Bhi02G000026 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000026
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	protein MIS12 homolog
Location	chr2: 810246 .. 813820 (+)
RNA-Seq Expression	Bhi02G000026
Synteny	Bhi02G000026

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTATTAGCATTAGCGCCACTGTGTAAGCCGGCTCCGTGTCCCCATCATTCGCCACTACCATTTTGGCGGCAAAATTTCTCCATTTATGCGAAAGCTTTGGAGCTTCCATTTTGAATTTCTCGTCGTCTTCTTCATTGGAAAAGAAGCTTTTTCAGGAAGCGATAGAGTAATTCTGATGGAAGGAAGTAAAGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTAGACGATTTAGTTGATGACGCTTTCGGTTTCTATCAATCGTATTGCTCTCTCTGTTTCCGTCTCTTCAACTTAGGTTTTCAATTTTGTTCGGTTGATTTTATTGATTCTTATCCATTCTTTTTCCCTTCAGACAAGCATCAGCGACCTTGAAGACCGAGGGCTCGGATCGATCCCAGGATCTTACCCTGGTAAGGTTTAATTTGCATTTTATTACTTGACATTTTCTTATCAGCCGTTAATTTCCGGCTTTTTGAGTTTGTACTCTTGAGCACTTCTTTGAATTGTTTCGGTTTTCTTTGAACAAAATTGTTTAATTGCCTCGTTCCTGTAGGGAATATCGCGAGTCCGAGCGTTGGTTCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTCTCAGTACCTGAAGGTTTCTCTTTACCATCAAATGTAGGTTTTTCTTCTACTCTGTCTGTGATGAGGCTCCGATGCCCAAGGACTAAAATATTGAAGTTGGTATCAATATCGAAATTTCAAGTTATAGTCTATCAATTGAATATCACTATCGATAGATATTTGTGTAAATCAATAAATTTATTAAAAAAAAATATTTAAATTAATAATTAAGTTGTTTTGGCTTTCAAATAAGTTATAGCTCTTATTGAGATTCATAGATAACATCTTCTTATGTTTTTATGTGTTTATAAAAGATACCGGAGATGTCTATAAGTCCTTAATATCAATGTCAAATCTTCCTATTCATAGATATATCAACATATCAACAAATAATCACAACCTTGTCTGTGCTTTGCTATGTAAAGTTGCTCTATTAGTGTTGTTTTTGTAACTGAATTACTCGCCCTTTATGCCCAAATCTTTTCACTAGTGATTAATAATTGAGCATGTAAAATGGCTGCTTGTTGGTTCTGCGAGTTTCCCCCTTGTGTACTGGCTAGAGAGCTTCCTCAACTTTTGGTTCGAGGGTGAACAAATTTTGAGATATTGGAGAAATGTGCATCATCAAGTTTCAAGTTTTTTTACGAGATATGTTCATTGTTTTAGATGCAGTTTCGAGTTTCCATATGTTTTACCTCATTGATTCCTTTTCTTGTTTGCAAGTCAATGTGGTTCCACTGTCACTAGAAATATCGCCGTGATTGTAAAAGATTCTTTTGCTCTGAAGGAATGTAAGCAAATGTCTGCAGCATTTCTTAGAAAATCTCTGTCATGGTGCATCGTGGCTTTTCTTTTTAAATTATTTTTGAAGTCTAACCTATGTGAGGTTCAGTTCCACTCCTTTCATTTTGCACCAATATAGATACTTGGGGTTGGGAATTTCTGTCTGTCTCACTGGCATCTTAATGGAATCTGCGATATCTTATTGTCATGTTGCTAAATTATCAATGGTTCCCATATCTTGTTTTAGAAATTTGAGTTATGGTTAATTTAGTCTATGTATTATCTTACTTACTACTTTTTCTTCTCTCTAACGCAATAAACATATTTCTGATTCAGACCTAAATATTTTCTTTAAGTTGTCTTAGGATGATTCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGGTCTGGATACAGAGCTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTTTGTTTATTACGTCTTGTGTTTATGTACATCATATTTTATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAATATTGTATTGAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTACCAGTAACTCCCAAATCAATCATTTCAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAAATGTTCCAGGGTAATTAAGTTGTACTTAAAATCAATTTTTTCCTTTCTGCACTCCTGGTTTTACATGCGAATTACTTTACTGGTATACTCATGAAGGCATACTCAAAATCATGTATGAACCTGATTATCGTCGTAGTCAGCCTGGTAGATTAAAGTTTCTGTAAACACTGACCTTCTCCTTCAATGGATTGTGCTGGCCTGGATATCTCATGGTGACACAAACTCTAAATTCTAATAGTTTCAAATGATATTTTAGTCAACACCTGCAAAATCATTTCATTTGGGCAAATTTTATATTACCTCTAGGAATAGGGTTAGTGAATTTCTCTTTTTGTTTCCCATTTTCTGTCGTTCATCCATCAGAGAGGAAATTCAGTCCGTGAATAAGGCAATTTCTCTTGCTATTTTCATTTTGAAGGCCACTTGGACAAAATTTATTCCTTAGCATTAAATTTCGTTGTACTGAAAACTTTAACAGTAGGTAGGTAAAAGCTGTTCATTATTTTAGGAAGCAGACTTCTAACATGTATCAATTTCTCGCAGAAATGATGGGAACTGCATCGGAGCTGCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAATCCAAGCTGACTAAAGTAGAGAAGGTTCATACAAACGGGGACATCTCTCACTATCATAATGGTATGATTATGAAGCTACTTCATTATCAATCAAGCTGGTTTTAGCTTTCTTGAAGCTGAATATCATCTCACATGCTGGTTGACGCTTGTTTCTGTTCTGCAGGTTTATCTAATGCTAAGCTGGATGACATTAAAATTTTTTTAGATGATTTGAAGACCCTTTGAAGTATTTCATTATGACATCAACCTTACTGTATATGGTGCTTGCTGTGAAACTTGTCACTACGAGTTCTTCAATGTTTAAACTTGATTTAGCTGTTGGGAGATATTCTAAACCAAATTTGTATAATATTTCGTTTGGATTGATGACTATCCTTAGCTCTCTCCTTAAGAGTTTGCAGATGGGCCATGAGTAGGTGTTGGCTCAATCCTATAAATTATTATGGTTCGTTTCAGAAGCTTCTCTTTTGTTTTATTACTACTCTTGATGTGGAAATCCTATCAAATCTCAAATGGAAATTTAGACTTAGAATTGTGTGGTAGTGACTTGGGACCGGAAATTTAAAGTGCAACCCGTTTTAGATCGTCATTGCACCTTTTGAAACGTTTCTGAAATGTGAAGAATAATATTGCAATTTTTTAAATAAATGGAAAACCCTCTCATAAGTGTTTTTTAAGTTTTGGGGTTTTAGAATGTTTGTTTTGCGATGTTGGCACTAAATGCAATTGATTTTTTTAGGCAGACATAAACAAACAACAAAGTCACCACAACCAAACGTAACAAAAGCCCTACATGAGGAGCGGCGGAACACAATCTGAAGAGAAAAAAACATCTTCTCCTAGACAAAGCATCCTTGAAAGGAG

mRNA sequence

GACTATTAGCATTAGCGCCACTGTGTAAGCCGGCTCCGTGTCCCCATCATTCGCCACTACCATTTTGGCGGCAAAATTTCTCCATTTATGCGAAAGCTTTGGAGCTTCCATTTTGAATTTCTCGTCGTCTTCTTCATTGGAAAAGAAGCTTTTTCAGGAAGCGATAGAGTAATTCTGATGGAAGGAAGTAAAGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTAGACGATTTAGTTGATGACGCTTTCGGTTTCTATCAATCACAAGCATCAGCGACCTTGAAGACCGAGGGCTCGGATCGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTTCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTCTCAGTACCTGAAGGTTTCTCTTTACCATCAAATGATGATTCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGGTCTGGATACAGAGCTAGATTTTTTGAGGAATAAGCTCTCTGAGGTCAGAAAAGAGAATATTGTATTGAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTACCAGTAACTCCCAAATCAATCATTTCAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAAATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTGCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAATCCAAGCTGACTAAAGTAGAGAAGGTTCATACAAACGGGGACATCTCTCACTATCATAATGGTTTATCTAATGCTAAGCTGGATGACATTAAAATTTTTTTAGATGATTTGAAGACCCTTTGAAGTATTTCATTATGACATCAACCTTACTGTATATGGTGCTTGCTGTGAAACTTGTCACTACGAGTTCTTCAATGTTTAAACTTGATTTAGCTGTTGGGAGATATTCTAAACCAAATTTGTATAATATTTCGTTTGGATTGATGACTATCCTTAGCTCTCTCCTTAAGAGTTTGCAGATGGGCCATGAGTAGGTGTTGGCTCAATCCTATAAATTATTATGGTTCGTTTCAGAAGCTTCTCTTTTGTTTTATTACTACTCTTGATGTGGAAATCCTATCAAATCTCAAATGGAAATTTAGACTTAGAATTGTGTGGTAGTGACTTGGGACCGGAAATTTAAAGTGCAACCCGTTTTAGATCGTCATTGCACCTTTTGAAACGTTTCTGAAATGTGAAGAATAATATTGCAATTTTTTAAATAAATGGAAAACCCTCTCATAAGTGTTTTTTAAGTTTTGGGGTTTTAGAATGTTTGTTTTGCGATGTTGGCACTAAATGCAATTGATTTTTTTAGGCAGACATAAACAAACAACAAAGTCACCACAACCAAACGTAACAAAAGCCCTACATGAGGAGCGGCGGAACACAATCTGAAGAGAAAAAAACATCTTCTCCTAGACAAAGCATCCTTGAAAGGAG

Coding sequence (CDS)

ATGCGAAAGCTTTGGAGCTTCCATTTTGAATTTCTCGTCGTCTTCTTCATTGGAAAAGAAGCTTTTTCAGGAAGCGATAGAGTAATTCTGATGGAAGGAAGTAAAGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTAGACGATTTAGTTGATGACGCTTTCGGTTTCTATCAATCACAAGCATCAGCGACCTTGAAGACCGAGGGCTCGGATCGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTTCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTCTCAGTACCTGAAGGTTTCTCTTTACCATCAAATGATGATTCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGGTCTGGATACAGAGCTAGATTTTTTGAGGAATAAGCTCTCTGAGGTCAGAAAAGAGAATATTGTATTGAACCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTACCAGTAACTCCCAAATCAATCATTTCAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAAATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTGCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAATCCAAGCTGACTAAAGTAGAGAAGGTTCATACAAACGGGGACATCTCTCACTATCATAATGGTTTATCTAATGCTAAGCTGGATGACATTAAAATTTTTTTAGATGATTTGAAGACCCTTTGA

Protein sequence

MRKLWSFHFEFLVVFFIGKEAFSGSDRVILMEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL

Homology

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BLAST of Bhi02G000026 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 207.2 bits (526), Expect = 1.6e-53
Identity = 116/243 (47.74%), Postives = 168/243 (69.14%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSD--RSQDL 90
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AF FY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 91  TLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVG 150
           + GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP +++S   +S+  D  YD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 151 LDTELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQ 210
           LD ELD LR+KL+ V K ++ L+ ELQALER + +    +   NEAL+LY+++S++E+F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 211 EMMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDL 270
           EM   ASELRA + +LK RRM+ S+  KV+++  +G    +     + KL+D++ F  +L
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKRLKNHG--KEFSAMTFDGKLEDLEKFQAEL 238

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BLAST of Bhi02G000026 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.3e-52
Identity = 116/243 (47.74%), Postives = 168/243 (69.14%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSD--RSQDL 90
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AF FY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 91  TLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVG 150
           + GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP +++S   +S+  D  YD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 151 LDTELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQ 210
           LD ELD LR+KL+ V K ++ L+ ELQALER + +    +   NEAL+LY+++S++E+F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 211 EMMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDL 270
           EM   ASELRA + +LK RRM+ S+  KV+++  +G    +     + KL+D++ F  +L
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKRLKNHG--KEFSAMTFDGKLEDLEKFQAEL 238

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BLAST of Bhi02G000026 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 525.4 bits (1352), Expect = 2.9e-145
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MRKLWSFHFEFLVVFFIGKEAFSGSDRVILMEGSKGEAVFDSLNLNPQLFINEALNIVDD 60
           MRKLWSFHFEFLVVFFIGKEAFSGSDRVILMEGSKGEAVFDSLNLNPQLFINEALNIVDD
Sbjct: 1   MRKLWSFHFEFLVVFFIGKEAFSGSDRVILMEGSKGEAVFDSLNLNPQLFINEALNIVDD 60

Query: 61  LVDDAFGFYQSQASATLKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKYCLNHCFS 120
           LVDDAFGFYQSQASATLKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKYCLNHCFS
Sbjct: 61  LVDDAFGFYQSQASATLKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKYCLNHCFS 120

Query: 121 VPEGFSLPSNDDSPGVTSISHDYDVGLDTELDFLRNKLSEVRKENIVLNQELQALERQTA 180
           VPEGFSLPSNDDSPGVTSISHDYDVGLDTELDFLRNKLSEVRKENIVLNQELQALERQTA
Sbjct: 121 VPEGFSLPSNDDSPGVTSISHDYDVGLDTELDFLRNKLSEVRKENIVLNQELQALERQTA 180

Query: 181 TSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKVHT 240
           TSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKVHT
Sbjct: 181 TSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKVHT 240

Query: 241 NGDISHYHNGLSNAKLDDIKIFLDDLKTL 270
           NGDISHYHNGLSNAKLDDIKIFLDDLKTL
Sbjct: 241 NGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

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BLAST of Bhi02G000026 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.2 bits (1071), Expect = 1.1e-112
Identity = 215/241 (89.21%), Postives = 226/241 (93.78%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAF FYQ+QASA+LKTEGSDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVGLD 150
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SP VTS+S D  YDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 151 TELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEM 210
           TELDFLRNKLSEVRKEN+VLNQELQALERQTA+SNSQ+N FNEALQLYEQNSVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 211 MGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKT 270
           MGTASELRAKIGKLKKRRMEESKLTKVEK+HTNGDISH+H G SNAKLDDI+ FL DLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKT 240

[Close]

BLAST of Bhi02G000026 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 414.8 bits (1065), Expect = 5.5e-112
Identity = 211/239 (88.28%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASATLKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSI+HD DV LDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 270
           TASELR+KIGK+KKRR EE+KL KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLK +
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Bhi02G000026 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414.5 bits (1064), Expect = 7.2e-112
Identity = 214/241 (88.80%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAF FYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVGLD 150
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SP VTS+S D  YDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 151 TELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEM 210
           TELDFLRNKLSEVRKEN+VLNQELQALERQTA+SNSQ+N FNEALQLYEQNSVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 211 MGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKT 270
           MGTASELRAKIGKLKKRRMEESKLTKVEK+HTNGDISH+H G SNAKLDDI+ FL DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKA 240

[Close]

BLAST of Bhi02G000026 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 414.1 bits (1063), Expect = 9.4e-112
Identity = 211/239 (88.28%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSI+HD DV LDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 270
           TASELRAKIGK+KKRR EE+KL KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLK +
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.7e-112
Identity = 211/239 (88.28%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASATLKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSI+HD DV LDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 270
           TASELR+KIGK+KKRR EE+KL KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLK +
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.5e-112
Identity = 211/239 (88.28%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSI+HD DV LDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210
           LD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ 
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 270
           TASELRAKIGK+KKRR EE+KL KVEKVHTNGDISH+HNG SNAKLDDI+ FL DLK +
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 213/241 (88.38%), Postives = 222/241 (92.12%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAF FYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVGLD 150
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SP VTS+S D  YDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 151 TELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEM 210
           TELDFLR  LSEVRKENIVLNQELQALERQTA+SNSQ+N FNEALQLYEQNSVNEMFQEM
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 211 MGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKT 270
           MGTASELRAKIGKLKKRRMEESKLTKVEK+HTN DISH+H G SNAKLDDI+ FL DLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLKT 240

[Close]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 212/241 (87.97%), Postives = 222/241 (92.12%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAF FYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVGLD 150
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SP VTS+S D  YDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 151 TELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEM 210
           TELDFLRNKLSEVRKENIVLNQELQALERQ A+SNSQ+N FNEALQLYE+NSVNEMFQEM
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 211 MGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKT 270
           MGTASELRAKIGKLKKRRMEESKLTKVEK+HTNGD SH+H G SNAKLDDI+ FL DLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

[Close]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.8e-109
Identity = 213/242 (88.02%), Postives = 222/242 (91.74%), Query Frame = 0


Query: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAF FYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHD--YDVGLD 150
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SP VTS+S D  YDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 151 TELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQ-E 210
           TELDFLR  LSEVRKENIVLNQELQALERQTA+SNSQ+N FNEALQLYEQNSVNEMFQ E
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQAE 180

Query: 211 MMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLK 270
           MMGTASELRAKIGKLKKRRMEESKLTKVEK+HTN DISH+H G SNAKLDDI+ FL DLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLK 240

The following BLAST results are available for this feature:

BLAST of Bhi02G000026 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	1.6e-53	47.74	minichromosome instability 12 (mis12)-like	[more]

BLAST of Bhi02G000026 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	2.3e-52	47.74	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Bhi02G000026 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879945.1	2.9e-145	100.00	protein MIS12 homolog [Benincasa hispida]	[more]
XP_023515499.1	1.1e-112	89.21	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAA0058032.1	5.5e-112	88.28	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
KAG6589575.1	7.2e-112	88.80	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0058004.1	9.4e-112	88.28	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]

BLAST of Bhi02G000026 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UQ95	2.7e-112	88.28	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A5A7US57	4.5e-112	88.28	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1JME3	1.1e-110	88.38	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1E0A1	1.1e-110	87.97	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JD43	2.8e-109	88.02	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 151..178
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 41..169 e-value: 1.9E-18 score: 66.9
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 31..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000026	Bhi02M000026	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus