Bhi02G000852 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000852
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMAPK kinase substrate protein At1g80180-like
Locationchr2: 22893932 .. 22894123 (+)
RNA-Seq ExpressionBhi02G000852
SyntenyBhi02G000852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGATTGCAGAGGTCCGCCGTGTCGTTCCGGCGACAGGGCTCCTCCGGCTTGGTGTGGGACGACAGATTCTTCAGCGGCGAATTGAAACCGACAAAACGCCGCCATGAAGAACAATACAACGTGGTTTTTCTTTCCGGTGAGTTTGAGGCCTTTCTCGAAGCATCGGATCGGGCGGCGCCACCTTGA

mRNA sequence

ATGGCCGGATTGCAGAGGTCCGCCGTGTCGTTCCGGCGACAGGGCTCCTCCGGCTTGGTGTGGGACGACAGATTCTTCAGCGGCGAATTGAAACCGACAAAACGCCGCCATGAAGAACAATACAACGTGGTTTTTCTTTCCGGTGAGTTTGAGGCCTTTCTCGAAGCATCGGATCGGGCGGCGCCACCTTGA

Coding sequence (CDS)

ATGGCCGGATTGCAGAGGTCCGCCGTGTCGTTCCGGCGACAGGGCTCCTCCGGCTTGGTGTGGGACGACAGATTCTTCAGCGGCGAATTGAAACCGACAAAACGCCGCCATGAAGAACAATACAACGTGGTTTTTCTTTCCGGTGAGTTTGAGGCCTTTCTCGAAGCATCGGATCGGGCGGCGCCACCTTGA

Protein sequence

MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEEQYNVVFLSGEFEAFLEASDRAAPP
Homology
BLAST of Bhi02G000852 vs. TAIR 10
Match: AT5G20100.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 82; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 1.2e-07
Identity = 22/35 (62.86%), Postives = 29/35 (82.86%), Query Frame = 0

Query: 4  LQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHE 39
          LQRS  SFRRQGSSGL+W+DRF SGE++  +R+ +
Sbjct: 3  LQRSTASFRRQGSSGLIWNDRFLSGEIRNDERQED 37

BLAST of Bhi02G000852 vs. TAIR 10
Match: AT1G80180.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15400.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFS 28
          MAGLQRS +SFRRQGSSG+VWDDR  +
Sbjct: 1  MAGLQRSTISFRRQGSSGIVWDDRLIA 27

BLAST of Bhi02G000852 vs. TAIR 10
Match: AT1G15400.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 43.5 bits (101), Expect = 7.3e-05
Identity = 19/27 (70.37%), Postives = 23/27 (85.19%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFS 28
          M GLQRS +SFRRQGSSG+V+DDR  +
Sbjct: 1  MEGLQRSTISFRRQGSSGIVFDDRLIA 27

BLAST of Bhi02G000852 vs. TAIR 10
Match: AT1G15400.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 82 Blast hits to 82 proteins in 16 species: Archae - 0; Bacteria - 4; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 43.5 bits (101), Expect = 7.3e-05
Identity = 19/27 (70.37%), Postives = 23/27 (85.19%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFS 28
          M GLQRS +SFRRQGSSG+V+DDR  +
Sbjct: 1  MEGLQRSTISFRRQGSSGIVFDDRLIA 27

BLAST of Bhi02G000852 vs. TAIR 10
Match: AT1G15400.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G80180.1); Has 84 Blast hits to 84 proteins in 17 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 75; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 43.5 bits (101), Expect = 7.3e-05
Identity = 19/27 (70.37%), Postives = 23/27 (85.19%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFS 28
          M GLQRS +SFRRQGSSG+V+DDR  +
Sbjct: 1  MEGLQRSTISFRRQGSSGIVFDDRLIA 27

BLAST of Bhi02G000852 vs. ExPASy Swiss-Prot
Match: Q9SSC1 (MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g80180 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.9e-05
Identity = 21/27 (77.78%), Postives = 24/27 (88.89%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFS 28
          MAGLQRS +SFRRQGSSG+VWDDR  +
Sbjct: 1  MAGLQRSTISFRRQGSSGIVWDDRLIA 27

BLAST of Bhi02G000852 vs. NCBI nr
Match: XP_008465027.1 (PREDICTED: uncharacterized protein At1g15400 [Cucumis melo] >KAA0046761.1 uncharacterized protein E6C27_scaffold216G00440 [Cucumis melo var. makuwa] >TYK29686.1 uncharacterized protein E5676_scaffold64G00370 [Cucumis melo var. makuwa])

HSP 1 Score: 91.3 bits (225), Expect = 3.3e-15
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEEQYNVVFLSGE 50
          MA LQRSAVSFRRQGSSGLVWDDRF SGELKPTKR HE+Q+NVVFLSGE
Sbjct: 1  MAELQRSAVSFRRQGSSGLVWDDRFLSGELKPTKRFHEDQHNVVFLSGE 49

BLAST of Bhi02G000852 vs. NCBI nr
Match: XP_004151607.1 (uncharacterized protein At1g15400 [Cucumis sativus] >KGN65310.1 hypothetical protein Csa_020108 [Cucumis sativus])

HSP 1 Score: 88.6 bits (218), Expect = 2.1e-14
Identity = 46/63 (73.02%), Postives = 48/63 (76.19%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKP-TKRRHEEQYNVVFLSGEFEAFLEASDR 60
          MA LQRSAVSFRRQGSSGLVWDDRF SGELKP TKR HE+Q+NVVFLSGE          
Sbjct: 1  MAELQRSAVSFRRQGSSGLVWDDRFLSGELKPTTKRLHEDQHNVVFLSGELRPSRSVRSG 60

Query: 61 AAP 63
           AP
Sbjct: 61 GAP 63

BLAST of Bhi02G000852 vs. NCBI nr
Match: XP_022960593.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita moschata] >KAG6589861.1 hypothetical protein SDJN03_15284, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 85.1 bits (209), Expect = 2.3e-13
Identity = 43/51 (84.31%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEE--QYNVVFLSGE 50
          MAGLQRSAVSFRRQGSSGLVWDDRFFSGEL PT RRHEE  Q + +FLSGE
Sbjct: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELTPTMRRHEEQPQRDALFLSGE 51

BLAST of Bhi02G000852 vs. NCBI nr
Match: KAG7023533.1 (hypothetical protein SDJN02_14559, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 85.1 bits (209), Expect = 2.3e-13
Identity = 43/51 (84.31%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEE--QYNVVFLSGE 50
          MAGLQRSAVSFRRQGSSGLVWDDRFFSGEL PT RRHEE  Q + +FLSGE
Sbjct: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELTPTMRRHEEQPQRDALFLSGE 51

BLAST of Bhi02G000852 vs. NCBI nr
Match: XP_023516734.1 (MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 84.0 bits (206), Expect = 5.2e-13
Identity = 42/51 (82.35%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEE--QYNVVFLSGE 50
          MAGLQRSAVSFRRQGSSGLVWDDR+FSGEL PT RRHEE  Q + +FLSGE
Sbjct: 1  MAGLQRSAVSFRRQGSSGLVWDDRYFSGELTPTMRRHEEQPQRDALFLSGE 51

BLAST of Bhi02G000852 vs. ExPASy TrEMBL
Match: A0A5A7TZI7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold64G00370 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.6e-15
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEEQYNVVFLSGE 50
          MA LQRSAVSFRRQGSSGLVWDDRF SGELKPTKR HE+Q+NVVFLSGE
Sbjct: 1  MAELQRSAVSFRRQGSSGLVWDDRFLSGELKPTKRFHEDQHNVVFLSGE 49

BLAST of Bhi02G000852 vs. ExPASy TrEMBL
Match: A0A1S3CMU7 (uncharacterized protein At1g15400 OS=Cucumis melo OX=3656 GN=LOC103502744 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.6e-15
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEEQYNVVFLSGE 50
          MA LQRSAVSFRRQGSSGLVWDDRF SGELKPTKR HE+Q+NVVFLSGE
Sbjct: 1  MAELQRSAVSFRRQGSSGLVWDDRFLSGELKPTKRFHEDQHNVVFLSGE 49

BLAST of Bhi02G000852 vs. ExPASy TrEMBL
Match: A0A0A0LTQ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G308810 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.0e-14
Identity = 46/63 (73.02%), Postives = 48/63 (76.19%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKP-TKRRHEEQYNVVFLSGEFEAFLEASDR 60
          MA LQRSAVSFRRQGSSGLVWDDRF SGELKP TKR HE+Q+NVVFLSGE          
Sbjct: 1  MAELQRSAVSFRRQGSSGLVWDDRFLSGELKPTTKRLHEDQHNVVFLSGELRPSRSVRSG 60

Query: 61 AAP 63
           AP
Sbjct: 61 GAP 63

BLAST of Bhi02G000852 vs. ExPASy TrEMBL
Match: A0A6J1H9E8 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LOC111461328 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-13
Identity = 43/51 (84.31%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEE--QYNVVFLSGE 50
          MAGLQRSAVSFRRQGSSGLVWDDRFFSGEL PT RRHEE  Q + +FLSGE
Sbjct: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELTPTMRRHEEQPQRDALFLSGE 51

BLAST of Bhi02G000852 vs. ExPASy TrEMBL
Match: A0A6J1JFP4 (MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC111485313 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.3e-13
Identity = 42/51 (82.35%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 1  MAGLQRSAVSFRRQGSSGLVWDDRFFSGELKPTKRRHEE--QYNVVFLSGE 50
          MAGLQRSAVSFRRQGSSGLVW+DRFFSGEL PT RRHEE  Q + +FLSGE
Sbjct: 1  MAGLQRSAVSFRRQGSSGLVWEDRFFSGELTPTMRRHEEQPQRDALFLSGE 51

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G20100.11.2e-0762.86unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G80180.11.3e-0677.78unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G15400.17.3e-0570.37unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G15400.27.3e-0570.37unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G15400.37.3e-0570.37unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
Match NameE-valueIdentityDescription
Q9SSC11.9e-0577.78MAPK kinase substrate protein At1g80180 OS=Arabidopsis thaliana OX=3702 GN=At1g8... [more]
Match NameE-valueIdentityDescription
XP_008465027.13.3e-1589.80PREDICTED: uncharacterized protein At1g15400 [Cucumis melo] >KAA0046761.1 unchar... [more]
XP_004151607.12.1e-1473.02uncharacterized protein At1g15400 [Cucumis sativus] >KGN65310.1 hypothetical pro... [more]
XP_022960593.12.3e-1384.31MAPK kinase substrate protein At1g80180-like [Cucurbita moschata] >KAG6589861.1 ... [more]
KAG7023533.12.3e-1384.31hypothetical protein SDJN02_14559, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023516734.15.2e-1382.35MAPK kinase substrate protein At1g80180-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5A7TZI71.6e-1589.80Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CMU71.6e-1589.80uncharacterized protein At1g15400 OS=Cucumis melo OX=3656 GN=LOC103502744 PE=4 S... [more]
A0A0A0LTQ51.0e-1473.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G308810 PE=4 SV=1[more]
A0A6J1H9E81.1e-1384.31MAPK kinase substrate protein At1g80180-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JFP43.3e-1382.35MAPK kinase substrate protein At1g80180-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031421Protein of unknown function DUF4666PFAMPF15697DUF4666coord: 1..41
e-value: 1.1E-15
score: 58.4
IPR031421Protein of unknown function DUF4666PANTHERPTHR33730OS05G0542732 PROTEIN-RELATEDcoord: 1..42

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000852Bhi02M000852mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity