 (cytochrome P450 734A1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 6.0e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 273 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 332

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 333 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 392

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 393 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 447

BLAST of Bhi02G000875 vs. NCBI nr
Match: XP_011656192.2 (cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_019999 [Cucumis sativus])

HSP 1 Score: 341.3 bits (874), Expect = 5.1e-90
Identity = 162/177 (91.53%), Postives = 170/177 (96.05%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEVFNVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAK DVE
Sbjct: 371 MHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 430

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLG R
Sbjct: 431 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVR 490

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 178
           TCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP++
Sbjct: 491 TCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 547

BLAST of Bhi02G000875 vs. ExPASy TrEMBL
Match: A0A5D3CVT3 (Cytochrome P450 734A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold15G00310 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.9e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 188 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 247

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 248 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 307

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 308 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 362

BLAST of Bhi02G000875 vs. ExPASy TrEMBL
Match: A0A5A7TZS8 (Cytochrome P450 734A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00200 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.9e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 273 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 332

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 333 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 392

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 393 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 447

BLAST of Bhi02G000875 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 2.9e-91
Identity = 166/175 (94.86%), Postives = 169/175 (96.57%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEV NVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAKADVE
Sbjct: 363 MHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKADVE 422

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV RAGNHPAGFIPFGLG R
Sbjct: 423 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLGVR 482

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIP 176
           TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP
Sbjct: 483 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of Bhi02G000875 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.5e-90
Identity = 162/177 (91.53%), Postives = 170/177 (96.05%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQIQARDEVFNVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAK DVE
Sbjct: 358 MHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 417

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           L GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLG R
Sbjct: 418 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVR 477

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 178
           TCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP++
Sbjct: 478 TCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 534

BLAST of Bhi02G000875 vs. ExPASy TrEMBL
Match: A0A6J1JHK6 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.0e-84
Identity = 155/177 (87.57%), Postives = 162/177 (91.53%), Query Frame = 0

Query: 1   MHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKADVE 60
           MHPQWQI AR EVF +CG RALPS  DVSKFKILSMILNESLRLYPPTVATIRRA AD+E
Sbjct: 348 MHPQWQILARHEVFTICGPRALPSPDDVSKFKILSMILNESLRLYPPTVATIRRANADME 407

Query: 61  LGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLGAR 120
           LGGRMIPRGTEFLIPILAVHHDQ+IWGNDASEFNP RFS+GV RAGNHP GFIPFGLG R
Sbjct: 408 LGGRMIPRGTEFLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVR 467

Query: 121 TCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 178
            CIGQNLAILEAKL M IILQR +FRLAPTYQHAPTVLMLLYPQYGAPILF+KIPL+
Sbjct: 468 NCIGQNLAILEAKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIPLN 524

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G26710.15.8e-7675.29Cytochrome P450 superfamily protein [more]
AT2G46950.12.4e-4545.40cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT3G14620.14.1e-4545.98cytochrome P450, family 72, subfamily A, polypeptide 8 [more]
AT2G46960.11.3e-4344.00cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G46960.21.3e-4344.00cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
Match NameE-valueIdentityDescription
O487868.2e-7575.29Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
B9X2871.1e-6669.94Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Q6Z6D68.5e-6465.71Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
Q69XM63.9e-6163.74Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... [more]
Q8LIF21.2e-5759.43Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_038880221.19.6e-97100.00cytochrome P450 734A1-like [Benincasa hispida][more]
TYK14516.16.0e-9194.86cytochrome P450 734A1-like protein [Cucumis melo var. makuwa][more]
XP_008465003.16.0e-9194.86PREDICTED: cytochrome P450 734A1-like [Cucumis melo][more]
KAA0046739.16.0e-9194.86cytochrome P450 734A1-like protein [Cucumis melo var. makuwa][more]
XP_011656192.25.1e-9091.53cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_0... [more]
Match NameE-valueIdentityDescription
A0A5D3CVT32.9e-9194.86Cytochrome P450 734A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7TZS82.9e-9194.86Cytochrome P450 734A1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3CMW02.9e-9194.86cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1[more]
A0A0A0LU202.5e-9091.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1[more]
A0A6J1JHK62.0e-8487.57cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 122..133
score: 43.47
coord: 36..47
score: 39.28
coord: 113..122
score: 56.5
coord: 1..13
score: 25.96
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..155
e-value: 4.6E-44
score: 150.9
IPR002397Cytochrome P450, B-classPRINTSPR00359BP450coord: 36..47
score: 30.19
coord: 53..80
score: 29.52
coord: 113..122
score: 41.33
coord: 122..133
score: 51.67
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..174
e-value: 2.0E-53
score: 183.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..172
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 1..169
NoneNo IPR availablePANTHERPTHR24282:SF63CYTOCHROMEA2coord: 1..169
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 115..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000875Bhi02M000875mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016131 brassinosteroid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen