Bhi01G001446 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001446
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)H-quinone oxidoreductase subunit K, chloroplastic
Locationchr1: 41349996 .. 41350268 (+)
RNA-Seq ExpressionBhi01G001446
SyntenyBhi01G001446
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCAGTACCGATTCTTATAGTACTGTTCGGGGAGTCAATAAGTTAATTCCTGTTGATGTGTATTTGCCGGGTTGTCCACCTAAACCCGATGCTATAACAAAACTTCGTAAGAAAATATCTCGAGAAATCTATGAAGATCGAATTAAGTCTCAAGAGGAGAATCGGTGTTTTACGACCAACCACAAATTTCATGTTGGCAATCGACGAGGGATATTCAAAGCATCTAGGAAGAAAGGACGAGCGGTTACTCTAGCAACAGATACGTTCTAA

mRNA sequence

ATGTTCAGTACCGATTCTTATAGTACTGTTCGGGGAGTCAATAAGTTAATTCCTGTTGATGTGTATTTGCCGGGTTGTCCACCTAAACCCGATGCTATAACAAAACTTCGTAAGAAAATATCTCGAGAAATCTATGAAGATCGAATTAAGTCTCAAGAGGAGAATCGGTGTTTTACGACCAACCACAAATTTCATGTTGGCAATCGACGAGGGATATTCAAAGCATCTAGGAAGAAAGGACGAGCGGTTACTCTAGCAACAGATACGTTCTAA

Coding sequence (CDS)

ATGTTCAGTACCGATTCTTATAGTACTGTTCGGGGAGTCAATAAGTTAATTCCTGTTGATGTGTATTTGCCGGGTTGTCCACCTAAACCCGATGCTATAACAAAACTTCGTAAGAAAATATCTCGAGAAATCTATGAAGATCGAATTAAGTCTCAAGAGGAGAATCGGTGTTTTACGACCAACCACAAATTTCATGTTGGCAATCGACGAGGGATATTCAAAGCATCTAGGAAGAAAGGACGAGCGGTTACTCTAGCAACAGATACGTTCTAA

Protein sequence

MFSTDSYSTVRGVNKLIPVDVYLPGCPPKPDAITKLRKKISREIYEDRIKSQEENRCFTTNHKFHVGNRRGIFKASRKKGRAVTLATDTF
Homology
BLAST of Bhi01G001446 vs. TAIR 10
Match: ATCG00430.1 (photosystem II reaction center protein G )

HSP 1 Score: 120.9 bits (302), Expect = 5.1e-28
Identity = 60/78 (76.92%), Postives = 66/78 (84.62%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKI+REIY+DRI+ Q+ NR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKIAREIYKDRIRPQQGNR 173

Query: 61  CFTTNHKF------HVGN 69
           CFTTNHKF      H+GN
Sbjct: 174 CFTTNHKFFVVRSPHIGN 191

BLAST of Bhi01G001446 vs. TAIR 10
Match: AT5G11770.1 (NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial )

HSP 1 Score: 50.1 bits (118), Expect = 1.1e-06
Identity = 21/41 (51.22%), Postives = 32/41 (78.05%), Query Frame = 0

Query: 6   SYSTVRGVNKLIPVDVYLPGCPPKPDA----ITKLRKKISR 43
           SYS VRG ++++PVD+Y+PGCPP  +A    + +L+KKI+R
Sbjct: 168 SYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLLQLQKKINR 208

BLAST of Bhi01G001446 vs. ExPASy Swiss-Prot
Match: Q4VZH1 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus OX=3659 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 3.7e-31
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy Swiss-Prot
Match: B0Z4L3 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera argillicola OX=3940 GN=ndhK PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 8.2e-31
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRI+SQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAIIDAITKLRKKISREIYEDRIRSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy Swiss-Prot
Match: B0Z4U7 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera biennis OX=3942 GN=ndhK PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 8.2e-31
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRI+SQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAIIDAITKLRKKISREIYEDRIRSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy Swiss-Prot
Match: Q9MTP4 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera elata subsp. hookeri OX=85636 GN=ndhK PE=3 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 8.2e-31
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRI+SQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAIIDAITKLRKKISREIYEDRIRSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy Swiss-Prot
Match: B0Z531 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera glazioviana OX=482428 GN=ndhK PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 8.2e-31
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRI+SQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAIIDAITKLRKKISREIYEDRIRSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. NCBI nr
Match: ALO22094.1 (NdhK [Cucurbita ficifolia] >QWV60844.1 NADH-plastoquinone oxidoreductase subunit K [Cucurbita ficifolia] >QZL38790.1 NADH-plastoquinone oxidoreductase subunit K [Cucurbita ficifolia])

HSP 1 Score: 135.6 bits (340), Expect = 2.2e-28
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVGN 69
           CFTTNHKFHVG+
Sbjct: 174 CFTTNHKFHVGS 185

BLAST of Bhi01G001446 vs. NCBI nr
Match: YP_009753878.1 (NADH dehydrogenase subunit K [Trichosanthes lobata] >QIT05826.1 NADH dehydrogenase subunit K [Trichosanthes lobata])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. NCBI nr
Match: YP_009945421.1 (NADH-plastoquinone oxidoreductase subunit K [Sechium edule] >QOE55951.1 NADH-plastoquinone oxidoreductase subunit K [Sechium edule])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. NCBI nr
Match: QIT04137.1 (NADH dehydrogenase subunit K [Sechium edule])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. NCBI nr
Match: YP_009430631.1 (NADH dehydrogenase subunit K [Gynostemma cardiospermum] >YP_009439808.1 NADH dehydrogenase subunit K [Gynostemma laxiflorum] >YP_010015143.1 NADH dehydrogenase subunit K [Gynostemma yixingense] >ART65086.1 NADH dehydrogenase subunit K [Gynostemma cardiospermum] >ATG86666.1 NADH dehydrogenase subunit K [Gynostemma laxiflorum] >QOI14268.1 NADH dehydrogenase subunit K [Gynostemma yixingense])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy TrEMBL
Match: A0A0S2IER2 (NADH-quinone oxidoreductase subunit B OS=Cucurbita ficifolia OX=37645 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-28
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVGN 69
           CFTTNHKFHVG+
Sbjct: 174 CFTTNHKFHVGS 185

BLAST of Bhi01G001446 vs. ExPASy TrEMBL
Match: A0A218KG48 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus var. hardwickii OX=319220 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy TrEMBL
Match: A0A410HF89 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Trichosanthes kirilowii OX=3677 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy TrEMBL
Match: A0A1X9Q1M2 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus OX=3659 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

BLAST of Bhi01G001446 vs. ExPASy TrEMBL
Match: A0A1S4EU13 (NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis melo OX=3656 GN=ndhK PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 66/71 (92.96%), Postives = 67/71 (94.37%), Query Frame = 0

Query: 1   MFSTDSYSTVRGVNKLIPVDVYLPGCPPKP----DAITKLRKKISREIYEDRIKSQEENR 60
           MFSTDSYSTVRGV+KLIPVDVYLPGCPPKP    DAITKLRKKISREIYEDRIKSQEENR
Sbjct: 114 MFSTDSYSTVRGVDKLIPVDVYLPGCPPKPEAVIDAITKLRKKISREIYEDRIKSQEENR 173

Query: 61  CFTTNHKFHVG 68
           CFTTNHKFHVG
Sbjct: 174 CFTTNHKFHVG 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ATCG00430.15.1e-2876.92photosystem II reaction center protein G [more]
AT5G11770.11.1e-0651.22NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial [more]
Match NameE-valueIdentityDescription
Q4VZH13.7e-3192.96NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus OX=36... [more]
B0Z4L38.2e-3191.55NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera argillicola... [more]
B0Z4U78.2e-3191.55NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera biennis OX=... [more]
Q9MTP48.2e-3191.55NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera elata subsp... [more]
B0Z5318.2e-3191.55NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Oenothera glazioviana... [more]
Match NameE-valueIdentityDescription
ALO22094.12.2e-2891.67NdhK [Cucurbita ficifolia] >QWV60844.1 NADH-plastoquinone oxidoreductase subunit... [more]
YP_009753878.12.8e-2892.96NADH dehydrogenase subunit K [Trichosanthes lobata] >QIT05826.1 NADH dehydrogena... [more]
YP_009945421.12.8e-2892.96NADH-plastoquinone oxidoreductase subunit K [Sechium edule] >QOE55951.1 NADH-pla... [more]
QIT04137.12.8e-2892.96NADH dehydrogenase subunit K [Sechium edule][more]
YP_009430631.12.8e-2892.96NADH dehydrogenase subunit K [Gynostemma cardiospermum] >YP_009439808.1 NADH deh... [more]
Match NameE-valueIdentityDescription
A0A0S2IER21.0e-2891.67NADH-quinone oxidoreductase subunit B OS=Cucurbita ficifolia OX=37645 GN=ndhK PE... [more]
A0A218KG481.4e-2892.96NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus var. ... [more]
A0A410HF891.4e-2892.96NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Trichosanthes kirilow... [more]
A0A1X9Q1M21.4e-2892.96NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis sativus OX=36... [more]
A0A1S4EU131.4e-2892.96NAD(P)H-quinone oxidoreductase subunit K, chloroplastic OS=Cucumis melo OX=3656 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.12280coord: 1..63
e-value: 2.7E-14
score: 54.6
NoneNo IPR availablePANTHERPTHR11995NADH DEHYDROGENASEcoord: 1..68
NoneNo IPR availablePANTHERPTHR11995:SF14NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIALcoord: 1..68
NoneNo IPR availableSUPERFAMILY56770HydA/Nqo6-likecoord: 5..48
IPR006137NADH:ubiquinone oxidoreductase-like, 20kDa subunitPFAMPF01058Oxidored_q6coord: 9..35
e-value: 2.5E-6
score: 27.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001446Bhi01M001446mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
biological_process GO:0019684 photosynthesis, light reaction
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0008137 NADH dehydrogenase (ubiquinone) activity
molecular_function GO:0048038 quinone binding
molecular_function GO:0051536 iron-sulfur cluster binding