Bhi01G002663 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G002663
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr1: 86814510 .. 86817046 (-)
RNA-Seq Expression	Bhi01G002663
Synteny	Bhi01G002663

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAATAATTCAATTGACATCAAGAACAGAACTATTATTGCTAAAGCCAAAGGCATGGGAATTCAATCTTCAGCAAAGTCAGAAGTTCCTGTTATCAAATTCTCCATTGAAAATGATGAAGATCTTCATCCTCAAAGCACCAAATGGTTAGAAACATGTAGCCAAGTGAGAGCTGCTCTTGAAGAATATGGCTGTTTTTTAGCTGAGTATGAGAGTGCCTCATCAAAGCTTTATGATGAAATTTTCAAAGGCTTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATGAAAAATGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATACAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCAACATCTTTTCAAGCTGTTGAGTCTTTTCTTAAGCTCTTGTGGCCTTCTGGAAATCATCATTTCTGGTAAATTACTATAACTGGATTTATATTATGGTTGATAGTAATATGCGCATTTTTTTTTCTTTTGTAATCTGAATCTTTTGTTTTTTTAATGTTCTTCATCTCATTCATTCATTTCATCATATATATATATCTAAGTTAATTGTAATTGATAACAACTATTGTGTTTAATCTTTTTGAGTATGTTTGCATCGCAGGTAGTTTTTATAAAAAAATGAATCAAAATATTTACAAATATAGCAAAATTTTACTGTGGTAAGTCACGATAAACTACTATTTATATCTTTCTGTGTCATGATAAACACAAAGTAGTCTTGTGATATATTTATATTATTTATAAATATTTTTAGTAGTTTTGTCATTTAAAATAATTTTTAAAAAAATCATAAATTATTTTTTCTAATTTTCAAAACCTGATTTGGTTTGTGAAACTATAAGTAGACTATACAACAAAAGAAAATAGGGTAAAAGTAGAGTTTAGAATCTTAATATTTAAAAAATTAAATAAATTGGTTAGCAAAGGTGCCTAAAATTTTCAAAAATAATTTTTCCCCCCAAAAAGAATAGAAAACGAAAGAATGACTCTTAATTTGTCATGGTAATTAGTTTTGAATGGAAAAGAGAAAAATAGGAAAAATAGGAGTATTTCTCATTTATTCCTTTGTGGGTTTATTCATTTTGAAAAAAATAAAATTATTGTTGGACAGTGAAAATCTATTTTCATACTCAGAGATGATATCAGAGATGGAGCAATTAGTAAAAAAGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTACCTTCTAAGAACAATGAAATATAGAATTCCTCAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCATACAGACAAGAGTTTCTTCACTATTCTTCATCAAATTAATGGAGTCAATGGCTTGGAAATCAAACCAAAAAATGGTGATCATTGGCTTCGACTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTATGCCCTCTCATTTCTTCTCTTTTTACGTCTCGGATAATTATTAGTAGTATTTTTAAGAATAATAACTAAATGTATAACATTATTTAAAAAAAATTACAAATATAGACAAATCTATCGACGATTGGCCATATTTGCATTTTTTATATATGCTAATACTTTGGGTCTAATGTACAAATATAGACATTAAGTTTGGCCATATTGAATTTTTTTTAAATTGATGTTATACATTTAGTTATTATTTTTAAAAATGCTACCAATTACAATTACCTAATTAATACTTTTTTTCCTTTAATGTTCTTGAAATTTAGTATTGTATTTTGTAGAAATAGGAATAGTTACCCAAACAAATGTATAGTTTTTTTTTTTTAAACATTCTTCTAACTTGTCTGGAGTGAAGTTGACTATTATAATCCAATAAATATTTGGACCTCCCATTATAATATTTGAGATTTCTAATTATTATAACCCACACCTAGCTACACTAAATCTATTTGAAAATCCTCATTGTTTATAATGTAACTATTTCGTATAACCGTCGTCTTCCTCATTTGTAATTTAAACTAAAATAGTCTACATCTCAATCTAATCGACCCTCTAAACCTCTCTAAATGCTTATTTTTTTGGATAGGCATGGAGCAATGGGAGAATCCACTCAGCCACCCATAGAGTTATCATTGAGGGAAGCAAGGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGCAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTACTTTTCCACAGATGAGTGTGAGAAAATGCCTTCCACTGCCAATGCTTATTGTGGAGTTTGAGAGACTTTATATGCCAAATATTATATGTATGGTTGTTGGTGGAATAATCAATTTTCATTATAAACAAACTTATATCACAATATATATATAAAGAAAGCCACGTTACGTATGTCTATATATTGCCAAACTTTTATTTAATTTTCAAGTTGAATTATAAAGCTTATTAATTTTATGTATTTGGG

mRNA sequence

GCAAATAATTCAATTGACATCAAGAACAGAACTATTATTGCTAAAGCCAAAGGCATGGGAATTCAATCTTCAGCAAAGTCAGAAGTTCCTGTTATCAAATTCTCCATTGAAAATGATGAAGATCTTCATCCTCAAAGCACCAAATGGTTAGAAACATGTAGCCAAGTGAGAGCTGCTCTTGAAGAATATGGCTGTTTTTTAGCTGAGTATGAGAGTGCCTCATCAAAGCTTTATGATGAAATTTTCAAAGGCTTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATGAAAAATGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATACAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCAACATCTTTTCAAGCTGTTGAGTCTTTTCTTAAGCTCTTGTGGCCTTCTGGAAATCATCATTTCTGTGAAAATCTATTTTCATACTCAGAGATGATATCAGAGATGGAGCAATTAGTAAAAAAGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTACCTTCTAAGAACAATGAAATATAGAATTCCTCAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCATACAGACAAGAGTTTCTTCACTATTCTTCATCAAATTAATGGAGTCAATGGCTTGGAAATCAAACCAAAAAATGGTGATCATTGGCTTCGACTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGCATGGAGCAATGGGAGAATCCACTCAGCCACCCATAGAGTTATCATTGAGGGAAGCAAGGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGCAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTACTTTTCCACAGATGAGTGTGAGAAAATGCCTTCCACTGCCAATGCTTATTGTGGAGTTTGAGAGACTTTATATGCCAAATATTATATGTATGGTTGTTGGTGGAATAATCAATTTTCATTATAAACAAACTTATATCACAATATATATATAAAGAAAGCCACGTTACGTATGTCTATATATTGCCAAACTTTTATTTAATTTTCAAGTTGAATTATAAAGCTTATTAATTTTATGTATTTGGG

Coding sequence (CDS)

GCAAATAATTCAATTGACATCAAGAACAGAACTATTATTGCTAAAGCCAAAGGCATGGGAATTCAATCTTCAGCAAAGTCAGAAGTTCCTGTTATCAAATTCTCCATTGAAAATGATGAAGATCTTCATCCTCAAAGCACCAAATGGTTAGAAACATGTAGCCAAGTGAGAGCTGCTCTTGAAGAATATGGCTGTTTTTTAGCTGAGTATGAGAGTGCCTCATCAAAGCTTTATGATGAAATTTTCAAAGGCTTGAAAGAGCTTTTTGATTTGCCATTAGAAACTAAAATGAAAAATGTTTCTGATAAGCCTTACCATGGATTTTTGGACCATACAGCTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGTATTGAAAATGCAACATCTTTTCAAGCTGTTGAGTCTTTTCTTAAGCTCTTGTGGCCTTCTGGAAATCATCATTTCTGTGAAAATCTATTTTCATACTCAGAGATGATATCAGAGATGGAGCAATTAGTAAAAAAGATGGTATTTGAAAGCTATGGAGTGGGAAAATACTATGATTCTCATATTGCTTCAACCACTTACCTTCTAAGAACAATGAAATATAGAATTCCTCAAATGGGTGAAGAGAATATTGGTGCAGAAGCTCATACAGACAAGAGTTTCTTCACTATTCTTCATCAAATTAATGGAGTCAATGGCTTGGAAATCAAACCAAAAAATGGTGATCATTGGCTTCGACTTCAATTCTCTCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGCATGGAGCAATGGGAGAATCCACTCAGCCACCCATAGAGTTATCATTGAGGGAAGCAAGGAAAGATATTCAACTGCCCTATTTTCATACCACAAAGGAATTATTGAAATTCCACAAGAATTGGTGGATGAGCAACATCCCTTGAGATTTAACCCTTTTAACCATTATGGATTACTTGGTTACTTTTCCACAGATGAGTGTGAGAAAATGCCTTCCACTGCCAATGCTTATTGTGGAGTTTGA

Protein sequence

ANNSIDIKNRTIIAKAKGMGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDECEKMPSTANAYCGV

Homology

[Close]

BLAST of Bhi01G002663 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 310.5 bits (794), Expect = 1.7e-84
Identity = 139/313 (44.41%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 27  SEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFKGLK 86
           ++VP++   + + ++L P ++ W     +   ALEEYGCFLA Y+  + +L D IF   +
Sbjct: 8   TKVPIL--DLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 67

Query: 87  ELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGN 146
           ELFDLP ETK KNV++KPYHG++      V+PLH+ LG++  T+ +  + F  L+WP GN
Sbjct: 68  ELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGN 127

Query: 147 HHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEEN 206
             FC  + ++S  ++E+++LV +M+FE+YGV K+Y+SH+ S TYLL+ +KY  P      
Sbjct: 128 DRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISM 187

Query: 207 IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRI 266
                HTDK+F +ILHQ N VNGLE+K K+G+ W+ LQ  P S++VMAGD  + WSN RI
Sbjct: 188 PAFPQHTDKTFLSILHQ-NDVNGLEVKSKDGE-WISLQLPPKSYVVMAGDISMGWSNDRI 247

Query: 267 HSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDEC 326
            S  HRV +EG K RY+  LFS+   ++ IP+ELVD++HPL + PF++  L+ +++T E 
Sbjct: 248 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 307

Query: 327 EKMPSTANAYCGV 340
            +  ST  AYCG+
Sbjct: 308 REANSTLKAYCGI 314

[Close]

BLAST of Bhi01G002663 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 308.5 bits (789), Expect = 6.5e-84
Identity = 147/312 (47.12%), Postives = 204/312 (65.38%), Query Frame = 0


Query: 28  EVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASS-KLYDEIFKGLK 87
           ++P + FS    EDL P +  W  T   +R ALEEYGCF+ + +  +   L D +F  L 
Sbjct: 4   KIPTLDFS---REDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLV 63

Query: 88  ELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGN 147
           +LFDLP +TKMKN  DKP +G++       LPLH+SLGI+NATS +A  SF  L+WP GN
Sbjct: 64  DLFDLPTQTKMKNKYDKPLNGYVGQIP--ALPLHESLGIDNATSLEATRSFTGLMWPQGN 123

Query: 148 HHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEEN 207
            HF E L+ Y+E  +E++Q+V +MVF+SY V KYYD +I STTYLLR +K R P      
Sbjct: 124 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 183

Query: 208 IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRI 267
           +G   HTDKSF TILHQ + VNGLE++ + G+       SP+ F+V+AGDA +AWSN R+
Sbjct: 184 LGFVTHTDKSFTTILHQ-DQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRV 243

Query: 268 HSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDEC 327
            S  H+V++ G  +RYS  +F+++ G +++P+EL+D QHPL + PF+H GLL ++ TD  
Sbjct: 244 WSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTG 303

Query: 328 EKMPSTANAYCG 339
            K      AYCG
Sbjct: 304 YKSECPIKAYCG 309

[Close]

BLAST of Bhi01G002663 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 5.7e-72
Identity = 131/313 (41.85%), Postives = 203/313 (64.86%), Query Frame = 0


Query: 29  VPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFKGLKEL 88
           +PVI FS +N   L P   +W  T + V+ AL++YG F A ++    +L   +F  L+EL
Sbjct: 12  LPVIDFSNKN---LKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEEL 71

Query: 89  FDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGNHH 148
           FDLPL+TK++NVS KP+HG++      ++PL++S+GI+++   + V++F + LWP GN  
Sbjct: 72  FDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAFTEKLWPQGNIS 131

Query: 149 FCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEENIG 208
           F   + S+S+ +SE++  +++M+ ES+G+ KY D H+ ST YLLR MKY+ P   E  +G
Sbjct: 132 FSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVG 191

Query: 209 AEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRIHS 268
             AHTDK+  TIL+Q N V GLE++ K+  +W++++ + +SF VM GD+  A  NGR+HS
Sbjct: 192 LNAHTDKNIVTILYQ-NHVEGLEVQTKD-KNWIKVKPTQDSFTVMIGDSLYALLNGRLHS 251

Query: 269 ATHRVIIEGSKERYSTALFSYHKG--IIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDEC 328
             HRV++ G++ RYS  LFS  K   I+  P ELVDE+HP  F PF+H   L ++ T+  
Sbjct: 252 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 311

Query: 329 EKMPSTANAYCGV 340
           ++  S    YCG+
Sbjct: 312 QRSQSALKTYCGI 317

[Close]

BLAST of Bhi01G002663 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 263.1 bits (671), Expect = 3.1e-70
Identity = 134/317 (42.27%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 28  EVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFKGLKE 87
           E+P+I  S   D++L   +  W  T   VR A+E +G F+AEY +  + L+  I +  KE
Sbjct: 8   ELPIINLS---DKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKE 67

Query: 88  LFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWP---S 147
           L DLP+E K+KN + K  HG++   +    P+H+ LGI+     Q    F +L+WP    
Sbjct: 68  LLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHD 127

Query: 148 GNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGE 207
            N  FCE + +Y++M +E+EQLV +M+FESY V KY + +I  T YLLR +KYR    GE
Sbjct: 128 DNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGE 187

Query: 208 ENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNG 267
            N    +HTDKSF +ILHQ N + GL +K +  D W     SP  F+V+AGDA +AWSN 
Sbjct: 188 PNRKFISHTDKSFISILHQ-NHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSND 247

Query: 268 RIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTD 327
           RI +  H+V +E  + RYS   FS+ +G+I  P+E+VD+ HPL +NPF+H GLL Y+ T 
Sbjct: 248 RIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETL 307

Query: 328 E--CEKMPSTANAYCGV 340
           E   +   +   AYCG+
Sbjct: 308 EVHLKAHRTMTKAYCGI 319

[Close]

BLAST of Bhi01G002663 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 7.0e-70
Identity = 136/325 (41.85%), Postives = 200/325 (61.54%), Query Frame = 0


Query: 21  IQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDE 80
           +  S   ++PVI FS   D++L P S+KW E  + V  ALE+YGCF A ++  S +L   
Sbjct: 6   VPPSVSFQLPVIDFS---DQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRS 65

Query: 81  IFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKL 140
           +F+ +++LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F + 
Sbjct: 66  VFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQ 125

Query: 141 LWPS-GNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRI 200
           LWP  GN    E +  +SE + E++ +V++M+ ES+G+  Y D H+ ST YL R MKY  
Sbjct: 126 LWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTS 185

Query: 201 PQMGEEN------IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVM 260
           P   +++      +G  +HTDK+  TILHQ   V+GLE+K K+ D W++++ S +S LVM
Sbjct: 186 PPDDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSVLVM 245

Query: 261 AGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPQELVDEQHPLRFNP 320
            GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD++HP  F P
Sbjct: 246 VGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKP 305

Query: 321 FNHYGLLGYFSTDECEKMPSTANAY 337
           F +   L +F T+      S  +A+
Sbjct: 306 FEYTDFLHFFQTEAGRIAQSALHAF 319

[Close]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 9.8e-69
Identity = 136/325 (41.85%), Postives = 200/325 (61.54%), Query Frame = 0


Query: 21  IQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDE 80
           +  S   ++PVI FS   D++L P S+KW E  + V  ALE+YGCF A ++  S +L   
Sbjct: 6   VPPSVSFQLPVIDFS---DQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRS 65

Query: 81  IFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKL 140
           +F+ +++LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F + 
Sbjct: 66  VFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQ 125

Query: 141 LWPS-GNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRI 200
           LWP  GN    E +  +SE + E++ +V++M+ ES+G+  Y D H+ ST YL R MKY  
Sbjct: 126 LWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTS 185

Query: 201 PQMGEEN------IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVM 260
           P   +++      +G  +HTDK+  TILHQ   V+GLE+K K+ D W++++ S +S LVM
Sbjct: 186 PPDDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSVLVM 245

Query: 261 AGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPQELVDEQHPLRFNP 320
            GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD++HP  F P
Sbjct: 246 VGDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKP 305

Query: 321 FNHYGLLGYFSTDECEKMPSTANAY 337
           F +   L +F T+      S  +A+
Sbjct: 306 FEYTDFLHFFQTEAGRIAQSALHAF 319

[Close]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-68
Identity = 136/324 (41.98%), Postives = 201/324 (62.04%), Query Frame = 0


Query: 21  IQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDE 80
           +  S   ++PVI FS   D++L P S+KW E  + V  ALE+YGCF A ++  S +L   
Sbjct: 6   VPPSVSFQLPVIDFS---DQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRS 65

Query: 81  IFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKL 140
           +F+ +++LF+LP+ TK +NVS KP+HG+L H       L++SLGI++A   + V  F + 
Sbjct: 66  VFEAMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQ 125

Query: 141 LWPS-GNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRI 200
           LWP  GN    E +  +SE + E++ +V++M+ ES+G+  Y D H+ ST YL R MKY  
Sbjct: 126 LWPDHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTS 185

Query: 201 PQMGEEN-----IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMA 260
           P   +++     +G  +HTDK+  TILHQ   V+GLE+K K+ D W++++ S +S LVM 
Sbjct: 186 PPDDDDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSVLVMV 245

Query: 261 GDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPQELVDEQHPLRFNPF 320
           GD+  A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD++HP  F PF
Sbjct: 246 GDSLCALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPF 305

Query: 321 NHYGLLGYFSTDECEKMPSTANAY 337
            +   L +F T+      S  +A+
Sbjct: 306 EYTDFLHFFQTEAGRIAQSALHAF 318

[Close]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 134/308 (43.51%), Postives = 195/308 (63.31%), Query Frame = 0


Query: 24  SAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFK 83
           S   ++PVI FS   D++L P S+KW E  + V  ALE+YGCF A ++  S +L   +F+
Sbjct: 2   SVSFQLPVIDFS---DQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFE 61

Query: 84  GLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWP 143
            +++LF+LP+ TK +NVS K +HG+L H       L++SLGI +A   + V  F + LWP
Sbjct: 62  AMEDLFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWP 121

Query: 144 S-GNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQM 203
             GN    E +  +SE + E++ +V++M+ ES+G+ KY D H+ ST YL R MKY  P  
Sbjct: 122 DHGNKSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPD 181

Query: 204 GEEN----IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC 263
            +++    +G  +HTDK+  TILHQ   V+GLE+K K+ D W++++ S +S LVM GD+ 
Sbjct: 182 DDDDEETKLGLRSHTDKNIITILHQYQ-VDGLEVKTKD-DKWIKVKPSQDSVLVMVGDSL 241

Query: 264 LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--IIEIPQELVDEQHPLRFNPFNHYG 323
            A  NGR+HS  HRVI+ G K RYST LFS  K   II+ P+ELVD++HP  F PF +  
Sbjct: 242 CALLNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTD 298

Query: 324 LLGYFSTD 325
            L +F T+
Sbjct: 302 FLHFFQTE 298

[Close]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-63
Identity = 130/306 (42.48%), Postives = 184/306 (60.13%), Query Frame = 0


Query: 23  SSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIF 82
           S+A   +PVI  S++N   L P +T W    +QVR ALEEYGCF A  ++ S +L   + 
Sbjct: 3   STAPLRLPVIDLSMKN---LKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVC 62

Query: 83  KGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLW 142
               EL +LPLETKM N +   Y GF       +  L++ +G+   T  + VE F  L+W
Sbjct: 63  NKGHELLNLPLETKMLNGNKPEYDGFTS-----IPNLNEGMGVGRITDLEKVERFTNLMW 122

Query: 143 PSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQM 202
           P GN  FCE ++SY + ++E++ ++K MVFES+G+ K++DS   ST YLL  M+Y+ P  
Sbjct: 123 PEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGK 182

Query: 203 GEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWS 262
              +     H DKS  TI++Q N V GLE + K+G+ W+    + ++ +V+ GD  +AWS
Sbjct: 183 DGRSPALSLHKDKSILTIVNQ-NDVKGLEFETKDGE-WILP--TADNHIVLLGDCFMAWS 242

Query: 263 NGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFS 322
           NGR+HS  HRV +  ++ R ST+ FS+ K IIE P ELVDE+HPL FNPF    LL Y  
Sbjct: 243 NGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCF 296

Query: 323 TDECEK 329
           T E  K
Sbjct: 303 TKEGAK 296

[Close]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.7e-55
Identity = 135/437 (30.89%), Postives = 202/437 (46.22%), Query Frame = 0


Query: 24  SAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIFK 83
           S   ++P+I  +   D+ L P S+KW E  S VR ALE++GCF A Y+  S +L + I K
Sbjct: 3   SCSLQLPLINLA---DKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMK 62

Query: 84  GLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWP 143
            ++ELF LP+ETK +NV  KPY G+L+H       L +SLGI NA   + V  F + LWP
Sbjct: 63  TMEELFALPVETKQRNVCPKPYVGYLNHN-----NLSESLGISNANILENVNEFTQQLWP 122

Query: 144 --SGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQ 203
              GN +  + +  ++E + E++ +V++MV ES+G+ KY D H+ ST Y +R MKY  P 
Sbjct: 123 DGDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKYIAPP 182

Query: 204 MGEEN------------------------------------------------------- 263
            G+ N                                                       
Sbjct: 183 EGDANTTVDDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVN 242

Query: 264 ------------------------------------------------------------ 323
                                                                       
Sbjct: 243 DNLNVDAEANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEK 302

Query: 324 -IGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGR 340
            +G   HTDK+ FT+L Q + + GLE+K K+ + W+R++ SPN+F+V+AGD+  A  NGR
Sbjct: 303 KLGLPCHTDKNLFTVLFQ-HEIEGLEVKTKD-EKWIRVKPSPNTFIVIAGDSLCALMNGR 362

[Close]

BLAST of Bhi01G002663 vs. NCBI nr
Match: XP_038878200.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida])

HSP 1 Score: 673.7 bits (1737), Expect = 8.3e-190
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 78
           MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY
Sbjct: 1   MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 60

Query: 79  DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL 138
           DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL
Sbjct: 61  DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL 120

Query: 139 KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR 198
           KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR
Sbjct: 121 KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR 180

Query: 199 IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC 258
           IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC
Sbjct: 181 IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC 240

Query: 259 LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLL 318
           LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLL
Sbjct: 241 LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLL 300

Query: 319 GYFSTDECEKMPSTANAYCGV 340
           GYFSTDECEKMPSTANAYCGV
Sbjct: 301 GYFSTDECEKMPSTANAYCGV 321

[Close]

BLAST of Bhi01G002663 vs. NCBI nr
Match: KAG6606775.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.4 bits (1435), Expect = 8.6e-155
Identity = 263/324 (81.17%), Postives = 291/324 (89.81%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYES---ASS 78
           MG +SS K EVPVI FSIEN   L P+STKW+ETC+ VRAALEEYGCFLAEYE+      
Sbjct: 1   MGFRSSTKPEVPVINFSIEN---LRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEV 60

Query: 79  KLYDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVE 138
           +L D IF GL++LFDLPL+TKMKNVSDKPYHGFLDH APFVLPLH+S GIENA SF+ VE
Sbjct: 61  ELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVE 120

Query: 139 SFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTM 198
           SF+KL+WPSGNHHFC++LF+Y++M+SEME+LVK+MVFESYGVGKYYDSHIASTTYLLR M
Sbjct: 121 SFVKLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGM 180

Query: 199 KYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAG 258
           KYRIP+MGE+NIGAEAHTDKSFFTILHQ NGVNGLEIK K  D W+RLQF+PNSFLVMAG
Sbjct: 181 KYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDRWIRLQFNPNSFLVMAG 240

Query: 259 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHY 318
           DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHY
Sbjct: 241 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHY 300

Query: 319 GLLGYFSTDECEKMPSTANAYCGV 340
           GLLGYFS +ECEK PSTA AYCGV
Sbjct: 301 GLLGYFSKEECEKTPSTAGAYCGV 320

[Close]

BLAST of Bhi01G002663 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.1 bits (1429), Expect = 4.3e-154
Identity = 263/324 (81.17%), Postives = 289/324 (89.20%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYES---ASS 78
           MG +SS   EVPVI FSIEN   L P+STKW+ETCS VRAALEEYGCFLAEYE+      
Sbjct: 1   MGFRSSTNPEVPVISFSIEN---LRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEV 60

Query: 79  KLYDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVE 138
           +L D IF GL++LFDLPLETKMKNVSDKPYHGFLDH APFVLPLH+S GIENA SF+ VE
Sbjct: 61  ELDDAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVE 120

Query: 139 SFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTM 198
           SF+KL+WPSGNHHFCE+LF+Y++M+SEME+LVK+MVFESYGV KYYDSHIASTTYLLR M
Sbjct: 121 SFVKLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGM 180

Query: 199 KYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAG 258
           KYRIP+MGE+NIGAEAHTDKSFFTILHQ NGVNGLEIK K  DHW+ LQF+PNSFLVMAG
Sbjct: 181 KYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIGLQFNPNSFLVMAG 240

Query: 259 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHY 318
           DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHY
Sbjct: 241 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHY 300

Query: 319 GLLGYFSTDECEKMPSTANAYCGV 340
           GLLGYFS ++CEK PSTA AYCGV
Sbjct: 301 GLLGYFSKEDCEKTPSTAGAYCGV 320

[Close]

BLAST of Bhi01G002663 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 262/321 (81.62%), Postives = 289/321 (90.03%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 78
           MG +SS K EVP I FSIEN   L P+STKW+ETCS VRAALEEYGCFLAE E+ + +L 
Sbjct: 1   MGFRSSKKPEVPAINFSIEN---LRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELD 60

Query: 79  DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL 138
           D IF GL++LFDLPLETKMKNVSDKPYHGFLDH APFVLPLH+S GIENA SF+ VESF+
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 139 KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR 198
           KL+WPSGNHHFCE+LF+Y++M+SEME+LVK+MVFESYGV KYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 199 IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC 258
           IP+MGE+NIGAEAHTDKSFFTILHQ NGVNGLEIK K  DHW+ LQF+PNSFLVMAGDAC
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDAC 240

Query: 259 LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLL 318
           LAWSNGRIHSATHRV+IEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLL
Sbjct: 241 LAWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLL 300

Query: 319 GYFSTDECEKMPSTANAYCGV 340
           GYFS +ECEK PSTA AYCGV
Sbjct: 301 GYFSKEECEKTPSTAGAYCGV 316

[Close]

BLAST of Bhi01G002663 vs. NCBI nr
Match: XP_011651543.2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761.1 hypothetical protein Csa_017427 [Cucumis sativus])

HSP 1 Score: 551.6 bits (1420), Expect = 4.7e-153
Identity = 268/338 (79.29%), Postives = 293/338 (86.69%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFS--IENDEDLHPQSTK-WLETCSQVRAALEEYGCFLAEYE---- 78
           MGI   AK EVP+IK S   + D+D  P+S K WLE   QVR ALEEYGCFL EY+    
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 79  ------SASSKL----YDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQ 138
                 S+S KL    Y+EIFKGLKELFDLPLETK+KNVSDKPYHGFLD   PFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 139 SLGIENATSFQAVESFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYY 198
           SLGIENA+S++AV+ F+ LLWPSGNHHFCE LF+YS+ ISEMEQLVK+MVFESYGVGKYY
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFCEKLFAYSKKISEMEQLVKRMVFESYGVGKYY 180

Query: 199 DSHIASTTYLLRTMKYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWL 258
           DSHIASTTYLLRTMKYRIP+MGEENIGAEAHTDKSFFTILHQ +GVNGL+IKPKN D WL
Sbjct: 181 DSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKSFFTILHQFDGVNGLQIKPKNYDQWL 240

Query: 259 RLQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELV 318
            +QFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEG+KERYST LFSYHKGIIEIPQELV
Sbjct: 241 GVQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIEGNKERYSTGLFSYHKGIIEIPQELV 300

Query: 319 DEQHPLRFNPFNHYGLLGYFSTDECEKMPSTANAYCGV 340
           DE+HPLRFNPFNHYGLLGYFSTD+C+K+PSTANAYCGV
Sbjct: 301 DEKHPLRFNPFNHYGLLGYFSTDDCDKIPSTANAYCGV 338

[Close]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL
Match: A0A6J1K6A3 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LOC111492707 PE=3 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 7.9e-154
Identity = 262/321 (81.62%), Postives = 289/321 (90.03%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 78
           MG +SS K EVP I FSIEN   L P+STKW+ETCS VRAALEEYGCFLAE E+ + +L 
Sbjct: 1   MGFRSSKKPEVPAINFSIEN---LRPKSTKWVETCSHVRAALEEYGCFLAECEN-NVELD 60

Query: 79  DEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFL 138
           D IF GL++LFDLPLETKMKNVSDKPYHGFLDH APFVLPLH+S GIENA SF+ VESF+
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 139 KLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYR 198
           KL+WPSGNHHFCE+LF+Y++M+SEME+LVK+MVFESYGV KYYDSHIASTTYLLR MKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 199 IPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDAC 258
           IP+MGE+NIGAEAHTDKSFFTILHQ NGVNGLEIK K  DHW+ LQF+PNSFLVMAGDAC
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDAC 240

Query: 259 LAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLL 318
           LAWSNGRIHSATHRV+IEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHYGLL
Sbjct: 241 LAWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLL 300

Query: 319 GYFSTDECEKMPSTANAYCGV 340
           GYFS +ECEK PSTA AYCGV
Sbjct: 301 GYFSKEECEKTPSTAGAYCGV 316

[Close]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL
Match: A0A6J1GA17 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111452291 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.9e-153
Identity = 261/324 (80.56%), Postives = 289/324 (89.20%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYES---ASS 78
           MG +SS K EVPVI FSIEN   L P+STKW+ETC+ VRAALEEYGCFLAEYE+      
Sbjct: 1   MGFRSSTKPEVPVINFSIEN---LGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEV 60

Query: 79  KLYDEIFKGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVE 138
           +L D IF GL++LFDLPL+TKMKNVSDKPYHGFLDH APFVLPLH+S GIENA SF+ VE
Sbjct: 61  ELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVE 120

Query: 139 SFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTM 198
           SF+KL+WPSGNHHFC++LF+Y++M+SEME+LVK+MVFESYGV KYYDSHIASTTYLLR M
Sbjct: 121 SFVKLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGM 180

Query: 199 KYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAG 258
           KYRIP+MGE+NIGAEAHTDKSFFTILHQ NGVNGLEIK K  DHW+ LQF+ NSFLVMAG
Sbjct: 181 KYRIPEMGEQNIGAEAHTDKSFFTILHQ-NGVNGLEIKAKKDDHWIGLQFNSNSFLVMAG 240

Query: 259 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHY 318
           DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKG+IEIP ELVDEQ PL+FNPFNHY
Sbjct: 241 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHY 300

Query: 319 GLLGYFSTDECEKMPSTANAYCGV 340
           GLLGYFS +ECEK PSTA AYCGV
Sbjct: 301 GLLGYFSKEECEKTPSTAGAYCGV 320

[Close]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL
Match: A0A6J1DH70 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN=LOC111021051 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.2e-147
Identity = 252/317 (79.50%), Postives = 283/317 (89.27%), Query Frame = 0


Query: 23  SSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLYDEIF 82
           S AK+++PVI  SIEN   + P+ST WLE CS +RAALEEYGCFL E+ SA+ +L DEIF
Sbjct: 6   SLAKAKLPVINLSIEN---VSPESTNWLEICSHLRAALEEYGCFLGEFGSATPELDDEIF 65

Query: 83  KGLKELFDLPLETKMKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLW 142
           KGL+ELFDLPLETKMKNVSDKPYHGFLDH  PFVLPLHQS GIEN+ SF+AV+SF+ L+W
Sbjct: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMW 125

Query: 143 PSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQM 202
           PSGN  FCEN+FSY++M+SEME+LVK MVFESYGV KYYDSHIASTTYLLRTMKYRIP+M
Sbjct: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185

Query: 203 GEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWS 262
           GEENIGA+AHTDKSFFTILHQ N VNGLEIK K+G HWL ++F+PNSFLVMAGDACLAWS
Sbjct: 186 GEENIGADAHTDKSFFTILHQ-NEVNGLEIKTKDG-HWLGVEFTPNSFLVMAGDACLAWS 245

Query: 263 NGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFS 322
           NGRIHSATHRV+IEG+KERYSTALFSYHKGIIEIP ELVDE+HPLRF+PFNHYGLL YFS
Sbjct: 246 NGRIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFS 305

Query: 323 TDECEKMPSTANAYCGV 340
           T +  KMPSTA AYCGV
Sbjct: 306 THDSHKMPSTAKAYCGV 317

[Close]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL
Match: A0A1S4DWR1 (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.7e-127
Identity = 213/243 (87.65%), Postives = 231/243 (95.06%), Query Frame = 0


Query: 97  MKNVSDKPYHGFLDHTAPFVLPLHQSLGIENATSFQAVESFLKLLWPSGNHHFCENLFSY 156
           MKNVSDKPYHGFLD  APFVLPLHQSLGIENA+S++AV+SF+ LLWPSGNHHFCENLF+Y
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 157 SEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTMKYRIPQMGEENIGAEAHTDKS 216
           S+ I+EMEQLVK+MVFESYGVGKY DSHIASTTYLLRTMKYRIP+MGEENIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 217 FFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAGDACLAWSNGRIHSATHRVIIE 276
           FFTILHQ +GVNGL+IKPKN D WL + FSPN FLVMAGDACLAWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 277 GSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHYGLLGYFSTDECEKMPSTANAY 336
           G+KERYSTALFSYHKGIIEIPQELVD++HPLRF PFNHYGLLGYFSTD+C+KMPSTANAY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

Query: 337 CGV 340
           CGV
Sbjct: 241 CGV 243

[Close]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL
Match: A0A6P3YXF4 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 GN=LOC107406140 PE=3 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 5.5e-107
Identity = 191/324 (58.95%), Postives = 239/324 (73.77%), Query Frame = 0


Query: 19  MGIQSSAKSEVPVIKFSIENDEDLHPQSTKWLETCSQVRAALEEYGCFLAEYESASSKLY 78
           MG Q  AK  +P I FS EN   L P ++ W  T    R ALE+YGCF+A Y+  S +L+
Sbjct: 1   MGAQPLAK--LPAIDFSKEN---LKPGTSCWSSTAIDARQALEQYGCFVAIYDKMSPQLH 60

Query: 79  DEIFKGLKELFDLPLETKMKNVSDKPYHGFL---DHTAPFVLPLHQSLGIENATSFQAVE 138
           D IF+ LKELFDLP E KMKN+SDKPY G+      T P V  LH+SLGIEN T+ +AV+
Sbjct: 61  DSIFRELKELFDLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTLEAVQ 120

Query: 139 SFLKLLWPSGNHHFCENLFSYSEMISEMEQLVKKMVFESYGVGKYYDSHIASTTYLLRTM 198
           SF  L+WP+GNH FCE + SY+++++E+EQ+VK+MVFESYG  KYYDSH+ STTYLLR +
Sbjct: 121 SFTNLMWPAGNHSFCETVLSYTKLVAELEQMVKRMVFESYGAEKYYDSHVGSTTYLLRQI 180

Query: 199 KYRIPQMGEENIGAEAHTDKSFFTILHQINGVNGLEIKPKNGDHWLRLQFSPNSFLVMAG 258
           KYR P++ E ++G E HTDKSF TILHQ N V G E+K K+G +W+  + SP+SFLV+AG
Sbjct: 181 KYRAPEIDESDVGCEIHTDKSFITILHQ-NEVEGFELKTKDG-YWIGFEPSPSSFLVLAG 240

Query: 259 DACLAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGIIEIPQELVDEQHPLRFNPFNHY 318
           DA LAWSN RIHSA HRV+++GS+ER+S  +FSYH GIIEIP E+VDEQHPL+F  F HY
Sbjct: 241 DAFLAWSNDRIHSAAHRVVMKGSRERFSVGVFSYHNGIIEIPVEVVDEQHPLKFKAFEHY 300

Query: 319 GLLGYFSTDECEKMPSTANAYCGV 340
           GLL YF T    K  STA AYCG+
Sbjct: 301 GLLNYFYTAPGPKTDSTAKAYCGL 317

The following BLAST results are available for this feature:

BLAST of Bhi01G002663 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	1.7e-84	44.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	6.5e-84	47.12	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	5.7e-72	41.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	3.1e-70	42.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	7.0e-70	41.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi01G002663 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTA3	9.8e-69	41.85	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q945B6	1.7e-68	41.98	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q944Z5	1.1e-67	43.51	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	1.2e-63	42.48	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	4.7e-55	30.89	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Bhi01G002663 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878200.1	8.3e-190	100.00	2-oxoglutarate-dependent dioxygenase AOP3-like [Benincasa hispida]	[more]
KAG6606775.1	8.6e-155	81.17	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]
XP_023525673.1	4.3e-154	81.17	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997882.1	1.6e-153	81.62	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima]	[more]
XP_011651543.2	4.7e-153	79.29	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KAE8650761...	[more]

BLAST of Bhi01G002663 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K6A3	7.9e-154	81.62	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GA17	3.9e-153	80.56	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1DH70	7.2e-147	79.50	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Momordica charantia OX=3673 GN...	[more]
A0A1S4DWR1	1.7e-127	87.65	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6P3YXF4	5.5e-107	58.95	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Ziziphus jujuba OX=326968 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 29..108 e-value: 7.9E-11 score: 42.7
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 202..287 e-value: 6.7E-18 score: 65.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..321 e-value: 6.0E-54 score: 185.5
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 22..338
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 22..338
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 27..320
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 187..290 score: 9.012321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M002663	Bhi01M002663	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding