Bhi02G000450 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000450
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCytochrome P450, putative
Locationchr2: 11273521 .. 11274105 (-)
RNA-Seq ExpressionBhi02G000450
SyntenyBhi02G000450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGCAGCAGGTACAGACACAACTTACACAGTATTGGAATGGGCGATGTCAAAATTGTTGAAACATCCAAAAACCATGAAGAAACTGAAGAATGAAATCAGAAAAATAAAAGGTGATCACAAAGGATGTTATGTAAATGAGAATGATTTAGACAAAATGGTTTATTTAAAGGCAATAATCAAAGAGACGTTGCGACTGCACCCACCCATTCCACTTCTCATTCCTAAAGAATCAATCAAACCCATAAATTTAAGAGGGTATGACATCAAACCTAGAACTCGAGTTATAAGCAATGTCGCCCCAAAACTATGGGAAGAAGCAGAGGAGTTTAGGCTAGAAAGGTTTATGAACAATTCAATAGATTTCAAGGGTCAGGATTTCGTGTTGATCCCATTTGGTGTAGGGAGAAGAGGATGTCCTGGGATTGTGTTTGCAACTGTAATTAATGAGATAACTTTAGCAAATTTGGTGCATAAGTTTGATTGGATGGTTTCAAATGGTGAGGTTTTGGACATGACAGGAGCTTTTGGGATGACCATTCATAGGAAGTTTCCACTTGTGGCTACAACAACTCCTTGTTAG

mRNA sequence

ATGTTTGCAGCAGGTACAGACACAACTTACACAGTATTGGAATGGGCGATGTCAAAATTGTTGAAACATCCAAAAACCATGAAGAAACTGAAGAATGAAATCAGAAAAATAAAAGGTGATCACAAAGGATGTTATGTAAATGAGAATGATTTAGACAAAATGGTTTATTTAAAGGCAATAATCAAAGAGACGTTGCGACTGCACCCACCCATTCCACTTCTCATTCCTAAAGAATCAATCAAACCCATAAATTTAAGAGGGTATGACATCAAACCTAGAACTCGAGTTATAAGCAATGTCGCCCCAAAACTATGGGAAGAAGCAGAGGAGTTTAGGCTAGAAAGGTTTATGAACAATTCAATAGATTTCAAGGGTCAGGATTTCGTGTTGATCCCATTTGGTGTAGGGAGAAGAGGATGTCCTGGGATTGTGTTTGCAACTGTAATTAATGAGATAACTTTAGCAAATTTGGTGCATAAGTTTGATTGGATGGTTTCAAATGGTGAGGTTTTGGACATGACAGGAGCTTTTGGGATGACCATTCATAGGAAGTTTCCACTTGTGGCTACAACAACTCCTTGTTAG

Coding sequence (CDS)

ATGTTTGCAGCAGGTACAGACACAACTTACACAGTATTGGAATGGGCGATGTCAAAATTGTTGAAACATCCAAAAACCATGAAGAAACTGAAGAATGAAATCAGAAAAATAAAAGGTGATCACAAAGGATGTTATGTAAATGAGAATGATTTAGACAAAATGGTTTATTTAAAGGCAATAATCAAAGAGACGTTGCGACTGCACCCACCCATTCCACTTCTCATTCCTAAAGAATCAATCAAACCCATAAATTTAAGAGGGTATGACATCAAACCTAGAACTCGAGTTATAAGCAATGTCGCCCCAAAACTATGGGAAGAAGCAGAGGAGTTTAGGCTAGAAAGGTTTATGAACAATTCAATAGATTTCAAGGGTCAGGATTTCGTGTTGATCCCATTTGGTGTAGGGAGAAGAGGATGTCCTGGGATTGTGTTTGCAACTGTAATTAATGAGATAACTTTAGCAAATTTGGTGCATAAGTTTGATTGGATGGTTTCAAATGGTGAGGTTTTGGACATGACAGGAGCTTTTGGGATGACCATTCATAGGAAGTTTCCACTTGTGGCTACAACAACTCCTTGTTAG

Protein sequence

MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAIIKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNVAPKLWEEAEEFRLERFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTGAFGMTIHRKFPLVATTTPC
Homology
BLAST of Bhi02G000450 vs. TAIR 10
Match: AT5G24960.1 (cytochrome P450, family 71, subfamily A, polypeptide 14 )

HSP 1 Score: 191.4 bits (485), Expect = 6.6e-49
Identity = 97/202 (48.02%), Postives = 139/202 (68.81%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MF AGT+TTY +LEW M++L++HP+ MKKL++EIR  K      Y++E D++ M YLKA+
Sbjct: 296 MFLAGTETTYALLEWIMTELIRHPECMKKLQDEIR-AKATKLILYISEEDVEDMKYLKAV 355

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWE-EAEEFRLE 120
           +KE LRLHPP+PLL+P+E  + I L+GYDI   T+VI N          W  +AEEFR E
Sbjct: 356 VKEVLRLHPPLPLLVPRELSEDIKLKGYDIAAGTQVIINAWAIQRDTMTWGIDAEEFRPE 415

Query: 121 RFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVS---NGEVL 180
           R +++ +DF+G +F  IPFG GRR CPGI FA  + E+TLANLV++F+W +    +G+  
Sbjct: 416 RHLDSLVDFRGTNFEFIPFGSGRRICPGIGFAMALVEVTLANLVNRFNWRMEVQHSGDEY 475

Query: 181 DMTGAFGMTIHRKFPLVATTTP 194
           D+  + G+ + RKFPL+   +P
Sbjct: 476 DLAESTGLDVCRKFPLIVFPSP 496

BLAST of Bhi02G000450 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 189.9 bits (481), Expect = 1.9e-48
Identity = 95/203 (46.80%), Postives = 138/203 (67.98%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKI-KGDHKGCYVNENDLDKMVYLKA 60
           +F  G+DT++T+LEWAM++LL+HPK++ +L+ E+R I KG  +   V+E+D+  M YLKA
Sbjct: 288 VFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTICKGKSR---VSEDDIQGMKYLKA 347

Query: 61  IIKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLW-EEAEEFRL 120
           +IKE LRLHPP P++ P ES + + LR Y I   T+V+ N          W  +AEEF+ 
Sbjct: 348 VIKEALRLHPPFPMMAPHESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKP 407

Query: 121 ERFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMV---SNGEV 180
           ER ++ S+DF+GQ+F L+PFG GRR CP + FA V+NE+ LANLVH FDW +   S  + 
Sbjct: 408 ERHLDTSVDFRGQNFELLPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDK 467

Query: 181 LDMTGAFGMTIHRKFPLVATTTP 194
            D+  + G ++HR+FPL A  +P
Sbjct: 468 TDVAESSGFSVHREFPLYAVASP 487

BLAST of Bhi02G000450 vs. TAIR 10
Match: AT3G48290.1 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 188.3 bits (477), Expect = 5.6e-48
Identity = 98/201 (48.76%), Postives = 131/201 (65.17%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           +F    DTTYT+LEWAM++LL H + + +L+ E+R +  D  G  V+E+DL  M YLKA+
Sbjct: 290 VFVGDMDTTYTLLEWAMTELLCHHECLDRLQEEVRMVCKDKSG--VSEDDLQDMKYLKAV 349

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV------APKLWEEAEEFRLE 120
           IKETLRLHPP+PL++P ES   + LR Y I   T V+ N       A     +AEEFR E
Sbjct: 350 IKETLRLHPPLPLMVPHESTHDVKLRDYHIPAGTHVMINAWAIGREAATWGPDAEEFRPE 409

Query: 121 RFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMV---SNGEVL 180
           R +N+ +D++GQD  L+PFG GRR CP I FA V++E+ LANLVH+FDW +   S     
Sbjct: 410 RHLNSYVDYRGQDTELVPFGAGRRICPAISFAVVLDEVVLANLVHQFDWTLPEESTEYQT 469

Query: 181 DMTGAFGMTIHRKFPLVATTT 193
           D+  + GM +HR FPL A TT
Sbjct: 470 DVAESTGMAVHRMFPLFAMTT 488

BLAST of Bhi02G000450 vs. TAIR 10
Match: AT3G48290.2 (cytochrome P450, family 71, subfamily A, polypeptide 24 )

HSP 1 Score: 188.3 bits (477), Expect = 5.6e-48
Identity = 98/201 (48.76%), Postives = 131/201 (65.17%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           +F    DTTYT+LEWAM++LL H + + +L+ E+R +  D  G  V+E+DL  M YLKA+
Sbjct: 314 VFVGDMDTTYTLLEWAMTELLCHHECLDRLQEEVRMVCKDKSG--VSEDDLQDMKYLKAV 373

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV------APKLWEEAEEFRLE 120
           IKETLRLHPP+PL++P ES   + LR Y I   T V+ N       A     +AEEFR E
Sbjct: 374 IKETLRLHPPLPLMVPHESTHDVKLRDYHIPAGTHVMINAWAIGREAATWGPDAEEFRPE 433

Query: 121 RFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMV---SNGEVL 180
           R +N+ +D++GQD  L+PFG GRR CP I FA V++E+ LANLVH+FDW +   S     
Sbjct: 434 RHLNSYVDYRGQDTELVPFGAGRRICPAISFAVVLDEVVLANLVHQFDWTLPEESTEYQT 493

Query: 181 DMTGAFGMTIHRKFPLVATTT 193
           D+  + GM +HR FPL A TT
Sbjct: 494 DVAESTGMAVHRMFPLFAMTT 512

BLAST of Bhi02G000450 vs. TAIR 10
Match: AT3G48270.1 (cytochrome P450, family 71, subfamily A, polypeptide 26 )

HSP 1 Score: 185.3 bits (469), Expect = 4.8e-47
Identity = 95/198 (47.98%), Postives = 131/198 (66.16%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           +F  GTDT+ T++EWAM++LL+HPK +K+L+ E+R I  D     V+E ++  M YLKA+
Sbjct: 288 VFVGGTDTSSTLMEWAMTELLRHPKCLKRLQEEVRTICKDKSS--VSEEEIQNMSYLKAV 347

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV------APKLWEEAEEFRLE 120
           IKE LRLHPP+PL++P ES + + L  + I   T+V+ N       A     + EEFR E
Sbjct: 348 IKEALRLHPPLPLMVPHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPE 407

Query: 121 RFMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMT 180
           R +++S+D++GQ F LIPFG GRR CP I FA V+NE+ LANLVH+FDW +S     D T
Sbjct: 408 RHLDSSVDYRGQAFELIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQT 467

Query: 181 ---GAFGMTIHRKFPLVA 190
               + G+ IHR FPL A
Sbjct: 468 EVAESTGIAIHRMFPLYA 483

BLAST of Bhi02G000450 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 4.0e-59
Identity = 113/201 (56.22%), Postives = 146/201 (72.64%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDTTY+ LEWAM++LL+HP+ M KL+NE+R I G+     + E+DL +M YLKA+
Sbjct: 317 MFAAGTDTTYSALEWAMTELLRHPRVMNKLQNEVRGIVGNRTD-VITEDDLVEMHYLKAV 376

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
            KETLRLHPPIPLL+P+ S + + + GY+IK  T+V  +       PKL+++ EEF  ER
Sbjct: 377 TKETLRLHPPIPLLVPRMSTRDVEVNGYNIKANTQVFISAWQIGRDPKLYDKPEEFEPER 436

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMV---SNGEVLD 180
           F+NN ID+KG DF LIPFG GRR CPGI FA  +NEI LAN+VHKFDW +   ++GE LD
Sbjct: 437 FLNNGIDYKGNDFELIPFGAGRRVCPGIQFAMAVNEIALANIVHKFDWALPDEASGEDLD 496

Query: 181 MTGAFGMTIHRKFPLVATTTP 194
           MT   G+T H+K+PL A   P
Sbjct: 497 MTETTGLTAHKKYPLKAVAFP 516

BLAST of Bhi02G000450 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 1.5e-53
Identity = 108/195 (55.38%), Postives = 135/195 (69.23%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MF+ GTDTT   LEWAM++L+KHP  M+K + E+R++ G  K   V E DL ++ YLK I
Sbjct: 301 MFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVG--KKAKVEEEDLHQLHYLKLI 360

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           IKETLRLHP  PLL+P+ES + + +RGY I  +TRV  N       PK WE AEEF  ER
Sbjct: 361 IKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPER 420

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNG---EVLD 180
           F+NNS+DFKGQDF LIPFG GRRGCPGI F     EI+LANL++ F+W +      E LD
Sbjct: 421 FVNNSVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLD 480

Query: 181 MTGAFGMTIHRKFPL 188
           M+ A G+T+H KFPL
Sbjct: 481 MSEAVGITVHMKFPL 493

BLAST of Bhi02G000450 vs. ExPASy Swiss-Prot
Match: O04164 (Cytochrome P450 71A6 (Fragment) OS=Nepeta racemosa OX=54731 GN=CYP71A6 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.5e-53
Identity = 107/201 (53.23%), Postives = 140/201 (69.65%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MF AGTDTT T LEWA+S+L+K+P+ MK L+ E+R + G  KG  + E+DL+KM YLKA+
Sbjct: 308 MFLAGTDTTVTALEWALSELIKNPRAMKILQKEVRGVAGS-KG-EIEESDLEKMPYLKAV 367

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KE+LRLH P+PLL+P+ES +   + GYD+   TRV+ N         +WEE+E F  ER
Sbjct: 368 MKESLRLHAPVPLLVPRESTRDTKVLGYDVASGTRVLINCWAIGRDSSVWEESETFLPER 427

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNG---EVLD 180
           F+  SID++G  F LIPFG GRRGCPG  FA  I+E+ LA LVHKFD+ + NG   E LD
Sbjct: 428 FLETSIDYRGMHFELIPFGSGRRGCPGATFAAAIDELALATLVHKFDFKLPNGVRVEDLD 487

Query: 181 MTGAFGMTIHRKFPLVATTTP 194
           M+   G TIH+KFPL+   TP
Sbjct: 488 MSEGSGFTIHKKFPLLVVPTP 506

BLAST of Bhi02G000450 vs. ExPASy Swiss-Prot
Match: Q947B7 ((+)-menthofuran synthase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 7.7e-50
Identity = 99/201 (49.25%), Postives = 136/201 (67.66%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           M +AGTDTT+  LEW M++L+K+P+T+K L+NE+R++  +  G  + E+D+DKM YLKA+
Sbjct: 292 MVSAGTDTTFAALEWTMAELIKNPRTLKTLQNEVREVSRNKGG--ITEDDVDKMPYLKAV 351

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISN-----VAPKLWEEAEEFRLER 120
            KE LRLHPP  +L+P+E  +  N+ GYDI   T V+ N       P LWE  EEFR ER
Sbjct: 352 SKEILRLHPPFAILLPRELTQDANMLGYDIPRGTVVLVNNWAISRDPSLWENPEEFRPER 411

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNG---EVLD 180
           F+  SID+KG  F ++PFG GRRGCPG  FA  + E+ L+ LV++FD+ + NG   E LD
Sbjct: 412 FLETSIDYKGLHFEMLPFGSGRRGCPGSTFAMALYELALSKLVNEFDFRLGNGDRAEDLD 471

Query: 181 MTGAFGMTIHRKFPLVATTTP 194
           MT A G  +H+K PL+   TP
Sbjct: 472 MTEAPGFVVHKKSPLLVLATP 490

BLAST of Bhi02G000450 vs. ExPASy Swiss-Prot
Match: Q42716 (Cytochrome P450 71A8 OS=Mentha piperita OX=34256 GN=CYP71A8 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.9e-49
Identity = 99/198 (50.00%), Postives = 135/198 (68.18%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           +FAAGTDTT  VLEWAM++LL+HP+ MKKL++E+R++  D     + ++D++KM YLKA+
Sbjct: 305 VFAAGTDTTAVVLEWAMTELLRHPEIMKKLQSEVRQVVKDKHN--ITDDDIEKMHYLKAV 364

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KET+R H PIPLL+P+ +   + + GYD+   T V+ N       P  W+E E+FR ER
Sbjct: 365 MKETMRFHTPIPLLVPRVARNDVEVMGYDVPVGTMVMINAWAIGRDPTSWDEPEKFRPER 424

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNG-EVLDMT 180
           F+N+S+DFKG DF LIPFG GRRGCPG  F     E TLANL+ KFDW + +    LDM+
Sbjct: 425 FLNSSVDFKGLDFELIPFGAGRRGCPGTTFPMATLEFTLANLMQKFDWELPHECRELDMS 484

Query: 181 GAFGMTIHRKFPLVATTT 193
              G+ I R  PL+A  T
Sbjct: 485 ERPGVAIRRVIPLLAIGT 500

BLAST of Bhi02G000450 vs. NCBI nr
Match: XP_038880328.1 (cytochrome P450 736A117-like [Benincasa hispida])

HSP 1 Score: 399.8 bits (1026), Expect = 1.3e-107
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI
Sbjct: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNVAPKLWEEAEEFRLERFMNNS 120
           IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNVAPKLWEEAEEFRLERFMNNS
Sbjct: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNVAPKLWEEAEEFRLERFMNNS 120

Query: 121 IDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTGAFGMT 180
           IDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTGAFGMT
Sbjct: 121 IDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTGAFGMT 180

Query: 181 IHRKFPLVATTTPC 195
           IHRKFPLVATTTPC
Sbjct: 181 IHRKFPLVATTTPC 194

BLAST of Bhi02G000450 vs. NCBI nr
Match: XP_038880242.1 (cytochrome P450 736A117-like [Benincasa hispida])

HSP 1 Score: 345.9 bits (886), Expect = 2.3e-91
Identity = 168/199 (84.42%), Postives = 177/199 (88.94%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDTTYTVLEWAMS+LLKHP+TMKKLKNEIR+IKGDHK CYVNENDL+KMVYLKA+
Sbjct: 307 MFAAGTDTTYTVLEWAMSELLKHPETMKKLKNEIREIKGDHKRCYVNENDLEKMVYLKAV 366

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           IKETLRLHPPIPLL+P+ESI+PI LRGYDI P TRVI N       PKLW EAEEFR ER
Sbjct: 367 IKETLRLHPPIPLLVPRESIRPIKLRGYDINPGTRVIINAWAIGRDPKLWGEAEEFRPER 426

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKGQDF LIPFG GRRGCPGIVFAT INEI LANLVHKFDWMV NGE LDMTG
Sbjct: 427 FMNNSIDFKGQDFELIPFGAGRRGCPGIVFATAINEIALANLVHKFDWMVPNGEGLDMTG 486

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHR FPLVAT TPC
Sbjct: 487 AFGLTIHRNFPLVATATPC 505

BLAST of Bhi02G000450 vs. NCBI nr
Match: XP_038880557.1 (cytochrome P450 736A117-like [Benincasa hispida])

HSP 1 Score: 345.9 bits (886), Expect = 2.3e-91
Identity = 168/199 (84.42%), Postives = 177/199 (88.94%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDTTYTVLEWAMS+LLKHP+TMKKLKNEIR+IKGDHK CYVNENDL+KMVYLKA+
Sbjct: 306 MFAAGTDTTYTVLEWAMSELLKHPETMKKLKNEIREIKGDHKRCYVNENDLEKMVYLKAV 365

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           IKETLRLHPPIPLL+P+ESI+PI LRGYDI P TRVI N       PKLW EAEEFR ER
Sbjct: 366 IKETLRLHPPIPLLVPRESIRPIKLRGYDINPGTRVIINAWAIGRDPKLWGEAEEFRPER 425

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKGQDF LIPFG GRRGCPGIVFAT INEI LANLVHKFDWMV NGE LDMTG
Sbjct: 426 FMNNSIDFKGQDFELIPFGAGRRGCPGIVFATAINEIALANLVHKFDWMVPNGEGLDMTG 485

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHR FPLVAT TPC
Sbjct: 486 AFGLTIHRNFPLVATATPC 504

BLAST of Bhi02G000450 vs. NCBI nr
Match: XP_004138089.1 (cytochrome P450 71A22 [Cucumis sativus] >KGN63544.2 hypothetical protein Csa_013716 [Cucumis sativus])

HSP 1 Score: 320.9 bits (821), Expect = 7.8e-84
Identity = 152/199 (76.38%), Postives = 176/199 (88.44%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDTTYTVLEWAMS+LLKHP+ MKKLKNEIR+IKG+HKG YVNE+DL+KMVYLKA+
Sbjct: 311 MFAAGTDTTYTVLEWAMSELLKHPEVMKKLKNEIREIKGEHKGSYVNEDDLNKMVYLKAV 370

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
            KETLRLH PIPLL+P+ESIKP+ L GYDIKP TRV+ N       PK+WEEAE+F+ ER
Sbjct: 371 FKETLRLHTPIPLLVPRESIKPVKLGGYDIKPGTRVMINAWTIGRDPKVWEEAEKFQPER 430

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMN+SIDFKGQDF LIPFG GRRGCPGI+FA +++EITLANLVHKF+W++ NGE LDMTG
Sbjct: 431 FMNSSIDFKGQDFELIPFGAGRRGCPGIMFAAMVSEITLANLVHKFEWILPNGEDLDMTG 490

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG++IHRKFPLVAT  PC
Sbjct: 491 AFGLSIHRKFPLVATAIPC 509

BLAST of Bhi02G000450 vs. NCBI nr
Match: XP_023515572.1 (cytochrome P450 71A24-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.4 bits (812), Expect = 8.6e-83
Identity = 152/199 (76.38%), Postives = 172/199 (86.43%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGT+TT TVLEW MS+LLKHP+ MKKLK+EIR+I GDHK  YVNE+DLDKM YLKA+
Sbjct: 306 MFAAGTETTSTVLEWTMSELLKHPEVMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAV 365

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KETLRLHPPIPLL+P+ES + I LRGYDIKP+TRV+ N       P++W+EAEEFR ER
Sbjct: 366 LKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWDEAEEFRPER 425

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKGQDF LIPFG GRRGCPGI FATVINE+ LANLVHKF+WM+ NGE LDMTG
Sbjct: 426 FMNNSIDFKGQDFELIPFGAGRRGCPGIAFATVINEVALANLVHKFEWMLPNGEDLDMTG 485

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHRK PLVAT TPC
Sbjct: 486 AFGLTIHRKHPLVATATPC 504

BLAST of Bhi02G000450 vs. ExPASy TrEMBL
Match: A0A5A7UWH3 (Cytochrome P450 71A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001060 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.6e-82
Identity = 149/199 (74.87%), Postives = 172/199 (86.43%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDT YTVLEWAMS+LLKHP+ MKKLKNEI +IKG+HKG Y+NE+D+DKMVYLKAI
Sbjct: 311 MFAAGTDTAYTVLEWAMSELLKHPEVMKKLKNEIGEIKGEHKGSYINEDDIDKMVYLKAI 370

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           IKETLRLH PIPLL+P+ESIKP+ LRGYDIKP TRV+ N       PK+WEEAE+F+ ER
Sbjct: 371 IKETLRLHTPIPLLVPRESIKPVKLRGYDIKPGTRVMINAWTIGRDPKVWEEAEKFQPER 430

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           F+N+SIDFKGQDF LIPFG GRRGCPGI FAT++ EI LANLVHKF+W++ NGE LDM+G
Sbjct: 431 FLNSSIDFKGQDFELIPFGAGRRGCPGIAFATMVMEIALANLVHKFEWILPNGEDLDMSG 490

Query: 181 AFGMTIHRKFPLVATTTPC 195
           A G+TIHRK PLVAT  PC
Sbjct: 491 ASGLTIHRKIPLVATAVPC 509

BLAST of Bhi02G000450 vs. ExPASy TrEMBL
Match: A0A6J1E6T0 (cytochrome P450 71A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.6e-82
Identity = 151/199 (75.88%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MF+AGTDT  TVLEWAMS+LLKHP+ MKKLK+EIR+I GDHK  YVNE+DLDKM YLKA+
Sbjct: 306 MFSAGTDTISTVLEWAMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAV 365

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KETLRLHPPIPLL+P+ES + I L+GYDIKP+TRV+ N       P++W+EAEEFR ER
Sbjct: 366 LKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAEEFRPER 425

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKG DF LIPFG GRRGCPGI FATVINEI LANLVHKF+WM+ NGE LDMTG
Sbjct: 426 FMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIALANLVHKFEWMLPNGEDLDMTG 485

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHRK PLVAT TPC
Sbjct: 486 AFGLTIHRKHPLVATATPC 504

BLAST of Bhi02G000450 vs. ExPASy TrEMBL
Match: A0A6J1E4T4 (cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.0e-82
Identity = 150/199 (75.38%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGT+TT TV+EW MS+LLKHP+ MKKLK+EIR+I GDHK  YVNE+DLDKM YLKA+
Sbjct: 306 MFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAV 365

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KETLRLHPPIPLL+P+ES + I L+GYDIKP+TRV+ N       P++W+EAEEFR ER
Sbjct: 366 LKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAEEFRPER 425

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKG DF LIPFG GRRGCPGI FATVINEI LANLVHKF+WM+ NGE LDMTG
Sbjct: 426 FMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIALANLVHKFEWMLPNGEDLDMTG 485

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHRK PLVAT TPC
Sbjct: 486 AFGLTIHRKHPLVATATPC 504

BLAST of Bhi02G000450 vs. ExPASy TrEMBL
Match: A0A6J1E1I6 (cytochrome P450 71A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429935 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.0e-82
Identity = 150/199 (75.38%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGT+TT TV+EW MS+LLKHP+ MKKLK+EIR+I GDHK  YVNE+DLDKM YLKA+
Sbjct: 306 MFAAGTETTSTVMEWTMSELLKHPEAMKKLKSEIREISGDHKAYYVNEDDLDKMHYLKAV 365

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           +KETLRLHPPIPLL+P+ES + I L+GYDIKP+TRV+ N       P++W+EAEEFR ER
Sbjct: 366 LKETLRLHPPIPLLVPRESTQCIKLKGYDIKPKTRVMINAWAIGRDPEVWDEAEEFRPER 425

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           FMNNSIDFKG DF LIPFG GRRGCPGI FATVINEI LANLVHKF+WM+ NGE LDMTG
Sbjct: 426 FMNNSIDFKGHDFDLIPFGAGRRGCPGIAFATVINEIALANLVHKFEWMLPNGEDLDMTG 485

Query: 181 AFGMTIHRKFPLVATTTPC 195
           AFG+TIHRK PLVAT TPC
Sbjct: 486 AFGLTIHRKHPLVATATPC 504

BLAST of Bhi02G000450 vs. ExPASy TrEMBL
Match: A0A5D3BJF9 (Cytochrome P450 71A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001080 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.3e-81
Identity = 148/199 (74.37%), Postives = 171/199 (85.93%), Query Frame = 0

Query: 1   MFAAGTDTTYTVLEWAMSKLLKHPKTMKKLKNEIRKIKGDHKGCYVNENDLDKMVYLKAI 60
           MFAAGTDT YTVLEWAMS+LLKHP+ MKKLKNEI +IKG+HKG Y+NE+D+D+MVYLKAI
Sbjct: 312 MFAAGTDTAYTVLEWAMSELLKHPEVMKKLKNEIGEIKGEHKGSYINEDDIDQMVYLKAI 371

Query: 61  IKETLRLHPPIPLLIPKESIKPINLRGYDIKPRTRVISNV-----APKLWEEAEEFRLER 120
           IKETLRLH PIPLL+P+ESIKP+ LRGYDIKP TRV+ N       PK+WEEAE+F+ ER
Sbjct: 372 IKETLRLHTPIPLLVPRESIKPVKLRGYDIKPGTRVMINAWTIGRDPKVWEEAEKFQPER 431

Query: 121 FMNNSIDFKGQDFVLIPFGVGRRGCPGIVFATVINEITLANLVHKFDWMVSNGEVLDMTG 180
           F+N+SIDFKGQDF LIPFG GRRGCPGI FAT + EI LANLVHKF+W++ NGE LDM+G
Sbjct: 432 FLNSSIDFKGQDFELIPFGAGRRGCPGIAFATTVIEIALANLVHKFEWILPNGEDLDMSG 491

Query: 181 AFGMTIHRKFPLVATTTPC 195
           A G+TIHRK PLVAT  PC
Sbjct: 492 ASGLTIHRKIPLVATAVPC 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G24960.16.6e-4948.02cytochrome P450, family 71, subfamily A, polypeptide 14 [more]
AT3G48280.11.9e-4846.80cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
AT3G48290.15.6e-4848.76cytochrome P450, family 71, subfamily A, polypeptide 24 [more]
AT3G48290.25.6e-4848.76cytochrome P450, family 71, subfamily A, polypeptide 24 [more]
AT3G48270.14.8e-4747.98cytochrome P450, family 71, subfamily A, polypeptide 26 [more]
Match NameE-valueIdentityDescription
A0A068Q6L24.0e-5956.22Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1[more]
P244651.5e-5355.38Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
O041641.5e-5353.23Cytochrome P450 71A6 (Fragment) OS=Nepeta racemosa OX=54731 GN=CYP71A6 PE=2 SV=1[more]
Q947B77.7e-5049.25(+)-menthofuran synthase OS=Mentha piperita OX=34256 PE=1 SV=1[more]
Q427162.9e-4950.00Cytochrome P450 71A8 OS=Mentha piperita OX=34256 GN=CYP71A8 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038880328.11.3e-107100.00cytochrome P450 736A117-like [Benincasa hispida][more]
XP_038880242.12.3e-9184.42cytochrome P450 736A117-like [Benincasa hispida][more]
XP_038880557.12.3e-9184.42cytochrome P450 736A117-like [Benincasa hispida][more]
XP_004138089.17.8e-8476.38cytochrome P450 71A22 [Cucumis sativus] >KGN63544.2 hypothetical protein Csa_013... [more]
XP_023515572.18.6e-8376.38cytochrome P450 71A24-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5A7UWH34.6e-8274.87Cytochrome P450 71A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A6J1E6T04.6e-8275.88cytochrome P450 71A3-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1114299... [more]
A0A6J1E4T46.0e-8275.38cytochrome P450 71A22-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429... [more]
A0A6J1E1I66.0e-8275.38cytochrome P450 71A3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114299... [more]
A0A5D3BJF92.3e-8174.37Cytochrome P450 71A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 58..76
score: 41.15
coord: 13..39
score: 32.95
coord: 140..163
score: 26.7
coord: 130..140
score: 54.13
coord: 94..118
score: 24.43
IPR001128Cytochrome P450PRINTSPR00385P450coord: 4..21
score: 40.41
coord: 131..140
score: 57.63
coord: 59..70
score: 48.31
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..175
e-value: 1.9E-49
score: 168.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..190
e-value: 8.8E-56
score: 191.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 1..193
NoneNo IPR availablePANTHERPTHR47956:SF4CYTOCHROME P450 71A21-RELATEDcoord: 1..192
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 1..192
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 133..142

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000450Bhi02M000450mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen