Bhi01G001266 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001266
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDDE Tnp4 domain-containing protein
Locationchr1: 35298746 .. 35301896 (+)
RNA-Seq ExpressionBhi01G001266
SyntenyBhi01G001266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTAACTCGTATACTCACATTGAGAGAGGAAAAAAAAAAAAAAAAAACTCATAAACCCTAACTCCAAAGGAAACGTAAGAGAAAGATACCAAATAAAGGAGCAAGTTGACAGGCACATGAAACCAAAAAGATCGCGGAGATTGGCGAGCTGGTCTGACCAGGCAGGTGTTGGTATGCCGTAATTTCTACAAGATTCAACATCTCCCAGGTCCGGCGCTGACAAAAAAAAAACTGAAAACCCATCTCCTTCGCAGCCGCTCGATTCCCAAGAGAATCGAAAGTCCCAATTCTCAACTACTGCAACTGCAAAACTGACACAAACCCATTAATGGATTCCCCTCAATTGGCTGCTTTACTCTCTTCTTTGATCTCTCAACTCCTCCTCCTTCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCACTCCCGATTCCAATTTCTATGCCAATCTATTCACCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCCCTCCGACCACCTCGAATTGGGCTCATCCCATGGTCGATTTGATCATTTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACCTCCTCAACGTTCGAATGGCTCTCTGGTTTGCTTGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCGCCTCTTGATCTCTCCGTTGAGATTCGACTCGGTGTTGGCCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCAAAATCTCCCACCAATTTGGCGTCTCGGAATCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGTGGCGTGGTTACTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGTGATAAGGACGATTCCACGGTGCTTATGTCTTCGACGCTGTTTAAAGACATCGAACAAGGAAGGCTTCTGGATGCTCCTCCAGTTTACCTTCATGGGGTGGCTGTCAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATGCTGCCTTTTGCAGGTGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATAAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCAAACCAATGCATGAGGAGTTCAAAACTGCAGTTGCTTATATTGGTGCATGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGACTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAGATCTCAGTATGTTGAAGATAAATTGAATGAGGATTCAACTAATGAGAAGGCTTCTATTATACAGAGGGCGCTGGCTGTGAGAGCTAGAGAGCTTCACAGTTAAAATTTCAATCACAAGAATGCAGTTGTTTGCTGAAATAAGTACAGCTCAATTGAAGGAGATTTCCATCTCTTAGGATATTTATTGAAGGCAGCTCATCCAGCTCCATTCAACAATTACTATTGTAGGTAATTGATGATTCTTTTACAAATTTATATAACATTTTCTCCCAAATTTTGGATGTTCAGCTCTTGATTTATCATTTCTTTTCCTTTTTTTCGCAAAACTGGTCCCTATTTTTTAGTTCTATGTCTAGTATTAGGTTAATGATTTTAAGATTATCAAGAAAGTAGGCAAAAGAATGGGATATTTGGAGATACCTTTAAGGCCAGGGCATTCTTCAAGTGGGAAAGTTGATAATATAGGACCCTTTTTTGGGGCAATCTCCCTAATATTGATGGAGTGACTAATTTATTGGATCCTTAATGTAGATTATTCATTCTTTTTATTGTTTTCACACCACAGAATTGCCTACTTTTCAGTCATCTTGCTGTTAAAAGCTCTATGAAAGCTTAAATTTCATTGCTGCCTGCCCCATGGTTAATTTGGCAGACACCACACACCACATCCTGCTGTGGGTTAAAGTTAGACTAGTTTCTTTTTTCACCATTTGAGATCATTGAATTCTCAACTCTTAACTATCACTGTGTCTTCTGGTTGGACTATGAAGGTAAACATTTCCGGCCTATTCGGAATGACTTTCCAAGTGCTCAAAAAAAGATGTTAAAATGCAGCAAAATCGTTTGTAAGCACATG

mRNA sequence

TGTAACTCGTATACTCACATTGAGAGAGGAAAAAAAAAAAAAAAAAACTCATAAACCCTAACTCCAAAGGAAACGTAAGAGAAAGATACCAAATAAAGGAGCAAGTTGACAGGCACATGAAACCAAAAAGATCGCGGAGATTGGCGAGCTGGTCTGACCAGGCAGGTGTTGGTATGCCGTAATTTCTACAAGATTCAACATCTCCCAGGTCCGGCGCTGACAAAAAAAAAACTGAAAACCCATCTCCTTCGCAGCCGCTCGATTCCCAAGAGAATCGAAAGTCCCAATTCTCAACTACTGCAACTGCAAAACTGACACAAACCCATTAATGGATTCCCCTCAATTGGCTGCTTTACTCTCTTCTTTGATCTCTCAACTCCTCCTCCTTCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCACTCCCGATTCCAATTTCTATGCCAATCTATTCACCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCCCTCCGACCACCTCGAATTGGGCTCATCCCATGGTCGATTTGATCATTTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACCTCCTCAACGTTCGAATGGCTCTCTGGTTTGCTTGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCGCCTCTTGATCTCTCCGTTGAGATTCGACTCGGTGTTGGCCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCAAAATCTCCCACCAATTTGGCGTCTCGGAATCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGTGGCGTGGTTACTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGTGATAAGGACGATTCCACGGTGCTTATGTCTTCGACGCTGTTTAAAGACATCGAACAAGGAAGGCTTCTGGATGCTCCTCCAGTTTACCTTCATGGGGTGGCTGTCAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATGCTGCCTTTTGCAGGTGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATAAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCAAACCAATGCATGAGGAGTTCAAAACTGCAGTTGCTTATATTGGTGCATGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGACTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAGATCTCAGTATGTTGAAGATAAATTGAATGAGGATTCAACTAATGAGAAGGCTTCTATTATACAGAGGGCGCTGGCTGTGAGAGCTAGAGAGCTTCACAGTTAAAATTTCAATCACAAGAATGCAGTTGTTTGCTGAAATAAGTACAGCTCAATTGAAGGAGATTTCCATCTCTTAGGATATTTATTGAAGGCAGCTCATCCAGCTCCATTCAACAATTACTATTAATTGCCTACTTTTCAGTCATCTTGCTGTTAAAAGCTCTATGAAAGCTTAAATTTCATTGCTGCCTGCCCCATGGTTAATTTGGCAGACACCACACACCACATCCTGCTGTGGGTTAAAGTTAGACTAGTTTCTTTTTTCACCATTTGAGATCATTGAATTCTCAACTCTTAACTATCACTGTGTCTTCTGGTTGGACTATGAAGGTAAACATTTCCGGCCTATTCGGAATGACTTTCCAAGTGCTCAAAAAAAGATGTTAAAATGCAGCAAAATCGTTTGTAAGCACATG

Coding sequence (CDS)

ATGGATTCCCCTCAATTGGCTGCTTTACTCTCTTCTTTGATCTCTCAACTCCTCCTCCTTCTCTTCCTCCTCTTCCCTTCCTCCAACCCACATTCCCTTTTCTCCAATTCCACTCCCGATTCCAATTTCTATGCCAATCTATTCACCCACTTCCTCTTTTCCCAGGATTTTGCCGCTTCCCTTCCCTTTCTCTCTGTTTCCCGCAAGAGGAAGAGAACCAATCCCTCCGACCACCTCGAATTGGGCTCATCCCATGGTCGATTTGATCATTTGTTTCGGACTCGGACTCCTGATTCTTTCAGAAATCACTTCAGAATGACCTCCTCAACGTTCGAATGGCTCTCTGGTTTGCTTGAGCCCCTTCTCGAGTGTCGTGACCCGGTGGGTTCGCCTCTTGATCTCTCCGTTGAGATTCGACTCGGTGTTGGCCTGTATCGCCTCGCCACCGGCTGCGATTTCTCCAAAATCTCCCACCAATTTGGCGTCTCGGAATCGGTAGCGAGGTTCTGTGCTAAACAATTGTGTCGAGTTCTCTGTACTAATTTTCGCTTCTGGGTTGAATTCCCTTGCCCCAATGAGCTCGAATTAACGTCCTCGGCTTTTGAAGATCTTGCTGGGCTTCCGAATTGCTGTGGCGTGGTTACTTGTACAAGGTTCAAGATCATTAGAAATAGCCATTTTTATGAAGATAGCATCGCTACTCAACTTGTTGTTGATTCCTCGTCACGAATCCTTAGTATTGTTGCAGGATTTCGTGGTGATAAGGACGATTCCACGGTGCTTATGTCTTCGACGCTGTTTAAAGACATCGAACAAGGAAGGCTTCTGGATGCTCCTCCAGTTTACCTTCATGGGGTGGCTGTCAATCAGTACTTGTTTGGACATGGTGAATACCCTTTGCTTCCATGGTTAATGCTGCCTTTTGCAGGTGCTGTTTCAGGGTCAACTGAAGAGAGTTTCAATAAAGCTCACCGATTGATGTGCATTCCAGCTCTGAAAGCAATTGTTAGTTTGAGAAATTGGGGAGTTTTGAGCAAACCAATGCATGAGGAGTTCAAAACTGCAGTTGCTTATATTGGTGCATGCTCAATTCTTCATAATGCTTTGTTGATGAGGGAGGACTTTTCTGCCATGGCTGATGAGTGGGAGAGCTTAGCTTCATTTGATCATAGATCTCAGTATGTTGAAGATAAATTGAATGAGGATTCAACTAATGAGAAGGCTTCTATTATACAGAGGGCGCTGGCTGTGAGAGCTAGAGAGCTTCACAGTTAA

Protein sequence

MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAASLPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRARELHS
Homology
BLAST of Bhi01G001266 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-29
Identity = 87/303 (28.71%), Postives = 145/303 (47.85%), Query Frame = 0

Query: 97  PDSFRNHFRMTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGC 156
           P +F + F+++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G 
Sbjct: 69  PKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGE 128

Query: 157 DFSKISHQFGVSESVA-----RFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAG 216
             S I   FG+++S       RF      R +  +   W     P++L+   S FE ++G
Sbjct: 129 SLSVIGETFGMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISG 188

Query: 217 LPNCCGVVTCTRFKIIRNSHFYED------------SIATQLVVDSSSRILSIVAGFRGD 276
           LPNCCG +  T   I+ N    E             S+  Q VVD   R L ++AG+ G 
Sbjct: 189 LPNCCGAIDITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGS 248

Query: 277 KDDSTVLMSSTLFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLMLPFAGAVS 336
            +D  VL +S  +K +E+G+ L+   + L     + +Y+ G   +PLLPWL+ P+ G  +
Sbjct: 249 LNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPT 308

Query: 337 GSTEESFNKAHRLMCIPALKAIVSLRN-WGVLSKPMHEEFKTAV-AYIGACSILHNALLM 375
              +  FNK H      A  A+  L++ W +++  M    +  +   I  C +LHN ++ 
Sbjct: 309 SLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIID 360

BLAST of Bhi01G001266 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-28
Identity = 86/293 (29.35%), Postives = 137/293 (46.76%), Query Frame = 0

Query: 99  SFRNHFRMTSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFS 158
           +F++ FR + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    
Sbjct: 64  AFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQV 123

Query: 159 KISHQFGVSESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV 218
            +   FGV +S       +    L    +  + +P  + +E   S FE++ GLPNCCG +
Sbjct: 124 SVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAI 183

Query: 219 TCTR----FKIIRNSHFYED-----SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSST 278
             T        ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S 
Sbjct: 184 DTTHIIMTLPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSG 243

Query: 279 LFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAH 338
            FK  E  ++LD  P  L  G  + +Y+ G   YPLLPWL+ P        +  +FN+ H
Sbjct: 244 FFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERH 303

Query: 339 RLMCIPALKAIVSLR-NWGVLSKPM-HEEFKTAVAYIGACSILHNALLMREDF 376
             +   A  A   L+ +W +LSK M   + +   + I  C +LHN ++   D+
Sbjct: 304 EKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Bhi01G001266 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 95.9 bits (237), Expect = 8.3e-20
Identity = 77/297 (25.93%), Postives = 124/297 (41.75%), Query Frame = 0

Query: 98  DSFRNHFRMTSSTFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKIS 157
           + F+  FRM+ STFE +   L   +  ++       + V  R+ V ++RLATG     +S
Sbjct: 173 EDFKKAFRMSKSTFELICDELNSAV-AKEDTALRNAIPVRQRVAVCIWRLATGEPLRLVS 232

Query: 158 HQFGVSESVARFCAKQLCR----VLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGV 217
            +FG+  S       ++C+    VL   +  W   P    L      FE ++G+PN  G 
Sbjct: 233 KKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW---PDDESLRNIRERFESVSGIPNVVGS 292

Query: 218 VTCTRFKIIR-----NSHFYED----------SIATQLVVDSSSRILSIVAGFRGDKDDS 277
           +  T   II       S+F +           SI  Q VV+       +  G+ G   D 
Sbjct: 293 MYTTHIPIIAPKISVASYFNKRHTERNQKTSYSITIQAVVNPKGVFTDLCIGWPGSMPDD 352

Query: 278 TVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEE 337
            VL  S L++    G LL              ++ G   +PLL W+++P+       T+ 
Sbjct: 353 KVLEKSLLYQRANNGGLLK-----------GMWVAGGPGHPLLDWVLVPYTQQNLTWTQH 412

Query: 338 SFNKAHRLMCIPALKAIVSLR-NWGVLSKPMHEEFKTAVAYIGACSILHNALLMRED 375
           +FN+    +   A +A   L+  W  L K    + +     +GAC +LHN   MRE+
Sbjct: 413 AFNEKMSEVQGVAKEAFGRLKGRWACLQKRTEVKLQDLPTVLGACCVLHNICEMREE 454

BLAST of Bhi01G001266 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 95.5 bits (236), Expect = 1.1e-19
Identity = 82/314 (26.11%), Postives = 133/314 (42.36%), Query Frame = 0

Query: 88  FDHLFRTRTP-DSFRNHFRMTSSTFEWLSGLLEPLLE-----CRDPVGSPLDLSVEIRLG 147
           +D + R   P D FR  FRM+ STF  +   L+  +       RD + +P       R+G
Sbjct: 200 WDRVSRPDFPEDEFRREFRMSKSTFNLICEELDTTVTKKNTMLRDAIPAPK------RVG 259

Query: 148 VGLYRLATGCDFSKISHQFGVSESVARFCAKQLCR----VLCTNFRFWVEFPCPNELELT 207
           V ++RLATG     +S +FG+  S       ++CR    VL   +  W   P  +E+  T
Sbjct: 260 VCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW---PSDSEINST 319

Query: 208 SSAFEDLAGLPNCCGVVTCTRFKIIR---------NSHFYED------SIATQLVVDSSS 267
            + FE +  +PN  G +  T   II          N    E       SI  Q VV++  
Sbjct: 320 KAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSITVQGVVNADG 379

Query: 268 RILSIVAGFRGDKDDSTVLMSSTLFKD-IEQGRLLDAPPVYLHGVAVNQYLFGHGEYPLL 327
               +  G  G   D  +L  S+L +    +G L D+            ++ G+  +PL 
Sbjct: 380 IFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDS------------WIVGNSGFPLT 439

Query: 328 PWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLR-NWGVLSKPMHEEFKTAVAYIG 375
            +L++P+       T+ +FN++   +   A  A   L+  W  L K    + +     +G
Sbjct: 440 DYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQKRTEVKLQDLPYVLG 492

BLAST of Bhi01G001266 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 86.7 bits (213), Expect = 5.0e-17
Identity = 92/392 (23.47%), Postives = 163/392 (41.58%), Query Frame = 0

Query: 12  SLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHF----LFSQDFAASLPFLSVS 71
           +++S LL L   L P+S   S  S S+  S   ++L +      L     A+ L FL+V+
Sbjct: 7   AMLSHLLHLQNSLDPTSTLFSSASTSSQSSTTPSSLLSTSSAAPLLFFTLASLLSFLAVN 66

Query: 72  RKRKRTNPSDHLELGS-----SHGRF----------DHLFRTRTP---DSFRNHFRMTSS 131
           R    ++ S      S     + G +          DH++    P     +R+ + ++  
Sbjct: 67  RSSTESSSSSESPSPSPPPPLADGDYSVAAFRALTTDHIWSLDAPLRDARWRSLYGLSYP 126

Query: 132 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARF 191
            F  +   L+P +       S L L  +  + + L RLA GC    ++ ++ +   +   
Sbjct: 127 VFITVVDKLKPFI-----TASNLSLPADYAVAMVLSRLAHGCSAKTLASRYSLDPYLISK 186

Query: 192 CAKQLCRVLCTN-FRFWVEFPC-PNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNS-- 251
               + R+L T  +  +++ P     L  T+  FE+L  LPN CG +  T  K+ R +  
Sbjct: 187 ITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEELTSLPNICGAIDSTPVKLRRRTKL 246

Query: 252 --------HFYEDSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLD 311
                    +  D++  Q+V D       +     G +DDS+    S L+K +  G ++ 
Sbjct: 247 NPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRLTSGDIVW 306

Query: 312 APPVYLHGVAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVS 368
              + + G  V  Y+ G   YPLL +LM PF+   SG+  E+      +     +   + 
Sbjct: 307 EKVINIRGHHVRPYIVGDWCYPLLSFLMTPFSPNGSGTPPENLFDGMLMKGRSVVVEAIG 366

BLAST of Bhi01G001266 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.8e-28
Identity = 87/303 (28.71%), Postives = 145/303 (47.85%), Query Frame = 0

Query: 97  PDSFRNHFRMTSSTFEWLSGLLEPLLECR-----DPVGSPLDLSVEIRLGVGLYRLATGC 156
           P +F + F+++  TF+++  L++     +     D  G+P  LS+  R+ V L RL +G 
Sbjct: 69  PKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNP--LSLNDRVAVALRRLGSGE 128

Query: 157 DFSKISHQFGVSESVA-----RFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAG 216
             S I   FG+++S       RF      R +  +   W     P++L+   S FE ++G
Sbjct: 129 SLSVIGETFGMNQSTVSQITWRFVESMEERAI--HHLSW-----PSKLDEIKSKFEKISG 188

Query: 217 LPNCCGVVTCTRFKIIRNSHFYED------------SIATQLVVDSSSRILSIVAGFRGD 276
           LPNCCG +  T   I+ N    E             S+  Q VVD   R L ++AG+ G 
Sbjct: 189 LPNCCGAIDITH--IVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWPGS 248

Query: 277 KDDSTVLMSSTLFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLMLPFAGAVS 336
            +D  VL +S  +K +E+G+ L+   + L     + +Y+ G   +PLLPWL+ P+ G  +
Sbjct: 249 LNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGKPT 308

Query: 337 GSTEESFNKAHRLMCIPALKAIVSLRN-WGVLSKPMHEEFKTAV-AYIGACSILHNALLM 375
              +  FNK H      A  A+  L++ W +++  M    +  +   I  C +LHN ++ 
Sbjct: 309 SLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNIIID 360

BLAST of Bhi01G001266 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.8e-27
Identity = 86/293 (29.35%), Postives = 137/293 (46.76%), Query Frame = 0

Query: 99  SFRNHFRMTSSTFEWLSGLLEPLLECRDPVG----SPLDLSVEIRLGVGLYRLATGCDFS 158
           +F++ FR + +TF ++  L+   L  R P G        LSVE ++ + L RLA+G    
Sbjct: 64  AFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQV 123

Query: 159 KISHQFGVSESVARFCAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV 218
            +   FGV +S       +    L    +  + +P  + +E   S FE++ GLPNCCG +
Sbjct: 124 SVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSDRIEEIKSKFEEMYGLPNCCGAI 183

Query: 219 TCTR----FKIIRNSHFYED-----SIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSST 278
             T        ++ S  + D     S+  Q V D   R L++V G+ G    S +L  S 
Sbjct: 184 DTTHIIMTLPAVQASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSG 243

Query: 279 LFKDIEQGRLLDAPPVYL-HGVAVNQYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAH 338
            FK  E  ++LD  P  L  G  + +Y+ G   YPLLPWL+ P        +  +FN+ H
Sbjct: 244 FFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERH 303

Query: 339 RLMCIPALKAIVSLR-NWGVLSKPM-HEEFKTAVAYIGACSILHNALLMREDF 376
             +   A  A   L+ +W +LSK M   + +   + I  C +LHN ++   D+
Sbjct: 304 EKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 356

BLAST of Bhi01G001266 vs. NCBI nr
Match: KAA0037135.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 803.5 bits (2074), Expect = 8.7e-229
Identity = 397/424 (93.63%), Postives = 413/424 (97.41%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60
           MDSP+LAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDS+FYANLFTHFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSSFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTNP DHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+KQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+ATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300
           SSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YLFG GEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGRGEYPL 300

Query: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360
           LPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 420
           ACSILHNALLMREDFSAMADEWESL+S DHRSQYVE  LN DSTNEKAS+IQRALA RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALAQRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Bhi01G001266 vs. NCBI nr
Match: KGN57516.1 (hypothetical protein Csa_011580 [Cucumis sativus])

HSP 1 Score: 801.2 bits (2068), Expect = 4.3e-228
Identity = 395/424 (93.16%), Postives = 413/424 (97.41%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60
           MDSP+LAALLSSLISQLLLLLFLLFPSSNPHSLFSNS PDS+FYANLF HFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTN SDHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+KQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+ATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300
           SSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YLFGHGEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300

Query: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360
           LPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 420
           ACSILHNALLMREDFSAMADEWESL+S DH+SQYVE  LN DSTNEKAS+IQRALA+RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTNEKASVIQRALALRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Bhi01G001266 vs. NCBI nr
Match: XP_038880641.1 (protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida])

HSP 1 Score: 786.9 bits (2031), Expect = 8.4e-224
Identity = 396/424 (93.40%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60
           MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS
Sbjct: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCT                       
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCT----------------------- 240

Query: 241 SSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300
                SIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL
Sbjct: 241 -----SIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300

Query: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360
           LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG
Sbjct: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 420
           ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 396

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 396

BLAST of Bhi01G001266 vs. NCBI nr
Match: XP_008456140.1 (PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo])

HSP 1 Score: 706.8 bits (1823), Expect = 1.1e-199
Identity = 344/372 (92.47%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 53  FSQDFAASLPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFE 112
           F + FAASLPFLSVSRKRKRTNP DHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFE
Sbjct: 10  FPRIFAASLPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 69

Query: 113 WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAK 172
           WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+K
Sbjct: 70  WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSK 129

Query: 173 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSI 232
           QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+
Sbjct: 130 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 189

Query: 233 ATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYL 292
           ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YL
Sbjct: 190 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 249

Query: 293 FGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEF 352
           FG GEYPLLPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEF
Sbjct: 250 FGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 309

Query: 353 KTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQ 412
           KTAVAYIGACSILHNALLMREDFSAMADEWESL+S DHRSQYVE  LN DSTNEKAS+IQ
Sbjct: 310 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 369

Query: 413 RALAVRARELHS 425
           RALA RARELHS
Sbjct: 370 RALAQRARELHS 381

BLAST of Bhi01G001266 vs. NCBI nr
Match: XP_023536005.1 (protein ALP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671.8 bits (1732), Expect = 3.9e-189
Identity = 348/435 (80.00%), Postives = 370/435 (85.06%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYAN---LFTHFLFSQDF 60
           MDS QLAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DSNFYAN   LF HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNPSDHLELGSS--------HGRFDHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GR  HL RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRV-HLLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFS IS QFGVSESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYE 240
           CAKQLCRVLCTNFRFWVEFPCP+ELELTSSAFED+AGLPNCCGV++CT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSAFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVN 300
                           SIVAGFRGDKDDSTVLMS+TLFKDIE+GRLL +PPVYLHG+AVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEGRLLGSPPVYLHGMAVN 300

Query: 301 QYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMH 360
           QYLFGHGEYPLLPWLM+PFAGAVSGSTEESFN+AHRLMCIPALKAI+SLRNWGVLS+PMH
Sbjct: 301 QYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESLAS DH SQYV   LNEDS +EKAS
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAS 406

Query: 421 IIQRALAVRARELHS 425
           +IQ+ALA+RARELH+
Sbjct: 421 MIQKALALRARELHT 406

BLAST of Bhi01G001266 vs. ExPASy TrEMBL
Match: A0A5D3CRB2 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001410 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.2e-229
Identity = 397/424 (93.63%), Postives = 413/424 (97.41%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60
           MDSP+LAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDS+FYANLFTHFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSSFYANLFTHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTNP DHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+KQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+ATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300
           SSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YLFG GEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGRGEYPL 300

Query: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360
           LPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 420
           ACSILHNALLMREDFSAMADEWESL+S DHRSQYVE  LN DSTNEKAS+IQRALA RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQRALAQRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Bhi01G001266 vs. ExPASy TrEMBL
Match: A0A0A0LBX6 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.1e-228
Identity = 395/424 (93.16%), Postives = 413/424 (97.41%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYANLFTHFLFSQDFAAS 60
           MDSP+LAALLSSLISQLLLLLFLLFPSSNPHSLFSNS PDS+FYANLF HFLFSQDFAAS
Sbjct: 1   MDSPRLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSAPDSSFYANLFAHFLFSQDFAAS 60

Query: 61  LPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120
           LPFLSVSRKRKRTN SDHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP
Sbjct: 61  LPFLSVSRKRKRTNRSDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFEWLSGLLEP 120

Query: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAKQLCRVLCT 180
           LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+KQLCRVLCT
Sbjct: 121 LLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSKQLCRVLCT 180

Query: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSIATQLVVDS 240
           NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+ATQLVVDS
Sbjct: 181 NFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSVATQLVVDS 240

Query: 241 SSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYLFGHGEYPL 300
           SSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YLFGHGEYPL
Sbjct: 241 SSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYLFGHGEYPL 300

Query: 301 LPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEFKTAVAYIG 360
           LPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEFKTAVAYIG
Sbjct: 301 LPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEFKTAVAYIG 360

Query: 361 ACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQRALAVRAR 420
           ACSILHNALLMREDFSAMADEWESL+S DH+SQYVE  LN DSTNEKAS+IQRALA+RAR
Sbjct: 361 ACSILHNALLMREDFSAMADEWESLSSLDHKSQYVEAGLNVDSTNEKASVIQRALALRAR 420

Query: 421 ELHS 425
           ELHS
Sbjct: 421 ELHS 424

BLAST of Bhi01G001266 vs. ExPASy TrEMBL
Match: A0A1S3C2M8 (uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=3 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 5.3e-200
Identity = 344/372 (92.47%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 53  FSQDFAASLPFLSVSRKRKRTNPSDHLELGSSHGRFDHLFRTRTPDSFRNHFRMTSSTFE 112
           F + FAASLPFLSVSRKRKRTNP DHLELGSSHGR  HLFRTRTPDSFRNHFRMTSSTFE
Sbjct: 10  FPRIFAASLPFLSVSRKRKRTNPPDHLELGSSHGRVHHLFRTRTPDSFRNHFRMTSSTFE 69

Query: 113 WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARFCAK 172
           WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFS IS QFGVSESVARFC+K
Sbjct: 70  WLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSTISDQFGVSESVARFCSK 129

Query: 173 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYEDSI 232
           QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVV+CTRFKIIRNSHFYEDS+
Sbjct: 130 QLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVSCTRFKIIRNSHFYEDSV 189

Query: 233 ATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVNQYL 292
           ATQLVVDSSSRILSIVAGFRG+KDDSTVLMSSTLFKDIEQGRLL++PPVYLHGVAVN+YL
Sbjct: 190 ATQLVVDSSSRILSIVAGFRGNKDDSTVLMSSTLFKDIEQGRLLNSPPVYLHGVAVNKYL 249

Query: 293 FGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMHEEF 352
           FG GEYPLLPWL++PFAGAVSGSTEESFN+AHRLMCIPALKAIVSLRNWGVLS+P+HEEF
Sbjct: 250 FGRGEYPLLPWLIVPFAGAVSGSTEESFNEAHRLMCIPALKAIVSLRNWGVLSQPIHEEF 309

Query: 353 KTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKASIIQ 412
           KTAVAYIGACSILHNALLMREDFSAMADEWESL+S DHRSQYVE  LN DSTNEKAS+IQ
Sbjct: 310 KTAVAYIGACSILHNALLMREDFSAMADEWESLSSLDHRSQYVEAGLNVDSTNEKASVIQ 369

Query: 413 RALAVRARELHS 425
           RALA RARELHS
Sbjct: 370 RALAQRARELHS 381

BLAST of Bhi01G001266 vs. ExPASy TrEMBL
Match: A0A6J1FNZ2 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.4e-187
Identity = 344/435 (79.08%), Postives = 369/435 (84.83%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYAN---LFTHFLFSQDF 60
           MDS QLAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DSNFYAN   LF HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNPSDHLELGSS--------HGRFDHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GR  HL RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRV-HLLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFS IS QFGVSESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYE 240
           CAKQLCRVLCTNFRFWVEFPCP+ELELTSS+FED+AGLPNCCGV++CT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSSFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVN 300
                           SIVAGFRGDKDDSTVLMS+TLFKDIE+ RLL +PPVYLHG+AVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEERLLGSPPVYLHGMAVN 300

Query: 301 QYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMH 360
           QYLFGHGEYPLLPWLM+PFAGAVSGSTEESFN+AHRLMCIPALKAI+SLRNWGVLS+PMH
Sbjct: 301 QYLFGHGEYPLLPWLMVPFAGAVSGSTEESFNEAHRLMCIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESLAS DH SQYV   LNEDS +EKA+
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAT 406

Query: 421 IIQRALAVRARELHS 425
           ++Q+ALA+RARELH+
Sbjct: 421 MLQKALALRARELHT 406

BLAST of Bhi01G001266 vs. ExPASy TrEMBL
Match: A0A6J1J0M5 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111480245 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.3e-187
Identity = 346/435 (79.54%), Postives = 368/435 (84.60%), Query Frame = 0

Query: 1   MDSPQLAALLSSLISQLLLLLFLLFPSSNPHSLFSNSTPDSNFYAN---LFTHFLFSQDF 60
           MDS QLAALLSSLISQLLLLL LLFPSSNPHSL SNS+ DSNFYAN   LF HFLFSQ  
Sbjct: 1   MDSRQLAALLSSLISQLLLLLLLLFPSSNPHSLLSNSSSDSNFYANLFPLFNHFLFSQQI 60

Query: 61  AASLPFLSVSRKRKRTNPSDHLELGSS--------HGRFDHLFRTRTPDSFRNHFRMTSS 120
           AASL FLSVSRKRKRT+ S+ LELG S         GR  HL RTR+PDSFRNHFRMTSS
Sbjct: 61  AASLSFLSVSRKRKRTHSSELLELGPSDSGGEDGGRGRV-HLLRTRSPDSFRNHFRMTSS 120

Query: 121 TFEWLSGLLEPLLECRDPVGSPLDLSVEIRLGVGLYRLATGCDFSKISHQFGVSESVARF 180
           TFEWLSGLLEPLLECRDPVGSPLDLS EIRLGVGL RLATGCDFS IS QFGVSESVARF
Sbjct: 121 TFEWLSGLLEPLLECRDPVGSPLDLSAEIRLGVGLSRLATGCDFSTISDQFGVSESVARF 180

Query: 181 CAKQLCRVLCTNFRFWVEFPCPNELELTSSAFEDLAGLPNCCGVVTCTRFKIIRNSHFYE 240
           CAKQLCRVLCTNFRFWVEFPCP+ELELTSSAFED+AGLPNCCGV++CT            
Sbjct: 181 CAKQLCRVLCTNFRFWVEFPCPSELELTSSAFEDIAGLPNCCGVISCT------------ 240

Query: 241 DSIATQLVVDSSSRILSIVAGFRGDKDDSTVLMSSTLFKDIEQGRLLDAPPVYLHGVAVN 300
                           SIVAGFRGDKDDSTVLMS+TLFKDIE+ RLL +PPVYLHGVAVN
Sbjct: 241 ----------------SIVAGFRGDKDDSTVLMSTTLFKDIEEERLLGSPPVYLHGVAVN 300

Query: 301 QYLFGHGEYPLLPWLMLPFAGAVSGSTEESFNKAHRLMCIPALKAIVSLRNWGVLSKPMH 360
           QYLFGHG+YPLLPWLM+PFAGAVSGSTEESFN+AHRLM IPALKAI+SLRNWGVLS+PMH
Sbjct: 301 QYLFGHGDYPLLPWLMVPFAGAVSGSTEESFNEAHRLMSIPALKAIISLRNWGVLSQPMH 360

Query: 361 EEFKTAVAYIGACSILHNALLMREDFSAMADEWESLASFDHRSQYVEDKLNEDSTNEKAS 420
           EEFKTAVAYIGACSILHNALLMREDF+AMADEWESLAS DH SQYV   LNEDS +EKAS
Sbjct: 361 EEFKTAVAYIGACSILHNALLMREDFTAMADEWESLASLDHSSQYVGIGLNEDSPDEKAS 406

Query: 421 IIQRALAVRARELHS 425
           +IQ+ALA+RARELH+
Sbjct: 421 MIQKALALRARELHT 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G55350.12.0e-2928.71PIF / Ping-Pong family of plant transposases [more]
AT3G63270.11.3e-2829.35CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G12010.18.3e-2025.93unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G29780.11.1e-1926.11unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G19120.15.0e-1723.47PIF / Ping-Pong family of plant transposases [more]
Match NameE-valueIdentityDescription
Q9M2U32.8e-2828.71Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K491.8e-2729.35Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
KAA0037135.18.7e-22993.63putative nuclease HARBI1 [Cucumis melo var. makuwa] >TYK13940.1 putative nucleas... [more]
KGN57516.14.3e-22893.16hypothetical protein Csa_011580 [Cucumis sativus][more]
XP_038880641.18.4e-22493.40protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1-like [Benincasa hispida][more]
XP_008456140.11.1e-19992.47PREDICTED: uncharacterized protein LOC103496169 [Cucumis melo][more]
XP_023536005.13.9e-18980.00protein ALP1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5D3CRB24.2e-22993.63Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0LBX62.1e-22893.16DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G202740 PE... [more]
A0A1S3C2M85.3e-20092.47uncharacterized protein LOC103496169 OS=Cucumis melo OX=3656 GN=LOC103496169 PE=... [more]
A0A6J1FNZ21.4e-18779.08protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446927 PE=3 SV=1[more]
A0A6J1J0M52.3e-18779.54protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111480245 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 228..367
e-value: 6.0E-12
score: 45.5
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..423
NoneNo IPR availablePANTHERPTHR22930:SF190OS06G0164500 PROTEINcoord: 1..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001266Bhi01M001266mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding