Bhi02G000066 (gene) Wax gourd (B227) v1

Overview
NameBhi02G000066
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionO-phosphoserine phosphohydrolase
Locationchr2: 1900569 .. 1904611 (-)
RNA-Seq ExpressionBhi02G000066
SyntenyBhi02G000066
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTTTTTAAAATTTCCCCTAAATAGGGCCATCCGTACGAATTTCACAGTCGAAGGTGGAGCATTAGTTTCGCGGACAAAAAGAACACAGATCTAGAGAAGAGAGAACACACAACGGTAAATTTTCTGATCCTTTGTCTCTGTATTCCACATTTTTCTTTCTCTAATCAATCTTAATCCGTCGTGCTTTGCATATCCCATAATCGGCCTTCCATTTTCCTTTGTTCTTTGTTCATTTTTCACTCACATACGTTTTAGTTTCCTCTTGGAAATTGTTTATGGGGGAAGATGTGTGAAAACCCCTGGAAAGTGAAGCTTAGTATTGAGTGTTGTTCAATTTCTGCAGCTGGTTTGAAATTTATCTTCCTACTCCTTTTCTTATTGTTATTTGTGATTTTGTTTTGGATTTTTATGACAAATTTATGTTTTCTTTTGTATTTTCTGAGTTCATGTAGATCTAAATTCCTTAAATTGTACTATATTACATAAATATCATATATTATCGTGTTGATTATTGATTTAGAGACTTCTTTTGATGGATTTTCTTTGATTTAATTATGAACTGGGTTAGTGTTGGAGTGTATGTGTCGACCAGGTTCACTGATTTGGATACTTTATTTATGTTCATGACAGATAATTGATGGAAAGTTTGTTGAGTGCACGAATCAAACCATTACCTACTCATTGTAGAAAAATTCATTCCTTATTTCGTCCTAAGTTGTTCTTGCAATTTTCTAGAAGATTGGATAAATGTCATATGGAGATGGGGAAATGTCGTACGTCATTCAATCCATTTGCCGCATCAGTTCAGCCAATAGATAAACTTTCTCAATTCAACAACACATCACCATCCAAAGGTCACTACAAATTTCTTGAATATATTGTCCTCTTCAGTGTCTGTACCGTCTATCTACTATGTGGGGATGGAATCTCATCAACATGATAATATCTTTTTGGTAGCACCCTACATAGTGTAGAGTCGGTGGTTTGCAAATACAGGTGGAAGGGAGATTATCTCTAGCTTCTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTTTGAGGTTAATTGTACTATGAAGGGGAAAAAATGGAAATGTGATTTTTTCGGCAATCAGTGAAATAATTGGGCCTGAGCTTTATGGACATTTTCAAATGAGTTAATGGTGGCCACAGCTGAGTTATTTTACCCAATGATCAAAATTTCTTGTTACTGAGTTCCTTAACTCAATCTTGTTATATATTCTTTGATATCTCTTTGTTGGTTAGTGGTCTTAAATTTTACTCTCAAAATATCAGAGGTGTTGGAACTGTGGAGAGGTGCAAATGCAGTATGCTTCGATGTTGATAGCACAGTTTGTGTAGACGAGGGCATTGATGAATTAGCAGATTTCTGTGGATCCGGAGAGGCGGTTGCAGAATGGACTGCTAGGTTTGATTTGCTTGTTTTTTTGCATGATTTAATTGGCATCCATGAATTTTCATTAACTGAAATTTTTAAGTCTGGTCCAGGGCAATGGGTGGTTCTGTTCCATTTGAGGATGCCTTGGCTGCTAGACTATCTTTGTTTAACCCTTCTCTTTCACAAGTTGAGGAATTTCTTGCTAAGAGACCTCCAAGGTATGGAGCTATGTCCACGTGCATTAAAGTTATCCATTAACCATGGAAGTATACCAAAAAAGGGTTGGGGGGATTATGGGCAAGGCATACATGAGTCCATGACTACAGAACATTTGTGTATTCTGATAGAGACTTGGTGCTTTCTGTTTCTAAGACTTAAAAAGGTTGCAAAAGATACACATACTTGGATCGTCTGGCTAGATCAAATGATGTTGGTGTTATCATCTTCCTTTCTGTCTTGTTCTTAACATAATGCGAGATGGTTATTTTACTTTTCTATCTTGTAGATAGCTGAATCTTTAGACCACTAATGTTATTAATATGGGACCATTCAACTCCAAGGGCACTCAGATATTGTTTCTGATATTAACAGCTGCAAGTTTGTTTGTCTCAATATTTTTACTTTAACAGCCGCCTGTTCCATTTCGATTGTAAATAAGTCTTCAGAGCAAACTTTATAGGATGTCTGAATTACTTACTCTGGGAAGATGCATTATTATAACAACCAGACTGACCAAACCTCCACAAAAGAATGGACATGAAAACTTTTCATTGAATGTGAAGTATTTTAAGTGGTAATTCTTCAAACAAGACTTTGCATTTGCTGTAAAATGTATATCTGAAAACAGTATAAGACCATCTGTGCTTCAAAATCTTCCAGCTGACTCATTAAAGGTAGGATTAAAGAGCAGAGAGATGTTTTTTTGGCTCTTTTTGTTATTCCTCCAGCTTATCCCGTTAAAAGTTTTCTAGTCCTATTTTTCATAGTGCTGATTGTTACTCTGTAGGCTTTCTCCAGGTATTGATGAACTAGTTAAAAAGCTAAAAGCTAACAACATTGATGTATATCTGATTTCGGGAGGCTTTCGTCAAATGATCAATGTAAGTTTATGGCTACTTGTAGTGAGGCCCTCATGTTTGTTATGTTACATTCATTTCATCAAGGACTAAATCAATTTCTGATATCAGTTATAGGAAAATTAATGAGGCATTGATGTTCATTATTATTAATCAACGAGCTACTTTATGTGAAGCCTGTTGCATCAATTCTTGGGATTCCACATGAGAATATTTTTGCCAACCAATTGCTTTTTGGAAGCAATGGTGAATTTATGGGGTTTGACAAGAATGAGCCTACTTCAAGGAGTGGGGGAAAAGCAATTGCAGTACAACAATTGAGGAAGGTAGAATAATTGCTTTTGGTAAGACATTCTGTTGATTTTTCACATACAAGTTTTAATTCGGGTTGATTTTACAGGCTCGTGGATACAAGACATTGGTCATGACTGGGGACGGTGCAACTGATCTTGAGGTGAGAGGAGACATTCGATCCTCGAACTGTAAATTGCGGCAATTTTCTTCTGAAATATCCTAAAATGCTAAACAGTGGTACCTATTCCTAGCTAAACCTTCATAGGCAAATAAATGACTTCCACTCCTAGCTTCATAATTATGGCTTGATGCTTACATTTTGTTTCTTCGTGGTTTTAATTGATTTGAGATGTTTATATTCCAACTAATTTGAAAACTCCTAGAGCCAATTGAGCTGAATGTGCAAACAAGTTGAGCCTGGTTTTGTTAACCACTTGGGGGAGTTTGGAGCGATGAATTGGTTACTAGAGTAAGTGGTTATTATAGGCTATGGGTTTCTTTTAATGAAATTATAGTCTATAGGTTATAATAGTACGTGTTTGGGATGTAGACTATTTTAGTCTAAGTGGGAAATAATAAACATTGTAACAAAGAGGAGAAGGAAATAATAAACAATGTGACAAAGAGGGATTTGAAATAGTAATATCGTAGTTAAAATATTGAAAACACCAACTATTATATAATTGGATTCCCAAACATGAAGTAGGCTATAATAGCTCACTCCACTCATTTTGAAGTTAGGGGCCGGACAACCCCTTATATTTGCAAGCAAGTTGCAGCTTGTTCATTACTGTACTTCTAATGCACATTCTTTATTCTTTCGGTTTAGGCCTGTAAACCAGGAGGCGCTGACTTGTTTATATGCTATGCTGGCGTTCAACTTCGAGAAAATGTCGCTGCAAAAGCTGACTGGCTGGTTTTCAGTTTTGTAGATATAATCAGCTCCCTTGACTAACTCGTATTTTTTCAGAACTGCCCTCATTTTGATTTTTATATCTTTTTTGTTATTCTTATCTTGTATTGCTTGCCTATTCTTTATGGCTGCAGACATTTGCATTCTACGCATAACTATTCTAATCTTTTGGGGAAAAAGAGCCTCCATTATCATGTTTGTGAAACTGAAAAGTATAAACAAGGATTGGAATTCTGAAGCATCTTTCTTCTCAATACACAAAATTTACCTTTTTACAGTTGTAAAATGCTCCCACTATGAGGGTGAAAATTGAATTTAC

mRNA sequence

GGTTTTTTAAAATTTCCCCTAAATAGGGCCATCCGTACGAATTTCACAGTCGAAGGTGGAGCATTAGTTTCGCGGACAAAAAGAACACAGATCTAGAGAAGAGAGAACACACAACGGTAAATTTTCTGATCCTTTGTCTCTGTATTCCACATTTTTCTTTCTCTAATCAATCTTAATCCGTCGTGCTTTGCATATCCCATAATCGGCCTTCCATTTTCCTTTGTTCTTTGTTCATTTTTCACTCACATACGTTTTAGTTTCCTCTTGGAAATTGTTTATGGGGGAAGATGTGTGAAAACCCCTGGAAAGTGAAGCTTAGTATTGAGTGTTGTTCAATTTCTGCAGCTGATAATTGATGGAAAGTTTGTTGAGTGCACGAATCAAACCATTACCTACTCATTGTAGAAAAATTCATTCCTTATTTCGTCCTAAGTTGTTCTTGCAATTTTCTAGAAGATTGGATAAATGTCATATGGAGATGGGGAAATGTCGTACGTCATTCAATCCATTTGCCGCATCAGTTCAGCCAATAGATAAACTTTCTCAATTCAACAACACATCACCATCCAAAGAGGTGTTGGAACTGTGGAGAGGTGCAAATGCAGTATGCTTCGATGTTGATAGCACAGTTTGTGTAGACGAGGGCATTGATGAATTAGCAGATTTCTGTGGATCCGGAGAGGCGGTTGCAGAATGGACTGCTAGGGCAATGGGTGGTTCTGTTCCATTTGAGGATGCCTTGGCTGCTAGACTATCTTTGTTTAACCCTTCTCTTTCACAAGTTGAGGAATTTCTTGCTAAGAGACCTCCAAGGCTTTCTCCAGGTATTGATGAACTAGTTAAAAAGCTAAAAGCTAACAACATTGATGTATATCTGATTTCGGGAGGCTTTCGTCAAATGATCAATCCTGTTGCATCAATTCTTGGGATTCCACATGAGAATATTTTTGCCAACCAATTGCTTTTTGGAAGCAATGGTGAATTTATGGGGTTTGACAAGAATGAGCCTACTTCAAGGAGTGGGGGAAAAGCAATTGCAGTACAACAATTGAGGAAGGCTCGTGGATACAAGACATTGGTCATGACTGGGGACGGTGCAACTGATCTTGAGGCCTGTAAACCAGGAGGCGCTGACTTGTTTATATGCTATGCTGGCGTTCAACTTCGAGAAAATGTCGCTGCAAAAGCTGACTGGCTGGTTTTCAGTTTTGTAGATATAATCAGCTCCCTTGACTAACTCGTATTTTTTCAGAACTGCCCTCATTTTGATTTTTATATCTTTTTTGTTATTCTTATCTTGTATTGCTTGCCTATTCTTTATGGCTGCAGACATTTGCATTCTACGCATAACTATTCTAATCTTTTGGGGAAAAAGAGCCTCCATTATCATGTTTGTGAAACTGAAAAGTATAAACAAGGATTGGAATTCTGAAGCATCTTTCTTCTCAATACACAAAATTTACCTTTTTACAGTTGTAAAATGCTCCCACTATGAGGGTGAAAATTGAATTTAC

Coding sequence (CDS)

ATGGAAAGTTTGTTGAGTGCACGAATCAAACCATTACCTACTCATTGTAGAAAAATTCATTCCTTATTTCGTCCTAAGTTGTTCTTGCAATTTTCTAGAAGATTGGATAAATGTCATATGGAGATGGGGAAATGTCGTACGTCATTCAATCCATTTGCCGCATCAGTTCAGCCAATAGATAAACTTTCTCAATTCAACAACACATCACCATCCAAAGAGGTGTTGGAACTGTGGAGAGGTGCAAATGCAGTATGCTTCGATGTTGATAGCACAGTTTGTGTAGACGAGGGCATTGATGAATTAGCAGATTTCTGTGGATCCGGAGAGGCGGTTGCAGAATGGACTGCTAGGGCAATGGGTGGTTCTGTTCCATTTGAGGATGCCTTGGCTGCTAGACTATCTTTGTTTAACCCTTCTCTTTCACAAGTTGAGGAATTTCTTGCTAAGAGACCTCCAAGGCTTTCTCCAGGTATTGATGAACTAGTTAAAAAGCTAAAAGCTAACAACATTGATGTATATCTGATTTCGGGAGGCTTTCGTCAAATGATCAATCCTGTTGCATCAATTCTTGGGATTCCACATGAGAATATTTTTGCCAACCAATTGCTTTTTGGAAGCAATGGTGAATTTATGGGGTTTGACAAGAATGAGCCTACTTCAAGGAGTGGGGGAAAAGCAATTGCAGTACAACAATTGAGGAAGGCTCGTGGATACAAGACATTGGTCATGACTGGGGACGGTGCAACTGATCTTGAGGCCTGTAAACCAGGAGGCGCTGACTTGTTTATATGCTATGCTGGCGTTCAACTTCGAGAAAATGTCGCTGCAAAAGCTGACTGGCTGGTTTTCAGTTTTGTAGATATAATCAGCTCCCTTGACTAA

Protein sequence

MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPIDKLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD
Homology
BLAST of Bhi02G000066 vs. TAIR 10
Match: AT1G18640.2 (3-phosphoserine phosphatase )

HSP 1 Score: 409.8 bits (1052), Expect = 1.8e-114
Identity = 208/295 (70.51%), Postives = 238/295 (80.68%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQP-- 60
           ME+L ++R+ P+   CRK+ SLF     L+  R   +  + +        P  ASVQP  
Sbjct: 1   MEALTTSRVVPVQVPCRKLSSLFANFSCLELRRYPCRGLVSIMNHPKLLRPVTASVQPHE 60

Query: 61  IDKLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
           +  L    N  PSKE+L+LWR   AVCFDVDSTVCVDEGIDELA+FCG+G+AVAEWTARA
Sbjct: 61  LSTLGHEGNIVPSKEILDLWRSVEAVCFDVDSTVCVDEGIDELAEFCGAGKAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE+ALAARLSLF PSLS+VEE+L KRPPRLSPGI+ELVKKL+ANNIDVYLISGG
Sbjct: 121 MGGSVPFEEALAARLSLFKPSLSKVEEYLDKRPPRLSPGIEELVKKLRANNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIP ENIFAN LLFG++GEF+GFD+NEPTSRSGGKA AVQQ+RK R Y
Sbjct: 181 FRQMINPVASILGIPRENIFANNLLFGNSGEFLGFDENEPTSRSGGKAKAVQQIRKGRLY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KT+ M GDGATDLEA KPGGADLFICYAGVQLRE VAA ADWL+F F  +I+SLD
Sbjct: 241 KTMAMIGDGATDLEARKPGGADLFICYAGVQLREAVAANADWLIFKFESLINSLD 295

BLAST of Bhi02G000066 vs. ExPASy Swiss-Prot
Match: O82796 (Phosphoserine phosphatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSP PE=1 SV=2)

HSP 1 Score: 409.8 bits (1052), Expect = 2.5e-113
Identity = 208/295 (70.51%), Postives = 238/295 (80.68%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQP-- 60
           ME+L ++R+ P+   CRK+ SLF     L+  R   +  + +        P  ASVQP  
Sbjct: 1   MEALTTSRVVPVQVPCRKLSSLFANFSCLELRRYPCRGLVSIMNHPKLLRPVTASVQPHE 60

Query: 61  IDKLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
           +  L    N  PSKE+L+LWR   AVCFDVDSTVCVDEGIDELA+FCG+G+AVAEWTARA
Sbjct: 61  LSTLGHEGNIVPSKEILDLWRSVEAVCFDVDSTVCVDEGIDELAEFCGAGKAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE+ALAARLSLF PSLS+VEE+L KRPPRLSPGI+ELVKKL+ANNIDVYLISGG
Sbjct: 121 MGGSVPFEEALAARLSLFKPSLSKVEEYLDKRPPRLSPGIEELVKKLRANNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIP ENIFAN LLFG++GEF+GFD+NEPTSRSGGKA AVQQ+RK R Y
Sbjct: 181 FRQMINPVASILGIPRENIFANNLLFGNSGEFLGFDENEPTSRSGGKAKAVQQIRKGRLY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KT+ M GDGATDLEA KPGGADLFICYAGVQLRE VAA ADWL+F F  +I+SLD
Sbjct: 241 KTMAMIGDGATDLEARKPGGADLFICYAGVQLREAVAANADWLIFKFESLINSLD 295

BLAST of Bhi02G000066 vs. ExPASy Swiss-Prot
Match: Q2KHU0 (Phosphoserine phosphatase OS=Bos taurus OX=9913 GN=PSPH PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.4e-60
Identity = 114/221 (51.58%), Postives = 156/221 (70.59%), Query Frame = 0

Query: 73  EVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMGGSVPFEDALAAR 132
           E+  L+  A+AVCFDVDSTV  +EGIDELA FCG  +AV+E T +AMGG+VPF+ AL  R
Sbjct: 6   ELRNLFCSADAVCFDVDSTVIQEEGIDELAKFCGVEDAVSEMTRQAMGGAVPFKAALTQR 65

Query: 133 LSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFRQMINPVASILGI 192
           L+L  PS  QV+  LA+ PP L+PGI ELV +L+  N+ V+LISGGFR ++  VAS L I
Sbjct: 66  LALIQPSREQVQRLLAEHPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNI 125

Query: 193 PHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKTLVMTGDGATDLE 252
           P  N+FAN+L F  NGE+ GFD+ +PT+ SGGK   ++ L++   +K +VM GDGATD+E
Sbjct: 126 PSTNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIVMVGDGATDME 185

Query: 253 ACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           AC P  AD FI + G  +R+ V   A+W +  FV+++ +L+
Sbjct: 186 ACPP--ADAFIGFGGNVIRQQVKDNAEWYITDFVELLGALE 224

BLAST of Bhi02G000066 vs. ExPASy Swiss-Prot
Match: Q99LS3 (Phosphoserine phosphatase OS=Mus musculus OX=10090 GN=Psph PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.1e-59
Identity = 113/221 (51.13%), Postives = 155/221 (70.14%), Query Frame = 0

Query: 73  EVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMGGSVPFEDALAAR 132
           E+ +L+  A+AVCFDVDSTV  +EGIDELA FCG   AV+E T RAMGG++PF+DAL  R
Sbjct: 6   ELRKLFCSADAVCFDVDSTVIREEGIDELAKFCGVEAAVSEMTRRAMGGALPFKDALTQR 65

Query: 133 LSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFRQMINPVASILGI 192
           L+L  PS  QV+  LA+ PP L+PGI ELV +L+  N+ V+LISGGFR ++  VA+ L I
Sbjct: 66  LALIQPSRDQVQRLLAEHPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVAAKLNI 125

Query: 193 PHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKTLVMTGDGATDLE 252
           P  N+FAN+L F  NGE+ GFD+ +PT+ SGGK   ++ L++   +K ++M GDGATD+E
Sbjct: 126 PTTNVFANRLKFYFNGEYAGFDEMQPTAESGGKGKVIRFLKEKFHFKKIIMIGDGATDME 185

Query: 253 ACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           AC P  AD FI + G  +R+ V   A W +  FV+++  L+
Sbjct: 186 ACPP--ADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELE 224

BLAST of Bhi02G000066 vs. ExPASy Swiss-Prot
Match: Q5M819 (Phosphoserine phosphatase OS=Rattus norvegicus OX=10116 GN=Psph PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.0e-59
Identity = 113/221 (51.13%), Postives = 154/221 (69.68%), Query Frame = 0

Query: 73  EVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMGGSVPFEDALAAR 132
           E+ +L+  A+AVCFDVDSTV  +EGIDELA FCG   AV+E T RAMGG++PF+DAL  R
Sbjct: 6   ELRKLFCSADAVCFDVDSTVIREEGIDELAKFCGVEAAVSEMTRRAMGGALPFKDALTER 65

Query: 133 LSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFRQMINPVASILGI 192
           L+L  PS  QV+  LA+ PP L+PGI ELV +L+  N+ V+LISGGFR ++  VA+ L I
Sbjct: 66  LALIQPSRDQVQRLLAEHPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVAAKLNI 125

Query: 193 PHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKTLVMTGDGATDLE 252
           P  N+FAN+L F  NGE+ GFD+ +PT+ SGGK   +  L++   +K ++M GDGATD+E
Sbjct: 126 PTTNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIGFLKEKFHFKKIIMIGDGATDME 185

Query: 253 ACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           AC P  AD FI + G  +R+ V   A W +  FV+++  L+
Sbjct: 186 ACPP--ADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELE 224

BLAST of Bhi02G000066 vs. ExPASy Swiss-Prot
Match: P78330 (Phosphoserine phosphatase OS=Homo sapiens OX=9606 GN=PSPH PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.0e-58
Identity = 112/221 (50.68%), Postives = 155/221 (70.14%), Query Frame = 0

Query: 73  EVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMGGSVPFEDALAAR 132
           E+ +L+  A+AVCFDVDSTV  +EGIDELA  CG  +AV+E T RAMGG+VPF+ AL  R
Sbjct: 6   ELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTER 65

Query: 133 LSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFRQMINPVASILGI 192
           L+L  PS  QV+  +A++PP L+PGI ELV +L+  N+ V+LISGGFR ++  VAS L I
Sbjct: 66  LALIQPSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNI 125

Query: 193 PHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKTLVMTGDGATDLE 252
           P  N+FAN+L F  NGE+ GFD+ +PT+ SGGK   ++ L++   +K ++M GDGATD+E
Sbjct: 126 PATNVFANRLKFYFNGEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDME 185

Query: 253 ACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           AC P  AD FI + G  +R+ V   A W +  FV+++  L+
Sbjct: 186 ACPP--ADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELE 224

BLAST of Bhi02G000066 vs. NCBI nr
Match: XP_038878378.1 (phosphoserine phosphatase, chloroplastic [Benincasa hispida] >XP_038878379.1 phosphoserine phosphatase, chloroplastic [Benincasa hispida])

HSP 1 Score: 592.4 bits (1526), Expect = 2.1e-165
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID
Sbjct: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60

Query: 61  KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMG 120
           KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMG
Sbjct: 61  KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARAMG 120

Query: 121 GSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFR 180
           GSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFR
Sbjct: 121 GSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGGFR 180

Query: 181 QMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKT 240
           QMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKT
Sbjct: 181 QMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGYKT 240

Query: 241 LVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           LVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD
Sbjct: 241 LVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 293

BLAST of Bhi02G000066 vs. NCBI nr
Match: XP_008453274.1 (PREDICTED: phosphoserine phosphatase, chloroplastic [Cucumis melo] >XP_008453275.1 PREDICTED: phosphoserine phosphatase, chloroplastic [Cucumis melo])

HSP 1 Score: 541.6 bits (1394), Expect = 4.2e-150
Identity = 271/295 (91.86%), Postives = 282/295 (95.59%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLSARIKPLPTHCR+IHS FRPKLFLQF+RRLDK  ME G+C  SFNP AASVQPI+
Sbjct: 1   MESLLSARIKPLPTHCRQIHSSFRPKLFLQFARRLDKGGMETGRCGMSFNPIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LS+FNNTSPSKEVLELWR ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GPTLSKFNNTSPSKEVLELWRSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKAN+IDVYLISGG
Sbjct: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANSIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKA+AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAVAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQLRE VA+KADWLVF+FVD+ISSLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQLREKVASKADWLVFNFVDMISSLD 295

BLAST of Bhi02G000066 vs. NCBI nr
Match: XP_004138197.1 (phosphoserine phosphatase, chloroplastic [Cucumis sativus] >KGN63727.1 hypothetical protein Csa_013316 [Cucumis sativus])

HSP 1 Score: 538.5 bits (1386), Expect = 3.6e-149
Identity = 268/295 (90.85%), Postives = 282/295 (95.59%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLSARIKPLPTHCR+IHS FRPKLFLQF+RRLDK  MEMG+C  SFNP AASVQPI+
Sbjct: 1   MESLLSARIKPLPTHCRQIHSSFRPKLFLQFARRLDKGGMEMGRCGMSFNPIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LS+FNNTSPSKE LELWR ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GPTLSKFNNTSPSKEALELWRSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFEDALAARLSLFNPSLSQVEEFLAK+PPRLSPGIDELVKKLKAN+IDVYLISGG
Sbjct: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKKPPRLSPGIDELVKKLKANSIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGSNGEF+GFDK+EPTSRSGGKA+AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFVGFDKSEPTSRSGGKAVAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICY GVQLRENVA+KADWLVF+FVD+ISSLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYGGVQLRENVASKADWLVFNFVDMISSLD 295

BLAST of Bhi02G000066 vs. NCBI nr
Match: XP_022971797.1 (phosphoserine phosphatase, chloroplastic [Cucurbita maxima] >XP_022971805.1 phosphoserine phosphatase, chloroplastic [Cucurbita maxima] >XP_022971811.1 phosphoserine phosphatase, chloroplastic [Cucurbita maxima] >XP_022971821.1 phosphoserine phosphatase, chloroplastic [Cucurbita maxima] >XP_022971828.1 phosphoserine phosphatase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 528.1 bits (1359), Expect = 4.9e-146
Identity = 264/295 (89.49%), Postives = 277/295 (93.90%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           M+SLLSARIKPLPTHCR+IHS  RP L LQFSRRL K  MEMG+CRTSFN  AASVQP++
Sbjct: 1   MDSLLSARIKPLPTHCRQIHSTIRPTLLLQFSRRLGKSQMEMGRCRTSFNSIAASVQPVE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LSQFNNT PSKEVLELW+ ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GSALSQFNNTLPSKEVLELWKSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE ALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKA+NIDVYLISGG
Sbjct: 121 MGGSVPFEVALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKASNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGS GEF+GFD+NEPTSRSGGKA AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSKGEFIGFDENEPTSRSGGKATAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQLRENVAAKADWLVF+FVD+I+SLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQLRENVAAKADWLVFNFVDLINSLD 295

BLAST of Bhi02G000066 vs. NCBI nr
Match: XP_023530852.1 (phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530853.1 phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530854.1 phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530855.1 phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530856.1 phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530857.1 phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.0 bits (1351), Expect = 4.1e-145
Identity = 263/295 (89.15%), Postives = 276/295 (93.56%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           M+SLLSARIKPLPTHCR+IHS  RP L LQFSRRL K  MEMG+CRTSFN  AASVQPI+
Sbjct: 1   MDSLLSARIKPLPTHCRQIHSTIRPTLLLQFSRRLGKSQMEMGRCRTSFNSIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LSQFNNT PSKEVLELW+ ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GSALSQFNNTLPSKEVLELWKSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE ALAARLSLFNPSLSQ+EEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG
Sbjct: 121 MGGSVPFEVALAARLSLFNPSLSQLEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGS GEF+GFD+NEPTSRSGGKA AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSKGEFIGFDENEPTSRSGGKATAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQ RENVAAKADWLVF+FVD+I+SL+
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQHRENVAAKADWLVFNFVDLINSLE 295

BLAST of Bhi02G000066 vs. ExPASy TrEMBL
Match: A0A1S3BVV9 (O-phosphoserine phosphohydrolase OS=Cucumis melo OX=3656 GN=LOC103494043 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.1e-150
Identity = 271/295 (91.86%), Postives = 282/295 (95.59%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLSARIKPLPTHCR+IHS FRPKLFLQF+RRLDK  ME G+C  SFNP AASVQPI+
Sbjct: 1   MESLLSARIKPLPTHCRQIHSSFRPKLFLQFARRLDKGGMETGRCGMSFNPIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LS+FNNTSPSKEVLELWR ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GPTLSKFNNTSPSKEVLELWRSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKAN+IDVYLISGG
Sbjct: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANSIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKA+AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAVAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQLRE VA+KADWLVF+FVD+ISSLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQLREKVASKADWLVFNFVDMISSLD 295

BLAST of Bhi02G000066 vs. ExPASy TrEMBL
Match: A0A0A0LSF8 (O-phosphoserine phosphohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G013750 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.7e-149
Identity = 268/295 (90.85%), Postives = 282/295 (95.59%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLSARIKPLPTHCR+IHS FRPKLFLQF+RRLDK  MEMG+C  SFNP AASVQPI+
Sbjct: 1   MESLLSARIKPLPTHCRQIHSSFRPKLFLQFARRLDKGGMEMGRCGMSFNPIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LS+FNNTSPSKE LELWR ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GPTLSKFNNTSPSKEALELWRSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFEDALAARLSLFNPSLSQVEEFLAK+PPRLSPGIDELVKKLKAN+IDVYLISGG
Sbjct: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKKPPRLSPGIDELVKKLKANSIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGSNGEF+GFDK+EPTSRSGGKA+AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFVGFDKSEPTSRSGGKAVAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICY GVQLRENVA+KADWLVF+FVD+ISSLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYGGVQLRENVASKADWLVFNFVDMISSLD 295

BLAST of Bhi02G000066 vs. ExPASy TrEMBL
Match: A0A6J1I484 (O-phosphoserine phosphohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470473 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.4e-146
Identity = 264/295 (89.49%), Postives = 277/295 (93.90%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           M+SLLSARIKPLPTHCR+IHS  RP L LQFSRRL K  MEMG+CRTSFN  AASVQP++
Sbjct: 1   MDSLLSARIKPLPTHCRQIHSTIRPTLLLQFSRRLGKSQMEMGRCRTSFNSIAASVQPVE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LSQFNNT PSKEVLELW+ ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GSALSQFNNTLPSKEVLELWKSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE ALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKA+NIDVYLISGG
Sbjct: 121 MGGSVPFEVALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKASNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGS GEF+GFD+NEPTSRSGGKA AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSKGEFIGFDENEPTSRSGGKATAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQLRENVAAKADWLVF+FVD+I+SLD
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQLRENVAAKADWLVFNFVDLINSLD 295

BLAST of Bhi02G000066 vs. ExPASy TrEMBL
Match: A0A6J1F5C0 (O-phosphoserine phosphohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111440993 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.0e-145
Identity = 264/295 (89.49%), Postives = 276/295 (93.56%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           M+SLLSARIKPLPTH R+IHS  RP L LQFSRRL K  MEMG+CRTSFN  AASVQPI+
Sbjct: 1   MDSLLSARIKPLPTHSRQIHSTIRPTLLLQFSRRLGKSQMEMGRCRTSFNSIAASVQPIE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LSQFNNT PSKEVLELW+ ANAVCFDVDSTVCVDEGIDELADFCG+GEAVAEWTARA
Sbjct: 61  GSALSQFNNTLPSKEVLELWKSANAVCFDVDSTVCVDEGIDELADFCGAGEAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFE ALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG
Sbjct: 121 MGGSVPFEVALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGS GEF+GFD+NEPTSRSGGKA AVQQLRKARGY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSKGEFIGFDENEPTSRSGGKATAVQQLRKARGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICYAGVQLRENVAAKADWLVF+FVD+I+SL+
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYAGVQLRENVAAKADWLVFNFVDLINSLE 295

BLAST of Bhi02G000066 vs. ExPASy TrEMBL
Match: A0A6J1BZR2 (O-phosphoserine phosphohydrolase OS=Momordica charantia OX=3673 GN=LOC111006963 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.9e-143
Identity = 258/295 (87.46%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 1   MESLLSARIKPLPTHCRKIHSLFRPKLFLQFSRRLDKCHMEMGKCRTSFNPFAASVQPID 60
           MESLLS+RI PLPTHCR+I S  RP LFLQFSRR  +  MEMG+CR SFNP AASVQP++
Sbjct: 1   MESLLSSRITPLPTHCRQIQSSLRPSLFLQFSRRSVEGQMEMGRCRMSFNPIAASVQPLE 60

Query: 61  --KLSQFNNTSPSKEVLELWRGANAVCFDVDSTVCVDEGIDELADFCGSGEAVAEWTARA 120
              LS FNNTSPSKEVLELWR ANAVCFDVDSTVCVDEGIDELADFCG+G+AVAEWTARA
Sbjct: 61  GPTLSHFNNTSPSKEVLELWRSANAVCFDVDSTVCVDEGIDELADFCGAGKAVAEWTARA 120

Query: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKANNIDVYLISGG 180
           MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKA NIDVYLISGG
Sbjct: 121 MGGSVPFEDALAARLSLFNPSLSQVEEFLAKRPPRLSPGIDELVKKLKAKNIDVYLISGG 180

Query: 181 FRQMINPVASILGIPHENIFANQLLFGSNGEFMGFDKNEPTSRSGGKAIAVQQLRKARGY 240
           FRQMINPVASILGIPHENIFANQLLFGS+GEF+GFDKNEPTSRSGGKA AVQQLRKA GY
Sbjct: 181 FRQMINPVASILGIPHENIFANQLLFGSSGEFLGFDKNEPTSRSGGKATAVQQLRKAHGY 240

Query: 241 KTLVMTGDGATDLEACKPGGADLFICYAGVQLRENVAAKADWLVFSFVDIISSLD 294
           KTLVMTGDGATDLEA KPGGADLFICY G+QLRENVAAKADWLVF+F+D+I+SL+
Sbjct: 241 KTLVMTGDGATDLEARKPGGADLFICYGGIQLRENVAAKADWLVFNFLDMINSLE 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G18640.21.8e-11470.513-phosphoserine phosphatase [more]
Match NameE-valueIdentityDescription
O827962.5e-11370.51Phosphoserine phosphatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSP ... [more]
Q2KHU09.4e-6051.58Phosphoserine phosphatase OS=Bos taurus OX=9913 GN=PSPH PE=2 SV=1[more]
Q99LS36.1e-5951.13Phosphoserine phosphatase OS=Mus musculus OX=10090 GN=Psph PE=1 SV=1[more]
Q5M8198.0e-5951.13Phosphoserine phosphatase OS=Rattus norvegicus OX=10116 GN=Psph PE=2 SV=1[more]
P783301.0e-5850.68Phosphoserine phosphatase OS=Homo sapiens OX=9606 GN=PSPH PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_038878378.12.1e-165100.00phosphoserine phosphatase, chloroplastic [Benincasa hispida] >XP_038878379.1 pho... [more]
XP_008453274.14.2e-15091.86PREDICTED: phosphoserine phosphatase, chloroplastic [Cucumis melo] >XP_008453275... [more]
XP_004138197.13.6e-14990.85phosphoserine phosphatase, chloroplastic [Cucumis sativus] >KGN63727.1 hypotheti... [more]
XP_022971797.14.9e-14689.49phosphoserine phosphatase, chloroplastic [Cucurbita maxima] >XP_022971805.1 phos... [more]
XP_023530852.14.1e-14589.15phosphoserine phosphatase, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023530... [more]
Match NameE-valueIdentityDescription
A0A1S3BVV92.1e-15091.86O-phosphoserine phosphohydrolase OS=Cucumis melo OX=3656 GN=LOC103494043 PE=3 SV... [more]
A0A0A0LSF81.7e-14990.85O-phosphoserine phosphohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G013750 PE=3... [more]
A0A6J1I4842.4e-14689.49O-phosphoserine phosphohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470473 PE=... [more]
A0A6J1F5C02.0e-14589.49O-phosphoserine phosphohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111440993 P... [more]
A0A6J1BZR21.9e-14387.46O-phosphoserine phosphohydrolase OS=Momordica charantia OX=3673 GN=LOC111006963 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 140..292
e-value: 8.3E-54
score: 183.6
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 69..123
e-value: 9.0E-6
score: 27.5
IPR004469Phosphoserine phosphataseTIGRFAMTIGR00338TIGR00338coord: 76..277
e-value: 1.7E-45
score: 153.3
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 82..256
e-value: 7.5E-17
score: 62.3
NoneNo IPR availableTIGRFAMTIGR01488TIGR01488coord: 83..255
e-value: 3.4E-29
score: 100.0
NoneNo IPR availablePANTHERPTHR43344:SF16BNAA06G12800D PROTEINcoord: 1..293
NoneNo IPR availablePANTHERPTHR43344PHOSPHOSERINE PHOSPHATASEcoord: 1..293
NoneNo IPR availableCDDcd04309HAD_PSP_eucoord: 83..285
e-value: 4.13563E-119
score: 338.103
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 72..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M000066Bhi02M000066mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0006564 L-serine biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0036424 L-phosphoserine phosphatase activity
molecular_function GO:0000287 magnesium ion binding