Bhi01G001264 (gene) Wax gourd (B227) v1

Overview
NameBhi01G001264
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr1: 35187807 .. 35190142 (+)
RNA-Seq ExpressionBhi01G001264
SyntenyBhi01G001264
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCGTGTTCAAATCGGCTGCACTATGATCATTAAGTGGTACCTACATCATCTTTTTTACTAATTTTTCATCATTTACCCTTTTCATTGTGTTTAGCTATGGAAGTAAATAACAATGAATTAGTATGGAGTAAACACGATCAAAGGAATCTAATTCCCTTTACATTGAGACCTATTACAATTGGTACGTGAATGTGAACTCATAATGATTACGACAATTTTAATTACGGAGCAGTAAACGTGGTCGAAATACATTAAATAGAGACAAGCACATAGTGATGACTTGATTGAAGAATACTTGGGTACAGTTGAATACACCCGCCCGTGTACCGGCATAAGTAACTAAAGCTAGTTCCAAGTGTAACTAGGGATAAACCATGGTTAGGTTAAAGAAATAGTTGCACCACACGTGTGGGGGAGAAGTCAGATAAGGGGCAGAGGAACAGAGTTACAGAAGGAGAAGGCAGTGTTTGTTTTCTCTTTTTATCTCTAAAACTCTCTGTTTTGAGTGTTGGGTATGTCTCTCTGTTCCATTCAATTTTAAGAGAGAAGAAGAAACAGAGTCAAAAAAAGGTAAGACTGAAAAATTTGCAGAGTGGTCAGATTTGTTAGTATTAGTATTAGTAGTTCGTCCCCCACTTGTTTGACAAAATTTCTAGCAGAGAGTAAATAGTTATAGAGAAAGAGAATCAAAGCATAAGAAAACATGCCTGGGACTGGGAGTGTTGCTGTTGAACCTTTGGATTTGAGCGTACACATTCCATATCATTTCAGATGCCCAATTTCTCTTGAGCTCATGCGTGATCCTGTCACCGTCAGCACCGGTCAGACCTACGACCGTTCAAGCATAGAGTCATGGGTCGCCACCGGCAACACCACTTGTCCGGTCACTAGGGCTCCACTCACCGACTTCACGCTCATCCCTAACCATACACTGCGCCGGTTAATCCAGGANNNNNNNNNNNNNNNNNNNNACCATACACTGCGCCGGTTAATCCAGGACTGGTGCGTTGCGAATCGGTCTTACGGCGTTGAGCGGATTCCGACTCCGAAGCAACCGGCGGAACCGTCTTTGGTTCGGTCTTTGCTTAGCCAAGCTTCGTCGAGATCGAGTGGTTTGAATTTGAGAATCTCGGCTTTGCGTCGGTTGAAAGGACTCGCCCGTGACTCGGATAAGAATCGGTCTCTGATTTCTTCGCTTAATGCTCGTGAAATTCTTCTGGATGTTGTGTTTTCGAATTTGGATTCGGATTCGGATTCGTTTTCACCTGATTTGAGCCGCGAATCGCTTGCGCTTCTTGTGATGTTACCGCTGACGGAATCTGAGTGTGTTCTTGTTGCGTCTGATCCGCAACGGATTGGTTATCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCGGCGTTGATTGAAACTGTGATTGCCGGAACTCGAGCGTCGGAGCTTCGAAGCCAAATTTGTGGAATCGATGAATTGTTTGAAGGCGTAGTCGAAATTCTCAGAGATCCAACGGCGTATCCACGAGCACTCAAAGTCGGAGTTAAAGCCTTATTCGCTCTCTGTTTGGTGAAACAAACCAGGCACAAGGCAGTTTCCGCCGGCGCCGCCGAGATTATCATCGACCGATTTCCCGATTTGGAGAAGTACGACGCTGAGCGAGCCCTAGCTACGATTGAACTCATTTGCAGAATTCCAACCGGTTGCGATGCGTTTGCAGCTCACGCGCTCACCGTTCCGCTTTTGGTGAAAGTTATTCTGAAGATCTCGGACAGAGCGACGGAGTCAGCTGTCGGAGCTCTGGTGTCGTTATGTTCGGCATCGGAGGAGAATCGGCGGGAGGCGGTGGCGGCGGGGATTTTAACACAGTTGTTGTTGCTGGTGCAAAGTGATTGTACGGAGCGAGTAAAACGGAAATCGCAGGTGCTGCTGAAGCTTCTTCGAGATTCATGGCCGCAAGAATCCGTTGGAAATTCCGACGATTATGGTTGCAGTGAAATTGTGGTACCGTTTTGTTAATTGTGGAGTTTAACATTTTGGATTCTCAAATCTCCATAGATTGTTGGGTAAGTTTGAAGATTCATCAAATGGAACAAAGAACAAAAACTACAGAGAAAGAGAGACAACAAATGGAAGAGATGAAGTTGCAATAGTTTTTCTGTATGTAATTTTTTGTACGTAAATTTTTTTTCCGCTTAATTTCTTTCAACAAAAAAAAAAAAAAAAAAAAAATCCATCTATCGTTAAGTTTTTATTTGTAAGATTTTTGACATTTGTTACATCATTTCCTCTTAATGATATTTTGACATGATTAATTTCCTCAAA

mRNA sequence

CCCGTGTTCAAATCGGCTGCACTATGATCATTAAGTGGTACCTACATCATCTTTTTTACTAATTTTTCATCATTTACCCTTTTCATTGTGTTTAGCTATGGAAGTAAATAACAATGAATTAGTATGGAGTAAACACGATCAAAGGAATCTAATTCCCTTTACATTGAGACCTATTACAATTGGTACGTGAATGTGAACTCATAATGATTACGACAATTTTAATTACGGAGCAGTAAACGTGGTCGAAATACATTAAATAGAGACAAGCACATAGTGATGACTTGATTGAAGAATACTTGGGTACAGTTGAATACACCCGCCCGTGTACCGGCATAAGTAACTAAAGCTAGTTCCAAGTGTAACTAGGGATAAACCATGGTTAGGTTAAAGAAATAGTTGCACCACACGTGTGGGGGAGAAGTCAGATAAGGGGCAGAGGAACAGAGTTACAGAAGGAGAAGGCAGTGTTTGTTTTCTCTTTTTATCTCTAAAACTCTCTGTTTTGAGTGTTGGGTATGTCTCTCTGTTCCATTCAATTTTAAGAGAGAAGAAGAAACAGAGTCAAAAAAAGGTAAGACTGAAAAATTTGCAGAGTGGTCAGATTTGTTAGTATTAGTATTAGTAGTTCGTCCCCCACTTGTTTGACAAAATTTCTAGCAGAGAGTAAATAGTTATAGAGAAAGAGAATCAAAGCATAAGAAAACATGCCTGGGACTGGGAGTGTTGCTGTTGAACCTTTGGATTTGAGCGTACACATTCCATATCATTTCAGATGCCCAATTTCTCTTGAGCTCATGCGTGATCCTGTCACCGTCAGCACCGGTCAGACCTACGACCGTTCAAGCATAGAGTCATGGGTCGCCACCGGCAACACCACTTGTCCGGTCACTAGGGCTCCACTCACCGACTTCACGCTCATCCCTAACCATACACTGCGCCGGTTAATCCAGGANNNNNNNNNNNNNNNNNNNNACCATACACTGCGCCGGTTAATCCAGGACTGGTGCGTTGCGAATCGGTCTTACGGCGTTGAGCGGATTCCGACTCCGAAGCAACCGGCGGAACCGTCTTTGGTTCGGTCTTTGCTTAGCCAAGCTTCGTCGAGATCGAGTGGTTTGAATTTGAGAATCTCGGCTTTGCGTCGGTTGAAAGGACTCGCCCGTGACTCGGATAAGAATCGGTCTCTGATTTCTTCGCTTAATGCTCGTGAAATTCTTCTGGATGTTGTGTTTTCGAATTTGGATTCGGATTCGGATTCGTTTTCACCTGATTTGAGCCGCGAATCGCTTGCGCTTCTTGTGATGTTACCGCTGACGGAATCTGAGTGTGTTCTTGTTGCGTCTGATCCGCAACGGATTGGTTATCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCGGCGTTGATTGAAACTGTGATTGCCGGAACTCGAGCGTCGGAGCTTCGAAGCCAAATTTGTGGAATCGATGAATTGTTTGAAGGCGTAGTCGAAATTCTCAGAGATCCAACGGCGTATCCACGAGCACTCAAAGTCGGAGTTAAAGCCTTATTCGCTCTCTGTTTGGTGAAACAAACCAGGCACAAGGCAGTTTCCGCCGGCGCCGCCGAGATTATCATCGACCGATTTCCCGATTTGGAGAAGTACGACGCTGAGCGAGCCCTAGCTACGATTGAACTCATTTGCAGAATTCCAACCGGTTGCGATGCGTTTGCAGCTCACGCGCTCACCGTTCCGCTTTTGGTGAAAGTTATTCTGAAGATCTCGGACAGAGCGACGGAGTCAGCTGTCGGAGCTCTGGTGTCGTTATGTTCGGCATCGGAGGAGAATCGGCGGGAGGCGGTGGCGGCGGGGATTTTAACACAGTTGTTGTTGCTGGTGCAAAGTGATTGTACGGAGCGAGTAAAACGGAAATCGCAGGTGCTGCTGAAGCTTCTTCGAGATTCATGGCCGCAAGAATCCGTTGGAAATTCCGACGATTATGGTTGCAGTGAAATTGTGGTACCGTTTTGTTAATTGTGGAGTTTAACATTTTGGATTCTCAAATCTCCATAGATTGTTGGGTAAGTTTGAAGATTCATCAAATGGAACAAAGAACAAAAACTACAGAGAAAGAGAGACAACAAATGGAAGAGATGAAGTTGCAATAGTTTTTCTGTATGTAATTTTTTGTACGTAAATTTTTTTTCCGCTTAATTTCTTTCAACAAAAAAAAAAAAAAAAAAAAAATCCATCTATCGTTAAGTTTTTATTTGTAAGATTTTTGACATTTGTTACATCATTTCCTCTTAATGATATTTTGACATGATTAATTTCCTCAAA

Coding sequence (CDS)

ATGCCTGGGACTGGGAGTGTTGCTGTTGAACCTTTGGATTTGAGCGTACACATTCCATATCATTTCAGATGCCCAATTTCTCTTGAGCTCATGCGTGATCCTGTCACCGTCAGCACCGGTCAGACCTACGACCGTTCAAGCATAGAGTCATGGGTCGCCACCGGCAACACCACTTGTCCGGTCACTAGGGCTCCACTCACCGACTTCACGCTCATCCCTAACCATACACTGCGCCGGTTAATCCAGGANNNNNNNNNNNNNNNNNNNNACCATACACTGCGCCGGTTAATCCAGGACTGGTGCGTTGCGAATCGGTCTTACGGCGTTGAGCGGATTCCGACTCCGAAGCAACCGGCGGAACCGTCTTTGGTTCGGTCTTTGCTTAGCCAAGCTTCGTCGAGATCGAGTGGTTTGAATTTGAGAATCTCGGCTTTGCGTCGGTTGAAAGGACTCGCCCGTGACTCGGATAAGAATCGGTCTCTGATTTCTTCGCTTAATGCTCGTGAAATTCTTCTGGATGTTGTGTTTTCGAATTTGGATTCGGATTCGGATTCGTTTTCACCTGATTTGAGCCGCGAATCGCTTGCGCTTCTTGTGATGTTACCGCTGACGGAATCTGAGTGTGTTCTTGTTGCGTCTGATCCGCAACGGATTGGTTATCTGTCACATCTTCTGTTTGATTCTTCGATTGAAGTCCGAATCAATGCCGCGGCGTTGATTGAAACTGTGATTGCCGGAACTCGAGCGTCGGAGCTTCGAAGCCAAATTTGTGGAATCGATGAATTGTTTGAAGGCGTAGTCGAAATTCTCAGAGATCCAACGGCGTATCCACGAGCACTCAAAGTCGGAGTTAAAGCCTTATTCGCTCTCTGTTTGGTGAAACAAACCAGGCACAAGGCAGTTTCCGCCGGCGCCGCCGAGATTATCATCGACCGATTTCCCGATTTGGAGAAGTACGACGCTGAGCGAGCCCTAGCTACGATTGAACTCATTTGCAGAATTCCAACCGGTTGCGATGCGTTTGCAGCTCACGCGCTCACCGTTCCGCTTTTGGTGAAAGTTATTCTGAAGATCTCGGACAGAGCGACGGAGTCAGCTGTCGGAGCTCTGGTGTCGTTATGTTCGGCATCGGAGGAGAATCGGCGGGAGGCGGTGGCGGCGGGGATTTTAACACAGTTGTTGTTGCTGGTGCAAAGTGATTGTACGGAGCGAGTAAAACGGAAATCGCAGGTGCTGCTGAAGCTTCTTCGAGATTCATGGCCGCAAGAATCCGTTGGAAATTCCGACGATTATGGTTGCAGTGAAATTGTGGTACCGTTTTGTTAA

Protein sequence

MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQESVGNSDDYGCSEIVVPFC
Homology
BLAST of Bhi01G001264 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 527.3 bits (1357), Expect = 1.2e-149
Identity = 277/444 (62.39%), Postives = 346/444 (77.93%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPG     +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCP
Sbjct: 1   MPGN----LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTR  L+DFTLIPN                HTLRRLIQ+WCVANRS GVERIPTPKQPA+
Sbjct: 61  VTRVALSDFTLIPN----------------HTLRRLIQEWCVANRSNGVERIPTPKQPAD 120

Query: 121 PSLVRSLLSQASSRSS---GLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFS 180
           P  VRSLLSQAS+ +     +  R +A+RRL+GLARDS+KNR LI+  NAREIL+ ++F+
Sbjct: 121 PISVRSLLSQASAITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA 180

Query: 181 NLDSDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAA 240
             D ++ S S +L  ESLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAA
Sbjct: 181 --DIETTSLSSELVSESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAA 240

Query: 241 ALIETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTR 300
           ALIE V+ G ++ +L+  I G D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTR
Sbjct: 241 ALIEMVLTGAKSMDLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTR 300

Query: 301 HKAVSAGAAEIIIDRF-PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVIL 360
           H A+SAGA  I+IDR   D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL
Sbjct: 301 HLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTIL 360

Query: 361 KISDRATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLL 420
           ++SDRATE A GAL++LC+A E  R EA AAG++TQLLLLVQSDCTER KRK+Q+LLKLL
Sbjct: 361 RVSDRATEYAAGALLALCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 420

Query: 421 RDSWPQESVGNSDDYGCSEIVVPF 441
           RDSWP +S  +SDD+  SE V PF
Sbjct: 421 RDSWPDDSTVHSDDFNRSE-VAPF 421

BLAST of Bhi01G001264 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 511.9 bits (1317), Expect = 5.0e-145
Identity = 273/439 (62.19%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 9   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 68
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 69  DFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL 128
           DFTLIPN                HTLRRLIQ+WCVANRS GVERIPTPKQPA+P+ VR+L
Sbjct: 65  DFTLIPN----------------HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRAL 124

Query: 129 LSQASSRSS---GLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSD 188
           LSQAS+ +     +  R +ALRRL+G ARDSDKNR LI++ NA EIL+ ++F      S+
Sbjct: 125 LSQASAITGTHVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------SE 184

Query: 189 SFSPDLSRESLALLVMLPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETV 248
           + S +L  ESLALLVMLP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIE V
Sbjct: 185 TTSSELVSESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIV 244

Query: 249 IAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSA 308
             GT++++L+  I   + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++A
Sbjct: 245 STGTKSADLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITA 304

Query: 309 GAAEIIIDRF-PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRA 368
           GA EI+IDR   D ++ D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRA
Sbjct: 305 GAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRA 364

Query: 369 TESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ 428
           TE A GAL++LC+A E  R EA  AG++ QLLL+VQS+CTER K+K+Q LLKLLRDSWP 
Sbjct: 365 TEYAAGALLALCTAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 421

Query: 429 -ESVGNSDDYGCSEIVVPF 441
             S  NSDD+GCS  VVPF
Sbjct: 425 YNSFANSDDFGCSSQVVPF 421

BLAST of Bhi01G001264 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-48
Identity = 135/419 (32.22%), Postives = 213/419 (50.84%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 72  LIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQ 131
           L PN                HTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  
Sbjct: 66  LTPN----------------HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRD 125

Query: 132 ASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPDL 191
           ++S       ++  L+RL+ +  ++  N+  + +    E L ++V       +DS +  L
Sbjct: 126 SASSHEN---QVKCLKRLRQIVSENATNKRCLEAAGVPEFLANIV------SNDSENGSL 185

Query: 192 SRESLALLVMLPLTES---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGT 251
           + E+L LL  L  +E+     +    D   +  L+ ++     E R+ A  L++ ++   
Sbjct: 186 TDEALNLLYHLETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL--- 245

Query: 252 RASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAE 311
             ++    +    E+F  VV+IL D  +  +A K  +  L  +C   + RHKAV AG   
Sbjct: 246 EVADPMQSMTLKPEVFTEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVIS 305

Query: 312 IIIDRFPD----LEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRAT 371
           +II+   D     E+   E A+  ++L+C+   G   F  H   + ++ K IL++S  A+
Sbjct: 306 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 365

Query: 372 ESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 421
           + AV  L+S+    A+     E +  G++ +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Bhi01G001264 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 186.4 bits (472), Expect = 4.9e-47
Identity = 141/442 (31.90%), Postives = 224/442 (50.68%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 72  LIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQ 131
           L PN                HTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ +
Sbjct: 61  LTPN----------------HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKE 120

Query: 132 ASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD---SDSDSFS 191
           +S  SS LN ++  L+RL+ +  ++  N+  + +    E L ++V +++D   S S S S
Sbjct: 121 SS--SSHLN-QVKCLKRLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLS 180

Query: 192 P----------------DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHL 251
                            D SR  +  AL V+  L  SE  L +          +  L+ +
Sbjct: 181 SSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKI 240

Query: 252 LFDSSIEVRINAAALIETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGV 311
           +     E R  AA L++ ++     ++    I    ELF  V++IL D  ++ +A +  +
Sbjct: 241 MQRGIYESRAYAALLLKKLL---EVADPMQIILLERELFGEVIQILHDQISH-KATRSAM 300

Query: 312 KALFALCLVKQTRHKAVSAGAAEIIIDRFPD----LEKYDAERALATIELICRIPTGCDA 371
           + L   C   + RHKAV  G   +II+   D     E+ ++E A+  ++++C+   G   
Sbjct: 301 QILVITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAE 360

Query: 372 FAAHALTVPLLVKVILKISDRATESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQ 421
           F  H   + ++ K IL++S   +E AV  L+S+    A+    +E +  G++ +L L++Q
Sbjct: 361 FLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ 419

BLAST of Bhi01G001264 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.1e-42
Identity = 128/412 (31.07%), Postives = 207/412 (50.24%), Query Frame = 0

Query: 16  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 75
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPN 
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN- 88

Query: 76  TLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL---LSQAS 135
                          HT+R++IQ WCV   S  ++RIPTP+ P  P  V  +   LS A+
Sbjct: 89  ---------------HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSAT 148

Query: 136 SRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPD--- 195
            R       +  + ++K L  +S+KNR  ++  +   +L D          D FS D   
Sbjct: 149 RRGDYEKCGV-IIEKIKKLGDESEKNRKCVNENSVGWVLCDCF--------DKFSGDEKL 208

Query: 196 --LSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGT 255
             +  E L+LL  +     E +   +       ++ LL  +   VR NAA +++ +++  
Sbjct: 209 TFMLNEILSLLTWMFPIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS-- 268

Query: 256 RASELRSQICGIDE-LFEGVVEILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGA 315
              E R     ++  + E +V+++RD  +   + K  + A++ + L K +   + +  G 
Sbjct: 269 -LDETRVHSFAVENGVAEALVKLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGL 328

Query: 316 AEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATES 375
             I ++   D E    E+ALA ++ IC    G +    +AL +PLLVK I K+S+ AT S
Sbjct: 329 VSITVEMIVDAENSVCEKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRS 388

Query: 376 AVGALVSLCSASEENR-REAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLL 417
           ++  ++ L          +AV  G   ++LL++Q    E  K K+  LLK++
Sbjct: 389 SMSMILKLWKTGNTVAVEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMM 411

BLAST of Bhi01G001264 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.6e-148
Identity = 277/444 (62.39%), Postives = 346/444 (77.93%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPG     +EPLDL + IPYHFRCPISL+LM DPVT+STGQTYDR+SI+SW+A GNTTCP
Sbjct: 1   MPGN----LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTR  L+DFTLIPN                HTLRRLIQ+WCVANRS GVERIPTPKQPA+
Sbjct: 61  VTRVALSDFTLIPN----------------HTLRRLIQEWCVANRSNGVERIPTPKQPAD 120

Query: 121 PSLVRSLLSQASSRSS---GLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFS 180
           P  VRSLLSQAS+ +     +  R +A+RRL+GLARDS+KNR LI+  NAREIL+ ++F+
Sbjct: 121 PISVRSLLSQASAITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFA 180

Query: 181 NLDSDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAA 240
             D ++ S S +L  ESLALLV+L +TE+EC  VASDP R+G+++ LLFDSSIE+R+NAA
Sbjct: 181 --DIETTSLSSELVSESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAA 240

Query: 241 ALIETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTR 300
           ALIE V+ G ++ +L+  I G D +FEGV+++L++P +  RALK+G+KA+FALCLVKQTR
Sbjct: 241 ALIEMVLTGAKSMDLKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTR 300

Query: 301 HKAVSAGAAEIIIDRF-PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVIL 360
           H A+SAGA  I+IDR   D ++ D ER LAT+EL+CR+P GC AF  HALTVPL+VK IL
Sbjct: 301 HLAISAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTIL 360

Query: 361 KISDRATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLL 420
           ++SDRATE A GAL++LC+A E  R EA AAG++TQLLLLVQSDCTER KRK+Q+LLKLL
Sbjct: 361 RVSDRATEYAAGALLALCTAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLL 420

Query: 421 RDSWPQESVGNSDDYGCSEIVVPF 441
           RDSWP +S  +SDD+  SE V PF
Sbjct: 421 RDSWPDDSTVHSDDFNRSE-VAPF 421

BLAST of Bhi01G001264 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 7.1e-144
Identity = 273/439 (62.19%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 9   VEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLT 68
           +EPLDL + IPYHFRCPISLELM+DPVTV TGQTYDR+SIESWV+ G NTTCPVTRAPL+
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 69  DFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL 128
           DFTLIPN                HTLRRLIQ+WCVANRS GVERIPTPKQPA+P+ VR+L
Sbjct: 65  DFTLIPN----------------HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRAL 124

Query: 129 LSQASSRSS---GLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSD 188
           LSQAS+ +     +  R +ALRRL+G ARDSDKNR LI++ NA EIL+ ++F      S+
Sbjct: 125 LSQASAITGTHVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILF------SE 184

Query: 189 SFSPDLSRESLALLVMLPLTE-SECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETV 248
           + S +L  ESLALLVMLP+TE ++ V ++SDP R+ +L+ LLFDSSIE R+NAAALIE V
Sbjct: 185 TTSSELVSESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIV 244

Query: 249 IAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSA 308
             GT++++L+  I   + +FEGV+++LR+P +  RALK+G+K LFALC VK TRH A++A
Sbjct: 245 STGTKSADLKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITA 304

Query: 309 GAAEIIIDRF-PDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRA 368
           GA EI+IDR   D ++ D ERALAT+EL+CR P GC AF  HALTVPLLVK IL++SDRA
Sbjct: 305 GAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRA 364

Query: 369 TESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ 428
           TE A GAL++LC+A E  R EA  AG++ QLLL+VQS+CTER K+K+Q LLKLLRDSWP 
Sbjct: 365 TEYAAGALLALCTAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 421

Query: 429 -ESVGNSDDYGCSEIVVPF 441
             S  NSDD+GCS  VVPF
Sbjct: 425 YNSFANSDDFGCSSQVVPF 421

BLAST of Bhi01G001264 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 135/419 (32.22%), Postives = 213/419 (50.84%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISLE+M+DPV VSTG TYDR SIE W+  G   +CPVT+  +TD  
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 72  LIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQ 131
           L PN                HTLRRLIQ WC  N SYGVERIPTP+ P   S +  L+  
Sbjct: 66  LTPN----------------HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRD 125

Query: 132 ASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPDL 191
           ++S       ++  L+RL+ +  ++  N+  + +    E L ++V       +DS +  L
Sbjct: 126 SASSHEN---QVKCLKRLRQIVSENATNKRCLEAAGVPEFLANIV------SNDSENGSL 185

Query: 192 SRESLALLVMLPLTES---ECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGT 251
           + E+L LL  L  +E+     +    D   +  L+ ++     E R+ A  L++ ++   
Sbjct: 186 TDEALNLLYHLETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNIL--- 245

Query: 252 RASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAGAAE 311
             ++    +    E+F  VV+IL D  +  +A K  +  L  +C   + RHKAV AG   
Sbjct: 246 EVADPMQSMTLKPEVFTEVVQILDDRIS-QKATKAAMHILVNICPWGRNRHKAVEAGVIS 305

Query: 312 IIIDRFPD----LEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRAT 371
           +II+   D     E+   E A+  ++L+C+   G   F  H   + ++ K IL++S  A+
Sbjct: 306 VIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTAS 365

Query: 372 ESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 421
           + AV  L+S+    A+     E +  G++ +L L++Q  C  + K K++ LLKL    W
Sbjct: 366 DRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVW 395

BLAST of Bhi01G001264 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.8e-46
Identity = 141/442 (31.90%), Postives = 224/442 (50.68%), Query Frame = 0

Query: 12  LDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATG-NTTCPVTRAPLTDFT 71
           +D  + IP  F CPISL++M+DPV VSTG TYDR SIE W+ +G   +CPVT+  +T+  
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 72  LIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSLLSQ 131
           L PN                HTLRRLIQ WC  N SYG+ERIPTPK P   S +  L+ +
Sbjct: 61  LTPN----------------HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKE 120

Query: 132 ASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD---SDSDSFS 191
           +S  SS LN ++  L+RL+ +  ++  N+  + +    E L ++V +++D   S S S S
Sbjct: 121 SS--SSHLN-QVKCLKRLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLS 180

Query: 192 P----------------DLSRESL--ALLVMLPLTESECVLVA-----SDPQRIGYLSHL 251
                            D SR  +  AL V+  L  SE  L +          +  L+ +
Sbjct: 181 SSNLNDMCQSNMLENRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKI 240

Query: 252 LFDSSIEVRINAAALIETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGV 311
           +     E R  AA L++ ++     ++    I    ELF  V++IL D  ++ +A +  +
Sbjct: 241 MQRGIYESRAYAALLLKKLL---EVADPMQIILLERELFGEVIQILHDQISH-KATRSAM 300

Query: 312 KALFALCLVKQTRHKAVSAGAAEIIIDRFPD----LEKYDAERALATIELICRIPTGCDA 371
           + L   C   + RHKAV  G   +II+   D     E+ ++E A+  ++++C+   G   
Sbjct: 301 QILVITCPWGRNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAE 360

Query: 372 FAAHALTVPLLVKVILKISDRATESAVGALVSL--CSASEENRREAVAAGILTQLLLLVQ 421
           F  H   + ++ K IL++S   +E AV  L+S+    A+    +E +  G++ +L L++Q
Sbjct: 361 FLNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ 419

BLAST of Bhi01G001264 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 8.7e-41
Identity = 128/412 (31.07%), Postives = 207/412 (50.24%), Query Frame = 0

Query: 16  VHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRAPLTDFTLIPNH 75
           + IP  F+CPIS++LM+DPV +STG TYDR SIE+W+ +GN TCPVT   LT F  IPN 
Sbjct: 29  ITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIPN- 88

Query: 76  TLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLVRSL---LSQAS 135
                          HT+R++IQ WCV   S  ++RIPTP+ P  P  V  +   LS A+
Sbjct: 89  ---------------HTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSAT 148

Query: 136 SRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSDSFSPD--- 195
            R       +  + ++K L  +S+KNR  ++  +   +L D          D FS D   
Sbjct: 149 RRGDYEKCGV-IIEKIKKLGDESEKNRKCVNENSVGWVLCDCF--------DKFSGDEKL 208

Query: 196 --LSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVIAGT 255
             +  E L+LL  +     E +   +       ++ LL  +   VR NAA +++ +++  
Sbjct: 209 TFMLNEILSLLTWMFPIGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILS-- 268

Query: 256 RASELRSQICGIDE-LFEGVVEILRDPTAYPRALKVGVKALFALCLVK-QTRHKAVSAGA 315
              E R     ++  + E +V+++RD  +   + K  + A++ + L K +   + +  G 
Sbjct: 269 -LDETRVHSFAVENGVAEALVKLIRDSVS-SSSTKSSLIAIYQMVLQKPEIASEFLEIGL 328

Query: 316 AEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATES 375
             I ++   D E    E+ALA ++ IC    G +    +AL +PLLVK I K+S+ AT S
Sbjct: 329 VSITVEMIVDAENSVCEKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRS 388

Query: 376 AVGALVSLCSASEENR-REAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLL 417
           ++  ++ L          +AV  G   ++LL++Q    E  K K+  LLK++
Sbjct: 389 SMSMILKLWKTGNTVAVEDAVRLGAFQKVLLVLQVGYGEETKEKATELLKMM 411

BLAST of Bhi01G001264 vs. NCBI nr
Match: XP_038875248.1 (U-box domain-containing protein 26-like isoform X1 [Benincasa hispida])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDYGCSEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 441

BLAST of Bhi01G001264 vs. NCBI nr
Match: XP_038875257.1 (U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_038875267.1 U-box domain-containing protein 26-like isoform X3 [Benincasa hispida])

HSP 1 Score: 807.4 bits (2084), Expect = 6.2e-230
Identity = 425/441 (96.37%), Postives = 425/441 (96.37%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDYGCSEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 425

BLAST of Bhi01G001264 vs. NCBI nr
Match: XP_008456137.1 (PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa] >TYK13948.1 U-box domain-containing protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 795.0 bits (2052), Expect = 3.2e-226
Identity = 417/441 (94.56%), Postives = 422/441 (95.69%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLGQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           S SDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SGSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELR+QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRTQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDYGCSEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 425

BLAST of Bhi01G001264 vs. NCBI nr
Match: XP_004140703.1 (U-box domain-containing protein 26 [Cucumis sativus] >KGN57520.1 hypothetical protein Csa_011013 [Cucumis sativus])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 415/441 (94.10%), Postives = 421/441 (95.46%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQ+WCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQEWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL+QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLAQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           S SDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALI
Sbjct: 181 SGSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELR+QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRTQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDY CSEIVVPFC
Sbjct: 421 PQESVGNSDDYACSEIVVPFC 425

BLAST of Bhi01G001264 vs. NCBI nr
Match: XP_022981075.1 (U-box domain-containing protein 26-like [Cucurbita maxima])

HSP 1 Score: 745.3 bits (1923), Expect = 2.9e-211
Identity = 394/436 (90.37%), Postives = 407/436 (93.35%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLV 124
           PLT+FTLIPN                HTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLV
Sbjct: 63  PLTEFTLIPN----------------HTLRRLIQEWCVANRSYGVERIPTPKQPADPSLV 122

Query: 125 RSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSD 184
           RSLL QASS+SSG + RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSD
Sbjct: 123 RSLLGQASSKSSGSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSD 182

Query: 185 SFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVI 244
           S SPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VI
Sbjct: 183 SVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVI 242

Query: 245 AGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAG 304
           AGTRASELR QIC IDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAG
Sbjct: 243 AGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAG 302

Query: 305 AAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATE 364
           AAEI+IDRFPDLEKYDAERALATIELICRIPTG DAFAAHALTVPLLVKVILKISDRATE
Sbjct: 303 AAEILIDRFPDLEKYDAERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATE 362

Query: 365 SAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQES 424
           SAVGALVSLCSASEENRREAVAAGI TQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQ+S
Sbjct: 363 SAVGALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDS 420

Query: 425 VGNSDDYGCSEIVVPF 441
           VGNSDDYGCSEIV+PF
Sbjct: 423 VGNSDDYGCSEIVIPF 420

BLAST of Bhi01G001264 vs. ExPASy TrEMBL
Match: A0A5A7T4S1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001500 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 417/441 (94.56%), Postives = 422/441 (95.69%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLGQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           S SDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SGSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELR+QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRTQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDYGCSEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 425

BLAST of Bhi01G001264 vs. ExPASy TrEMBL
Match: A0A1S3C380 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 417/441 (94.56%), Postives = 422/441 (95.69%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQDWCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQDWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLGQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           S SDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI
Sbjct: 181 SGSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELR+QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRTQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICR+PTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRVPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDYGCSEIVVPFC
Sbjct: 421 PQESVGNSDDYGCSEIVVPFC 425

BLAST of Bhi01G001264 vs. ExPASy TrEMBL
Match: A0A0A0L6F8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.5e-225
Identity = 415/441 (94.10%), Postives = 421/441 (95.46%), Query Frame = 0

Query: 1   MPGTGSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60
           MPGTGSVAV+PLDLS+HIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP
Sbjct: 1   MPGTGSVAVQPLDLSLHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCP 60

Query: 61  VTRAPLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAE 120
           VTRAPLTDFTLIPN                HTLRRLIQ+WCVANRSYGVERIPTPKQPAE
Sbjct: 61  VTRAPLTDFTLIPN----------------HTLRRLIQEWCVANRSYGVERIPTPKQPAE 120

Query: 121 PSLVRSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180
           PSLVRSLL+QASSRSSG +LRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD
Sbjct: 121 PSLVRSLLAQASSRSSGSSLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLD 180

Query: 181 SDSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALI 240
           S SDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIE RINAAALI
Sbjct: 181 SGSDSFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEDRINAAALI 240

Query: 241 ETVIAGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300
           ETVIAGTRASELR+QICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA
Sbjct: 241 ETVIAGTRASELRTQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKA 300

Query: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360
           VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD
Sbjct: 301 VSAGAAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISD 360

Query: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420
           RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW
Sbjct: 361 RATESAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSW 420

Query: 421 PQESVGNSDDYGCSEIVVPFC 442
           PQESVGNSDDY CSEIVVPFC
Sbjct: 421 PQESVGNSDDYACSEIVVPFC 425

BLAST of Bhi01G001264 vs. ExPASy TrEMBL
Match: A0A6J1IYF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 PE=4 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 394/436 (90.37%), Postives = 407/436 (93.35%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLV 124
           PLT+FTLIPN                HTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLV
Sbjct: 63  PLTEFTLIPN----------------HTLRRLIQEWCVANRSYGVERIPTPKQPADPSLV 122

Query: 125 RSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSD 184
           RSLL QASS+SSG + RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSD
Sbjct: 123 RSLLGQASSKSSGSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSD 182

Query: 185 SFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVI 244
           S SPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VI
Sbjct: 183 SVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVI 242

Query: 245 AGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAG 304
           AGTRASELR QIC IDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAG
Sbjct: 243 AGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAG 302

Query: 305 AAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATE 364
           AAEI+IDRFPDLEKYDAERALATIELICRIPTG DAFAAHALTVPLLVKVILKISDRATE
Sbjct: 303 AAEILIDRFPDLEKYDAERALATIELICRIPTGYDAFAAHALTVPLLVKVILKISDRATE 362

Query: 365 SAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQES 424
           SAVGALVSLCSASEENRREAVAAGI TQLLLLVQSDCT+RVKRKSQVLLKLLRDSWPQ+S
Sbjct: 363 SAVGALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTDRVKRKSQVLLKLLRDSWPQDS 420

Query: 425 VGNSDDYGCSEIVVPF 441
           VGNSDDYGCSEIV+PF
Sbjct: 423 VGNSDDYGCSEIVIPF 420

BLAST of Bhi01G001264 vs. ExPASy TrEMBL
Match: A0A6J1FSF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739 PE=4 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 394/436 (90.37%), Postives = 407/436 (93.35%), Query Frame = 0

Query: 5   GSVAVEPLDLSVHIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 64
           GSV VEPLDLSV IPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA
Sbjct: 3   GSVVVEPLDLSVQIPYHFRCPISLELMRDPVTVSTGQTYDRSSIESWVATGNTTCPVTRA 62

Query: 65  PLTDFTLIPNHTLRRLIQXXXXXXXXHTLRRLIQDWCVANRSYGVERIPTPKQPAEPSLV 124
           PLT+FTLIPN                HTLRRLIQ+WCVANRSYGVERIPTPKQPA+PSLV
Sbjct: 63  PLTEFTLIPN----------------HTLRRLIQEWCVANRSYGVERIPTPKQPADPSLV 122

Query: 125 RSLLSQASSRSSGLNLRISALRRLKGLARDSDKNRSLISSLNAREILLDVVFSNLDSDSD 184
           RSLL QASS+SSG + RISA+RRLKGLARDS+KNRSLISSLNAREIL+DVVFSN  SDSD
Sbjct: 123 RSLLGQASSKSSGSSSRISAVRRLKGLARDSEKNRSLISSLNAREILIDVVFSN--SDSD 182

Query: 185 SFSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIETVI 244
           S SPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIE VI
Sbjct: 183 SVSPDLSRESLALLVMLPLTESECVLVASDPQRIGYLSHLLFDSSIEVRINAAALIEIVI 242

Query: 245 AGTRASELRSQICGIDELFEGVVEILRDPTAYPRALKVGVKALFALCLVKQTRHKAVSAG 304
           AGTRASELR QIC IDELFEGVVEILRDPTAYPRA+KVGVKALFALCLVKQTRHKAVSAG
Sbjct: 243 AGTRASELRGQICAIDELFEGVVEILRDPTAYPRAVKVGVKALFALCLVKQTRHKAVSAG 302

Query: 305 AAEIIIDRFPDLEKYDAERALATIELICRIPTGCDAFAAHALTVPLLVKVILKISDRATE 364
           AAEI+IDRFPDLEKYDAERALATIELICRIPTG DAFA+HALTVPLLVKVILKISDRATE
Sbjct: 303 AAEILIDRFPDLEKYDAERALATIELICRIPTGYDAFASHALTVPLLVKVILKISDRATE 362

Query: 365 SAVGALVSLCSASEENRREAVAAGILTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQES 424
           SAVGALVSLCSASEENRREAVAAGI TQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQ+S
Sbjct: 363 SAVGALVSLCSASEENRREAVAAGIFTQLLLLVQSDCTERVKRKSQVLLKLLRDSWPQDS 420

Query: 425 VGNSDDYGCSEIVVPF 441
           VGNSDDYGCSEIV+PF
Sbjct: 423 VGNSDDYGCSEIVIPF 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G49780.11.2e-14962.39plant U-box 26 [more]
AT3G19380.15.0e-14562.19plant U-box 25 [more]
AT2G35930.11.2e-4832.22plant U-box 23 [more]
AT3G52450.14.9e-4731.90plant U-box 22 [more]
AT5G37490.16.1e-4231.07ARM repeat superfamily protein [more]
Match NameE-valueIdentityDescription
Q9FXA41.6e-14862.39U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9LT797.1e-14462.19U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q84TG31.6e-4732.22E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC66.8e-4631.90E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q5PNY68.7e-4131.07U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
XP_038875248.11.2e-236100.00U-box domain-containing protein 26-like isoform X1 [Benincasa hispida][more]
XP_038875257.16.2e-23096.37U-box domain-containing protein 26-like isoform X2 [Benincasa hispida] >XP_03887... [more]
XP_008456137.13.2e-22694.56PREDICTED: U-box domain-containing protein 26-like [Cucumis melo] >KAA0037126.1 ... [more]
XP_004140703.11.8e-22494.10U-box domain-containing protein 26 [Cucumis sativus] >KGN57520.1 hypothetical pr... [more]
XP_022981075.12.9e-21190.37U-box domain-containing protein 26-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A5A7T4S11.6e-22694.56RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C3801.6e-22694.56RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103496167 PE=4 ... [more]
A0A0A0L6F88.5e-22594.10RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G203270 PE... [more]
A0A6J1IYF71.4e-21190.37RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480329 P... [more]
A0A6J1FSF91.4e-21190.37RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447739... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 21..84
e-value: 3.6E-33
score: 126.2
IPR003613U box domainPFAMPF04564U-boxcoord: 18..83
e-value: 8.2E-20
score: 70.8
IPR003613U box domainPROSITEPS51698U_BOXcoord: 17..91
score: 39.188217
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 100..306
e-value: 2.3E-8
score: 35.6
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 326..433
e-value: 3.0E-6
score: 28.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 12..99
e-value: 1.5E-27
score: 97.5
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 82..429
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 9..82
NoneNo IPR availablePANTHERPTHR22849:SF141RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 82..429
NoneNo IPR availablePANTHERPTHR22849:SF141RING-TYPE E3 UBIQUITIN TRANSFERASEcoord: 9..82
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 22..63
e-value: 4.25539E-25
score: 94.993
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 15..100
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 115..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M001264Bhi01M001264mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity