Bhi01G002192 (gene) Wax gourd (B227) v1

Overview
NameBhi01G002192
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCytochrome P450
Locationchr1: 69650565 .. 69653759 (-)
RNA-Seq ExpressionBhi01G002192
SyntenyBhi01G002192
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCTTTTTTTTTTTGTCCATGAAGTTCTCAATCAAACTCATGGACCATACAATATCGTCTCAATCATGAAAGACATGTAGATTAACAACCTCCAGACAGGAAACTTAATCCCGTAATAAATTCTCTCTCGCTCTGGTTTTGAGTAACAATGGAAACTCCTTACACTTCTTGTGTGGTCGCATGTTTCTTCCTCCTCTTCTTCCTCTTTCGTTGTCGTCATAAACTCAATCAACCACCAGGACCAAAGCCATGGCCTTTAATTGGAAATATCCATTTGATTGGCTCTTTACACACCAATCCATTCATCAACTCTCCAAAAAATATGGACCCATCATGCAGCTTCGTTTCGGCTCCTTCCCTGTCGTCGTTGGATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTATCGCCCCAAAAATGCCATCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAGTACGGCCCATATTGGCGTCAAGCAAGGAAGATGTTTATGATGGAACTTTTCAGTCCAAAAAGACTCGACTCTCAAGAGTACATAAGAAAGCAAGAAATGAATGCTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGGATCAAGAATAGAGCTCAAATGCTACCTGTTCAACATGACTTTGAATGTAATAAGTCAGATGGCCTTAGGGAAGAAGTACTTATCGCTGGATCAGTCCAAAGACTCCATTGTTAGTCCAAATGAGTTCAAAGACATGATGGATGAACTCTTCTTGTTAAGTGGTGTACTCAACATAGGAGATTGGATACCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGGATGAAGGCATTGAACAAGAAGTTGGATGGATTTCTTGAGCATATACTAGATGAACATAATGAGAGGAGGAAGAGAATTGAGGATTATGTGGCTAAAGATATGGTGGATGTTTTGCTGCAATTGGTTGATGATCCTAATCTTGAAGTCAAACTTGAAAGGCATGGAGTCAAGGCACTTATTCAGGTATACACTCTCCTATCCGTGTCTCTCAACTTTAAAAATGTTTAATACGTTGTTCAATTTTAATTTTGATTTATTTGTTTGATTAGATCTCTTAAATTTTAAAACTTTCTAATACATTCTTTAGCTTTCAATTTTGTGAAAATATATTTAAAAAATTAAAAATCTTTAGTATCAAGACAAACAAAGACAGAACACTAAAGGGATTTTGATTCAACTAGTAAATAAGTAATTGGTATCCATCTATGTCTTCGAGCATGAAACGCCAGTTCGTGGGCCACACTATGTTGCACTTACAAATGTAATAAAATTTAAAATTCAATTAACTTAGACCAATATGGTAATCATTTTGTTTTATGTTTTTAGTTTTTAAAAAATAAGTATACAAATACTTCTTCCACCTCTAAATTTGTTGTTATGTCATCTATTTTTTACTCATGTCTTTAAAAACCAAACCAACTTTTGAAAATTATAGTTTTTAAAAATGTTGGTAATTAAAGAATACAAAATAAACATAAAGAAAGATCGACACACAAATATACGTGGTTCGCTAACAGTGTGTTACATCTATAAGGTAGAAGGAGAACGGTATTATTAAAGAGAATATTTTCTGAATACAAAACGACACCACTAAGGTTAGAGAGTTTATAGAGCGAACTTTCTCTAGACCCTAGGGTCATCGTAAATAGTTATAAATAATTAAATATGCTAAATACGAATTTTGAACCACAAGACCCCCATACTTGGTTGAGAAGTACCATCTAACAACTTCAAGGCTTTCCAACAAAAAAAAAACATTTTTTAAAAAAAAAAAATTGGTTAAGAATTTAACTCTATTTAAGAAAAATGTAAACTATGGTAAAAAGATGAGAGGAAATGGGCTTTTTCAAAAACTAAAAATAAAAGACGGAATGGTTACTAAACGAGTCACTAACTTCCAATTAGATTGAATTGTGACACACATGACTCTTAACTTTCAATTTTGTAACTAGTATGTCTCTAATTTTTTTTTTAAAAAAAAACTATCAAATAGTTAGGCTAGAGATAAAAATGAAATTAATCTAGAGACAAACTTATTTGATACGAAACTAAAAGTTAAAAGAACGTATATATTATACGATAATTTTTAAGGTTTAGTAACCTGTTAAAAACAAATTGAAAGTTGAAAATTTATTAAAAACTTTTTATAGTTCAGAACCAATTAATAAAGAAAAATCTAAAAGTTTAGGAACTAAACTTGGCGGAATATTATCTATTTTAGGACATTATATCAGGTGGAACAGATACCTCAGTTTTGACAGTAGAATGGGCAATGTCCGAGCTCTTGAAAAAACCAGAGATTTTCGATAAAGCAATAGAAGAGCTTGACAGAGTAATTGGAAAAGAAAGATGGGTAGAAGAAAAAGACATGGAAAAGTTGGCATACATTGAAGCAATTGCAAAAGAGACAATGAGATTGCACCCTGCAGCGCCACTGTTGGTTCCAAGATTGTGTAGAGAAGCTTGTGAAGTTGGAGGTTACAATATAAACAAAGGCACTAGAGTAGTTGTGAATGTATGGGCAATTGGAAGAGACCCAACAGTTTGGGAAAATCCAAATGAGTTTCAACCACAAAGATTTGTTGAGAAAAACATGGATATAAAAGGATTGCGCAACTTTGAATTTTTGCCATTTGGTTCTGGAAGGAGGATGTGCCCTGGTTACAATCTTGGTTTTAAAGTTATATTGTCAACTTTGGCCAATCTCTTGCATGGCTTTTCTTGGAGATTGCCTGACAATATGAACAATGAGGATCTTAATATGGATGAAGTTTTTGGACTCACAGTTCCTAGGAAATTCCCTCTCATTGCTCTTGCTAAACCAAGACTCCCTCTTCATAATTATAAATTGGAATGAGAGACCAAAATCCTTTATATATCTTGGGGCTACTTTTGCTTCTTTTCCTATGATGTTGTTTTTATGTATTATTGTGTATCCTATTGTTTTGTGATAAATGTGTGGTTTTCAAGTTTCAATTTAGGTTGGTTTATGTTGTCACATCTTCACCAAACAATAAATAATATGGTATTGTCTATTTTAAGCAACTTTCTATTACTTCTACTTTTTTGTTTAATTGAAAGAAAAATCCGTGTTATTAACTTTTCACTTAAGG

mRNA sequence

CGGCTTTTTTTTTTTGTCCATGAAGTTCTCAATCAAACTCATGGACCATACAATATCGTCTCAATCATGAAAGACATGTAGATTAACAACCTCCAGACAGGAAACTTAATCCCGTAATAAATTCTCTCTCGCTCTGGTTTTGAGTAACAATGGAAACTCCTTACACTTCTTGTGTGGTCGCATGTTTCTTCCTCCTCTTCTTCCTCTTTCGTTGTCGTCATAAACTCAATCAACCACCAGGACCAAAGCCATGGCCTTTAATTGGAAATATCCATTTGATTGGCTCTTTACACACCAATCCATTCATCAACTCTCCAAAAAATATGGACCCATCATGCAGCTTCGTTTCGGCTCCTTCCCTGTCGTCGTTGGATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTATCGCCCCAAAAATGCCATCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAGTACGGCCCATATTGGCGTCAAGCAAGGAAGATGTTTATGATGGAACTTTTCAGTCCAAAAAGACTCGACTCTCAAGAGTACATAAGAAAGCAAGAAATGAATGCTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGGATCAAGAATAGAGCTCAAATGCTACCTGTTCAACATGACTTTGAATGTAATAAGTCAGATGGCCTTAGGGAAGAAGTACTTATCGCTGGATCAGTCCAAAGACTCCATTGTTAGTCCAAATGAGTTCAAAGACATGATGGATGAACTCTTCTTGTTAAGTGGTGTACTCAACATAGGAGATTGGATACCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGGATGAAGGCATTGAACAAGAAGTTGGATGGATTTCTTGAGCATATACTAGATGAACATAATGAGAGGAGGAAGAGAATTGAGGATTATGTGGCTAAAGATATGGTGGATGTTTTGCTGCAATTGGTTGATGATCCTAATCTTGAAGTCAAACTTGAAAGGCATGGAGTCAAGGCACTTATTCAGGACATTATATCAGGTGGAACAGATACCTCAGTTTTGACAGTAGAATGGGCAATGTCCGAGCTCTTGAAAAAACCAGAGATTTTCGATAAAGCAATAGAAGAGCTTGACAGAGTAATTGGAAAAGAAAGATGGGTAGAAGAAAAAGACATGGAAAAGTTGGCATACATTGAAGCAATTGCAAAAGAGACAATGAGATTGCACCCTGCAGCGCCACTGTTGGTTCCAAGATTGTGTAGAGAAGCTTGTGAAGTTGGAGGTTACAATATAAACAAAGGCACTAGAGTAGTTGTGAATGTATGGGCAATTGGAAGAGACCCAACAGTTTGGGAAAATCCAAATGAGTTTCAACCACAAAGATTTGTTGAGAAAAACATGGATATAAAAGGATTGCGCAACTTTGAATTTTTGCCATTTGGTTCTGGAAGGAGGATGTGCCCTGGTTACAATCTTGGTTTTAAAGTTATATTGTCAACTTTGGCCAATCTCTTGCATGGCTTTTCTTGGAGATTGCCTGACAATATGAACAATGAGGATCTTAATATGGATGAAGTTTTTGGACTCACAGTTCCTAGGAAATTCCCTCTCATTGCTCTTGCTAAACCAAGACTCCCTCTTCATAATTATAAATTGGAATGAGAGACCAAAATCCTTTATATATCTTGGGGCTACTTTTGCTTCTTTTCCTATGATGTTGTTTTTATGTATTATTGTGTATCCTATTGTTTTGTGATAAATGTGTGGTTTTCAAGTTTCAATTTAGGTTGGTTTATGTTGTCACATCTTCACCAAACAATAAATAATATGGTATTGTCTATTTTAAGCAACTTTCTATTACTTCTACTTTTTTGTTTAATTGAAAGAAAAATCCGTGTTATTAACTTTTCACTTAAGG

Coding sequence (CDS)

ATGGCCTTTAATTGGAAATATCCATTTGATTGGCTCTTTACACACCAATCCATTCATCAACTCTCCAAAAAATATGGACCCATCATGCAGCTTCGTTTCGGCTCCTTCCCTGTCGTCGTTGGATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTATCGCCCCAAAAATGCCATCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAGTACGGCCCATATTGGCGTCAAGCAAGGAAGATGTTTATGATGGAACTTTTCAGTCCAAAAAGACTCGACTCTCAAGAGTACATAAGAAAGCAAGAAATGAATGCTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGGATCAAGAATAGAGCTCAAATGCTACCTGTTCAACATGACTTTGAATGTAATAAGTCAGATGGCCTTAGGGAAGAAGTACTTATCGCTGGATCAGTCCAAAGACTCCATTGTTAGTCCAAATGAGTTCAAAGACATGATGGATGAACTCTTCTTGTTAAGTGGTGTACTCAACATAGGAGATTGGATACCATGGATTGATTTCTTGGATTTGCAAGGGTATGTGAAGAGGATGAAGGCATTGAACAAGAAGTTGGATGGATTTCTTGAGCATATACTAGATGAACATAATGAGAGGAGGAAGAGAATTGAGGATTATGTGGCTAAAGATATGGTGGATGTTTTGCTGCAATTGGTTGATGATCCTAATCTTGAAGTCAAACTTGAAAGGCATGGAGTCAAGGCACTTATTCAGGACATTATATCAGGTGGAACAGATACCTCAGTTTTGACAGTAGAATGGGCAATGTCCGAGCTCTTGAAAAAACCAGAGATTTTCGATAAAGCAATAGAAGAGCTTGACAGAGTAATTGGAAAAGAAAGATGGGTAGAAGAAAAAGACATGGAAAAGTTGGCATACATTGAAGCAATTGCAAAAGAGACAATGAGATTGCACCCTGCAGCGCCACTGTTGGTTCCAAGATTGTGTAGAGAAGCTTGTGAAGTTGGAGGTTACAATATAAACAAAGGCACTAGAGTAGTTGTGAATGTATGGGCAATTGGAAGAGACCCAACAGTTTGGGAAAATCCAAATGAGTTTCAACCACAAAGATTTGTTGAGAAAAACATGGATATAAAAGGATTGCGCAACTTTGAATTTTTGCCATTTGGTTCTGGAAGGAGGATGTGCCCTGGTTACAATCTTGGTTTTAAAGTTATATTGTCAACTTTGGCCAATCTCTTGCATGGCTTTTCTTGGAGATTGCCTGACAATATGAACAATGAGGATCTTAATATGGATGAAGTTTTTGGACTCACAGTTCCTAGGAAATTCCCTCTCATTGCTCTTGCTAAACCAAGACTCCCTCTTCATAATTATAAATTGGAATGA

Protein sequence

MAFNWKYPFDWLFTHQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNYSNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELKCYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWIDFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVWENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKLE
Homology
BLAST of Bhi01G002192 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 6.1e-96
Identity = 183/468 (39.10%), Postives = 282/468 (60.26%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H+++  +   YGPI+ LR G   VVV +S  +A+ FLK HD  F  RP N+  K++ YNY
Sbjct: 54  HRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNY 113

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            ++ ++ YG  WR  RK+  + LFS K L+  +++R++E+    +E+ +    G++    
Sbjct: 114 QDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRV---GTKPVNL 173

Query: 135 CYLFNM-TLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWI 194
             L NM  +N + +  +G++    D    +    +EF+ M+ E+  L+GV NIGD++P +
Sbjct: 174 GQLVNMCVVNALGREMIGRRLFGADADHKA----DEFRSMVTEMMALAGVFNIGDFVPSL 233

Query: 195 DFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQL--VDDPNL 254
           D+LDLQG   +MK L+K+ D FL  IL EH       +D    DM+  L+ L   D    
Sbjct: 234 DWLDLQGVAGKMKRLHKRFDAFLSSILKEHEMNG---QDQKHTDMLSTLISLKGTDLDGD 293

Query: 255 EVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVE 314
              L    +KAL+ ++ + GTDTS  TV+WA++EL++ P+I  KA EELD V+G++R V 
Sbjct: 294 GGSLTDTEIKALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVN 353

Query: 315 EKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDP 374
           E D+ +L Y++A+ KE  RLHP  PL +P +  E+CE+ GY+I KG+ ++ N+WAI RDP
Sbjct: 354 ESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDP 413

Query: 375 TVWENPNEFQPQRFV----EKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANL 434
             W +P  F+P+RF+    +  +D+KG  +FE +PFG+GRR+C G +LG + I    A L
Sbjct: 414 DQWSDPLAFKPERFLPGGEKSGVDVKG-SDFELIPFGAGRRICAGLSLGLRTIQFLTATL 473

Query: 435 LHGFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           + GF W L   +  E LNM+E +GLT+ R  PL+   KPRL  + Y L
Sbjct: 474 VQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVYGL 510

BLAST of Bhi01G002192 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 329.3 bits (843), Expect = 5.0e-90
Identity = 185/463 (39.96%), Postives = 273/463 (58.96%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H     L++ +GPI +L  GS   +V +S  +A+  LK  D+ F  R     G+  TY  
Sbjct: 63  HTYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGG 122

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            +I W+ YG  WRQ RK+ +++L S K LDS   +R++E+    + +Y+   + S +++ 
Sbjct: 123 IDIVWTPYGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVG 182

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
             LF   +N+   M  G    +  +  +S+    EFK ++ E+  L    N+ D+ PW+ 
Sbjct: 183 DQLFLTMMNLTMNMLWGGSVKA--EEMESV--GTEFKGVISEITRLLSEPNVSDFFPWLA 242

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVD-DPNLEV 254
             DLQG VKRM    ++LD  L+  +++    R R +D V KD +  L++L D + + EV
Sbjct: 243 RFDLQGLVKRMGVCARELDAVLDRAIEQMKPLRGRDDDEV-KDFLQYLMKLKDQEGDSEV 302

Query: 255 KLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEK 314
            +  + VKAL+ D++ GGTDTS  T+E+AM+EL+  PE+  +A EELD V+GK+  VEE 
Sbjct: 303 PITINHVKALLTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEES 362

Query: 315 DMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTV 374
            + +L YI AI KET+RLHP  PLLVP    E   VGGY I K T++ VNVW+I RDP V
Sbjct: 363 HITRLPYILAIMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNV 422

Query: 375 WENPNEFQPQRFVEKN-MDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFS 434
           WENP EF+P+RF++ N  D  G  N+ + PFGSGRR+C G  L  +++L TLA LLH F 
Sbjct: 423 WENPTEFRPERFLDNNSCDFTG-ANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFD 482

Query: 435 WRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           W++P+      L++ E FG+ +  K PL+AL  PR    N  L
Sbjct: 483 WKIPE---GHVLDLKEKFGIVLKLKIPLVALPIPRFSDSNLYL 516

BLAST of Bhi01G002192 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 327.8 bits (839), Expect = 1.5e-89
Identity = 171/455 (37.58%), Postives = 271/455 (59.56%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H     L+ KYGP+M++  GS   +V SS +MA+  LKTHD+TF       +GK  TY  
Sbjct: 75  HTQFQALTLKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGG 134

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            +I WS YG +WR+ RK+ +M++F+   L++    R++E    +  + + + +GS + L 
Sbjct: 135 EDILWSPYGTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREGSPVNLG 194

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
             +F    NV+++M  G    +  +  +     NE K ++ ++  + G+ N  D+ P   
Sbjct: 195 EQIFLSIFNVVTRMMWG----ATVEGDERTSLGNELKTLISDISDIEGIQNYSDFFPLFS 254

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNER-RKRIEDYVAKDMVDVLLQLVDDPNLEV 254
             D QG VK+MK   KKLD   + +++ H +   K+ E+   +D +  LL++ DD   + 
Sbjct: 255 RFDFQGLVKQMKGHVKKLDLLFDRVMESHVKMVGKKSEE--EEDFLQYLLRVKDDDE-KA 314

Query: 255 KLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEK 314
            L    VK+L+ D++ GG DTSV   E+AM+E++ +PE+ +K   ELD+V+GK+  VEE 
Sbjct: 315 PLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEES 374

Query: 315 DMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTV 374
            + KL Y++A+ KET+RLHP  PLLVP    E   V GY + K +++ +NVWAI RDP  
Sbjct: 375 HLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKN 434

Query: 375 WENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSW 434
           W+ PNEF+P+RF+E ++D  G  +F++LPFGSGRR+C   N+  +++L  +A+LLH F W
Sbjct: 435 WDEPNEFKPERFLENSLDFNG-GDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDW 494

Query: 435 RLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           + P     E   ++E FGL +  K PL+A+  PRL
Sbjct: 495 KAPQGQKFE---VEEKFGLVLKLKSPLVAIPVPRL 518

BLAST of Bhi01G002192 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 326.6 bits (836), Expect = 3.2e-89
Identity = 182/463 (39.31%), Postives = 275/463 (59.40%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQS+  LSKKYGP+M L+FGS P VV SS E AK  LK HDL    RP  A  + ++YNY
Sbjct: 52  HQSLWSLSKKYGPVMLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNY 111

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            +I +S +  YW++ R+M + ELFSPK++   + IR++E+   +    +++ Q + + L 
Sbjct: 112 LDIVFSPFNDYWKELRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLS 171

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIP--- 194
             L ++T+ VI + A G  +      + ++++ + F  ++ + FL  G  +  D+ P   
Sbjct: 172 EKLASLTVGVICKAAFGVSF------QGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVG 231

Query: 195 W-IDFL-DLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDP 254
           W ID+L  LQG  +R     + LD F E + D H +  K       +D VD+LL+L  + 
Sbjct: 232 WIIDWLTGLQGQRERSV---RGLDAFYEQMFDLHKQGNKE----GVEDFVDLLLKLEKEE 291

Query: 255 NL--EVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKE 314
            +    KL R+ +KA++ +++ GG  TS +T+ WAM+EL++ P +  K   E+   IG +
Sbjct: 292 TVLGYGKLTRNHIKAVLMNVLLGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGK 351

Query: 315 RWVEEKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAI 374
             +   D+++L Y++ +  ET RLHP APLLVPR      E+ GY I   TR+ VNVW I
Sbjct: 352 SMICLDDIDQLHYLKMVINETWRLHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGI 411

Query: 375 GRDPTVWENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANL 434
           GRDP  W++P EF P+RFV  N+D KG +NFE LPFGSGRRMCP   +G  ++   LANL
Sbjct: 412 GRDPDTWKDPEEFLPERFVNSNIDAKG-QNFELLPFGSGRRMCPAMYMGTTMVEFGLANL 471

Query: 435 LHGFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPL 471
           L+ F W+LP+ M  ED++M+E  GL   +K  L+ + +  L L
Sbjct: 472 LYHFDWKLPEGMVVEDIDMEESPGLNASKKNELVLVPRKYLNL 500

BLAST of Bhi01G002192 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 325.9 bits (834), Expect = 5.5e-89
Identity = 170/455 (37.36%), Postives = 271/455 (59.56%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H     L+ K+GP+M++  GS   +V SS +MA+  LKTHD+TF       +GK  TY  
Sbjct: 106 HTQFQALTLKHGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGG 165

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            +I WS YG +WR+ RK+ +M++F+   L++    R++E    +  + + + +GS + L 
Sbjct: 166 EDILWSPYGTHWRRLRKLCVMKMFTTPTLEASYSTRREETRQTIVHMSEMAREGSPVNLG 225

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
             +F    NV+++M  G    +  +  +     NE K ++ ++  + G+ N  D+ P   
Sbjct: 226 EQIFLSIFNVVTRMMWG----ATVEGDERTSLGNELKTLISDISDIEGIQNYSDFFPLFS 285

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNER-RKRIEDYVAKDMVDVLLQLVDDPNLEV 254
             D QG VK+MK   KKLD   + +++ H +   K+ E+   +D +  LL++ DD   + 
Sbjct: 286 RFDFQGLVKQMKGHVKKLDLLFDRVMESHVKMVGKKSEE--EEDFLQYLLRVKDDDE-KA 345

Query: 255 KLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEK 314
            L    VK+L+ D++ GG DTSV   E+AM+E++ +PE+ +K   ELD+V+GK+  VEE 
Sbjct: 346 PLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEES 405

Query: 315 DMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTV 374
            + KL Y++A+ KET+RLHP  PLLVP    E   V GY + K +++ +NVWAI RDP  
Sbjct: 406 HLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKN 465

Query: 375 WENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSW 434
           W+ PNEF+P+RF+E ++D  G  +F++LPFGSGRR+C   N+  +++L  +A+LLH F W
Sbjct: 466 WDEPNEFKPERFLENSLDFNG-GDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDW 525

Query: 435 RLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           + P     E   ++E FGL +  K PL+A+  PRL
Sbjct: 526 KAPQGQKFE---VEEKFGLVLKLKSPLVAIPVPRL 549

BLAST of Bhi01G002192 vs. ExPASy Swiss-Prot
Match: A0A1D6F9Y9 (Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.7e-119
Identity = 223/479 (46.56%), Postives = 320/479 (66.81%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H+++  L+ ++GP+M LR GS+  VV SS + A+L LK HDL F  RP+ A G+  +Y Y
Sbjct: 55  HRALAALAARHGPLMHLRLGSYHAVVASSADTARLVLKVHDLAFADRPRTAAGEVASYGY 114

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
             I  + YG YWR ARK+   ELFS +R+DS E +R QEM A  + +++ + +G+ + ++
Sbjct: 115 LGIVHTPYGAYWRMARKLCATELFSARRVDSFERVRAQEMRALARGLFECAGRGA-VAVR 174

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSG-VLNIGDWIPWI 194
            ++   TL  I +MA+G+K+     S +       F+  +DE F ++G V N+G+W+PW+
Sbjct: 175 EHVAGATLRNILRMAVGEKWSGCYGSPEG----EAFRRTLDEAFAVTGAVSNVGEWVPWL 234

Query: 195 DFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIE-------DYVA-KDMVDVLLQL 254
            +LDLQG V+RMK L  + D F E ILD+H+ ++ R E       D  A  D+VDVLL+L
Sbjct: 235 GWLDLQGCVRRMKRLRAQYDRFFEQILDDHDHKKTRRERGRPGAGDSAADDDLVDVLLRL 294

Query: 255 V--DDPNLEV----KLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEE 314
           V  D+   E     +L R GVKA +QDI++GGT++S +T+EWAMSELL++P+    A  E
Sbjct: 295 VEEDEDRPETTEASRLTRDGVKAFVQDIVAGGTESSAVTIEWAMSELLRRPDALRAATAE 354

Query: 315 LDRVIGKERWVEEKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTR 374
           LDRVIG  RWV E+D+  L YI+A+ KETMRLHP  PLLVP   RE   V GY++  G R
Sbjct: 355 LDRVIGHGRWVTERDLPDLPYIDAVVKETMRLHPVGPLLVPHHAREHTVVAGYDVPAGAR 414

Query: 375 VVVNVWAIGRDPTVW-ENPNEFQPQRFVE----KNMDIKGLRNFEFLPFGSGRRMCPGYN 434
           V+VNVWAI RDP  W + P+ F+P+RF+      ++D++G  +FE LPFG+GRRMCP + 
Sbjct: 415 VLVNVWAIARDPASWPDAPDAFRPERFLNGSSGASVDVRG-AHFELLPFGAGRRMCPAHG 474

Query: 435 LGFKVILSTLANLLHGFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNY 474
           L  K++ + +ANL+HGF+WRLPD M  ED++M+E+FGL+  RK PL+A+A+PRLP H Y
Sbjct: 475 LAMKLVTAGVANLVHGFAWRLPDGMAPEDVSMEELFGLSTRRKVPLVAVAEPRLPAHLY 527

BLAST of Bhi01G002192 vs. ExPASy Swiss-Prot
Match: A0A1D6HSP4 (Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.0e-116
Identity = 212/472 (44.92%), Postives = 312/472 (66.10%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H+++  L+ ++GP+M LR GS+  VV SS + A+L L+THD     RP  A G+  +Y Y
Sbjct: 61  HRALAALAARHGPLMHLRLGSYHTVVASSADAARLVLRTHDSALADRPDTAAGEITSYGY 120

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
             I  +  G YWR AR++   ELFS +R++S + +R QEM A  + ++  +     + ++
Sbjct: 121 LGIVHTPRGAYWRMARRLCATELFSARRVESFQDVRAQEMRALARGLFGCAAGRRAVAVR 180

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSG-VLNIGDWIPWI 194
            ++   T+  I +MA+G+K+     S +       F+  +DE F  +G V N+G+W+PW+
Sbjct: 181 EHVAGATMRNILRMAVGEKWSGCYGSPEG----EAFRRSLDEAFAATGAVSNVGEWVPWL 240

Query: 195 DFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIE----DYVAKDMVDVLLQLVD-- 254
            +LD+QG+ ++MK L+   D F E IL +H ERR+  +    ++VA D+VDVLLQL +  
Sbjct: 241 GWLDVQGFKRKMKRLHDLHDHFYEKILVDHEERRRLAQASGGEFVATDLVDVLLQLSEES 300

Query: 255 ---DPNLEVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVI 314
              +   E +L R GVKALIQDII+GGT++S +T+EWAM+ELL+ PE   KA +ELDRV+
Sbjct: 301 TKLESESEARLPRDGVKALIQDIIAGGTESSAVTIEWAMAELLRHPEAMAKATDELDRVV 360

Query: 315 GKERWVEEKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNV 374
           G  RWV E+D+ +L YI+A+ KET+RLHP  PLLVP   RE   V GY++  G RV+VN 
Sbjct: 361 GSGRWVAERDLPELHYIDAVVKETLRLHPVGPLLVPHYARERTVVAGYDVPAGARVLVNA 420

Query: 375 WAIGRDPTVW-ENPNEFQPQRFV--EKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVIL 434
           WAI RDP  W + P+ FQP+RF+     +D++G  +FE LPFGSGRR+CP Y+L  K++ 
Sbjct: 421 WAIARDPASWPDAPDAFQPERFLGAAAAVDVRG-AHFELLPFGSGRRICPAYDLAMKLVA 480

Query: 435 STLANLLHGFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNY 474
           + +ANL+HGF+WRLPD +  ED++M+E  GL+  RK PL A+A+PRLP+H Y
Sbjct: 481 AGVANLVHGFAWRLPDGVAAEDVSMEEHVGLSTRRKVPLFAVAEPRLPVHLY 527

BLAST of Bhi01G002192 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 1.2e-104
Identity = 195/459 (42.48%), Postives = 291/459 (63.40%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           H+S+  L+ + GP++ L  G  P ++ S+ E+A+  LKTHDL F  RP     + + Y+ 
Sbjct: 52  HRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDC 111

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           +++ +S YG YWRQ RK+ ++EL S KR++S   IR++E+   ++ I ++   G  + L 
Sbjct: 112 TDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLS 171

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIP--- 194
             L  ++   I+++A GKKY   ++ K      N+F D+  EL  L G   +GD+ P   
Sbjct: 172 ELLLLLSSGTITRVAFGKKYEGEEERK------NKFADLATELTTLMGAFFVGDYFPSFA 231

Query: 195 WIDFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKR--IEDYVAKDMVDVLLQLVDDP 254
           W+D   L G   R+K  + +LD F++H++D+H   RK    +    KD+VDVLL L  D 
Sbjct: 232 WVDV--LTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDS 291

Query: 255 NLEVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERW 314
           +L V L R+ +KA+I D+ SGGTDT+ +T+EWAM+EL+K P++ +KA +E+ RV+GK+  
Sbjct: 292 SLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAK 351

Query: 315 VEEKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGR 374
           VEE+D+ +L Y++ I KET+RLHP APLLVPR       + GY+I   TRV +N WAIGR
Sbjct: 352 VEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGR 411

Query: 375 DPTVWENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLH 434
           DP  WEN  EF P+RFV  ++D KG ++F+ +PFG+GRR CPG   G   +  +LANLL+
Sbjct: 412 DPKSWENAEEFLPERFVNNSVDFKG-QDFQLIPFGAGRRGCPGIAFGISSVEISLANLLY 471

Query: 435 GFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
            F+W LP ++  EDL+M E  G+TV  KFPL  +AK  L
Sbjct: 472 WFNWELPGDLTKEDLDMSEAVGITVHMKFPLQLVAKRHL 501

BLAST of Bhi01G002192 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.3e-103
Identity = 190/457 (41.58%), Postives = 290/457 (63.46%), Query Frame = 0

Query: 13  FTHQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTY 72
           F ++ +H+L++KYG IM +R G  P +V SS E A+LFLKTHDL F  RP +   K++++
Sbjct: 47  FPNKDLHRLARKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISF 106

Query: 73  NYSNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEI-YKASCQGSRI 132
              N+ +S+YG YWR  RKM  +EL S  +++S + +R++E++  ++ I   A+ +G  +
Sbjct: 107 GQKNLIFSEYGAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAV 166

Query: 133 ELKCYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIP 192
           +L   + ++++++  +M LGKKY      +D       FK ++ E   L+   N+GD+I 
Sbjct: 167 DLSDKVSSLSVDMSCRMVLGKKY------RDEEFDERGFKSVVREAIQLASAPNLGDYIR 226

Query: 193 WIDFLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNL 252
           +I  LDLQG+ KRMK++NK  D   E I++EH +     +     D VDV++  +     
Sbjct: 227 FIAPLDLQGFTKRMKSVNKAFDNLFEKIIEEHLQPN---DGERTMDFVDVMVGFMGSEES 286

Query: 253 EVKLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVE 312
           E ++ER  +KA++ D++    DTS  T+EWA+SEL++ P+   K  +EL+ V+G ++ VE
Sbjct: 287 EYRIERPHIKAIMLDMLVASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVE 346

Query: 313 EKDMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDP 372
           E D+EKL Y+  + KET RLHP APLL+P    E C V GY+I K +RV++NVWAIGRDP
Sbjct: 347 ESDLEKLDYLNMVVKETFRLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDP 406

Query: 373 TVWENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGF 432
             W +  +F P+RF   ++D++G  +F+ +PFGSGRR CPG  LG  V+   LA L+H F
Sbjct: 407 NAWTDAEKFIPERFEGSSVDVRG-NHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCF 466

Query: 433 SWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
            W LP+NM  E+L+M E FGLTVPR   L+A+   RL
Sbjct: 467 DWELPNNMLPEELDMTEEFGLTVPRAKHLLAIPSYRL 493

BLAST of Bhi01G002192 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.3e-100
Identity = 183/459 (39.87%), Postives = 290/459 (63.18%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQ++  LSK+YGP+ +LR GS  VVV SS  +A  FL+THD+ F  RP N+  ++V YNY
Sbjct: 54  HQTLDALSKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNY 113

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
            ++ ++ YGP WR  RK+  + LFS K LD    +R+ E+   ++ + + +  G  + L 
Sbjct: 114 QDLVFAPYGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLG 173

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
             L     N +++  LG++  + + ++  +   +EFK+M+ EL  L+GV N+GD++P + 
Sbjct: 174 KALNVCATNALARAMLGRRVFADEDAQ--LAEADEFKEMVVELMRLAGVFNVGDFVPGLG 233

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVD-DPNLEV 254
           +LDLQG V +MK L+++ D FL+ +++E+    K      + D++ VL++L + D   E+
Sbjct: 234 WLDLQGVVGKMKRLHRRYDAFLDRVIEENQANAK------SGDLLSVLIRLKEADAEGEI 293

Query: 255 KLERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEK 314
           KL    +KAL+ ++ + GTDTS  TVEW ++EL++ P+I  K   ELD VIG++R V E 
Sbjct: 294 KLNNTDIKALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAES 353

Query: 315 DMEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTV 374
           D+  L Y++A+ KET RLHP+ PL +PR+  E C V GY I K   ++VNVW+IGRD  V
Sbjct: 354 DLPNLPYLQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAV 413

Query: 375 WENPNEFQPQRFV----EKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLH 434
           W +P EF+P RF+     +++D+KG  +FE +PFG+GRR+C G +LG +++    A ++H
Sbjct: 414 WNDPLEFRPSRFLPGGEREHVDVKG-NDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVH 473

Query: 435 GFSWRLPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
            + W LP     + L+M+E +GLT+ R  PL+    PRL
Sbjct: 474 AYDWSLPKGQECQKLDMEEAYGLTLQRAVPLMVQPIPRL 503

BLAST of Bhi01G002192 vs. NCBI nr
Match: XP_038904918.1 (LOW QUALITY PROTEIN: trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 943.3 bits (2437), Expect = 7.9e-271
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY
Sbjct: 49  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 108

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK
Sbjct: 109 SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 168

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
           CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID
Sbjct: 169 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 228

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK
Sbjct: 229 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 288

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD
Sbjct: 289 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 348

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW
Sbjct: 349 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 408

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR
Sbjct: 409 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 468

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKLE 477
           LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKLE
Sbjct: 469 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKLE 510

BLAST of Bhi01G002192 vs. NCBI nr
Match: XP_038877760.1 (trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 715.7 bits (1846), Expect = 2.7e-202
Identity = 345/461 (74.84%), Postives = 403/461 (87.42%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKTHDL FV RPK A GKY TYNY
Sbjct: 56  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNY 115

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS +RLDS EYIRK+EMNA L++IYK+  +G  I+LK
Sbjct: 116 SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLKDIYKS--RGQVIKLK 175

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LG+KY   D+S+++IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 176 DYLSTVSLNVISRMVLGRKY--TDESENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 235

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHNERRK +E+YVAKDMVDVLLQ  DDPNLEVK
Sbjct: 236 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVENYVAKDMVDVLLQQADDPNLEVK 295

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +T+EWA+SELLK+PEIFDKA EELDRVIG+ERWVEEKD
Sbjct: 296 LERHGVKAFTQDLIAGGTESSAVTIEWAISELLKQPEIFDKATEELDRVIGRERWVEEKD 355

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI AIAKETMRLHP AP+LVPR+CRE C++ GY+I KGTRV+VNVW IGRDPTVW
Sbjct: 356 IVNLPYINAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPTVW 415

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           +NP  F P+RF+E ++D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGFSW+
Sbjct: 416 KNPYAFDPERFMENSIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWK 475

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           LP +M  EDLN++E+FGL+ P+KFPL A+A PRLP H Y L
Sbjct: 476 LPGDMEKEDLNLEEIFGLSTPKKFPLDAVADPRLPPHLYSL 511

BLAST of Bhi01G002192 vs. NCBI nr
Match: XP_022152553.1 (cytochrome P450 71A1-like [Momordica charantia])

HSP 1 Score: 711.4 bits (1835), Expect = 5.0e-201
Identity = 345/461 (74.84%), Postives = 404/461 (87.64%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLS+KYG IM LRFGSFPVVVGSSVEMAK+FLKTHDLTFV RPK A GKY TYNY
Sbjct: 54  HQSIHQLSRKYGSIMHLRFGSFPVVVGSSVEMAKIFLKTHDLTFVSRPKTAAGKYTTYNY 113

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS KRLDS EYIR++EM+A L++IY+ SC G  I++K
Sbjct: 114 SDITWSQYGPYWRQARKMCLMELFSAKRLDSYEYIRREEMSALLRDIYR-SC-GRAIQVK 173

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S+++IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 174 DYLSTVSLNVISRMVLGKKY--TDESENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 233

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGY+KRMKAL+KK D FLEH+LDEHNERRK I+DYVAKDMVDVLLQL DDP+LEVK
Sbjct: 234 FLDLQGYIKRMKALSKKFDRFLEHVLDEHNERRKGIKDYVAKDMVDVLLQLADDPDLEVK 293

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF KA EELDRVIGKERWVEEKD
Sbjct: 294 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFIKATEELDRVIGKERWVEEKD 353

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I K TR++VNVW IGRDP VW
Sbjct: 354 ITNLPYIDAIAKETMRLHPVAPMLVPRMSREDCKIAGYDIAKDTRILVNVWTIGRDPEVW 413

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENPNEF P+RF+ K++D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGFSW+
Sbjct: 414 ENPNEFNPERFIGKSIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWK 473

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           L  +M  EDLNM+E+FGL+ P+KFPL+A+A+PRLP H Y +
Sbjct: 474 LGGDMRKEDLNMEEIFGLSTPKKFPLVAVAEPRLPPHLYSM 509

BLAST of Bhi01G002192 vs. NCBI nr
Match: XP_008460308.1 (PREDICTED: cytochrome P450 71A1-like [Cucumis melo])

HSP 1 Score: 711.1 bits (1834), Expect = 6.6e-201
Identity = 350/454 (77.09%), Postives = 399/454 (87.89%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKT DL FV RPK A GKY TYNY
Sbjct: 53  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNY 112

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS +RLDS EYIRK+EMNA L+EIYK SC G  I++K
Sbjct: 113 SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYK-SC-GEVIKIK 172

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 173 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 232

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHNERRK +EDYVAKDMVDVLLQL DDP+LEVK
Sbjct: 233 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVK 292

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF+KA EELDRVIGKERWVEEKD
Sbjct: 293 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKD 352

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I KGTRV VNVW IGRDPTVW
Sbjct: 353 IINLPYIDAIAKETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVW 412

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENP EF+P+RF+ KN+D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGF+W+
Sbjct: 413 ENPLEFKPERFMGKNIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWK 472

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           L  +M NEDLNMDEVFGL+ P+KFPL  +A+PRL
Sbjct: 473 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRL 501

BLAST of Bhi01G002192 vs. NCBI nr
Match: KAA0053469.1 (cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYK19885.1 cytochrome P450 71A1-like [Cucumis melo var. makuwa])

HSP 1 Score: 709.9 bits (1831), Expect = 1.5e-200
Identity = 349/454 (76.87%), Postives = 399/454 (87.89%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKT DL FV RPK A GKY TYNY
Sbjct: 53  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNY 112

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS +RLDS EYIRK+EMNA L+EIYK SC G  I++K
Sbjct: 113 SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYK-SC-GEVIKIK 172

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 173 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 232

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHNERRK +EDYVAKDMVDVLLQL DDP+LEVK
Sbjct: 233 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVK 292

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF+KA EELDRVIG+ERWVEEKD
Sbjct: 293 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKD 352

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I KGTRV VNVW IGRDPTVW
Sbjct: 353 IINLPYIDAIAKETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVW 412

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENP EF+P+RF+ KN+D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGF+W+
Sbjct: 413 ENPLEFKPERFMGKNIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWK 472

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           L  +M NEDLNMDEVFGL+ P+KFPL  +A+PRL
Sbjct: 473 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRL 501

BLAST of Bhi01G002192 vs. ExPASy TrEMBL
Match: A0A6J1DF57 (cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111020252 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.4e-201
Identity = 345/461 (74.84%), Postives = 404/461 (87.64%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLS+KYG IM LRFGSFPVVVGSSVEMAK+FLKTHDLTFV RPK A GKY TYNY
Sbjct: 54  HQSIHQLSRKYGSIMHLRFGSFPVVVGSSVEMAKIFLKTHDLTFVSRPKTAAGKYTTYNY 113

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS KRLDS EYIR++EM+A L++IY+ SC G  I++K
Sbjct: 114 SDITWSQYGPYWRQARKMCLMELFSAKRLDSYEYIRREEMSALLRDIYR-SC-GRAIQVK 173

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S+++IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 174 DYLSTVSLNVISRMVLGKKY--TDESENAIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 233

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGY+KRMKAL+KK D FLEH+LDEHNERRK I+DYVAKDMVDVLLQL DDP+LEVK
Sbjct: 234 FLDLQGYIKRMKALSKKFDRFLEHVLDEHNERRKGIKDYVAKDMVDVLLQLADDPDLEVK 293

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF KA EELDRVIGKERWVEEKD
Sbjct: 294 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFIKATEELDRVIGKERWVEEKD 353

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I K TR++VNVW IGRDP VW
Sbjct: 354 ITNLPYIDAIAKETMRLHPVAPMLVPRMSREDCKIAGYDIAKDTRILVNVWTIGRDPEVW 413

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENPNEF P+RF+ K++D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGFSW+
Sbjct: 414 ENPNEFNPERFIGKSIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFSWK 473

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           L  +M  EDLNM+E+FGL+ P+KFPL+A+A+PRLP H Y +
Sbjct: 474 LGGDMRKEDLNMEEIFGLSTPKKFPLVAVAEPRLPPHLYSM 509

BLAST of Bhi01G002192 vs. ExPASy TrEMBL
Match: A0A1S3CCA6 (cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103499163 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 3.2e-201
Identity = 350/454 (77.09%), Postives = 399/454 (87.89%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKT DL FV RPK A GKY TYNY
Sbjct: 53  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNY 112

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS +RLDS EYIRK+EMNA L+EIYK SC G  I++K
Sbjct: 113 SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYK-SC-GEVIKIK 172

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 173 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 232

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHNERRK +EDYVAKDMVDVLLQL DDP+LEVK
Sbjct: 233 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVK 292

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF+KA EELDRVIGKERWVEEKD
Sbjct: 293 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGKERWVEEKD 352

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I KGTRV VNVW IGRDPTVW
Sbjct: 353 IINLPYIDAIAKETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVW 412

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENP EF+P+RF+ KN+D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGF+W+
Sbjct: 413 ENPLEFKPERFMGKNIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWK 472

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           L  +M NEDLNMDEVFGL+ P+KFPL  +A+PRL
Sbjct: 473 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRL 501

BLAST of Bhi01G002192 vs. ExPASy TrEMBL
Match: A0A5D3D8H9 (Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1267G00050 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 7.1e-201
Identity = 349/454 (76.87%), Postives = 399/454 (87.89%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIHQLSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKT DL FV RPK A GKY TYNY
Sbjct: 53  HQSIHQLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTQDLNFVSRPKTAAGKYTTYNY 112

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS +RLDS EYIRK+EMNA L+EIYK SC G  I++K
Sbjct: 113 SDITWSQYGPYWRQARKMCLMELFSARRLDSYEYIRKEEMNALLREIYK-SC-GEVIKIK 172

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 173 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 232

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHNERRK +EDYVAKDMVDVLLQL DDP+LEVK
Sbjct: 233 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNERRKGVEDYVAKDMVDVLLQLADDPDLEVK 292

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELLKKPEIF+KA EELDRVIG+ERWVEEKD
Sbjct: 293 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLKKPEIFNKAREELDRVIGRERWVEEKD 352

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+ RE C++ GY+I KGTRV VNVW IGRDPTVW
Sbjct: 353 IINLPYIDAIAKETMRLHPVAPMLVPRMTREDCQIAGYDIVKGTRVFVNVWTIGRDPTVW 412

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENP EF+P+RF+ KN+D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGF+W+
Sbjct: 413 ENPLEFKPERFMGKNIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWK 472

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRL 469
           L  +M NEDLNMDEVFGL+ P+KFPL  +A+PRL
Sbjct: 473 LSGDMKNEDLNMDEVFGLSTPKKFPLDVVAEPRL 501

BLAST of Bhi01G002192 vs. ExPASy TrEMBL
Match: A0A6J1IRR8 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 6.0e-200
Identity = 342/461 (74.19%), Postives = 400/461 (86.77%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIH LSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKTHDL FV RPK A GKY TYNY
Sbjct: 58  HQSIHHLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNY 117

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS KRLDS EYIR++EMNA L+++YK SC G  + +K
Sbjct: 118 SDITWSQYGPYWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYK-SC-GEVMRVK 177

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 178 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 237

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHN RR+ ++DYVAKDMVDVLLQL DDP+LEVK
Sbjct: 238 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNARREGVKDYVAKDMVDVLLQLADDPHLEVK 297

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELL+KPEI +KA EELDRVIGKERWVEEKD
Sbjct: 298 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKD 357

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           M  L YI+AIAKETMRLHP AP+LVPR+CRE C++ GY+I KGTRV+VNVW IGRDP VW
Sbjct: 358 MASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIVKGTRVLVNVWTIGRDPAVW 417

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENP+EF P+RF+ K++D+KG ++FE LPFGSGRRMCPGY+LG KVI ++LANLLHGF+W 
Sbjct: 418 ENPSEFSPERFMGKSIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQTSLANLLHGFTWS 477

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           L ++M  EDLNMDE+FGL+ P+KFPL  +A+PRLP H Y +
Sbjct: 478 LGEDMKKEDLNMDEIFGLSTPKKFPLDVVAQPRLPPHLYSI 513

BLAST of Bhi01G002192 vs. ExPASy TrEMBL
Match: A0A6J1GXG6 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 6.0e-200
Identity = 343/461 (74.40%), Postives = 400/461 (86.77%), Query Frame = 0

Query: 15  HQSIHQLSKKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNY 74
           HQSIH LSKKYGPIM LRFGSFPVVVGSSVEMAK+FLKTHDL FV RPK A GKY TYNY
Sbjct: 58  HQSIHHLSKKYGPIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNY 117

Query: 75  SNITWSQYGPYWRQARKMFMMELFSPKRLDSQEYIRKQEMNAFLQEIYKASCQGSRIELK 134
           S+ITWSQYGPYWRQARKM +MELFS KRLDS EYIR++EMNA L+++Y+ SC G  I++K
Sbjct: 118 SDITWSQYGPYWRQARKMCLMELFSAKRLDSYEYIRREEMNALLRDVYR-SC-GDVIKVK 177

Query: 135 CYLFNMTLNVISQMALGKKYLSLDQSKDSIVSPNEFKDMMDELFLLSGVLNIGDWIPWID 194
            YL  ++LNVIS+M LGKKY   D+S++ IVSP+EFK M+DELFLLSGVLNIGD IPWID
Sbjct: 178 DYLSTVSLNVISRMVLGKKY--TDESENGIVSPDEFKKMLDELFLLSGVLNIGDSIPWID 237

Query: 195 FLDLQGYVKRMKALNKKLDGFLEHILDEHNERRKRIEDYVAKDMVDVLLQLVDDPNLEVK 254
           FLDLQGYVKRMKAL+KK D FLEH+LDEHN RR+ ++DYVA+DMVDVLLQL DDP+LEVK
Sbjct: 238 FLDLQGYVKRMKALSKKFDRFLEHVLDEHNARREGVKDYVAQDMVDVLLQLADDPDLEVK 297

Query: 255 LERHGVKALIQDIISGGTDTSVLTVEWAMSELLKKPEIFDKAIEELDRVIGKERWVEEKD 314
           LERHGVKA  QD+I+GGT++S +TVEWAMSELL+KPEI +KA EELDRVIGKERWVEEKD
Sbjct: 298 LERHGVKAFTQDLIAGGTESSAVTVEWAMSELLRKPEILNKAREELDRVIGKERWVEEKD 357

Query: 315 MEKLAYIEAIAKETMRLHPAAPLLVPRLCREACEVGGYNINKGTRVVVNVWAIGRDPTVW 374
           +  L YI+AIAKETMRLHP AP+LVPR+CRE C++ GY+I KGTRV+VNVW IGRDP VW
Sbjct: 358 IASLPYIDAIAKETMRLHPVAPMLVPRMCREDCQIAGYDIAKGTRVLVNVWTIGRDPAVW 417

Query: 375 ENPNEFQPQRFVEKNMDIKGLRNFEFLPFGSGRRMCPGYNLGFKVILSTLANLLHGFSWR 434
           ENPNEF P+RF+ K++D+KG ++FE LPFGSGRRMCPGY+LG KVI S+LANLLHGF+W 
Sbjct: 418 ENPNEFSPERFMGKSIDVKG-QDFELLPFGSGRRMCPGYSLGLKVIQSSLANLLHGFTWS 477

Query: 435 LPDNMNNEDLNMDEVFGLTVPRKFPLIALAKPRLPLHNYKL 476
           L  +M  EDLNMDEVFGL+ P+KFPL  +A+PRLP H Y +
Sbjct: 478 LGGDMKKEDLNMDEVFGLSTPKKFPLDVVAQPRLPPHLYSI 513

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G07990.16.1e-9639.10Cytochrome P450 superfamily protein [more]
AT4G12300.15.0e-9039.96cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G22710.11.5e-8937.58cytochrome P450, family 706, subfamily A, polypeptide 2 [more]
AT3G26330.13.2e-8939.31cytochrome P450, family 71, subfamily B, polypeptide 37 [more]
AT4G22690.15.5e-8937.36cytochrome P450, family 706, subfamily A, polypeptide 1 [more]
Match NameE-valueIdentityDescription
A0A1D6F9Y91.7e-11946.56Trimethyltridecatetraene synthase OS=Zea mays OX=4577 GN=CYP92C6 PE=1 SV=1[more]
A0A1D6HSP44.0e-11644.92Dimethylnonatriene synthase OS=Zea mays OX=4577 GN=CYP92C5 PE=1 SV=1[more]
P244651.2e-10442.48Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q5V61.3e-10341.58Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
A0A4D6Q4152.3e-10039.87Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Match NameE-valueIdentityDescription
XP_038904918.17.9e-271100.00LOW QUALITY PROTEIN: trimethyltridecatetraene synthase-like [Benincasa hispida][more]
XP_038877760.12.7e-20274.84trimethyltridecatetraene synthase-like [Benincasa hispida][more]
XP_022152553.15.0e-20174.84cytochrome P450 71A1-like [Momordica charantia][more]
XP_008460308.16.6e-20177.09PREDICTED: cytochrome P450 71A1-like [Cucumis melo][more]
KAA0053469.11.5e-20076.87cytochrome P450 71A1-like [Cucumis melo var. makuwa] >TYK19885.1 cytochrome P450... [more]
Match NameE-valueIdentityDescription
A0A6J1DF572.4e-20174.84cytochrome P450 71A1-like OS=Momordica charantia OX=3673 GN=LOC111020252 PE=3 SV... [more]
A0A1S3CCA63.2e-20177.09cytochrome P450 71A1-like OS=Cucumis melo OX=3656 GN=LOC103499163 PE=3 SV=1[more]
A0A5D3D8H97.1e-20176.87Cytochrome P450 71A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1IRR86.0e-20074.19cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1[more]
A0A6J1GXG66.0e-20074.40cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111458046 PE=3 SV=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 410..433
score: 27.91
coord: 259..276
score: 25.28
coord: 400..410
score: 58.45
coord: 140..158
score: 26.14
coord: 322..340
score: 37.08
coord: 22..41
score: 43.1
coord: 279..305
score: 39.56
coord: 363..387
score: 38.12
IPR001128Cytochrome P450PRINTSPR00385P450coord: 270..287
score: 31.34
coord: 323..334
score: 38.28
coord: 401..410
score: 59.44
IPR001128Cytochrome P450PFAMPF00067p450coord: 14..451
e-value: 2.8E-94
score: 316.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 12..470
e-value: 5.8E-114
score: 383.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 10..467
NoneNo IPR availablePANTHERPTHR24281STEROID 21-HYDROXYLASE-RELATEDcoord: 14..466
NoneNo IPR availablePANTHERPTHR24281:SF126CYTOCHROME P450 71A1-LIKEcoord: 14..466
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 403..412

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M002192Bhi01M002192mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen