Bhi02G000208 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000208
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionExonuclease domain-containing protein
Locationchr2: 5649564 .. 5653869 (-)
RNA-Seq ExpressionBhi02G000208
SyntenyBhi02G000208
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAATTTACGGGAGTTAATGATAATTCAAATTTTTTTTTTAATATAGTAGGATCAAACCTTTAATTTTTTTTTTTTTAAATGTCATGTCAATTATTGTTGAACAAATTTTAACAACTTAAAAGTAAAATTGGGCTTAATAAAAAAATATGAGCTTTAAATCTCAAAAAAAAAAGAAAAAAGAAAAAAAAGAGAAGAAAGTTTGCATAACCAAGTCTTGGATGATTTAAAAAGTTGAAAATGAGCATAATATGAATCTGGCCGATTATTCAATATAAACAACAACAACAATAATAATACTGATCATCATCGTCGTAAATTATTTTAGAGAGATTTTAAAAAATAGAAAATTTTAAAATATGGATTATTTAATTTAGAAATTGGAATGAGAATGAAGGGGATGATCCGTTGATTGGAAGTTCAGTGCAGGAAGCAAGCTCTTAAAGCCAGGGCGGTCGTTAACGTTATAATAATATCTTCACCTCTTCCCGCCTTTCTTCTTGTTCTTCTTGTTCTTCTTCTTCAATCTCGTATAAATCTACTAATCTTCTTTCTTTTCCCTCTGTTCTCTTCTTTGATTGAAAATTCCATTATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGTGCTCACTCCTCTCTCTCTCTCTCTTTTGATTCCACACTAATTCTCTACTAGGGTATTTGAAAGGAAGCGGCGAAATGTTATCTATACATGTCCTTCTTTTTGATGTATTATGAATGTGGATTGATTGTTAAATTTATGTGGAAGTTGAATTGGGTGCCTTCTGATGGGACTGGGAGCTGGTTTCTGTTAAACAAGAGATTTTGGCCCAGAATTGTGTTCTTTTTTCTTCTGAACTTCAGACGACATTTCCTTAGTCAGGTTAGTTTAGTTGAAGGATGGTAACATTTTTAGGGACCCAAGTTTGTGTAAGGTTCGAAGAGACGAACATTGGTCGGGAGGCTTCTCAGACACTCTTCTTTGACTTCGCCCTTTTTTTCCCTACCAAATCTTTTTAGCAAGTGTGTATAGGTTTAAATTCAGATTACATTTCTCGGTTAGATGATCATTTCAGTTCTAAGATTGTACCATGCACTGTTGGGTTGAATGTTCCTGCTTCTTCTAGTTTAATTCTATTATATGCCTGTGTTTGAGTTTCCTCTGCTGCGATACTGTTTCCTGTAGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGGTAATGACAGAATTCTCTCTCTGTTGCATTCTTGCTATGTTTCTGCTTGTGTTCATGCAAAATTTCAGGGCTAGTCTCCATTAGACGGTACTTTTGTCAAATTTGTTTAGAGCTTTCAACATTCAGAACTCAAGCAGGAAATTGTAGAAGTAATTGAAGTTTTGGGATTTACCTCTCTAATTGAGACTTAAACCATGTTAACATTTGGCACTATGATAGGATTATATTACAAATGTAACATTTTACCAATTTAATATATCATGGTTAAATCTTATGCGAACAAGGGATGGAAAAACCTCTTTTTGGGGAAGATCTGTAATGATGAGGGATTAAGATTTGAAAAATTATGCATAAAGTGGTGAGAATAGGGTTCTTGTAATGATTTTTCTCTTCCTTGCGCTGAGTTTCTCTGGTAAATTCAACCCCATTGTCTTGGCTTTATATAGTTATCTTCATGCATATTCTGACTTTGGGTTTTGGTTTCTAGAAGTTATTGTAACTCTTTATTTATTTTATTTTATTTTTGTTTTTGACGTTCCTTTTCTCCCTGTAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGCAACCCTTCCAACTAGCTAGTAATTTTTTTAACTTAAATTAAAGGAGTGGTTCTAGTTATCTGACTTTGTCTGTGTAATTCTCCAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGTAATGTATAAATTTAAGATGTCGAACATAACTAATATGTTCATGTAAACTTGTAACTTTGTCTGTCTACAATTCATTAATTGTTTGGAGTTTAGACCATTGCCGATGATTACATGTATGGAATTTTTGTTGCATTATCTGAATATTGACCACTTGGATGTATGCCCGTGATAACCACAATTCTTTCTGAATTTAGATCATCAATAGGCTCCAAATGCTTCATTGTCAGTTTTTGCATCAATTGACAATACTTGTCTCCATTTTGCCTATCTGCCATGTGGAGACATTCTTCTTGTACTTTTTCTCTCTCTTTCTTTTTGAAGTAAAGTTTATTTGACAACAGCATAGAATTAGTTTTGCTCTTATAATGACCAGTTTATATATAATATAGTATGGTGGGTTAAGTTGAACTATTGTCTGGCTATAGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACAGTAAGTCATACTCCCATCTGATAGTTTGACATATTAGTTAACTACTATCTACAAATATTTTGATGAATTGAACTATCAAATGTGGGAAAAGGAAGAGAAAATAACAGAGAACATCTAGTCCAACGTATTATCCGCAAGCTTGCTTCTTTGCTTCAATCTTTGGTTTCTTGCCATTCATATCGATTCATCTGCTCTGTAAGTTCTTAATTTCCACTTCTTGGTCAGTTTCTAAAGGATATTAAATTTGTTTTTTCACAGCATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAGTAATGGGTGATCAAGTTGTCCCATTATTGAAGTAGCAGTGAATACTGATGTTATGTTATATTATAGTTAGCTTGCTGACTAACATTCAAATTTCCAATTAGAATAATACTAAATTGATACAAGCTCCCGGTTCTTTGAAAGATCATTCAGTTATTTGTGTTTCTAATCAACTGTCTATACTACTTATGAACACATTAGACTTGATACTTTTCTAGAGACATTTCTGACACTTATAGGAGTGAAAAACATTTGAAATTTGTGGACAAACTATATTATTTTGGTTCTTGAAATAATATGTTTAGCAG

mRNA sequence

AATAAATTTACGGGAGTTAATGATAATTCAAATTTTTTTTTTAATATAGTAGGATCAAACCTTTAATTTTTTTTTTTTTAAATGTCATGTCAATTATTGTTGAACAAATTTTAACAACTTAAAAGTAAAATTGGGCTTAATAAAAAAATATGAGCTTTAAATCTCAAAAAAAAAAGAAAAAAGAAAAAAAAGAGAAGAAAGTTTGCATAACCAAGTCTTGGATGATTTAAAAAGTTGAAAATGAGCATAATATGAATCTGGCCGATTATTCAATATAAACAACAACAACAATAATAATACTGATCATCATCGTCGTAAATTATTTTAGAGAGATTTTAAAAAATAGAAAATTTTAAAATATGGATTATTTAATTTAGAAATTGGAATGAGAATGAAGGGGATGATCCGTTGATTGGAAGTTCAGTGCAGGAAGCAAGCTCTTAAAGCCAGGGCGGTCGTTAACGTTATAATAATATCTTCACCTCTTCCCGCCTTTCTTCTTGTTCTTCTTGTTCTTCTTCTTCAATCTCGTATAAATCTACTAATCTTCTTTCTTTTCCCTCTGTTCTCTTCTTTGATTGAAAATTCCATTATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACACATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAGTAATGGGTGATCAAGTTGTCCCATTATTGAAGTAGCAGTGAATACTGATGTTATGTTATATTATAGTTAGCTTGCTGACTAACATTCAAATTTCCAATTAGAATAATACTAAATTGATACAAGCTCCCGGTTCTTTGAAAGATCATTCAGTTATTTGTGTTTCTAATCAACTGTCTATACTACTTATGAACACATTAGACTTGATACTTTTCTAGAGACATTTCTGACACTTATAGGAGTGAAAAACATTTGAAATTTGTGGACAAACTATATTATTTTGGTTCTTGAAATAATATGTTTAGCAG

Coding sequence (CDS)

ATGGGTTCAACGGAGGATCGATCGGAGATTGCCTTCTTTGACGTCGAGACCACCGTTCCCACCCGCCAAGGCCAGAGATTTTCAATTTTGGAATTTGGAGCCATTCTGGTGTGCCCTAGGAAGCTGGTTGAGTTGGAGAGCTATTCCACTCTCGTTCGACCTTCTGATCTCTCGCTCATTTCCTCCTTATCGGTGCGATGCAATGGCATTACTAGAGATGCCGTCATTTCTTCACCTACTTTCGCCCAAATTGCTGATAGGGTTTACGATATCCTTCACGGGCGGATATGGGCAGGCCATAACATACTCAGATTTGACTGTGCACGAATAAGGGAGGCTTTTGCAGAAATTGGGGTCCCAGCACCGGAGCCAAAAGGTACAATTGATTCATTGGCATTGTTGACTCAAAGGTTTGGAAGGAGAGCTGGCGACATGAAGATGGCTACTCTTGCTTCCTATTTTGGAATAGGACAACAAACACACAGGAGTTTGGATGATGTCAGGATGAATCTTGAAGTTCTAAAGTATTGTGCAACTGTCTTGTTTCTGGAATCAAGTCTACCCGAAGTTTTCCGAGAGAACAGTTGGGTTTCTCCAAATGCTGTTACTAGGCGCCGCACTGCAAAATCATCTCCACAGGGGAATAGTTCGAATAATGATACTCACTCATCAAGTTCAAGGATTGGTGGTAATCCCATATCTCTATTAGATCAAGGGGAAACTCATCCGATACTATCCCTCGTAACTTGCAGCTCTGAGGATGGAGCCTCAAATCTGGCTGAATCTAGAGCAACTGAATCAGATTCTTTTAACATGCGCACTCTCAGCGACCAAATAACTGGTGAATCGCTCGAAACAGATATCAGCATGGAAGAAGAACATGCCACAGTATCTACTGAAGCATCTCCCTCAGAGGATACTTCCACAAGTCTCAGCGCTACTACTGAATTTTTGGAGCCTGATCAGGTGTCTGTTTCATTCATCACTGCATCTTTCGTTCCATTTTTTCGTGGGAGCCAAAGAATACAATTATCGCACAAAGATGACTGTTTGCAGCTTCTTTGTAATAATCTGAGAGTTCGGTTTGGCATAAGCACAAAATTCACTGATTACGCTGGCCGTCCAAAGTTGAGTTTCGTGGTTGATGTACCACCAAGTTTATGTACGGTTCTTGAAGCATCTGATGGCGTGGCTCAGAGATTATTCTCTGATTCCGGGAGCGGGTCTGAATGGAGGCCTGTGGTGATGAGAAAGAATGATTATTTCAACTACCCGACAATGAGATTACACATTCCAACTGCAGTAAGTGGAGATGTGGCGATTTATGCTACAGAGATGTACCAAAAAGAATCATCAGGCGCTGCACAAAGGCTGATATTCAGTAAATTCGATGCTGCAGAGCTTGATAGCTTGATCAAACCTGGAGCCATCTTAGATGCATTCATTTCCTTGGATACATATGACTATCAACAGAGTGCAGGCATAAGATTGGTAGCTAAAAGGTTGATCATCCATTCCTAG

Protein sequence

MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS
Homology
BLAST of Bhi02G000208 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-149
Identity = 293/505 (58.02%), Postives = 361/505 (71.49%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPI 244
           SSLP    +NS VSP  ++ RR   +S +GN     T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN-----TVTTSVRL---------------- 243

Query: 245 LSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGES-LETDISMEEEHATVSTEASP 304
                        +++E+ A + D FNM  L +++  ++ +++DI MEEE    S +   
Sbjct: 244 ------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPS-DVVA 303

Query: 305 SEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLRVR 364
           SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++R
Sbjct: 304 SENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIR 363

Query: 365 FGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDY 424
           FG++ KF D  GR +L+FVVD+ PSL ++LEA D  AQ+L  DSGS SEW PVV     +
Sbjct: 364 FGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGF 423

Query: 425 FNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAF 484
            NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G+++DAF
Sbjct: 424 VNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAF 468

Query: 485 ISLDTYDYQQSAGIRLVAKRLIIHS 507
           +SL+ YDYQQ AGIRLVAK+L+I S
Sbjct: 484 LSLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Bhi02G000208 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 515.0 bits (1325), Expect = 6.8e-146
Identity = 288/508 (56.69%), Postives = 357/508 (70.28%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  S
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           V+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PEP
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 185 SSLPEVFRENSWVSPNAVT---RRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGET 244
           SSLP+   ENS  +    T   RRRT K SP  + ++  T  + + I             
Sbjct: 191 SSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI------------- 250

Query: 245 HPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEA 304
            PILS V+            S   ++D F+M TL ++I  E L++D+ MEEE     +E 
Sbjct: 251 -PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQSET 310

Query: 305 SPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLR 364
             SE T         F+E D++SVS I A+ VP + GSQ  ++QL   D  LQL C  L+
Sbjct: 311 VASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRLK 370

Query: 365 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 424
           VRFGI+ KF D AGR +L+FV+D+ PSLC VL+  D  AQ +  DSGSGS+W P+V+   
Sbjct: 371 VRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPMK 430

Query: 425 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSG--AAQRLIFSKFDAAELDSLIKPGAI 484
            + N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+ P  +
Sbjct: 431 GFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRTV 487

Query: 485 LDAFISLDTYDYQQSAGIRLVAKRLIIH 506
           LDAF+SL+ YDYQQ AGIRLVA++L+IH
Sbjct: 491 LDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Bhi02G000208 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.2e-143
Identity = 273/510 (53.53%), Postives = 357/510 (70.00%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFRENSWVSPNAVTRRRT--------AKSSPQGNSSNNDTHSSSSRIGGNPISLL 244
           SS+P++  + SW SP    R R+         + S   +SS+  T  SSS +    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIV--- 247

Query: 245 DQGETHPILSLVT-CSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 304
              + HPI+SL+T CS  D +S   E      D  ++ TL  ++   +L+TD + E +  
Sbjct: 248 ---KNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAKTV 307

Query: 305 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 364
               E+ PS D+    +    FL  ++VSVS I AS VPF+RGS R++L H D  L L  
Sbjct: 308 RQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 424
           ++L+VRFGIS KF D+AGRPKL+ +VD P  LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESS-GAAQRLIFSKFDAAELDSLIKP 484
           +RK  + NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 GAILDAFISLDTYDYQQSAGIRLVAKRLII 505
           G ++DAF SL+ Y YQQ AGIRL  K+L+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Bhi02G000208 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 2.1e-134
Identity = 263/510 (51.57%), Postives = 346/510 (67.84%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLE           E
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE-----------E 187

Query: 185 SSLPEVFRENSWVSPNAVTRRRT--------AKSSPQGNSSNNDTHSSSSRIGGNPISLL 244
           SS+P++  + SW SP    R R+         + S   +SS+  T  SSS +    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIV--- 247

Query: 245 DQGETHPILSLVT-CSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 304
              + HPI+SL+T CS  D +S   E      D  ++ TL  ++   +L+TD + E +  
Sbjct: 248 ---KNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAKTV 307

Query: 305 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 364
               E+ PS D+    +    FL  ++VSVS I AS VPF+RGS R++L H D  L L  
Sbjct: 308 RQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 424
           ++L+VRFGIS KF D+AGRPKL+ +VD P  LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESS-GAAQRLIFSKFDAAELDSLIKP 484
           +RK  + NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 GAILDAFISLDTYDYQQSAGIRLVAKRLII 505
           G ++DAF SL+ Y YQQ AGIRL  K+L+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 491

BLAST of Bhi02G000208 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 3.6e-70
Identity = 125/199 (62.81%), Postives = 158/199 (79.40%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGT 127
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFRENSWVSPNAVTRRR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

BLAST of Bhi02G000208 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.4e-148
Identity = 293/505 (58.02%), Postives = 361/505 (71.49%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETTVP R GQRF+ILEFG+ILVCP+KL EL SY+TLV+P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   PEP
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGETHPI 244
           SSLP    +NS VSP  ++ RR   +S +GN     T ++S R+                
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN-----TVTTSVRL---------------- 243

Query: 245 LSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGES-LETDISMEEEHATVSTEASP 304
                        +++E+ A + D FNM  L +++  ++ +++DI MEEE    S +   
Sbjct: 244 ------------PSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPS-DVVA 303

Query: 305 SEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLRVR 364
           SE+TS        FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++R
Sbjct: 304 SENTSDH----EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIR 363

Query: 365 FGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKNDY 424
           FG++ KF D  GR +L+FVVD+ PSL ++LEA D  AQ+L  DSGS SEW PVV     +
Sbjct: 364 FGVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGF 423

Query: 425 FNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDAF 484
            NYP  R+HI T ++GD A YATE++Q+ESSGA Q+LIFS  +  EL+SL+  G+++DAF
Sbjct: 424 VNYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAF 468

Query: 485 ISLDTYDYQQSAGIRLVAKRLIIHS 507
           +SL+ YDYQQ AGIRLVAK+L+I S
Sbjct: 484 LSLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Bhi02G000208 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.6e-145
Identity = 288/508 (56.69%), Postives = 357/508 (70.28%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           EDRSEIAFFDVETT+P R GQ ++ILEFG+ILVCP+KLVEL++YS LVRP++L+LI+  S
Sbjct: 11  EDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRS 70

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
           V+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   PEP
Sbjct: 71  VKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEP 130

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLFLE
Sbjct: 131 KGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLE 190

Query: 185 SSLPEVFRENSWVSPNAVT---RRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGET 244
           SSLP+   ENS  +    T   RRRT K SP  + ++  T  + + I             
Sbjct: 191 SSLPDELIENSVTTTTPETSSRRRRTIKKSPLQSPTDQQTGENMTTI------------- 250

Query: 245 HPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTEA 304
            PILS V+            S   ++D F+M TL ++I  E L++D+ MEEE     +E 
Sbjct: 251 -PILSFVS------------SAEAQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ-NQQSET 310

Query: 305 SPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQ--RIQLSHKDDCLQLLCNNLR 364
             SE T         F+E D++SVS I A+ VP + GSQ  ++QL   D  LQL C  L+
Sbjct: 311 VASEGTGDQ----EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLHCPRLK 370

Query: 365 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 424
           VRFGI+ KF D AGR +L+FV+D+ PSLC VL+  D  AQ +  DSGSGS+W P+V+   
Sbjct: 371 VRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPLVIPMK 430

Query: 425 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSG--AAQRLIFSKFDAAELDSLIKPGAI 484
            + N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+ P  +
Sbjct: 431 GFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLLNPRTV 487

Query: 485 LDAFISLDTYDYQQSAGIRLVAKRLIIH 506
           LDAF+SL+ YDYQQ AGIRLVA++L+IH
Sbjct: 491 LDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Bhi02G000208 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.8e-142
Identity = 273/510 (53.53%), Postives = 357/510 (70.00%), Query Frame = 0

Query: 5   EDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLS 64
           ++RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCPR+L EL SYSTLVRP+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEP 124
            R +GITRD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  PEP
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFRENSWVSPNAVTRRRT--------AKSSPQGNSSNNDTHSSSSRIGGNPISLL 244
           SS+P++  + SW SP    R R+         + S   +SS+  T  SSS +    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIV--- 247

Query: 245 DQGETHPILSLVT-CSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHA 304
              + HPI+SL+T CS  D +S   E      D  ++ TL  ++   +L+TD + E +  
Sbjct: 248 ---KNHPIVSLLTECSESDASSYDIE------DPIDITTLIGKLRIGTLQTDAAEEAKTV 307

Query: 305 TVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLC 364
               E+ PS D+    +    FL  ++VSVS I AS VPF+RGS R++L H D  L L  
Sbjct: 308 RQQDESPPSPDSD---AKDESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVV 424
           ++L+VRFGIS KF D+AGRPKL+ +VD P  LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 MRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESS-GAAQRLIFSKFDAAELDSLIKP 484
           +RK  + NYPT RLHI +  +GD  +  T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 GAILDAFISLDTYDYQQSAGIRLVAKRLII 505
           G ++DAF SL+ Y YQQ AGIRL  K+L+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Bhi02G000208 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.1e-69
Identity = 125/199 (62.81%), Postives = 158/199 (79.40%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCP+KL ELES++TL++P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPEPKGT 127
           +GITR  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  APEP G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFRENSWVSPNAVTRRR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 983.8 bits (2542), Expect = 5.6e-283
Identity = 506/506 (100.00%), Postives = 506/506 (100.00%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGE 240
           LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGE
Sbjct: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQGE 240

Query: 241 THPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTE 300
           THPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTE
Sbjct: 241 THPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVSTE 300

Query: 301 ASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLRV 360
           ASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLRV
Sbjct: 301 ASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKND 420
           RFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKND
Sbjct: 361 RFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKND 420

Query: 421 YFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDA 480
           YFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKRLIIHS 507
           FISLDTYDYQQSAGIRLVAKRLIIHS
Sbjct: 481 FISLDTYDYQQSAGIRLVAKRLIIHS 506

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_008453220.1 (PREDICTED: protein NEN1 [Cucumis melo])

HSP 1 Score: 911.4 bits (2354), Expect = 3.5e-261
Identity = 470/507 (92.70%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL D QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           E HPILSLVTCSSEDGA NLAE  ATESDSFNM T+SDQITG +LETDI+ME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSV FITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQK++SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 506

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_011660176.1 (protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucumis sativus])

HSP 1 Score: 896.0 bits (2314), Expect = 1.5e-256
Identity = 467/507 (92.11%), Postives = 482/507 (95.07%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISL-LDQG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL + QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           ETHPILSLVT  SED AS LAE  ATESDSFNM T SDQITG +LETDI+M EEHATVST
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINM-EEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSVSFITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEM+QKE+SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LII S
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIRS 505

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 891.3 bits (2302), Expect = 3.8e-255
Identity = 464/522 (88.89%), Postives = 485/522 (92.91%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNN--------------DTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG ++NN              +T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA+VSTEASP+E+TSTS+S+ TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAVSGD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. NCBI nr
Match: XP_022987423.1 (protein NEN1-like [Cucurbita maxima])

HSP 1 Score: 889.0 bits (2296), Expect = 1.9e-254
Identity = 465/522 (89.08%), Postives = 482/522 (92.34%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNN--------------DTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG + NN              +T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA VSTEASP+E+TSTSLSA TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAV GD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.7e-261
Identity = 470/507 (92.70%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL D QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           E HPILSLVTCSSEDGA NLAE  ATESDSFNM T+SDQITG +LETDI+ME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSV FITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQK++SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 506

BLAST of Bhi02G000208 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.4e-257
Identity = 467/507 (92.11%), Postives = 482/507 (95.07%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISL-LDQG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL + QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           ETHPILSLVT  SED AS LAE  ATESDSFNM T SDQITG +LETDI+M EEHATVST
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINM-EEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSVSFITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEM+QKE+SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LII S
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIRS 505

BLAST of Bhi02G000208 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 464/522 (88.89%), Postives = 485/522 (92.91%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNN--------------DTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG ++NN              +T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA+VSTEASP+E+TSTS+S+ TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAVSGD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 9.1e-255
Identity = 465/522 (89.08%), Postives = 482/522 (92.34%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG T DRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKL ELESYSTLVRPSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP+PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNN--------------DTHSSSS 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQG + NN              +T SSSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 RIGGNPISLLD-QGETHPILSLVTCSSEDGASNL-AESRATESDSFNMRTLSDQITGESL 300
           RIGG+PISL D QGE HPILSLVTCSSEDGASNL A+S ATESDSFNM TLSDQI GESL
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDISMEEEHATVSTEASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD SMEEEHA VSTEASP+E+TSTSLSA TEFLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSD 420
           SHKDDCLQLLCNNLRVRFGISTKFTDYAGRP+L+FVVDVPPSLC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPVVMRKNDYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFD 480
           SGSGSEWRPVVMRKN YFNYPTMRLHIPTAV GD+AIYATEMYQKE+SG AQRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPGAILDAFISLDTYDYQQSAGIRLVAKRLIIHS 507
           A ELDSLI PG I+DAFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Bhi02G000208 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.0e-250
Identity = 456/507 (89.94%), Postives = 473/507 (93.29%), Query Frame = 0

Query: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60
           MG TEDRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTLV+PSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           APEPKGTIDSLALLTQRFGRRAGDMK+ T  S         +SLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLH-ANSGDQSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLD-QG 240
           LFLESSLPEVF ENSWVSPNAVTRRR AKSSPQGNSSNN++H+SSS IGGNPISL D QG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300
           E HPILSLVTCSSEDGA NLAE  ATESDSFNM T+SDQITG +LETDI+ME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360
           EASPSEDTSTSL  TT+FLEPDQVSV FITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLH-TTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420
           VRFGISTKFTDYAGRP+LSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRP V RKN
Sbjct: 361 VRFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKN 420

Query: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQK++SGA QRLIFSKFDAAELDSLIKPGAILD
Sbjct: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 507
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G07710.11.7e-14958.02Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G61390.16.8e-14656.69Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G74390.14.2e-14353.53Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G74390.22.1e-13451.57Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G39810.13.6e-7062.81Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
Match NameE-valueIdentityDescription
Q9FLR02.4e-14858.02Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1[more]
Q0V8429.6e-14556.69Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1[more]
Q9CA745.8e-14253.53Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1[more]
F4JJ235.1e-6962.81Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038880150.15.6e-283100.00protein NEN1-like [Benincasa hispida][more]
XP_008453220.13.5e-26192.70PREDICTED: protein NEN1 [Cucumis melo][more]
XP_011660176.11.5e-25692.11protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucu... [more]
XP_022921575.13.8e-25588.89protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucu... [more]
XP_022987423.11.9e-25489.08protein NEN1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1S3BWV41.7e-26192.70protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1[more]
A0A0A0LSC37.4e-25792.11Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910... [more]
A0A6J1E6651.8e-25588.89protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1[more]
A0A6J1JJE49.1e-25589.08protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1[more]
A0A5A7UTA83.0e-25089.94Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 9..183
e-value: 4.2E-32
score: 122.6
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 12..174
e-value: 1.6E-23
score: 84.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 3..181
e-value: 1.6E-30
score: 108.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 292..311
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..232
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..311
NoneNo IPR availablePANTHERPTHR30231:SF27PROTEIN NEN3coord: 3..505
NoneNo IPR availablePANTHERPTHR30231DNA POLYMERASE III SUBUNIT EPSILONcoord: 3..505
NoneNo IPR availableCDDcd06127DEDDhcoord: 11..175
e-value: 1.7129E-28
score: 108.927
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 9..179

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000208Bhi02M000208mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0004386 helicase activity
molecular_function GO:0003676 nucleic acid binding