Bhi01G001334 (gene) Wax gourd (B227) v1

Overview


Name	Bhi01G001334
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr1: 38257673 .. 38260015 (+)
RNA-Seq Expression	Bhi01G001334
Synteny	Bhi01G001334

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCAACCCCTTAAATTTCGTCTCAAACGAAGGACTTTGATAATGAAGAAAAATAAAGTCTTCTTTTCACGGAGTGAAAGACGAGAAGAGAACTGATTCTGCTATTTTTCAAAGTTGAAGGAGAAGAAAAAATGGCGGATTCTCGAGGATCAGACAGCGAAAGTGATAGGCGACGTTCACTTATTAGTAGTAACAGACGCAAGAACGAGGCGATGAACGTCATATTCACTGATCCTCAGATTCTCGATTGCTATATTTGTTGTGAACGTTTGTCAATTCCTGTTTTCCAGGTCCGATTAACCTTTTTTCTGCTTTAAATTCTGTTTATTGTTCATCTGGATGTCTCTGATGCTTTGTTCTTCTCGGCCGTTTTGGGTTTTGTAACTTTGAATACTGTAGTTTTGCTTGTGTGTGTTTGTTGCATTTGACGACTTTGAAATCCCCTTTTTTATTAGGTTTTTATTCTCTCTTTTCTTAAGTTTATTGTGTAGACTGTGGATTGCCATTGGAAGTAATGGATTATTATGGAAAAATTGCAGATAATCCTGGAGTTATAGGACTGCATGATCGAATACTCTTGGAAATTGGGAGAAAATTCTAGATATTATGAGTTCTAATTGCTTATCTTTATCTTGAGTTAATGTCTGAATCAAGCCACAGTTTATTTGGTTTTATCTTCACCTTTTCAATTGTATGGATTATTGGATTTTCTTCACTATTTAGAAACATGAACTAAAAAGGGTATGGATATAGAAGAAAATGAAGTTTACATTTGTGTTTATAATCTCTTATTTTGTTATTTATGAGTCTATTTATTTCATATAAACATGTACTTTGGTGTTGGATGGATTAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAATTCAGAACAAATGCCCTAGCTGCAACTTGGCGATTGGCAACATTCGTTGCAGGGCAATTGAGAAAGTTTTGGAATCAATCAAGATACCGTGCCGAAATGCGATATACGGATGCAAAATGACGATGAGTTTGAATGAGATAGACCACGAGACCTCGTGCACACACGAACCCTGCTTCTGCCCTTTGGACGATTGCACATTTGTTGGTTCAGCTGAGAAGTTGAGCTTACACTTCAGCAAAAAACACAAAAACACATCCAAAAGCTTCTCATACAATGCGAGGTTCACTATGTGCTTGAACAATGGCGATAGTCATCGTATCCTTCAAGCAGAGAACGATAGCATTTTGTTCGTCCTCTCTTACTCATTTGAAATTTTCGGAAATGCAGTAACTGTGAATCGGATTGGACCTTTGTCATCTGAGAGAAAGTTCTGTTACGAAATTAAAGCGAAAACACAGGGGAGTGTTCTTTGTTTCCAATCAATAGCAAAGGAGATCCAAGGTTGGAGTAAAGTTCCTCCTTCCAAAGGATGGCTTTTGATTCCGAACGAGTACTTTGGATCTTCAACCCAGACGATCTTGGAGATTCGCATTTGGCCTGCATGCTAAAGACAAACACTGGGGATCAACACAGGTGAACCTACGTTTGCAAGAAAAGCTGATTATTTATAAACTGAGTTAAGCATTGTTGGCTGGTTGTGATCTTGGTTGCTTGAACTTTGCATCTTTTATGCTTTTTTTGGAAATGACTTGGGTGTCTACTTTTGTACATATTATCATCTTTTGTCTTAGATATCTAGAAAAGGAAAATATTGCATTTTTTTAATTTAGGGAAGATATATGAGTAATTTGATTGATTGGCCTAGAAAATTGGGTCAAAATGTTATTTGACCTCGTTTTATAATCCTTTTCAAACTTTGTTTTAAATATTTCATTTTCTTACCCTCTTCGTTCTATTTCTCTCTCTAAAACTCCCTCCATCATCTCCTTCCTCTAAGATCACTTGATTCCACATCCAATGTAGCAACGATGACAATGAACAAAGTTGTTTCTTCTTTCTTCTACTGTGTGCAGAGTTTTCTCCGACACCAAACAAACTAAGTGTAGAGGCAAGAGTGAAAAAGTGACCAAGAATGAGAGGAAGCGACGAAGTGAGCAAGAGTAAGAAAAGTAAAAAATAGTATGATAAAAGACTTGGTAGTGTCAGCAAAAGGTTGGACATTTTTGTTTTGAAAAAGTCGGTCAAATTTAATTTTTCAGAAATTTTTAGGTCATTCCTACCAAAATGTATCTTTAAGAACGGGAGGGGGCTTTTGCAGGCATAGGTATGTGGGGTTGTGCTTCATATTCTCATCATCCTAGTATAACTTAATCTTATTCTCAGGTTTCACATTCTTTAGTTTTGTAAATTATATCACTTTCTTCTATTTCTGTATATGAAACCACAT

mRNA sequence

AAGCAACCCCTTAAATTTCGTCTCAAACGAAGGACTTTGATAATGAAGAAAAATAAAGTCTTCTTTTCACGGAGTGAAAGACGAGAAGAGAACTGATTCTGCTATTTTTCAAAGTTGAAGGAGAAGAAAAAATGGCGGATTCTCGAGGATCAGACAGCGAAAGTGATAGGCGACGTTCACTTATTAGTAGTAACAGACGCAAGAACGAGGCGATGAACGTCATATTCACTGATCCTCAGATTCTCGATTGCTATATTTGTTGTGAACGTTTGTCAATTCCTGTTTTCCAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAATTCAGAACAAATGCCCTAGCTGCAACTTGGCGATTGGCAACATTCGTTGCAGGGCAATTGAGAAAGTTTTGGAATCAATCAAGATACCGTGCCGAAATGCGATATACGGATGCAAAATGACGATGAGTTTGAATGAGATAGACCACGAGACCTCGTGCACACACGAACCCTGCTTCTGCCCTTTGGACGATTGCACATTTGTTGGTTCAGCTGAGAAGTTGAGCTTACACTTCAGCAAAAAACACAAAAACACATCCAAAAGCTTCTCATACAATGCGAGGTTCACTATGTGCTTGAACAATGGCGATAGTCATCGTATCCTTCAAGCAGAGAACGATAGCATTTTGTTCGTCCTCTCTTACTCATTTGAAATTTTCGGAAATGCAGTAACTGTGAATCGGATTGGACCTTTGTCATCTGAGAGAAAGTTCTGTTACGAAATTAAAGCGAAAACACAGGGGAGTGTTCTTTGTTTCCAATCAATAGCAAAGGAGATCCAAGGTTGGAGTAAAGTTCCTCCTTCCAAAGGATGGCTTTTGATTCCGAACGAGTACTTTGGATCTTCAACCCAGACGATCTTGGAGATTCGCATTTGGCCTGCATGCTAAAGACAAACACTGGGGATCAACACAGAGTTTTCTCCGACACCAAACAAACTAAGTGTAGAGGCAAGAGTGAAAAAGTGACCAAGAATGAGAGGAAGCGACGAAGTGAGCAAGAGTAAGAAAAGTAAAAAATAGTATGATAAAAGACTTGGTAGTGTCAGCAAAAGGTTGGACATTTTTGTTTTGAAAAAGTCGGTCAAATTTAATTTTTCAGAAATTTTTAGGTCATTCCTACCAAAATGTATCTTTAAGAACGGGAGGGGGCTTTTGCAGGCATAGGTATGTGGGGTTGTGCTTCATATTCTCATCATCCTAGTATAACTTAATCTTATTCTCAGGTTTCACATTCTTTAGTTTTGTAAATTATATCACTTTCTTCTATTTCTGTATATGAAACCACAT

Coding sequence (CDS)

ATGGCGGATTCTCGAGGATCAGACAGCGAAAGTGATAGGCGACGTTCACTTATTAGTAGTAACAGACGCAAGAACGAGGCGATGAACGTCATATTCACTGATCCTCAGATTCTCGATTGCTATATTTGTTGTGAACGTTTGTCAATTCCTGTTTTCCAGTGTGAGAATGGGCATATTTCTTGCTCTAGCTGCTGCACCAAAATTCAGAACAAATGCCCTAGCTGCAACTTGGCGATTGGCAACATTCGTTGCAGGGCAATTGAGAAAGTTTTGGAATCAATCAAGATACCGTGCCGAAATGCGATATACGGATGCAAAATGACGATGAGTTTGAATGAGATAGACCACGAGACCTCGTGCACACACGAACCCTGCTTCTGCCCTTTGGACGATTGCACATTTGTTGGTTCAGCTGAGAAGTTGAGCTTACACTTCAGCAAAAAACACAAAAACACATCCAAAAGCTTCTCATACAATGCGAGGTTCACTATGTGCTTGAACAATGGCGATAGTCATCGTATCCTTCAAGCAGAGAACGATAGCATTTTGTTCGTCCTCTCTTACTCATTTGAAATTTTCGGAAATGCAGTAACTGTGAATCGGATTGGACCTTTGTCATCTGAGAGAAAGTTCTGTTACGAAATTAAAGCGAAAACACAGGGGAGTGTTCTTTGTTTCCAATCAATAGCAAAGGAGATCCAAGGTTGGAGTAAAGTTCCTCCTTCCAAAGGATGGCTTTTGATTCCGAACGAGTACTTTGGATCTTCAACCCAGACGATCTTGGAGATTCGCATTTGGCCTGCATGCTAA

Protein sequence

MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHISCSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEIDHETSCTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAENDSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKVPPSKGWLLIPNEYFGSSTQTILEIRIWPAC

Homology

[Close]

BLAST of Bhi01G001334 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 193.4 bits (490), Expect = 2.4e-49
Identity = 105/279 (37.63%), Postives = 155/279 (55.56%), Query Frame = 0


Query: 2   ADSRGSDSESDRRRSLI------------SSNRRKNEAMNVIFTDPQILDCYICCERLSI 61
           ++ RGS S+   R  +I            SS+  K+  ++V   DP +LDC ICCE L I
Sbjct: 63  SEDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKI 122

Query: 62  PVFQCENGHISCSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTM 121
           P+FQC+NGH++C+ CCTK++N+CPSC L IG +RCRA+EKV+E+ ++ C NA YGCK + 
Sbjct: 123 PIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKEST 182

Query: 122 SL-NEIDHETSCTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNN 181
           S  N   HE  C   PC CP+ DC + G  + L+ H   +HK+   SF +N R T+ L+ 
Sbjct: 183 SYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDL 242

Query: 182 GDSHRILQAENDSILFVLSYSFEIFGNAVTVNRIGPLS-SERKFCYEIKAKTQGSVLCFQ 241
                ILQ END  + V+     +    V+V+ I PL+    +    +   T  S+L   
Sbjct: 243 NKKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQG 302

Query: 242 SIAKEIQGWSKVPPSKGWLLIPNEYFGSSTQTILEIRIW 267
            + K IQ  +   P  G++LIP+  F  +    L ++IW
Sbjct: 303 FMVKNIQKVTNEHPEDGFMLIPSYLFSGNDN--LNLQIW 339

[Close]

BLAST of Bhi01G001334 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 165.2 bits (417), Expect = 7.1e-41
Identity = 99/267 (37.08%), Postives = 146/267 (54.68%), Query Frame = 0


Query: 6   GSDSESDRRRSLISSNRRKNEA--MNVIFTDPQILDCYICCERLSIPVFQCENGHISCSS 65
           GS+S   ++R L SS+  K +A   + +  D +ILDC IC E  +IP+FQC+NGH++CSS
Sbjct: 15  GSNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSS 74

Query: 66  CCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSL-NEIDHETSCTH 125
           CC K+ NKCP+C   +G+ RCRA+E VLESI IPC NA  GCK  +S   E+ HE  C  
Sbjct: 75  CCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMF 134

Query: 126 EPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRIL-QAEN-D 185
             C CP  DC +  S + L  H+   H   ++  ++     + +    S +IL + E+  
Sbjct: 135 SHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLT 194

Query: 186 SILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKEIQGWSK 245
           + LF +    E +G  VTV+ I P S E  ++ Y +     G  + +QS   K +   S 
Sbjct: 195 NHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSF 254

Query: 246 VPPSKGWLLIPNEYFGSSTQTILEIRI 266
             P + ++LIPN         +LE+RI
Sbjct: 255 QTPQENFMLIPNSLLRGD---VLEMRI 278

[Close]

BLAST of Bhi01G001334 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 161.0 bits (406), Expect = 1.3e-39
Identity = 94/256 (36.72%), Postives = 140/256 (54.69%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           +++  GS        S     +R ++  + + TD  ILDC IC + L IPVFQC NGH++
Sbjct: 6   LSERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLA 65

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSL-NEIDHETS 120
           CSSCC K++NKCP+C L +G+IRCRA+E+VLES+ +PCR A  GC  T+    E  HE  
Sbjct: 66  CSSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKI 125

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRIL--QA 180
           C   PC CP+  C + GS + L  H+   H   S ++S+N    +      S +IL  + 
Sbjct: 126 CNFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERV 185

Query: 181 ENDSILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKT-QGSVLCFQS-IAKEIQ 240
               +LFV+    E  G  V+V+ I P + E  +F Y +   T +G  + +QS   K++ 
Sbjct: 186 YEKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVL 245

Query: 241 GWSKVPPSKGWLLIPN 251
             S   P   ++LIP+
Sbjct: 246 KVSSQRPKDSFMLIPH 261

[Close]

BLAST of Bhi01G001334 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 149.8 bits (377), Expect = 3.1e-36
Identity = 84/234 (35.90%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 24  KNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHISCSSCCTKIQNKCPSCNLAIGNIR 83
           K +   ++  D  ILDC ICCE L+ P+FQC+NGH++C SCC K+ NKCP+C L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 84  CRAIEKVLESIKIPCRNAIYGC-KMTMSLNEIDHETSCTHEPCFCPLDDCTFVGSAEKLS 143
            RA+E VLESI IPC N  +GC K      E  HE  C    C CP   C + GS + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 144 LHFSKKHKN----TSKSFSYNARFTMCLNNGDSHRILQAENDSILFVLSYSFEIFGNAVT 203
            H+   H        K F     FT  +   D   I +     +L  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 204 VNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKEIQGWSKVPPSKGWLLIPN 251
           V+ I P + E  +F Y++     G  + ++S   K +   S   P + ++LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPH 255

[Close]

BLAST of Bhi01G001334 vs. TAIR 10
Match: AT5G62800.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 145.6 bits (366), Expect = 5.8e-35
Identity = 86/258 (33.33%), Postives = 135/258 (52.33%), Query Frame = 0


Query: 2   ADSRGSDSESDRRRSLISSNRR-KNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 61
           ++S   +  S R+ S     +R +NE  +    D  +LDC +C E L+IP FQC++GHI 
Sbjct: 4   SNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDGHIV 63

Query: 62  CSSCCTKIQNKC--PSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHE 121
           C+ C  K+ NKC  P C+L IGN RC A+E+VLES  +PC+N  +GC  ++S  ++  HE
Sbjct: 64  CNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVSSHE 123

Query: 122 TSCTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFT---MCLNNGDSHRI 181
             C +  C CP  +C + GS   +  HF ++H   S   S    ++   + +N  +   +
Sbjct: 124 KECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYSTVDVLINIKEKVSV 183

Query: 182 LQAENDSILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKE 241
           L      +LFV+    E  G  VTV RI P +SE +KF Y +     G  + ++S   K 
Sbjct: 184 LWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYESPEVKR 243

Query: 242 IQGWSKVPPSKGWLLIPN 251
           +   +   P   ++ +PN
Sbjct: 244 LLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.4e-48
Identity = 105/279 (37.63%), Postives = 155/279 (55.56%), Query Frame = 0


Query: 2   ADSRGSDSESDRRRSLI------------SSNRRKNEAMNVIFTDPQILDCYICCERLSI 61
           ++ RGS S+   R  +I            SS+  K+  ++V   DP +LDC ICCE L I
Sbjct: 63  SEDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKI 122

Query: 62  PVFQCENGHISCSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTM 121
           P+FQC+NGH++C+ CCTK++N+CPSC L IG +RCRA+EKV+E+ ++ C NA YGCK + 
Sbjct: 123 PIFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKEST 182

Query: 122 SL-NEIDHETSCTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNN 181
           S  N   HE  C   PC CP+ DC + G  + L+ H   +HK+   SF +N R T+ L+ 
Sbjct: 183 SYGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDL 242

Query: 182 GDSHRILQAENDSILFVLSYSFEIFGNAVTVNRIGPLS-SERKFCYEIKAKTQGSVLCFQ 241
                ILQ END  + V+     +    V+V+ I PL+    +    +   T  S+L   
Sbjct: 243 NKKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQG 302

Query: 242 SIAKEIQGWSKVPPSKGWLLIPNEYFGSSTQTILEIRIW 267
            + K IQ  +   P  G++LIP+  F  +    L ++IW
Sbjct: 303 FMVKNIQKVTNEHPEDGFMLIPSYLFSGNDN--LNLQIW 339

[Close]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-39
Identity = 99/267 (37.08%), Postives = 146/267 (54.68%), Query Frame = 0


Query: 6   GSDSESDRRRSLISSNRRKNEA--MNVIFTDPQILDCYICCERLSIPVFQCENGHISCSS 65
           GS+S   ++R L SS+  K +A   + +  D +ILDC IC E  +IP+FQC+NGH++CSS
Sbjct: 15  GSNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSS 74

Query: 66  CCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSL-NEIDHETSCTH 125
           CC K+ NKCP+C   +G+ RCRA+E VLESI IPC NA  GCK  +S   E+ HE  C  
Sbjct: 75  CCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMF 134

Query: 126 EPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRIL-QAEN-D 185
             C CP  DC +  S + L  H+   H   ++  ++     + +    S +IL + E+  
Sbjct: 135 SHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLT 194

Query: 186 SILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKEIQGWSK 245
           + LF +    E +G  VTV+ I P S E  ++ Y +     G  + +QS   K +   S 
Sbjct: 195 NHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSF 254

Query: 246 VPPSKGWLLIPNEYFGSSTQTILEIRI 266
             P + ++LIPN         +LE+RI
Sbjct: 255 QTPQENFMLIPNSLLRGD---VLEMRI 278

[Close]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.9e-38
Identity = 94/256 (36.72%), Postives = 140/256 (54.69%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           +++  GS        S     +R ++  + + TD  ILDC IC + L IPVFQC NGH++
Sbjct: 6   LSERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLA 65

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSL-NEIDHETS 120
           CSSCC K++NKCP+C L +G+IRCRA+E+VLES+ +PCR A  GC  T+    E  HE  
Sbjct: 66  CSSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKI 125

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRIL--QA 180
           C   PC CP+  C + GS + L  H+   H   S ++S+N    +      S +IL  + 
Sbjct: 126 CNFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERV 185

Query: 181 ENDSILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKT-QGSVLCFQS-IAKEIQ 240
               +LFV+    E  G  V+V+ I P + E  +F Y +   T +G  + +QS   K++ 
Sbjct: 186 YEKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVL 245

Query: 241 GWSKVPPSKGWLLIPN 251
             S   P   ++LIP+
Sbjct: 246 KVSSQRPKDSFMLIPH 261

[Close]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 4.3e-35
Identity = 84/234 (35.90%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 24  KNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHISCSSCCTKIQNKCPSCNLAIGNIR 83
           K +   ++  D  ILDC ICCE L+ P+FQC+NGH++C SCC K+ NKCP+C L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 84  CRAIEKVLESIKIPCRNAIYGC-KMTMSLNEIDHETSCTHEPCFCPLDDCTFVGSAEKLS 143
            RA+E VLESI IPC N  +GC K      E  HE  C    C CP   C + GS + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 144 LHFSKKHKN----TSKSFSYNARFTMCLNNGDSHRILQAENDSILFVLSYSFEIFGNAVT 203
            H+   H        K F     FT  +   D   I +     +L  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 204 VNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKEIQGWSKVPPSKGWLLIPN 251
           V+ I P + E  +F Y++     G  + ++S   K +   S   P + ++LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYESPEVKRVCKVSIETPQENFMLIPH 255

[Close]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot
Match: Q9FM14 (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 8.2e-34
Identity = 86/258 (33.33%), Postives = 135/258 (52.33%), Query Frame = 0


Query: 2   ADSRGSDSESDRRRSLISSNRR-KNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 61
           ++S   +  S R+ S     +R +NE  +    D  +LDC +C E L+IP FQC++GHI 
Sbjct: 4   SNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDGHIV 63

Query: 62  CSSCCTKIQNKC--PSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHE 121
           C+ C  K+ NKC  P C+L IGN RC A+E+VLES  +PC+N  +GC  ++S  ++  HE
Sbjct: 64  CNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVSSHE 123

Query: 122 TSCTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFT---MCLNNGDSHRI 181
             C +  C CP  +C + GS   +  HF ++H   S   S    ++   + +N  +   +
Sbjct: 124 KECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYSTVDVLINIKEKVSV 183

Query: 182 LQAENDSILFVLSYSFEIFGNAVTVNRIGPLSSE-RKFCYEIKAKTQGSVLCFQS-IAKE 241
           L      +LFV+    E  G  VTV RI P +SE +KF Y +     G  + ++S   K 
Sbjct: 184 LWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYESPEVKR 243

Query: 242 IQGWSKVPPSKGWLLIPN 251
           +   +   P   ++ +PN
Sbjct: 244 LLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of Bhi01G001334 vs. NCBI nr
Match: XP_038893917.1 (E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida])

HSP 1 Score: 561.6 bits (1446), Expect = 3.6e-156
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS
Sbjct: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEIDHETSC 120
           CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEIDHETSC
Sbjct: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEIDHETSC 120

Query: 121 THEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEND 180
           THEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEND
Sbjct: 121 THEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEND 180

Query: 181 SILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKVP 240
           SILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKVP
Sbjct: 181 SILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKVP 240

Query: 241 PSKGWLLIPNEYFGSSTQTILEIRIWPAC 270
           PSKGWLLIPNEYFGSSTQTILEIRIWPAC
Sbjct: 241 PSKGWLLIPNEYFGSSTQTILEIRIWPAC 269

[Close]

BLAST of Bhi01G001334 vs. NCBI nr
Match: XP_004140738.2 (E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypothetical protein Csa_011184 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 6.7e-118
Identity = 211/269 (78.44%), Postives = 233/269 (86.62%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           M DSR SD  S+RR S+I S R  N  +NVIFTDPQILDCYICCE LSIPVFQCENGHI+
Sbjct: 1   MEDSRSSDGGSERRSSVI-SRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIA 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCCTK QNKCPSC LAIG IRCRAIEKVLESIK+PC+NAIYGCK  M LN I DHE+ 
Sbjct: 61  CSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESL 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CPLD+CTFVGS E+L LHF+KKHK+++K FSYN RFT+CLNNGD+HRIL+AEN
Sbjct: 121 CRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D +LF LSY+FEIFGNAVT+NRIGPLSSE+KFCYEIKAKT GSVL  QSIAKEIQG  KV
Sbjct: 181 DGVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKV 240

Query: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 269
           PPSKG LLIPNEYFGSSTQT+LEI IWPA
Sbjct: 241 PPSKGSLLIPNEYFGSSTQTMLEISIWPA 268

[Close]

BLAST of Bhi01G001334 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 424.9 bits (1091), Expect = 5.3e-115
Identity = 204/269 (75.84%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E  +R  +  S R K EAMNVIF+DPQ+LDCYICCE LS PVFQCENGHI+
Sbjct: 1   MADSRASDGERGKRSPV--SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIA 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNE-IDHETS 120
           CSSCCTK+QNKCPSC+L+IG IRCRAIEKVLESIKIPC N+IYGCKMTM+LNE  DH + 
Sbjct: 61  CSSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSL 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           CT+EPC CP+ DC FVGS+EKL  HFSKKHKN++KSFSYN+RFT+CLN+GDS+RILQAE 
Sbjct: 121 CTYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEK 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  LF LS++FEIFGNAVTV+RIGP SSE+KFCYEIKAKTQGS+LC QSIAKEIQG  +V
Sbjct: 181 DGALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV 240

Query: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 269
           PPS   LLIPNE+FGSS QTILE RIWP+
Sbjct: 241 PPSNRSLLIPNEFFGSSAQTILEFRIWPS 267

[Close]

BLAST of Bhi01G001334 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 411.4 bits (1056), Expect = 6.1e-111
Identity = 196/270 (72.59%), Postives = 235/270 (87.04%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E +RRRS ++ +R KNEAM VIFTDPQILDCYICCE LS+PV+QCENGHIS
Sbjct: 1   MADSRSSDGEKERRRS-VNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCC+K+ NKCPSC+LAIG IRCRAIEKVLESIKIPC+N+ YGCK+TM+LN+I +HETS
Sbjct: 61  CSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETS 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CP+ DC++V SAE L  HF K+H++++KSF YN+RFT+CLN+GD +RI QAEN
Sbjct: 121 CPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  LFVLSYSFEIFGNAVT++RIG   SE+KFCY+IKAK+QGSVL  QS+AKEIQ W++ 
Sbjct: 181 DDALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTED 240

Query: 241 PPSKGWLLIPNEYFGSST-QTILEIRIWPA 269
           PP KG LLIPNE+FGSS+ QTILEIRIWP+
Sbjct: 241 PPFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Bhi01G001334 vs. NCBI nr
Match: KAG6606996.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405.6 bits (1041), Expect = 3.3e-109
Identity = 193/270 (71.48%), Postives = 232/270 (85.93%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E +RRRS ++ +R KNEAM VIFTDPQILDCYICCE LS+PV+QCENGHIS
Sbjct: 1   MADSRSSDGEKERRRS-VNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCC+K+ NKCPSC+LAIG IRCRAIEKVLESIKIPC+N+ YGCK+TM+LN+I +HETS
Sbjct: 61  CSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETS 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CP+ DC++V SAE L  HF K+H++++KSF YN+RFT+CLN+GD +RI QAEN
Sbjct: 121 CPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  LFVLSYSFEIFGNAVT++RIG    E+KFCY+IKAK+QGSVL  QS+A EI+ W + 
Sbjct: 181 DDALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIED 240

Query: 241 PPSKGWLLIPNEYFGSST-QTILEIRIWPA 269
           PP KG LLIPNE+FGSS+ QTILEIRIWP+
Sbjct: 241 PPFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL
Match: A0A0A0L9Z6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.2e-118
Identity = 211/269 (78.44%), Postives = 233/269 (86.62%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           M DSR SD  S+RR S+I S R  N  +NVIFTDPQILDCYICCE LSIPVFQCENGHI+
Sbjct: 1   MEDSRSSDGGSERRSSVI-SRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIA 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCCTK QNKCPSC LAIG IRCRAIEKVLESIK+PC+NAIYGCK  M LN I DHE+ 
Sbjct: 61  CSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESL 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CPLD+CTFVGS E+L LHF+KKHK+++K FSYN RFT+CLNNGD+HRIL+AEN
Sbjct: 121 CRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D +LF LSY+FEIFGNAVT+NRIGPLSSE+KFCYEIKAKT GSVL  QSIAKEIQG  KV
Sbjct: 181 DGVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKV 240

Query: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 269
           PPSKG LLIPNEYFGSSTQT+LEI IWPA
Sbjct: 241 PPSKGSLLIPNEYFGSSTQTMLEISIWPA 268

[Close]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.6e-115
Identity = 204/269 (75.84%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E  +R  +  S R K EAMNVIF+DPQ+LDCYICCE LS PVFQCENGHI+
Sbjct: 1   MADSRASDGERGKRSPV--SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIA 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNE-IDHETS 120
           CSSCCTK+QNKCPSC+L+IG IRCRAIEKVLESIKIPC N+IYGCKMTM+LNE  DH + 
Sbjct: 61  CSSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSL 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           CT+EPC CP+ DC FVGS+EKL  HFSKKHKN++KSFSYN+RFT+CLN+GDS+RILQAE 
Sbjct: 121 CTYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEK 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  LF LS++FEIFGNAVTV+RIGP SSE+KFCYEIKAKTQGS+LC QSIAKEIQG  +V
Sbjct: 181 DGALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEV 240

Query: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 269
           PPS   LLIPNE+FGSS QTILE RIWP+
Sbjct: 241 PPSNRSLLIPNEFFGSSAQTILEFRIWPS 267

[Close]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.9e-111
Identity = 196/270 (72.59%), Postives = 235/270 (87.04%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E +RRRS ++ +R KNEAM VIFTDPQILDCYICCE LS+PV+QCENGHIS
Sbjct: 1   MADSRSSDGEKERRRS-VNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCC+K+ NKCPSC+LAIG IRCRAIEKVLESIKIPC+N+ YGCK+TM+LN+I +HETS
Sbjct: 61  CSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETS 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CP+ DC++V SAE L  HF K+H++++KSF YN+RFT+CLN+GD +RI QAEN
Sbjct: 121 CPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  LFVLSYSFEIFGNAVT++RIG   SE+KFCY+IKAK+QGSVL  QS+AKEIQ W++ 
Sbjct: 181 DDALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTED 240

Query: 241 PPSKGWLLIPNEYFGSST-QTILEIRIWPA 269
           PP KG LLIPNE+FGSS+ QTILEIRIWP+
Sbjct: 241 PPFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 6.1e-109
Identity = 192/270 (71.11%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MADSR SD E +RRRS ++ +R KNEAM VIFTDPQILDCYICCE LS+PV+QCENGHIS
Sbjct: 1   MADSRSSDGEKERRRS-VNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCC+K+ NKCPSC+LAIG IRCRAIEKVLESIKIPC+N+ YGCK+TM+LN+I +HETS
Sbjct: 61  CSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETS 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C +EPC CP+ DC++V SAE L  HF K+H +++KSF YN+RFT+CLN+GD +RI QAEN
Sbjct: 121 CPYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D  +FVLSYSFEIFGNAVT++RIG    E+KFCY+IKAK+QGSVL  QS+A EI+ W + 
Sbjct: 181 DDAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIED 240

Query: 241 PPSKGWLLIPNEYFGSST-QTILEIRIWPA 269
           PP KG LLIPNE+FGSS+ QTILEIRIWP+
Sbjct: 241 PPFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.3e-103
Identity = 182/270 (67.41%), Postives = 225/270 (83.33%), Query Frame = 0


Query: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60
           MA SR SD E +RR S ++S+R KNE M VI TDPQ+LDCYICC  LS+PV+QCENGHIS
Sbjct: 1   MAGSRSSDGEKERRSS-VNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHIS 60

Query: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120
           CSSCC+K+ NKCPSC+L IG +RCRAIEKVLESIK+PC+N+ YGCK T +LN+I +HET 
Sbjct: 61  CSSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETL 120

Query: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180
           C ++PC CPL DC++V SAE L  HF K+H++++KS SYN+RFT+CLN+ D + I QAEN
Sbjct: 121 CPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEN 180

Query: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240
           D +LFVLSYSFE FG+AVT+ RIGP SSE+KFCY+IKAK+ G+VL   S+AKEIQ W++ 
Sbjct: 181 DGVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTED 240

Query: 241 PPSKGWLLIPNEYFGSST-QTILEIRIWPA 269
           PPSKG LLIPNE+FGSS+ QTILEIRIWP+
Sbjct: 241 PPSKGSLLIPNEFFGSSSAQTILEIRIWPS 269

The following BLAST results are available for this feature:

BLAST of Bhi01G001334 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37930.1	2.4e-49	37.63	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	7.1e-41	37.08	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	1.3e-39	36.72	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37910.1	3.1e-36	35.90	Protein with RING/U-box and TRAF-like domains	[more]
AT5G62800.1	5.8e-35	33.33	Protein with RING/U-box and TRAF-like domains	[more]

BLAST of Bhi01G001334 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K34	3.4e-48	37.63	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD7	1.0e-39	37.08	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]
Q9FKD9	1.9e-38	36.72	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FKD5	4.3e-35	35.90	Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FM14	8.2e-34	33.33	E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]

BLAST of Bhi01G001334 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893917.1	3.6e-156	100.00	E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ...	[more]
XP_004140738.2	6.7e-118	78.44	E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypotheti...	[more]
XP_022143325.1	5.3e-115	75.84	E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia]	[more]
XP_022997791.1	6.1e-111	72.59	E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima]	[more]
KAG6606996.1	3.3e-109	71.48	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Bhi01G001334 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9Z6	3.2e-118	78.44	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE...	[more]
A0A6J1CNI1	2.6e-115	75.84	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1K8G5	2.9e-111	72.59	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P...	[more]
A0A6J1GBP1	6.1e-109	71.11	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508...	[more]
A0A6J1KAW2	2.3e-103	67.41	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 82..234 e-value: 3.8E-17 score: 62.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 88..145 e-value: 1.4E-14 score: 56.0
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 10..267
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 93..254
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 10..267
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 40..76 score: 8.668251
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 93..150 score: 13.323719

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi01M001334	Bhi01M001334	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding