Bhi02G000648 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000648
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptioncinnamoyl-CoA reductase-like SNL6
Locationchr2: 17472730 .. 17474544 (+)
RNA-Seq ExpressionBhi02G000648
SyntenyBhi02G000648
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAAGATGTCAATTAAATCCCATTTTTCCACCATGATCATGATTATTAAGATTGCATATTCCAATCTCGGGGTGTACTAAACTTCCCCCCTTGAAAGCATATATCAGCAAACGACAAATTGCATTTCTCCCACTTTCCCCATTTTTCACCATCATTTCATGCCAAACAGTTCACCAAACATCTTCCTTACCTTACTCCCTCTTCTCCATCTCTATAAAATCAGCCCCATTCCACCTAAACACAGTACTACACGAAACAGAGACACCCAAAAATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTGGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAAAACAATGGCAAGTCATCTATCCTCTCTTCTTTTCCACTTCTTAATTCTCTGTCTGTATTTCAAACTTGTGGATTTGATATTTTCCAGGAAAGCCAGAGATTGAGAGGACTATTAGAGAGATGGGTAAAATGAAGAATGGGCTGGTGTTTTTTTCTGTGGATATCATGGACTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACTCCCCATGTGTTTGATGTAAGTCCAAAGAGAAATATAAATAAATAAATGAATAGAAAGATGGTCTGGATTTTGTGGGTTATTGTTTTTTTTGTGTTTTGTACAGGAAAAAATGGTAGATTTGGAAGTTAGAGGGACAATTAATGTTGTGGAGGCTTGTGCTCAGACAGATACTGTTAATAAGATTGTCTTCACTTCTTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGGTTGGGAAATTTCAAATCCCTCTTATTATCTTCATAAAGTTCCTCAAATAACAGATGCCTAATCAATCGATTTCATTGCAAATCAAGCTTTTCCTTTTCAAATCTAATGACCCTTTTTATATTATATCATCTCCCAGTTATGGTACCCTTTAGCAAAGACACTCTCTGAACAAGCTGCTTGGGCTTTAGCAGTGGACCGTAAGCTAAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAGGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGTAGGAGAAAGTCTTTCTTTTAGAAAAGTATTGTACAATTGTGGGAATTCTTTGTTTTGGCTAAGAAAATTTGAGGGGAAACAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAATTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTTGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGGTAAAAGCATTATTAGCCTTTTCAACAACAAAAATAGGCATTTTTTCAGTTTATATTTTGCCATATATTTGCATGTTATTGTTAATTGAAAGTTTGAAACTCAAATTAGGTTAGTTTATTGGTTGGTGAGTAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAATTGATCGAGGGCACGGCTTAATACTAAATTTTAAATTTTAAATAAATAAATACATAAATGGAACTTTTTCTTTTCCCTTTTGGTATGTATCTATTTTAGCCCTTAATTAATGGAAAAACTAATATTTAGCGTGCACGTAAATGGGAC

mRNA sequence

AATAAAGATGTCAATTAAATCCCATTTTTCCACCATGATCATGATTATTAAGATTGCATATTCCAATCTCGGGGTGTACTAAACTTCCCCCCTTGAAAGCATATATCAGCAAACGACAAATTGCATTTCTCCCACTTTCCCCATTTTTCACCATCATTTCATGCCAAACAGTTCACCAAACATCTTCCTTACCTTACTCCCTCTTCTCCATCTCTATAAAATCAGCCCCATTCCACCTAAACACAGTACTACACGAAACAGAGACACCCAAAAATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTGGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAAAACAATGGAAAGCCAGAGATTGAGAGGACTATTAGAGAGATGGGTAAAATGAAGAATGGGCTGGTGTTTTTTTCTGTGGATATCATGGACTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACTCCCCATGTGTTTGATGAAAAAATGGTAGATTTGGAAGTTAGAGGGACAATTAATGTTGTGGAGGCTTGTGCTCAGACAGATACTGTTAATAAGATTGTCTTCACTTCTTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTATGGTACCCTTTAGCAAAGACACTCTCTGAACAAGCTGCTTGGGCTTTAGCAGTGGACCGTAAGCTAAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAGGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAATTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTTGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAATTGATCGAGGGCACGGCTTAATACTAAATTTTAAATTTTAAATAAATAAATACATAAATGGAACTTTTTCTTTTCCCTTTTGGTATGTATCTATTTTAGCCCTTAATTAATGGAAAAACTAATATTTAGCGTGCACGTAAATGGGAC

Coding sequence (CDS)

ATGGATCATACAAAGCCAATTCTTTGCGTTCTTGACGCTTCCTCTTACGTGGGCTTTTGGGTCACCAAAGAATTGCTAAACAAAGGATACCCAGTTCATGCAGCCGTCCAAAACAATGGAAAGCCAGAGATTGAGAGGACTATTAGAGAGATGGGTAAAATGAAGAATGGGCTGGTGTTTTTTTCTGTGGATATCATGGACTACCAGAGCATTCTTGTTGCTTTGAAAGGATGCTCTGGTTTGTTCTGCTGTATGGACACTCCCCATGTGTTTGATGAAAAAATGGTAGATTTGGAAGTTAGAGGGACAATTAATGTTGTGGAGGCTTGTGCTCAGACAGATACTGTTAATAAGATTGTCTTCACTTCTTCCTTAACTGCTGCTGTGTGGAGAGAGAATATCCTCTCTGAGAAAGATGTGGATGAAAGATGTTGGAGTGATAAAGAATTTTGCAGGAAAATGAAGTTATGGTACCCTTTAGCAAAGACACTCTCTGAACAAGCTGCTTGGGCTTTAGCAGTGGACCGTAAGCTAAAAATGGTGTCAATTAACGCAGGGCTTGTTCTTGGACCGGCTGTTGCAGAGGAAAATTCAGGGTCAACAATCTCATATCTTAAAGGAGCTGCTCAGATGTATGAAAATGGGGTGCTGGCAGTGGTTGATGTGAAATTTTTGGTGGATGTTCATATTCGAGCAATGGAAGATTCGTCCACGGGTGGTCGATATTTCTGCTTTGATCAGATAGTGAATAGTGAGGAAGAAACTGTTAAACTTGCGAATATATTGAGGCCATTGATATCTCTTCCACAAAGGTACGAAGTTCAAGGAAGGGAAGCTTTTGCTGAGAGACTCAGAACCAGGAGATTGCACAAATTGATCGAGGGCACGGCTTAA

Protein sequence

MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA
Homology
BLAST of Bhi02G000648 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.6e-110
Identity = 191/298 (64.09%), Postives = 239/298 (80.20%), Query Frame = 0

Query: 1   MDHTK--PILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGL 60
           MDH K     CVLDAS+YVGFW+ K+LL++GY VHAA++ NG+ EIE  IREM   +  L
Sbjct: 1   MDHDKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEERL 60

Query: 61  VFFSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNK 120
           V + VD++DYQSILV+LK C+ +FCC+D+P  +DEK VDLEVRG INVVEAC +T+++ K
Sbjct: 61  VVYDVDVLDYQSILVSLKTCNVVFCCLDSPEGYDEKEVDLEVRGAINVVEACGRTESIEK 120

Query: 121 IVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKL 180
           IVF+SSLTA++WR+NI ++KDVDE+CWSD++FCR  KLW+ LAK LSE+AAWALA+DR+L
Sbjct: 121 IVFSSSLTASIWRDNIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDRRL 180

Query: 181 KMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 240
            MVSIN GLV+GP+VA+ N+  T+SYLKGAAQMYENGVLA VDVKFL DVHIRA ED S 
Sbjct: 181 NMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADVHIRAFEDVSA 240

Query: 241 GGRYFCFDQIVNSEEETVKLANILRPLISLPQRY--EVQGREAFAERLRTRRLHKLIE 295
            GRYFCF+QIVN+EEE +KL   L PLI +P RY  E+ G E + ERLR  +L KL+E
Sbjct: 241 CGRYFCFNQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKLVE 298

BLAST of Bhi02G000648 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 1.3e-104
Identity = 178/289 (61.59%), Postives = 231/289 (79.93%), Query Frame = 0

Query: 9   CVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIMDY 68
           CVLDAS+YVGFW+ K LL +GY VHAA++ NG+  +E  IR+M   +  L  + VD++DY
Sbjct: 11  CVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEVYDVDVLDY 70

Query: 69  QSILVALKGCSGLFCCMDTPHVFD-EKMVDLEVRGTINVVEACAQTDTVNKIVFTSSLTA 128
           QSIL++L  C+ +FCC+D P  +D EK VDLEVRG INVVEACA+T+++ KIVF+SSLTA
Sbjct: 71  QSILISLNNCNAVFCCLDNPEGYDEEKEVDLEVRGAINVVEACARTESIEKIVFSSSLTA 130

Query: 129 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMVSINAGL 188
           A+WR+NI ++KDVDE+CWSD +FC K KLW+ LAKT SE+AAWALA+DR + MVS+N GL
Sbjct: 131 AIWRDNIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDRMVNMVSVNPGL 190

Query: 189 VLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQ 248
           ++GP+VA+ N   T+SYLKGAAQMYENGVLA VDV+F+ DVHIRA ED+S  GRYFCF+Q
Sbjct: 191 IVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVHIRAFEDTSACGRYFCFNQ 250

Query: 249 IVNSEEETVKLANILRPLISLPQRY--EVQGREAFAERLRTRRLHKLIE 295
           IVN+EEE +KL   L PLI +P R+  E+QG E + ERLR ++L+KL+E
Sbjct: 251 IVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKLVE 299

BLAST of Bhi02G000648 vs. TAIR 10
Match: AT5G58490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-40
Identity = 90/274 (32.85%), Postives = 154/274 (56.20%), Query Frame = 0

Query: 5   KPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVD 64
           + ++CV  AS  +G W+  +LL +GY VHA V+N    +  + +  +      L  F +D
Sbjct: 6   REVVCVTGASGCIGSWLVHQLLLRGYSVHATVKNLQDEKETKHLEGLEGAATRLHLFEMD 65

Query: 65  IMDYQSILVALKGCSGLF-----CCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKI 124
           ++ Y ++  A+ GCSG+F     C +D      ++++D  V+GTINV+ A A+  +V ++
Sbjct: 66  LLQYDTVSAAINGCSGVFHLASPCIVDEVQDPQKQLLDPAVKGTINVLTA-AKEASVKRV 125

Query: 125 VFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLK 184
           V TSS++A     N  ++K  +E CW+ +++CR+  LWYPL+KTL+E+AAW  A ++ L 
Sbjct: 126 VVTSSISAITPSPNWPADKIKNEECWAAEDYCRQNGLWYPLSKTLAEKAAWEFAEEKGLD 185

Query: 185 MVSINAGLVLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDS 244
           +V +N G V+GP +    + S    +  L+G  + YEN  +  V  K +   HI   ED 
Sbjct: 186 VVVVNPGTVMGPVIPPSLNASMHMLLRLLQGCTETYENFFMGSVHFKDVALAHILVYEDP 245

Query: 245 STGGRYFCFDQIVNSEEETVKLANILRPLISLPQ 271
            + GR+ C + I +  +   K+A  L P  ++P+
Sbjct: 246 YSKGRHLCVEAISHYGDFVAKVAE-LYPNYNVPK 277

BLAST of Bhi02G000648 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 8.6e-40
Identity = 97/270 (35.93%), Postives = 145/270 (53.70%), Query Frame = 0

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREM------GKMKNGLVF 66
           ++CV    SY+G  + K LL  GY V   V     PE +  + EM          N +  
Sbjct: 54  LVCVTGGVSYLGRAIVKRLLVHGYSVRIVVD---CPEDKEKVSEMEADAETASFSNMITS 113

Query: 67  FSVDIMDYQSILVALKGCSGLF---CCMDTPHV--FDEKMVDLEVRGTINVVEACAQTDT 126
               + +  S++ A  GC+G+F     +D   V  + + M +LE + + +V+EAC +T +
Sbjct: 114 VVSRLTEIDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTAS 173

Query: 127 VNKIVFTSSLTAAVWRENILSEKD---VDERCWSDKEFCRKMKLWYPLAKTLSEQAAWAL 186
           V K VFTSSL A  W++N  +  D   ++E  WSD++ C   KLWY L K  +E+AAW +
Sbjct: 174 VRKCVFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRI 233

Query: 187 AVDRKLKMVSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHI-- 246
           A  + LK+ +I   L+ GP     NS ST++YLKGA +MY NG+LA +DV  L   H+  
Sbjct: 234 ADSKGLKLATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAKAHVCL 293

Query: 247 -RAMEDSSTGGRYFCFDQIVNSEEETVKLA 260
              + + +  GRY CFD I+ S +   KLA
Sbjct: 294 WEGLGNKTAFGRYICFDTIL-SRDGAEKLA 319

BLAST of Bhi02G000648 vs. TAIR 10
Match: AT1G51410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.6e-38
Identity = 93/273 (34.07%), Postives = 150/273 (54.95%), Query Frame = 0

Query: 8   LCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIMD 67
           +CV  AS Y+  W+ K LL +GY V A+V++   P     +  +   +  L  F  ++++
Sbjct: 9   VCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLKLFKANLLE 68

Query: 68  YQSILVALKGCSGLFCCMDTPHVFDEK-----MVDLEVRGTINVVEACAQTDTVNKIVFT 127
             S   A+ GC G+F    +P   D K     ++D  V+GTINV+ +C +T +V ++V T
Sbjct: 69  EGSFDSAIDGCEGVFHTA-SPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSSVKRVVLT 128

Query: 128 SSLTAAVWRENILS-EKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMV 187
           SS+ A  +     + E  VDE  ++D ++CR  KLWY L+KTL+E AAW  A +  L++V
Sbjct: 129 SSIAAVAFNGMPRTPETIVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAKENNLQLV 188

Query: 188 SINAGLVLGPAV---AEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST 247
           SIN  +V+GP +      ++ + +S +KG AQ + N     V+VK + + HI+A E+   
Sbjct: 189 SINPAMVIGPLLQPTLNTSAAAVLSLIKG-AQTFPNATFGWVNVKDVANAHIQAFENPDA 248

Query: 248 GGRYFCFDQIVNSEEETVKLANILRPLISLPQR 272
            GRY C  + V    E V + + L P   LP++
Sbjct: 249 DGRY-CLVERVAHYSEVVNILHDLYPDFQLPEK 278

BLAST of Bhi02G000648 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.4e-40
Identity = 96/268 (35.82%), Postives = 142/268 (52.99%), Query Frame = 0

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIM 66
           ++CV  A  ++  W+ K LL KGY V   V+N   P+    +RE+   K  L     D++
Sbjct: 8   VVCVTGAGGFIASWLVKILLEKGYTVRGTVRNPDDPK-NGHLRELEGAKERLTLCKADLL 67

Query: 67  DYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIVFTSSLT 126
           DYQS+  A+ GC G+F          E+MV+  V GT NV+ A A+ + V ++VFTSS+ 
Sbjct: 68  DYQSLREAINGCDGVFHTASPVTDDPEQMVEPAVIGTKNVINAAAEAN-VRRVVFTSSIG 127

Query: 127 AAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMVSINAG 186
           A     N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ + +V IN  
Sbjct: 128 AVYMDPNRDPETVVDETCWSDPDFCKNTKNWYCYGKMVAEQAAWEEAKEKGVDLVVINPV 187

Query: 187 LVLGPAVAEENSGST---ISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYF 246
           LV GP +    + S    + YL G+A+ Y N V A VDVK +   HI   E     GRY 
Sbjct: 188 LVQGPLLQTTVNASVLHILKYLTGSAKTYANSVQAYVDVKDVALAHILLYETPEASGRYL 247

Query: 247 CFDQIVNSEEETVKLANILRPLISLPQR 272
           C + +++   + V++ +   P   +P +
Sbjct: 248 CAESVLH-RGDVVEILSKFFPEYPIPTK 272

BLAST of Bhi02G000648 vs. ExPASy Swiss-Prot
Match: A0A0B6VQ48 (Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.6e-36
Identity = 85/274 (31.02%), Postives = 154/274 (56.20%), Query Frame = 0

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIM 66
           ++CV  AS Y+  W+ K LL +GY V A+V+N   P     +  +   K  L  F  D++
Sbjct: 5   VVCVTGASGYIASWLVKLLLQRGYTVKASVRNPNDPTKTEHLLALDGAKERLQLFKADLL 64

Query: 67  DYQSILVALKGCSGLFCCMDTPHVFD-----EKMVDLEVRGTINVVEACAQTDTVNKIVF 126
           +  S   A++GC G+F    +P   D      +++D  V+GT+NV+ +C+++ ++ ++V 
Sbjct: 65  EEGSFDSAVEGCEGVFHTA-SPFYHDVTDPKAELLDPAVKGTLNVLNSCSKSPSIKRVVL 124

Query: 127 TSSLTAAVWR-ENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 186
           TSS+ A  +  +    +  VDE  ++D + C++ KLWY L+KTL+E AAW    ++ + M
Sbjct: 125 TSSIAAVAYNGKPRTPDVVVDETWFTDPDVCKESKLWYVLSKTLAEDAAWKFVKEKGIDM 184

Query: 187 VSINAGLVLGPAV---AEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSS 246
           V+IN  +V+GP +      ++ + ++ +KG A+ Y N     ++VK + + H++A E  S
Sbjct: 185 VTINPAMVIGPLLQPTLNTSAAAILNIIKG-ARTYPNASFGWINVKDVANAHVQAFEIPS 244

Query: 247 TGGRYFCFDQIVNSEEETVKLANILRPLISLPQR 272
             GRY C  + V    E +++ + L P + LP++
Sbjct: 245 ASGRY-CLVERVAHFTEVLQIIHELYPDLQLPEK 275

BLAST of Bhi02G000648 vs. ExPASy Swiss-Prot
Match: Q9S9N9 (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.1e-35
Identity = 91/267 (34.08%), Postives = 134/267 (50.19%), Query Frame = 0

Query: 8   LCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIMD 67
           +CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   K  L+    D+ D
Sbjct: 13  VCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDPK-NTHLRELEGGKERLILCKADLQD 72

Query: 68  YQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIVFTSSLTA 127
           Y+++  A+ GC G+F          E+MV+  V G   V+ A A+   V ++V TSS+ A
Sbjct: 73  YEALKAAIDGCDGVFHTASPVTDDPEQMVEPAVNGAKFVINAAAEA-KVKRVVITSSIGA 132

Query: 128 AVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMVSINAGL 187
                N   E  VDE CWSD +FC+  K WY   K ++EQAAW  A ++ + +V +N  L
Sbjct: 133 VYMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGVDLVVLNPVL 192

Query: 188 VLGPAVAEENSGS---TISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFC 247
           VLGP +    + S    + YL G+A+ Y N   A VDV+ +   H+   E  S  GRY  
Sbjct: 193 VLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEAPSASGRYLL 252

Query: 248 FDQIVNSEEETVKLANILRPLISLPQR 272
            +       E V++   L P   LP +
Sbjct: 253 AES-ARHRGEVVEILAKLFPEYPLPTK 276

BLAST of Bhi02G000648 vs. ExPASy Swiss-Prot
Match: Q9SAH9 (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.8e-35
Identity = 89/268 (33.21%), Postives = 135/268 (50.37%), Query Frame = 0

Query: 7   ILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVFFSVDIM 66
           ++CV  A  Y+  W+ K LL +GY V   V+N   P+    +RE+   K  L   S D++
Sbjct: 7   LVCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDPK-NNHLRELQGAKERLTLHSADLL 66

Query: 67  DYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIVFTSSLT 126
           DY+++   + GC G+F          E M++  V G   V++A A+   V ++VFTSS+ 
Sbjct: 67  DYEALCATIDGCDGVFHTASPMTDDPETMLEPAVNGAKFVIDAAAKA-KVKRVVFTSSIG 126

Query: 127 AAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKMVSINAG 186
           A     N  ++  VDE CWSD +FC+  K WY   K L+EQ+AW  A  + + +V +N  
Sbjct: 127 AVYMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDLVVLNPV 186

Query: 187 LVLGPAVAEENSGSTI---SYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYF 246
           LVLGP +    + S +    YL G+A+ Y N     VDV+ +   H+   E  S  GRY 
Sbjct: 187 LVLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPSASGRYI 246

Query: 247 CFDQIVNSEEETVKLANILRPLISLPQR 272
             +  ++  E    LA    P   LP +
Sbjct: 247 LAETALHRGEVVEILAKFF-PEYPLPTK 271

BLAST of Bhi02G000648 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.4e-32
Identity = 77/285 (27.02%), Postives = 146/285 (51.23%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MD  K  +CV  AS ++  W+ K LL +GY V   V++ G  +    + ++   K  L  
Sbjct: 1   MDQAKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLRL 60

Query: 61  FSVDIMDYQSILVALKGCSGLF----CCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTV 120
              D+M+  S   A+ GC G+F      +      +E+++   + GT+NV+ +C +  ++
Sbjct: 61  VKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSL 120

Query: 121 NKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDR 180
            ++V TSS +    R++   +  +DE  W+  E C++ ++WY L+KTL+EQAAW  + + 
Sbjct: 121 KRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFSEEN 180

Query: 181 KLKMVSINAGLVLGPAVAEE---NSGSTISYLKGAAQMYE-NGVLAVVDVKFLVDVHIRA 240
            + +V++    ++GP++  +    +   +  LKG  + ++ +G +  V +  +   HI  
Sbjct: 181 GIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTHIVV 240

Query: 241 MEDSSTGGRYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGR 278
            E  +  GRY C   ++ S EE V   +   P + +P+R+E   R
Sbjct: 241 FEHEAAQGRYICSSNVI-SLEELVSFLSARYPSLPIPKRFEKLNR 284

BLAST of Bhi02G000648 vs. NCBI nr
Match: XP_038879610.1 (cinnamoyl-CoA reductase-like SNL6 isoform X1 [Benincasa hispida])

HSP 1 Score: 598.2 bits (1541), Expect = 3.9e-167
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF
Sbjct: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 297

BLAST of Bhi02G000648 vs. NCBI nr
Match: XP_038879611.1 (cinnamoyl-CoA reductase-like SNL6 isoform X2 [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.0e-163
Identity = 294/297 (98.99%), Postives = 294/297 (98.99%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNN   EIERTIREMGKMKNGLVF
Sbjct: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNN---EIERTIREMGKMKNGLVF 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 294

BLAST of Bhi02G000648 vs. NCBI nr
Match: XP_011656009.1 (cinnamoyl-CoA reductase-like SNL6 [Cucumis sativus] >KGN65259.1 hypothetical protein Csa_019971 [Cucumis sativus] >QHJ91598.1 cinnamoyl-CoA reductase 1 [Cucumis sativus])

HSP 1 Score: 567.0 bits (1460), Expect = 9.6e-158
Identity = 279/297 (93.94%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQN GKPEIE+TIREMG+++NGL  
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQRYEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of Bhi02G000648 vs. NCBI nr
Match: XP_008443817.1 (PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 561.2 bits (1445), Expect = 5.2e-156
Identity = 278/297 (93.60%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MD +KPILCVLDASSYVGFWVTK LL+KGYPVHAAVQN GKPEIE+TIREM K++NGL+ 
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQG+EAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 297

BLAST of Bhi02G000648 vs. NCBI nr
Match: KAG6589749.1 (Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 270/297 (90.91%), Postives = 288/297 (96.97%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           M+H KP LCVLDASSYVGFW+TKELLN+GYPVHAAV+  G PE+E+TIREMG+++NGLV 
Sbjct: 1   MEHRKPTLCVLDASSYVGFWITKELLNRGYPVHAAVRKTGNPEMEKTIREMGEVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           F VDIMDY SILVALKGCSGLFCCMDTPHV+DEKMV+LEVRGTINVVEACAQTDTV+K V
Sbjct: 61  FPVDIMDYHSILVALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACAQTDTVHKTV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           +TSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 YTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTRRLHKL+EGTA
Sbjct: 241 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 297

BLAST of Bhi02G000648 vs. ExPASy TrEMBL
Match: A0A0A0LW90 (Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.6e-158
Identity = 279/297 (93.94%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MDH+KP LCVLDASSYVGFWVTK LLNKGYPVHAAVQN GKPEIE+TIREMG+++NGL  
Sbjct: 1   MDHSKPTLCVLDASSYVGFWVTKGLLNKGYPVHAAVQNTGKPEIEKTIREMGEVENGLAV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLIS+PQRYEVQGREAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of Bhi02G000648 vs. ExPASy TrEMBL
Match: A0A1S3B8Z2 (cinnamoyl-CoA reductase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487321 PE=4 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.5e-156
Identity = 278/297 (93.60%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MD +KPILCVLDASSYVGFWVTK LL+KGYPVHAAVQN GKPEIE+TIREM K++NGL+ 
Sbjct: 1   MDPSKPILCVLDASSYVGFWVTKGLLSKGYPVHAAVQNTGKPEIEKTIREMCKVENGLMV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           FSVDIMDYQSILVALKGCSGLFCCMDT HV+DEKMVDLEVRGTINVVEACAQTD+V KIV
Sbjct: 61  FSVDIMDYQSILVALKGCSGLFCCMDTHHVYDEKMVDLEVRGTINVVEACAQTDSVEKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQG+EAFAERLRTR+LHKLIEGTA
Sbjct: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGKEAFAERLRTRKLHKLIEGTA 297

BLAST of Bhi02G000648 vs. ExPASy TrEMBL
Match: A0A6J1E516 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111430012 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.0e-154
Identity = 268/297 (90.24%), Postives = 286/297 (96.30%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           M+H KP LCVLDASSYVGFW+TKELLN+GYPVHAAV+  G PE+E+TIRE G+++NGLV 
Sbjct: 12  MEHRKPTLCVLDASSYVGFWITKELLNRGYPVHAAVRKTGNPEMEKTIRETGEVENGLVV 71

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           F VDIMDY SIL ALKGCSGLFCCMDTPHV+DEKMV+LEVRGTINVVEACAQTDTV+K V
Sbjct: 72  FPVDIMDYHSILAALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACAQTDTVHKTV 131

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           +TSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 132 YTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 191

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 192 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 251

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTRRLHKL+EGTA
Sbjct: 252 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 308

BLAST of Bhi02G000648 vs. ExPASy TrEMBL
Match: A0A6J1JM00 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111485788 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 5.9e-153
Identity = 268/297 (90.24%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           M+H K  LCVLDASSYVGFW+TK LLN+GYPVHAAV+  G PEIE+ IREMGK++NGLV 
Sbjct: 1   MEHRKLTLCVLDASSYVGFWITKGLLNRGYPVHAAVRKTGNPEIEKAIREMGKVENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           F VDIMDY SIL+ALKGCSGLFCCMDTPHV+DEKMV+LEVRGTINVVEACA+TDTV+K V
Sbjct: 61  FPVDIMDYHSILIALKGCSGLFCCMDTPHVYDEKMVELEVRGTINVVEACARTDTVHKTV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALA+DR+LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAMDRRLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAE+NSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG
Sbjct: 181 VSINAGLVLGPAVAEQNSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLANILRPLISLPQRYEVQGREAFAERLRTRRLHKL+EGTA
Sbjct: 241 RYFCFDQIVNSEEETLKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLVEGTA 297

BLAST of Bhi02G000648 vs. ExPASy TrEMBL
Match: A0A6J1C504 (cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111007448 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.1e-151
Identity = 270/297 (90.91%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 1   MDHTKPILCVLDASSYVGFWVTKELLNKGYPVHAAVQNNGKPEIERTIREMGKMKNGLVF 60
           MD  KP LCVLDAS+YVGFWVTKELLN+GYPVHAAVQN+GK EIE+TIREMGK++NGLV 
Sbjct: 1   MDPQKPTLCVLDASTYVGFWVTKELLNRGYPVHAAVQNSGKSEIEKTIREMGKLENGLVV 60

Query: 61  FSVDIMDYQSILVALKGCSGLFCCMDTPHVFDEKMVDLEVRGTINVVEACAQTDTVNKIV 120
           F VDIMDYQSILVALKGCSGLFCCMDTP V+DEKMVDLEVRGTINVVEACAQT+T+NKIV
Sbjct: 61  FPVDIMDYQSILVALKGCSGLFCCMDTPQVYDEKMVDLEVRGTINVVEACAQTETLNKIV 120

Query: 121 FTSSLTAAVWRENILSEKDVDERCWSDKEFCRKMKLWYPLAKTLSEQAAWALAVDRKLKM 180
           FTSSLTAAVWRENILSEKDVDERCWSD+EFCRKMKLWYPLAKTLSEQAAWALA+DR LKM
Sbjct: 121 FTSSLTAAVWRENILSEKDVDERCWSDQEFCRKMKLWYPLAKTLSEQAAWALAMDRMLKM 180

Query: 181 VSINAGLVLGPAVAEENSGSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 240
           VSINAGLVLGPAVAEENS STISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSST G
Sbjct: 181 VSINAGLVLGPAVAEENSVSTISYLKGAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTCG 240

Query: 241 RYFCFDQIVNSEEETVKLANILRPLISLPQRYEVQGREAFAERLRTRRLHKLIEGTA 298
           RYFCFDQIVNSEEET+KLAN LRPLISLP RYE QGREAFAERLRTRRLHKL+E TA
Sbjct: 241 RYFCFDQIVNSEEETLKLANTLRPLISLPSRYECQGREAFAERLRTRRLHKLVEATA 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G30470.11.6e-11064.09NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G23910.11.3e-10461.59NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58490.11.3e-4032.85NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G14700.18.6e-4035.93NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G51410.11.6e-3834.07NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
A0A059TC028.4e-4035.82Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
A0A0B6VQ489.6e-3631.02Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1[more]
Q9S9N92.1e-3534.08Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1[more]
Q9SAH92.8e-3533.21Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1[more]
Q500U88.4e-3227.02Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Match NameE-valueIdentityDescription
XP_038879610.13.9e-167100.00cinnamoyl-CoA reductase-like SNL6 isoform X1 [Benincasa hispida][more]
XP_038879611.12.0e-16398.99cinnamoyl-CoA reductase-like SNL6 isoform X2 [Benincasa hispida][more]
XP_011656009.19.6e-15893.94cinnamoyl-CoA reductase-like SNL6 [Cucumis sativus] >KGN65259.1 hypothetical pro... [more]
XP_008443817.15.2e-15693.60PREDICTED: cinnamoyl-CoA reductase 1 isoform X1 [Cucumis melo][more]
KAG6589749.11.3e-15490.91Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A0A0LW904.6e-15893.94Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1[more]
A0A1S3B8Z22.5e-15693.60cinnamoyl-CoA reductase 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487321 PE=... [more]
A0A6J1E5169.0e-15490.24cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111430012 ... [more]
A0A6J1JM005.9e-15390.24cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111485788 PE... [more]
A0A6J1C5041.1e-15190.91cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111007448... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 13..169
e-value: 9.7E-9
score: 34.6
NoneNo IPR availableGENE3D3.40.50.720coord: 2..294
e-value: 3.3E-65
score: 222.4
NoneNo IPR availablePANTHERPTHR10366:SF390CINNAMOYL-COA REDUCTASE-LIKE PROTEINcoord: 3..295
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 3..295
NoneNo IPR availableCDDcd08958FR_SDR_ecoord: 9..292
e-value: 2.57384E-111
score: 322.217
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000648Bhi02M000648mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor