Bhi02G000371 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000371
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic
Location	chr2: 9475674 .. 9476954 (-)
RNA-Seq Expression	Bhi02G000371
Synteny	Bhi02G000371

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGAAACAGCAGCCAAGACAGGAATCGAAGGAAGGATTATCAACGTTTCTTCAGTTGTCCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCGAACAAGTACGCCAACATTTTCTACAGTCTATTTGGAATACAAATACTTTTGCACTTAAAAATACTCAGGGAGCAATTAATGGAGTTATTTAGTAAGTTGGCATTGCTAAATTTGGGGAGTTGTTTAATCGTAGATCTACCATGCAATTTTTTTTTTTTTAATTTTTAAGTTGATAGTACAATCACTGTGCTAATTAAACTATGTTCATTTTGGTCAGTGTAAAGATATTACATGAAACTTAGGTTTAAGAACTCCACTCTCCCCAACTTCTATTCCTTGCATTGGACATGCTTTTAAAAAGTCATTTCAAACCGGCAGTCTCATTTTCTTTAATACTGTTATTGTGAAAGAAATATTGAAGAATTGTGTTTTATTTGCAGTTACAATGGCACTCGTGCATACGCTCAGTCAAAACTGGCCAACATTTTGCATGCTAAGGAAATATCAAGACAACTTCAGGTAGAGTTTTCTAAAACATCATTGCAAATCCTTCCTTATTCCTTAAAAGAACCCTCAACCCTATTCCTCAAAGTATCCATTTCCTTCTAATTCTTCCAATACCCAGCTACTAGCTAACAATTGCAAGAAATATTAAGCAAGGAAAAGATTTCAGTGACTCACCAAGGTTCTTTTTTGCGGCATGGCAGGGAAGAAATGCCAGAGTCACCATAAATGCAGTACATCCAGGAATTGTAAAAACTGCTATCATTAGAGCTCACAAGGGCATTATCACAGGTAGACGCTATAACTTCAGCGTTAAATTGTTCTTATGCATATGAATAACGTAAAGTTCGTCCTTCAACACAAGCATGAGAGAAACTGACTTCTTAAACTTTCCTGTGCAGATTCTTTGTTTTTTATGGCATCAAAGTTGCTAAAAACTACAAGTCAGGTATGATTCATCAGAAGCAAATAATCTTCCCCCCCCCCAAAGCACTGTTCATGATTGATTTAACTCACAAAATACACAAAATGGCTATGCAGGGAGCATCAACAACATGCTATGTAGCACTGAGTTCGCAAACAGAAGGAAAGAGTGGAAAATTTTACGCAGATTGTAACGAGACTACCTGCTCATCCCTAGCCAATGATGAGTTAGAAGCACAGAAGTTTTGGACTCAAACTCACAACTTAATCAATAGACGACTAAGCAAACTTTCCTCTTAA

mRNA sequence

ATGATAGAAACAGCAGCCAAGACAGGAATCGAAGGAAGGATTATCAACGTTTCTTCAGTTGTCCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCGAACAATTACAATGGCACTCGTGCATACGCTCAGTCAAAACTGGCCAACATTTTGCATGCTAAGGAAATATCAAGACAACTTCAGGGAAGAAATGCCAGAGTCACCATAAATGCAGTACATCCAGGAATTGTAAAAACTGCTATCATTAGAGCTCACAAGGGCATTATCACAGATTCTTTGTTTTTTATGGCATCAAAGTTGCTAAAAACTACAAGTCAGGGAGCATCAACAACATGCTATGTAGCACTGAGTTCGCAAACAGAAGGAAAGAGTGGAAAATTTTACGCAGATTGTAACGAGACTACCTGCTCATCCCTAGCCAATGATGAGTTAGAAGCACAGAAGTTTTGGACTCAAACTCACAACTTAATCAATAGACGACTAAGCAAACTTTCCTCTTAA

Coding sequence (CDS)

ATGATAGAAACAGCAGCCAAGACAGGAATCGAAGGAAGGATTATCAACGTTTCTTCAGTTGTCCATGGCTGGGTGAAGAAAGATGGTTTAAGCTTTCGTCAAATGCTCAACCCGAACAATTACAATGGCACTCGTGCATACGCTCAGTCAAAACTGGCCAACATTTTGCATGCTAAGGAAATATCAAGACAACTTCAGGGAAGAAATGCCAGAGTCACCATAAATGCAGTACATCCAGGAATTGTAAAAACTGCTATCATTAGAGCTCACAAGGGCATTATCACAGATTCTTTGTTTTTTATGGCATCAAAGTTGCTAAAAACTACAAGTCAGGGAGCATCAACAACATGCTATGTAGCACTGAGTTCGCAAACAGAAGGAAAGAGTGGAAAATTTTACGCAGATTGTAACGAGACTACCTGCTCATCCCTAGCCAATGATGAGTTAGAAGCACAGAAGTTTTGGACTCAAACTCACAACTTAATCAATAGACGACTAAGCAAACTTTCCTCTTAA

Protein sequence

MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKEISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS

Homology

[Close]

BLAST of Bhi02G000371 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 7.4e-60
Identity = 117/167 (70.06%), Postives = 139/167 (83.23%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNP-NNYNGTRAYAQSKLANILHAK 60
           MI+TA K+GIEGRIIN+SSV+H WVK D  SF ++L+P + YNGTRAYAQSKLA ILHAK
Sbjct: 159 MIDTAEKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAK 218

Query: 61  EISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYV 120
            +S+QL+ RNA VTINAVHPGIVKT IIRAHKG+ TDSLF +ASKLLK+ SQGA+TTCYV
Sbjct: 219 ALSKQLKDRNANVTINAVHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYV 278

Query: 121 ALSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRL 167
           ALS++T+G SGK++ADCNET CS LANDE  A K  TQ+  LI+  L
Sbjct: 279 ALSNETKGLSGKYFADCNETNCSDLANDEYVALKLCTQSRALIHDHL 325

[Close]

BLAST of Bhi02G000371 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-41
Identity = 84/165 (50.91%), Postives = 114/165 (69.09%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN--NYNGTRAYAQSKLANILHA 60
           MIETAA+TG++GRI+NV+SVVH W   D L +   ++ N  NY+ TRAYA SKLAN+LH 
Sbjct: 157 MIETAAQTGVQGRIVNVTSVVHSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHT 216

Query: 61  KEISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCY 120
            E+SR L   +A VT N VHPGIVKT + R  +G++TD +FF+ SKLLK+  Q A+TTCY
Sbjct: 217 VELSRLLHKMDANVTANCVHPGIVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCY 276

Query: 121 VALSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLIN 164
           VA S +     GK+++DCNE   S   +  L+AQ+ WT +  L++
Sbjct: 277 VATSPRLRNVCGKYFSDCNEARSSKSGSCNLKAQRLWTASDLLVS 321

[Close]

BLAST of Bhi02G000371 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.3e-40
Identity = 79/166 (47.59%), Postives = 116/166 (69.88%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN--NYNGTRAYAQSKLANILHA 60
           MI+TA +TG++GRI+NV+S +HGW   D + + ++++     ++ TRAYA SKLAN+LH 
Sbjct: 157 MIQTAEETGVQGRIVNVTSGIHGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHT 216

Query: 61  KEISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCY 120
           KE+S +LQ   A VT+N VHPG+V+T + R  +G++TD +FF+ASKL+KT  Q A+TTCY
Sbjct: 217 KELSSRLQKIGANVTVNCVHPGVVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCY 276

Query: 121 VALSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINR 165
           VA + +    SGK++ DCNETT S L  +  EA K W  +  L+ +
Sbjct: 277 VATNPRLVNVSGKYFTDCNETTPSGLGTNSSEATKLWAASEILVTQ 322

[Close]

BLAST of Bhi02G000371 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.1e-39
Identity = 82/112 (73.21%), Postives = 96/112 (85.71%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNP-NNYNGTRAYAQSKLANILHAK 60
           MI+TA K+GIEGRIIN+SSV+H WVK D  SF ++L+P + YNGTRAYAQSKLA ILHAK
Sbjct: 159 MIDTAEKSGIEGRIINLSSVIHNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAK 218

Query: 61  EISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQ 112
            +S+QL+ RNA VTINAVHPGIVKT IIRAHKG+ TDSLF +ASKLLK+ SQ
Sbjct: 219 ALSKQLKDRNANVTINAVHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

[Close]

BLAST of Bhi02G000371 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 7.4e-36
Identity = 79/163 (48.47%), Postives = 106/163 (65.03%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           M  TA  +G+EGRI+NVSSV H +  ++G+ F  + +  +Y+  RAY QSKLANILHA E
Sbjct: 156 MKNTAKTSGVEGRILNVSSVAHIYTYQEGIQFDSINDICSYSDKRAYGQSKLANILHANE 215

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQ     +T N+VHPG++ T + + H  ++   L F +  L K   QGA+TTCYVA
Sbjct: 216 LSRQLQEEGVNITANSVHPGLILTNLFQ-HTALLMRFLKFFSFYLWKNIPQGAATTCYVA 275

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLIN 164
           L    +G +GK++ADCNE T S LA DE  AQK W  +  LIN
Sbjct: 276 LHPSVKGVTGKYFADCNEVTPSKLARDETLAQKLWDFSVKLIN 317

[Close]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.2e-30
Identity = 70/163 (42.94%), Postives = 104/163 (63.80%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           M  +A ++GIEGRI+N+SS+ H +   +G+ F  + +P+ Y+  +AY QSKLAN+LH+  
Sbjct: 156 MKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNA 215

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SR+LQ     +TIN+VHPG++ T + R H G+    L  M+  L K   QGA+TTCYVA
Sbjct: 216 LSRKLQEEGVNITINSVHPGLITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVA 275

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLIN 164
           L    +G +GK++ DCN TT S+ A D   A K W  +  L++
Sbjct: 276 LHPDLKGVTGKYFTDCNVTTPSNFAIDTTLADKLWDFSIKLVD 317

[Close]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.5e-30
Identity = 70/162 (43.21%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           M  +A ++GIEGRI+N+SS+ H +   +G+ F  + +P+ Y+  +AY QSKLAN+LH+  
Sbjct: 156 MKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDYINDPDRYSEKKAYGQSKLANLLHSNA 215

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SR+LQ     +TIN+VHPG++ T + R H G+    L  M+  L K   QGA+TTCYVA
Sbjct: 216 LSRKLQEEGVNITINSVHPGLITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVA 275

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLI 163
           L    +  +GK++ADCN TT S+ A D   A K W  +  L+
Sbjct: 276 LHPDLKDVTGKYFADCNVTTPSNFAIDTTLADKLWDFSIKLV 316

[Close]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-24
Identity = 59/165 (35.76%), Postives = 99/165 (60.00%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           M +T  ++  EGRI+NV+S  H +   +G+ F ++ + ++YN  RAY QSKLAN+LHA +
Sbjct: 153 MKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKINDQSSYNNWRAYGQSKLANVLHANQ 212

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +++ L+     +T N++HPG + T + R H   +   +  +   +LK   QGA+TTCYVA
Sbjct: 213 LTKHLKEDGVNITANSLHPGTIVTNLFR-HNSAVNGLINVIGKLVLKNVQQGAATTCYVA 272

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRR 166
           L  Q +G SG++++D N    +    D   A+K W  + NL+ ++
Sbjct: 273 LHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLWDFSINLVKQK 316

[Close]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 61/171 (35.67%), Postives = 101/171 (59.06%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           M  T+ ++  EGRI+N+SS  H +   +G+ F ++ + ++Y+  RAY QSKL N+LHA E
Sbjct: 152 MKSTSRESKREGRIVNLSSEAHRFSYPEGVRFDKINDKSSYSSMRAYGQSKLCNVLHANE 211

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +++QL+     +T N++HPG + T + R     +  ++  +A  +LK+  QGA+TTCYVA
Sbjct: 212 LTKQLKEDGVNITANSLHPGAIMTNLGRYFNPYLAVAVGAVAKYILKSVPQGAATTCYVA 271

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           L+ Q  G SG+++ D N      L  D   A+K W  +  L + +  + SS
Sbjct: 272 LNPQVAGVSGEYFQDSNIAKPLPLVKDTELAKKVWDFSTKLTDSQSGESSS 322

[Close]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-18
Identity = 58/151 (38.41%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 7   KTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKEISRQLQ 66
           K+    RI+ VSS ++   K   ++F  + +  +YN +  Y++SKLANIL  +E++R+L+
Sbjct: 180 KSSAPSRIVVVSSKLY---KYGDINFDDLNSEQSYNKSFCYSRSKLANILFTRELARRLE 239

Query: 67  GRNARVTINAVHPGIVKTAIIR-AHKGIITDSLFFMAS-KLLKTTSQGASTTCYVALSSQ 126
           G N  VT+N +HPGIV+T + R  H  ++   LF + S    KT  +GA T+ Y+A S +
Sbjct: 240 GTN--VTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWAFFKTPVEGAQTSIYLASSPE 299

Query: 127 TEGKSGKFYADCNETTCSSLANDELEAQKFW 156
            EG SG+++ DC E      A DE  A+K W
Sbjct: 300 VEGVSGRYFGDCKEEELLPKAMDESVARKLW 325

[Close]

BLAST of Bhi02G000371 vs. NCBI nr
Match: XP_004138098.3 (short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN63560.2 hypothetical protein Csa_013220 [Cucumis sativus])

HSP 1 Score: 320.9 bits (821), Expect = 6.9e-84
Identity = 164/171 (95.91%), Postives = 166/171 (97.08%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN+YNGTRAYAQSKLANILHAKE
Sbjct: 187 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKE 246

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 247 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 306

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 307 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 357

[Close]

BLAST of Bhi02G000371 vs. NCBI nr
Match: TYJ98519.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 320.5 bits (820), Expect = 9.0e-84
Identity = 164/171 (95.91%), Postives = 165/171 (96.49%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKE
Sbjct: 160 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKE 219

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 220 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 279

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 280 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 330

[Close]

BLAST of Bhi02G000371 vs. NCBI nr
Match: XP_008464537.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo])

HSP 1 Score: 320.5 bits (820), Expect = 9.0e-84
Identity = 164/171 (95.91%), Postives = 165/171 (96.49%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKE
Sbjct: 193 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKE 252

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 253 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 312

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 313 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 363

[Close]

BLAST of Bhi02G000371 vs. NCBI nr
Match: KAG7023113.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 317.0 bits (811), Expect = 9.9e-83
Identity = 161/171 (94.15%), Postives = 166/171 (97.08%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSF QMLNPNNYNGTRAYAQSKLANILHAKE
Sbjct: 174 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKE 233

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 234 VSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 293

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WT+T NLIN++LSKLS+
Sbjct: 294 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 344

[Close]

BLAST of Bhi02G000371 vs. NCBI nr
Match: XP_023516084.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.0 bits (811), Expect = 9.9e-83
Identity = 161/171 (94.15%), Postives = 166/171 (97.08%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSF QMLNPNNYNGTRAYAQSKLANILHAKE
Sbjct: 176 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKE 235

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 236 VSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 295

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WT+T NLIN++LSKLS+
Sbjct: 296 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTRTRNLINKQLSKLST 346

[Close]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL
Match: A0A0A0LNV3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G004220 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.3e-84
Identity = 164/171 (95.91%), Postives = 166/171 (97.08%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN+YNGTRAYAQSKLANILHAKE
Sbjct: 172 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNSYNGTRAYAQSKLANILHAKE 231

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 232 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 291

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 292 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 342

[Close]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL
Match: A0A5D3BHM5 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001240 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.3e-84
Identity = 164/171 (95.91%), Postives = 165/171 (96.49%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKE
Sbjct: 160 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKE 219

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 220 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 279

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 280 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 330

[Close]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL
Match: A0A1S3CLP7 (short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502388 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.3e-84
Identity = 164/171 (95.91%), Postives = 165/171 (96.49%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKE
Sbjct: 193 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKE 252

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 253 LSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 312

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 313 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 363

[Close]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL
Match: A0A5A7UW68 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001220 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.8e-82
Identity = 162/171 (94.74%), Postives = 163/171 (95.32%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPN YNGTRAYAQSKLANILHAKE
Sbjct: 158 MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNRYNGTRAYAQSKLANILHAKE 217

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQ  NARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 218 LSRQLQVENARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 277

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDELEAQK WTQT NLINRRLSKL S
Sbjct: 278 LSSQTEGKSGKFYADCNETNCSSLANDELEAQKLWTQTRNLINRRLSKLPS 328

[Close]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL
Match: A0A6J1JH35 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485650 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.4e-82
Identity = 159/171 (92.98%), Postives = 166/171 (97.08%), Query Frame = 0


Query: 1   MIETAAKTGIEGRIINVSSVVHGWVKKDGLSFRQMLNPNNYNGTRAYAQSKLANILHAKE 60
           MIETAA+TGIEGRIINVSSVVHGWVKKDGLSF QMLNPNNYNGTRAYAQSKLANILHAKE
Sbjct: 172 MIETAAQTGIEGRIINVSSVVHGWVKKDGLSFGQMLNPNNYNGTRAYAQSKLANILHAKE 231

Query: 61  ISRQLQGRNARVTINAVHPGIVKTAIIRAHKGIITDSLFFMASKLLKTTSQGASTTCYVA 120
           +SRQLQGRNARVTINAVHPGIVKTAIIRAHKG ITDSLFFMASKLLKTTSQGASTTCYVA
Sbjct: 232 VSRQLQGRNARVTINAVHPGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVA 291

Query: 121 LSSQTEGKSGKFYADCNETTCSSLANDELEAQKFWTQTHNLINRRLSKLSS 172
           LSSQTEGKSGKFYADCNET CSSLANDE+EAQK WT+T NLIN++LSKLS+
Sbjct: 292 LSSQTEGKSGKFYADCNETNCSSLANDEIEAQKLWTRTRNLINKQLSKLST 342

The following BLAST results are available for this feature:

BLAST of Bhi02G000371 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50130.1	7.4e-60	70.06	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.0e-41	50.91	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24050.1	1.3e-40	47.59	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	1.1e-39	73.21	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	7.4e-36	48.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of Bhi02G000371 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078ISJ6	1.2e-30	42.94	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078IS66	3.5e-30	43.21	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
Q6RVV4	1.3e-24	35.76	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
A2RVM0	1.4e-23	35.67	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9HBH5	1.1e-18	38.41	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of Bhi02G000371 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138098.3	6.9e-84	95.91	short-chain dehydrogenase TIC 32 B, chloroplastic [Cucumis sativus] >KGN63560.2 ...	[more]
TYJ98519.1	9.0e-84	95.91	short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa]	[more]
XP_008464537.1	9.0e-84	95.91	PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Cucumis melo]	[more]
KAG7023113.1	9.9e-83	94.15	Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_023516084.1	9.9e-83	94.15	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]

BLAST of Bhi02G000371 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNV3	3.3e-84	95.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G004220 PE=3 SV=1	[more]
A0A5D3BHM5	4.3e-84	95.91	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CLP7	4.3e-84	95.91	short-chain dehydrogenase TIC 32, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7UW68	1.8e-82	94.74	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1JH35	2.4e-82	92.98	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 47..66 score: 24.11 coord: 6..22 score: 33.17 coord: 70..87 score: 32.49
None	No IPR available	GENE3D	3.40.50.720		coord: 1..167 e-value: 3.8E-31 score: 110.2
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 1..166
None	No IPR available	PANTHER	PTHR24320:SF198	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 1..166
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 11..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000371	Bhi02M000371	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity