Bhi02G000019 (gene) Wax gourd (B227) v1

Overview


Name	Bhi02G000019
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	AMP-binding domain-containing protein
Location	chr2: 606695 .. 607359 (+)
RNA-Seq Expression	Bhi02G000019
Synteny	Bhi02G000019

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGTAATCAAAGATTGACTTGCTTTTCTTAATTATTTGAACTAGCCGCTAGCACTACTGAAAAACGTTATAATCATAATAAATATCACGTGGGGTGTTAAGATTCCTGCAATTTTTTTTTCTATTTAGATTGTTAGCAAAAGATACAGTGAAATCTCATTTGATTCACATTCGTGATTAGGGAGAATGCGGCGTTCACCAATGTTGGCCAGCTATTAGAGAGGCGAGGAAAGGAGTTTTTGGGGTCAAATTATAGAGACCCGTTATCAAGTTTCTCTTCTTTTCAGGAGTTTCGGTCTCAAACCCCGAGGTACTGTGAGTTTTAAATTAAAGTTTGTAATCCAACTTTGTTTCTATTAATGTTTGGATTAAACTTGTCTTCTGTTTGTCAGGTTTATTGGAGAACTGTGCTAGATGAGATGCACATAACAGTTTCTAGACCTCCAAAATGTATCTTGCAAATGAATGGATCTCCAGAAAGCCAGTCTCCAAGTCCTGGTTGTCAATGGCTTCCTGGAGCAGTATTTAATCCAGCTAAAAATTGCTTGTCTTTAAATAAAAACAGGAGTTTAGATGATGTAGCAATCATTTGGCGTGATGAAGGAAGTGATAACCGGCCTGTGAAGAGATTGACTGTTGGAGAATTGCGTACAGAGGTTTG

mRNA sequence

ATGGCGGGAGTTTCGGTCTCAAACCCCGAGGTTTATTGGAGAACTGTGCTAGATGAGATGCACATAACAGTTTCTAGACCTCCAAAATGTATCTTGCAAATGAATGGATCTCCAGAAAGCCAGTCTCCAAGTCCTGGTTGTCAATGGCTTCCTGGAGCAGTATTTAATCCAGCTAAAAATTGCTTGTCTTTAAATAAAAACAGGAGTTTAGATGATGTAGCAATCATTTGGCGTGATGAAGGAAGTGATAACCGGCCTGTGAAGAGATTGACTGTTGGAGAATTGCGTACAGAGGTTTG

Coding sequence (CDS)

ATGGCGGGAGTTTCGGTCTCAAACCCCGAGGTTTATTGGAGAACTGTGCTAGATGAGATGCACATAACAGTTTCTAGACCTCCAAAATGTATCTTGCAAATGAATGGATCTCCAGAAAGCCAGTCTCCAAGTCCTGGTTGTCAATGGCTTCCTGGAGCAGTATTTAATCCAGCTAAAAATTGCTTGTCTTTAAATAAAAACAGGAGTTTAGATGATGTAGCAATCATTTGGCGTGATGAAGGAAGTGATAACCGGCCTGTGAAGAGATTGACTGTTGGAGAATTGCGTACAGAGGTTTG

Protein sequence

MAGVSVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSLNKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV

Homology

[Close]

BLAST of Bhi02G000019 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 55/95 (57.89%), Postives = 71/95 (74.74%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYW+TVLDE++I  S PPKCIL+ + S +    +PG +WLPGA  NPA+NCL+ 
Sbjct: 122 SVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNCLTN 181

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
              R LDD+ I WRDEGSD+ PV  +T+ ELR++V
Sbjct: 182 GFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQV 212

[Close]

BLAST of Bhi02G000019 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 84.3 bits (207), Expect = 5.8e-17
Identity = 42/97 (43.30%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCL-- 64
           SV +PEVYW  V+DE+ +    PP+CIL  +         P   WLP AV N A+ CL  
Sbjct: 128 SVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNIAECCLMP 187

Query: 65  SLNKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           S +  +  D VA++WR+EG D+ PV R+T+ ELR +V
Sbjct: 188 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQV 216

[Close]

BLAST of Bhi02G000019 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.5e-25
Identity = 55/95 (57.89%), Postives = 71/95 (74.74%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYW+TVLDE++I  S PPKCIL+ + S +    +PG +WLPGA  NPA+NCL+ 
Sbjct: 122 SVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGD----NPGGKWLPGAYLNPARNCLTN 181

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
              R LDD+ I WRDEGSD+ PV  +T+ ELR++V
Sbjct: 182 GFKRRLDDIVIRWRDEGSDDLPVNTMTLLELRSQV 212

[Close]

BLAST of Bhi02G000019 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.2e-16
Identity = 42/97 (43.30%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCL-- 64
           SV +PEVYW  V+DE+ +    PP+CIL  +         P   WLP AV N A+ CL  
Sbjct: 128 SVEHPEVYWSFVIDELSLVFHTPPRCILNKS--------KPEGTWLPDAVLNIAECCLMP 187

Query: 65  SLNKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           S +  +  D VA++WR+EG D+ PV R+T+ ELR +V
Sbjct: 188 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQV 216

[Close]

BLAST of Bhi02G000019 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.3e-10
Identity = 42/98 (42.86%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCL-- 64
           SV  PE YW  VL E+ ++    PKCIL  +     +S S     LPG+V N A+ CL  
Sbjct: 124 SVQQPEAYWSIVLKELSVSFQEAPKCILD-SADKSKRSGS----LLPGSVLNIAECCLLP 183

Query: 65  -SLNKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
            S    R  D  AI+WRDEG D+  V  LT+ ELR +V
Sbjct: 184 TSYPMKRD-DSPAIVWRDEGYDDSEVNVLTLKELREKV 215

[Close]

BLAST of Bhi02G000019 vs. NCBI nr
Match: XP_038880762.1 (probable acyl-activating enzyme 17, peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 185.3 bits (469), Expect = 2.6e-43
Identity = 87/95 (91.58%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRTVLDEMHIT SRPPKCILQ NGSPESQSPSPG +WLPGAVFNPAK+CLSL
Sbjct: 156 SVSNPEVYWRTVLDEMHITFSRPPKCILQRNGSPESQSPSPGGRWLPGAVFNPAKDCLSL 215

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           NKNRSLDDVAIIWRDEG DN PVKRLTVGELRT+V
Sbjct: 216 NKNRSLDDVAIIWRDEGCDNLPVKRLTVGELRTDV 250

[Close]

BLAST of Bhi02G000019 vs. NCBI nr
Match: XP_038880763.1 (probable acyl-activating enzyme 17, peroxisomal isoform X2 [Benincasa hispida])

HSP 1 Score: 185.3 bits (469), Expect = 2.6e-43
Identity = 87/95 (91.58%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRTVLDEMHIT SRPPKCILQ NGSPESQSPSPG +WLPGAVFNPAK+CLSL
Sbjct: 156 SVSNPEVYWRTVLDEMHITFSRPPKCILQRNGSPESQSPSPGGRWLPGAVFNPAKDCLSL 215

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           NKNRSLDDVAIIWRDEG DN PVKRLTVGELRT+V
Sbjct: 216 NKNRSLDDVAIIWRDEGCDNLPVKRLTVGELRTDV 250

[Close]

BLAST of Bhi02G000019 vs. NCBI nr
Match: XP_022135246.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 170.6 bits (431), Expect = 6.6e-39
Identity = 80/95 (84.21%), Postives = 86/95 (90.53%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRT+LDEMHIT S+PP CILQMN S ESQ  SPG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           N+NRSLDDVAIIWRDEG DN PVKRLT+GELRT+V
Sbjct: 183 NENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDV 217

[Close]

BLAST of Bhi02G000019 vs. NCBI nr
Match: XP_022135245.1 (probable acyl-activating enzyme 17, peroxisomal [Momordica charantia])

HSP 1 Score: 170.2 bits (430), Expect = 8.7e-39
Identity = 80/95 (84.21%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRTVLDEMHIT S+PP CILQMN SPESQ  SPG QWLPGAVFNPAK+CLS+
Sbjct: 123 SVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCLSI 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           N+NRSLDDVAIIWRDEG DN PVKRLT+ ELR EV
Sbjct: 183 NENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEV 217

[Close]

BLAST of Bhi02G000019 vs. NCBI nr
Match: KAG6587638.1 (putative acyl-activating enzyme 17, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 168.3 bits (425), Expect = 3.3e-38
Identity = 76/94 (80.85%), Postives = 84/94 (89.36%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYW+ VLD+MHIT S+PPKCILQMN SP+ QSP PG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTE 99
           N+NRSLDD A+IWRDEG DN PVKRLT+ ELRTE
Sbjct: 183 NENRSLDDAAVIWRDEGCDNMPVKRLTLRELRTE 216

[Close]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL
Match: A0A6J1C048 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007256 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.2e-39
Identity = 80/95 (84.21%), Postives = 86/95 (90.53%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRT+LDEMHIT S+PP CILQMN S ESQ  SPG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWRTMLDEMHITFSKPPHCILQMNDSTESQFSSPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           N+NRSLDDVAIIWRDEG DN PVKRLT+GELRT+V
Sbjct: 183 NENRSLDDVAIIWRDEGCDNLPVKRLTLGELRTDV 217

[Close]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL
Match: A0A6J1C0M0 (probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111007255 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 4.2e-39
Identity = 80/95 (84.21%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRTVLDEMHIT S+PP CILQMN SPESQ  SPG QWLPGAVFNPAK+CLS+
Sbjct: 123 SVSNPEVYWRTVLDEMHITFSKPPNCILQMNDSPESQFRSPGGQWLPGAVFNPAKDCLSI 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           N+NRSLDDVAIIWRDEG DN PVKRLT+ ELR EV
Sbjct: 183 NENRSLDDVAIIWRDEGCDNLPVKRLTLRELRAEV 217

[Close]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL
Match: A0A6J1EU22 (probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111437755 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.6e-38
Identity = 76/94 (80.85%), Postives = 84/94 (89.36%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYW+ VLD+MHIT S+PPKCILQMN SP+ QSP PG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWKAVLDDMHITFSKPPKCILQMNDSPDCQSPCPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTE 99
           N+NRSLDD A+IWRDEG DN PVKRLT+ ELRTE
Sbjct: 183 NENRSLDDAAVIWRDEGCDNMPVKRLTLRELRTE 216

[Close]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL
Match: A0A6J1I6W7 (probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111471706 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.9e-37
Identity = 75/94 (79.79%), Postives = 83/94 (88.30%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYW+ VLD+M IT S+PPKCILQMN SP+ QSP PG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWKAVLDDMQITFSKPPKCILQMNDSPDYQSPWPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTE 99
           N+NRSLDD A+IWRDEG DN PVKRLT+ ELRTE
Sbjct: 183 NENRSLDDAALIWRDEGCDNMPVKRLTLRELRTE 216

[Close]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL
Match: A0A0A0LUS2 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G015740 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-36
Identity = 76/95 (80.00%), Postives = 85/95 (89.47%), Query Frame = 0


Query: 5   SVSNPEVYWRTVLDEMHITVSRPPKCILQMNGSPESQSPSPGCQWLPGAVFNPAKNCLSL 64
           SVSNPEVYWRTVLDEM+IT S+PP CILQ+N SPES+  SPG QWLPGAVFNPAK+CLSL
Sbjct: 123 SVSNPEVYWRTVLDEMNITFSKPPNCILQINDSPESKFSSPGGQWLPGAVFNPAKDCLSL 182

Query: 65  NKNRSLDDVAIIWRDEGSDNRPVKRLTVGELRTEV 100
           N+NRSLDDVAIIWRDEG D+ P+KRLT+ ELR EV
Sbjct: 183 NENRSLDDVAIIWRDEGYDDLPLKRLTLRELRAEV 217

The following BLAST results are available for this feature:

BLAST of Bhi02G000019 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23050.1	1.1e-26	57.89	acyl-activating enzyme 17	[more]
AT1G55320.1	5.8e-17	43.30	acyl-activating enzyme 18	[more]

BLAST of Bhi02G000019 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBF3	1.5e-25	57.89	Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 ...	[more]
Q84P17	8.2e-16	43.30	Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 ...	[more]
M4IRL9	2.3e-10	42.86	Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1	[more]

BLAST of Bhi02G000019 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880762.1	2.6e-43	91.58	probable acyl-activating enzyme 17, peroxisomal isoform X1 [Benincasa hispida]	[more]
XP_038880763.1	2.6e-43	91.58	probable acyl-activating enzyme 17, peroxisomal isoform X2 [Benincasa hispida]	[more]
XP_022135246.1	6.6e-39	84.21	probable acyl-activating enzyme 17, peroxisomal [Momordica charantia]	[more]
XP_022135245.1	8.7e-39	84.21	probable acyl-activating enzyme 17, peroxisomal [Momordica charantia]	[more]
KAG6587638.1	3.3e-38	80.85	putative acyl-activating enzyme 17, peroxisomal, partial [Cucurbita argyrosperma...	[more]

BLAST of Bhi02G000019 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C048	3.2e-39	84.21	probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C0M0	4.2e-39	84.21	probable acyl-activating enzyme 17, peroxisomal OS=Momordica charantia OX=3673 G...	[more]
A0A6J1EU22	1.6e-38	80.85	probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1I6W7	3.9e-37	79.79	probable acyl-activating enzyme 17, peroxisomal OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0LUS2	1.1e-36	80.00	AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G015740...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 1..99 e-value: 1.2E-10 score: 42.7
None	No IPR available	PANTHER	PTHR44378	ACYL-ACTIVATING ENZYME 17, PEROXISOMAL-RELATED	coord: 4..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi02M000019	Bhi02M000019	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane