Bhi02G000180 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000180
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionSNAP25 homologous protein SNAP33
Locationchr2: 5043869 .. 5047874 (+)
RNA-Seq ExpressionBhi02G000180
SyntenyBhi02G000180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCTTCCTCTACTTTCACGCCAATTTCCATAGTTGTTTTGTCAAAGACTTCTTAAACCCCAAGTTGTTCCAATTCCTGCTGATCCTTTTGGCCCAGGTCTTCCTTTGACTCTTTCCCTCTTTCAGTTCCCTTCTTTCCTTCAAAACCCAGCACAAGTCTTCGCTGATTTATCAATTATACTCTCGATCCTCTTAGTTCTTCGTTTCTGAAGGTACATGATTGCTCCTTTCTCTCTAATTTTATCTGGGGTTTCTCTTGATTTCTTAATGCTTGTGGGTTTTTCTTTGAATTGAATGAGTGAACTCCCACTCGTTTTCTTGAGAAGCTTTACATTCGACGTTCTTCCTGCTTTGATTTTTATAATCTTTTTTTTTTTTTTTTTTTGTTAAATCTTATGCGATTTCTGAACTGATGATTTCACTGCGCACCAATGCCTGCTGGGTTTTAGAGCAAACTGAGCTTGTGTAAAAAGAAGAGGGGTAATCGGACTTTTATTATTGATTTGTCTGTTGTGGGATTTGGTATTGATGAACTAGCCATTTCAGGATTATCTCAGAGATGAATTTGGTTTTTAGGATATGTTTCATCAGGAATGGTGTTTTTTATTGCATACTGTTTGATTAAATGCCACGATTCATGTGGAAATATGATGTTCTGTTTAAGTTGTATCTCCCTGACTAAATCGATTTCATTATCACAGATTATGAGTTTTAAGAACCCTTCTGATCTTCTTGTACGTGTGATCCATTATTCTTTTTCGTCGAATTCTTCACTAGGAACAGCCTTTTTGATTGCATACTGTTTGATTAATTTCCATGATTCTTGTGGAAATATGGTGTCCTGTATAAGTTGTATTTTCCTGACTAACTTGATTTCATTACCACAGATCATGAGTCTCTATAACCCTTTTGATCTTCTTATGCTGATCTGTGTAATCCATTCTTCTTTTTCATCGAATTCTTCAACCAATTATTTGACTCTTTGCTTGGTTCCAGGGTGAAACTTTGTTAATTCTAAGCATTAGAATCATTGTTCTTATCACAAAACCATCAGCTGAATTACTGCATTTGCATTTCCAGAAATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCTCAAGCCAACCTGACAATTCTGCTCGTCCCAGTCCAAACCCAGTTGATTCAAATGATAATTTAGACAAGAAGCAAAAATACAAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGTAGCACCAAATCCCAGCTCCAATCTTTTCGACGATCATCTCGAACAAGAGACTAATTCTGCTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAAAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAGACAGTGGAGGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACGCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCCTGCATCAACAGGGTGAACAAATCACTCGGACCCACGTGGTTACTGCTGATATTGACAATGATCTTAGTCGGGTATGTGGAATGAATTAACCGCCATATAAACTATGATGAATGACATGTCGAGAGTGATTTTGAAATGGTTAAAATCACTTTTTGTCGTATTCAATAATCATTCAAAATTAGTTTCATGTTTGGTTACACTTTTAAATGTGGTTTTCATCCTATCAAAATTGACTTTAAACGATTAAAAACATGTCAAAATCACTCTTAAACCTGTTTTTTTCTTTCCTTATGTCCTCTTTGACTTGACTTGCGGAGAATCAAGGGATGTTTTAGCTTTTCTTAGCCTCCCTTCTTGTTGGACTCGTTGACCCTCACCCTATTTTCATCCTAATGAACTTACACATCATATAGAGGATGATTTGGGTCATGGTTTGTGTTAGCCTTAGTTCATGAAACCTAAAGGTGTGTAGATTGTGGGTTCATAATTTGTAAAAGTTGGTATATACTGTGAAATATGAGTTCTATAGGTCTAAGTTTATGAAATTACCAAGCCATGAATAAACAAACCTATGCACTCAATATAGGCTTAGCTTCATTTATGGATGCTTTTTGTAATGATATTGAAATGCTTTCGGTTATGATCATCAATATTTTATGATGTTGATTGCAGGGTGAGAAACTTCTTGGAAGCTTGGGAGGAATATTCTCTAAGACTTGGAAGCCAAAAAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGTAATATTTTCAAACTTTGGCGGTGGAAGAAAATAATTAAGAAAAATAGAACTGCTTTCTATAACTTTTTTTTTCTTCTTCTTTGTTTTATTATTTAATAATCTAAAAGTTTTACATGAAAATATACTTTTTTTTAATTAAATATTGATTTTTTATGTTATAAAATTTTCAAAAATAAATAAATAATACATTTATTTATGTTTTGGAAAATAATGAGAACAATTTTTTAAATGTTTATAAAAATTAAAAAAAAATAGAACACTTAATAAACACTTATGTCTCTAAAAAACTAGAAACAAGAAACATAAATATTATCTATATGATTTTAAATTATTTGAACTAATTTGAACAAGCTTAACCTATAGAACGAACAAGGTTTTAACTTTCCCACTAATCAGATGGATTAAGTTGATAACCAAGTAATTCATTAAAAATATTTTATAACATTTAATAAATAAATTTGAATTGGTTGCAGGAGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTTACGTGCCCCACATCCTGAGCCAACCAACTCCCTTAAGAAAGTTGAGGTCTGTAGTTATTCAGTTTTAGTGCTTCTCATGGAAGTACTTACAGAAAAAGCAATTCACTTACAAGCATTTTCCTCTCATACATAAACTAATATTTAAACTTGAAGGGGGCAGAATAGAAGAATATTTGCAAACACTTAACAAGAAATTTGTTCTAAATCAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAGGTGCGTTGTTATAGCCTTAGAGAATAAGTTGATTAGGATCGTTTGTAACAGGAAGTTTCTTGTCTTCAATTTTTGAAGAAATTGAAGTGTTTTTTTTTTTTTCTTAGAATCATTTCTCAAATTGCCTAGGAGAAATAGAAAAGATATGAATGAGAAATAAAAAAACGAAAATGTTACCAAATGGGTTGAAATGCCAATTTTTCTTCTGTATCTTTTATTTTTGTTTTTTATTTAACTTAGCTTGAATCAATGTCATTATTTCAGGCAAAATAAAGCTTTGGATCACTTGTTTGATGATGCCGACGAGGTCACGATTCGTGTTGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAGATTGCAGATTTGCAAGGTCTCATCTCATTTTTGCCATCAACAAATATGATGGTATATGCTTGACTGGATTGGCTTCCCTTCTGGTTTACAGTCTTTTCCTGTATGTCTTTCTTCGTCCAAAAAGAAACAAACATAATATATATATATTTTGGTCAAAACAGTTGTGAAATAGTTTGGTTATTGCAAGGGCAATCTGGTT

mRNA sequence

GGCTTCCTCTACTTTCACGCCAATTTCCATAGTTGTTTTGTCAAAGACTTCTTAAACCCCAAGTTGTTCCAATTCCTGCTGATCCTTTTGGCCCAGGTCTTCCTTTGACTCTTTCCCTCTTTCAGTTCCCTTCTTTCCTTCAAAACCCAGCACAAGTCTTCGCTGATTTATCAATTATACTCTCGATCCTCTTAGTTCTTCGTTTCTGAAGGGTGAAACTTTGTTAATTCTAAGCATTAGAATCATTGTTCTTATCACAAAACCATCAGCTGAATTACTGCATTTGCATTTCCAGAAATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCTCAAGCCAACCTGACAATTCTGCTCGTCCCAGTCCAAACCCAGTTGATTCAAATGATAATTTAGACAAGAAGCAAAAATACAAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGTAGCACCAAATCCCAGCTCCAATCTTTTCGACGATCATCTCGAACAAGAGACTAATTCTGCTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAAAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAGACAGTGGAGGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACGCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCCTGCATCAACAGGGTGAACAAATCACTCGGACCCACGTGGTTACTGCTGATATTGACAATGATCTTAGTCGGGGTGAGAAACTTCTTGGAAGCTTGGGAGGAATATTCTCTAAGACTTGGAAGCCAAAAAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGAGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTTACGTGCCCCACATCCTGAGCCAACCAACTCCCTTAAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAGGCAAAATAAAGCTTTGGATCACTTGTTTGATGATGCCGACGAGGTCACGATTCGTGTTGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAGATTGCAGATTTGCAAGGTCTCATCTCATTTTTGCCATCAACAAATATGATGGTATATGCTTGACTGGATTGGCTTCCCTTCTGGTTTACAGTCTTTTCCTGTATGTCTTTCTTCGTCCAAAAAGAAACAAACATAATATATATATATTTTGGTCAAAACAGTTGTGAAATAGTTTGGTTATTGCAAGGGCAATCTGGTT

Coding sequence (CDS)

ATGTCACCTTTTAGGAAATCCCCTATAAATGTTGCTAGGCACGGCTCAAGCCAACCTGACAATTCTGCTCGTCCCAGTCCAAACCCAGTTGATTCAAATGATAATTTAGACAAGAAGCAAAAATACAAGTCCTCAAGAATCACCTCTTCTCATCAGGCAACTGTAGCACCAAATCCCAGCTCCAATCTTTTCGACGATCATCTCGAACAAGAGACTAATTCTGCTTCATATTCTTCATCTTCAAGTTCATATTCATGGTCATCAAGAAACAGATACAAAAATGATTTCCGGGACTCAGGTGGGATTGAGAATCAGACAGTGGAGGAGTTGGAGAATTATGCTGTATACAAGGCTGAGGAGACAACTAACGCAGTCAACAACTGCCTGAAGATTGCTGAGGACATGAGAGAAGATGCAACCAAAACCTTAGTTGCCCTGCATCAACAGGGTGAACAAATCACTCGGACCCACGTGGTTACTGCTGATATTGACAATGATCTTAGTCGGGGTGAGAAACTTCTTGGAAGCTTGGGAGGAATATTCTCTAAGACTTGGAAGCCAAAAAAAGGTCGTCAGATAACAGGCCCTGTTATCTTTGAAGGAGATCAGGCTGTTATAAGCAAGGGCAACCATTTGGAGCAAAGGGAGAAGTTGGGGTTAAGTCGGGCACCAAATCCACGGTCCTTACGTGCCCCACATCCTGAGCCAACCAACTCCCTTAAGAAAGTTGAGGTGGAAAAGTTCAAGCAAGAGGATGCTCTTTCAGATTTGAGCAATATATTGGGAGAGCTTAAAGAAATGGCTGTTGATATGGGATCTGAACTAGACAGGCAAAATAAAGCTTTGGATCACTTGTTTGATGATGCCGACGAGGTCACGATTCGTGTTGATAATGCTAACCAGCGTGCTCGTCATCTGCTTAGAAAGTAG

Protein sequence

MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPSSNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEETTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNANQRARHLLRK
Homology
BLAST of Bhi02G000180 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 175/310 (56.45%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           M   RKSP N+ +H S    +S    PNP DS+D  D K     S+ T+S  +       
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSS---KPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     Q+ +S   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TT +V  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-LRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S  R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNA 300
            ++VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LDHL DD DE+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

Query: 301 NQRARHLLRK 310
           NQR R LL K
Sbjct: 301 NQRGRRLLGK 300

BLAST of Bhi02G000180 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 271.6 bits (693), Expect = 8.0e-73
Identity = 143/220 (65.00%), Postives = 168/220 (76.36%), Query Frame = 0

Query: 90  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNAVNNCLKIAEDMREDATKTLVALHQQ 149
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 150 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 209
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 210 NHLEQREKLGLSRAPNPRSLRAPHPEPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAV 269
           NH E+REKLGL  A    S +    +PTN+L+KVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 270 DMGSELDRQNKALDHLFDDADEVTIRVDNANQRARHLLRK 310
           DMGSE+D+QNKALDHL DD DE+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

BLAST of Bhi02G000180 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 231.1 bits (588), Expect = 1.2e-60
Identity = 132/252 (52.38%), Postives = 173/252 (68.65%), Query Frame = 0

Query: 58  NPSSNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 117
           N S N FDD  E   + +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 118 AEETTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSL 177
           +EETT  V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 178 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPT 237
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 238 NSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVD 297
           ++ +K ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LDHL D+ADE+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 298 NANQRARHLLRK 310
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

BLAST of Bhi02G000180 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 43.9 bits (102), Expect = 2.7e-04
Identity = 26/70 (37.14%), Postives = 35/70 (50.00%), Query Frame = 0

Query: 235 EPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTI 294
           E +   ++ E+ K KQE  L  +S  L  LK MA DM  ELDRQ   +D +    D  T 
Sbjct: 163 ESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELDRQVPLMDEIDTKVDRATS 222

Query: 295 RVDNANQRAR 305
            + N N R +
Sbjct: 223 DLKNTNVRLK 232

BLAST of Bhi02G000180 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-80
Identity = 175/310 (56.45%), Postives = 216/310 (69.68%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           M   RKSP N+ +H S    +S    PNP DS+D  D K     S+ T+S  +       
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSS---KPNPFDSDDESDNKHTLNPSKRTTSEPSLA---DM 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           +N F     Q+ +S   SSS  S   +S+ +YKN+FRDSGGIENQ+V+ELE YAVYKAEE
Sbjct: 61  TNPFGGERVQKGDS---SSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TT +V  CLK+AED+R DAT+TLV LH QGEQITRTH    +ID+DLSRGEKLLGSLGG+
Sbjct: 121 TTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGM 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRS-LRAPHPEPTNS 240
           FSKTWKPKK R I GPV+   D +   + NHLE+REKLGL+ AP  +S  R P PE  ++
Sbjct: 181 FSKTWKPKKTRPINGPVVTR-DDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 LKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNA 300
            ++VE+EK KQ+D LSDLS+ILGELK MAVDMGSE+++QNK LDHL DD DE+  RV  +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 300

Query: 301 NQRARHLLRK 310
           NQR R LL K
Sbjct: 301 NQRGRRLLGK 300

BLAST of Bhi02G000180 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.1e-71
Identity = 143/220 (65.00%), Postives = 168/220 (76.36%), Query Frame = 0

Query: 90  NRYKNDFRDSGGIENQTVEELENYAVYKAEETTNAVNNCLKIAEDMREDATKTLVALHQQ 149
           ++YKN F DSGG+++QT EELE YAVYKAEETT  VNNCLKIAED+R D  +TL  LHQQ
Sbjct: 46  DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQ 105

Query: 150 GEQITRTHVVTADIDNDLSRGEKLLGSLGGIFSKTWKPKKGRQITGPVIFEGDQAVISKG 209
           GEQI RTH +  D+D DLSRGEKLL +LGG+FSK WKPKK + ITGP+I   D+      
Sbjct: 106 GEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKPWKPKKTKNITGPMI-TPDKPSKKSE 165

Query: 210 NHLEQREKLGLSRAPNPRSLRAPHPEPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAV 269
           NH E+REKLGL  A    S +    +PTN+L+KVE EK KQ+D LSDLS+ILG+LK MAV
Sbjct: 166 NHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAV 225

Query: 270 DMGSELDRQNKALDHLFDDADEVTIRVDNANQRARHLLRK 310
           DMGSE+D+QNKALDHL DD DE+  RV  ANQRARHLL K
Sbjct: 226 DMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARHLLSK 263

BLAST of Bhi02G000180 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-59
Identity = 132/252 (52.38%), Postives = 173/252 (68.65%), Query Frame = 0

Query: 58  NPSSNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYK 117
           N S N FDD  E   + +               R+ +  + SGG ENQTV+ELE+YAVY 
Sbjct: 5   NSSWNPFDDEKEAAKSFSLNPFDDDDDDKEVEKRFTSSLKPSGGKENQTVQELESYAVYN 64

Query: 118 AEETTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSL 177
           +EETT  V  CLK+AE++R DA+KTLV L++QG+QITRTH  T D+D+ LSRGEK+LG L
Sbjct: 65  SEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRL 124

Query: 178 GGIFSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPT 237
           GG+FS+TWKPKK R ITGPVI +GD     K   L+ REKLGL+ +  P+S     PE  
Sbjct: 125 GGVFSRTWKPKKSRSITGPVITKGDSPK-RKVIDLKTREKLGLNPSLKPKSKTL--PEAV 184

Query: 238 NSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVD 297
           ++ +K ++   KQ++AL+DLS +LGELK MAVDMG+ ++RQ   LDHL D+ADE+  RV 
Sbjct: 185 DAYQKTQIA--KQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVK 244

Query: 298 NANQRARHLLRK 310
            +NQRAR+LLRK
Sbjct: 245 QSNQRARYLLRK 251

BLAST of Bhi02G000180 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.7e-15
Identity = 61/213 (28.64%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 107 VEELENYAVYKAEETTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 166
           +EE++  A   A+E+  +    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 167 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 226
           +   EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 227 REKLGLSRAPNPRSLRAPHPEPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 286
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 287 LDRQNKALDHLFDDADEVTIRVDNANQRARHLL 308
           +D QN+ +D + + AD    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

BLAST of Bhi02G000180 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 9.7e-15
Identity = 61/213 (28.64%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 107 VEELENYAVYKAEETTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDND 166
           +EE++  A   A+E+  +    L++ E+ ++   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 167 LSRGEKLLGSLGGI------------FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQ 226
           +   EK L  LG               S  +K   G    G V  +  + V       ++
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVV-------DE 130

Query: 227 REKLGLSRAPNPRSLRAPHPEPTNSLKKVEVEKFKQEDALSDLSNILGELKEMAVDMGSE 286
           RE++ +S     R         TN  ++ E+     ++ L  +S I+G L+ MA+DMG+E
Sbjct: 131 REQMAISGGFIRR--------VTNDARENEM-----DENLEQVSGIIGNLRHMALDMGNE 190

Query: 287 LDRQNKALDHLFDDADEVTIRVDNANQRARHLL 308
           +D QN+ +D + + AD    R+D ANQRA  +L
Sbjct: 191 IDTQNRQIDRIMEKADSNKTRIDEANQRATKML 203

BLAST of Bhi02G000180 vs. NCBI nr
Match: XP_038879751.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879753.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 597.0 bits (1538), Expect = 9.0e-167
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS
Sbjct: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN
Sbjct: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 309

BLAST of Bhi02G000180 vs. NCBI nr
Match: XP_004138184.1 (SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical protein Csa_013922 [Cucumis sativus])

HSP 1 Score: 547.4 bits (1409), Expect = 8.2e-152
Identity = 286/309 (92.56%), Postives = 298/309 (96.44%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINV RHGSSQP NSAR SPNPV+SNDNLDKKQK +SSRITSSHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLE+ T+S+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALDHLFDDA+EVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 308

BLAST of Bhi02G000180 vs. NCBI nr
Match: XP_008453247.1 (PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.1 putative SNAP25-like proteinous protein SNAP30 [Cucumis melo var. makuwa])

HSP 1 Score: 528.1 bits (1359), Expect = 5.1e-146
Identity = 279/309 (90.29%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINV RH S+QP N AR SPN V+SNDNLDK+QK +SSRITSSHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLE+ T+S SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALD+LFDDA+EVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of Bhi02G000180 vs. NCBI nr
Match: XP_022921394.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921397.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 526.6 bits (1355), Expect = 1.5e-145
Identity = 273/309 (88.35%), Postives = 290/309 (93.85%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MS FRKSPI  ARHGSSQP N AR +  P+DSNDNLDK+QK+KSSRITSSHQATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNAVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDADE+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 310
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of Bhi02G000180 vs. NCBI nr
Match: XP_023516514.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516515.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_023516516.1 putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 3.3e-145
Identity = 272/309 (88.03%), Postives = 289/309 (93.53%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MS FRKSPI  ARHGSSQP N AR    P+DSNDNLDK+QK+KSSRITSSHQATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSPSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNAVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGP+IFEGDQAVISKGNHLEQRE+LGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPIIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDADE+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 310
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of Bhi02G000180 vs. ExPASy TrEMBL
Match: A0A0A0LRS5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G012100 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 3.9e-152
Identity = 286/309 (92.56%), Postives = 298/309 (96.44%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINV RHGSSQP NSAR SPNPV+SNDNLDKKQK +SSRITSSHQAT APNP 
Sbjct: 1   MSPFRKSPINVGRHGSSQPANSARSSPNPVNSNDNLDKKQK-QSSRITSSHQATAAPNPK 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLE+ T+S+SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELE+YAVYKAEE
Sbjct: 61  SALFDDHLEKGTDSSSYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELESYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQ+DALSDLSNILGELKEMAVDMGSELD+QNKALDHLFDDA+EVTIRVDNAN
Sbjct: 241 QKVEVEKVKQDDALSDLSNILGELKEMAVDMGSELDKQNKALDHLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 308

BLAST of Bhi02G000180 vs. ExPASy TrEMBL
Match: A0A5A7US66 (Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002820 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.5e-146
Identity = 279/309 (90.29%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINV RH S+QP N AR SPN V+SNDNLDK+QK +SSRITSSHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLE+ T+S SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALD+LFDDA+EVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of Bhi02G000180 vs. ExPASy TrEMBL
Match: A0A1S3BVT2 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103494024 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.5e-146
Identity = 279/309 (90.29%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MSPFRKSPINV RH S+QP N AR SPN V+SNDNLDK+QK +SSRITSSHQ T APNP 
Sbjct: 1   MSPFRKSPINVVRHSSNQPANPARSSPNLVNSNDNLDKRQK-QSSRITSSHQETAAPNPK 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           S LFDDHLE+ T+S SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE
Sbjct: 61  SALFDDHLEKGTDS-SYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTN+VNNCLKIAED+REDATKTLVALHQQGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNSVNNCLKIAEDIREDATKTLVALHQQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGD AVISKGNHLEQREKLGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDHAVISKGNHLEQREKLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELKEMAVDMGSELD+QN+ALD+LFDDA+EVTIRVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKEMAVDMGSELDKQNRALDNLFDDANEVTIRVDNAN 300

Query: 301 QRARHLLRK 310
           QRARHLLRK
Sbjct: 301 QRARHLLRK 307

BLAST of Bhi02G000180 vs. ExPASy TrEMBL
Match: A0A6J1E197 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111429687 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 7.2e-146
Identity = 273/309 (88.35%), Postives = 290/309 (93.85%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MS FRKSPI  ARHGSSQP N AR +  P+DSNDNLDK+QK+KSSRITSSHQATVAPNPS
Sbjct: 1   MSHFRKSPIRFARHGSSQPANPARSTSQPIDSNDNLDKRQKHKSSRITSSHQATVAPNPS 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNAVNNCLKIAEDMREDAT TLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATNTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALD+LFDDADE+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDNLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 310
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

BLAST of Bhi02G000180 vs. ExPASy TrEMBL
Match: A0A6J1JJU0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111485094 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.1e-145
Identity = 272/309 (88.03%), Postives = 288/309 (93.20%), Query Frame = 0

Query: 1   MSPFRKSPINVARHGSSQPDNSARPSPNPVDSNDNLDKKQKYKSSRITSSHQATVAPNPS 60
           MS FRKSPI  ARHGSSQP   AR +  P+DSNDN DK+QK+KSSRITSSHQATVAPNP 
Sbjct: 1   MSHFRKSPIRFARHGSSQPAYPARSTSQPIDSNDNSDKRQKHKSSRITSSHQATVAPNPI 60

Query: 61  SNLFDDHLEQETNSASYSSSSSSYSWSSRNRYKNDFRDSGGIENQTVEELENYAVYKAEE 120
           SNLFDDHLE+ET+    SSS SSYSWSSRNRYKNDFRDSGG+++QTVEELENYA YKAEE
Sbjct: 61  SNLFDDHLEKETD----SSSHSSYSWSSRNRYKNDFRDSGGLQSQTVEELENYAAYKAEE 120

Query: 121 TTNAVNNCLKIAEDMREDATKTLVALHQQGEQITRTHVVTADIDNDLSRGEKLLGSLGGI 180
           TTNAVNNCLKIAEDMREDATKTLVALH QGEQITRTH+VTADIDNDLSRGEKLLGSLGGI
Sbjct: 121 TTNAVNNCLKIAEDMREDATKTLVALHHQGEQITRTHMVTADIDNDLSRGEKLLGSLGGI 180

Query: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQREKLGLSRAPNPRSLRAPHPEPTNSL 240
           FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRE+LGLSRAPNPRS RAPHPEPTNSL
Sbjct: 181 FSKTWKPKKGRQITGPVIFEGDQAVISKGNHLEQRERLGLSRAPNPRSSRAPHPEPTNSL 240

Query: 241 KKVEVEKFKQEDALSDLSNILGELKEMAVDMGSELDRQNKALDHLFDDADEVTIRVDNAN 300
           +KVEVEK KQEDALSDLSNILGELK MAVDMG+ELDRQNKALDHLFDDADE+T+RVDNAN
Sbjct: 241 QKVEVEKVKQEDALSDLSNILGELKGMAVDMGTELDRQNKALDHLFDDADEITVRVDNAN 300

Query: 301 QRARHLLRK 310
           +RARHLLRK
Sbjct: 301 RRARHLLRK 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G61210.11.6e-8156.45soluble N-ethylmaleimide-sensitive factor adaptor protein 33 [more]
AT1G13890.18.0e-7365.00soluble N-ethylmaleimide-sensitive factor adaptor protein 30 [more]
AT5G07880.11.2e-6052.38synaptosomal-associated protein SNAP25-like 29 [more]
AT3G09740.12.7e-0437.14syntaxin of plants 71 [more]
Match NameE-valueIdentityDescription
Q9S7P92.3e-8056.45SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ... [more]
Q9LMG81.1e-7165.00Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA... [more]
Q9SD961.7e-5952.38SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ... [more]
Q17QQ39.7e-1528.64Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1[more]
P608789.7e-1528.64Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038879751.19.0e-167100.00SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038879752.1 SNAP25... [more]
XP_004138184.18.2e-15292.56SNAP25 homologous protein SNAP33 [Cucumis sativus] >KGN63709.1 hypothetical prot... [more]
XP_008453247.15.1e-14690.29PREDICTED: putative SNAP25 homologous protein SNAP30 [Cucumis melo] >KAA0057978.... [more]
XP_022921394.11.5e-14588.35putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022921396.1 p... [more]
XP_023516514.13.3e-14588.03putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo] >XP_02351... [more]
Match NameE-valueIdentityDescription
A0A0A0LRS53.9e-15292.56t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A5A7US662.5e-14690.29Putative SNAP25-like proteinous protein SNAP30 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BVT22.5e-14690.29putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10349402... [more]
A0A6J1E1977.2e-14688.35putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JJU06.1e-14588.03putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..149
NoneNo IPR availableCOILSCoilCoilcoord: 289..309
NoneNo IPR availableGENE3D1.20.5.110coord: 101..178
e-value: 1.3E-19
score: 71.9
NoneNo IPR availableGENE3D1.20.5.110coord: 242..307
e-value: 4.7E-21
score: 76.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..89
NoneNo IPR availablePANTHERPTHR19305SYNAPTOSOMAL ASSOCIATED PROTEINcoord: 22..309
NoneNo IPR availablePANTHERPTHR19305:SF30TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 22..309
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 249..305
e-value: 1.6945E-13
score: 62.1908
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 249..307
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 105..175
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 239..306
e-value: 1.4E-8
score: 44.5
coord: 110..177
e-value: 13.0
score: 2.8
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 249..306
score: 14.563452
IPR044766NPSN/SNAP25-like, N-terminal SNARE domainCDDcd15861SNARE_SNAP25N_23N_29N_SEC9Ncoord: 112..176
e-value: 2.52498E-20
score: 81.0803

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000180Bhi02M000180mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061025 membrane fusion
biological_process GO:0015031 protein transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005484 SNAP receptor activity