Bhi02G000287 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000287
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	5-formyltetrahydrofolate cyclo-ligase
Location	chr2: 7140489 .. 7143562 (-)
RNA-Seq Expression	Bhi02G000287
Synteny	Bhi02G000287

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAAAATATCAGCTCCTCCAAGGAAACAATACAAATTTACCGGTACATGGATTTCAAGATTCGATCACTGCTTCTAAGTTGACGAACCAGCGAAGTCCAGCCGGAGTGCACTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTTCGAAGCCCCAATGCTCCGAAACTATAAACCATTATTACCCATTGCAGCCAATTCACTGTTCAATCGCCTCCAACCTTCAGTTTTCCAACGATCCCTTCCTCCATTATCGCGTTCTTCATCCGGGTCCCCAAAAATGGCGAGCAACGAGAGTATCAAACCAGAAAACGAAGACCAATTGAAGGCCACATTTGAACAGAAACGGGTTCTTCGATCCAGCGTTCGAAAAGCCCTCAAAGCCATGGACCCCTCCCTCAGATACCAGGAAGGTAGCAATTGCTTACTTATCTTCATCGATTAACTTCGCCTTTGCTGAAAATGTTTGTCCATGTAATAGCATAGTTAATTGGATATCGTTTTACGATGTTTCTGAATCGACAGAATGCGATCTTCTATTTTCCCTCTTTCATTGGGCTCTATACTTGTTTTTCAGACAATGCAATTCAGAGTATCGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAATGCGTTAAGGGAAGTCGATACATCGAGACTATTATCAGAGACTCTGCAATACCCACCTAAAGGTGTGTTCGTAAACTTATTAGCCGCTTACTCTAATTCATGCGGATGATATATCCGAAGTTTCAAGGATTCTTTTGGCGAGATTAACGCAATTATGTCAGTAAGGCTCAATGAAAGATTTTATATTAGTTTACATCAAATAAAATAAACACAATTAGTTACTAATTCAATTAGATTCTTTAGATCAGTTTAATAATTAGTGTAGGAGAATATAATCATCGTCTGGAAATGGTTTCAATTTCAAACAGAGAGACTATTTGACGCAGTGGATCTGAATGCAGAGTACATGTGGGAACAATTGTTGCTCATGATACGGATAAGTTGTTTTTTGAACATGTTGGTTCTAAGATAATGCTCTGTTTTACTCGGGGAAGTTATTGATTATAGTATGATTCGACTGAGCTTTGTAAATGTTGACATTTTTCTTTTTATCTGAAGATGGTTGTTCAAAGAAGATTTTTGTCCCGCGTGTGGAGGACAAGAATAGTCATATGCGGATGTTCAATATTTCATGTATGGATGATCTTATTGCAAATTCAATGAACATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGGTAACTGAATACTCCACCCCATCTATCTCCTTTGATTTTCTGTTGTCAGTTCCTGGCACAATGGTCGTAGTGATTGTTGTGATCAATTTACAATATATTATATATTGCACTGCCACTGGAATAATGATGTATTTGACTCATGTATTATATATAGCAATTAGTACTGGGATTTGAATAACATTTTAAACCATTTATCTTCATAACATTAAAGATATTATTGTTTTCTCCGGCTTGATCTCCTCTAATGCAATAGTATGGCTTTGCAAATAGAATAGCTCTAGGGTAATTGTGTCCTTATCATCGGGGACCAAAATATTTTTTGTTTACCACCAGCAAAAAGACAGGTTGTTCCTTTTTGTCTTGGATGGTCAATGGATCTATTAATTCTTCAATGCGAGCCAGAACTGAACCTTCCATGGTTTGCATCTTTGACAGAATCCATAAACCAGTATCCCTTAAATACTTGTCCCCCTCCCCCCCCAACAAAAAAAAAAAAAAAACCTGTACCTCTAGTATGTCCCTGGATCTTCTTTGAGCTTGACGATAGGTCATGGAACTGCTGTTTCATTCTTCTTATGTTTTTAGTACCCAATCGTTAACTTATTTTGTTAAACTCCTTAGCCTTGTGAAGTTTTCCTGGAATTTATACGGCATTTTCCCCCAGAGTGGGGAGTGGTTAGCCATGAGTCTACTTTAACTCTTATGTGTTATGGTGGTGATAATTGTTATCAACCAAACATTATTGCCATCGAGAGAACAGAGCATCAGTATTGAATGGTGTTATTGTTCCGTTGCGGGCATAAAACAAATCGATTCCTCTCTCTTCTTTCGATCTGTCAAAACAGTTTGCAGTTTTGATGAGGAAGGATTTGTTACATTCGTTTTCTTTTTTCCTTTTCTAAAAATATATCAACCAAACATTACTGTTATCCATCCATATGATCTCTTTTTTGTTCTTTTTATCTTTTGTGAACAAAAAAATGATTTGACATAATCCATCTCTGCATCTTGAGTGTAACACTGCTGATATTTAAACATCTATGTTTTGCAGTTATGCAGACAAAAGAGCCCATGGATTTATTCCTCTTACCAGGTGAGATATTTGTATCTATTTGTTGGTTGTTTAGGGTTTGTTTATAGGTTAGAAGCCAATGTGAAATCACTTGTTAATATGTTGACTATGATTGCTGCCAGGACTAGCATTTGATAAATCAGGAAGACGCTTAGGCCGAGGGGGAGGGTATGGAATTGCATTTAGCCTTTCTGAGGTTTCTTTCTGCTGCTGTTATTACTTAACTCATACCACATTTGGAGCTGATTTGTGAAGTTGAAACTAGTAGAGTGACTCCCAAATCACGATAGTGAAATCCCCCCTGTGTTTTTTTTTTCTCTGCTTGTTTAGATTTTGCCCTCATTCAATCTGTATTGTCTTTACTTGCTATGAAACCAGTTATTATGATACCTTCCTAAGGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCTCTCCTCGGTATGTGTGCAAGCTCTCTCCTTTCTAGTAGAACCTCACCTTAGTTTCCCTTGCACCTCACCTTGTTTCCCTGCAACTTCACTTGACTCAATTGGAATGATTTGCAGTTGCACTGTCCTATTCGGTGCAGATAATGGATGAAGGAATTATACCACTTACTCCAAACGATGTTTTAATCGATGCTCTTGTATCACCATCTGGTGTGATTCCAATCAGCTCAGTTGGATTGGACAAGTAA

mRNA sequence

ATGATAAAATATCAGCTCCTCCAAGGAAACAATACAAATTTACCGGTACATGGATTTCAAGATTCGATCACTGCTTCTAAGTTGACGAACCAGCGAAGTCCAGCCGGAGTGCACTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTTCGAAGCCCCAATGCTCCGAAACTATAAACCATTATTACCCATTGCAGCCAATTCACTGTTCAATCGCCTCCAACCTTCAGTTTTCCAACGATCCCTTCCTCCATTATCGCGTTCTTCATCCGGGTCCCCAAAAATGGCGAGCAACGAGAGTATCAAACCAGAAAACGAAGACCAATTGAAGGCCACATTTGAACAGAAACGGGTTCTTCGATCCAGCGTTCGAAAAGCCCTCAAAGCCATGGACCCCTCCCTCAGATACCAGGAAGACAATGCAATTCAGAGTATCGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAATGCGTTAAGGGAAGTCGATACATCGAGACTATTATCAGAGACTCTGCAATACCCACCTAAAGATGGTTGTTCAAAGAAGATTTTTGTCCCGCGTGTGGAGGACAAGAATAGTCATATGCGGATGTTCAATATTTCATGTATGGATGATCTTATTGCAAATTCAATGAACATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGAGCCCATGGATTTATTCCTCTTACCAGGACTAGCATTTGATAAATCAGGAAGACGCTTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAGGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCTCTCCTCGTTGCACTGTCCTATTCGGTGCAGATAATGGATGAAGGAATTATACCACTTACTCCAAACGATGTTTTAATCGATGCTCTTGTATCACCATCTGGTGTGATTCCAATCAGCTCAGTTGGATTGGACAAGTAA

Coding sequence (CDS)

ATGATAAAATATCAGCTCCTCCAAGGAAACAATACAAATTTACCGGTACATGGATTTCAAGATTCGATCACTGCTTCTAAGTTGACGAACCAGCGAAGTCCAGCCGGAGTGCACTTGGGTCGGAGCCCAAAACCCAGAAAGTGGGCAATTTTCGAAGCCCCAATGCTCCGAAACTATAAACCATTATTACCCATTGCAGCCAATTCACTGTTCAATCGCCTCCAACCTTCAGTTTTCCAACGATCCCTTCCTCCATTATCGCGTTCTTCATCCGGGTCCCCAAAAATGGCGAGCAACGAGAGTATCAAACCAGAAAACGAAGACCAATTGAAGGCCACATTTGAACAGAAACGGGTTCTTCGATCCAGCGTTCGAAAAGCCCTCAAAGCCATGGACCCCTCCCTCAGATACCAGGAAGACAATGCAATTCAGAGTATCGTCTTGGAAGCTCCGTGGTTTAAATCTAGTCAGAGATTATGTGCTTATGTGAGTTGCAATGCGTTAAGGGAAGTCGATACATCGAGACTATTATCAGAGACTCTGCAATACCCACCTAAAGATGGTTGTTCAAAGAAGATTTTTGTCCCGCGTGTGGAGGACAAGAATAGTCATATGCGGATGTTCAATATTTCATGTATGGATGATCTTATTGCAAATTCAATGAACATCTTAGAACCAGCTCCAGTGGATGGTGATGGAAATGAACGTGAAGATGTTATGCAGACAAAAGAGCCCATGGATTTATTCCTCTTACCAGGACTAGCATTTGATAAATCAGGAAGACGCTTAGGCCGAGGGGGAGGTTATTATGATACCTTCCTAAGGAACTACCAAGAACTTGCAAAGGCACGGAATTGGAAGCAGCCTCTCCTCGTTGCACTGTCCTATTCGGTGCAGATAATGGATGAAGGAATTATACCACTTACTCCAAACGATGTTTTAATCGATGCTCTTGTATCACCATCTGGTGTGATTCCAATCAGCTCAGTTGGATTGGACAAGTAA

Protein sequence

MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYKPLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVLRSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSETLQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQIMDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK

Homology

[Close]

BLAST of Bhi02G000287 vs. TAIR 10
Match: AT5G13050.1 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 318.9 bits (816), Expect = 4.8e-87
Identity = 161/258 (62.40%), Postives = 206/258 (79.84%), Query Frame = 0


Query: 73  RLQPSVFQRSLP--PLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVLRSSVRKALKA 132
           R  P  F   +P  P+ R S  +  + +  S   +N+++L + F+QKRV+RS+VRK+LKA
Sbjct: 15  RRSPIFFFPKIPTRPVFRLSPATRPIVA-MSTTSKNQEELDSIFKQKRVVRSTVRKSLKA 74

Query: 133 MDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSETLQYPPKDGCS 192
           MDPSLR Q+D AIQ  VLEAPWFKS + LCAY+SC +L EVDTS++LSE LQ+P  +   
Sbjct: 75  MDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQ 134

Query: 193 KKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVMQTKEPMDLFL 252
           KK++VP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  EP+DLF+
Sbjct: 135 KKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFI 194

Query: 253 LPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQIMDEGIIPLTP 312
           LPGLAFD+ GRRLGRGGGYYDTFL+ YQ+ AK + W+ PL+VALSYS QI+++G IP+TP
Sbjct: 195 LPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTP 254

Query: 313 NDVLIDALVSPSGVIPIS 329
           NDVLIDALV+PSGV+PI+
Sbjct: 255 NDVLIDALVTPSGVVPIT 270

[Close]

BLAST of Bhi02G000287 vs. TAIR 10
Match: AT5G13050.2 (5-formyltetrahydrofolate cycloligase )

HSP 1 Score: 226.5 bits (576), Expect = 3.2e-59
Identity = 118/196 (60.20%), Postives = 151/196 (77.04%), Query Frame = 0


Query: 73  RLQPSVFQRSLP--PLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVLRSSVRKALKA 132
           R  P  F   +P  P+ R S  +  + +  S   +N+++L + F+QKRV+RS+VRK+LKA
Sbjct: 15  RRSPIFFFPKIPTRPVFRLSPATRPIVA-MSTTSKNQEELDSIFKQKRVVRSTVRKSLKA 74

Query: 133 MDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSETLQYPPKDGCS 192
           MDPSLR Q+D AIQ  VLEAPWFKS + LCAY+SC +L EVDTS++LSE LQ+P  +   
Sbjct: 75  MDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQ 134

Query: 193 KKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVMQTKEPMDLFL 252
           KK++VP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  EP+DLF+
Sbjct: 135 KKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFI 194

Query: 253 LPGLAFDKSGRRLGRG 267
           LPGLAFD+ GRRLGRG
Sbjct: 195 LPGLAFDRCGRRLGRG 208

[Close]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot
Match: Q8L539 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=5FCL PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.7e-86
Identity = 161/258 (62.40%), Postives = 206/258 (79.84%), Query Frame = 0


Query: 73  RLQPSVFQRSLP--PLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVLRSSVRKALKA 132
           R  P  F   +P  P+ R S  +  + +  S   +N+++L + F+QKRV+RS+VRK+LKA
Sbjct: 15  RRSPIFFFPKIPTRPVFRLSPATRPIVA-MSTTSKNQEELDSIFKQKRVVRSTVRKSLKA 74

Query: 133 MDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSETLQYPPKDGCS 192
           MDPSLR Q+D AIQ  VLEAPWFKS + LCAY+SC +L EVDTS++LSE LQ+P  +   
Sbjct: 75  MDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEVDTSKILSEILQHPDSN-TQ 134

Query: 193 KKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVMQTKEPMDLFL 252
           KK++VP VEDKNS+MRM +IS M+DL+ANSMNILEPAPVD  GN+REDV+Q  EP+DLF+
Sbjct: 135 KKLYVPWVEDKNSNMRMLHISHMEDLVANSMNILEPAPVDAQGNDREDVLQADEPIDLFI 194

Query: 253 LPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQIMDEGIIPLTP 312
           LPGLAFD+ GRRLGRGGGYYDTFL+ YQ+ AK + W+ PL+VALSYS QI+++G IP+TP
Sbjct: 195 LPGLAFDRCGRRLGRGGGYYDTFLKRYQDRAKEKGWRYPLMVALSYSPQILEDGSIPVTP 254

Query: 313 NDVLIDALVSPSGVIPIS 329
           NDVLIDALV+PSGV+PI+
Sbjct: 255 NDVLIDALVTPSGVVPIT 270

[Close]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot
Match: P49914 (5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 4.6e-18
Identity = 64/214 (29.91%), Postives = 111/214 (51.87%), Query Frame = 0


Query: 112 ATFEQKRVLRSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREV 171
           A    KR LR  +++ L+AM    R ++   +   V+    ++ S+R+  ++S     E+
Sbjct: 5   AVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQ--DEI 64

Query: 172 DTSRLLSETLQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDD---LIANSMNILEPAP 231
           +T  ++ +  Q        K  F+PR   +++HM M  I   ++   L   S NI +P  
Sbjct: 65  ETEEIIKDIFQR------GKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQP-- 124

Query: 232 VDGDGNEREDVMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLR---NYQELAKARN 291
             G+G+ RE+ + T   +DL  +PGL FDK G RLGRG GYYD +L+    +QE+     
Sbjct: 125 --GEGDVREEALST-GGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEV----- 184

Query: 292 WKQPLLVALSYSVQIMDEGIIPLTPNDVLIDALV 320
             +P  +AL++  QI  +  +P+  ND+ +D ++
Sbjct: 185 --KPYTLALAFKEQICLQ--VPVNENDMKVDEVL 196

[Close]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot
Match: Q9D110 (5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 1.7e-17
Identity = 63/209 (30.14%), Postives = 115/209 (55.02%), Query Frame = 0


Query: 117 KRVLRSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRL 176
           KR LR+ +++ L+A+    R ++   +   V+    +++S+R+  ++S     EV+T  +
Sbjct: 10  KRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHNQYQNSKRISIFLSMQ--DEVETEVI 69

Query: 177 LSETLQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDD---LIANSMNILEPAPVDGDG 236
           + +  +        K  F+PR + +++HM M  ++  ++   L   S NI +P    G+G
Sbjct: 70  IKDIFKQ------GKICFIPRYQFQSNHMDMVRLTSSEEIALLPKTSWNIHQP----GEG 129

Query: 237 NEREDVMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLR---NYQELAKARNWKQPL 296
           + RE+ + T   +DL  LPGL FDK G RLGRG GYYDT+L+    +QE+       +P 
Sbjct: 130 DVREEALST-GGLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRCVQHQEV-------KPY 189

Query: 297 LVALSYSVQIMDEGIIPLTPNDVLIDALV 320
            +AL++  QI  +  IP+  +D+ +D ++
Sbjct: 190 TMALAFKEQICPQ--IPVDEHDMKVDEVL 196

[Close]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot
Match: P80405 (5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.1e-17
Identity = 59/206 (28.64%), Postives = 110/206 (53.40%), Query Frame = 0


Query: 117 KRVLRSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRL 176
           KR LR+ +++ L+A+    R +    +   V+    ++ SQR+  ++S     E++T  +
Sbjct: 10  KRSLRAELKQRLRAISAEERLRCQRLLTQKVIAHRQYQKSQRISIFLSMP--DEIETEEI 69

Query: 177 LSETLQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIA---NSMNILEPAPVDGDG 236
           + +  Q        K  F+PR   +++HM M  ++  D++ +    S NI +P+    + 
Sbjct: 70  IKDIFQQ------GKVCFIPRYRLQSNHMDMVKLASADEISSLPKTSWNIHQPS----ES 129

Query: 237 NEREDVMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVA 296
           + RE+ + T   +DL  +PGL FD++G RLGRG GYYDT+L+   +   A+    P  +A
Sbjct: 130 DTREEALAT-GGLDLIFMPGLGFDRNGNRLGRGRGYYDTYLQRCLQQQGAK----PYTIA 189

Query: 297 LSYSVQIMDEGIIPLTPNDVLIDALV 320
           L++  QI  +  +P+   DV +D ++
Sbjct: 190 LAFREQICPQ--VPVDDTDVSVDEVL 196

[Close]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot
Match: Q9XWE6 (Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239 GN=Y106G6E.4 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-13
Identity = 57/213 (26.76%), Postives = 98/213 (46.01%), Query Frame = 0


Query: 110 LKATFEQKRVLRSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALR 169
           + A  E K  LR  ++  L  +      ++  A+   ++E+ WF+ S+RL  YVS +   
Sbjct: 1   MSAIKEVKSELRQFMKTLLGKISKEETQRQTEAVFEKIIESKWFQESKRLSVYVSTSG-- 60

Query: 170 EVDTSRLLSETLQYPPKDGCSKKIFVPRVEDKNSHMRMFNI---SCMDDLIANSMNILEP 229
           E+ T  ++ + L+        K++F+P+    ++ M M  +   +  D+L +    I +P
Sbjct: 61  EIQTDSIIQKALE------MGKEVFIPQFTKGSTAMDMVRVPDQTAFDNLPSTLWGIRQP 120

Query: 230 APVDGDGNEREDVMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNW 289
            P       +        P+DL L PG+AF   G R G G GYYD F   +    K    
Sbjct: 121 EP-----KWKWQSYHETGPLDLILAPGVAFSPYGLRCGHGKGYYDRFFSTHH---KHFPE 180

Query: 290 KQPLLVALSYSVQIMDEGIIPLTPNDVLIDALV 320
             P  + L+   QI+  G IP++  DV +D ++
Sbjct: 181 NSPKKIGLALREQII--GTIPISETDVELDEVI 195

[Close]

BLAST of Bhi02G000287 vs. NCBI nr
Match: XP_038879322.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Benincasa hispida])

HSP 1 Score: 664.5 bits (1713), Expect = 4.9e-187
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK
Sbjct: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL
Sbjct: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET
Sbjct: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK
Sbjct: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 334

[Close]

BLAST of Bhi02G000287 vs. NCBI nr
Match: XP_004138292.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 298/334 (89.22%), Postives = 314/334 (94.01%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S T  +LTNQ SPAGVHLGRSPKP+KWAI EAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENEDQLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPS R QEDN IQSIVLEAPWFKSSQRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+DALVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK 334

[Close]

BLAST of Bhi02G000287 vs. NCBI nr
Match: KGN63636.2 (hypothetical protein Csa_013278 [Cucumis sativus])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 298/334 (89.22%), Postives = 314/334 (94.01%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S T  +LTNQ SPAGVHLGRSPKP+KWAI EAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENEDQLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPS R QEDN IQSIVLEAPWFKSSQRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+DALVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK 334

[Close]

BLAST of Bhi02G000287 vs. NCBI nr
Match: XP_008453155.1 (PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 582.0 bits (1499), Expect = 3.2e-162
Identity = 293/333 (87.99%), Postives = 310/333 (93.09%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S   S+LTNQ SPAGVHLGRSPK RKWAI EAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTIGSRLTNQLSPAGVHLGRSPKHRKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENEDQLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGCPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDN IQSIVLEAPWFKS+QRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSTQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVD DGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDSDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKA+NWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKAQNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLD 334
           MDEGIIPLTP DVL+DALVSPSGVIPISS  LD
Sbjct: 301 MDEGIIPLTPKDVLVDALVSPSGVIPISSAALD 333

[Close]

BLAST of Bhi02G000287 vs. NCBI nr
Match: XP_022988398.1 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 574.7 bits (1480), Expect = 5.2e-160
Identity = 292/334 (87.43%), Postives = 311/334 (93.11%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           MIK QLLQGN TNLPVHG Q+S TAS+LTN RSPAGVH+GRSPK R+ AI E+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
            LLPIAA+SLFNRL PS FQ +L PLSR SSGSPKMA NE IKPENE+QLKA FEQKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDNAIQ+IVLEAPWFKSSQRLCAYVSC+ALREVDTSR+LSET
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+D LVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDK 333

[Close]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL
Match: A0A0A0LRK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.2e-166
Identity = 298/334 (89.22%), Postives = 314/334 (94.01%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S T  +LTNQ SPAGVHLGRSPKP+KWAI EAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTTGFRLTNQLSPAGVHLGRSPKPQKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENEDQLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGYPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPS R QEDN IQSIVLEAPWFKSSQRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSFRSQEDNVIQSIVLEAPWFKSSQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGHSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKARNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKARNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+DALVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDALVSPSGVIPISSAGLDK 334

[Close]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL
Match: A0A1S3BVI2 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493953 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.6e-162
Identity = 293/333 (87.99%), Postives = 310/333 (93.09%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           M+KYQLLQGN TNLPV+GFQ+S   S+LTNQ SPAGVHLGRSPK RKWAI EAPMLRNYK
Sbjct: 1   MVKYQLLQGNRTNLPVNGFQNSTIGSRLTNQLSPAGVHLGRSPKHRKWAISEAPMLRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
           PLLPIAANSLFN +Q S FQ+SLPPLSR SSG PKMA+NESIKPENEDQLK  F+QKRVL
Sbjct: 61  PLLPIAANSLFNHIQSSAFQQSLPPLSRFSSGCPKMATNESIKPENEDQLKHIFDQKRVL 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDN IQSIVLEAPWFKS+QRLCAYVSC+ALREVDTSRLLSE 
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNVIQSIVLEAPWFKSTQRLCAYVSCSALREVDTSRLLSEI 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVD DGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDSDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAKA+NWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKAQNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLD 334
           MDEGIIPLTP DVL+DALVSPSGVIPISS  LD
Sbjct: 301 MDEGIIPLTPKDVLVDALVSPSGVIPISSAALD 333

[Close]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL
Match: A0A6J1JM66 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.5e-160
Identity = 292/334 (87.43%), Postives = 311/334 (93.11%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           MIK QLLQGN TNLPVHG Q+S TAS+LTN RSPAGVH+GRSPK R+ AI E+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
            LLPIAA+SLFNRL PS FQ +L PLSR SSGSPKMA NE IKPENE+QLKA FEQKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDNAIQ+IVLEAPWFKSSQRLCAYVSC+ALREVDTSR+LSET
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
           QTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 QTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+D LVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDK 333

[Close]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL
Match: A0A6J1JCX7 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485651 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.1e-158
Identity = 291/336 (86.61%), Postives = 310/336 (92.26%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           MIK QLLQGN TNLPVHG Q+S TAS+LTN RSPAGVH+GRSPK R+ AI E+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRSPAGVHVGRSPKARRRAISESPMVRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
            LLPIAA+SLFNRL PS FQ +L PLSR SSGSPKMA NE IKPENE+QLKA FEQKRV+
Sbjct: 61  -LLPIAASSLFNRLPPSAFQPALRPLSRCSSGSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDNAIQ+IVLEAPWFKSSQRLCAYVSC+ALREVDTSR+LSET
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNERED-- 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNERED  
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDGN 240

Query: 241 VMQTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSV 300
            MQTK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAK RNWKQPLLVALSYSV
Sbjct: 241 FMQTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSV 300

Query: 301 QIMDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           QIMDEGIIPLTPNDVL+D LVSPSGVIPISS GLDK
Sbjct: 301 QIMDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDK 335

[Close]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL
Match: A0A6J1E0S0 (5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429770 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.8e-157
Identity = 286/334 (85.63%), Postives = 308/334 (92.22%), Query Frame = 0


Query: 1   MIKYQLLQGNNTNLPVHGFQDSITASKLTNQRSPAGVHLGRSPKPRKWAIFEAPMLRNYK 60
           MIK QLLQGN TNLPVHG Q+S TAS+LTN R+PAGVH+GRSPK R+ A+ E+PM+RNYK
Sbjct: 1   MIKSQLLQGNKTNLPVHGLQNSTTASRLTNPRNPAGVHVGRSPKARRRAVSESPMVRNYK 60

Query: 61  PLLPIAANSLFNRLQPSVFQRSLPPLSRSSSGSPKMASNESIKPENEDQLKATFEQKRVL 120
            LLPIAA+S FNRL P+ FQ +L PLSR SS SPKMA NE IKPENE+QLKA FEQKRV+
Sbjct: 61  -LLPIAASSPFNRLPPTAFQPALRPLSRCSSRSPKMARNEGIKPENEEQLKALFEQKRVI 120

Query: 121 RSSVRKALKAMDPSLRYQEDNAIQSIVLEAPWFKSSQRLCAYVSCNALREVDTSRLLSET 180
           RSSVRKALKAMDPSLR QEDNAIQ+IVLEAPWFKSSQRLCAYVSC+ALREVDTSR+LSET
Sbjct: 121 RSSVRKALKAMDPSLRSQEDNAIQNIVLEAPWFKSSQRLCAYVSCSALREVDTSRILSET 180

Query: 181 LQYPPKDGCSKKIFVPRVEDKNSHMRMFNISCMDDLIANSMNILEPAPVDGDGNEREDVM 240
           LQ+PPKDG SKKI+VPRVEDKNSHMRMFNIS MDDLIANSMNILEPAPVDGDGNEREDVM
Sbjct: 181 LQHPPKDGYSKKIYVPRVEDKNSHMRMFNISRMDDLIANSMNILEPAPVDGDGNEREDVM 240

Query: 241 QTKEPMDLFLLPGLAFDKSGRRLGRGGGYYDTFLRNYQELAKARNWKQPLLVALSYSVQI 300
            TK+P+DLFLLPGLAFDKSGRRLGRGGGYYDTFL+NYQELAK RNWKQPLLVALSYSVQI
Sbjct: 241 LTKDPIDLFLLPGLAFDKSGRRLGRGGGYYDTFLKNYQELAKVRNWKQPLLVALSYSVQI 300

Query: 301 MDEGIIPLTPNDVLIDALVSPSGVIPISSVGLDK 335
           MDEGIIPLTPNDVL+D LVSPSGVIPISS GLDK
Sbjct: 301 MDEGIIPLTPNDVLVDVLVSPSGVIPISSAGLDK 333

The following BLAST results are available for this feature:

BLAST of Bhi02G000287 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13050.1	4.8e-87	62.40	5-formyltetrahydrofolate cycloligase	[more]
AT5G13050.2	3.2e-59	60.20	5-formyltetrahydrofolate cycloligase	[more]

BLAST of Bhi02G000287 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L539	6.7e-86	62.40	5-formyltetrahydrofolate cyclo-ligase, mitochondrial OS=Arabidopsis thaliana OX=...	[more]
P49914	4.6e-18	29.91	5-formyltetrahydrofolate cyclo-ligase OS=Homo sapiens OX=9606 GN=MTHFS PE=1 SV=2	[more]
Q9D110	1.7e-17	30.14	5-formyltetrahydrofolate cyclo-ligase OS=Mus musculus OX=10090 GN=Mthfs PE=2 SV=...	[more]
P80405	5.1e-17	28.64	5-formyltetrahydrofolate cyclo-ligase OS=Oryctolagus cuniculus OX=9986 GN=MTHFS ...	[more]
Q9XWE6	2.6e-13	26.76	Probable 5-formyltetrahydrofolate cyclo-ligase OS=Caenorhabditis elegans OX=6239...	[more]

BLAST of Bhi02G000287 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879322.1	4.9e-187	100.00	5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Benincasa hispida]	[more]
XP_004138292.1	1.1e-165	89.22	5-formyltetrahydrofolate cyclo-ligase, mitochondrial [Cucumis sativus]	[more]
KGN63636.2	1.1e-165	89.22	hypothetical protein Csa_013278 [Cucumis sativus]	[more]
XP_008453155.1	3.2e-162	87.99	PREDICTED: 5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 [Cucu...	[more]
XP_022988398.1	5.2e-160	87.43	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 [Cucurbita ...	[more]

BLAST of Bhi02G000287 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRK4	5.2e-166	89.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G007880 PE=4 SV=1	[more]
A0A1S3BVI2	1.6e-162	87.99	5-formyltetrahydrofolate cyclo-ligase, mitochondrial isoform X1 OS=Cucumis melo ...	[more]
A0A6J1JM66	2.5e-160	87.43	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit...	[more]
A0A6J1JCX7	3.1e-158	86.61	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X1 OS=Cucurbit...	[more]
A0A6J1E0S0	5.8e-157	85.63	5-formyltetrahydrofolate cyclo-ligase, mitochondrial-like isoform X2 OS=Cucurbit...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002698	5-formyltetrahydrofolate cyclo-ligase	TIGRFAM	TIGR02727	TIGR02727	coord: 117..320 e-value: 1.5E-28 score: 97.9
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PFAM	PF01812	5-FTHF_cyc-lig	coord: 117..321 e-value: 8.7E-31 score: 107.2
IPR024185	5-formyltetrahydrofolate cyclo-ligase-like domain superfamily	GENE3D	3.40.50.10420		coord: 109..324 e-value: 6.3E-53 score: 181.3
None	No IPR available	PIRSR	PIRSR006806-1	PIRSR006806-1	coord: 116..230 e-value: 2.6E-10 score: 38.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..106
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..100
None	No IPR available	PANTHER	PTHR23407	ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE	coord: 89..333
None	No IPR available	PANTHER	PTHR23407:SF10	5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE, MITOCHONDRIAL-LIKE ISOFORM X1	coord: 89..333
IPR037171	NagB/RpiA transferase-like	SUPERFAMILY	100950	NagB/RpiA/CoA transferase-like	coord: 116..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000287	Bhi02M000287	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0031213	RSF complex
molecular_function	GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding