Bhi01G002564 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G002564
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	DNA-directed RNA polymerase subunit
Location	chr1: 83400909 .. 83401278 (-)
RNA-Seq Expression	Bhi01G002564
Synteny	Bhi01G002564

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTCAGGAGAAAATCGGTACAAGAAGCCGTGGATACACTTCTTATGATAATGAATCCACGGAAAATAATGAGGGAGGCCATAATATGATTTACAAGTCGTTTTCCGATGTAATGAAGCAAAGAGGGAAGATTTCGGAGACTCTGCTTGGCAAACGGGTCGATTATTCAGGACGTTTTGTGATTGTTGTAGGTCCTCACTTTCATTACATCGATGTGGATTGCCTCGCGAAATCGCAATAGAGCTTTTCCAGACATTTTTAATTCGTGGTCTAATTCGACAACAATTTTGCTTCGAACATAGAAGTTGCTAAGAGTAAAATTCAGGAAAAAGAACCCATTGTATGGGAAATACTTCAGGAAGTTATGA

mRNA sequence

ATGTGTCAGGAGAAAATCGGTACAAGAAGCCGTGGATACACTTCTTATGATAATGAATCCACGGAAAATAATGAGGGAGGCCATAATATGATTTACAAGTCGTTTTCCGATGTAATGAAGCAAAGAGGGAAGATTTCGGAGACTCTGCTTGGCAAACGGGTCGATTATTCAGGACGTTTTGTGATTGTTAAGTTGCTAAGAGTAAAATTCAGGAAAAAGAACCCATTGTATGGGAAATACTTCAGGAAGTTATGA

Coding sequence (CDS)

ATGTGTCAGGAGAAAATCGGTACAAGAAGCCGTGGATACACTTCTTATGATAATGAATCCACGGAAAATAATGAGGGAGGCCATAATATGATTTACAAGTCGTTTTCCGATGTAATGAAGCAAAGAGGGAAGATTTCGGAGACTCTGCTTGGCAAACGGGTCGATTATTCAGGACGTTTTGTGATTGTTAAGTTGCTAAGAGTAAAATTCAGGAAAAAGAACCCATTGTATGGGAAATACTTCAGGAAGTTATGA

Protein sequence

MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMKQRGKISETLLGKRVDYSGRFVIVKLLRVKFRKKNPLYGKYFRKL

Homology

[Close]

BLAST of Bhi01G002564 vs. TAIR 10
Match: ATCG00180.1 (DNA-directed RNA polymerase family protein )

HSP 1 Score: 56.2 bits (134), Expect = 1.4e-08
Identity = 32/64 (50.00%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 322 MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 381

Query: 61  FVIV 64
            VIV
Sbjct: 382 SVIV 385

[Close]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot
Match: Q3BAQ1 (DNA-directed RNA polymerase subunit beta' OS=Phalaenopsis aphrodite subsp. formosana OX=308872 GN=rpoC1 PE=3 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 7.0e-08
Identity = 35/65 (53.85%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNE-STENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSG 60
           MCQEK+  +    T +DN    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSG
Sbjct: 322 MCQEKL-VQEAVDTLFDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSG 381

Query: 61  RFVIV 64
           R VIV
Sbjct: 382 RSVIV 385

[Close]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot
Match: Q09X26 (DNA-directed RNA polymerase subunit beta' OS=Morus indica OX=248361 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.1e-08
Identity = 34/65 (52.31%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNE-STENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSG 60
           MCQEK+  +    T +DN    +    GHN +YKSFSD+++ + G+  ETLLGKRVDYSG
Sbjct: 322 MCQEKL-VQEAVDTLFDNGIRGQPMRDGHNKVYKSFSDIIEGKEGRFRETLLGKRVDYSG 381

Query: 61  RFVIV 64
           R VIV
Sbjct: 382 RSVIV 385

[Close]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot
Match: A1XGM9 (DNA-directed RNA polymerase subunit beta' OS=Ranunculus macranthus OX=334596 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.1e-08
Identity = 33/64 (51.56%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN IYKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 322 MCQEKLVQEAVDTLLDNGIRGQPTRDGHNKIYKSFSDVIEGKEGRFRETLLGKRVDYSGR 381

Query: 61  FVIV 64
            VIV
Sbjct: 382 SVIV 385

[Close]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot
Match: A4QJJ0 (DNA-directed RNA polymerase subunit beta' OS=Aethionema grandiflorum OX=72657 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-07
Identity = 32/64 (50.00%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +  + GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 322 MCQEKLVQEAVDTLLDNGIRGQPMKDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 381

Query: 61  FVIV 64
            VIV
Sbjct: 382 SVIV 385

[Close]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot
Match: A6MMB3 (DNA-directed RNA polymerase subunit beta' OS=Chloranthus spicatus OX=13006 GN=rpoC1 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 1.6e-07
Identity = 32/64 (50.00%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +  + GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 326 MCQEKLVQEAVDTLLDNGIRGQPMKDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 385

Query: 61  FVIV 64
            VIV
Sbjct: 386 SVIV 389

[Close]

BLAST of Bhi01G002564 vs. NCBI nr
Match: ERN02619.1 (hypothetical protein AMTR_s00390p00014170, partial [Amborella trichopoda])

HSP 1 Score: 87.4 bits (215), Expect = 6.3e-14
Identity = 49/85 (57.65%), Postives = 60/85 (70.59%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 173 MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 232

Query: 61  FVIVKLLRVKFRKKNPLYGKYFRKL 85
            VIV+LLRVKF KK+ LYGKYFRKL
Sbjct: 233 SVIVELLRVKFEKKSRLYGKYFRKL 257

[Close]

BLAST of Bhi01G002564 vs. NCBI nr
Match: CAB4289788.1 (unnamed protein product [Prunus armeniaca] >CAB4299885.1 unnamed protein product [Prunus armeniaca])

HSP 1 Score: 70.9 bits (172), Expect = 6.1e-09
Identity = 48/109 (44.04%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 59  MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 118

Query: 61  FVIV------------------------KLLRVKFRKKNPLYGKYFRKL 85
            VIV                        +LLRVKF KK+ LYGKYF KL
Sbjct: 119 SVIVVGPSLSLHRCGLPREIAIELFQTFELLRVKFGKKSRLYGKYFMKL 167

[Close]

BLAST of Bhi01G002564 vs. NCBI nr
Match: VVW91431.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 64.3 bits (155), Expect = 5.7e-07
Identity = 46/123 (37.40%), Postives = 58/123 (47.15%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 1   MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 60

Query: 61  FVIV--------------------------------------KLLRVKFRKKNPLYGKYF 85
            VIV                                       L + K R+KNPLYGKYF
Sbjct: 61  SVIVVGPSLSLHQCGLPREIAIELFQTFVIRGLIRQHLASNIGLAKSKIREKNPLYGKYF 120

[Close]

BLAST of Bhi01G002564 vs. NCBI nr
Match: SBN37586.1 (DNA dependent RNA polymerase, beta subunit, partial [Sambucus sp. 1 JA-2016])

HSP 1 Score: 63.5 bits (153), Expect = 9.7e-07
Identity = 31/42 (73.81%), Postives = 36/42 (85.71%), Query Frame = 0


Query: 23 NNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGRFVIV 64
          NNEGGHN +YKSFSDV++ + G+  ETLLGKRVDYSGR VIV
Sbjct: 1  NNEGGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIV 42

[Close]

BLAST of Bhi01G002564 vs. NCBI nr
Match: VVW90806.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 63.5 bits (153), Expect = 9.7e-07
Identity = 41/97 (42.27%), Postives = 49/97 (50.52%), Query Frame = 0


Query: 27  GHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGRFVIV---------------------- 85
           GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR VIV                      
Sbjct: 27  GHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHQCGLPREIAIELFQ 86

[Close]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL
Match: W1P4N9 (DNA-directed RNA polymerase subunit (Fragment) OS=Amborella trichopoda OX=13333 GN=AMTR_s00390p00014170 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.0e-14
Identity = 49/85 (57.65%), Postives = 60/85 (70.59%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 173 MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 232

Query: 61  FVIVKLLRVKFRKKNPLYGKYFRKL 85
            VIV+LLRVKF KK+ LYGKYFRKL
Sbjct: 233 SVIVELLRVKFEKKSRLYGKYFRKL 257

[Close]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL
Match: A0A6J5VV95 (DNA-directed RNA polymerase subunit OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS49370 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.9e-09
Identity = 48/109 (44.04%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 59  MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 118

Query: 61  FVIV------------------------KLLRVKFRKKNPLYGKYFRKL 85
            VIV                        +LLRVKF KK+ LYGKYF KL
Sbjct: 119 SVIVVGPSLSLHRCGLPREIAIELFQTFELLRVKFGKKSRLYGKYFMKL 167

[Close]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL
Match: A0A5K1HQG7 (DNA-directed RNA polymerase subunit (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS30932 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.8e-07
Identity = 46/123 (37.40%), Postives = 58/123 (47.15%), Query Frame = 0


Query: 1   MCQEKIGTRSRGYTSYDNESTENNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGR 60
           MCQEK+   +      +    +    GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR
Sbjct: 1   MCQEKLVQEAVDTLLDNGIRGQPMRDGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGR 60

Query: 61  FVIV--------------------------------------KLLRVKFRKKNPLYGKYF 85
            VIV                                       L + K R+KNPLYGKYF
Sbjct: 61  SVIVVGPSLSLHQCGLPREIAIELFQTFVIRGLIRQHLASNIGLAKSKIREKNPLYGKYF 120

[Close]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL
Match: A0A181Z6X1 (DNA-directed RNA polymerase (Fragment) OS=Sambucus sp. 1 JA-2016 OX=1851736 GN=rpoC1 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.7e-07
Identity = 31/42 (73.81%), Postives = 36/42 (85.71%), Query Frame = 0


Query: 23 NNEGGHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGRFVIV 64
          NNEGGHN +YKSFSDV++ + G+  ETLLGKRVDYSGR VIV
Sbjct: 1  NNEGGHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIV 42

[Close]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL
Match: A0A5K1HS53 (DNA-directed RNA polymerase subunit (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS30763 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.7e-07
Identity = 41/97 (42.27%), Postives = 49/97 (50.52%), Query Frame = 0


Query: 27  GHNMIYKSFSDVMK-QRGKISETLLGKRVDYSGRFVIV---------------------- 85
           GHN +YKSFSDV++ + G+  ETLLGKRVDYSGR VIV                      
Sbjct: 27  GHNKVYKSFSDVIEGKEGRFRETLLGKRVDYSGRSVIVVGPSLSLHQCGLPREIAIELFQ 86

The following BLAST results are available for this feature:

BLAST of Bhi01G002564 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00180.1	1.4e-08	50.00	DNA-directed RNA polymerase family protein	[more]

BLAST of Bhi01G002564 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3BAQ1	7.0e-08	53.85	DNA-directed RNA polymerase subunit beta' OS=Phalaenopsis aphrodite subsp. formo...	[more]
Q09X26	9.1e-08	52.31	DNA-directed RNA polymerase subunit beta' OS=Morus indica OX=248361 GN=rpoC1 PE=...	[more]
A1XGM9	9.1e-08	51.56	DNA-directed RNA polymerase subunit beta' OS=Ranunculus macranthus OX=334596 GN=...	[more]
A4QJJ0	1.6e-07	50.00	DNA-directed RNA polymerase subunit beta' OS=Aethionema grandiflorum OX=72657 GN...	[more]
A6MMB3	1.6e-07	50.00	DNA-directed RNA polymerase subunit beta' OS=Chloranthus spicatus OX=13006 GN=rp...	[more]

BLAST of Bhi01G002564 vs. NCBI nr


Match Name	E-value	Identity	Description
ERN02619.1	6.3e-14	57.65	hypothetical protein AMTR_s00390p00014170, partial [Amborella trichopoda]	[more]
CAB4289788.1	6.1e-09	44.04	unnamed protein product [Prunus armeniaca] >CAB4299885.1 unnamed protein product...	[more]
VVW91431.1	5.7e-07	37.40	unnamed protein product, partial [Nymphaea colorata]	[more]
SBN37586.1	9.7e-07	73.81	DNA dependent RNA polymerase, beta subunit, partial [Sambucus sp. 1 JA-2016]	[more]
VVW90806.1	9.7e-07	42.27	unnamed protein product, partial [Nymphaea colorata]	[more]

BLAST of Bhi01G002564 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
W1P4N9	3.0e-14	57.65	DNA-directed RNA polymerase subunit (Fragment) OS=Amborella trichopoda OX=13333 ...	[more]
A0A6J5VV95	2.9e-09	44.04	DNA-directed RNA polymerase subunit OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS...	[more]
A0A5K1HQG7	2.8e-07	37.40	DNA-directed RNA polymerase subunit (Fragment) OS=Nymphaea colorata OX=210225 GN...	[more]
A0A181Z6X1	4.7e-07	73.81	DNA-directed RNA polymerase (Fragment) OS=Sambucus sp. 1 JA-2016 OX=1851736 GN=r...	[more]
A0A5K1HS53	4.7e-07	42.27	DNA-directed RNA polymerase subunit (Fragment) OS=Nymphaea colorata OX=210225 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..28
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 14..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M002564	Bhi01M002564	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0046872	metal ion binding