Bhi01G001533 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001533
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr1: 45421876 .. 45422430 (-)
RNA-Seq ExpressionBhi01G001533
SyntenyBhi01G001533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAACAACGCCGGAATCACCGGACCAGCACTGCCACCGAGCATAGCGGAGCTAGACCTAGCCGAGTTCGACCGAGTAATGAGCGTCAACGTCCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGGGTCATGGTCCCGGCTGGGTCCGGGTCCATCCTATGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCCCACATCCTTATACCATATCGAAACACGCAATTCCGGGGATAGTGAGGTCGGCGGCGACGGAGCTGTGCCGGAACGGGGTAAGAGTGAACTGCATATCGCCGGCGCCTATAGCGACGGCGATGGCGGTGAAGGGGATAGGGGAAATATACAAAGGGGTTAGTAAGGAAGAGATCGTTGGAATAATAAATGGGGTTGGAGTTCTTAAGGGCACAATTTGCGAGGAAGGTGACGTGGCGAAAGCGGCTCTGTTTTTGGCTTCCGATGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGGATTTACTTGCTTTAAAAACCTCGACTTCCCTTCCTTCCACTAG

mRNA sequence

ATGTACAACAACGCCGGAATCACCGGACCAGCACTGCCACCGAGCATAGCGGAGCTAGACCTAGCCGAGTTCGACCGAGTAATGAGCGTCAACGTCCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGGGTCATGGTCCCGGCTGGGTCCGGGTCCATCCTATGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCCCACATCCTTATACCATATCGAAACACGCAATTCCGGGGATAGTGAGGTCGGCGGCGACGGAGCTGTGCCGGAACGGGGTAAGAGTGAACTGCATATCGCCGGCGCCTATAGCGACGGCGATGGCGGTGAAGGGGATAGGGGAAATATACAAAGGGGTTAGTAAGGAAGAGATCGTTGGAATAATAAATGGGGTTGGAGTTCTTAAGGGCACAATTTGCGAGGAAGGTGACGTGGCGAAAGCGGCTCTGTTTTTGGCTTCCGATGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGGATTTACTTGCTTTAAAAACCTCGACTTCCCTTCCTTCCACTAG

Coding sequence (CDS)

ATGTACAACAACGCCGGAATCACCGGACCAGCACTGCCACCGAGCATAGCGGAGCTAGACCTAGCCGAGTTCGACCGAGTAATGAGCGTCAACGTCCGTGGAGTCATAGCCGGAATAAAGCACGCGGCTCGGGTCATGGTCCCGGCTGGGTCCGGGTCCATCCTATGCACCTCAAGCATAAGTGGGCTCATGGGTGGGTTAGGCCCACATCCTTATACCATATCGAAACACGCAATTCCGGGGATAGTGAGGTCGGCGGCGACGGAGCTGTGCCGGAACGGGGTAAGAGTGAACTGCATATCGCCGGCGCCTATAGCGACGGCGATGGCGGTGAAGGGGATAGGGGAAATATACAAAGGGGTTAGTAAGGAAGAGATCGTTGGAATAATAAATGGGGTTGGAGTTCTTAAGGGCACAATTTGCGAGGAAGGTGACGTGGCGAAAGCGGCTCTGTTTTTGGCTTCCGATGATGCCAAATACATCACCGGTCATAATCTCGTCGTCGACGGCGGATTTACTTGCTTTAAAAACCTCGACTTCCCTTCCTTCCACTAG

Protein sequence

MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSISGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKGVSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDFPSFH
Homology
BLAST of Bhi01G001533 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 6.9e-64
Identity = 115/184 (62.50%), Postives = 148/184 (80.43%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           M N+AGI+    PPSIA+LD+  +D+VM +NVRG + GIKHAAR M+PAGSGSILC SSI
Sbjct: 116 MLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTVLGIKHAARAMIPAGSGSILCLSSI 175

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPH Y+ISK  IPG+V++ A+ELC++G+R+NCISPA I T + ++   E + G
Sbjct: 176 SGLMGGLGPHAYSISKFTIPGVVKTVASELCKHGLRINCISPAGIPTPLTLRMFREAFAG 235

Query: 121 --VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNL 180
             + +E+++ I+N  G LKG  CEE DVAKAAL+LASDDAK++TGHNLVVDGGFTCFK+L
Sbjct: 236 HSIREEQLLAIVNATGELKGEKCEEIDVAKAALYLASDDAKFVTGHNLVVDGGFTCFKSL 295

Query: 181 DFPS 183
           + PS
Sbjct: 296 NLPS 299

BLAST of Bhi01G001533 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 6.5e-62
Identity = 110/181 (60.77%), Postives = 141/181 (77.90%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGI GP  P SI++LD+ EF+RVM +NV GV++GIKHAA+ M+PA SG ILCTSS+
Sbjct: 121 MYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVSGIKHAAKFMIPARSGCILCTSSV 180

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           +G+ GGL PH YTISK   PGIV+SAA+ELC +GVR+NCISP  +AT + +  + +++  
Sbjct: 181 AGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRINCISPGTVATPLTLSYLQKVFPK 240

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VS+E++   + G+G LKG  CEE DVAKAAL+LAS+D KY+TGHNLVVDGG T FK   F
Sbjct: 241 VSEEKLRETVKGMGELKGAECEEADVAKAALYLASNDGKYVTGHNLVVDGGMTAFKIAGF 300

Query: 181 P 182
           P
Sbjct: 301 P 301

BLAST of Bhi01G001533 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 4.4e-58
Identity = 106/182 (58.24%), Postives = 136/182 (74.73%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGI     PPSI +LDL  FD+V++ NVRGV+AGIKHAARVM+P  SGSI+C  S+
Sbjct: 158 MYNNAGIPCKT-PPSIVDLDLNVFDKVINTNVRGVMAGIKHAARVMIPRNSGSIICAGSV 217

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           +G+MGGL  H Y++SK A+ GIVRS A+ELC++ +RVNCISP  I T+  +  + +IY G
Sbjct: 218 TGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVNCISPFAITTSFVMDEMRQIYPG 277

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           V    ++ I+   GVL G +CE  DVA AA++LASDD+KY+ GHNLVVDGGFT  K LDF
Sbjct: 278 VDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASDDSKYVNGHNLVVDGGFTTVKTLDF 337

Query: 181 PS 183
           P+
Sbjct: 338 PA 338

BLAST of Bhi01G001533 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-41
Identity = 90/183 (49.18%), Postives = 120/183 (65.57%), Query Frame = 0

Query: 1   MYNNAGITGPALP-PSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAG-SGSILCTS 60
           ++NNAG+ G      SI + D  EFD VM VNVRGV  G+KH AR M+  G  G I+ T+
Sbjct: 117 LFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTA 176

Query: 61  SISGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAV----KGI 120
           S++G+MGG+GPH YT SKHAI G+ ++AA EL + G+RVNCISP  +AT+M V    K  
Sbjct: 177 SVAGVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTS 236

Query: 121 GEIYKGVSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTC 178
           G   +    EE+   +  +  LKG      D+A+AAL+LASD++KY+ GHNLVVDGG T 
Sbjct: 237 GGDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTT 296

BLAST of Bhi01G001533 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-38
Identity = 83/177 (46.89%), Postives = 114/177 (64.41%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           + NNAG+ G A  P I    L+EF+    VNV+G    +KHAARVM+P   GSI+   S+
Sbjct: 103 LINNAGLCG-APCPDIRNYSLSEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSV 162

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
            G++GG+GPH Y  SKHA+ G+ RS A EL ++G+RVNC+SP  +AT +A+  + E  + 
Sbjct: 163 GGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEER- 222

Query: 121 VSKEEIVGIINGVGV---LKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTC 175
            +++  VG  N       LKG      DVA A LFLASDD++YI+G NL++DGGFTC
Sbjct: 223 -TEDAFVGFRNFAAANANLKGVELTVDDVANAVLFLASDDSRYISGDNLMIDGGFTC 276

BLAST of Bhi01G001533 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-40
Identity = 90/183 (49.18%), Postives = 120/183 (65.57%), Query Frame = 0

Query: 1   MYNNAGITGPALP-PSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAG-SGSILCTS 60
           ++NNAG+ G      SI + D  EFD VM VNVRGV  G+KH AR M+  G  G I+ T+
Sbjct: 117 LFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTA 176

Query: 61  SISGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAV----KGI 120
           S++G+MGG+GPH YT SKHAI G+ ++AA EL + G+RVNCISP  +AT+M V    K  
Sbjct: 177 SVAGVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTS 236

Query: 121 GEIYKGVSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTC 178
           G   +    EE+   +  +  LKG      D+A+AAL+LASD++KY+ GHNLVVDGG T 
Sbjct: 237 GGDVEDDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTT 296

BLAST of Bhi01G001533 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-38
Identity = 87/174 (50.00%), Postives = 120/174 (68.97%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           M++NAGI+ P   P I + + A+F+RV SVNV GV   +KHAARVM+PA SG+I+ T+S+
Sbjct: 96  MFSNAGISDPN-RPRIIDNEKADFERVFSVNVTGVFLCMKHAARVMIPARSGNIISTASL 155

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           S  MGG   H Y  SKHA+ G+ R+ A EL + G+RVNC+SP  + TA     +G+ + G
Sbjct: 156 SSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNCLSPFGLPTA-----LGKKFSG 215

Query: 121 V-SKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFT 174
           + ++EE   +IN  G LKG      DVA AAL+LASD+AKY++GHNL +DGGF+
Sbjct: 216 IKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVSGHNLFIDGGFS 263

BLAST of Bhi01G001533 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-38
Identity = 87/198 (43.94%), Postives = 118/198 (59.60%), Query Frame = 0

Query: 3   NNAGITG--PALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 62
           NNAG+ G       SI   D AEFDRV+ VN  G   G+KHAAR M P  +GSI+  +S+
Sbjct: 136 NNAGVLGRQTRAARSILSFDAAEFDRVLRVNALGAALGMKHAARAMAPRRAGSIVSVASV 195

Query: 63  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 122
           + ++GGLGPH YT SKHAI G+ ++AA EL  +GVRVNC+SP  +AT M +    + +  
Sbjct: 196 AAVLGGLGPHAYTASKHAIVGLTKNAACELRAHGVRVNCVSPFGVATPMLINAWRQGHDD 255

Query: 123 VSK--------------------EEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKY 179
            +                     E++  ++ G+  LKG      D+A+A LFLASD+A+Y
Sbjct: 256 ATADADRDLDLDLDVTVPSDQEVEKMEEVVRGLATLKGPTLRPRDIAEAVLFLASDEARY 315

BLAST of Bhi01G001533 vs. ExPASy Swiss-Prot
Match: Q9C826 (Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-37
Identity = 83/177 (46.89%), Postives = 114/177 (64.41%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           + NNAG+ G A  P I    L+EF+    VNV+G    +KHAARVM+P   GSI+   S+
Sbjct: 103 LINNAGLCG-APCPDIRNYSLSEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSV 162

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
            G++GG+GPH Y  SKHA+ G+ RS A EL ++G+RVNC+SP  +AT +A+  + E  + 
Sbjct: 163 GGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEER- 222

Query: 121 VSKEEIVGIINGVGV---LKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTC 175
            +++  VG  N       LKG      DVA A LFLASDD++YI+G NL++DGGFTC
Sbjct: 223 -TEDAFVGFRNFAAANANLKGVELTVDDVANAVLFLASDDSRYISGDNLMIDGGFTC 276

BLAST of Bhi01G001533 vs. ExPASy Swiss-Prot
Match: F4J2Z7 (Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.6e-35
Identity = 84/177 (47.46%), Postives = 117/177 (66.10%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGS-GSILCTSS 60
           +++NAG+       S+ +LDL  FDR M+VNVRG  A IKHAAR MV +G+ GSI+CT+S
Sbjct: 125 LFSNAGVLEAF--GSVLDLDLEAFDRTMAVNVRGAAAFIKHAARSMVASGTRGSIVCTTS 184

Query: 61  ISGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYK 120
           I+  +GG GPH YT SKHA+ G++RSA   L + G+RVN ++P  +AT     G+   Y 
Sbjct: 185 IAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVNGVAPYGVAT-----GMTSAYN 244

Query: 121 GVSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFK 177
             + + +      +G LKG + +   +A+AALFLASDD+ YI+G NLVVDGGF+  K
Sbjct: 245 EEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYISGQNLVVDGGFSVVK 294

BLAST of Bhi01G001533 vs. NCBI nr
Match: KAA0066923.1 (momilactone A synthase-like [Cucumis melo var. makuwa] >TYK30205.1 momilactone A synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 345.9 bits (886), Expect = 2.1e-91
Identity = 171/184 (92.93%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPALPPSIAELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+
Sbjct: 90  MYNNAGITGPALPPSIAELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSM 149

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 150 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRCGVRVNCISPAPVATAMAVKGIGEMYKG 209

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
            SKEEIVGIING+GVLKG ICEE DVAKAALFLASDD+KYITGHNLVVDGGFT FKNLDF
Sbjct: 210 FSKEEIVGIINGLGVLKGAICEEVDVAKAALFLASDDSKYITGHNLVVDGGFTSFKNLDF 269

Query: 181 PSFH 185
           PSFH
Sbjct: 270 PSFH 273

BLAST of Bhi01G001533 vs. NCBI nr
Match: XP_011651586.1 (short-chain dehydrogenase reductase 3c [Cucumis sativus] >KGN58243.1 hypothetical protein Csa_017609 [Cucumis sativus])

HSP 1 Score: 345.5 bits (885), Expect = 2.8e-91
Identity = 169/184 (91.85%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPA+PPSIAELDLA+FDRVM+VNVRGV+AGIKHAARVMVPAG GSILCTSSI
Sbjct: 114 MYNNAGITGPAVPPSIAELDLADFDRVMNVNVRGVVAGIKHAARVMVPAGCGSILCTSSI 173

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR+GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 174 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKG 233

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VSKEEIVGIING+GVLKG ICEE DVAKAALFLA DD+KYITGHNLVVDGGFT FKNLDF
Sbjct: 234 VSKEEIVGIINGLGVLKGAICEEADVAKAALFLACDDSKYITGHNLVVDGGFTSFKNLDF 293

Query: 181 PSFH 185
           PSFH
Sbjct: 294 PSFH 297

BLAST of Bhi01G001533 vs. NCBI nr
Match: XP_008450017.1 (PREDICTED: momilactone A synthase-like [Cucumis melo])

HSP 1 Score: 344.4 bits (882), Expect = 6.2e-91
Identity = 170/184 (92.39%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPALPPSIAELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+
Sbjct: 104 MYNNAGITGPALPPSIAELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSM 163

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR+GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 164 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKG 223

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
            SKEEIVGIING+GVLKG ICEE DVAKAALFLASDD+KYITG NLVVDGGFT FKNLDF
Sbjct: 224 FSKEEIVGIINGLGVLKGAICEEVDVAKAALFLASDDSKYITGQNLVVDGGFTSFKNLDF 283

Query: 181 PSFH 185
           PSFH
Sbjct: 284 PSFH 287

BLAST of Bhi01G001533 vs. NCBI nr
Match: XP_023527081.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.6 bits (880), Expect = 1.1e-90
Identity = 166/184 (90.22%), Postives = 177/184 (96.20%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPALPPSIAELDLAEFDRVM VNVRGV+AGIKHAARVMVP GSGSILCTSSI
Sbjct: 113 MYNNAGITGPALPPSIAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPTGSGSILCTSSI 172

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVR+ ATELCR+GVRVNCISPAP+ATAMAV GIGE+YKG
Sbjct: 173 SGLMGGLGPHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKG 232

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VS+EEI+GIING+GVLKG  CEEGDVAKAAL+LASDDAKYITGHNLVVDGGFTCFKNL+F
Sbjct: 233 VSREEIIGIINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEF 292

Query: 181 PSFH 185
           PS H
Sbjct: 293 PSLH 296

BLAST of Bhi01G001533 vs. NCBI nr
Match: KAG6581134.1 (Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 342.8 bits (878), Expect = 1.8e-90
Identity = 166/184 (90.22%), Postives = 177/184 (96.20%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPA+PPSIAELDLAEFDRVM VNVRGV+AGIKHAARVMVP GSGSILCTSSI
Sbjct: 141 MYNNAGITGPAVPPSIAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPTGSGSILCTSSI 200

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVR+ ATELCR+GVRVNCISPAP+ATAMAV GIGE+YKG
Sbjct: 201 SGLMGGLGPHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKG 260

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VS+EEIVGIING+GVLKG  CEEGDVAKAAL+LASDDAKYITGHNLVVDGGFTCFKNL+F
Sbjct: 261 VSREEIVGIINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEF 320

Query: 181 PSFH 185
           PS H
Sbjct: 321 PSLH 324

BLAST of Bhi01G001533 vs. ExPASy TrEMBL
Match: A0A5D3E4K6 (Momilactone A synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00170 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 1.0e-91
Identity = 171/184 (92.93%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPALPPSIAELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+
Sbjct: 90  MYNNAGITGPALPPSIAELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSM 149

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 150 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRCGVRVNCISPAPVATAMAVKGIGEMYKG 209

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
            SKEEIVGIING+GVLKG ICEE DVAKAALFLASDD+KYITGHNLVVDGGFT FKNLDF
Sbjct: 210 FSKEEIVGIINGLGVLKGAICEEVDVAKAALFLASDDSKYITGHNLVVDGGFTSFKNLDF 269

Query: 181 PSFH 185
           PSFH
Sbjct: 270 PSFH 273

BLAST of Bhi01G001533 vs. ExPASy TrEMBL
Match: A0A0A0L8S5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598890 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.4e-91
Identity = 169/184 (91.85%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPA+PPSIAELDLA+FDRVM+VNVRGV+AGIKHAARVMVPAG GSILCTSSI
Sbjct: 114 MYNNAGITGPAVPPSIAELDLADFDRVMNVNVRGVVAGIKHAARVMVPAGCGSILCTSSI 173

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR+GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 174 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKG 233

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VSKEEIVGIING+GVLKG ICEE DVAKAALFLA DD+KYITGHNLVVDGGFT FKNLDF
Sbjct: 234 VSKEEIVGIINGLGVLKGAICEEADVAKAALFLACDDSKYITGHNLVVDGGFTSFKNLDF 293

Query: 181 PSFH 185
           PSFH
Sbjct: 294 PSFH 297

BLAST of Bhi01G001533 vs. ExPASy TrEMBL
Match: A0A1S3BPB3 (momilactone A synthase-like OS=Cucumis melo OX=3656 GN=LOC103491733 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.0e-91
Identity = 170/184 (92.39%), Postives = 179/184 (97.28%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPALPPSIAELDLA+FDRVMSVNVRGV+AGIKHAARVMVPAGSGSILCTSS+
Sbjct: 104 MYNNAGITGPALPPSIAELDLADFDRVMSVNVRGVVAGIKHAARVMVPAGSGSILCTSSM 163

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVRSAATELCR+GVRVNCISPAP+ATAMAVKGIGE+YKG
Sbjct: 164 SGLMGGLGPHPYSISKHAIPGIVRSAATELCRSGVRVNCISPAPVATAMAVKGIGEMYKG 223

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
            SKEEIVGIING+GVLKG ICEE DVAKAALFLASDD+KYITG NLVVDGGFT FKNLDF
Sbjct: 224 FSKEEIVGIINGLGVLKGAICEEVDVAKAALFLASDDSKYITGQNLVVDGGFTSFKNLDF 283

Query: 181 PSFH 185
           PSFH
Sbjct: 284 PSFH 287

BLAST of Bhi01G001533 vs. ExPASy TrEMBL
Match: A0A6J1J856 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111482148 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 165/184 (89.67%), Postives = 178/184 (96.74%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPA+PPS+AELDLAEFDRVM VNVRGV+AGIKHAARVMVP+GSGSILCTSSI
Sbjct: 113 MYNNAGITGPAVPPSLAELDLAEFDRVMRVNVRGVVAGIKHAARVMVPSGSGSILCTSSI 172

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVR+AATELCR+GVRVNCISPAP+ATAMAV GIGE+YKG
Sbjct: 173 SGLMGGLGPHPYSISKHAIPGIVRAAATELCRSGVRVNCISPAPVATAMAVSGIGEVYKG 232

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VS+EEIVGIING+GVLKG  CEEGDVAKAAL+L SDDAKYITGHNLVVDGGFTCFKNL+F
Sbjct: 233 VSREEIVGIINGLGVLKGAKCEEGDVAKAALYLVSDDAKYITGHNLVVDGGFTCFKNLEF 292

Query: 181 PSFH 185
           PS H
Sbjct: 293 PSLH 296

BLAST of Bhi01G001533 vs. ExPASy TrEMBL
Match: A0A6J1F9M8 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111442080 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.4e-90
Identity = 165/184 (89.67%), Postives = 176/184 (95.65%), Query Frame = 0

Query: 1   MYNNAGITGPALPPSIAELDLAEFDRVMSVNVRGVIAGIKHAARVMVPAGSGSILCTSSI 60
           MYNNAGITGPA+PPSIAELDLAEFDRVM VNVR V+AGIKHAARVMVP GSGSILCTSSI
Sbjct: 113 MYNNAGITGPAVPPSIAELDLAEFDRVMRVNVRAVVAGIKHAARVMVPTGSGSILCTSSI 172

Query: 61  SGLMGGLGPHPYTISKHAIPGIVRSAATELCRNGVRVNCISPAPIATAMAVKGIGEIYKG 120
           SGLMGGLGPHPY+ISKHAIPGIVR+ ATELCR+GVRVNCISPAP+ATAMAV GIGE+YKG
Sbjct: 173 SGLMGGLGPHPYSISKHAIPGIVRAVATELCRSGVRVNCISPAPVATAMAVSGIGEVYKG 232

Query: 121 VSKEEIVGIINGVGVLKGTICEEGDVAKAALFLASDDAKYITGHNLVVDGGFTCFKNLDF 180
           VS+EEIVGIING+GVLKG  CEEGDVAKAAL+LASDDAKYITGHNLVVDGGFTCFKNL+F
Sbjct: 233 VSREEIVGIINGLGVLKGAKCEEGDVAKAALYLASDDAKYITGHNLVVDGGFTCFKNLEF 292

Query: 181 PSFH 185
           PS H
Sbjct: 293 PSLH 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G26760.16.9e-6462.50NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.16.5e-6260.77NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.14.4e-5858.24NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.11.3e-4149.18NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G52340.11.7e-3846.89NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q9SCU01.8e-4049.18Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ... [more]
Q94KL72.2e-3850.00Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
P501602.2e-3843.94Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1[more]
Q9C8262.4e-3746.89Xanthoxin dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=ABA2 PE=1 SV=1[more]
F4J2Z76.6e-3547.46Short-chain dehydrogenase reductase 4 OS=Arabidopsis thaliana OX=3702 GN=SDR4 PE... [more]
Match NameE-valueIdentityDescription
KAA0066923.12.1e-9192.93momilactone A synthase-like [Cucumis melo var. makuwa] >TYK30205.1 momilactone A... [more]
XP_011651586.12.8e-9191.85short-chain dehydrogenase reductase 3c [Cucumis sativus] >KGN58243.1 hypothetica... [more]
XP_008450017.16.2e-9192.39PREDICTED: momilactone A synthase-like [Cucumis melo][more]
XP_023527081.11.1e-9090.22short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo][more]
KAG6581134.11.8e-9090.22Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
A0A5D3E4K61.0e-9192.93Momilactone A synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0L8S51.4e-9191.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598890 PE=4 SV=1[more]
A0A1S3BPB33.0e-9192.39momilactone A synthase-like OS=Cucumis melo OX=3656 GN=LOC103491733 PE=4 SV=1[more]
A0A6J1J8561.1e-9089.67short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1F9M84.4e-9089.67short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 46..62
score: 32.34
coord: 93..110
score: 38.13
coord: 72..91
score: 35.51
coord: 136..156
score: 31.76
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 1..173
e-value: 2.2E-42
score: 145.2
NoneNo IPR availableGENE3D3.40.50.720coord: 1..179
e-value: 3.0E-46
score: 159.9
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 21..174
e-value: 2.3E-14
score: 51.0
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 1..182
NoneNo IPR availablePANTHERPTHR43180:SF55ALCOHOL DEHYDROGENASE-LIKE PROTEINcoord: 1..182
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001533Bhi01M001533mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity