Bhi01G002274 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G002274
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Phytocyanin domain-containing protein
Location	chr1: 72268121 .. 72269269 (-)
RNA-Seq Expression	Bhi01G002274
Synteny	Bhi01G002274

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAATCCCATTGCCTATTTCTCTTCTTTTTGTTCTCTTTGGAATTGGAGGAGAAAGAAGAAAGAAGATTCATTTACAAAACAGAAAAGAAAAACCTTTTTCTCTCCCCCTCTCTTTTATTTTTCTGATCTCTCTGTTTTAAGCTCCAAAAGAGAGATTGGCCAAATTAAAGGGAAAACCAAATTAAAAAAAAAAATGGACAAAACAGTAATGATTTGTTGCTTAGTGATGATGATGATGGGATCATGTGTTAATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAACATTACCTTTTTTGATGAATGGACTAAACCAAGAACCTTTGGTGTTGGAGACAGGCTTGGTATCTCTTTTTCTTTTCTTTTTCCTCACTTTTTACCTTTTTTTTTAAATCCATTTTACAATTTTACCCTTTCTCACGTTAAAGTGTAAATTTGATCTTCTTTCGATAAAATTCTAGACTATTTCTGAAGTTTTATCAAATCAAAAAGACTAAAAACATTGTATAAAACAGTTCTTCGAATTGTGTCTGTCGATGAGGTTGACATTGACACACACAACCCTGAACTATATTTCATTACGTTTCTAACGTTTGGGTGGTTTTATTTTTCAATTGGATACGTACACAGTATTCCCGTACCGGCCAGGTGCCAACAACCTATTGGCTGTGAAGAAGGCAGACTATGATACATGTGGAGAAGAAGCAGTAATACACATGTATTTTCTAGGGCCAACAGTCTTGAACTTAACTGAGCCAGGTGATTACTACTACTTTGATGGAATTGGAAAGCATTGTGAAGCTGGTCAAAAGCTCCATATCCAAGTTGGCTCAAAAGAAGGAACCTCTGGCTCTGACCCTATGCCATTTAACCTTGAAACTTTTGGAATCCACACCAATTTGGGCCCAGCCATGGCACCCACAGCCCAAACCCAAGCCGCAGCCAATGCCACTCACCCATCCAATGCCTTCCTTCTTCCTCCACCAATATTGCCTCCTATAATTGCCTTCCTTTTCTCCATATTTCTTTGATTTCCATATATTATCCCTTTGCCTTTGCTTTTTAATGTGTATCATTGTTCCAAATTCATCTTTTCTTGTGTTTCCAATCTTCAAAATCAACTATAAAC

mRNA sequence

CTAAATCCCATTGCCTATTTCTCTTCTTTTTGTTCTCTTTGGAATTGGAGGAGAAAGAAGAAAGAAGATTCATTTACAAAACAGAAAAGAAAAACCTTTTTCTCTCCCCCTCTCTTTTATTTTTCTGATCTCTCTGTTTTAAGCTCCAAAAGAGAGATTGGCCAAATTAAAGGGAAAACCAAATTAAAAAAAAAAATGGACAAAACAGTAATGATTTGTTGCTTAGTGATGATGATGATGGGATCATGTGTTAATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAACATTACCTTTTTTGATGAATGGACTAAACCAAGAACCTTTGGTGTTGGAGACAGGCTTGTATTCCCGTACCGGCCAGGTGCCAACAACCTATTGGCTGTGAAGAAGGCAGACTATGATACATGTGGAGAAGAAGCAGTAATACACATGTATTTTCTAGGGCCAACAGTCTTGAACTTAACTGAGCCAGGTGATTACTACTACTTTGATGGAATTGGAAAGCATTGTGAAGCTGGTCAAAAGCTCCATATCCAAGTTGGCTCAAAAGAAGGAACCTCTGGCTCTGACCCTATGCCATTTAACCTTGAAACTTTTGGAATCCACACCAATTTGGGCCCAGCCATGGCACCCACAGCCCAAACCCAAGCCGCAGCCAATGCCACTCACCCATCCAATGCCTTCCTTCTTCCTCCACCAATATTGCCTCCTATAATTGCCTTCCTTTTCTCCATATTTCTTTGATTTCCATATATTATCCCTTTGCCTTTGCTTTTTAATGTGTATCATTGTTCCAAATTCATCTTTTCTTGTGTTTCCAATCTTCAAAATCAACTATAAAC

Coding sequence (CDS)

ATGGACAAAACAGTAATGATTTGTTGCTTAGTGATGATGATGATGGGATCATGTGTTAATGCATTCACTCATATTGTTGGTGGAAGCCATGGTTGGAGAGTCCCTGAAAACATTACCTTTTTTGATGAATGGACTAAACCAAGAACCTTTGGTGTTGGAGACAGGCTTGTATTCCCGTACCGGCCAGGTGCCAACAACCTATTGGCTGTGAAGAAGGCAGACTATGATACATGTGGAGAAGAAGCAGTAATACACATGTATTTTCTAGGGCCAACAGTCTTGAACTTAACTGAGCCAGGTGATTACTACTACTTTGATGGAATTGGAAAGCATTGTGAAGCTGGTCAAAAGCTCCATATCCAAGTTGGCTCAAAAGAAGGAACCTCTGGCTCTGACCCTATGCCATTTAACCTTGAAACTTTTGGAATCCACACCAATTTGGGCCCAGCCATGGCACCCACAGCCCAAACCCAAGCCGCAGCCAATGCCACTCACCCATCCAATGCCTTCCTTCTTCCTCCACCAATATTGCCTCCTATAATTGCCTTCCTTTTCTCCATATTTCTTTGA

Protein sequence

MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPIIAFLFSIFL

Homology

[Close]

BLAST of Bhi01G002274 vs. TAIR 10
Match: AT3G18590.1 (early nodulin-like protein 5 )

HSP 1 Score: 81.6 bits (200), Expect = 7.2e-16
Identity = 52/161 (32.30%), Postives = 80/161 (49.69%), Query Frame = 0


Query: 3   KTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITF---FDEWTKPRTFGVGDRLVFP 62
           K +++  LV   M SCV++    VGG +GW VP++ T    F++W     F VGD L F 
Sbjct: 6   KIIIVMFLVTFYMFSCVSSTEFEVGGENGWIVPKSKTLGDAFNQWASDNRFKVGDTLRFK 65

Query: 63  YRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLH 122
           Y    +++L V + +Y  C             TV  L  PG +Y+  G+  HCE GQK+ 
Sbjct: 66  YT--KDSVLVVSEEEYKKCKATKPQLYSNNEDTVFKLDRPGLFYFISGVSGHCEKGQKMI 125

Query: 123 IQVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAA 161
           ++V   E ++ S P P +  +    ++L PA  P A+   A
Sbjct: 126 VKVMETESSTESPP-PSSSSSSSSSSSL-PASTPKAKKSNA 162

[Close]

BLAST of Bhi01G002274 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-14
Identity = 38/99 (38.38%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 24  HIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKADYDTCGEEAV 83
           HIVG S+GW   E  T +  WT+ R F VGD LVF Y+   +N++ V    Y  CG +  
Sbjct: 9   HIVGDSNGW---ELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDNY 68

Query: 84  IHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQV 123
             ++  G   + L+E G  ++  G+  HC  GQKL I V
Sbjct: 69  TTLFTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSINV 104

[Close]

BLAST of Bhi01G002274 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.3e-14
Identity = 44/146 (30.14%), Postives = 65/146 (44.52%), Query Frame = 0


Query: 10  LVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLA 69
           LV++     V A  + VG    W  P +  F+  W   +TF VGD L F +  G +++  
Sbjct: 11  LVLVFAAVVVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGRHDVAV 70

Query: 70  VKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTS 129
           V +A ++ C +E  I    + P  + L   G  Y+   +G HC  GQKL I V +   T 
Sbjct: 71  VSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATG 130

Query: 130 GSDPMPFNLETFGIHTNLGPAMAPTA 156
           G+ P        G   + G    PTA
Sbjct: 131 GATPGAGATPAPGSTPSTGGTTPPTA 156

[Close]

BLAST of Bhi01G002274 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 76.3 bits (186), Expect = 3.0e-14
Identity = 50/189 (26.46%), Postives = 87/189 (46.03%), Query Frame = 0


Query: 5   VMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGA 64
           ++I  L   ++G  V A  H +GG  GW V  ++     W   +TF VGD LVF Y    
Sbjct: 8   IIISVLATTLIGLTV-ATDHTIGGPSGWTVGASLR---TWAAGQTFAVGDNLVFSYPAAF 67

Query: 65  NNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGS 124
           ++++ V K ++D+C     +  +  G +++ LT PG  Y+  G+  HC  G KL + V  
Sbjct: 68  HDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVP 127

Query: 125 KEGTSGSDPMPFNLETFG-------------IHTNLGPAMAPTAQTQAAANATHPSNAFL 181
               + + P+P  + +               +  N  P ++P++ T        PS++  
Sbjct: 128 TATVAPTAPLPNTVPSLNAPSPSSVLPIQPLLPLNPVPVLSPSSSTPL------PSSSLP 186

[Close]

BLAST of Bhi01G002274 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.6e-13
Identity = 52/177 (29.38%), Postives = 78/177 (44.07%), Query Frame = 0


Query: 7   ICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANN 66
           + C+V+M+  S   A  + VG S GW    N+  +  W   +TF +GD ++F Y P  +N
Sbjct: 9   LACIVVMLRLS--EAAVYKVGDSAGWTTIANVD-YKLWASTKTFHIGDTVLFEYNPQFHN 68

Query: 67  LLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQV-GSK 126
           ++ V    Y +C     I  +  G   + LT  G +++F G+  HC AGQKL + V    
Sbjct: 69  VMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHVLLPA 128

Query: 127 EGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPIIA 183
             T  SDP           T+   +   T    A      PS A  LP  +   I+A
Sbjct: 129 SSTPLSDP----------PTSSSSSPPSTTIPAAGVPGPSPSLAASLPSMVTAQIVA 172

[Close]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 0


Query: 24  HIVGGSHGWRVP--ENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKADYDTCGEE 83
           + VG S GW+VP   ++ +  +W   +TF +GD LVF Y    +N+  V + +Y +C + 
Sbjct: 3   YTVGDSAGWKVPFFGDVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCNDT 62

Query: 84  AVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQV 123
             I  Y  G   +NL   G  YY  G+ KHC+ GQK+HI V
Sbjct: 63  TPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHINV 103

[Close]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.9e-13
Identity = 37/105 (35.24%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 26  VGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKADYDTCGEEAVIH 85
           VGG   W+ P +  F+  W   +TF VGD L F +  G +++  V K  +D C +E  I 
Sbjct: 5   VGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPIS 64

Query: 86  MYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTSG 131
                P  + L   G  YY   +G HC  GQKL I V    G  G
Sbjct: 65  HMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGG 109

[Close]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 44/146 (30.14%), Postives = 65/146 (44.52%), Query Frame = 0


Query: 10  LVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLA 69
           LV++     V A  + VG    W  P +  F+  W   +TF VGD L F +  G +++  
Sbjct: 11  LVLVFAAVVVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFDFAAGRHDVAV 70

Query: 70  VKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTS 129
           V +A ++ C +E  I    + P  + L   G  Y+   +G HC  GQKL I V +   T 
Sbjct: 71  VSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLSITVVAAGATG 130

Query: 130 GSDPMPFNLETFGIHTNLGPAMAPTA 156
           G+ P        G   + G    PTA
Sbjct: 131 GATPGAGATPAPGSTPSTGGTTPPTA 156

[Close]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.3e-13
Identity = 50/189 (26.46%), Postives = 87/189 (46.03%), Query Frame = 0


Query: 5   VMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGA 64
           ++I  L   ++G  V A  H +GG  GW V  ++     W   +TF VGD LVF Y    
Sbjct: 8   IIISVLATTLIGLTV-ATDHTIGGPSGWTVGASLR---TWAAGQTFAVGDNLVFSYPAAF 67

Query: 65  NNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGS 124
           ++++ V K ++D+C     +  +  G +++ LT PG  Y+  G+  HC  G KL + V  
Sbjct: 68  HDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVP 127

Query: 125 KEGTSGSDPMPFNLETFG-------------IHTNLGPAMAPTAQTQAAANATHPSNAFL 181
               + + P+P  + +               +  N  P ++P++ T        PS++  
Sbjct: 128 TATVAPTAPLPNTVPSLNAPSPSSVLPIQPLLPLNPVPVLSPSSSTPL------PSSSLP 186

[Close]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 50/182 (27.47%), Postives = 83/182 (45.60%), Query Frame = 0


Query: 7   ICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANN 66
           +  ++ M + S    +T  VG + GW +  +   +  W   +TF VGD LVF Y  GA+ 
Sbjct: 12  LLAIINMALPSLATVYT--VGDTSGWVIGGD---YSTWASDKTFAVGDSLVFNYGAGAHT 71

Query: 67  LLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKE 126
           +  VK++DY +C     I     G T + L + G +Y+  G+  H   G KL I+V +  
Sbjct: 72  VDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASS 131

Query: 127 GTSGS-DPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPIIAFLF 186
           G+S +    P +       ++  PA   T  T    N +  ++        L PI+A  F
Sbjct: 132 GSSAAPSATPSSSGKGSPSSDDTPAATTTTTTPTKQNESSATS--------LSPIVALFF 180

Query: 187 SI 188
           ++
Sbjct: 192 TV 180

[Close]

BLAST of Bhi01G002274 vs. NCBI nr
Match: XP_038878156.1 (stellacyanin-like [Benincasa hispida])

HSP 1 Score: 398.7 bits (1023), Expect = 2.9e-107
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0


Query: 1   MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY 60
           MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY
Sbjct: 1   MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY 60

Query: 61  RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI 120
           RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI
Sbjct: 61  RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI 120

Query: 121 QVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPI 180
           QVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPI
Sbjct: 121 QVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPSNAFLLPPPILPPI 180

Query: 181 IAFLFSIFL 190
           IAFLFSIFL
Sbjct: 181 IAFLFSIFL 189

[Close]

BLAST of Bhi01G002274 vs. NCBI nr
Match: XP_004149757.2 (blue copper protein [Cucumis sativus] >KGN53385.1 hypothetical protein Csa_014752 [Cucumis sativus])

HSP 1 Score: 302.0 bits (772), Expect = 3.7e-78
Identity = 149/204 (73.04%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MDKTVMICC----LVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRL 60
           MDKT+M  C    ++MM+MGSCV+AFTHIVGGSHGWRVPEN +FFD+W KPRTFGVGDRL
Sbjct: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60

Query: 61  VFPYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQ 120
           VFPYR GANNL+ VKKADYDTCGEE VI+MYFLGPTV+NLT+ GDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQA---------AANATHPS 180
           KLHIQVG+KEG+SGSDP+PFNLETFGIHTNLGPA++P  Q  A         +    HPS
Sbjct: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180

Query: 181 NAFLL--PPPILPPIIAFLFSIFL 190
           NAFLL   P +L  II  LFSIFL
Sbjct: 181 NAFLLLPTPMLLALIIPTLFSIFL 204

[Close]

BLAST of Bhi01G002274 vs. NCBI nr
Match: XP_022985421.1 (stellacyanin-like [Cucurbita maxima])

HSP 1 Score: 286.6 bits (732), Expect = 1.6e-73
Identity = 141/198 (71.21%), Postives = 162/198 (81.82%), Query Frame = 0


Query: 1   MDKTVMICCL--VMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVF 60
           MDK +MI  +  +MMMMGS VNAFTHIVG SHGWRVP+N+TF+DEW KPRTFGVGD+LVF
Sbjct: 1   MDKFMMIMTITTMMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVF 60

Query: 61  PYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKL 120
           PYRPGANN++AVKK DY+ CGEE VI+MY+LGPT+LNLTE GDYYYFDGIGKHCEAGQKL
Sbjct: 61  PYRPGANNIVAVKKTDYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKL 120

Query: 121 HIQVGSKEGTSGSDPMPFNLETFGIHTNL------GPAMAPTAQTQAA-ANATHPSNAFL 180
           H+QVGSKEGTSG+DP+PFNLETFGIHTNL      GPA APTA T+ + AN    +NA L
Sbjct: 121 HVQVGSKEGTSGADPLPFNLETFGIHTNLDPTQNVGPAAAPTAATKGSNANVNANANALL 180

Query: 181 LPPPILPPIIAFLFSIFL 190
           +P  +    IA  FS FL
Sbjct: 181 VPAGL--AAIAMFFSTFL 196

[Close]

BLAST of Bhi01G002274 vs. NCBI nr
Match: XP_023552874.1 (stellacyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.9 bits (712), Expect = 3.3e-71
Identity = 132/181 (72.93%), Postives = 153/181 (84.53%), Query Frame = 0


Query: 12  MMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVK 71
           MMMMGS VNAFTHIVG SHGWRVP+N+TF+DEW KPRTFGVGD+LVFPYRPGANN++AVK
Sbjct: 1   MMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNIVAVK 60

Query: 72  KADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTSGS 131
           KADY+ CGEE VI+MY+LGPT+LNLTE GDYYYFDGIGKHCEAGQKLH+QVGSK+GTSG+
Sbjct: 61  KADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHVQVGSKDGTSGA 120

Query: 132 DPMPFNLETFGIHTNLGPA--MAPTAQTQAAANATHP-SNAFLLPPPILPPIIAFLFSIF 190
           DP+PFNLETFGIHT+L P+  +AP A   AA  A+H  +NA L+P  +    IA  FS  
Sbjct: 121 DPLPFNLETFGIHTSLDPSQNVAPAAAPTAATKASHANANALLVPTGL--AAIAMFFSTL 179

[Close]

BLAST of Bhi01G002274 vs. NCBI nr
Match: XP_022931314.1 (stellacyanin-like [Cucurbita moschata])

HSP 1 Score: 278.1 bits (710), Expect = 5.6e-71
Identity = 135/192 (70.31%), Postives = 157/192 (81.77%), Query Frame = 0


Query: 1   MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY 60
           MD  +MI   +MMMMGS VNAFTHIVG SHGWRVP+N+TF+DEW KPRTFGVGD+LVFPY
Sbjct: 1   MDNFMMI-MTIMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPY 60

Query: 61  RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI 120
           RPGANN++AVKKADY+ CGEE VI+MY+LGPT+LNLTE GDYYYFDGIGKHCEAGQKLH+
Sbjct: 61  RPGANNIVAVKKADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHV 120

Query: 121 QVGSKEGTSGSDPMPFNLETFGIHTNLGPA--MAPTAQTQAAANATHP-SNAFLLPPPIL 180
           QVG KEGTSG+DP+PFNLETFGIHT+L P   +AP A   AA  A+H  +NA L+P  + 
Sbjct: 121 QVGLKEGTSGADPLPFNLETFGIHTSLDPTQNVAPAAAPTAATKASHANANALLVPAGL- 180

Query: 181 PPIIAFLFSIFL 190
              +A  FS  L
Sbjct: 181 -AAVAMFFSTLL 189

[Close]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL
Match: A0A0A0KUU5 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.8e-78
Identity = 149/204 (73.04%), Postives = 167/204 (81.86%), Query Frame = 0


Query: 1   MDKTVMICC----LVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRL 60
           MDKT+M  C    ++MM+MGSCV+AFTHIVGGSHGWRVPEN +FFD+W KPRTFGVGDRL
Sbjct: 1   MDKTIMNYCYWLVMMMMIMGSCVDAFTHIVGGSHGWRVPENDSFFDQWAKPRTFGVGDRL 60

Query: 61  VFPYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQ 120
           VFPYR GANNL+ VKKADYDTCGEE VI+MYFLGPTV+NLT+ GDYYYFDGIGKHCEAGQ
Sbjct: 61  VFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQ 120

Query: 121 KLHIQVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQA---------AANATHPS 180
           KLHIQVG+KEG+SGSDP+PFNLETFGIHTNLGPA++P  Q  A         +    HPS
Sbjct: 121 KLHIQVGTKEGSSGSDPLPFNLETFGIHTNLGPALSPQGQMDAESVSQAQSPSGTPAHPS 180

Query: 181 NAFLL--PPPILPPIIAFLFSIFL 190
           NAFLL   P +L  II  LFSIFL
Sbjct: 181 NAFLLLPTPMLLALIIPTLFSIFL 204

[Close]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL
Match: A0A6J1J846 (stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.7e-74
Identity = 141/198 (71.21%), Postives = 162/198 (81.82%), Query Frame = 0


Query: 1   MDKTVMICCL--VMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVF 60
           MDK +MI  +  +MMMMGS VNAFTHIVG SHGWRVP+N+TF+DEW KPRTFGVGD+LVF
Sbjct: 1   MDKFMMIMTITTMMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVF 60

Query: 61  PYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKL 120
           PYRPGANN++AVKK DY+ CGEE VI+MY+LGPT+LNLTE GDYYYFDGIGKHCEAGQKL
Sbjct: 61  PYRPGANNIVAVKKTDYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKL 120

Query: 121 HIQVGSKEGTSGSDPMPFNLETFGIHTNL------GPAMAPTAQTQAA-ANATHPSNAFL 180
           H+QVGSKEGTSG+DP+PFNLETFGIHTNL      GPA APTA T+ + AN    +NA L
Sbjct: 121 HVQVGSKEGTSGADPLPFNLETFGIHTNLDPTQNVGPAAAPTAATKGSNANVNANANALL 180

Query: 181 LPPPILPPIIAFLFSIFL 190
           +P  +    IA  FS FL
Sbjct: 181 VPAGL--AAIAMFFSTFL 196

[Close]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL
Match: A0A6J1ETX6 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.7e-71
Identity = 135/192 (70.31%), Postives = 157/192 (81.77%), Query Frame = 0


Query: 1   MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY 60
           MD  +MI   +MMMMGS VNAFTHIVG SHGWRVP+N+TF+DEW KPRTFGVGD+LVFPY
Sbjct: 1   MDNFMMI-MTIMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPY 60

Query: 61  RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI 120
           RPGANN++AVKKADY+ CGEE VI+MY+LGPT+LNLTE GDYYYFDGIGKHCEAGQKLH+
Sbjct: 61  RPGANNIVAVKKADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHV 120

Query: 121 QVGSKEGTSGSDPMPFNLETFGIHTNLGPA--MAPTAQTQAAANATHP-SNAFLLPPPIL 180
           QVG KEGTSG+DP+PFNLETFGIHT+L P   +AP A   AA  A+H  +NA L+P  + 
Sbjct: 121 QVGLKEGTSGADPLPFNLETFGIHTSLDPTQNVAPAAAPTAATKASHANANALLVPAGL- 180

Query: 181 PPIIAFLFSIFL 190
              +A  FS  L
Sbjct: 181 -AAVAMFFSTLL 189

[Close]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL
Match: A0A1S4DYV8 (stellacyanin-like OS=Cucumis melo OX=3656 GN=LOC103493116 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.2e-63
Identity = 127/184 (69.02%), Postives = 146/184 (79.35%), Query Frame = 0


Query: 15  MGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKAD 74
           MGSCV+AFTHIVGGSHGWRVPEN++F           +   +VFPYR GANNL+AVKKAD
Sbjct: 1   MGSCVDAFTHIVGGSHGWRVPENVSFLINGLNQEPLVL--VIVFPYRAGANNLVAVKKAD 60

Query: 75  YDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTSGSDPM 134
           YDTCGEE VI+MYFLGPTV+NLT+ GDYYYFDGIGKHCEAGQKLHIQVG+KEG+SGSDP+
Sbjct: 61  YDTCGEEEVIYMYFLGPTVVNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPL 120

Query: 135 PFNLETFGIHTNLGPAMAPTAQTQAAA-------NATHPSNAFLL--PPPILPPIIAFLF 190
           PFNLETFGIHT+LGPA++P  Q ++ +        AT PSNAFLL   P +L  II  LF
Sbjct: 121 PFNLETFGIHTSLGPALSPQPQAESESKPQSPPGTATPPSNAFLLLPTPMLLALIIPTLF 180

[Close]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL
Match: A0A6J1J638 (umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.7e-63
Identity = 130/196 (66.33%), Postives = 151/196 (77.04%), Query Frame = 0


Query: 6   MICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPYRPGAN 65
           ++ CLV M     ++AFTHIVGG+HGWRVP+N+TFFDEW KPRTFGVGD+LVFPYRPGAN
Sbjct: 12  ILSCLVFM---GSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLVFPYRPGAN 71

Query: 66  NLLAVKKADYDTC--GEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHIQVG 125
           NLLAVKKADY+TC  GEE VI+MY+LGPT+LN+T PGDYYY+DGIGKHCEAGQKLH+QVG
Sbjct: 72  NLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEAGQKLHVQVG 131

Query: 126 SKEGTSGSDPMPFNLETFGIHTNLG-----PAMAPTAQTQAAANATHPSNAF-----LLP 185
           SKEG+SGSDP+PFNL+TFGI T  G     PA AP   T AAA    P+ A      L  
Sbjct: 132 SKEGSSGSDPLPFNLQTFGIPTAFGTTVEPPAAAPVV-TPAAAPVVTPAAAMAHKFGLAS 191

Query: 186 PPILPPIIAFLFSIFL 190
             +L  I+  L SIFL
Sbjct: 192 SAML--IMPVLLSIFL 201

The following BLAST results are available for this feature:

BLAST of Bhi01G002274 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18590.1	7.2e-16	32.30	early nodulin-like protein 5	[more]
AT3G17675.1	1.8e-14	38.38	Cupredoxin superfamily protein	[more]
AT5G20230.1	2.3e-14	30.14	blue-copper-binding protein	[more]
AT2G32300.1	3.0e-14	26.46	uclacyanin 1	[more]
AT5G26330.1	2.6e-13	29.38	Cupredoxin superfamily protein	[more]

BLAST of Bhi01G002274 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P00302	1.7e-14	37.62	Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1	[more]
P42849	1.9e-13	35.24	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
Q07488	3.3e-13	30.14	Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2	[more]
O82081	4.3e-13	26.46	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
Q41001	2.1e-12	27.47	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]

BLAST of Bhi01G002274 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878156.1	2.9e-107	100.00	stellacyanin-like [Benincasa hispida]	[more]
XP_004149757.2	3.7e-78	73.04	blue copper protein [Cucumis sativus] >KGN53385.1 hypothetical protein Csa_01475...	[more]
XP_022985421.1	1.6e-73	71.21	stellacyanin-like [Cucurbita maxima]	[more]
XP_023552874.1	3.3e-71	72.93	stellacyanin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022931314.1	5.6e-71	70.31	stellacyanin-like [Cucurbita moschata]	[more]

BLAST of Bhi01G002274 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUU5	1.8e-78	73.04	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370...	[more]
A0A6J1J846	7.7e-74	71.21	stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1	[more]
A0A6J1ETX6	2.7e-71	70.31	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1	[more]
A0A1S4DYV8	1.2e-63	69.02	stellacyanin-like OS=Cucumis melo OX=3656 GN=LOC103493116 PE=4 SV=1	[more]
A0A6J1J638	2.7e-63	66.33	umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 32..116 e-value: 6.3E-17 score: 61.4
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 22..124 score: 30.022507
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 22..133 e-value: 6.7E-27 score: 95.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 21..122
None	No IPR available	PANTHER	PTHR33021:SF264	OS05G0570900 PROTEIN	coord: 7..150
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..18 score: 5.0
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 7..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M002274	Bhi01M002274	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity