Bhi02G000197 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000197
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMitochondrial import inner membrane translocase subunit tim16
Locationchr2: 5427245 .. 5429607 (-)
RNA-Seq ExpressionBhi02G000197
SyntenyBhi02G000197
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTGGGTATGCCCCTGTTCTTAGATTCTTGTTTTTCTTTTTTTGTTTGTAGGGTTTACAAGAAACCTAACAGCTAAATAGCTGCTTTGATTGATTTTGCAGCTCTAGCGGCTTTTACGATTCAGGACCTGTTCTCAAGGCCAGCCAATTAATCATGGTATCTCTCTTCTTTCTTTCTCTCCTTCTGACTTGATGGAGGATTGATTTCTAAGATAATGAGAAATAAATATGGAATCAAGAGCAGATGTCTAAACTTCATTTATGTTAATTTTATTTGAATTATTATGTTTCTATCTCATCCATTAGGTAATTTGTTTCCACATTGATTATAGATTATGGGGTTTAATTCATAGTTTTCATTTCGTTTCCAATTTTTCTCTGGCCGAAGTTTTGGCCAGGGAAAAGACGAGAGAGACACAAGTCTTGTAGATATTTTCCTCATATTACATCCCATTTTCCTTTTTCCCTTTTTTCCCCTCCTCTTTGTGTCTGTTCTAGATATTTTGTGAAATAATGCTGACCTTTCTTGTCATCCCGCTTTCTAAAGCGTTTCAACTTAAGCATATTTGAAATGTATTTGTGTATCACTTGTGTCATGTTATCTTTTCTTCGACTGTACATTGTTTTTTTACCCATCAAATCAGTGCCATCTAATTAGAACTGGTGTATTTATTTCTGCTATGTCAATGTCTTTAGTTTTGATAGAGGAGAGTAGTTATTGCATAATTTTTAAATTGTCTTATTCAGTTTAAGAACTTCATATAAGAGAGCCTCTTTGCTTGTATGTATTGGACAGGCTGCAAAAATTCTTGCCAATTTAATTGTTATGGGCTCTGGGATACTTGCAAGGGCTTTTGTTCAAGCCTATCGTCAAGCCATTGCAAGTAAGTGCACACTTTTTATTCCACCATTTTTTTTTCCTTCTTTCTACTAGGTCCTATCGGAAGTTGGCCAATTTGTCGTCAATATCATGGTTATTGGAGTAAAATCTTTTTGGCGGGGTTGATGGGAACTTGATATATAGAACAAAAGAAACTTTCACATTGAACTATTAGAAGCTATAATGTACATGTGAGCTTGGGATGAACAAGCATTTTGCCAATGACAACAAAGTTCTTTATGCGCATAGTTTTATTCTGCTTTCAGATGATTGAAATTGGTATCAAAGCTTTTTTTTTTTCTTAATGTACTTGGGGAATGTTGACATCCTGAATTTCTATCTTTACATCCTTCCTCATCTTTGAAAGCCTGAATAATCTATTCTTAGATTTTCGACAAGTGGTCAAAAGAACTTTGTTATTTTTCTAGCCTGCGACATTGTCAAGCGTTTTGCAAATAAAAGCTAAGTTTTATATTGCGCATAGATTATAATTATTAGTCTTTTTGTTGACAAATAGATACAAAACCTTTTTGAGTGTTCATGACCTGTATGACAAGCACTTCCAACCTCTGGGCACCCTTAAAAGGCTGAACAATATCTTCAAGATCTTTGGACAAAATGCTTTTATTGGACATGTACTTTATTAGTTTGGACAAATTTGGTTGTTTATACAAAGTTTTTTTTTTGGCAAAATTACCTTCTTGATCCCTAAATTTCCACTTGGACAAATTTTGTTTGGGGGAAAATTTTCATTTGATCCCTAAATTTCAAAATGTTATAATTTTAGTTCTTAAATTTTGAGTTTGATTTCAATTTAGTCCCTAGGTTTCAAAATGATATAATTTTACCCTCGAGATTTGAGTTTTGTTTCAATTTGTCCCTAGGTTTCAAGATCTACACTTTTAACTTCAATGGTAAAATTATAACATTTTGAAAATTAAGGACTAAATTGAAACCAAATGCTAAACTCGAACTAAAAGAGTGACTTTTTGAATCCTACAAACCAAATAGAAATTAGACTTGAAACCTACGGACTAAGAAGATACTTTCTCCAAGCCTTTACTTAATGTGGGAGAATTTGACGTGGGTAGTTATAGATATACTCAATATCAAGTTTCTTTTATGTGTTGCAGATGCTGCAAAGTCCGGTGTTGCTCAAGAAACAATACAAAACACTGTTCGCAGAACAAGCAAAGTGATGACGGAGCAAGAAGCCAGGCAGATTCTCGGCGTCACCGAGGAAATGCCATGGGAGGAAGTTATGAAGGTCAAAATCCATTGATTTTCACTATAATATTTCTGGTCATTTATATATTATGAATAGGTTTTTGACAAACCTTCCTATTTGTGCAGAAATATGATGCTTTGTTTGAAAGGAATGCTCAAACTGGAAGCTTTTATCTTCAGTCAAAAGTTCACAGAGCCAAGGAACGATTAGAAACAATCTATCAGAGCAAAGGTCAAGATGCCCCTAGCTGA

mRNA sequence

ATGGGCTCTAGCGGCTTTTACGATTCAGGACCTGTTCTCAAGGCCAGCCAATTAATCATGGCTGCAAAAATTCTTGCCAATTTAATTGTTATGGGCTCTGGGATACTTGCAAGGGCTTTTGTTCAAGCCTATCGTCAAGCCATTGCAAATGCTGCAAAGTCCGGTGTTGCTCAAGAAACAATACAAAACACTGTTCGCAGAACAAGCAAAGTGATGACGGAGCAAGAAGCCAGGCAGATTCTCGGCGTCACCGAGGAAATGCCATGGGAGGAAGTTATGAAGAAATATGATGCTTTGTTTGAAAGGAATGCTCAAACTGGAAGCTTTTATCTTCAGTCAAAAGTTCACAGAGCCAAGGAACGATTAGAAACAATCTATCAGAGCAAAGGTCAAGATGCCCCTAGCTGA

Coding sequence (CDS)

ATGGGCTCTAGCGGCTTTTACGATTCAGGACCTGTTCTCAAGGCCAGCCAATTAATCATGGCTGCAAAAATTCTTGCCAATTTAATTGTTATGGGCTCTGGGATACTTGCAAGGGCTTTTGTTCAAGCCTATCGTCAAGCCATTGCAAATGCTGCAAAGTCCGGTGTTGCTCAAGAAACAATACAAAACACTGTTCGCAGAACAAGCAAAGTGATGACGGAGCAAGAAGCCAGGCAGATTCTCGGCGTCACCGAGGAAATGCCATGGGAGGAAGTTATGAAGAAATATGATGCTTTGTTTGAAAGGAATGCTCAAACTGGAAGCTTTTATCTTCAGTCAAAAGTTCACAGAGCCAAGGAACGATTAGAAACAATCTATCAGAGCAAAGGTCAAGATGCCCCTAGCTGA

Protein sequence

MGSSGFYDSGPVLKASQLIMAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS
Homology
BLAST of Bhi02G000197 vs. TAIR 10
Match: AT3G59280.1 (Protein Transporter, Pam16 )

HSP 1 Score: 158.3 bits (399), Expect = 4.3e-39
Identity = 81/116 (69.83%), Postives = 97/116 (83.62%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MA ++LANLIVMGSGI+ RA  QAYRQA+ANA+KSGVAQE +QN VR+  K +TEQEARQ
Sbjct: 1   MAGRLLANLIVMGSGIIGRAVFQAYRQALANASKSGVAQEAMQNGVRQAGKAITEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTE+  WEE+++KYD LFE NA+ GSFYLQSKVHRAKE LE +Y+S+G   PS
Sbjct: 61  ILGVTEKTSWEEILQKYDKLFENNAKAGSFYLQSKVHRAKECLEVVYRSQGNGTPS 116

BLAST of Bhi02G000197 vs. TAIR 10
Match: AT5G61880.1 (Protein Transporter, Pam16 )

HSP 1 Score: 136.7 bits (343), Expect = 1.4e-32
Identity = 75/108 (69.44%), Postives = 90/108 (83.33%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAA++LA++IVMGSGI+ARA  QAYRQA+ANA+K+GVA E  Q T++R    + E EARQ
Sbjct: 1   MAARVLASVIVMGSGIIARACTQAYRQALANASKTGVAHEATQ-TIKR-GLTIGEAEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQ 128
           ILGVTE+  W+E++KKYD LFERNAQ GSFYLQSKVHRAKE LET YQ
Sbjct: 61  ILGVTEKSSWDEILKKYDTLFERNAQNGSFYLQSKVHRAKECLETAYQ 106

BLAST of Bhi02G000197 vs. TAIR 10
Match: AT5G61880.2 (Protein Transporter, Pam16 )

HSP 1 Score: 136.7 bits (343), Expect = 1.4e-32
Identity = 75/108 (69.44%), Postives = 90/108 (83.33%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAA++LA++IVMGSGI+ARA  QAYRQA+ANA+K+GVA E  Q T++R    + E EARQ
Sbjct: 1   MAARVLASVIVMGSGIIARACTQAYRQALANASKTGVAHEATQ-TIKR-GLTIGEAEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQ 128
           ILGVTE+  W+E++KKYD LFERNAQ GSFYLQSKVHRAKE LET YQ
Sbjct: 61  ILGVTEKSSWDEILKKYDTLFERNAQNGSFYLQSKVHRAKECLETAYQ 106

BLAST of Bhi02G000197 vs. ExPASy Swiss-Prot
Match: Q93VV9 (Mitochondrial import inner membrane translocase subunit PAM16 like 2 OS=Arabidopsis thaliana OX=3702 GN=PAM16L2 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 6.1e-38
Identity = 81/116 (69.83%), Postives = 97/116 (83.62%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MA ++LANLIVMGSGI+ RA  QAYRQA+ANA+KSGVAQE +QN VR+  K +TEQEARQ
Sbjct: 1   MAGRLLANLIVMGSGIIGRAVFQAYRQALANASKSGVAQEAMQNGVRQAGKAITEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTE+  WEE+++KYD LFE NA+ GSFYLQSKVHRAKE LE +Y+S+G   PS
Sbjct: 61  ILGVTEKTSWEEILQKYDKLFENNAKAGSFYLQSKVHRAKECLEVVYRSQGNGTPS 116

BLAST of Bhi02G000197 vs. ExPASy Swiss-Prot
Match: Q93W66 (Mitochondrial import inner membrane translocase subunit PAM16 like 1 OS=Arabidopsis thaliana OX=3702 GN=PAM16L1 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 1.9e-31
Identity = 75/108 (69.44%), Postives = 90/108 (83.33%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAA++LA++IVMGSGI+ARA  QAYRQA+ANA+K+GVA E  Q T++R    + E EARQ
Sbjct: 1   MAARVLASVIVMGSGIIARACTQAYRQALANASKTGVAHEATQ-TIKR-GLTIGEAEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQ 128
           ILGVTE+  W+E++KKYD LFERNAQ GSFYLQSKVHRAKE LET YQ
Sbjct: 61  ILGVTEKSSWDEILKKYDTLFERNAQNGSFYLQSKVHRAKECLETAYQ 106

BLAST of Bhi02G000197 vs. ExPASy Swiss-Prot
Match: Q6PBL0 (Mitochondrial import inner membrane translocase subunit tim16 OS=Danio rerio OX=7955 GN=pam16 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.9e-11
Identity = 50/106 (47.17%), Postives = 66/106 (62.26%), Query Frame = 0

Query: 22  AKILANLIVMGSGILARAFVQAYRQ--AIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 81
           AK LA +IVMG+ ++ RAF +A RQ  A + AA     Q   Q+    +   MT QEA+Q
Sbjct: 2   AKYLAQIIVMGAQVVGRAFARALRQEFAASQAAAEARGQAGRQSAAASSFTGMTLQEAQQ 61

Query: 82  ILGVTEEMPWEEVMKKYDALFERN--AQTGSFYLQSKVHRAKERLE 124
           IL ++   P EE+ K Y+ LF+ N  A  GSFY+QSKV RAKERL+
Sbjct: 62  ILNISTLTP-EEIQKNYEHLFKVNDKAVGGSFYIQSKVVRAKERLD 106

BLAST of Bhi02G000197 vs. ExPASy Swiss-Prot
Match: Q9CQV1 (Mitochondrial import inner membrane translocase subunit TIM16 OS=Mus musculus OX=10090 GN=Pam16 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-10
Identity = 50/115 (43.48%), Postives = 70/115 (60.87%), Query Frame = 0

Query: 22  AKILANLIVMGSGILARAFVQAYRQ--AIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 81
           AK LA +IVMG  ++ RAF +A RQ  A + AA     +   Q+        ++ QEA+Q
Sbjct: 2   AKYLAQIIVMGVQVVGRAFARALRQEFAASQAAADARGRAGHQSAAASNLSGLSLQEAQQ 61

Query: 82  ILGVTEEMPWEEVMKKYDALFERNAQT--GSFYLQSKVHRAKERLETIYQSKGQD 133
           IL V++  P EEV K Y+ LF+ N ++  GSFYLQSKV RAKERL+   + + Q+
Sbjct: 62  ILNVSKLSP-EEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELRIQAQE 115

BLAST of Bhi02G000197 vs. ExPASy Swiss-Prot
Match: Q9Y3D7 (Mitochondrial import inner membrane translocase subunit TIM16 OS=Homo sapiens OX=9606 GN=PAM16 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 7.0e-10
Identity = 49/115 (42.61%), Postives = 70/115 (60.87%), Query Frame = 0

Query: 22  AKILANLIVMGSGILARAFVQAYRQ--AIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 81
           AK LA +IVMG  ++ RAF +A RQ  A + AA     +   ++        ++ QEA+Q
Sbjct: 2   AKYLAQIIVMGVQVVGRAFARALRQEFAASRAAADARGRAGHRSAAASNLSGLSLQEAQQ 61

Query: 82  ILGVTEEMPWEEVMKKYDALFERNAQT--GSFYLQSKVHRAKERLETIYQSKGQD 133
           IL V++  P EEV K Y+ LF+ N ++  GSFYLQSKV RAKERL+   + + Q+
Sbjct: 62  ILNVSKLSP-EEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELKIQAQE 115

BLAST of Bhi02G000197 vs. NCBI nr
Match: KAA0035623.1 (mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo var. makuwa] >TYK30919.1 mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo var. makuwa])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 120/133 (90.23%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 3   SSGFYDSGPVLKASQLIMAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQ 62
           SSGF DSG V++AS LIMAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+Q
Sbjct: 39  SSGFNDSGRVVRASHLIMAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQ 98

Query: 63  NTVRRTSKVMTEQEARQILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERL 122
           NTVRR SKVMTEQEARQILGVTEE PWEEV+KKYDALFERNAQTGSFYLQSKVHRAKERL
Sbjct: 99  NTVRRASKVMTEQEARQILGVTEETPWEEVVKKYDALFERNAQTGSFYLQSKVHRAKERL 158

Query: 123 ETIYQSKGQDAPS 136
           ET+YQ+KGQDAPS
Sbjct: 159 ETLYQNKGQDAPS 171

BLAST of Bhi02G000197 vs. NCBI nr
Match: XP_038878395.1 (mitochondrial import inner membrane translocase subunit PAM16 like 2-like [Benincasa hispida] >XP_038878396.1 mitochondrial import inner membrane translocase subunit PAM16 like 2-like [Benincasa hispida])

HSP 1 Score: 216.5 bits (550), Expect = 1.4e-52
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS
Sbjct: 61  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 116

BLAST of Bhi02G000197 vs. NCBI nr
Match: KAG6592401.1 (Mitochondrial import inner membrane translocase subunit PAM16 like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 214.5 bits (545), Expect = 5.5e-52
Identity = 113/128 (88.28%), Postives = 121/128 (94.53%), Query Frame = 0

Query: 3   SSGFYDSGPVLKASQLIMAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQ 62
           SSG  D GPV++ASQLIMAAKILANLIVMGSGILARAFVQAYRQA+ NA+KSGVAQETIQ
Sbjct: 72  SSGVNDFGPVIEASQLIMAAKILANLIVMGSGILARAFVQAYRQALTNASKSGVAQETIQ 131

Query: 63  NTVRRTSKVMTEQEARQILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERL 122
           NTVRR SKVMTEQEARQILGVT E PWEE++KKYDALFE+NAQTGSFYLQSKVHRAKERL
Sbjct: 132 NTVRRASKVMTEQEARQILGVTPETPWEEIVKKYDALFEKNAQTGSFYLQSKVHRAKERL 191

Query: 123 ETIYQSKG 131
           ET+YQSKG
Sbjct: 192 ETLYQSKG 199

BLAST of Bhi02G000197 vs. NCBI nr
Match: XP_004139541.1 (mitochondrial import inner membrane translocase subunit PAM16 like 2 [Cucumis sativus] >XP_011655013.1 mitochondrial import inner membrane translocase subunit PAM16 like 2 [Cucumis sativus] >KGN65052.1 hypothetical protein Csa_013024 [Cucumis sativus])

HSP 1 Score: 204.9 bits (520), Expect = 4.3e-49
Identity = 108/116 (93.10%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+QNTVRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQNTVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTEE PWEEV KKYDALFERNAQTGSFYLQSKVHRAKERLET+YQ+KGQDAPS
Sbjct: 61  ILGVTEETPWEEVAKKYDALFERNAQTGSFYLQSKVHRAKERLETLYQNKGQDAPS 116

BLAST of Bhi02G000197 vs. NCBI nr
Match: XP_008463624.1 (PREDICTED: mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo] >XP_008463625.1 PREDICTED: mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo] >XP_008463626.1 PREDICTED: mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo])

HSP 1 Score: 204.5 bits (519), Expect = 5.7e-49
Identity = 108/116 (93.10%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+QNTVRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQNTVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTEE PWEEV+KKYDALFERNAQTGSFYLQSKVHRAKERLET+YQ KGQDAPS
Sbjct: 61  ILGVTEETPWEEVVKKYDALFERNAQTGSFYLQSKVHRAKERLETLYQIKGQDAPS 116

BLAST of Bhi02G000197 vs. ExPASy TrEMBL
Match: A0A5D3E4Y7 (Mitochondrial import inner membrane translocase subunit tim16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G001310 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.2e-56
Identity = 120/133 (90.23%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 3   SSGFYDSGPVLKASQLIMAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQ 62
           SSGF DSG V++AS LIMAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+Q
Sbjct: 39  SSGFNDSGRVVRASHLIMAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQ 98

Query: 63  NTVRRTSKVMTEQEARQILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERL 122
           NTVRR SKVMTEQEARQILGVTEE PWEEV+KKYDALFERNAQTGSFYLQSKVHRAKERL
Sbjct: 99  NTVRRASKVMTEQEARQILGVTEETPWEEVVKKYDALFERNAQTGSFYLQSKVHRAKERL 158

Query: 123 ETIYQSKGQDAPS 136
           ET+YQ+KGQDAPS
Sbjct: 159 ETLYQNKGQDAPS 171

BLAST of Bhi02G000197 vs. ExPASy TrEMBL
Match: A0A0A0LW69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G185100 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.1e-49
Identity = 108/116 (93.10%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+QNTVRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQNTVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTEE PWEEV KKYDALFERNAQTGSFYLQSKVHRAKERLET+YQ+KGQDAPS
Sbjct: 61  ILGVTEETPWEEVAKKYDALFERNAQTGSFYLQSKVHRAKERLETLYQNKGQDAPS 116

BLAST of Bhi02G000197 vs. ExPASy TrEMBL
Match: A0A1S3CJP3 (mitochondrial import inner membrane translocase subunit tim16 OS=Cucumis melo OX=3656 GN=LOC103501727 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.7e-49
Identity = 108/116 (93.10%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ANA+KSGVAQET+QNTVRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALANASKSGVAQETMQNTVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVTEE PWEEV+KKYDALFERNAQTGSFYLQSKVHRAKERLET+YQ KGQDAPS
Sbjct: 61  ILGVTEETPWEEVVKKYDALFERNAQTGSFYLQSKVHRAKERLETLYQIKGQDAPS 116

BLAST of Bhi02G000197 vs. ExPASy TrEMBL
Match: A0A6J1EF52 (mitochondrial import inner membrane translocase subunit PAM16 like 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432719 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.0e-48
Identity = 106/116 (91.38%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ NA+KSGVAQETIQNTVRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALTNASKSGVAQETIQNTVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILGVT E PWEE++KKYDALFE+NAQTGSFYLQSKVHRAKERLET+YQSKGQDAPS
Sbjct: 61  ILGVTPETPWEEIVKKYDALFEKNAQTGSFYLQSKVHRAKERLETLYQSKGQDAPS 116

BLAST of Bhi02G000197 vs. ExPASy TrEMBL
Match: A0A6J1HHA5 (mitochondrial import inner membrane translocase subunit PAM16 like 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464057 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.8e-48
Identity = 105/116 (90.52%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 20  MAAKILANLIVMGSGILARAFVQAYRQAIANAAKSGVAQETIQNTVRRTSKVMTEQEARQ 79
           MAAKILANLIVMGSGILARAFVQAYRQA+ NA+KSGVAQETIQN+VRR SKVMTEQEARQ
Sbjct: 1   MAAKILANLIVMGSGILARAFVQAYRQALTNASKSGVAQETIQNSVRRASKVMTEQEARQ 60

Query: 80  ILGVTEEMPWEEVMKKYDALFERNAQTGSFYLQSKVHRAKERLETIYQSKGQDAPS 136
           ILG+T E PWEE++KKYDALFERNAQTGSFYLQSKVHRAKERLET+YQSKGQDAPS
Sbjct: 61  ILGITAETPWEEIVKKYDALFERNAQTGSFYLQSKVHRAKERLETLYQSKGQDAPS 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G59280.14.3e-3969.83Protein Transporter, Pam16 [more]
AT5G61880.11.4e-3269.44Protein Transporter, Pam16 [more]
AT5G61880.21.4e-3269.44Protein Transporter, Pam16 [more]
Match NameE-valueIdentityDescription
Q93VV96.1e-3869.83Mitochondrial import inner membrane translocase subunit PAM16 like 2 OS=Arabidop... [more]
Q93W661.9e-3169.44Mitochondrial import inner membrane translocase subunit PAM16 like 1 OS=Arabidop... [more]
Q6PBL04.9e-1147.17Mitochondrial import inner membrane translocase subunit tim16 OS=Danio rerio OX=... [more]
Q9CQV12.4e-1043.48Mitochondrial import inner membrane translocase subunit TIM16 OS=Mus musculus OX... [more]
Q9Y3D77.0e-1042.61Mitochondrial import inner membrane translocase subunit TIM16 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
KAA0035623.11.1e-5590.23mitochondrial import inner membrane translocase subunit tim16 [Cucumis melo var.... [more]
XP_038878395.11.4e-52100.00mitochondrial import inner membrane translocase subunit PAM16 like 2-like [Benin... [more]
KAG6592401.15.5e-5288.28Mitochondrial import inner membrane translocase subunit PAM16 like 2, partial [C... [more]
XP_004139541.14.3e-4993.10mitochondrial import inner membrane translocase subunit PAM16 like 2 [Cucumis sa... [more]
XP_008463624.15.7e-4993.10PREDICTED: mitochondrial import inner membrane translocase subunit tim16 [Cucumi... [more]
Match NameE-valueIdentityDescription
A0A5D3E4Y75.2e-5690.23Mitochondrial import inner membrane translocase subunit tim16 OS=Cucumis melo va... [more]
A0A0A0LW692.1e-4993.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G185100 PE=3 SV=1[more]
A0A1S3CJP32.7e-4993.10mitochondrial import inner membrane translocase subunit tim16 OS=Cucumis melo OX... [more]
A0A6J1EF521.0e-4891.38mitochondrial import inner membrane translocase subunit PAM16 like 2-like OS=Cuc... [more]
A0A6J1HHA51.8e-4890.52mitochondrial import inner membrane translocase subunit PAM16 like 2-like OS=Cuc... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 72..130
e-value: 9.6E-22
score: 78.5
NoneNo IPR availablePFAMPF03656Pam16coord: 20..130
e-value: 3.6E-23
score: 82.0
NoneNo IPR availablePANTHERPTHR12388:SF6MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT PAM16 LIKE 1coord: 20..129
IPR005341Mitochondrial import inner membrane translocase subunit Tim16PANTHERPTHR12388MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULEcoord: 20..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000197Bhi02M000197mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex