Bhi01G001621 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001621
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr1: 48319484 .. 48324131 (-)
RNA-Seq ExpressionBhi01G001621
SyntenyBhi01G001621
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATAATACCCGTACTGAAGATTACATATAACGCTACCAAATTTTGCTGTTTTTGGAACTGGGCAGTCGGGATTTCCCTTGTATTCCACTCTTTTGGGCCTTCTTCTCTATATTTCTGATGAATGAACGGGGTGTTGGATGACCCATACGCAATTCATTTTGTACTCGTCAACTGTTGACTACTACATAATTGAGGTGAATTGAATTCCACTTCTCTGTCTTTGATTTTTCTTTATAATCCTGCAACGATTCTTCTTATTTGCCCACGAATTGAAGACCCATTTGCTTTCAAAGATGGAGAGAGCGACGAAGAACGTCTTACTTTCTTCTAATGGGGATGACATCTCCAAGAATCTGGCTATTCATCTTGCCAAAAGGGGTTGCAGGTTCAGCATCCAACTTATAACTTAGTGGTTTTATGTGTATATTTCCTGAGGAAATTGAGAGAAATGGAGGACGTTCATCGAACTTTTCGTTATTGGGTTTCTCTTTTCTGTTTTGTTTGCCTCTTTAATGCATTTTTTATTCACAGTTGTTAATCATGTATGCATCTTTTTTCATGGCTATTTTCAGACTGGTTTTGATTGGAAATGAAAAGGTTCTTCAGAGCATGAGCAAGGTGATGGCGGAATCCTTAAAGGGCGTCCTGCCAATTGAGGTTGTTGGGTTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATAAGGCTGTGAACAGGGCATGTAGTATTTTGGGAACTTTGGATGCTTTTGCCCATGCCTATTCTTATGAAGGTACAGCCCATTTTTCTCTTGCCTATGTACTTTCTTTTTTTATATAACTTGACTGTGATGTTGTGAGTTTGTTATATAGTTTTTGGTTTGATTATTTTAGGTTCTTTGTATTAGTTAGTTGTTGATTCCTTGTTTGGGCTTGGTTTGATGTTGTGGGGAAGATTGAATAGAGATTTTCTTTGTGTATTTTTGGAAAGAGGTTAGGCCACACCATCCTGACTTTCCTGAATTATCTGCTCATAGTTTTTGGTGAACTCAGTGACTGTTTATAGATATTGGTTTACTTCACTGAGCTTAGAACTGAAGCTAGAAGGGTTATTCTGAGAGTTATTGGTTTTAGTGTGGATTCTAATTTCAAATGAGAATCCATGTTTAAAAGGCTTATTTCTTGAGGTTGACTTGAGGCTAGTTTCAAAGCTTACTCCTTCCCTTGTGTTGTAAATATAATAGGCACAAAGTCTTTTGATGTTGAGGCTTAGAATGTATATGCCTATGTAATAGTTAAAAGAATGGTGTTAAATTGTTCTCTTGAAAAAATTGCAGTTTTGTCATATTTATCATTGCGTTATTCTTGGTCTTCTTCTTCGTTTCTTATTATTATTATTATTATTATTTTTTTTTGGAAATGGAACAAGGCTTTTCATTGAAGTAATGAAATGAGTCTAATGCTCAAAGTACAAGAAATTGAAACAAAAGGAAGCTTAGCAACAAAAGAAAATACAAGAATATAAATAGACCAATACTATATCAAACCAAACAAATGTAGCTAAGAAGAAATAAATGCATTCTAATTTAAATTAATATCTTGAATAAAGTAATCAGCAAATAATTTGGAAAGACAGCACCATGAAGAAGTTTTGACTCGAGCAATCTTATATTGATCAAACCAAGGAAAAGTTTTTTATTTGTTTTTTAAAAATTCAAATTGAGGCTTATGCCTCATGCTTCCCCTCTCAAAGGAAAAAAAAAATATCATGCAAGGTGGCTTTTAGCCGTTTAAGGAATTGGAAAGAACGAAGACCTATAGAGCTTTCTGCTTGATCAATGACTTCACTTTTGTGCTGATGAACATGGAAAAGAATCTGAAATCTTAATTGTATATATAAGCTTTTTTTTTGTATCTGTATAGAATTTCTTCCCTTTTCCTGTGGCATGCTGAATTTTAATTGTTTAGTATCATTTGAATGTTTGGAAAGTTGACAAATGACATTAGAAGTCCACTCTGTTCTTCTTATGAAAAAAGTTGTACTGAAAGTAAATTGTGAATCGAAAAGCAGAACATTGTGAAAGTCTTCTTTCTTCAGGACAAAGTTCTGCAGAGCCGTCCCTTTCTCAATGAAGAACCATGGAATTACTTTCCTTTAATTTTATCTCCATAAACTTCTTCAAAATGTCTTTTGAGGGCATAGCATGCAGAAATTAAGAAAACTCCACAATGGAATTATTGCTAGCTCGATTTGAAATGAAATAGACTACATTTACAAAAAATAGGATCTTATCAGTACACCTACTGTCCTCTCTGCCGAGCCAGTTGAGATGATGCTTCTTATAGAACCAAGCCAGAATTGAAGGAAAAACAGTTGGCTTTTAAAATCGATGCCATGTATAATTGTTCGTTTATTGATATTTGCTTTACATTTTCTTTAAGATATAATACTCAGCTCTGCTATTGGATAGTGATAATAAGGATGTAGAACTTCTCTGTTAGCTATCCCACTGTAAACTGTGTTAACTGCATTGCGTTTGAAGCAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCCAAAAGATTGTTAGGATAAATTTGATGGCTTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGGGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGACTGTATCAAGGAGGAGCTGCATATGGATCATGCTTAGCAGGATTACAGCAGTTAGCTCGAGTAAGTAGTTGTCAACTTGTCATTGGTTACTGCCTCCACTGGGGATTCTTCTTTTTCTTCTTTGTTTCATTGCAGCTATTATTTTTTTGCTTCCTAGGATCTCTTTTCATGAGTGTATTTCTGCATTATTATTTGCTAGAAAGAGTTGTTCAAACCTCAGACTTCATCTTCCCAACTTGGCATCTTAGTATATTCCTTTATATAGCTTGAAGTTGTGGAATCTGTGTGACGCAGTATTTTCATTTCCATGATGTTTAACTACATTCCGGCTGATATTCGCTCATCATATCCATACCCCTCATTCGTCGATTTTCCTGTTCACTATCACCTCACATGAGGAGTACTTGAATAACAAGGCTACTTGGCATGTCAAGATGAGATGAGGGTTTAAAGAAAGGGTTGAGCTATGAGAGGGGTTACAAATAAGTCTTGCAAAAAAAGGGGAGGATTATTAGATAGATGTTAATCAAGAGCTCAAAGAGAAGATTTAAAATGCACCAAAATTTTAACATCGTCTATGAACTAGTTACTCCTATAATGTTCTCCTTTTCCTTTTCTCAACCAAATAAATCACATAATGGCGGATAAACCAGCCTTGCCGGAGAAGATTCTAAACAACTTTTTCCATCATTTCATGGATTTTCATCTCTTTACGGCCACACTAAGCAATGAAGTACCTTATATTTTTCCCCAGTCTTGGATTGTCTCACAATCCCTTCTAACAAATAAGCAAAAACTGTGCTACATACATTGTGTAGAATCATCCAAGAAACTCTGAAAATCTTTTAGAACCATATTAAACCTGAGAGTTCATTGATTTCAGAAAGATGGTGATATACTCTTGTGTATGGCCTTAATCGTAGTAGAACTAGAAAGATTTATATAGCCAACTCTAGCCCTTCAAATGAGCTTTGTTCTTATTTTTATTAAATGCTGTTATTTAGAAATCAGAACAAAAGATCTTAATCTAGTGTACCTTGAAACTAGTTCCGGATACAAGGTTTATAGTGCAATTTTTGTTGGAAACAAACTTGACCAACCTGCAAAAAAGTCTGATCTCTATTTGGATTCATAACCTGATCTGTCCTTAAAGTTCCCTCATATGATGACATCTATGATGAATTTTTCTAGACTTTAGCTCTGGACGTTGGAAAGTATAAGATCAGGGTCAATGCAATCGCCCGTGGATTGCACCTTGATGATGGATACCCTGTATCAGTGGGCAAGGAGCGAGCAAAGAAGCTGGTCAAGGAAGCAGCCCCACTGGAGAGATGGCTCGATGTCAAGGAGGATTTAGCTTCAACCGTCATCTATCTAATCAGTGACGGGTCACGGTATATGACTGGAACAACCATATTTGTTGATGGGGCACAGTCTCTGGTGAGGCCGCGGATGCGCTCATATATGTAGTTCTTATACATGGTGTTTGGACAGCCTTTGGTGAGAACAACACCCATAACCATCTTGCGTTTGTATGAACTTCTTGTTAGTTGTTTCCATGAGATAATGTTTTAAGTCCTGAATCTTTTGTTTCTGCAGGTTGGATGCGAACTGGAACTGTTGTTTTCTACCAATACTCATGAATAAATTTGAAGTTTAGTGAAAGCATGTTTGAGTTGATAGTTTCTTTAAAGCCCTTTCTCTTGGTTTATTTTCATAGTTGAAGTTAGGAATTTTCCAGAATTCCAAAGTTTATAAATAGTGATTTCAAAGTGTATGATAATCGCAAGGACACTATGTAACATAAGTTAGTAGCGCATATCGTGTTTGAAAGGAAGTTAATTAACTATTTTGTCAAGCAAATTGTGAGACAACTTCATGAATTTGAGTAATGTTCTTTGAAAGGGGAACTAGACCAACCATTATCAGAGCATGGACTTCATTTCATTTGCC

mRNA sequence

CTATAATACCCGTACTGAAGATTACATATAACGCTACCAAATTTTGCTGTTTTTGGAACTGGGCAGTCGGGATTTCCCTTGTATTCCACTCTTTTGGGCCTTCTTCTCTATATTTCTGATGAATGAACGGGGTGTTGGATGACCCATACGCAATTCATTTTGTACTCGTCAACTGTTGACTACTACATAATTGAGATGGAGAGAGCGACGAAGAACGTCTTACTTTCTTCTAATGGGGATGACATCTCCAAGAATCTGGCTATTCATCTTGCCAAAAGGGGTTGCAGACTGGTTTTGATTGGAAATGAAAAGGTTCTTCAGAGCATGAGCAAGGTGATGGCGGAATCCTTAAAGGGCGTCCTGCCAATTGAGGTTGTTGGGTTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATAAGGCTGTGAACAGGGCATGTAGTATTTTGGGAACTTTGGATGCTTTTGCCCATGCCTATTCTTATGAAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCCAAAAGATTGTTAGGATAAATTTGATGGCTTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGGGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGACTGTATCAAGGAGGAGCTGCATATGGATCATGCTTAGCAGGATTACAGCAGTTAGCTCGAACTTTAGCTCTGGACGTTGGAAAGTATAAGATCAGGGTCAATGCAATCGCCCGTGGATTGCACCTTGATGATGGATACCCTGTATCAGTGGGCAAGGAGCGAGCAAAGAAGCTGGTCAAGGAAGCAGCCCCACTGGAGAGATGGCTCGATGTCAAGGAGGATTTAGCTTCAACCGTCATCTATCTAATCAGTGACGGGTCACGGTATATGACTGGAACAACCATATTTGTTGATGGGGCACAGTCTCTGGTGAGGCCGCGGATGCGCTCATATATGTAGTTCTTATACATGGTGTTTGGACAGCCTTTGGTTGGATGCGAACTGGAACTGTTGTTTTCTACCAATACTCATGAATAAATTTGAAGTTTAGTGAAAGCATGTTTGAGTTGATAGTTTCTTTAAAGCCCTTTCTCTTGGTTTATTTTCATAGTTGAAGTTAGGAATTTTCCAGAATTCCAAAGTTTATAAATAGTGATTTCAAAGTGTATGATAATCGCAAGGACACTATGTAACATAAGTTAGTAGCGCATATCGTGTTTGAAAGGAAGTTAATTAACTATTTTGTCAAGCAAATTGTGAGACAACTTCATGAATTTGAGTAATGTTCTTTGAAAGGGGAACTAGACCAACCATTATCAGAGCATGGACTTCATTTCATTTGCC

Coding sequence (CDS)

ATGACCCATACGCAATTCATTTTGTACTCGTCAACTGTTGACTACTACATAATTGAGATGGAGAGAGCGACGAAGAACGTCTTACTTTCTTCTAATGGGGATGACATCTCCAAGAATCTGGCTATTCATCTTGCCAAAAGGGGTTGCAGACTGGTTTTGATTGGAAATGAAAAGGTTCTTCAGAGCATGAGCAAGGTGATGGCGGAATCCTTAAAGGGCGTCCTGCCAATTGAGGTTGTTGGGTTGGACATGGAAGAAGAGAGAGAGGCGGCTTTTGATAAGGCTGTGAACAGGGCATGTAGTATTTTGGGAACTTTGGATGCTTTTGCCCATGCCTATTCTTATGAAGGTCCCATACAAGATGCTTTACAACTATCTGAAGAGGAATTCCAAAAGATTGTTAGGATAAATTTGATGGCTTCATGGTTTCTGATGAAGGCTGTTTGTCGAAGAATGCGGGACCAGAAATCAGGAGGTTCAGTAATTTTTTTAACCACCTTAATTGGTGCTGAGAGGGGACTGTATCAAGGAGGAGCTGCATATGGATCATGCTTAGCAGGATTACAGCAGTTAGCTCGAACTTTAGCTCTGGACGTTGGAAAGTATAAGATCAGGGTCAATGCAATCGCCCGTGGATTGCACCTTGATGATGGATACCCTGTATCAGTGGGCAAGGAGCGAGCAAAGAAGCTGGTCAAGGAAGCAGCCCCACTGGAGAGATGGCTCGATGTCAAGGAGGATTTAGCTTCAACCGTCATCTATCTAATCAGTGACGGGTCACGGTATATGACTGGAACAACCATATTTGTTGATGGGGCACAGTCTCTGGTGAGGCCGCGGATGCGCTCATATATGTAG

Protein sequence

MTHTQFILYSSTVDYYIIEMERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEVVGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM
Homology
BLAST of Bhi01G001621 vs. TAIR 10
Match: AT3G01980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 5.9e-86
Identity = 149/266 (56.02%), Postives = 200/266 (75.19%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME   K VL++SNGD++S+N+A HLAK GC+LV++GNE  L+S+   + +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           + LDME + E AF  AV +A  + G  DAF ++Y+Y+G +QD LQ+S++EF +I +INL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           A WFL+KAV  RM+D  SGGS++F+ T+   ER LY G  AY S  A + QL R  A+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GK+KIRVN I+RGLHLDD Y  SVG++RA+KLVK+AAPL +WL+   DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

BLAST of Bhi01G001621 vs. TAIR 10
Match: AT3G01980.4 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 5.9e-86
Identity = 149/266 (56.02%), Postives = 200/266 (75.19%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME   K VL++SNGD++S+N+A HLAK GC+LV++GNE  L+S+   + +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           + LDME + E AF  AV +A  + G  DAF ++Y+Y+G +QD LQ+S++EF +I +INL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           A WFL+KAV  RM+D  SGGS++F+ T+   ER LY G  AY S  A + QL R  A+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GK+KIRVN I+RGLHLDD Y  SVG++RA+KLVK+AAPL +WL+   DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

BLAST of Bhi01G001621 vs. TAIR 10
Match: AT3G01980.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.3e-81
Identity = 149/296 (50.34%), Postives = 200/296 (67.57%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME   K VL++SNGD++S+N+A HLAK GC+LV++GNE  L+S+   + +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYE----------------------- 139
           + LDME + E AF  AV +A  + G  DAF ++Y+Y+                       
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 140 -------GPIQDALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIG 199
                  G +QD LQ+S++EF +I +INL A WFL+KAV  RM+D  SGGS++F+ T+  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

Query: 200 AERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAK 259
            ER LY G  AY S  A + QL R  A+ +GK+KIRVN I+RGLHLDD Y  SVG++RA+
Sbjct: 181 GERALYPGADAYASTSAAIHQLVRASAMSLGKHKIRVNMISRGLHLDDEYTASVGRDRAQ 240

Query: 260 KLVKEAAPLERWLDVKEDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM 286
           KLVK+AAPL +WL+   DL STVIYLISDGSR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 KLVKDAAPLGQWLNPDTDLYSTVIYLISDGSRFMTGTTVLVDGAQSLTRPRLKSYM 296

BLAST of Bhi01G001621 vs. TAIR 10
Match: AT3G01980.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 169.9 bits (429), Expect = 3.0e-42
Identity = 86/208 (41.35%), Postives = 123/208 (59.13%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME   K VL++SNGD++S+N+A HLAK GC+LV++GNE  L+S+   + +S++G  P +V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYE----------------------- 139
           + LDME + E AF  AV +A  + G  DAF ++Y+Y+                       
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 140 -------GPIQDALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIG 198
                  G +QD LQ+S++EF +I +INL A WFL+KAV  RM+D  SGGS++F+ T+  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

BLAST of Bhi01G001621 vs. TAIR 10
Match: AT1G63380.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-36
Identity = 88/258 (34.11%), Postives = 137/258 (53.10%), Query Frame = 0

Query: 21  ERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAE-SLKGVLPIEV 80
           E   K VL++     I + + + L K GC++V       +  ++ + +E +  G + ++ 
Sbjct: 16  ELKDKVVLVTGASSGIGREICLDLCKAGCKIVAAARR--VDRLNSLCSEINSFGAIGVQA 75

Query: 81  VGLDMEEEREA-AFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINL 140
           V L+++   EA    KAV  A    G +D   +     G ++ +L LSEEE+ K+ R NL
Sbjct: 76  VALELDVSSEADTIRKAVKEAWETFGKIDVLINNAGIRGNVKSSLDLSEEEWDKVFRTNL 135

Query: 141 MASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALD 200
             SW + K VC  MRD + GGSVI ++++ G  RGL +GG AY     G+  + R +A++
Sbjct: 136 TGSWLISKYVCLLMRDAERGGSVINVSSISGLHRGLLRGGLAYACSKGGVDTMTRMMAIE 195

Query: 201 VGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISD 260
           +  YKIRVN+IA G+   +       KE  KK+ ++  PL+    V   L S V YLI D
Sbjct: 196 LAVYKIRVNSIAPGIFRSEITQGLFQKEWLKKVTEKVVPLKMQQTVDPGLTSLVRYLIHD 255

Query: 261 GSRYMTGTTIFVDGAQSL 277
            S+Y+TG T  VD   +L
Sbjct: 256 SSQYVTGNTYIVDSGTTL 271

BLAST of Bhi01G001621 vs. ExPASy Swiss-Prot
Match: Q9LBG2 (Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.1e-16
Identity = 69/255 (27.06%), Postives = 119/255 (46.67%), Query Frame = 0

Query: 27  VLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEVVGLDMEE 86
           VL++  G  + +  A+ LA  G +L L+  +   + +    A  L+     EV+    + 
Sbjct: 16  VLITGGGSGLGRATAVRLAAEGAKLSLV--DVSSEGLEASKAAVLETAPDAEVLTTVADV 75

Query: 87  EREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLMASWFLMK 146
             EA  +  V       G +D F +    EG        +  EF K+V INL   +  ++
Sbjct: 76  SDEAQVEAYVTATTERFGRIDGFFNNAGIEGKQNPTESFTAAEFDKVVSINLRGVFLGLE 135

Query: 147 AVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKIRV 206
            V + MR+Q SG  ++  T  +G  RG+    + Y +   G+  L R  A++ G+Y IR+
Sbjct: 136 KVLKIMREQGSG--MVVNTASVGGIRGI-GNQSGYAAAKHGVVGLTRNSAVEYGRYGIRI 195

Query: 207 NAIARG---LHLDDGYPVSVGKERAKKLVKE---AAPLERWLDVKEDLASTVIYLISDGS 266
           NAIA G     + +     +  E  +K  +E     P +R+ +  E +A+ V +L+SD +
Sbjct: 196 NAIAPGAIWTPMVENSMKQLDPENPRKAAEEFIQVNPSKRYGEAPE-IAAVVAFLLSDDA 255

Query: 267 RYMTGTTIFVDGAQS 276
            Y+  T + +DG QS
Sbjct: 256 SYVNATVVPIDGGQS 264

BLAST of Bhi01G001621 vs. ExPASy Swiss-Prot
Match: P9WGQ2 (Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1753.1 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.3e-15
Identity = 69/252 (27.38%), Postives = 116/252 (46.03%), Query Frame = 0

Query: 25  KNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEVVGLDM 84
           K++L++     + +  A  LA  G RL L G        S  +AE + G    +   +  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGGN------SAGLAELVNGAGIDDAAVVTC 82

Query: 85  EEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLMASWFL 144
             +  A   + V  A    G LD    A S    +    +++ E+F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 145 MKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKI 204
            +A  R + +Q  GGSV+ ++++ G   G   G +AY    AG   LA+TLA + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 205 RVNAIARGLHLDDGYPVSVGKERAKKLVKEA----APLERWLDVKEDLASTVIYLISDGS 264
           RVNA+A  +            +   +  +EA     PL R+ +  ED    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 265 RYMTGTTIFVDG 273
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

BLAST of Bhi01G001621 vs. ExPASy Swiss-Prot
Match: P9WGQ3 (Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1714 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.3e-15
Identity = 69/252 (27.38%), Postives = 116/252 (46.03%), Query Frame = 0

Query: 25  KNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEVVGLDM 84
           K++L++     + +  A  LA  G RL L G        S  +AE + G    +   +  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGGN------SAGLAELVNGAGIDDAAVVTC 82

Query: 85  EEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLMASWFL 144
             +  A   + V  A    G LD    A S    +    +++ E+F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 145 MKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKI 204
            +A  R + +Q  GGSV+ ++++ G   G   G +AY    AG   LA+TLA + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 205 RVNAIARGLHLDDGYPVSVGKERAKKLVKEA----APLERWLDVKEDLASTVIYLISDGS 264
           RVNA+A  +            +   +  +EA     PL R+ +  ED    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 265 RYMTGTTIFVDG 273
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

BLAST of Bhi01G001621 vs. ExPASy Swiss-Prot
Match: P32573 (Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPS19 PE=1 SV=4)

HSP 1 Score: 80.1 bits (196), Expect = 4.5e-14
Identity = 69/231 (29.87%), Postives = 117/231 (50.65%), Query Frame = 0

Query: 48  GCRLVLIG-----NEKVLQSMSKVMAESLKGVLPIEVVGLDMEEEREAAFDKAVNRACSI 107
           GC+  ++G      E+  + +S+ +A+    VL I  V +   E+ E A  K V +    
Sbjct: 48  GCKAAIVGRDQERTEQAAKGISQ-LAKDKDAVLAIANVDVRNFEQVENAVKKTVEK---- 107

Query: 108 LGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVI 167
            G +D F  A +    + D   LS   F+ +V I+L+ S+   KA  + ++  KS GS++
Sbjct: 108 FGKID-FVIAGAAGNFVCDFANLSPNAFKSVVDIDLLGSFNTAKACLKELK--KSKGSIL 167

Query: 168 FLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYKIRVNAIARG-LHLDDGYPV 227
           F++         +QG    G+  AG+  LA+ LA+++G   IR N IA G +   +G   
Sbjct: 168 FVSATFHYYGVPFQGHV--GAAKAGIDALAKNLAVELGPLGIRSNCIAPGAIDNTEGLKR 227

Query: 228 SVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDGSRYMTGTTIFVDG 273
             GK+  +K + +  PL+R L    D+A + +Y+ S  + Y+TGT + VDG
Sbjct: 228 LAGKKYKEKALAK-IPLQR-LGSTRDIAESTVYIFSPAASYVTGTVLVVDG 266

BLAST of Bhi01G001621 vs. ExPASy Swiss-Prot
Match: Q29529 (Carbonyl reductase [NADPH] 2 OS=Sus scrofa OX=9823 GN=CBR2 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.7e-13
Identity = 54/189 (28.57%), Postives = 101/189 (53.44%), Query Frame = 0

Query: 96  VNRACSILGTLDAFAHAYSYEGPI------------QDALQLSEEEFQKIVRINLMASWF 155
           +   C  LG  +A   A    GP+            Q  L  ++E F +   +NL + + 
Sbjct: 54  IEPVCVDLGDWEATERALGGVGPVDLLVNNAAVALMQPFLDTTKEVFDRSFNVNLRSVFQ 113

Query: 156 LMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDVGKYK 215
           + + V R M ++   GS++ +++++      Y G AAY S    +  L +++A+++G +K
Sbjct: 114 VSQIVARSMIERGVPGSIVNVSSMV--SHVTYPGLAAYSSTKGAMTMLTKSMAMELGPHK 173

Query: 216 IRVNAIARGLHLDD-GYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDGSRY 272
           IRVN++   + L   G  V+   E A+KL KE  P+ ++ +V ED+ +++++L+SD S  
Sbjct: 174 IRVNSVNPTVVLTAMGRSVTSDPELARKL-KERHPMRKFAEV-EDVVNSILFLLSDRSAS 233

BLAST of Bhi01G001621 vs. NCBI nr
Match: XP_038878089.1 (levodione reductase-like [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 2.8e-154
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MTHTQFILYSSTVDYYIIEMERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVL 60
           MTHTQFILYSSTVDYYIIEMERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVL
Sbjct: 1   MTHTQFILYSSTVDYYIIEMERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVL 60

Query: 61  QSMSKVMAESLKGVLPIEVVGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQ 120
           QSMSKVMAESLKGVLPIEVVGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQ
Sbjct: 61  QSMSKVMAESLKGVLPIEVVGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQ 120

Query: 121 DALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAA 180
           DALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAA
Sbjct: 121 DALQLSEEEFQKIVRINLMASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAA 180

Query: 181 YGSCLAGLQQLARTLALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLER 240
           YGSCLAGLQQLARTLALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLER
Sbjct: 181 YGSCLAGLQQLARTLALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLER 240

Query: 241 WLDVKEDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM 286
           WLDVKEDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 WLDVKEDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM 285

BLAST of Bhi01G001621 vs. NCBI nr
Match: NP_001284466.1 (carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydrogenases [Cucumis melo])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 246/266 (92.48%), Postives = 258/266 (96.99%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME ATKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDMEEEREAAFD+AVNRACS+LGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLD+GYPVSVGKERAKKLVK+AA LERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDNGYPVSVGKERAKKLVKDAAKLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. NCBI nr
Match: XP_011651549.1 (uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical protein Csa_017656 [Cucumis sativus])

HSP 1 Score: 481.5 bits (1238), Expect = 5.1e-132
Identity = 244/266 (91.73%), Postives = 257/266 (96.62%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME +TKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VG+DMEEEREAAFD+AVNRAC ILGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K VK+AAPLERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. NCBI nr
Match: KAA0060247.1 (carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cucumis melo var. makuwa])

HSP 1 Score: 470.7 bits (1210), Expect = 8.9e-129
Identity = 239/257 (93.00%), Postives = 250/257 (97.28%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME ATKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDMEEEREAAFD+AVNRACS+LGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVK+AAPLERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSL 277
           SRYMTGTTIFVDGAQSL
Sbjct: 241 SRYMTGTTIFVDGAQSL 257

BLAST of Bhi01G001621 vs. NCBI nr
Match: XP_023529292.1 (carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 464.9 bits (1195), Expect = 4.9e-127
Identity = 231/266 (86.84%), Postives = 252/266 (94.74%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           MERATKNVLLSSNGD+ISKNLAIHLAKRGCRLVL+GNE VLQSM + + ESLKGVLPI V
Sbjct: 1   MERATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLQSMREGIVESLKGVLPIGV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDME EREAAFD+AVN ACS+LGTLDAF HAYSYEGPIQ+ LQLSEEEF+KIV+IN+M
Sbjct: 61  VGLDMEGEREAAFDEAVNGACSVLGTLDAFVHAYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLY G AA+GSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAI+RGLHLDDGYP+SVG ERAKKLVK+AAPLERWLD+K+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAISRGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. ExPASy TrEMBL
Match: Q2PZA4 (Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.9e-133
Identity = 246/266 (92.48%), Postives = 258/266 (96.99%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME ATKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDMEEEREAAFD+AVNRACS+LGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLD+GYPVSVGKERAKKLVK+AA LERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDNGYPVSVGKERAKKLVKDAAKLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. ExPASy TrEMBL
Match: A0A0A0L8L9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.5e-132
Identity = 244/266 (91.73%), Postives = 257/266 (96.62%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME +TKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VG+DMEEEREAAFD+AVNRAC ILGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K VK+AAPLERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. ExPASy TrEMBL
Match: A0A5A7V0A0 (Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G00030 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.3e-129
Identity = 239/257 (93.00%), Postives = 250/257 (97.28%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME ATKNVLLSS GD+ISKNLA+HLA+RGCRLVLIGNE VLQSMSK++AESLKGVLPIEV
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDMEEEREAAFD+AVNRACS+LGTLDAF HAYSYEGPIQDALQLSEEEF+KIV+INLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLY GGAAYGSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVK+AAPLERWLDVK+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSL 277
           SRYMTGTTIFVDGAQSL
Sbjct: 241 SRYMTGTTIFVDGAQSL 257

BLAST of Bhi01G001621 vs. ExPASy TrEMBL
Match: A0A6J1JR50 (carbonyl reductase [NADPH] 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486709 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.7e-125
Identity = 227/266 (85.34%), Postives = 250/266 (93.98%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           MERATKNVLLSSNGD+ISKNLAIHLAKRGCRLVL+GNE VL SM + + ESLKG LPI V
Sbjct: 1   MERATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLHSMREEIGESLKGALPIGV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDME EREAAFD+AV+ ACS+LGTLDAF H+YSYEGPIQ+ LQLSEEEF+KIV+IN+M
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHSYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           +SWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLY G AA+GSC AGLQQLART ALDV
Sbjct: 121 SSWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAIARGLHLDDGYP+SVG ERAKKLVK+AAPLERWLD+K+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

BLAST of Bhi01G001621 vs. ExPASy TrEMBL
Match: A0A6J1EJP8 (carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.0e-125
Identity = 227/266 (85.34%), Postives = 250/266 (93.98%), Query Frame = 0

Query: 20  MERATKNVLLSSNGDDISKNLAIHLAKRGCRLVLIGNEKVLQSMSKVMAESLKGVLPIEV 79
           ME ATKNVLLSSNGD+ISKNLAIHLAKRGCRLVL+GNE VL+SM + + ESLKG LPI V
Sbjct: 1   MEGATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLRSMREGIVESLKGALPIGV 60

Query: 80  VGLDMEEEREAAFDKAVNRACSILGTLDAFAHAYSYEGPIQDALQLSEEEFQKIVRINLM 139
           VGLDME EREAAFD+AV+ ACS+LGTLDAF HAYSYEGPIQ+ LQLSEEEF+KIV+IN+M
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHAYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 140 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYQGGAAYGSCLAGLQQLARTLALDV 199
           ASWFLMKAVCRRMRDQKSGGS++FL++LIG+ERGLY G AA+GSC AGLQQLART ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 200 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVKEAAPLERWLDVKEDLASTVIYLISDG 259
           GKYKIRVNAI+RGLHLDDGYP+SVG ERAKKLVK+AAPLERWLD+K+DLASTVIYLISDG
Sbjct: 181 GKYKIRVNAISRGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 260 SRYMTGTTIFVDGAQSLVRPRMRSYM 286
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G01980.15.9e-8656.02NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G01980.45.9e-8656.02NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G01980.33.3e-8150.34NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G01980.23.0e-4241.35NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G63380.11.9e-3634.11NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q9LBG22.1e-1627.06Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1[more]
P9WGQ25.3e-1527.38Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CD... [more]
P9WGQ35.3e-1527.38Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC... [more]
P325734.5e-1429.87Peroxisomal 2,4-dienoyl-CoA reductase SPS19 [(3E)-enoyl-CoA-producing] OS=Saccha... [more]
Q295291.7e-1328.57Carbonyl reductase [NADPH] 2 OS=Sus scrofa OX=9823 GN=CBR2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038878089.12.8e-154100.00levodione reductase-like [Benincasa hispida][more]
NP_001284466.11.0e-13292.48carbonyl reductase [NADPH] 2 [Cucumis melo] >ABC02082.1 putative alcohol dehydro... [more]
XP_011651549.15.1e-13291.73uncharacterized protein LOC101219109 [Cucumis sativus] >KGN58163.1 hypothetical ... [more]
KAA0060247.18.9e-12993.00carbonyl reductase [Cucumis melo var. makuwa] >TYJ97611.1 carbonyl reductase [Cu... [more]
XP_023529292.14.9e-12786.84carbonyl reductase [NADPH] 2-like [Cucurbita pepo subsp. pepo] >XP_023529293.1 c... [more]
Match NameE-valueIdentityDescription
Q2PZA44.9e-13392.48Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1[more]
A0A0A0L8L92.5e-13291.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1[more]
A0A5A7V0A04.3e-12993.00Carbonyl reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold639G0... [more]
A0A6J1JR501.7e-12585.34carbonyl reductase [NADPH] 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486709 PE... [more]
A0A6J1EJP85.0e-12585.34carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 153..169
score: 21.23
coord: 202..219
score: 31.84
coord: 181..200
score: 26.93
coord: 238..258
score: 23.08
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 36..272
e-value: 2.0E-37
score: 129.0
NoneNo IPR availableGENE3D3.40.50.720coord: 22..282
e-value: 2.5E-39
score: 136.9
NoneNo IPR availablePANTHERPTHR44375BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATEDcoord: 20..285
NoneNo IPR availablePANTHERPTHR44375:SF6F28J7.36 PROTEINcoord: 20..285
NoneNo IPR availableCDDcd05233SDR_ccoord: 40..271
e-value: 7.70682E-41
score: 139.341
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 32..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001621Bhi01M001621mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004090 carbonyl reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity