Bhi02G000693 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000693
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionomega-6 fatty acid desaturase, chloroplastic
Locationchr2: 18649089 .. 18653531 (-)
RNA-Seq ExpressionBhi02G000693
SyntenyBhi02G000693
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTGTATTTGCTCGCCATTTTCCTGTGGGGGCAGAAAAATGAGCGATAACAACGCCGCCTTGAACAATAGTGGCGTAAGCTCGAAAGGGGCCACCCCTTTCAGAGAATTCTATCCACATCTTACAGAACGATTGGCAAAGGATTTATACCCAATTTCCCATTTCAAATAGCAAACTTCCATACTTAAGGGGATACCCATTAAGAAATTTCCCCCTTTCCCCTTCTATTACCTCTTATTCGAAGAGCCCAAGCAAGCTAAGGGGGCCTTCTTAACTGGGATTTCTGCAATTCTGCACATAATGGCTTGCAGACTTGCAGATTCCATGGTTCTTTTCAATGTACGCTTCCCTTCTTTTTAATCTTACCCTTGTGTTCTTCTTTTCTCTTATTGTTTTGGTTTGATTGTCAGGGCCCCCATCAGAATTTAGCTCGAACCCAGAAAATTGCATCCCATTGTTCGCCAGGTATGTTTTAGTATCTTCCTTTTGGAGAATTATTTGAAGTTGTTTAAGAATTGATAATTGATTGATAGACTAAATTGTCTAGCAGTTGTGTGATACGGTGTGATACAGTGTGATGACAGTTCAGAGTATATCCATGGTAGATTCAATTCCATTTACTTTGTCACTCCAGAAGGAACCTCGCATGTTGCGGACATGGTTTCTGTAATTAAATATTGAGTAAAATTTGAAATTAGATAGAAGTTAGAGTCCTCAACAAATTTCAAGTACATTGTTACTTTTTCATATCCTCATTCAACTCAAGCCTGAATTTTATATACCATAAAATGAATTAATCGACAAGGAACCAACCAACATAGCTTAGATTTTGCATTTGTTTCTTTATTCTCAAAATGCCAACATAGCCTAGATTGTGCATTTGTTTCTTTATTCTCAAAATGGGTTTTTCTAAGGTTAGCATTTTTTTCCCCTTTTGCTGTCTCTTTGAGCATAAATAACTTGGATTTTCCATTGCCAATTTGGACGGTTCAAACATACTTATTCCGATAGACAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAGAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATATTGTTGAGTCCCTTCCTAAAGAGGTACTTCATATTGTTGCAACATACTGTTGTTTAACATCTGCCCTTGAGGCCGTTGCATTTTCATTTCTTCTGTCAAATGAAATAATTTTGATGATACTGTAATTCATGTTTATAGTTTCTTACATAGCCTAGTTAGCTTATGTTCATGTTTCTTTTACTCATTGTATCTTATTCTTTTCATTTTTTTTAAAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCGTCATGATTGCAAAAGTTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGGTTAGTCTTATTCAAATATTCTTTCTGGTTGCTTCCCTTTCCCTTTCTTTTCCTCTCAACCTTGTCAGACTTGCAACTCTGGCCACTGAAGTTACTTTCCTCCTTAAGTTCTTTGTTATAGGTCACGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGTATTAGTAATCTCTCCTCTGTCTCTATCTCTCTTTGAAATACTTAATTTAGCTCTATCTTCCTGCATAAGCATTGTTCAATATTGTTCTGTCCGAAATGTCTTATTGAGGTTGGATAACATCTAGTTAAGCAATGCAAAAGCCTTTATCAGTAATATTGTGCAGAACAATATGAAGGGATATTAATGTGTTATAAAATTATAATCCATACCTTGATGGTGCTAGGTTTGTCCACATGACGTCTTATTTACTTATCACTGTTAGTTCTCCCTTGTATCTTGGGATCAAGTTAATTGAATTTCTCCACATGTTTTGCATTTTTCCTAGAGTCAAATATGGTTGTGTTAATTAAGCTTGAAATACTGCATGTATGAAGAAATAGGAATCCATATTTTATAAGTTTGGCTTGTTACTATTTATGCCTTTCATCTTGTAGTTTTAGCTAACCTTCCATTTCTGTTCTTATCCACGTATTATAGGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTAAGTTAACCTTGGATGGTTCTTTTGCTAGAATAATTTGACTTTATATGAAGATATCTTGTATGTGATTTGACCAAATTCTCTCTTTCTTTCATTAATATGCACCAATTTGTTCTTATGCATAATAGGTTGTTGTGGCACTTTGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGGTAACATCATTTGCTTGCCTCTTCTTATTACAGTTTCTCCTATAGTCCTATGATCAATTCAATTGATGTTCTGCGTTGGATAGTGATTTGTCATGGTTCTCTATGATGTAACAATGGATTAACAAACTAGTTAACAATCAGAATCAGAGGTTATATGGCAGGGTAGACTAATAAAAATGGACCTTGTAGCTTAGACTACAGTATTCAAGTATCTTTTCATATTGTTGGAACATTAATCTTAATGAAGCTTATATCTTGCACTGATATTCCTGAAGCAAATAAAAGAATGCTTAATACCGTGTTTTCAAGATCATGAGACAATGATATGGTGTTTATGCCACTGTTCTTCATTCATAGAGGGACTATTTTATTGTTCTTATTCTGCATAACTTGAAATTGTCCCAGATAAAATTTGTTGGTTTTACTTATGTAAATTGAGCTTCTATTTAAATTACCTTTTGTTTCTTTCTTAGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAGTGGTAACTGATCATTCATTTCAGTGTCTGTTTTTCCTTCTCTTTTCTTTTTTCTCGCTTAATTCGACTCTAAGCTGATAGAAAGGCGTATTAGAAAAAGATTCAGCACTATTCGTGAGATCCTCTAGTCTTGAAATCTAGATAATACATGTGAATTAAGATTTATCACACAAGTCACATTGACTTGGCTTCTAAGTTTATCTTCTCCCCTCTACAGGATTGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATTCCGAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGGTAATGGGGTAAACCGAATGCATGTGTGCGTTTTCTTTTCAAACAGTTCTTGCAAAATCTAACGATTGCCTTGCTTAATGCATATATTTTCCCCTACTGATGCTAATAATCTAGTTCTTTTCTTCCATCTTTGTTTTCCCTGCAACACATTTATAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTTCTCAAGAAAGTTATGCCTGATCTTGCTTGATTGATCTTACCTCAACCTTCAGGAACAACACAGACACTTGCTTCTTTCTTTCGTTGTGCAATGTTTTTCTCCACAAATTTTGAAGAACGAATGGAAGGAAACTTGGTAGGACGCCATATATTGAGTTAAAGAATTTCTCTATTTCATTTTCCAATCTCTCGCTCTCCCATTTTCTCGTGAGGGAACATTTTTTTCTCCTCAAATAGCAATAGTCTGTAACCTTTTCTTTTGTTCTTTTAACGTAGTTGTGATCTAAAGAGGAAGAACAAACACTTTGATACTTTATGTTGCCACTTTATAAAAAGGTGTAATGGAAACACTCTCTTTATCAAAGACACCGACTGACAATACAAGTACAACACAAC

mRNA sequence

CTTTTGTATTTGCTCGCCATTTTCCTGTGGGGGCAGAAAAATGAGCGATAACAACGCCGCCTTGAACAATAGTGGCGTAAGCTCGAAAGGGGCCACCCCTTTCAGAGAATTCTATCCACATCTTACAGAACGATTGGCAAAGGATTTATACCCAATTTCCCATTTCAAATAGCAAACTTCCATACTTAAGGGGATACCCATTAAGAAATTTCCCCCTTTCCCCTTCTATTACCTCTTATTCGAAGAGCCCAAGCAAGCTAAGGGGGCCTTCTTAACTGGGATTTCTGCAATTCTGCACATAATGGCTTGCAGACTTGCAGATTCCATGGTTCTTTTCAATGGCCCCCATCAGAATTTAGCTCGAACCCAGAAAATTGCATCCCATTGTTCGCCAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAGAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATATTGTTGAGTCCCTTCCTAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCGTCATGATTGCAAAAGTTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCACGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTTGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAGTGGATTGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATTCCGAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTTCTCAAGAAAGTTATGCCTGATCTTGCTTGATTGATCTTACCTCAACCTTCAGGAACAACACAGACACTTGCTTCTTTCTTTCGTTGTGCAATGTTTTTCTCCACAAATTTTGAAGAACGAATGGAAGGAAACTTGTTGTGATCTAAAGAGGAAGAACAAACACTTTGATACTTTATGTTGCCACTTTATAAAAAGGTGTAATGGAAACACTCTCTTTATCAAAGACACCGACTGACAATACAAGTACAACACAAC

Coding sequence (CDS)

ATGGCTTGCAGACTTGCAGATTCCATGGTTCTTTTCAATGGCCCCCATCAGAATTTAGCTCGAACCCAGAAAATTGCATCCCATTGTTCGCCAGGTATATTGCAAGTGAAGGGGGAAATATTATTTCAGAAGAAAATCAAGCGACAGAACTATCTACTTCCTGTGAAGAGGGCTGCAATTTTACGGGCTGTTGCTGTTCCAGTTGCACCACCATCTTCTTCAGCAGATAGTGCTGAATATAGAAAACAATTATCAGAAAGCTATGGATTCGAGCAAATTGGAGAACCTCTTCCTGATAATGTTAGGTTAAAAGATATTGTTGAGTCCCTTCCTAAAGAGGTGTTTGAGATCAATGATGTGAAAGCATGGAAATCTGTTTTAGTATCTGTAGCTTCCTACACATTAGGCCTCGTCATGATTGCAAAAGTTCCATGGTATCTACTACCTTTGGCTTGGGCATGGACTGGAACTGCAATAACAGGGTTCTTTGTTATAGGTCACGATTGTGCTCACAAATCATTTTCAAGGAACAAGTTAGTGGAAGACATTGTGGGTACTTTGGCTTTCATGCCTCTAATATACCCATATGAGCCTTGGCGGTTTAAGCATGATCAACATCATGCAAAGACAAACATGCTGGAAGAAGATACAGCTTGGCATCCTGTGTGGAAAGAGGAATTTGATTCAGCACCTCTTTTGCGTAAAGCAATCATATATGGTTATGGTCCATTTCGACCGTGGATGTCTATAGCTCATTGGTTGTTGTGGCACTTTGATTTGAAAAAGTTCAGACCAAACGAAGTGAAAAGAGTGAAGATAAGCTTAGCTTGTGTTTTTGCATTTATGTTTATTGGATGGCCATTGATCATCTACAAGACTGGGATTATTGGATGGGTCAAGTTCTGGTTAATGCCATGGCTGGGCTACCACTTTTGGATGAGCACTTTCACCATGGTCCATCACACAGCTCCTCATATACCGTTTAAGTCTTCAGAAGAGTGGAACGCAGCTCAAGCGCAGCTCAATGGAACAGTTCACTGTGCTTATCCTCAGTGGATTGAGATTCTCTGCCATGACATCAATGTGCACATTCCCCACCATATTTCTCCAAGAATTCCGAGTTACAACTTACGGGCAGCCCACAAGTCTCTTCAAGAAAACTGGGGAAAGCATCTGAATGAGGCTTCATGGAATTGGCGATTAATGAAGACGATCATGACGATATGCCATGTCTACAGTAAAGAGGAAAACTATGTCGCATTTGACCGACTCAATAAAGAAGATTCTTATCCCATTACATTTCTCAAGAAAGTTATGCCTGATCTTGCTTGA

Protein sequence

MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLNKEDSYPITFLKKVMPDLA
Homology
BLAST of Bhi02G000693 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 714.9 bits (1844), Expect = 3.9e-206
Identity = 336/447 (75.17%), Postives = 384/447 (85.91%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIA---SHCSPGILQVKG-EILFQKKIKR-QNYLLPV 60
           MA R+ADS+  F GP Q L R  K+A   +  SPG+  VK  ++L + +  R +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEV 120
           K R   ++AVA PVAPP  SADSAE R+QL+ESYGF QIGE LP+NV LKDI+++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 121 FEINDVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180
           FEI+D+KA KSVL+SV SYTLGL MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 181 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLR 240
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 241 KAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 300
           KAII+GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 301 IIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIE 360
           I+GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 361 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSK 420
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 421 EENYVAFDRLNKEDSYPITFLKKVMPD 442
           EENY+ FDRL  E+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of Bhi02G000693 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 129.8 bits (325), Expect = 5.4e-30
Identity = 98/344 (28.49%), Postives = 153/344 (44.48%), Query Frame = 0

Query: 94  GEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKVP----WYLLP 153
           G P P N  L DI  ++PK  +  N    WKS+   V    +   + A       W + P
Sbjct: 91  GAPPPFN--LADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWP 150

Query: 154 LAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAK 213
           L W   GT     FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H  HH  
Sbjct: 151 LYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQN 210

Query: 214 TNMLEEDTAWHPVWKEEFD-----------SAPLLRKAIIYGYGPFRPWMSIAHWLLWHF 273
              +E D +WHP+ ++ ++           + PL+  A  +      P    +H   +H 
Sbjct: 211 HGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH---YHP 270

Query: 274 DLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTM 333
           D   F P E K V  S AC  A   +    + +  G I  +K + +P+     W+   T 
Sbjct: 271 DSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWLDFVTY 330

Query: 334 VHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYN 393
           +HH      +P+   +EW+  +  L  T+   Y   I  + HDI  H+ HH+ P+IP Y+
Sbjct: 331 LHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQIPHYH 390

Query: 394 LRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 421
           L  A ++ +   GK+  E   +  L   ++ I     KE++YV+
Sbjct: 391 LVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of Bhi02G000693 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-29
Identity = 86/334 (25.75%), Postives = 159/334 (47.60%), Query Frame = 0

Query: 102 RLKDIVESLPKEVFEINDVKAWKSV---LVSVASYTLGLVMIAKVPWYLLPLAWAWTGTA 161
           ++ DI  ++PK  +  + +++   V   +++VA+  +  V +    W+L PL WA  GT 
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDS--WFLWPLYWAAQGTL 94

Query: 162 ITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTA 221
               FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH     +E D +
Sbjct: 95  FWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHGHVENDES 154

Query: 222 WHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHWLLW--------HFD--LKKFRPNEV 281
           W P+ +  +   P   + + Y      P ++   +L +        HF+     F P+E 
Sbjct: 155 WVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSSLFAPSER 214

Query: 282 KRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA--PHI 341
           K +  S  C ++ MF+    + +  G +  +K + +P++ +  W+   T +HH      +
Sbjct: 215 KLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKL 274

Query: 342 PFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQE 401
           P+   +EW+  +  L  T+   Y  +  I  HDI  H+ HH+ P+IP Y+L  A K+ + 
Sbjct: 275 PWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVDATKAAKH 334

Query: 402 NWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 421
             G++  E   +  +   ++       K+++YV+
Sbjct: 335 VLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of Bhi02G000693 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 126.7 bits (317), Expect = 4.6e-29
Identity = 94/313 (30.03%), Postives = 140/313 (44.73%), Query Frame = 0

Query: 94  GEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKV--PWYLLPLA 153
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 154 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 213
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 214 MLEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKF 273
            +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 274 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA 333
            P E K V  S AC  A   +    + +  G I  +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 334 --PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAH 393
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L  A 
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHLVEAT 383

Query: 394 KSLQENWGKHLNE 395
           ++ +   GK+  E
Sbjct: 384 EAAKPVLGKYYRE 390

BLAST of Bhi02G000693 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 122.5 bits (306), Expect = 8.7e-28
Identity = 90/296 (30.41%), Postives = 132/296 (44.59%), Query Frame = 0

Query: 94  GEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKV--PWYLLPLA 153
           G P P N  L DI  ++PK  +  N   +   V+  VA    GL  +A     W L PL 
Sbjct: 84  GAPPPFN--LADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLWPLY 143

Query: 154 WAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 213
           W   GT     FV+GHDC H SFS +  +  + G L    ++ PY  WR  H  HH    
Sbjct: 144 WFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHG 203

Query: 214 MLEEDTAWHP----VWKEEFDSAPLLRKAIIYGY--GPFRPWMSI--AHWLLWHFDLKKF 273
            +E D +WHP    ++K    +  + R  + +     PF  W          +H D   F
Sbjct: 204 HVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLF 263

Query: 274 RPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTA 333
            P E K V  S AC  A   +    + +  G I  +K + +P+  +  W+   T +HH  
Sbjct: 264 LPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVMWLDFVTYLHHHG 323

Query: 334 --PHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNL 378
               +P+   +EW+  +  L  T+   Y  WI  + HDI  H+ HH+ P+IP Y+L
Sbjct: 324 HEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLFPQIPHYHL 373

BLAST of Bhi02G000693 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 9.8e-210
Identity = 340/445 (76.40%), Postives = 385/445 (86.52%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIAS-HCSPGILQVKGEILFQKKIKRQNYLLPV-KRA 60
           MA R+ADS+  F GP Q L R  K+AS   SPG+  V+   L  K  +R  +L+P  KR 
Sbjct: 1   MASRIADSLFAFTGPQQCLPRAPKLASARLSPGVYAVRPIDLLLKGTRR-TFLVPAKKRI 60

Query: 61  AILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEIN 120
             ++AV VPVAPP  SAD+AE R+QL+ESYGF+QIG+ LPDNV LKDI+++LPKEVFEI+
Sbjct: 61  GCIKAVFVPVAPP--SADNAEDREQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEID 120

Query: 121 DVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNK 180
           DVKAWKSVL+SV SY LGL MIAK PWYLLPLAWAWTGTA+TGFFVIGHDCAHKSFS+NK
Sbjct: 121 DVKAWKSVLISVTSYALGLFMIAKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSKNK 180

Query: 181 LVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAII 240
           LVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEFDS+P+LRKAII
Sbjct: 181 LVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFDSSPVLRKAII 240

Query: 241 YGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGW 300
           +GYGP RPW+SIAHW+ WHF+L+KFRP+EV RVKISLACVFAFM +GWPLIIYK G++GW
Sbjct: 241 FGYGPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGWPLIIYKVGVLGW 300

Query: 301 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCH 360
           VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCH
Sbjct: 301 VKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCH 360

Query: 361 DINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENY 420
           DINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY KEENY
Sbjct: 361 DINVHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENY 420

Query: 421 VAFDRLNKEDSYPITFLKKVMPDLA 444
           + FDRL  E+S PITFLKK MPD A
Sbjct: 421 IPFDRLAPEESQPITFLKKAMPDYA 442

BLAST of Bhi02G000693 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 714.9 bits (1844), Expect = 5.6e-205
Identity = 336/447 (75.17%), Postives = 384/447 (85.91%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIA---SHCSPGILQVKG-EILFQKKIKR-QNYLLPV 60
           MA R+ADS+  F GP Q L R  K+A   +  SPG+  VK  ++L + +  R +  + PV
Sbjct: 1   MASRIADSLFAFTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPV 60

Query: 61  K-RAAILRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEV 120
           K R   ++AVA PVAPP  SADSAE R+QL+ESYGF QIGE LP+NV LKDI+++LPKEV
Sbjct: 61  KRRIGCIKAVAAPVAPP--SADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEV 120

Query: 121 FEINDVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180
           FEI+D+KA KSVL+SV SYTLGL MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSF
Sbjct: 121 FEIDDLKALKSVLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITGFFVIGHDCAHKSF 180

Query: 181 SRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLR 240
           S+NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S+P++R
Sbjct: 181 SKNKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMR 240

Query: 241 KAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTG 300
           KAII+GYGP RPW+SIAHW+ WHF+LKKFR +EV RVKISLACVFAFM +GWPLI+YK G
Sbjct: 241 KAIIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVG 300

Query: 301 IIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIE 360
           I+GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIE
Sbjct: 301 ILGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIE 360

Query: 361 ILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSK 420
           ILCHDINVHIPHHISPRIPSYNLRAAH+S+QENWGK+ N A+WNWRLMKTIMT+CHVY K
Sbjct: 361 ILCHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDK 420

Query: 421 EENYVAFDRLNKEDSYPITFLKKVMPD 442
           EENY+ FDRL  E+S PITFLKK MP+
Sbjct: 421 EENYIPFDRLAPEESQPITFLKKAMPN 445

BLAST of Bhi02G000693 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 6.1e-204
Identity = 324/423 (76.60%), Postives = 363/423 (85.82%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MAC LADS++LF G +Q     + IA+  SPGI  +    L QK+ +RQ   +   +  +
Sbjct: 1   MACTLADSLLLFKGSYQKPVLRRDIAARYSPGIFSLNSNGLIQKRFRRQRNFVTRNKVTV 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           + AVA+PV P  +  +SAEYRKQL+E YGF Q+GEPL D+V LKD++  LPKEVFEI+DV
Sbjct: 61  IHAVAIPVQP--APVESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVL+SV SY LGL MI+K PWYLLPLAW WTGTAITGFFVIGHDCAH+SFS NKLV
Sbjct: 121 KAWKSVLISVTSYALGLFMISKAPWYLLPLAWVWTGTAITGFFVIGHDCAHRSFSSNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHD+HHAKTNML EDTAWHPVWK+EF+S PLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFR WMSIAHWL+WHFDLKKFRP+EV RVKISLACVFAF+ IGWPLIIYKTGI+GW+K
Sbjct: 241 YGPFRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YP+WIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWG++LNEASWNWRLMKTIMT+C VY KE++   
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCC 420

Query: 421 FDR 424
             R
Sbjct: 421 LRR 421

BLAST of Bhi02G000693 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 4.1e-184
Identity = 292/390 (74.87%), Postives = 341/390 (87.44%), Query Frame = 0

Query: 56  KRAAILRAVAVPVAPPSS--SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKE 115
           +++ +++AVAVPVA PS+    D+ E+ KQL+E YGF+QIGEPLPD+V ++DI+ SLPK+
Sbjct: 58  RKSTLVQAVAVPVAQPSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPKQ 117

Query: 116 VFEINDVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 175
           VFEIND KAW +VL+SV SY LG+ MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKS
Sbjct: 118 VFEINDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKS 177

Query: 176 FSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLL 235
           FS+NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHH KTNML EDTAW P+ KE+ +S+P L
Sbjct: 178 FSKNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIMKEDIESSPGL 237

Query: 236 RKAIIYGYGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKT 295
           RKA+IY YGP R WMSIAHWL  HF+LK FR +EVKR  ISLA VFAFM IGWPLIIYKT
Sbjct: 238 RKALIYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMVIGWPLIIYKT 297

Query: 296 GIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWI 355
           GI+GW+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFKSS+EWNAAQAQL+GTVHC YP+WI
Sbjct: 298 GIVGWIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSGTVHCDYPRWI 357

Query: 356 EILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYS 415
           EILCHDI+VHIPHHISP+IPSYNLRAA++SL ENWG++LN+   NWRLM+TIMT CH+Y 
Sbjct: 358 EILCHDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMRTIMTTCHIYD 417

Query: 416 KEENYVAFDRLNKEDSYPITFLKKVMPDLA 444
           K+ NYV+F++   E+S PI+  K+VMPD A
Sbjct: 418 KDGNYVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of Bhi02G000693 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.2e-108
Identity = 177/332 (53.31%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 96  PLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWT 155
           P+ D ++L+DI+++LPKE FE    KAW SVL+++ +  +G + I  +PWY LP+ W WT
Sbjct: 20  PIAD-LKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWT 79

Query: 156 GTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEE 215
           GTA+TG FV+GHDC H+SF++ + V D+VG +AF PLIYP+  WR  HD HH  TN +E 
Sbjct: 80  GTALTGAFVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHTNKIEV 139

Query: 216 DTAWHPVWKEEFDSAPLLRKAIIYGY-GPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKIS 275
           D AW P   E F ++P + +       GPF    SI HW L HF L  F   +  +VK+S
Sbjct: 140 DNAWDPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRNKVKLS 199

Query: 276 LACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEW 335
           +A VF F  I +P +I  TG+ G+VKFWLMPWL YHFWMSTFT+VHHT P I F+ + +W
Sbjct: 200 IAVVFLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFRPAADW 259

Query: 336 NAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNE 395
           +AA+AQLNGTVHC YP+W+E+LCHDINVHIPHH+S  IPSYNLR AH SL+ENWG  L E
Sbjct: 260 SAAEAQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWGPFLYE 319

Query: 396 ASWNWRLMKTIMTICHVYSKEENYVAFDRLNK 427
            ++NW+LM+ I   CH+Y  E  Y  F  L K
Sbjct: 320 RTFNWQLMQQISGQCHLYDPEHGYRTFGSLKK 350

BLAST of Bhi02G000693 vs. NCBI nr
Match: XP_038880078.1 (omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 939.1 bits (2426), Expect = 1.4e-269
Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. NCBI nr
Match: XP_004146685.1 (omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65155.1 hypothetical protein Csa_019883 [Cucumis sativus])

HSP 1 Score: 925.2 bits (2390), Expect = 2.1e-265
Identity = 432/443 (97.52%), Postives = 437/443 (98.65%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DSM LFNGPH N ARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRA+AVP APPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. NCBI nr
Match: XP_008443861.1 (PREDICTED: omega-6 fatty acid desaturase, chloroplastic [Cucumis melo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.3e-264
Identity = 432/443 (97.52%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DSM LFNGPHQNLARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. NCBI nr
Match: XP_022930407.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 908.3 bits (2346), Expect = 2.6e-260
Identity = 424/443 (95.71%), Postives = 434/443 (97.97%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLADS+ LF GPHQN ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYTLGL+MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. NCBI nr
Match: XP_023529482.1 (omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.1 bits (2343), Expect = 5.8e-260
Identity = 423/443 (95.49%), Postives = 434/443 (97.97%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLADS+ LF GPHQN ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYTLGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVH+PHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHVPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLRKVMPDLA 443

BLAST of Bhi02G000693 vs. ExPASy TrEMBL
Match: A0A0A0LTB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G255170 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.0e-265
Identity = 432/443 (97.52%), Postives = 437/443 (98.65%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DSM LFNGPH N ARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLVDSMALFNGPHHNFARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRA+AVP APPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKD+VESLPKEVFEINDV
Sbjct: 61  LRAIAVPAAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDVVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVY+KEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYNKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. ExPASy TrEMBL
Match: A0A1S3B9S5 (omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487358 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 6.5e-265
Identity = 432/443 (97.52%), Postives = 438/443 (98.87%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRL DSM LFNGPHQNLARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAI
Sbjct: 1   MACRLVDSMALFNGPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASY+LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYSLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHH+SPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENYVA
Sbjct: 361 NVHIPHHVSPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRLNKEDSYPITFLKKVMPDLA
Sbjct: 421 FDRLNKEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. ExPASy TrEMBL
Match: A0A6J1EQE5 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436860 PE=4 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.3e-260
Identity = 424/443 (95.71%), Postives = 434/443 (97.97%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLADS+ LF GPHQN ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYTLGL+MIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLIMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPITFLKKVMPDLA
Sbjct: 421 FDRLKPEDSYPITFLKKVMPDLA 443

BLAST of Bhi02G000693 vs. ExPASy TrEMBL
Match: A0A6J1HLM6 (omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111465334 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.1e-259
Identity = 421/443 (95.03%), Postives = 433/443 (97.74%), Query Frame = 0

Query: 1   MACRLADSMVLFNGPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAI 60
           MACRLADS+ LF GPHQN ARTQKIASH +PGIL VKG+I+FQKKIKRQNYLLPVKRAAI
Sbjct: 1   MACRLADSLFLFTGPHQNYARTQKIASHNAPGILDVKGKIVFQKKIKRQNYLLPVKRAAI 60

Query: 61  LRAVAVPVAPPSSSADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDV 120
           LRAVAVPVAPPSSSAD AEYRKQLSESYGFEQIGEPLP+NVRLKDI+ESLPKEVFEINDV
Sbjct: 61  LRAVAVPVAPPSSSADGAEYRKQLSESYGFEQIGEPLPENVRLKDIIESLPKEVFEINDV 120

Query: 121 KAWKSVLVSVASYTLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180
           KAWKSVLVSVASYTLGLV+IAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV
Sbjct: 121 KAWKSVLVSVASYTLGLVVIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLV 180

Query: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYG 240
           EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML+EDTAWHPVWKEEFDSAPLLRKAI+YG
Sbjct: 181 EDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSAPLLRKAIVYG 240

Query: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300
           YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK
Sbjct: 241 YGPFRPWMSIAHWLLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVK 300

Query: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360
           FWLMPWLGYHFWMSTFTMVHHTAPHIPFK+SEEWNAAQAQLNGTVHCAYPQWIEILCHDI
Sbjct: 301 FWLMPWLGYHFWMSTFTMVHHTAPHIPFKASEEWNAAQAQLNGTVHCAYPQWIEILCHDI 360

Query: 361 NVHIPHHISPRIPSYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420
           NVHIPHHISPRIPSYNLRAAHKSL+ENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA
Sbjct: 361 NVHIPHHISPRIPSYNLRAAHKSLKENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVA 420

Query: 421 FDRLNKEDSYPITFLKKVMPDLA 444
           FDRL  EDSYPI FL+KVMPDLA
Sbjct: 421 FDRLKPEDSYPIKFLRKVMPDLA 443

BLAST of Bhi02G000693 vs. ExPASy TrEMBL
Match: A0A5D3BV55 (Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00370 PE=4 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.5e-258
Identity = 421/430 (97.91%), Postives = 427/430 (99.30%), Query Frame = 0

Query: 14  GPHQNLARTQKIASHCSPGILQVKGEILFQKKIKRQNYLLPVKRAAILRAVAVPVAPPSS 73
           GPHQNLARTQKIASHCSPG LQVKGEILFQKKIKRQNYLLPVK+AAILRAVAVPVAPPSS
Sbjct: 18  GPHQNLARTQKIASHCSPGTLQVKGEILFQKKIKRQNYLLPVKKAAILRAVAVPVAPPSS 77

Query: 74  SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASY 133
           SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASY
Sbjct: 78  SADSAEYRKQLSESYGFEQIGEPLPDNVRLKDIVESLPKEVFEINDVKAWKSVLVSVASY 137

Query: 134 TLGLVMIAKVPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI 193
           +LGLVMIAK PWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI
Sbjct: 138 SLGLVMIAKAPWYLLPLAWAWTGTAITGFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLI 197

Query: 194 YPYEPWRFKHDQHHAKTNMLEEDTAWHPVWKEEFDSAPLLRKAIIYGYGPFRPWMSIAHW 253
           YPYEPWRFKHDQHHAKTNMLEEDTAWHPV KEEFDSAP+LRKAIIYGYGPFRPWMSIAHW
Sbjct: 198 YPYEPWRFKHDQHHAKTNMLEEDTAWHPVLKEEFDSAPILRKAIIYGYGPFRPWMSIAHW 257

Query: 254 LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGIIGWVKFWLMPWLGYHFWM 313
           LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGI+GWVKFWLMPWLGYHFWM
Sbjct: 258 LLWHFDLKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKTGILGWVKFWLMPWLGYHFWM 317

Query: 314 STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHISPRIP 373
           STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHH+SPRIP
Sbjct: 318 STFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINVHIPHHVSPRIP 377

Query: 374 SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLNKEDSYPIT 433
           SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMT+CHVYSKEENYVAFDRLNKEDSYPIT
Sbjct: 378 SYNLRAAHKSLQENWGKHLNEASWNWRLMKTIMTVCHVYSKEENYVAFDRLNKEDSYPIT 437

Query: 434 FLKKVMPDLA 444
           FLKKVMPDLA
Sbjct: 438 FLKKVMPDLA 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G30950.13.9e-20675.17fatty acid desaturase 6 [more]
AT3G11170.15.4e-3028.49fatty acid desaturase 7 [more]
AT2G29980.11.2e-2925.75fatty acid desaturase 3 [more]
AT5G05580.14.6e-2930.03fatty acid desaturase 8 [more]
AT5G05580.28.7e-2830.41fatty acid desaturase 8 [more]
Match NameE-valueIdentityDescription
P486279.8e-21076.40Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1[more]
P463125.6e-20575.17Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P486286.1e-20476.60Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1[more]
P486294.1e-18474.87Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD... [more]
P203888.2e-10853.31Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ... [more]
Match NameE-valueIdentityDescription
XP_038880078.11.4e-269100.00omega-6 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
XP_004146685.12.1e-26597.52omega-6 fatty acid desaturase, chloroplastic isoform X1 [Cucumis sativus] >KGN65... [more]
XP_008443861.11.3e-26497.52PREDICTED: omega-6 fatty acid desaturase, chloroplastic [Cucumis melo][more]
XP_022930407.12.6e-26095.71omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita moschata][more]
XP_023529482.15.8e-26095.49omega-6 fatty acid desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LTB11.0e-26597.52FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G2551... [more]
A0A1S3B9S56.5e-26597.52omega-6 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1EQE51.3e-26095.71omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1HLM69.1e-25995.03omega-6 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A5D3BV554.5e-25897.91Omega-6 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 145..392
e-value: 5.0E-30
score: 105.2
NoneNo IPR availablePANTHERPTHR32100:SF67BNAC03G67820D PROTEINcoord: 1..443
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 1..443
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 121..377
e-value: 1.77937E-81
score: 248.68

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000693Bhi02M000693mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane