Bhi01G001811 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G001811
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Subtilisin-like protease
Location	chr1: 57228522 .. 57229352 (+)
RNA-Seq Expression	Bhi01G001811
Synteny	Bhi01G001811

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCAGTTCGTTCATGAGAAATGTCAGCTACAGAGGCCTCGGGGGTGAGACTGCAAGGGGCAGAACACCAGCGGCGCGGTTGGCAGTATACAAGGCATGTTGGAGCTTGTCCGGTGGTGGTGGCCAGTGTGCGGCGGCGGACGTATTGAAGGCGGGGATTGAACGACGCCATACACGACGGCGTTGATGTATTGTCTCTGTGTTTAGGGAAGAGTGCTCCTCCGTTTCCGGAGGTTGATGACGAGAACGTAGTTGCGATTGGGTCGTTTCGGGCAAACTTGAGGAATATTTCGGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGGTGAACACTTCACCTTGGATTTTGAATGTTGCTGCAAGTTCTATGGATAGAACTTTTTTGGGCTCTATTACTTTGGGAAACAACAACACTTTTTTGGTAAGAGATAGGCAAATGCTTCCAATTTAGAGTCCACTTGAAACTACTTTTTCTACCATGCTTGGAAGTGATTGTAGAATGAGCAAAATTACTTTTGGTTTTGATGGTAGAAAATTAAAAACAAACATTAAAATTATTATATGAAAATCACACAAAAAAGTACTTCAAATATACTTTAAAAGTATTTTTCTGAAATAATTAGCAAGTCACTCTAAACAAGTTAACCGCAAATTTTCATCGAATCTTATTTTTATACCAAAGTGAAACCTTATTCACGAGAAAGGAGATTGGATATAGTGGCTTGGTACATCCAGGATCCAAGTTGAATCCCAATATTGCAGGGTTAG

mRNA sequence

ATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCAGTTCGTTCATGAGAAATGTCAGCTACAGAGGCCTCGGGGGTGAGACTGCAAGGGGCAGAACACCAGCGGCGCGGTTGGCAGTATACAAGGCATGTTGGAGCTTGTCCGGTGGTGGTGGCCAGTGGAAGAGTGCTCCTCCGTTTCCGGAGGTTGATGACGAGAACGTAGTTGCGATTGGGTCGTTTCGGGCAAACTTGAGGAATATTTCGGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGGTGAACACTTCACCTTGGATTTTGAATGTTGCTGCAAGTTCTATGGATAGAACTTTTTTGGGCTCTATTACTTTGGGAAACAACAACACTTTTTTGGGTTAG

Coding sequence (CDS)

ATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCAGTTCGTTCATGAGAAATGTCAGCTACAGAGGCCTCGGGGGTGAGACTGCAAGGGGCAGAACACCAGCGGCGCGGTTGGCAGTATACAAGGCATGTTGGAGCTTGTCCGGTGGTGGTGGCCAGTGGAAGAGTGCTCCTCCGTTTCCGGAGGTTGATGACGAGAACGTAGTTGCGATTGGGTCGTTTCGGGCAAACTTGAGGAATATTTCGGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGGTGAACACTTCACCTTGGATTTTGAATGTTGCTGCAAGTTCTATGGATAGAACTTTTTTGGGCTCTATTACTTTGGGAAACAACAACACTTTTTTGGGTTAG

Protein sequence

MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQWKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGNDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG

Homology

[Close]

BLAST of Bhi01G001811 vs. TAIR 10
Match: AT1G32970.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 1.8e-32
Identity = 74/161 (45.96%), Postives = 98/161 (60.87%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD +GHGT  A+TAA SF+ + +Y GLG  TARG  P AR+A+YKACW L  G    
Sbjct: 171 ISPRDFDGHGTHVAATAAGSFVPDTNYLGLGRGTARGGAPRARIAMYKACWHLVTGATTC 230

Query: 61  KSA-------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
            +A                         P FPEVD ++ VA+G+F A  + I VVC+ GN
Sbjct: 231 SAADLVKAIDEAIHDGVDVLSISNGFSVPLFPEVDTQDGVAVGAFHAVAKGIPVVCAGGN 290

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
            GPS++T+ NT+PWI+ VAA++ DR+F   ITLGNN T +G
Sbjct: 291 AGPSSQTISNTAPWIITVAATTQDRSFPTFITLGNNVTVVG 331

[Close]

BLAST of Bhi01G001811 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 2.0e-31
Identity = 73/162 (45.06%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD +GHGT  A+ A  S++ ++SY+GL G T RG  P AR+A+YKACW L       
Sbjct: 210 ISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINT 269

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            S+                          P FPE D   V+A G+F A L+ I+VVCS G
Sbjct: 270 CSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGG 329

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GP+A+TV NT+PWIL VAA+++DR+F   ITLGNN   LG
Sbjct: 330 NSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG 371

[Close]

BLAST of Bhi01G001811 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 2.0e-31
Identity = 72/160 (45.00%), Postives = 94/160 (58.75%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPR  NGHGT  A+ A  S++ N SY+GL G T RG  P AR+AVYK CW L       
Sbjct: 202 ISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAAC 261

Query: 61  KSA------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGND 120
            SA                        P +PE D  + +A G+F A L+ I+VVC+AGN 
Sbjct: 262 SSADILKAMDEAIHDGVDVLSLSLGFEPLYPETDVRDGIATGAFHAVLKGITVVCAAGNA 321

Query: 121 GPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           GP+A+TV NT+PWIL VAA+++DR+F+  +TLGNN   LG
Sbjct: 322 GPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILG 361

[Close]

BLAST of Bhi01G001811 vs. TAIR 10
Match: AT4G10530.1 (Subtilase family protein )

HSP 1 Score: 132.5 bits (332), Expect = 2.6e-31
Identity = 72/157 (45.86%), Postives = 90/157 (57.32%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRD NGHGT  AST   SF+ NVSY GLG  TARG  P   +AVYKACW   G  G  
Sbjct: 210 LSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKACWVQRGCSGAD 269

Query: 61  --------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGNDGPS 120
                                 S P FPE D   + ++G+F A  + I VV +A N GP+
Sbjct: 270 VLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPVVAAASNAGPT 329

Query: 121 AETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           A+T+ N +PW+L VAA++ DR+F  +ITLGNN T LG
Sbjct: 330 AQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILG 366

[Close]

BLAST of Bhi01G001811 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 125.6 bits (314), Expect = 3.1e-29
Identity = 69/162 (42.59%), Postives = 92/162 (56.79%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +S RD  GHGT TAS A  SF+ N+SY+GL G   RG  P AR+A+YKACW +   G   
Sbjct: 209 ISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQLGAVA 268

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            S+                          P +PE D  + +A G+F A  + I VVC+ G
Sbjct: 269 CSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIVVCAGG 328

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GP+A+TV+NT+PWI+ VAA+++DR+F   ITLGN    LG
Sbjct: 329 NSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILG 370

[Close]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot
Match: Q9MAP4 (Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-31
Identity = 74/161 (45.96%), Postives = 98/161 (60.87%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD +GHGT  A+TAA SF+ + +Y GLG  TARG  P AR+A+YKACW L  G    
Sbjct: 171 ISPRDFDGHGTHVAATAAGSFVPDTNYLGLGRGTARGGAPRARIAMYKACWHLVTGATTC 230

Query: 61  KSA-------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
            +A                         P FPEVD ++ VA+G+F A  + I VVC+ GN
Sbjct: 231 SAADLVKAIDEAIHDGVDVLSISNGFSVPLFPEVDTQDGVAVGAFHAVAKGIPVVCAGGN 290

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
            GPS++T+ NT+PWI+ VAA++ DR+F   ITLGNN T +G
Sbjct: 291 AGPSSQTISNTAPWIITVAATTQDRSFPTFITLGNNVTVVG 331

[Close]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 2.8e-30
Identity = 72/160 (45.00%), Postives = 94/160 (58.75%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPR  NGHGT  A+ A  S++ N SY+GL G T RG  P AR+AVYK CW L       
Sbjct: 214 ISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAAC 273

Query: 61  KSA------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGND 120
            SA                        P +PE D  + +A G+F A L+ I+VVC+AGN 
Sbjct: 274 SSADILKAMDEAIHDGVDVLSLSLGFEPLYPETDVRDGIATGAFHAVLKGITVVCAAGNA 333

Query: 121 GPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           GP+A+TV NT+PWIL VAA+++DR+F+  +TLGNN   LG
Sbjct: 334 GPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILG 373

[Close]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.8e-30
Identity = 73/162 (45.06%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD +GHGT  A+ A  S++ ++SY+GL G T RG  P AR+A+YKACW L       
Sbjct: 210 ISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINT 269

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            S+                          P FPE D   V+A G+F A L+ I+VVCS G
Sbjct: 270 CSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGG 329

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GP+A+TV NT+PWIL VAA+++DR+F   ITLGNN   LG
Sbjct: 330 NSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG 371

[Close]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 3.6e-30
Identity = 72/157 (45.86%), Postives = 90/157 (57.32%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRD NGHGT  AST   SF+ NVSY GLG  TARG  P   +AVYKACW   G  G  
Sbjct: 210 LSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKACWVQRGCSGAD 269

Query: 61  --------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGNDGPS 120
                                 S P FPE D   + ++G+F A  + I VV +A N GP+
Sbjct: 270 VLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPVVAAASNAGPT 329

Query: 121 AETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           A+T+ N +PW+L VAA++ DR+F  +ITLGNN T LG
Sbjct: 330 AQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILG 366

[Close]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.4e-28
Identity = 69/162 (42.59%), Postives = 92/162 (56.79%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +S RD  GHGT TAS A  SF+ N+SY+GL G   RG  P AR+A+YKACW +   G   
Sbjct: 209 ISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQLGAVA 268

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            S+                          P +PE D  + +A G+F A  + I VVC+ G
Sbjct: 269 CSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIVVCAGG 328

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GP+A+TV+NT+PWI+ VAA+++DR+F   ITLGN    LG
Sbjct: 329 NSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILG 370

[Close]

BLAST of Bhi01G001811 vs. NCBI nr
Match: XP_038888301.1 (LOW QUALITY PROTEIN: subtilisin-like protease SBT3.10 [Benincasa hispida])

HSP 1 Score: 253.1 bits (645), Expect = 1.4e-63
Identity = 134/161 (83.23%), Postives = 134/161 (83.23%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ 
Sbjct: 678 MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQC 737

Query: 61  -------------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
                                     KSAPPFPEVDDENVVAIGSFRANLRNISVVCSAG
Sbjct: 738 AAADVLKAGLNDAIHDGVDVLSLCLGKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAG 797

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFL 136
           NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFL
Sbjct: 798 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFL 838

[Close]

BLAST of Bhi01G001811 vs. NCBI nr
Match: XP_022136960.1 (subtilisin-like protease SBT3.5 isoform X2 [Momordica charantia])

HSP 1 Score: 205.3 bits (521), Expect = 3.3e-49
Identity = 110/161 (68.32%), Postives = 117/161 (72.67%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRDSNGHGTQTASTAA SF+ NVSYRGL G T RG  P ARLA+YKACWSL  GGGQ 
Sbjct: 206 ISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSGGGQC 265

Query: 61  ------------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
                                    KS P FPEVD+ENVVAIGSF A  RNI VVCSAGN
Sbjct: 266 AAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVCSAGN 325

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           DGPS+ETV+NTSPWILNVAASSMDRTFL +I LGNNNTFLG
Sbjct: 326 DGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLG 366

[Close]

BLAST of Bhi01G001811 vs. NCBI nr
Match: XP_022136958.1 (subtilisin-like protease SBT3.5 isoform X1 [Momordica charantia])

HSP 1 Score: 203.4 bits (516), Expect = 1.3e-48
Identity = 109/160 (68.12%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRDSNGHGTQTASTAA SF+ NVSYRGL G T RG  P ARLA+YKACWSL  GGGQ 
Sbjct: 206 ISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSGGGQC 265

Query: 61  ------------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
                                    KS P FPEVD+ENVVAIGSF A  RNI VVCSAGN
Sbjct: 266 AAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVCSAGN 325

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFL 136
           DGPS+ETV+NTSPWILNVAASSMDRTFL +I LGNNNTFL
Sbjct: 326 DGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFL 365

[Close]

BLAST of Bhi01G001811 vs. NCBI nr
Match: KAG5255320.1 (subtilase family protein [Salix suchowensis])

HSP 1 Score: 163.7 bits (413), Expect = 1.1e-36
Identity = 88/162 (54.32%), Postives = 106/162 (65.43%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD+NGHGT TASTAA  F+ NVSY+GLG  T RG  P ARLA+YK CW++ GG   W
Sbjct: 215 LSPRDANGHGTHTASTAAGGFVENVSYKGLGLGTVRGGAPRARLAIYKVCWNVLGG---W 274

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            SA                          P F + D+ + +AIGSF A  + I+VVCSAG
Sbjct: 275 CSAADVLKAFDEAIHDGVDVLSLSLGYPPPLFSDTDERDGIAIGSFHAVTKGITVVCSAG 334

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GPS++TV+NTSPWILNVAAS++DR FL  ITLGNN TFLG
Sbjct: 335 NAGPSSQTVLNTSPWILNVAASTIDRAFLTPITLGNNKTFLG 373

[Close]

BLAST of Bhi01G001811 vs. NCBI nr
Match: XP_024026852.1 (subtilisin-like protease SBT3.6 isoform X1 [Morus notabilis])

HSP 1 Score: 161.0 bits (406), Expect = 7.2e-36
Identity = 88/161 (54.66%), Postives = 104/161 (64.60%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD+NGHGT TASTAA SF+ NVSY+GL G  ARG  P AR+AVYK CW+L   GGQ 
Sbjct: 214 LSPRDANGHGTHTASTAAGSFVGNVSYKGLAGGNARGGAPKARVAVYKVCWNLL--GGQC 273

Query: 61  KSA-------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
            SA                         P + +VD+ + +A GSF A    ISVVC AGN
Sbjct: 274 SSADILKAFDEAIHDGVDVLSLSIGFGIPLYADVDERDGIATGSFHAVANGISVVCGAGN 333

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           DGPSA+TV NT+PWIL VAA+S+DR FL SITLGNN T +G
Sbjct: 334 DGPSAQTVQNTAPWILTVAANSIDRAFLASITLGNNKTLVG 372

[Close]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL
Match: A0A6J1C5D1 (subtilisin-like protease SBT3.5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.6e-49
Identity = 110/161 (68.32%), Postives = 117/161 (72.67%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRDSNGHGTQTASTAA SF+ NVSYRGL G T RG  P ARLA+YKACWSL  GGGQ 
Sbjct: 206 ISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSGGGQC 265

Query: 61  ------------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
                                    KS P FPEVD+ENVVAIGSF A  RNI VVCSAGN
Sbjct: 266 AAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVCSAGN 325

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           DGPS+ETV+NTSPWILNVAASSMDRTFL +I LGNNNTFLG
Sbjct: 326 DGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLG 366

[Close]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL
Match: A0A6J1C8Y6 (subtilisin-like protease SBT3.5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 6.2e-49
Identity = 109/160 (68.12%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQ- 60
           +SPRDSNGHGTQTASTAA SF+ NVSYRGL G T RG  P ARLA+YKACWSL  GGGQ 
Sbjct: 206 ISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSGGGQC 265

Query: 61  ------------------------WKSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
                                    KS P FPEVD+ENVVAIGSF A  RNI VVCSAGN
Sbjct: 266 AAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVCSAGN 325

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFL 136
           DGPS+ETV+NTSPWILNVAASSMDRTFL +I LGNNNTFL
Sbjct: 326 DGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFL 365

[Close]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL
Match: W9RY09 (Subtilisin-like protease OS=Morus notabilis OX=981085 GN=L484_024115 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.5e-36
Identity = 88/161 (54.66%), Postives = 104/161 (64.60%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD+NGHGT TASTAA SF+ NVSY+GL G  ARG  P AR+AVYK CW+L   GGQ 
Sbjct: 242 LSPRDANGHGTHTASTAAGSFVGNVSYKGLAGGNARGGAPKARVAVYKVCWNLL--GGQC 301

Query: 61  KSA-------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAGN 120
            SA                         P + +VD+ + +A GSF A    ISVVC AGN
Sbjct: 302 SSADILKAFDEAIHDGVDVLSLSIGFGIPLYADVDERDGIATGSFHAVANGISVVCGAGN 361

Query: 121 DGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           DGPSA+TV NT+PWIL VAA+S+DR FL SITLGNN T +G
Sbjct: 362 DGPSAQTVQNTAPWILTVAANSIDRAFLASITLGNNKTLVG 400

[Close]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL
Match: A0A6N2N4F0 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS463159 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.5e-36
Identity = 87/162 (53.70%), Postives = 105/162 (64.81%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD+NGHGT TASTAA  F+ NVSY+G G  T RG  P ARLA+YK CW++ GG   W
Sbjct: 825 LSPRDANGHGTHTASTAAGGFVENVSYKGPGLGTVRGGAPRARLAIYKVCWNVLGG---W 884

Query: 61  KSA--------------------------PPFPEVDDENVVAIGSFRANLRNISVVCSAG 120
            SA                          P F + D+ + +AIGSF A  + I+VVCSAG
Sbjct: 885 CSAADVLKAFDEAIHDGVDVLSLSLGYPPPLFSDTDERDGIAIGSFHAVTKGITVVCSAG 944

Query: 121 NDGPSAETVVNTSPWILNVAASSMDRTFLGSITLGNNNTFLG 137
           N GPS++TV+NTSPWILNVAAS++DR FL  ITLGNN TFLG
Sbjct: 945 NAGPSSQTVLNTSPWILNVAASTIDRAFLTPITLGNNKTFLG 983

[Close]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL
Match: A0A2N9F3Z7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS9602 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.0e-35
Identity = 81/136 (59.56%), Postives = 97/136 (71.32%), Query Frame = 0


Query: 1   MSPRDSNGHGTQTASTAASSFMRNVSYRGLGGETARGRTPAARLAVYKACWSLSGGGGQW 60
           +SPRD+NGHGT TASTA  SF+ NVSY+GLG  + RG  P A LA+YK CW + GG    
Sbjct: 150 LSPRDANGHGTHTASTAGGSFVGNVSYKGLGSGSVRGGAPHAHLAIYKVCWDVLGG---L 209

Query: 61  KSAPPFPEVDDENVVAIGSFRANLRNISVVCSAGNDGPSAETVVNTSPWILNVAASSMDR 120
            S P F +VD+ + +A GSF A  R I+VVC+A N GPSA TV N++PWIL VAAS+MDR
Sbjct: 210 SSIPRFSDVDERDDIATGSFHAVARGITVVCAAANYGPSAYTVQNSAPWILTVAASTMDR 269

Query: 121 TFLGSITLGNNNTFLG 137
            FL  ITLGNN T LG
Sbjct: 270 AFLSPITLGNNKTLLG 282

The following BLAST results are available for this feature:

BLAST of Bhi01G001811 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32970.1	1.8e-32	45.96	Subtilisin-like serine endopeptidase family protein	[more]
AT4G10540.1	2.0e-31	45.06	Subtilase family protein	[more]
AT4G10510.1	2.0e-31	45.00	Subtilase family protein	[more]
AT4G10530.1	2.6e-31	45.86	Subtilase family protein	[more]
AT1G32940.1	3.1e-29	42.59	Subtilase family protein	[more]

BLAST of Bhi01G001811 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MAP4	2.5e-31	45.96	Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 S...	[more]
Q9SZY2	2.8e-30	45.00	Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 S...	[more]
Q9SZY3	2.8e-30	45.06	Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S...	[more]
Q9ZSB1	3.6e-30	45.86	Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3...	[more]
Q9MAP7	4.4e-28	42.59	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]

BLAST of Bhi01G001811 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888301.1	1.4e-63	83.23	LOW QUALITY PROTEIN: subtilisin-like protease SBT3.10 [Benincasa hispida]	[more]
XP_022136960.1	3.3e-49	68.32	subtilisin-like protease SBT3.5 isoform X2 [Momordica charantia]	[more]
XP_022136958.1	1.3e-48	68.13	subtilisin-like protease SBT3.5 isoform X1 [Momordica charantia]	[more]
KAG5255320.1	1.1e-36	54.32	subtilase family protein [Salix suchowensis]	[more]
XP_024026852.1	7.2e-36	54.66	subtilisin-like protease SBT3.6 isoform X1 [Morus notabilis]	[more]

BLAST of Bhi01G001811 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5D1	1.6e-49	68.32	subtilisin-like protease SBT3.5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1C8Y6	6.2e-49	68.13	subtilisin-like protease SBT3.5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
W9RY09	3.5e-36	54.66	Subtilisin-like protease OS=Morus notabilis OX=981085 GN=L484_024115 PE=3 SV=1	[more]
A0A6N2N4F0	3.5e-36	53.70	Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS463159 PE=3 SV=...	[more]
A0A2N9F3Z7	1.0e-35	59.56	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS9602 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 2..118 e-value: 9.1E-34 score: 119.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 2..132
None	No IPR available	GENE3D	3.50.30.30		coord: 119..135 e-value: 9.1E-34 score: 119.0
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 2..56
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 62..134
None	No IPR available	PANTHER	PTHR43806:SF50	SUBTILISIN-LIKE PROTEASE SBT3.1-RELATED	coord: 62..134 coord: 2..56

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M001811	Bhi01M001811	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity