Bhi01G002517 (gene) Wax gourd (B227) v1

Overview
NameBhi01G002517
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionKazal-type serine protease inhibitor
Locationchr1: 81046337 .. 81046729 (-)
RNA-Seq ExpressionBhi01G002517
SyntenyBhi01G002517
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCCTCTTCTCAAGACCCCCGATTTCCATCGTTTTCTTCTTTTCGCTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATGTTTCTTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACGACGGCGATGTAGATCTCTGTCCTGTATCGGTCCCCTCTTCTTGCCCCGTGAAATGCTTTAGGACGGACCCGGTCTGCGGGGTTGATGGCCTTACCTACTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGAAACGGGGGTTCTGCACCTATTCCCGGCCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

mRNA sequence

ATGTTCCTCTTCTCAAGACCCCCGATTTCCATCGTTTTCTTCTTTTCGCTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATGTTTCTTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACGACGGCGATGTAGATCTCTGTCCTGTATCGGTCCCCTCTTCTTGCCCCGTGAAATGCTTTAGGACGGACCCGGTCTGCGGGGTTGATGGCCTTACCTACTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGAAACGGGGGTTCTGCACCTATTCCCGGCCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

Coding sequence (CDS)

ATGTTCCTCTTCTCAAGACCCCCGATTTCCATCGTTTTCTTCTTTTCGCTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATGTTTCTTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACGACGGCGATGTAGATCTCTGTCCTGTATCGGTCCCCTCTTCTTGCCCCGTGAAATGCTTTAGGACGGACCCGGTCTGCGGGGTTGATGGCCTTACCTACTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGAAACGGGGGTTCTGCACCTATTCCCGGCCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

Protein sequence

MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVKCFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLIILGVSVLFGLF
Homology
BLAST of Bhi01G002517 vs. TAIR 10
Match: AT4G01575.1 (serine protease inhibitor, Kazal-type family protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.0e-32
Identity = 68/130 (52.31%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 7   PPISIVFFFSLLIF----VFAVRSEDVSSAIRLPSEATNNDGD-VDLCP-VSVPSSCPVK 66
           P ++++ F  L++     VFA  S +    IRLPSE  N + +  + C  ++ P+SCPV+
Sbjct: 15  PSLAVIAFLFLILLNLSSVFADPSTEGGEIIRLPSEKINGEKNRGEFCEGIAKPASCPVQ 74

Query: 67  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 126
           CFR DPVCG D +TYWCGCADALC GV+V K G C+VGNG    +PGQALLL+HI+W+++
Sbjct: 75  CFRPDPVCGEDSVTYWCGCADALCHGVRVVKQGACDVGNGVGLSVPGQALLLIHIVWMML 134

Query: 127 LGVSVLFGLF 131
           LG S+LFGLF
Sbjct: 135 LGFSILFGLF 144

BLAST of Bhi01G002517 vs. TAIR 10
Match: AT3G61980.1 (serine protease inhibitor, Kazal-type family protein )

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-25
Identity = 60/123 (48.78%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 9   ISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCP-VSVPSSCPVKCFRTDPV 68
           +SI F F +L  +    ++D     R         GDV  CP V     C + CFR DPV
Sbjct: 6   LSIRFLFLVLCLIGLQAADDFPDKSR---------GDV--CPRVKDRGGCTINCFRADPV 65

Query: 69  CGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLIILGVSVLF 128
           CG DG+TYWCGC DA C G +V K G C+ GN GSA +PGQALLL+HI+WL +LG+S+L 
Sbjct: 66  CGTDGVTYWCGCPDAACHGARVVKKGACDTGNAGSASVPGQALLLIHIVWLFLLGLSLLV 117

Query: 129 GLF 131
           G F
Sbjct: 126 GGF 117

BLAST of Bhi01G002517 vs. NCBI nr
Match: XP_038892451.1 (uncharacterized protein LOC120081544 [Benincasa hispida])

HSP 1 Score: 260.8 bits (665), Expect = 6.4e-66
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. NCBI nr
Match: XP_016902950.1 (PREDICTED: uncharacterized protein LOC107991954 [Cucumis melo] >KAA0056649.1 uncharacterized protein E6C27_scaffold288G001800 [Cucumis melo var. makuwa] >TYK04610.1 uncharacterized protein E5676_scaffold351G00140 [Cucumis melo var. makuwa])

HSP 1 Score: 250.8 bits (639), Expect = 6.6e-63
Identity = 124/130 (95.38%), Postives = 126/130 (96.92%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PP+SIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. NCBI nr
Match: XP_004140750.1 (uncharacterized protein LOC101210178 [Cucumis sativus])

HSP 1 Score: 246.5 bits (628), Expect = 1.3e-61
Identity = 123/130 (94.62%), Postives = 125/130 (96.15%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFSRP ISI+F F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. NCBI nr
Match: KAA0033716.1 (Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 244.6 bits (623), Expect = 4.8e-61
Identity = 123/130 (94.62%), Postives = 124/130 (95.38%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PPISIVF F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSTPPISIVFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSA IPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. NCBI nr
Match: TYK23009.1 (Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 244.2 bits (622), Expect = 6.2e-61
Identity = 122/130 (93.85%), Postives = 124/130 (95.38%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PPISI+F F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSTPPISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSA IPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. ExPASy TrEMBL
Match: A0A5A7UT13 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold351G00140 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.2e-63
Identity = 124/130 (95.38%), Postives = 126/130 (96.92%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PP+SIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. ExPASy TrEMBL
Match: A0A1S4E4Q0 (uncharacterized protein LOC107991954 OS=Cucumis melo OX=3656 GN=LOC107991954 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.2e-63
Identity = 124/130 (95.38%), Postives = 126/130 (96.92%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PP+SIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. ExPASy TrEMBL
Match: A0A0A0L616 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184020 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.1e-62
Identity = 123/130 (94.62%), Postives = 125/130 (96.15%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFSRP ISI+F F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. ExPASy TrEMBL
Match: A0A5A7SUT2 (Serine-type endopeptidase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001890 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.3e-61
Identity = 123/130 (94.62%), Postives = 124/130 (95.38%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PPISIVF F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSTPPISIVFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSA IPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of Bhi01G002517 vs. ExPASy TrEMBL
Match: A0A5D3DIC6 (Serine-type endopeptidase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001170 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.0e-61
Identity = 122/130 (93.85%), Postives = 124/130 (95.38%), Query Frame = 0

Query: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60
           M LFS PPISI+F F LLIFVF VRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK
Sbjct: 1   MTLFSTPPISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSA IPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G01575.11.0e-3252.31serine protease inhibitor, Kazal-type family protein [more]
AT3G61980.11.5e-2548.78serine protease inhibitor, Kazal-type family protein [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038892451.16.4e-66100.00uncharacterized protein LOC120081544 [Benincasa hispida][more]
XP_016902950.16.6e-6395.38PREDICTED: uncharacterized protein LOC107991954 [Cucumis melo] >KAA0056649.1 unc... [more]
XP_004140750.11.3e-6194.62uncharacterized protein LOC101210178 [Cucumis sativus][more]
KAA0033716.14.8e-6194.62Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa][more]
TYK23009.16.2e-6193.85Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A5A7UT133.2e-6395.38Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S4E4Q03.2e-6395.38uncharacterized protein LOC107991954 OS=Cucumis melo OX=3656 GN=LOC107991954 PE=... [more]
A0A0A0L6166.1e-6294.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184020 PE=4 SV=1[more]
A0A5A7SUT22.3e-6194.62Serine-type endopeptidase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3DIC63.0e-6193.85Serine-type endopeptidase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34376SERINE PROTEASE INHIBITOR, KAZAL-TYPE FAMILY PROTEINcoord: 9..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M002517Bhi01M002517mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane