Bhi02G000427 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000427
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionHaloacid dehalogenase-like hydrolase domain-containing protein
Locationchr2: 10825219 .. 10828991 (+)
RNA-Seq ExpressionBhi02G000427
SyntenyBhi02G000427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAATCTGTGGTGCTCCGGTGGATAAGCTGTGTCGTAAAAGCCGTTAAAATGCTCAACTGAATTCTAACTTCACTAATTCACAAAAAAGCAACAGAGGAAGAAGAAGGAGACTGGATTCTAATGGCGTCGACTACACTCTCCGTCTCCTCGCCGTCGATTTCTGGTTCCACAACGCCTTGTCTTCCCAACACCACCACCACCGTCGCTTATTTCAAACCCCATACCAGAACTTTCTCTACTCTTCTCTTGGGCAAAAGCCTAAGCTTGTATCGAACATCCAGAATTCGGCCAATAGGCGCTAAGATTTCTTCTAATGGGATCACTTGCCAGGCTTCTTCTTCTGTTCTTCCCTCTGCTCTTCTTTTTGATTGCGATGGGGTTCTTGTCGATACTGAAAAGGATGGCCATCGGATTTCCTTCAATGAGACATTTGATGAGGTTTTTTTTCCCCTCTTTTCTTCGCTATTTCTTTTTTTGTTTATCTGCGTCTCTGACACTGCTTATTGATTTAGTGTTTACGGTAAAGTAGGGTTATATTTAGTACCTGGTGATGGTATAAACTCTAATGAACTGAATGGCATTTGGTTGATTAAATTTGTTTTCTGTTTCAATTGGTTGATTTAGGAGAATCAAGAAACATGGGCTTAGACTAGTTTTGATTTCTGGTTAAGCTATCCTAACGATATCATAATTGAAATGATTCCACAGAAAGAATTGGGCGTTACTTGGGATGTAGATTTGTATGGTGAGTTACTCAAAATTGGAGGTGGAAAAGAAAGGTAATTAACTTCTTGGCCTCAAAGATTTGATTAGCTGCGAGATCTTGATAATCTAAGTTCATTTGCGCTCACAGTAAGCGCTTGGTATTGGATTAAGGTTCTGTTTTCTAGTTGGAGTTCTTAGAAGAAAATGGTGGCAGATATCATATGATAATGATATTTGACTGTTGTAGGATGACAGCGTACTTCAATAAAGTTGGATGGCCAGAAAAGGCTCCAAAGAATGAAGATGAAAGAAAGGCATTCATTGCTGGACTTCACAAGCGAAAAACAGATTTGTTCATGGCCCTTATTGAGAAGCAATTGCTACCCCTTCGACCTGGTGTTGCAAAGTTAGTTACCTTACTAACTCCATTGACCTTGATGCTTATTGTAATAATGAGGCAATTAGGAAATGCAGAACCATGAAAAAAATACATTTAAAAAAAGGGATACATGATACTCATTGATATCATGTTAAGTCACTTAGGATTGAGATACATGATTTAAGCAATTGAATTCGTAAAGATTTTTTTGAAAAATTCCAAGTCAATATCTATCCCTCTGCTACCCGGTTTACGTGGTACTGTTCTCATTGACATAGTCCTCAGATTTCTCTGCTTTAAGCCTTTAACTGATCTACACTATGCAATTAAACTTCTCTTCAATTTTATCCCTTGGAAAATTCGGCACTGTCATATGTGTGATTGGTAATTTTAAAGTGAGTACAAGCACATGGACCTTTCTATGTTATTGAGAGAGGTGTATTTAAATTATGCAGGCTGATTGATCAGGCATTAGCAAAAGGAGTGAAAGTTGCTGTTTGTAGCACTTCCAATGAGAAGGCGGTATGCATTATCAGGTTCTATAATATTAGTCGCTTGTAGTTGTAATCTTCACGTATTCCTGCTATATTGAGTAGATTTTAATGTTATACAATTGTTTTGCTTATATAAAATTGTATACACTGCAAATAAAACTCTCTGTTGTTTGTTATAGATTAATTGAAATATTTTTTCTTACATCTGCTTATGATTGTAACAGCTGAACTTCGTCCATTCAATTGTAGGTCTCTGCGATAGTATCATGTTTACTGGGACCTCAACGAGCTGAGCAGATCAAGATATTTGCAGGAGATGTAGTGCCTCGAAAAAAGCCTGACCCAGTAAAATTTCTATCCCTCTCAACTCCCAAGTCTCTAGTGTTGATTGCTAAGATCAAGATATCTAGAGACGAACAATGAAGTTTACCTTGATTTTAGTTTCTTTATTATCAAAGTTTTTATTTTAACGTTAACTGTTAAAACGGAGAACTACTTTTTTCTATTTAACTCAGGTTAAATAAAATTTTTGACTAATTAATCTGTGCTCTGTTGCCACTAAATTGCCTTTCTCCATTGCAGGCAATCTATATTTTAGCAGCCAACACCCTGGGTGTTGATCCTTCAAGGTATGTCATGAATTAATCAGGAATGAGAATATTTAGGTCCATTTTAATAACTATTTAGACCCGTTTGAAAACCATTTGGTTTTTTAAAATTAAACTTATAAACACTAATTCTATCCATTGATTTCTTTGTTTTATTATATATTTTTTATGAGTGTTTTTCAAAACCAAGCCAAGTTTTGGAACCTAAAAAAATAGTTTTTAAAACTTTCTTTTGTTTTTGGAATTTGGTTAAAAAATCAAGTGATTAATTAGAAAGATGTAAATCATGATAAAAAATATGTAAGAAAACAAACATAATTTTCAAAAACTAAAAACCAAATGACTCAAATGAGGCCTTATTTTTTTTGTTTTGAAAATTATACTTCTTTCTTATAATTTCTTTATGATTGTCTTTATTTTTCTTGAGTTAACATATGAATTCATAGCTAAATTTTAAATTCCAAAAATTCTTTTTAAAAACTATTTTCTTTTGGTTTTAAATTTTGGATTGAACTTTAAAAATATCTCGTGACATTCAGAAGTAGTGTTTAAAAACTTGATTTCAAAAAATAAAATAACTAATAAATAGGACCTTAACGTTTCCATGGGCCTAGTATGTATGAAAATTTGATAGGATTACTTAAAAGGTATCTTTCTTTTATAAGCATAGTCACAAGGAACTTAATAAAATCGTATTGATGAACTAAAGAGTTTCTTTCTTTCCTTTTTTTTTTTTTTTTAAGTTTTTATTTCCATGACTTAATCTTTGTATAAATGGCATCCATGTGCAGAAAATTTATGCTCTTACTTTCTTTCTGCAGTTGTGTTGTGGTGGAGGACAGTGCCATTGGCCTTGCAGCTGCCAAAAGTGCAGGAATGAAGTGCATAGTAACAAAGAGCGGGTGAGTTAAGAAATTTTTTTGCACAAGATTATTCTTCAACTCTTTTGCATATGTGTAAAGAAAGTTTGTCAGATGAAGCTCAATACTTGTTTTGGTTCAGAATTGTATTCAGTTCATTTCTTTCACAAAGATCATAACTATTTTCTTCTAAAACACATACGAATCTACCTTCTTTTCTTCTTCAAGATAATATGTTTGAGTTAATCCGTGTTTCAGGTACACGGCTGAGGAAGATTTTGTAAATGCAGATGCTGTCTTTGACTGCATTGGAGATCCCCCAGAAGAGCGTTTTGACTTGGCATTCTGTGGAAGCCTTCTTGAGAAGCAGTATGTCAGCTAGACCATGCAAGCACCACTGCATAATTTTCACGTGCTGAAAACCGAAATATAGAGATGCCAGGTCATGCTTCTTGACTTAGATCATTGTATTTAAATCATGTATGCCAATTTGTTGATATATCATGTGTTTTCCCCTTGTAAATCAAGACGAGGGCGATTTCGTTTTATTTGAATTCTCATGCTTGACATGTTGCTGTTTCCTCCCAACCCCCTAGTTTTGCTTAATTTACTCGTAAATTTTTTTTCACCCGTAAGTTTAAATTTTTGGGTCCGTCGATAATTTAACACACACCCAAATTGTGACTATTTAGTTTTAGAACTTTCATAATTATTTTATTGTCCC

mRNA sequence

CACAAATCTGTGGTGCTCCGGTGGATAAGCTGTGTCGTAAAAGCCGTTAAAATGCTCAACTGAATTCTAACTTCACTAATTCACAAAAAAGCAACAGAGGAAGAAGAAGGAGACTGGATTCTAATGGCGTCGACTACACTCTCCGTCTCCTCGCCGTCGATTTCTGGTTCCACAACGCCTTGTCTTCCCAACACCACCACCACCGTCGCTTATTTCAAACCCCATACCAGAACTTTCTCTACTCTTCTCTTGGGCAAAAGCCTAAGCTTGTATCGAACATCCAGAATTCGGCCAATAGGCGCTAAGATTTCTTCTAATGGGATCACTTGCCAGGCTTCTTCTTCTGTTCTTCCCTCTGCTCTTCTTTTTGATTGCGATGGGGTTCTTGTCGATACTGAAAAGGATGGCCATCGGATTTCCTTCAATGAGACATTTGATGAGAAAGAATTGGGCGTTACTTGGGATGTAGATTTGTATGGTGAGTTACTCAAAATTGGAGGTGGAAAAGAAAGGATGACAGCGTACTTCAATAAAGTTGGATGGCCAGAAAAGGCTCCAAAGAATGAAGATGAAAGAAAGGCATTCATTGCTGGACTTCACAAGCGAAAAACAGATTTGTTCATGGCCCTTATTGAGAAGCAATTGCTACCCCTTCGACCTGGTGTTGCAAAGCTGATTGATCAGGCATTAGCAAAAGGAGTGAAAGTTGCTGTTTGTAGCACTTCCAATGAGAAGGCGGTCTCTGCGATAGTATCATGTTTACTGGGACCTCAACGAGCTGAGCAGATCAAGATATTTGCAGGAGATGTAGTGCCTCGAAAAAAGCCTGACCCAGCAATCTATATTTTAGCAGCCAACACCCTGGGTGTTGATCCTTCAAGTTGTGTTGTGGTGGAGGACAGTGCCATTGGCCTTGCAGCTGCCAAAAGTGCAGGAATGAAGTGCATAGTAACAAAGAGCGGGTACACGGCTGAGGAAGATTTTGTAAATGCAGATGCTGTCTTTGACTGCATTGGAGATCCCCCAGAAGAGCGTTTTGACTTGGCATTCTGTGGAAGCCTTCTTGAGAAGCAGTATGTCAGCTAGACCATGCAAGCACCACTGCATAATTTTCACGTGCTGAAAACCGAAATATAGAGATGCCAGGTCATGCTTCTTGACTTAGATCATTGTATTTAAATCATGTATGCCAATTTGTTGATATATCATGTGTTTTCCCCTTGTAAATCAAGACGAGGGCGATTTCGTTTTATTTGAATTCTCATGCTTGACATGTTGCTGTTTCCTCCCAACCCCCTAGTTTTGCTTAATTTACTCGTAAATTTTTTTTCACCCGTAAGTTTAAATTTTTGGGTCCGTCGATAATTTAACACACACCCAAATTGTGACTATTTAGTTTTAGAACTTTCATAATTATTTTATTGTCCC

Coding sequence (CDS)

ATGGCGTCGACTACACTCTCCGTCTCCTCGCCGTCGATTTCTGGTTCCACAACGCCTTGTCTTCCCAACACCACCACCACCGTCGCTTATTTCAAACCCCATACCAGAACTTTCTCTACTCTTCTCTTGGGCAAAAGCCTAAGCTTGTATCGAACATCCAGAATTCGGCCAATAGGCGCTAAGATTTCTTCTAATGGGATCACTTGCCAGGCTTCTTCTTCTGTTCTTCCCTCTGCTCTTCTTTTTGATTGCGATGGGGTTCTTGTCGATACTGAAAAGGATGGCCATCGGATTTCCTTCAATGAGACATTTGATGAGAAAGAATTGGGCGTTACTTGGGATGTAGATTTGTATGGTGAGTTACTCAAAATTGGAGGTGGAAAAGAAAGGATGACAGCGTACTTCAATAAAGTTGGATGGCCAGAAAAGGCTCCAAAGAATGAAGATGAAAGAAAGGCATTCATTGCTGGACTTCACAAGCGAAAAACAGATTTGTTCATGGCCCTTATTGAGAAGCAATTGCTACCCCTTCGACCTGGTGTTGCAAAGCTGATTGATCAGGCATTAGCAAAAGGAGTGAAAGTTGCTGTTTGTAGCACTTCCAATGAGAAGGCGGTCTCTGCGATAGTATCATGTTTACTGGGACCTCAACGAGCTGAGCAGATCAAGATATTTGCAGGAGATGTAGTGCCTCGAAAAAAGCCTGACCCAGCAATCTATATTTTAGCAGCCAACACCCTGGGTGTTGATCCTTCAAGTTGTGTTGTGGTGGAGGACAGTGCCATTGGCCTTGCAGCTGCCAAAAGTGCAGGAATGAAGTGCATAGTAACAAAGAGCGGGTACACGGCTGAGGAAGATTTTGTAAATGCAGATGCTGTCTTTGACTGCATTGGAGATCCCCCAGAAGAGCGTTTTGACTTGGCATTCTGTGGAAGCCTTCTTGAGAAGCAGTATGTCAGCTAG

Protein sequence

MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGAKISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIYILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDPPEERFDLAFCGSLLEKQYVS
Homology
BLAST of Bhi02G000427 vs. TAIR 10
Match: AT3G48420.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 453.0 bits (1164), Expect = 2.0e-127
Identity = 244/323 (75.54%), Postives = 267/323 (82.66%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPC-LPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIG 60
           +AS + S SS SI    +P  +PN     A  K     F+   L     +YR+SR     
Sbjct: 10  LASLSPSSSSSSIQSKLSPSFIPN----AAPAKAVKLRFNGKSLRAKPMVYRSSR----- 69

Query: 61  AKISSNGITCQASSSV--LPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDL 120
               S G+TC ASSS+  LPSALLFDCDGVLVDTEKDGHRISFN+TF E++L VTWDVDL
Sbjct: 70  ----SVGVTCSASSSLTTLPSALLFDCDGVLVDTEKDGHRISFNDTFKERDLNVTWDVDL 129

Query: 121 YGELLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPL 180
           YGELLKIGGGKERMTAYFNKVGWPEKAPK+E ERK FIAGLHK+KT+LFM LIEK+LLPL
Sbjct: 130 YGELLKIGGGKERMTAYFNKVGWPEKAPKDEAERKEFIAGLHKQKTELFMVLIEKKLLPL 189

Query: 181 RPGVAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDP 240
           RPGVAKL+DQAL  GVKVAVCSTSNEKAVSAIVSCLLGP+RAE+IKIFAGDVVP+KKPDP
Sbjct: 190 RPGVAKLVDQALTNGVKVAVCSTSNEKAVSAIVSCLLGPERAEKIKIFAGDVVPKKKPDP 249

Query: 241 AIYILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCI 300
           AIY LAA TLGVDPS CVVVEDSAIGLAAAK+AGM CIVTKSGYTA+EDF NADAVFDCI
Sbjct: 250 AIYNLAAETLGVDPSKCVVVEDSAIGLAAAKAAGMTCIVTKSGYTADEDFENADAVFDCI 309

Query: 301 GDPPEERFDLAFCGSLLEKQYVS 321
           GDPPEERFDLAFCGSLL KQ+VS
Sbjct: 310 GDPPEERFDLAFCGSLLRKQFVS 319

BLAST of Bhi02G000427 vs. TAIR 10
Match: AT4G39970.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.6e-39
Identity = 101/272 (37.13%), Postives = 157/272 (57.72%), Query Frame = 0

Query: 38  FSTLLLGKSLSLYRTSRIRPIGAKISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHR 97
           F TL   KS S+Y  SR+        S+ ++     S+   AL+FDCDGV++++E + HR
Sbjct: 35  FQTLRF-KSRSVYSKSRV--------SSPVSALPLRSL--EALIFDCDGVILESE-NLHR 94

Query: 98  ISFNETFDEKEL--------GVTWDVDLYGELLK-IGGGKERMTAYFNKVGWP-----EK 157
            ++N+ F   ++         + W ++ Y +    +GGGK +M  YF + GWP     + 
Sbjct: 95  QAYNDAFSHFDVRCPPSSSESLDWSLEFYDKFQNLVGGGKPKMRWYFKENGWPTSTIFDS 154

Query: 158 APKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVCSTSNE 217
            P+N+D+R   I  L   KT+ +  +I+   +  RPGV +L+D+A A G K+AVCS + +
Sbjct: 155 PPQNDDDRAKLIDTLQDWKTERYKEIIKSGSVEPRPGVIRLMDEAKAAGKKLAVCSAATK 214

Query: 218 KAVSAIVSCLLGPQRAEQIKIF-AGDVVPRKKPDPAIYILAANTLGVDPSSCVVVEDSAI 277
            +V   +  L+  +R + +  F AGD V  KKPDP+IYI AA  LGV    C+VVEDS I
Sbjct: 215 SSVILCLENLIDIERFQGLDCFLAGDDVKEKKPDPSIYITAAEKLGVSVKDCLVVEDSVI 274

Query: 278 GLAAAKSAGMKCIVTKSGYTAEEDFVNADAVF 295
           GL AA  AGM C++T +  T++++F +A AV+
Sbjct: 275 GLQAATKAGMSCVITYTSSTSDQNFNDAIAVY 294

BLAST of Bhi02G000427 vs. TAIR 10
Match: AT5G45170.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 5.0e-17
Identity = 85/324 (26.23%), Postives = 136/324 (41.98%), Query Frame = 0

Query: 42  LLGKSLSLYRTSRIRPIGAKISSNGITCQASS--SVLPS---ALLFDCDGVLVDTEKDGH 101
           +LGK L L R S I             C ++S   V PS   A++ + D V++DT    +
Sbjct: 51  ILGKCLRLQRFSSI-------------CLSASREDVNPSEEFAVILEVDRVMIDT-WSSN 110

Query: 102 RISFNETFDEKELG-VTWDVDLYGELLKIGGGKER--MTAYFNKVGWPEKAPKNEDERKA 161
           R +FN  F +  L    W   +Y +LL+ G   E   +  YFN++GWP   P +  E+ +
Sbjct: 111 RQAFNVAFQKLGLDCANWPEPVYSDLLRKGAADEEKMLLLYFNQIGWPSSLPTS--EKAS 170

Query: 162 FIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVCST---SNEKAVSAIV 221
           F+  + + K +     +  + LPLR GV + ID A A+ V VA+ +    S +K   +IV
Sbjct: 171 FVKSVLREKKNAMDEFLISKSLPLRSGVQEFIDNAYAEKVPVAIVTAYCKSGDKVALSIV 230

Query: 222 SCLLGPQRAEQIKIFAG------------------------------------------- 281
             +LG +R   +K+                                              
Sbjct: 231 E-MLGQERLPNVKVIGDNEVEQSMYGQLVLGKGVSSSLEEQLVKEVKKAASAEKQRIAEE 290

Query: 282 -----------DVVPRKKPDPAIYIL--AANTLGVDPSSCVVVEDSAIGLAAAKSAGMKC 299
                      D    ++ +  +  L  AA  +G+  ++CV+V  S  G++AAK  GM C
Sbjct: 291 VASMLKLSVDIDTTSSERLEKIVVALRAAAEHIGLPVNNCVLVAGSQPGVSAAKMIGMPC 350

BLAST of Bhi02G000427 vs. TAIR 10
Match: AT1G56500.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-10
Identity = 81/278 (29.14%), Postives = 124/278 (44.60%), Query Frame = 0

Query: 7   SVSSPSISGSTTPCLP-NTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGAKISSN 66
           SV S S   S++  +P  + +T   F+  T  +    L K+ +L  +++   +     S 
Sbjct: 10  SVFSQSRRLSSSSLIPIRSKSTFTGFRSRTGVY----LSKTTALQSSTK---LSVAAESP 69

Query: 67  GITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIG 126
             T         SA+LFD DGVL ++E D  R +  + F   E+GV   VD +   +  G
Sbjct: 70  AATIATDDWGKVSAVLFDMDGVLCNSE-DLSRRAAVDVF--TEMGVEVTVDDFVPFM--G 129

Query: 127 GGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLR----PGV 186
            G+ +       V    K  K  D   A        K   F   ++K   P      PG 
Sbjct: 130 TGEAKFLGGVASV----KEVKGFDPDAA--------KERFFEIYLDKYAKPESGIGFPGA 189

Query: 187 AKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIYI 246
            +L+ +   KG+KVAV S+++   V A +    G        I + D     KP P I++
Sbjct: 190 LELVTECKNKGLKVAVASSADRIKVDANLKA-AGLSLTMFDAIVSADAFENLKPAPDIFL 249

Query: 247 LAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKS 280
            AA  LGV  S CVV+ED+  G+ AA++A M+CI  K+
Sbjct: 250 AAAKILGVPTSECVVIEDALAGVQAAQAANMRCIAVKT 262

BLAST of Bhi02G000427 vs. TAIR 10
Match: AT4G21470.1 (riboflavin kinase/FMN hydrolase )

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-10
Identity = 72/232 (31.03%), Postives = 104/232 (44.83%), Query Frame = 0

Query: 76  LPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIGGGKERMTAYF 135
           L S +L D DG L++T  DG        +  K  G  WD     E LKI  GK  + A  
Sbjct: 10  LSSCVLIDLDGTLINT--DGVVGDILRKYLCK-YGKQWD---GRESLKI-VGKTPVEAAT 69

Query: 136 NKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKV 195
             V   E   K ++    F          LF A ++K  +   PG  +LI      GV V
Sbjct: 70  TIVEDYELPCKVDEFNSEFY--------PLFSAQMDK--IKSLPGANRLIRHLKCHGVPV 129

Query: 196 AVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIYILAANTLGVDPSSCV 255
           A+ S S+   + + +S   G +    + I   D V + KP P I++ AA  L  DP+ C+
Sbjct: 130 ALASNSSRANIESKISYHEGWKECFSV-IVGSDEVSKGKPSPDIFLEAAKRLKKDPADCL 189

Query: 256 VVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDPPEERFDL 308
           V+EDS  G+ A K+AG K I   S       + +AD V + + D   E++ L
Sbjct: 190 VIEDSVPGVMAGKAAGTKVIAVPSLPKQTHLYTSADEVINSLLDIRLEKWGL 223

BLAST of Bhi02G000427 vs. ExPASy Swiss-Prot
Match: Q94K71 (CBBY-like protein OS=Arabidopsis thaliana OX=3702 GN=CBBY PE=1 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 2.8e-126
Identity = 244/323 (75.54%), Postives = 267/323 (82.66%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPC-LPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIG 60
           +AS + S SS SI    +P  +PN     A  K     F+   L     +YR+SR     
Sbjct: 10  LASLSPSSSSSSIQSKLSPSFIPN----AAPAKAVKLRFNGKSLRAKPMVYRSSR----- 69

Query: 61  AKISSNGITCQASSSV--LPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDL 120
               S G+TC ASSS+  LPSALLFDCDGVLVDTEKDGHRISFN+TF E++L VTWDVDL
Sbjct: 70  ----SVGVTCSASSSLTTLPSALLFDCDGVLVDTEKDGHRISFNDTFKERDLNVTWDVDL 129

Query: 121 YGELLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPL 180
           YGELLKIGGGKERMTAYFNKVGWPEKAPK+E ERK FIAGLHK+KT+LFM LIEK+LLPL
Sbjct: 130 YGELLKIGGGKERMTAYFNKVGWPEKAPKDEAERKEFIAGLHKQKTELFMVLIEKKLLPL 189

Query: 181 RPGVAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDP 240
           RPGVAKL+DQAL  GVKVAVCSTSNEKAVSAIVSCLLGP+RAE+IKIFAGDVVP+KKPDP
Sbjct: 190 RPGVAKLVDQALTNGVKVAVCSTSNEKAVSAIVSCLLGPERAEKIKIFAGDVVPKKKPDP 249

Query: 241 AIYILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCI 300
           AIY LAA TLGVDPS CVVVEDSAIGLAAAK+AGM CIVTKSGYTA+EDF NADAVFDCI
Sbjct: 250 AIYNLAAETLGVDPSKCVVVEDSAIGLAAAKAAGMTCIVTKSGYTADEDFENADAVFDCI 309

Query: 301 GDPPEERFDLAFCGSLLEKQYVS 321
           GDPPEERFDLAFCGSLL KQ+VS
Sbjct: 310 GDPPEERFDLAFCGSLLRKQFVS 319

BLAST of Bhi02G000427 vs. ExPASy Swiss-Prot
Match: Q680K2 (Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970 OS=Arabidopsis thaliana OX=3702 GN=At4g39970 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.5e-38
Identity = 101/272 (37.13%), Postives = 157/272 (57.72%), Query Frame = 0

Query: 38  FSTLLLGKSLSLYRTSRIRPIGAKISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHR 97
           F TL   KS S+Y  SR+        S+ ++     S+   AL+FDCDGV++++E + HR
Sbjct: 35  FQTLRF-KSRSVYSKSRV--------SSPVSALPLRSL--EALIFDCDGVILESE-NLHR 94

Query: 98  ISFNETFDEKEL--------GVTWDVDLYGELLK-IGGGKERMTAYFNKVGWP-----EK 157
            ++N+ F   ++         + W ++ Y +    +GGGK +M  YF + GWP     + 
Sbjct: 95  QAYNDAFSHFDVRCPPSSSESLDWSLEFYDKFQNLVGGGKPKMRWYFKENGWPTSTIFDS 154

Query: 158 APKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVCSTSNE 217
            P+N+D+R   I  L   KT+ +  +I+   +  RPGV +L+D+A A G K+AVCS + +
Sbjct: 155 PPQNDDDRAKLIDTLQDWKTERYKEIIKSGSVEPRPGVIRLMDEAKAAGKKLAVCSAATK 214

Query: 218 KAVSAIVSCLLGPQRAEQIKIF-AGDVVPRKKPDPAIYILAANTLGVDPSSCVVVEDSAI 277
            +V   +  L+  +R + +  F AGD V  KKPDP+IYI AA  LGV    C+VVEDS I
Sbjct: 215 SSVILCLENLIDIERFQGLDCFLAGDDVKEKKPDPSIYITAAEKLGVSVKDCLVVEDSVI 274

Query: 278 GLAAAKSAGMKCIVTKSGYTAEEDFVNADAVF 295
           GL AA  AGM C++T +  T++++F +A AV+
Sbjct: 275 GLQAATKAGMSCVITYTSSTSDQNFNDAIAVY 294

BLAST of Bhi02G000427 vs. ExPASy Swiss-Prot
Match: P95649 (Protein CbbY OS=Rhodobacter sphaeroides OX=1063 GN=cbbY PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.7e-34
Identity = 92/223 (41.26%), Postives = 127/223 (56.95%), Query Frame = 0

Query: 79  ALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIGGGKERMTAYF-NK 138
           A+LFD DG L +TE + HR +FNETF    +   WD + Y ELL   GGKER+  +  ++
Sbjct: 4   AILFDVDGTLAETE-ELHRRAFNETFAALGVDWFWDREEYRELLTTTGGKERIARFLRHQ 63

Query: 139 VGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAV 198
            G P   P         IA +H+ KT+ F+AL+ +  + LRPG+A LI +A   G+++AV
Sbjct: 64  KGDPAPLP---------IADIHRAKTERFVALMAEGEIALRPGIADLIAEAKRAGIRLAV 123

Query: 199 CSTSNEKAVSAIV-SCLLGPQRAEQIKIFAGDVVPRKKPDPAIYILAANTLGVDPSSCVV 258
            +T++   V A+  +C   P R     I AGD+V  KKP P IY LA   L V P   V 
Sbjct: 124 ATTTSLPNVEALCRACFGHPAREIFDVIAAGDMVAEKKPSPDIYRLALRELDVPPERAVA 183

Query: 259 VEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGD 300
           +EDS  GL AAK AG++CIV+   YT  E+F  AD + D   +
Sbjct: 184 LEDSLNGLRAAKGAGLRCIVSPGFYTRHEEFAGADRLLDSFAE 216

BLAST of Bhi02G000427 vs. ExPASy Swiss-Prot
Match: O33513 (Protein CbbY OS=Rhodobacter capsulatus OX=1061 GN=cbbY PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.8e-33
Identity = 84/214 (39.25%), Postives = 127/214 (59.35%), Query Frame = 0

Query: 79  ALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIGGGKERMTAYFNKV 138
           AL+FD DG L +TE + HR +FNETF  + L   W  + Y  LL+  GGKERM  +   +
Sbjct: 5   ALIFDVDGTLAETE-EVHRQAFNETFAAQGLDWYWSKEDYRTLLRTTGGKERMAKHRENL 64

Query: 139 GWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVC 198
           G     P +     A IA LHK KT  ++ +I    + L PGVA+LID+A A G+++A+ 
Sbjct: 65  G---SGPSD-----AKIADLHKAKTQRYVEIIASGQVGLLPGVAELIDRAKASGLRLAIA 124

Query: 199 STSNEKAVSAIVSCLLGPQRAEQIKIF-AGDVVPRKKPDPAIYILAANTLGVDPSSCVVV 258
           +T+    V A+++        +  ++  AGD V +KKP P +Y+ A   LG+ P++C+  
Sbjct: 125 TTTTRANVDALIAATFSKPAGDIFEVIAAGDEVAQKKPAPDVYLRALQGLGLPPAACLAF 184

Query: 259 EDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNAD 292
           EDS  GLA+A++AG++ ++T S YT  +DF  AD
Sbjct: 185 EDSRAGLASARAAGLRVVLTPSEYTRGDDFSAAD 209

BLAST of Bhi02G000427 vs. ExPASy Swiss-Prot
Match: P40119 (Protein CbbY, chromosomal OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) OX=381666 GN=cbbYC PE=3 SV=3)

HSP 1 Score: 134.4 bits (337), Expect = 2.2e-30
Identity = 85/225 (37.78%), Postives = 122/225 (54.22%), Query Frame = 0

Query: 79  ALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGELLKIGGGKERMTAYFNKV 138
           AL+FD DG L DTE   H  +FN  F E  L   WD  LY  LLK+ GGKER+  Y+  V
Sbjct: 3   ALIFDVDGTLADTE-SAHLQAFNAAFAEVGLDWYWDAPLYTRLLKVAGGKERLMHYWRMV 62

Query: 139 GWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPGVAKLIDQALAKGVKVAVC 198
             PE+A     + K  I  +H  KT  +   +    LPLRPG+A+LID+A   G+ +A+ 
Sbjct: 63  D-PEEA--RGCKVKETIDAVHAIKTRHYAERVGAGGLPLRPGIARLIDEAGEAGLPLAIA 122

Query: 199 STSNEKAVSAIVSCLLGPQ-RAEQIKIFAGDVVPRKKPDPAIYILAANTLGVDPSSCVVV 258
           +T+    + A++   LG   R     I        KKP P +Y+     LG++   C+ +
Sbjct: 123 TTTTPANLDALLQAPLGADWRRRFAAIGDAGTTAIKKPAPDVYLAVLERLGLEGGDCLAI 182

Query: 259 EDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDPPE 303
           EDSA GL AA++AG+  +VT + ++A++ F  A  V   +GDP E
Sbjct: 183 EDSANGLRAARAAGIPTVVTPTAFSAQDSFEGALLVLPHLGDPGE 223

BLAST of Bhi02G000427 vs. NCBI nr
Match: XP_038878915.1 (CBBY-like protein [Benincasa hispida])

HSP 1 Score: 624.0 bits (1608), Expect = 7.1e-175
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA
Sbjct: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE
Sbjct: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 320

BLAST of Bhi02G000427 vs. NCBI nr
Match: KAA0057796.1 (haloacid dehalogenase-like hydrolase domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 584.7 bits (1506), Expect = 4.8e-163
Identity = 302/320 (94.38%), Postives = 311/320 (97.19%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+T TTVAYFKPH R+FST LLGKSLSLYRTSRIRPI A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHTATTVAYFKPHNRSFSTSLLGKSLSLYRTSRIRPIDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K + NGITCQA SSVLPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNAPNGITCQA-SSVLPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVS LLG QRAEQ+KIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQMKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. NCBI nr
Match: XP_008464480.1 (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein At3g48420 [Cucumis melo] >TYJ98480.1 haloacid dehalogenase-like hydrolase domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 582.4 bits (1500), Expect = 2.4e-162
Identity = 301/320 (94.06%), Postives = 310/320 (96.88%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+  TTVAYFKPH R+FST LLGKSLSLYRTSRIRPI A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHIATTVAYFKPHNRSFSTSLLGKSLSLYRTSRIRPIDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K + NGITCQA SSVLPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNAPNGITCQA-SSVLPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVS LLG QRAEQ+KIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQMKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. NCBI nr
Match: XP_004138069.1 (CBBY-like protein [Cucumis sativus] >KGN63509.1 hypothetical protein Csa_014138 [Cucumis sativus])

HSP 1 Score: 579.7 bits (1493), Expect = 1.5e-161
Identity = 299/320 (93.44%), Postives = 308/320 (96.25%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+T TTVAYFKPH R+FST LLGKSLSLY TSRIRP  A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHTATTVAYFKPHNRSFSTSLLGKSLSLYPTSRIRPTDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K +SNGITCQA SS+LPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNASNGITCQA-SSILPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQAL KGVKVAVCSTSNEKAVSAIVS LLG QRAEQIKIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALGKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQIKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDL FCGSLLEKQYVS
Sbjct: 301 PEERFDLTFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. NCBI nr
Match: XP_022924807.1 (CBBY-like protein [Cucurbita moschata])

HSP 1 Score: 567.0 bits (1460), Expect = 1.0e-157
Identity = 294/320 (91.88%), Postives = 302/320 (94.38%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLSVSS SIS S TPCLPN +TTVAYFKP+T + ST ++GK + LYR+SR RPIGA
Sbjct: 1   MASTTLSVSSASISTSKTPCLPN-STTVAYFKPNTTSLSTSIVGKRIRLYRSSRTRPIGA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K +S GI CQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETF EKELGVTWDVDLYGE
Sbjct: 61  KNASTGINCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFQEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPKNEDERK FIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKTFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQAL  GVKVAVCSTSNEKAVSAIVS LLGPQRAEQIKIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALGNGVKVAVCSTSNEKAVSAIVSFLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. ExPASy TrEMBL
Match: A0A5A7UUG3 (Haloacid dehalogenase-like hydrolase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00790 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.3e-163
Identity = 302/320 (94.38%), Postives = 311/320 (97.19%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+T TTVAYFKPH R+FST LLGKSLSLYRTSRIRPI A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHTATTVAYFKPHNRSFSTSLLGKSLSLYRTSRIRPIDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K + NGITCQA SSVLPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNAPNGITCQA-SSVLPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVS LLG QRAEQ+KIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQMKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. ExPASy TrEMBL
Match: A0A5D3BFA5 (Haloacid dehalogenase-like hydrolase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00820 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 301/320 (94.06%), Postives = 310/320 (96.88%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+  TTVAYFKPH R+FST LLGKSLSLYRTSRIRPI A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHIATTVAYFKPHNRSFSTSLLGKSLSLYRTSRIRPIDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K + NGITCQA SSVLPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNAPNGITCQA-SSVLPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVS LLG QRAEQ+KIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQMKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. ExPASy TrEMBL
Match: A0A1S3CN37 (haloacid dehalogenase-like hydrolase domain-containing protein At3g48420 OS=Cucumis melo OX=3656 GN=LOC103502348 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 301/320 (94.06%), Postives = 310/320 (96.88%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+  TTVAYFKPH R+FST LLGKSLSLYRTSRIRPI A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHIATTVAYFKPHNRSFSTSLLGKSLSLYRTSRIRPIDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K + NGITCQA SSVLPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNAPNGITCQA-SSVLPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQALAKGVKVAVCSTSNEKAVSAIVS LLG QRAEQ+KIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQMKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. ExPASy TrEMBL
Match: A0A0A0LRC0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002780 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.4e-162
Identity = 299/320 (93.44%), Postives = 308/320 (96.25%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLS+SSPSISGSTTPCLP+T TTVAYFKPH R+FST LLGKSLSLY TSRIRP  A
Sbjct: 1   MASTTLSLSSPSISGSTTPCLPHTATTVAYFKPHNRSFSTSLLGKSLSLYPTSRIRPTDA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K +SNGITCQA SS+LPSALLFDCDGVLVDTEKDGHRISFNETF+EKELGVTWDVDLYGE
Sbjct: 61  KNASNGITCQA-SSILPSALLFDCDGVLVDTEKDGHRISFNETFEEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPK+EDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKDEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQAL KGVKVAVCSTSNEKAVSAIVS LLG QRAEQIKIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALGKGVKVAVCSTSNEKAVSAIVSFLLGSQRAEQIKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAK+AGMKCIVTKSGYTAEEDF+NADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKAAGMKCIVTKSGYTAEEDFLNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDL FCGSLLEKQYVS
Sbjct: 301 PEERFDLTFCGSLLEKQYVS 319

BLAST of Bhi02G000427 vs. ExPASy TrEMBL
Match: A0A6J1EA15 (CBBY-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432189 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.0e-158
Identity = 294/320 (91.88%), Postives = 302/320 (94.38%), Query Frame = 0

Query: 1   MASTTLSVSSPSISGSTTPCLPNTTTTVAYFKPHTRTFSTLLLGKSLSLYRTSRIRPIGA 60
           MASTTLSVSS SIS S TPCLPN +TTVAYFKP+T + ST ++GK + LYR+SR RPIGA
Sbjct: 1   MASTTLSVSSASISTSKTPCLPN-STTVAYFKPNTTSLSTSIVGKRIRLYRSSRTRPIGA 60

Query: 61  KISSNGITCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFDEKELGVTWDVDLYGE 120
           K +S GI CQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETF EKELGVTWDVDLYGE
Sbjct: 61  KNASTGINCQASSSVLPSALLFDCDGVLVDTEKDGHRISFNETFQEKELGVTWDVDLYGE 120

Query: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKAFIAGLHKRKTDLFMALIEKQLLPLRPG 180
           LLKIGGGKERMTAYFNKVGWPEKAPKNEDERK FIAGLHKRKTDLFMALIEKQLLPLRPG
Sbjct: 121 LLKIGGGKERMTAYFNKVGWPEKAPKNEDERKTFIAGLHKRKTDLFMALIEKQLLPLRPG 180

Query: 181 VAKLIDQALAKGVKVAVCSTSNEKAVSAIVSCLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240
           VAKLIDQAL  GVKVAVCSTSNEKAVSAIVS LLGPQRAEQIKIFAGDVVPRKKPDPAIY
Sbjct: 181 VAKLIDQALGNGVKVAVCSTSNEKAVSAIVSFLLGPQRAEQIKIFAGDVVPRKKPDPAIY 240

Query: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300
           ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP
Sbjct: 241 ILAANTLGVDPSSCVVVEDSAIGLAAAKSAGMKCIVTKSGYTAEEDFVNADAVFDCIGDP 300

Query: 301 PEERFDLAFCGSLLEKQYVS 321
           PEERFDLAFCGSLLEKQYVS
Sbjct: 301 PEERFDLAFCGSLLEKQYVS 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G48420.12.0e-12775.54Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT4G39970.14.6e-3937.13Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G45170.15.0e-1726.23Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT1G56500.11.5e-1029.14haloacid dehalogenase-like hydrolase family protein [more]
AT4G21470.11.5e-1031.03riboflavin kinase/FMN hydrolase [more]
Match NameE-valueIdentityDescription
Q94K712.8e-12675.54CBBY-like protein OS=Arabidopsis thaliana OX=3702 GN=CBBY PE=1 SV=1[more]
Q680K26.5e-3837.13Haloacid dehalogenase-like hydrolase domain-containing protein At4g39970 OS=Arab... [more]
P956495.7e-3441.26Protein CbbY OS=Rhodobacter sphaeroides OX=1063 GN=cbbY PE=1 SV=1[more]
O335134.8e-3339.25Protein CbbY OS=Rhodobacter capsulatus OX=1061 GN=cbbY PE=3 SV=1[more]
P401192.2e-3037.78Protein CbbY, chromosomal OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / ... [more]
Match NameE-valueIdentityDescription
XP_038878915.17.1e-175100.00CBBY-like protein [Benincasa hispida][more]
KAA0057796.14.8e-16394.38haloacid dehalogenase-like hydrolase domain-containing protein [Cucumis melo var... [more]
XP_008464480.12.4e-16294.06PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein At3g48... [more]
XP_004138069.11.5e-16193.44CBBY-like protein [Cucumis sativus] >KGN63509.1 hypothetical protein Csa_014138 ... [more]
XP_022924807.11.0e-15791.88CBBY-like protein [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5A7UUG32.3e-16394.38Haloacid dehalogenase-like hydrolase domain-containing protein OS=Cucumis melo v... [more]
A0A5D3BFA51.1e-16294.06Haloacid dehalogenase-like hydrolase domain-containing protein OS=Cucumis melo v... [more]
A0A1S3CN371.1e-16294.06haloacid dehalogenase-like hydrolase domain-containing protein At3g48420 OS=Cucu... [more]
A0A0A0LRC07.4e-16293.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002780 PE=4 SV=1[more]
A0A6J1EA155.0e-15891.88CBBY-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432189 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041492Haloacid dehalogenase-like hydrolasePFAMPF13419HAD_2coord: 80..276
e-value: 8.2E-19
score: 68.4
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01509TIGR01509coord: 163..276
e-value: 4.7E-17
score: 60.6
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 79..296
e-value: 5.9E-97
score: 325.7
IPR023198Phosphoglycolate phosphatase-like, domain 2GENE3D1.10.150.240Putative phosphatase; domain 2coord: 91..175
e-value: 5.9E-97
score: 325.7
NoneNo IPR availableSFLDSFLDF00035phosphoglycolate_phosphatasecoord: 76..303
e-value: 0.0
score: 337.4
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 76..303
e-value: 0.0
score: 337.4
NoneNo IPR availablePANTHERPTHR42896:SF2CBBY-LIKE PROTEINcoord: 66..319
NoneNo IPR availableCDDcd07528HAD_CbbY-likecoord: 79..279
e-value: 7.5521E-102
score: 295.443
IPR044999Protein CbbY-likePANTHERPTHR42896XYLULOSE-1,5-BISPHOSPHATE (XUBP) PHOSPHATASEcoord: 66..319
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 78..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000427Bhi02M000427mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity