Bhi02G000754 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000754
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr2: 20287232 .. 20289472 (+)
RNA-Seq ExpressionBhi02G000754
SyntenyBhi02G000754
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTTTTTCTTTGAAATTTTAGAGTTATTTCTGCAACGACTTTGTTTATATAATAACAATAAGTATATATTTTTAACACTTTTAAGTTTATGAATATTTTTTAAAACTTTAGAAATTTCAGCACTCTAACATTTTTCATCCAAATCTAAACGTAGGAGAGCAGCGGTGTCGATGAAACCTGATCCATCGTAACAGAGGATGTCTCTTTTTTCCCCCTGCATTTCATTTGTTCCAGAGGCGACGAAATGTATGATCGATGTGTTTAAGCACCCAGTTGCTCCTCATCCCGCTTGTTGCAATGCCATTTCTAAGCTCAAGAACTGTTCCTCTTCGTTTTTCAATGGCATTCCTTCTGCTGATATGATTCTGACTAAGAGTGTTTGTGCTTCGTGGGGGGCTCCAATTTCTTAGTCGAATATGAATTTTGTGATAATAAGATCTGTTCTGGGAATGGGCTTATGTTTTTCTTTGGGTTAATGGAGAAATTGTCGATTGAAAAGTCAAATTCTTCATTTTTCTTCTTGCAGTCTTTAATATTATATTATAATTTTATAAGGCTCTGATTCAAGGCTGTCTATTTCTCGCGATTGCTGTTGAGAACTCCAATTTTAAATCTAAACCCATTACAATGCTCTGTGTTTGATTGATCTCTGTTTTGCAACCGGAACTGCAGAAAGCCGTCGTTTCTTGACCACCAGCCATGGCCGGAACTAATCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTGAACGCTCCGTTAATGGCAGCTCCTGTAATCGATATCGGTCTCTTCTCATCCGGACCGGAACTGGAGAAACTCCGCCATGGACTTCAATCATGGGGCTGCTTTCAGGTTCGATTCTCTTGATTTTGCTCTCTAAAATCCTAAAACCTCACAAGGGTTAGGAATTTCTTTAAACCCTAATTTCTGTGGACTCTGTATCTCGATCCCATTAAAAATCAAATTGAAGAAACTAGTAAGTGTTTGTGGTGTTTCTATAGGCTATAAATCATGGAATGTCAACTGAATTTCTTGATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGGAAGAGAAACTGAAATACTCAAGAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAGACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATGTCCACAACAAAGCCGTCGTTTTAAGTATTGGCCAACAAATCCTGAAAGATTTAGGTACTTTTTTCTTTCTCAAATCTTGTTCTCTATGTGATTTTGTATATGAATAATGTAGAAGAATCTGCTGGATTGATGAACATTGTAGGGAAGTTGTTGATGAGTACACTGCTAATGTGAAGCTGATAAGTGAGAAAATCCTTAAGGCCATGGCAAGGTCATTAGATTTAGATGAGAGTAGCTTTCTTAACCAGTATGGTGAGCGAATTCAGCTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCAGCAATCACCATTTTGTTGCAGGACAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCCCCGATCATTCCCGGCGCTCTTCTCGTCAATGTCGGGGATCAAGCAGAGGTAAAACATGCTGTAAAAACAAACATTTAATTTTTAAATCACTGTCAAACATGTTCGTAAACATACTCGTATTGTGACAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCACAGGGTATTGACAAACTCAGAGAGGGAGAGGATATCTTTGGCAGTTTTTTACCTTCCAGATTCAGACAAAGAAATTGAACCATTAGAGAAGCTGATTAATGAAACTCAGCCAAGGTTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAAATTTGTTCTATGATATTTCTACCTCTCATTTTCTACCTGCCATTTTCATGGTTGATTGAAGTCATTTACACAACAAAAAAGCTTATAATATCTATGTTTTAGATCATTTTTGTCCTTGTGATTAGGATTTGATTCCATATTAATCCATCTAATATGAAATGTCCAATGAACTTTAAATAAACCTAGTTTGAAGTTAGTTCTATCAAAA

mRNA sequence

ATTTTTTTTTCTTTGAAATTTTAGAGTTATTTCTGCAACGACTTTGTTTATATAATAACAATAAGTATATATTTTTAACACTTTTAAGTTTATGAATATTTTTTAAAACTTTAGAAATTTCAGCACTCTAACATTTTTCATCCAAATCTAAACGTAGGAGAGCAGCGGTGTCGATGAAACCTGATCCATCGTAACAGAGGATGTCTCTTTTTTCCCCCTGCATTTCATTTGTTCCAGAGGCGACGAAATAAAGCCGTCGTTTCTTGACCACCAGCCATGGCCGGAACTAATCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTGAACGCTCCGTTAATGGCAGCTCCTGTAATCGATATCGGTCTCTTCTCATCCGGACCGGAACTGGAGAAACTCCGCCATGGACTTCAATCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGTCAACTGAATTTCTTGATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGGAAGAGAAACTGAAATACTCAAGAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAGACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATGTCCACAACAAAGCCGTCGTTTTAAGTATTGGCCAACAAATCCTGAAAGATTTAGGGAAGTTGTTGATGAGTACACTGCTAATGTGAAGCTGATAAGTGAGAAAATCCTTAAGGCCATGGCAAGGTCATTAGATTTAGATGAGAGTAGCTTTCTTAACCAGTATGGTGAGCGAATTCAGCTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCAGCAATCACCATTTTGTTGCAGGACAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCCCCGATCATTCCCGGCGCTCTTCTCGTCAATGTCGGGGATCAAGCAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCACAGGGTATTGACAAACTCAGAGAGGGAGAGGATATCTTTGGCAGTTTTTTACCTTCCAGATTCAGACAAAGAAATTGAACCATTAGAGAAGCTGATTAATGAAACTCAGCCAAGGTTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAAATTTGTTCTATGATATTTCTACCTCTCATTTTCTACCTGCCATTTTCATGGTTGATTGAAGTCATTTACACAACAAAAAAGCTTATAATATCTATGTTTTAGATCATTTTTGTCCTTGTGATTAGGATTTGATTCCATATTAATCCATCTAATATGAAATGTCCAATGAACTTTAAATAAACCTAGTTTGAAGTTAGTTCTATCAAAA

Coding sequence (CDS)

ATGGCCGGAACTAATCCTTCCGGCACTGTCCAAGATGTAGCTTCTAAAGGGGAAGTCCCGGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCTGAACGCTCCGTTAATGGCAGCTCCTGTAATCGATATCGGTCTCTTCTCATCCGGACCGGAACTGGAGAAACTCCGCCATGGACTTCAATCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGTCAACTGAATTTCTTGATGAAGTTCGTCAATTAGCGAAACAGTTCTTTGGTCTTCCAATGGAAGAGAAACTGAAATACTCAAGAGAAGATGAAATGGAAGGATATGGCAATGACATGGTTCTTTCAGACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATGTCCACAACAAAGCCGTCGTTTTAAGTATTGGCCAACAAATCCTGAAAGATTTAGGGAAGTTGTTGATGAGTACACTGCTAATGTGAAGCTGATAAGTGAGAAAATCCTTAAGGCCATGGCAAGGTCATTAGATTTAGATGAGAGTAGCTTTCTTAACCAGTATGGTGAGCGAATTCAGCTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCAGCAATCACCATTTTGTTGCAGGACAAGGAAGTAGAAGGTCTGCAGTTCATGAAAGGTAACGAGTGGTTCAATGCCCCGATCATTCCCGGCGCTCTTCTCGTCAATGTCGGGGATCAAGCAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCACAGGGTATTGACAAACTCAGAGAGGGAGAGGATATCTTTGGCAGTTTTTTACCTTCCAGATTCAGACAAAGAAATTGAACCATTAGAGAAGCTGATTAATGAAACTCAGCCAAGGTTGTACAAGACTGTCAAGAACTTTGTTGGGCTTTACTTTGAGTACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAA

Protein sequence

MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFSSGPELEKLRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDEMEGYGNDMVLSDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI
Homology
BLAST of Bhi02G000754 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 9.0e-110
Identity = 190/341 (55.72%), Postives = 247/341 (72.43%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRG---ALNA--PLMAAPVIDIGLFSSG-----PELEKL 68
           TVQ+V + GE +PERY+H  +  G    LNA  P M  P ID+ L  S       EL KL
Sbjct: 8   TVQEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVLS 128
              L +WG  Q +NHG++  FLD++ +L K+FF LP EEK K +RE D ++GYGNDM+L 
Sbjct: 68  HSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILW 127

Query: 129 DQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLD 188
           D Q+LDW DRLY+T  P+  R+  +WP  P  FRE + EYT   +++ E+  KAMARSL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLE 187

Query: 189 LDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+SFL+ YGE   LD RFN YP C +PD V+GVKPHADGSAIT+LL DK+V GLQF K
Sbjct: 188 LEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQK 247

Query: 249 GNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEI 308
             +W+ API+P  +L+NVGDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P +DKEI
Sbjct: 248 DGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADKEI 307

Query: 309 EPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           +P+ +L++E +PRLYKTVK +V LYF+YYQQG+RP+EAA I
Sbjct: 308 QPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

BLAST of Bhi02G000754 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.0e-105
Identity = 183/341 (53.67%), Postives = 244/341 (71.55%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRG---ALN--APLMAAPVIDIG-LFSSG----PELEKL 68
           TVQ+V + G+ +PERY+H  +  G    LN   P M  P ID+  LFSS      E++KL
Sbjct: 8   TVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVLS 128
              L +WG  Q +NHG++  FLD++ +L KQFF LP EEK K +RE   ++GYGNDM+LS
Sbjct: 68  HSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILS 127

Query: 129 DQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLD 188
           D Q+LDW DRL+LT  P+  R+ K+WP  P  F E +DEYT   +++ EK  KAMARSL+
Sbjct: 128 DNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLE 187

Query: 189 LDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+ FL  YGE   +++RFNF+P C  PD V+G+KPHADGSAIT+LL DK+VEGLQF+K
Sbjct: 188 LEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLK 247

Query: 249 GNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEI 308
             +W+ API+P  +L+ +GDQ EI SNGI+KSPVHRV+TN E+ERIS+A F +P  DKEI
Sbjct: 248 DGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDKEI 307

Query: 309 EPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
            P + L+ E +PRLYKTV  +V L+++YYQQG+R +EAA I
Sbjct: 308 HPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

BLAST of Bhi02G000754 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 4.9e-100
Identity = 174/342 (50.88%), Postives = 233/342 (68.13%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKESDRGALNAPL------MAAPVIDIG-LFSSG----PELEK 68
           TVQ+V + GE +PERY++  +  G  + P       M   +ID+  LFSS      EL K
Sbjct: 8   TVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSK 67

Query: 69  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVL 128
           L   + +WG  Q +NHG+S   LD++ +L KQFF LP +EK KY+RE    +G+GNDM+L
Sbjct: 68  LHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMIL 127

Query: 129 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 188
           SD Q+LDW DRLYL   P+  R+ K+WP NP  FRE + EYT   +L+ EK  KA+ARSL
Sbjct: 128 SDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSL 187

Query: 189 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 248
           +L+++ FL  +GE   L+ RFN YP C  PD VLG+KPH+DGSA T++L DK VEGLQF+
Sbjct: 188 ELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFL 247

Query: 249 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 308
           K  +W+ A I+P  +L+NVGD  E+ SNGI+KSPVHRV+ N ++ERI +A F   D DKE
Sbjct: 248 KDGKWYKASILPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADEDKE 307

Query: 309 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           I+PL  L++E +PRLYK VK     +F+YYQQG+RP+EAA I
Sbjct: 308 IQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of Bhi02G000754 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.2e-99
Identity = 178/342 (52.05%), Postives = 237/342 (69.30%), Query Frame = 0

Query: 9   TVQDVASKGE-VPERYIHKES--DRGA-LNA--PLMAAPVIDIGLFSS-----GPELEKL 68
           TVQ+V + GE +PERY+   +  D G  LNA  P+M  P ID+ L  S       EL KL
Sbjct: 8   TVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKL 67

Query: 69  RHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVLS 128
              L +WG  Q INHG++   LD++ +L K+F  LP EEK KY+RE   ++GYGNDM+L 
Sbjct: 68  HSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILW 127

Query: 129 DQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLD 188
           D Q+LDW DRLY+T  P+  R+ K+WP  P  FRE + EYT    L+  ++ KAMA SL+
Sbjct: 128 DDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLE 187

Query: 189 LDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 248
           L+E+ FL+  GE   +D RFN YP C  PD V+GV+PHAD SA T+LL DK VEGLQF+K
Sbjct: 188 LEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLK 247

Query: 249 GNEWFNAPIIPG-ALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 308
             +W+ AP++    +L+NVGDQ EI SNGI+KSPVHRV+TN+E+ERIS+A F +P +DKE
Sbjct: 248 DGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADKE 307

Query: 309 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           I+P++ L++E +PRLYK VKN+V L  +YY QGQRP+ A+ I
Sbjct: 308 IQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPIAASLI 349

BLAST of Bhi02G000754 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.7e-69
Identity = 136/354 (38.42%), Postives = 208/354 (58.76%), Query Frame = 0

Query: 8   GTVQDV-----ASKGEVPERYIHKESDRGALNAPLMA------APVIDIGLFSSGP---- 67
           G + DV     +   +VPER+I +E +RG + + L         PVID+   S       
Sbjct: 12  GKIDDVQELIKSKPNKVPERFIREEYERGVVVSSLKTHHLHHQIPVIDLSKLSKPDNDDF 71

Query: 68  --ELEKLRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGY 127
             E+ KL    + WG FQ INHG+  E ++++ ++A +FF +P+EEK KY  E   ++GY
Sbjct: 72  FFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEKKKYPMEPGTVQGY 131

Query: 128 GNDMVLSDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILK 187
           G   + S+ Q LDW +   L V P Q R  K WP+ P RF E ++ Y+  ++ + +++LK
Sbjct: 132 GQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELCKRLLK 191

Query: 188 AMARSLDLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK-E 247
            +A SL L E  F   +GE +Q   R N+YP C +PDLVLG+ PH+DGSA+T+L Q K  
Sbjct: 192 YIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVLGLSPHSDGSALTVLQQSKNS 251

Query: 248 VEGLQFMKGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFY 307
             GLQ +K N W     +P AL++N+GD  E+ SNG +KS  HR +TN E+ER+++  FY
Sbjct: 252 CVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNREKERLTIVTFY 311

Query: 308 LPDSDKEIEPLEKLI-NETQPRLYKTVKNFVGLYFEYY----QQGQRPMEAARI 338
            P+ + EIEP+ +L+ +ET P  Y++  +  G Y  +Y     QG++ ++ A+I
Sbjct: 312 APNYEVEIEPMSELVDDETNPCKYRSYNH--GDYSYHYVSNKLQGKKSLDFAKI 362

BLAST of Bhi02G000754 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.8e-62
Identity = 120/320 (37.50%), Postives = 182/320 (56.88%), Query Frame = 0

Query: 6   PSGTVQDVASKG--EVPERYIHKESDRGALNAPLMAA----PVIDIGLFSSGPE-LEKLR 65
           P  +VQ ++  G   VP RY+     R   N     A    PV+D+      PE L  +R
Sbjct: 8   PIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLRLVR 67

Query: 66  HGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRE-DEMEGYGNDMVLSD 125
              + WG FQ +NHG++   ++ VR   ++FF LP+EEK KY+   D  EGYG+ + +  
Sbjct: 68  SACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVK 127

Query: 126 QQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDL 185
              LDW+D  +L   P   R    WP+ P + RE++++Y   V+ + E++ + ++ SL L
Sbjct: 128 DAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGL 187

Query: 186 DESSFLNQY--GERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 245
             +  +     G+++    R NFYP+C  P L LG+  H+D   ITILL D++V GLQ  
Sbjct: 188 KPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQVR 247

Query: 246 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 305
           +G+ W     +P AL+VN+GDQ +I SNGI+KS  H+V+ NS  ER+SLA FY P SD  
Sbjct: 248 RGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIP 307

Query: 306 IEPLEKLINETQPRLYKTVK 316
           + P+E+L+   +P LYK ++
Sbjct: 308 VGPIEELVTANRPALYKPIR 327

BLAST of Bhi02G000754 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.8e-58
Identity = 120/319 (37.62%), Postives = 176/319 (55.17%), Query Frame = 0

Query: 6   PSGTVQDVASK--GEVPERYIHKESDRGALNAPLMAA---PVIDI-GLF-----SSGPEL 65
           P   VQ +A      +P+RYI   S R         A   P+ID+ GLF     S    +
Sbjct: 23  PIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIM 82

Query: 66  EKLRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDE-MEGYGNDM 125
            ++    + WG FQ +NHG+  E +D  R+  ++FF +P+  K  YS      EGYG+ +
Sbjct: 83  ARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRL 142

Query: 126 VLSDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMAR 185
            +     LDW+D  +L + P   + F  WP+ P   REV+DEY   +  +S +I++ ++ 
Sbjct: 143 GVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLST 202

Query: 186 SLDLDESSFLNQY-GERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGL 245
           +L L E  F   + GE I    R N+YP+C  P+L LG+ PH+D   +TILL D +V GL
Sbjct: 203 NLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGL 262

Query: 246 QFMKGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDS 305
           Q  K + W      P A +VN+GDQ +I SN  +KS  HRV+ NS++ER+SLA FY P S
Sbjct: 263 QVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKS 322

Query: 306 DKEIEPLEKLINETQPRLY 312
           D  I+PL++L++   P LY
Sbjct: 323 DIPIQPLQELVSTHNPPLY 341

BLAST of Bhi02G000754 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 3.0e-57
Identity = 115/330 (34.85%), Postives = 188/330 (56.97%), Query Frame = 0

Query: 19  VPERYIHKESDRGALNAPL---MAAPVIDIGLFSSG----PELEKLRHGLQSWGCFQAIN 78
           VP RY+  + D+  ++      +  P+ID+    S      E+EKL    + WG FQ +N
Sbjct: 29  VPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVN 88

Query: 79  HGMSTEFLDEVRQLAKQFFGLPMEEKLKY-SREDEMEGYGNDMVLSDQQILDWTDRLYLT 138
           HG+ + FLD+V+   + FF LPMEEK K+  R DE+EG+G   V+S+ Q LDW D  + T
Sbjct: 89  HGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHT 148

Query: 139 VCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDESSFLNQYGERI 198
           V P + R+   +P  P  FR+ ++ Y++ V+ +++ ++  MAR+L++        + +  
Sbjct: 149 VQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVD 208

Query: 199 QLDA-RFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAPIIPGA 258
            + + R N+YP C  PD V+G+ PH+D   +T+L+Q  +VEGLQ  K  +W     +P A
Sbjct: 209 SVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNA 268

Query: 259 LLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLEKLINETQPR 318
            +VN+GD  EI +NG ++S  HR + NSE+ER+S+A F+     KE+ P + L+   +  
Sbjct: 269 FIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVA 328

Query: 319 LYK--TVKNFVGLYFEYYQQGQRPMEAARI 338
            +K  T+K +    F     G+  ++A RI
Sbjct: 329 RFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of Bhi02G000754 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-57
Identity = 122/319 (38.24%), Postives = 172/319 (53.92%), Query Frame = 0

Query: 6   PSGTVQDVASKG--EVPERYIHKESDRGAL----NAPLMA---APVIDIGLFSSGPELEK 65
           P   VQ +A      +P+RYI   S R       + P +A    P+ID+    SG E +K
Sbjct: 52  PIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNEDDK 111

Query: 66  LR--HGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDE-MEGYGNDM 125
            R     + WG FQ INHG+  E +D  R+  K FF LP+E K  YS      EGYG+ +
Sbjct: 112 KRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYGSRL 171

Query: 126 VLSDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMAR 185
            +    ILDW D  YL   P   + F  WP+ P   RE+ DEY   +  +  +++  ++ 
Sbjct: 172 GVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTILSS 231

Query: 186 SLDLDESSFLNQY-GERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGL 245
           +L L        + GE +    R N+YP+C  P+L LG+ PH+D   +TILL D +V GL
Sbjct: 232 NLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQVVGL 291

Query: 246 QFMKGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDS 305
           Q   G+ W     +  A +VN+GDQ +I SN  +KS  HRV+ NSE+ER+SLA FY P S
Sbjct: 292 QVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFYNPKS 351

Query: 306 DKEIEPLEKLINETQPRLY 312
           D  I+P+++L+  T P LY
Sbjct: 352 DIPIQPMQQLVTSTMPPLY 370

BLAST of Bhi02G000754 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.3e-56
Identity = 117/329 (35.56%), Postives = 184/329 (55.93%), Query Frame = 0

Query: 19  VPERYIHKE-SDRGALN--APLMAAPVIDIGLF----SSGPELEKLRHGLQSWGCFQAIN 78
           +PERYI  E S    +N     MA P+ID+       SS  E  KLR   Q WG F  IN
Sbjct: 29  IPERYIRPEASSEEVINNYQGDMAIPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLLIN 88

Query: 79  HGMSTEFLDEVRQLAKQFFGLPMEEKLKYSR-EDEMEGYGNDMVLSDQQILDWTDRLYLT 138
           HG+  E +  +++    FF  P++ K +Y++  + +EGYG   V S+ Q LDW D LYL 
Sbjct: 89  HGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLYLH 148

Query: 139 VCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDESSFLNQYGERI 198
           V P  SR  ++WPT+P  FR+ +D Y++  K ++  + + MA+++     S L+ + E+ 
Sbjct: 149 VHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEEQP 208

Query: 199 QLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEWFNAPIIPGAL 258
           +   R  +YP CR  D V+G+ PH+D   +T+LL+   V+GLQ  K  +WF+     GAL
Sbjct: 209 R-GLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNGAL 268

Query: 259 LVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLEKLINETQPRL 318
           + N+GD  EI SNG F+S  HR + N  +ERIS A+F+ P  +  I PL + + + + + 
Sbjct: 269 IANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDGKVK- 328

Query: 319 YKTVK--NFVGLYFEYYQQGQRPMEAARI 338
           Y+++   +F+   F     G+  +E  ++
Sbjct: 329 YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of Bhi02G000754 vs. NCBI nr
Match: XP_038878494.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 692.2 bits (1785), Expect = 2.2e-195
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFSSGPELEKLRHG 60
           MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFSSGPELEKLRHG
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFSSGPELEKLRHG 60

Query: 61  LQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDEMEGYGNDMVLSDQQI 120
           LQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDEMEGYGNDMVLSDQQI
Sbjct: 61  LQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSREDEMEGYGNDMVLSDQQI 120

Query: 121 LDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDES 180
           LDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDES
Sbjct: 121 LDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSLDLDES 180

Query: 181 SFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEW 240
           SFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEW
Sbjct: 181 SFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKGNEW 240

Query: 241 FNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLE 300
           FNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLE
Sbjct: 241 FNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKEIEPLE 300

Query: 301 KLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           KLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 301 KLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 337

BLAST of Bhi02G000754 vs. NCBI nr
Match: KAA0050082.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 8.8e-176
Identity = 306/342 (89.47%), Postives = 323/342 (94.44%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWPTNP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+P ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Bhi02G000754 vs. NCBI nr
Match: TYK10346.1 (protein SRG1-like [Cucumis melo var. makuwa])

HSP 1 Score: 620.5 bits (1599), Expect = 8.3e-174
Identity = 303/342 (88.60%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWP NP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+  ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Bhi02G000754 vs. NCBI nr
Match: XP_008465535.1 (PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 302/342 (88.30%), Postives = 322/342 (94.15%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWPTNP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL+QYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK+VEGLQFM
Sbjct: 200 DLDESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+  ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Bhi02G000754 vs. NCBI nr
Match: XP_011655688.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027.1 hypothetical protein Csa_019640 [Cucumis sativus])

HSP 1 Score: 619.0 bits (1595), Expect = 2.4e-173
Identity = 303/342 (88.60%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVR+L KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 61  LRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEEKLKYLKEELEMEGYGNDMIL 120

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWPTN +RFREV+DEYT NVKLISEKI KAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDEYTTNVKLISEKIFKAMARSL 180

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL QYGE+I+LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 181 DLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEWFNAPI+P ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPD++KE
Sbjct: 241 KDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDAEKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LI+ETQPRLYK+VKNFVGL FEYYQQGQRPMEAARI
Sbjct: 301 IEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPMEAARI 342

BLAST of Bhi02G000754 vs. ExPASy TrEMBL
Match: A0A5A7U2M0 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00660 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.3e-176
Identity = 306/342 (89.47%), Postives = 323/342 (94.44%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWPTNP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+P ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQGQRPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI 361

BLAST of Bhi02G000754 vs. ExPASy TrEMBL
Match: A0A5D3CIZ6 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00230 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 4.0e-174
Identity = 303/342 (88.60%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWP NP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPINPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFLNQYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM
Sbjct: 200 DLDESSFLNQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+  ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Bhi02G000754 vs. ExPASy TrEMBL
Match: A0A1S3CP33 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.9e-174
Identity = 302/342 (88.30%), Postives = 322/342 (94.15%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MA TNPSGTVQDVASKGEVPERYIHKESDRGA NAPLMAAPVIDI L S    SGPELEK
Sbjct: 20  MASTNPSGTVQDVASKGEVPERYIHKESDRGARNAPLMAAPVIDIALLSSSSKSGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQAINHGM++E+LDEVRQL KQFFGL MEEKLKY +E+ EMEGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLSMEEKLKYLKEEHEMEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P QSRRFKYWPTNP+RFREVV EYT NVKLISEKILKAMARSL
Sbjct: 140 SNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREVVGEYTTNVKLISEKILKAMARSL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DLDESSFL+QYGE+++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDK+VEGLQFM
Sbjct: 200 DLDESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKKVEGLQFM 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
           K NEW+NAPI+  ALLVNVGDQ EITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 260 KDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLE+LINE+QPRLYK+VKNFVGLYFEYYQQG+RPMEAARI
Sbjct: 320 IEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPMEAARI 361

BLAST of Bhi02G000754 vs. ExPASy TrEMBL
Match: A0A6J1JKD6 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485215 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 298/342 (87.13%), Postives = 322/342 (94.15%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIH ESDRGAL+APLMAAPVID+ L S    SGPELEK
Sbjct: 1   MAGTNPSGTVQDVASKGEVPERYIHIESDRGALDAPLMAAPVIDMALLSSPSKSGPELEK 60

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSRED-EMEGYGNDMVL 120
           LRHGLQSWGCFQ +NHGMS EFLDEVR+LAKQFF LPMEEK KYSRE+ E+EGYGNDM+L
Sbjct: 61  LRHGLQSWGCFQVVNHGMSAEFLDEVRRLAKQFFDLPMEEKSKYSREENEIEGYGNDMIL 120

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P++S RFKYWPTNPERFREV+ EYTANVKL+SEKILKAMA SL
Sbjct: 121 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMAISL 180

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DL+E SF+ QYGE ++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD+EVEGLQF+
Sbjct: 181 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 240

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
            GNEWFNAPI+PGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDS+KE
Sbjct: 241 NGNEWFNAPIVPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSEKE 300

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLEKLI+ET+PRLYK+VKNFVGLYF+YYQQGQRPMEAARI
Sbjct: 301 IEPLEKLIDETRPRLYKSVKNFVGLYFQYYQQGQRPMEAARI 342

BLAST of Bhi02G000754 vs. ExPASy TrEMBL
Match: A0A6J1H9T6 (protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.9e-172
Identity = 296/342 (86.55%), Postives = 320/342 (93.57%), Query Frame = 0

Query: 1   MAGTNPSGTVQDVASKGEVPERYIHKESDRGALNAPLMAAPVIDIGLFS----SGPELEK 60
           MAGTNPSGTVQDVASKGEVPERYIHKESDRGA +APLMAAPVID+ L S    SGPELEK
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  LRHGLQSWGCFQAINHGMSTEFLDEVRQLAKQFFGLPMEEKLKYSR-EDEMEGYGNDMVL 120
           LRHGLQSWGCFQ +NHGMS EFLDE+R+LAKQFF LPMEEK KYSR EDE+EGYGNDM+L
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 SDQQILDWTDRLYLTVCPQQSRRFKYWPTNPERFREVVDEYTANVKLISEKILKAMARSL 180
           S+QQILDWTDRLYLTV P++S RFKYWPTNPERFR V+ EYTANVKL+SEKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDESSFLNQYGERIQLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFM 240
           DL+E SF+ QYGE ++LDARFNFYPRCRNPDLVLGVKPHADGSAITILLQD+EVEGLQF+
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWFNAPIIPGALLVNVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDSDKE 300
            GNEWFNAPI+PGALL+NVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPD +KE
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 IEPLEKLINETQPRLYKTVKNFVGLYFEYYQQGQRPMEAARI 338
           IEPLEKLI+ET+PRLYKTVKNFVGLYF+YYQQGQRPMEAARI
Sbjct: 320 IEPLEKLIDETRPRLYKTVKNFVGLYFQYYQQGQRPMEAARI 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G20400.19.0e-11055.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G49390.13.0e-10553.672-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G54000.14.9e-10050.882-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G20550.14.2e-9952.052-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G21420.17.7e-6938.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
Match NameE-valueIdentityDescription
O804496.8e-6237.50Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q9FFF67.8e-5837.62Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q392243.0e-5734.85Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1[more]
Q9SRM33.9e-5738.24Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1[more]
Q94LP43.3e-5635.562-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947... [more]
Match NameE-valueIdentityDescription
XP_038878494.12.2e-195100.00probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida][more]
KAA0050082.18.8e-17689.47protein SRG1-like [Cucumis melo var. makuwa][more]
TYK10346.18.3e-17488.60protein SRG1-like [Cucumis melo var. makuwa][more]
XP_008465535.11.8e-17388.30PREDICTED: protein SRG1-like [Cucumis melo] >XP_016903407.1 PREDICTED: protein S... [more]
XP_011655688.22.4e-17388.60probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653027.... [more]
Match NameE-valueIdentityDescription
A0A5A7U2M04.3e-17689.47Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00... [more]
A0A5D3CIZ64.0e-17488.60Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00... [more]
A0A1S3CP338.9e-17488.30protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503160 PE=3 SV=1[more]
A0A6J1JKD61.3e-17287.13probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 OS=Cucurbita maxima... [more]
A0A6J1H9T64.9e-17286.55protein SRG1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461440 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 41..143
e-value: 1.9E-22
score: 80.2
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 196..289
e-value: 1.1E-21
score: 77.3
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 9..327
e-value: 7.4E-94
score: 316.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 9..330
NoneNo IPR availablePANTHERPTHR47991:SF632-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 9..330
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 14..315
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 190..290
score: 12.442497

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000754Bhi02M000754mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding